GSE66441-GSM1622468-GPL17439-PMID:26258987.tsv 2.08 KB
"strain: K-12 MG1655"	"characteristics_ch1.1"
"genotype: gadX-8myc"	"characteristics_ch1.2"
"chip antibody: anti-myc (Santa Cruz Biotech, sc-28207)"	"characteristics_ch1.3"
"ChIP-exo reads were aligned to the ASM584v2 genome reference sequence using using bowtie v1.0.0 with parameters  -S"	"data_processing.1"
"MACE software (https://code.google.com/p/chip-exo/) was used to detect peaks with aligned output from bowtie mapping."	"data_processing.2"
"Read count was calculated for each genomic position from sequence alignment, and 95% strongest intensity was used as background intensity."	"data_processing.3"
"For each peak detected with MACE, binding intensity was calculated by averaging read counts from two biological replicates and dividing by background intensity."	"data_processing.4"
"Genome_build: ASM584v2"	"data_processing.5"
"Supplementary_files_format_and_content: Tab-delimited text files in gff format which has 8 columns: sequence id, source(empty), feature (+/- strand), start position, end position, intensity score, strand(+/-), frame(.), attribute(.)."	"data_processing.6"
"ChIP-seq libraries were prepared for sequencing using standard Illumina protocols"	"extract_protocol_ch1.1"
"E. coli K-12 MG1655 WT, GadE-8-myc, GadW-8-myc, and GadX-8-myc tagged strains were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5."	"growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"Immunoprecipitated DNA"	"source_name_ch1.1"
"ChIP-exo GadX pH5.5 2"	"title.1"
"ChIP-Seq"	"library_strategy.1"
"strain: K-12 MG1655"	"characteristics_ch1.1"
"genotype: gadX-8myc"	"characteristics_ch1.2"
"chip antibody: anti-myc (Santa Cruz Biotech, sc-28207)"	"characteristics_ch1.3"
"E. coli K-12 MG1655 WT, GadE-8-myc, GadW-8-myc, and GadX-8-myc tagged strains were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5."	"growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"Immunoprecipitated DNA"	"source_name_ch1.1"