GSE6609-GSM153320-GPL4698-PMID:17891379.tsv 3.11 KB
"pool of aliquots from all samples"	"characteristics_ch1.1"
"day: . strain: . oxygen: ."	"characteristics_ch2.1"
"Feature pixel median minus background pixel mean;"	"data_processing.1"
"ratio set to missing where SNR (feature mean - backround mean /  background SD) less than 2 in both channels ;"	"data_processing.2"
"normalization subtracting array-median log2 ratio."	"data_processing.3"
"Cells were harvested on days 1 and 3 of glucose starvation, with samples taken from the"	"extract_protocol_ch1.1"
"same cultures used for the viability assay. For each experimental condition, 15 aliquots of"	"extract_protocol_ch1.2"
"1.8 ml (ca. 3E+9 cells) were pipetted into 2.0 ml tubes and immediately centrifuged at"	"extract_protocol_ch1.3"
"16,000 g for 40 s at room temperature.  The supernatant was discarded, and cell pellets"	"extract_protocol_ch1.4"
"were immediately frozen in liquid nitrogen and stored at -80 deg. C.  For RNA isolation,"	"extract_protocol_ch1.5"
"triplicate samples were prepared by combining cells from 5 tubes each.  Each pellet was"	"extract_protocol_ch1.6"
"re-suspended in 200 ul Trizol and combined for a total volume of 1 ml, then promptly"	"extract_protocol_ch1.7"
"chilled in a dry ice/ethanol. bath.  After homogenization with glass beads,. RNA was"	"extract_protocol_ch1.8"
"isolated by chloroform extraction and precipitation by isopropanol. Precipitated RNA"	"extract_protocol_ch1.9"
"was washed with 70 percent ethanol, resuspended in 30 ul water, and held at 4 deg. C for"	"extract_protocol_ch1.10"
"further processing"	"extract_protocol_ch1.11"
"Cells were harvested on days 1 and 3 of glucose starvation, with samples taken from the"	"extract_protocol_ch2.1"
"same cultures used for the viability assay. For each experimental condition, 15 aliquots of"	"extract_protocol_ch2.2"
"1.8 ml (ca. 3E+9 cells) were pipetted into 2.0 ml tubes and immediately centrifuged at"	"extract_protocol_ch2.3"
"16,000 g for 40 s at room temperature.  The supernatant was discarded, and cell pellets"	"extract_protocol_ch2.4"
"were immediately frozen in liquid nitrogen and stored at -80 deg. C.  For RNA isolation,"	"extract_protocol_ch2.5"
"triplicate samples were prepared by combining cells from 5 tubes each.  Each pellet was"	"extract_protocol_ch2.6"
"re-suspended in 200 ul Trizol and combined for a total volume of 1 ml, then promptly"	"extract_protocol_ch2.7"
"chilled in a dry ice/ethanol. bath.  After homogenization with glass beads,. RNA was"	"extract_protocol_ch2.8"
"isolated by chloroform extraction and precipitation by isopropanol. Precipitated RNA"	"extract_protocol_ch2.9"
"was washed with 70 percent ethanol, resuspended in 30 ul water, and held at 4 deg. C for"	"extract_protocol_ch2.10"
"further processing"	"extract_protocol_ch2.11"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"Reference Pool"	"source_name_ch1.1"
"D3+AN13"	"source_name_ch2.1"
"68933"	"title.1"
"pool of aliquots from all samples"	"characteristics_ch1.1"
"day: . strain: . oxygen: ."	"characteristics_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"Reference Pool"	"source_name_ch1.1"
"D3+AN13"	"source_name_ch2.1"