GSE65642-GSM1602353-GPL16085-PMID:29394395.tsv
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"strain: K-12 MG1655" "characteristics_ch1.1"
"genotype/variation: delta_cra" "characteristics_ch1.2"
"carbon source: glucose" "characteristics_ch1.3"
"Sequenced reads were mapped onto NC_000913 reference genome sequence using bowtie v1.0.0 with parameters -X 1000 -n 2 -3 3 -S" "data_processing.1"
"Fragments Per Kilobase of exon per Megabase of library size (FPKM) were calculated using cufflinks v.1.3.0" "data_processing.2"
"Genome_build: NC_000913" "data_processing.3"
"Supplementary_files_format_and_content: comma-delimited text files include FPKM values for each Sample." "data_processing.4"
"Total RNA was extracted using the RNeasy Plus Mini kit (Qiagen Inc., Valencia, CA, USA) and genomic DNA was removed by gDNA Eliminator spin column in the RNeasy Plus Mini Kit. RNA quality and concentration was determined by analysis with a NanoDrop 1000 (Thermo Scientific Inc., Wilmington, DE, USA)." "extract_protocol_ch1.1"
"RNA libraries were prepared for sequencing using standard Illumina protocols" "extract_protocol_ch1.2"
"E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"Δcra_glucose" "source_name_ch1.1"
"Δcra glucose 1" "title.1"
"The cell culture was treated with the RNAprotect reagent (Qiagen)." "treatment_protocol_ch1.1"
"RNA-Seq" "library_strategy.1"
"strain: K-12 MG1655" "characteristics_ch1.1"
"genotype/variation: delta_cra" "characteristics_ch1.2"
"carbon source: glucose" "characteristics_ch1.3"
"E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"Δcra_glucose" "source_name_ch1.1"
"The cell culture was treated with the RNAprotect reagent (Qiagen)." "treatment_protocol_ch1.1"