"MG1655 rpoHp3::lacZ delta lacX74 hfq2::omega(Km;KpnI) (hfq+)/ptrc99A (control vector)"	"characteristics_ch1.1"
"MG1655 rpoHp3::lacZ delta lacX74 hfq2::omega(Km;KpnI) (hfq+)/pLC245 (vector carrying inducible Ptrc::rpoE)"	"characteristics_ch2.1"
"Data filtered for PCR success, >3x local background and spot quality (GenePix Flag). Normalized using Lowess smoothing from MA plot"	"data_processing.1"
"Culture samples for microarray analysis were added to ice-cold 5% water-saturated phenol in ethanol solution, centrifuged at 6,600 g and the cell pellets flash frozen in liquid N2 before storing at -80 degrees C until required. Total RNA was isolated from the stored cell pellets using the hot phenol method, and labeled Cy3 and Cy5 cDNA was prepared from 16 ug total RNA with 10 ug random hexamer (Integrated DNA Technologies, Inc., Coralville, IA, USA)."	"extract_protocol_ch1.1"
"Culture samples for microarray analysis were added to ice-cold 5% water-saturated phenol in ethanol solution, centrifuged at 6,600 g and the cell pellets flash frozen in liquid N2 before storing at -80 degrees C until required. Total RNA was isolated from the stored cell pellets using the hot phenol method, and labeled Cy3 and Cy5 cDNA was prepared from 16 ug total RNA with 10 ug random hexamer (Integrated DNA Technologies, Inc., Coralville, IA, USA)."	"extract_protocol_ch2.1"
"M9 minimal complete media, cultures grown aerobically at 30 degrees C in a gyratory water bath shaking at 240 rpm"	"growth_protocol_ch1.1"
"M9 minimal complete media, cultures grown aerobically at 30 degrees C in a gyratory water bath shaking at 240 rpm"	"growth_protocol_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"Wild type control (20 min)"	"source_name_ch1.1"
"RpoE induced (20 min)"	"source_name_ch2.1"
"EC18n018 hfq+ rpoE overexpression 20 min"	"title.1"
"At OD450 = 0.3, cultures induced with 1 mM IPTG. Cells harvested 20 min after induction"	"treatment_protocol_ch1.1"
"At OD450 = 0.3, cultures induced with 1 mM IPTG. Cells harvested 20 min after induction"	"treatment_protocol_ch2.1"
"MG1655 rpoHp3::lacZ delta lacX74 hfq2::omega(Km;KpnI) (hfq+)/ptrc99A (control vector)"	"characteristics_ch1.1"
"MG1655 rpoHp3::lacZ delta lacX74 hfq2::omega(Km;KpnI) (hfq+)/pLC245 (vector carrying inducible Ptrc::rpoE)"	"characteristics_ch2.1"
"M9 minimal complete media, cultures grown aerobically at 30 degrees C in a gyratory water bath shaking at 240 rpm"	"growth_protocol_ch1.1"
"M9 minimal complete media, cultures grown aerobically at 30 degrees C in a gyratory water bath shaking at 240 rpm"	"growth_protocol_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"Wild type control (20 min)"	"source_name_ch1.1"
"RpoE induced (20 min)"	"source_name_ch2.1"
"At OD450 = 0.3, cultures induced with 1 mM IPTG. Cells harvested 20 min after induction"	"treatment_protocol_ch1.1"
"At OD450 = 0.3, cultures induced with 1 mM IPTG. Cells harvested 20 min after induction"	"treatment_protocol_ch2.1"