GSE6444-GSM147989-GPL2928-GPL3500-GPL4620-PMID:17158661.tsv
2.45 KB
"MG1655 rpoHp3::lacZ delta lacX74" "characteristics_ch1.1"
"MG1655 rpoHp3::lacZ delta lacX74 nadB::Tn10 drseA" "characteristics_ch2.1"
"Data filtered for PCR success, >3x local background and spot quality (GenePix Flag). Normalized using Lowess smoothing from MA plot" "data_processing.1"
"Culture samples for microarray analysis were added to ice-cold 5% water-saturated phenol in ethanol solution, centrifuged at 6,600 g and the cell pellets flash frozen in liquid N2 before storing at -80 degrees C until required. Total RNA was isolated from the stored cell pellets using the hot phenol method, and labeled Cy3 and Cy5 cDNA was prepared from 16 ug total RNA with 10 ug random hexamer (Integrated DNA Technologies, Inc., Coralville, IA, USA)." "extract_protocol_ch1.1"
"Culture samples for microarray analysis were added to ice-cold 5% water-saturated phenol in ethanol solution, centrifuged at 6,600 g and the cell pellets flash frozen in liquid N2 before storing at -80 degrees C until required. Total RNA was isolated from the stored cell pellets using the hot phenol method, and labeled Cy3 and Cy5 cDNA was prepared from 16 ug total RNA with 10 ug random hexamer (Integrated DNA Technologies, Inc., Coralville, IA, USA)." "extract_protocol_ch2.1"
"M9 minimal complete media, cultures grown aerobically at 30 degrees C in a gyratory water bath shaking at 240 rpm" "growth_protocol_ch1.1"
"M9 minimal complete media, cultures grown aerobically at 30 degrees C in a gyratory water bath shaking at 240 rpm" "growth_protocol_ch2.1"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli" "organism_ch2.1"
"Wild type control" "source_name_ch1.1"
"Delta rseA" "source_name_ch2.1"
"EC19n101 delta rseA" "title.1"
"Cells harvested at OD450 = 0.3" "treatment_protocol_ch1.1"
"Cells harvested at OD450 = 0.3" "treatment_protocol_ch2.1"
"MG1655 rpoHp3::lacZ delta lacX74" "characteristics_ch1.1"
"MG1655 rpoHp3::lacZ delta lacX74 nadB::Tn10 drseA" "characteristics_ch2.1"
"M9 minimal complete media, cultures grown aerobically at 30 degrees C in a gyratory water bath shaking at 240 rpm" "growth_protocol_ch1.1"
"M9 minimal complete media, cultures grown aerobically at 30 degrees C in a gyratory water bath shaking at 240 rpm" "growth_protocol_ch2.1"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli" "organism_ch2.1"
"Wild type control" "source_name_ch1.1"
"Delta rseA" "source_name_ch2.1"
"Cells harvested at OD450 = 0.3" "treatment_protocol_ch1.1"
"Cells harvested at OD450 = 0.3" "treatment_protocol_ch2.1"