GSE61736-GSM1512001-GPL19219-PMID:.tsv 1.52 KB
"strain: MG1655"	"characteristics_ch1.1"
"treatment: Oxidative stress"	"characteristics_ch1.2"
"The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleGen Deva software, version 1.2 (Roche NimbleGen, Inc.)."	"data_processing.1"
"Total RNA was extracted using the RNeasy Mini kit (Qiagen) and DNA was removed by on-column DNase digestion with the RNase-Free DNase set (Qiagen). RNA  concentration was measured by SpectorPhotometer(NonoDrop)."	"extract_protocol_ch1.1"
"cDNA were synthesized from the reverse transcription of Total RNA using the Life Technologies SuperScript II Double-Strand Synthesis Kit"	"extract_protocol_ch1.2"
"E. coli MG1655 Oxidative Stress was grown untill reach to half of O.D.600nm of Standard sample at 37°C in 49ml MOPS media with 400μl 7% Hydrogen peroxide."	"growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"E. coli MG1655 Oxidative stress"	"source_name_ch1.1"
"MG1655_under_Oxidative_Stress_rep1"	"title.1"
"strain: MG1655"	"characteristics_ch1.1"
"treatment: Oxidative stress"	"characteristics_ch1.2"
"E. coli MG1655 Oxidative Stress was grown untill reach to half of O.D.600nm of Standard sample at 37°C in 49ml MOPS media with 400μl 7% Hydrogen peroxide."	"growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"E. coli MG1655 Oxidative stress"	"source_name_ch1.1"