GSE61604-GSM1509313-GPL3154-PMID:25577644.tsv 1.78 KB
"strain: BW25113"	"characteristics_ch1.1"
"genotype/variation: wild type"	"characteristics_ch1.2"
"incubated with: GST alone (3 μM; control)"	"characteristics_ch1.3"
"incubation duration: 10 min"	"characteristics_ch1.4"
"CEL files were quantified with Distribution Free Weighted method (DFW), using the statistical language R (R-2.7.2) and Bioconductor 2.2."	"data_processing.1"
"Total RNA was purified with an RNeasy micro kit (Qiagen K.K., Tokyo, Japan)."	"extract_protocol_ch1.1"
"E. coli BW25113 were maintained in Luria-Bertani medium at 37ºC for about 14 h until they reached the stationary phase of cell growth before treatment."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E. coli incubated with GST protein alone for 10 min"	"source_name_ch1.1"
"E. coli incubated with GST protein for 10 min, biological rep2"	"title.1"
"The E. coli strain BW25113 (2 x 10^9) suspended with insect saline (0.13 M NaCl, 4.7 mM KCl, 1.9 mM CaCl2), was incubated with GST or GST-fused Drosophila immune proteins for 10 min at room temperature."	"treatment_protocol_ch1.1"
"strain: BW25113"	"characteristics_ch1.1"
"genotype/variation: wild type"	"characteristics_ch1.2"
"incubated with: GST alone (3 μM; control)"	"characteristics_ch1.3"
"incubation duration: 10 min"	"characteristics_ch1.4"
"E. coli BW25113 were maintained in Luria-Bertani medium at 37ºC for about 14 h until they reached the stationary phase of cell growth before treatment."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E. coli incubated with GST protein alone for 10 min"	"source_name_ch1.1"
"The E. coli strain BW25113 (2 x 10^9) suspended with insect saline (0.13 M NaCl, 4.7 mM KCl, 1.9 mM CaCl2), was incubated with GST or GST-fused Drosophila immune proteins for 10 min at room temperature."	"treatment_protocol_ch1.1"