GSE6100-GSM141485-GPL4374-PMID:.tsv
1.89 KB
"Strain: W3110" "characteristics_ch1.1"
"OD :1.7" "characteristics_ch1.2"
"Strain :W3110" "characteristics_ch2.1"
"OD :2.7" "characteristics_ch2.2"
"Normalization is performed by TREBAX, using MA-plots. MA-plots can reveal the spot artifacts globally and show the intensity-dependent logarithmic ratio of raw microarray data. In MA-plots, we calculate two parameters, average of logarithmic transferred intensity As=(log(Ts)+log(Rs))/2 and logarithmic ratio of intensity Ms=log(Ts/Rs) Here, Ts and Rs are the intensity of target and control experiments for sth spot, respectively.By plotting values of As on the abscissa and Ms on the ordinate of a coordinate system, it is possible to evaluate the bias error with respects to the average logarithmic intensities. Normalized log ratio M’’s is estimated as the difference between Ms and baseline M’s. Here, using a relation between Ms and As, (Ms=f(As)+es, es is the difference between Ms and f(As) for gene s) by MA plot; the baseline for sth spot is estimated by M’=f(A). The genes whose signal intensity is regarded as zero are eliminated in the present analysis. With this methodology, it is assumed that there is no large error due to expression intensity in the majority of the spots and that expression change does not occur on the majority of the spots." "data_processing.1"
"Qiagen RNeasy mini Kit" "extract_protocol_ch1.1"
"Qiagen RNeasy mini Kit" "extract_protocol_ch2.1"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli" "organism_ch2.1"
"time 7 (OD=1.7)" "source_name_ch1.1"
"time 8 (OD=2.7)" "source_name_ch2.1"
"Sequential Design timecourse t7_t8.2" "title.1"
"Strain: W3110" "characteristics_ch1.1"
"OD :1.7" "characteristics_ch1.2"
"Strain :W3110" "characteristics_ch2.1"
"OD :2.7" "characteristics_ch2.2"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli" "organism_ch2.1"
"time 7 (OD=1.7)" "source_name_ch1.1"
"time 8 (OD=2.7)" "source_name_ch2.1"