GSE58977-GSM1423257-GPL13634-PMID:.tsv
2.48 KB
"strain: GLBRCE1" "characteristics_ch1.1"
"medium: SynH_Acids_Amides" "characteristics_ch1.2"
"growth phase: Stat" "characteristics_ch1.3"
"Probe signal intensities were preprocessed using robust multichip averaging (RMA) in the program ArrayStar (DNASTAR), and the resulting gene expression signals were quantile-normalized across all samples using the normalize.quantiles function in the Bioconductor package for R." "data_processing.1"
"RNA was obtained from cells pellets by lysozyme treatment and phenol-chloroform extraction, analyzed by agarose gel electrophoresis to confirm integrity, quantified using a Nanodrop spectrophotometer (Thermo Scientific), and stored at -80°C" "extract_protocol_ch1.1"
"Fermentations were carried out in 3L bioreactors (Applikon Biotechnology) containing 2.45L of ACSH, SynH, SynH+LT, or SynH + Acid/Amide LTs, and cultures were diluted into ACSH or SynH with initial OD at 0.2, grown anaerobically overnight, and then inoculated into bioreactors to a starting OD600 of 0.2" "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli GLBRCE1" "source_name_ch1.1"
"X520_V4_T8_475585A01" "title.1"
"Cells (10 ml) for transcriptomic analysis were collected into tubes containing 1.25 ml ice-cold 5% (vol/vol) unbuffered phenol in ethanol (EP) and pelleted by centrifugation (10,000 g, 4°C, 3 min). To remove residual traces of hydrolysate, cell pellets were twice resuspended in ice-cold GMM plus 0.125 volume of EP, repelleted, then flash frozen in dry ice-ethanol, and stored at -80°C." "treatment_protocol_ch1.1"
"strain: GLBRCE1" "characteristics_ch1.1"
"medium: SynH_Acids_Amides" "characteristics_ch1.2"
"growth phase: Stat" "characteristics_ch1.3"
"Fermentations were carried out in 3L bioreactors (Applikon Biotechnology) containing 2.45L of ACSH, SynH, SynH+LT, or SynH + Acid/Amide LTs, and cultures were diluted into ACSH or SynH with initial OD at 0.2, grown anaerobically overnight, and then inoculated into bioreactors to a starting OD600 of 0.2" "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli GLBRCE1" "source_name_ch1.1"
"Cells (10 ml) for transcriptomic analysis were collected into tubes containing 1.25 ml ice-cold 5% (vol/vol) unbuffered phenol in ethanol (EP) and pelleted by centrifugation (10,000 g, 4°C, 3 min). To remove residual traces of hydrolysate, cell pellets were twice resuspended in ice-cold GMM plus 0.125 volume of EP, repelleted, then flash frozen in dry ice-ethanol, and stored at -80°C." "treatment_protocol_ch1.1"