GSE55719-GSM1342416-GPL13336-PMID:25564530.tsv
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"strain: DH1 ΔhisI::cat ΔintC::Ptrc-dsred.t4-tetR-PEM7-zeo ΔgalK::PtetA-gfpuv5" "characteristics_ch1.1"
"condition: histidine supplied, 1 mM" "characteristics_ch1.2"
"Microarray data were processed using custom scripts written in R based on the finite hybridisation (FH) model (Ono et al, 2008) and the thermodynamic model of non-specific binding (NSB) on short nucleotide microarrays (Furusawa et al, 2009). The log10 mRNA concentration (pM) data are provided as a supplementary file on the SERIES record." "data_processing.1"
"Total RNAs were extracted using an RNeasy mini kit (Qiagen) in accordance with the manufacturer’s instructions." "extract_protocol_ch1.1"
"Cells were grown in the minimal media, modified M63. The final cell concentrations were controlled ~ 10^8 cells/mL within the exponential growth phase." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"OSU12-hisI, histidine supplied, 1 mM" "source_name_ch1.1"
"OSU12-hisI, histidine supplied, 1 mM, rep 3" "title.1"
"The cell culture was put into cold phenol-ethanol solution (1 g of phenol in 10 mL of ethanol) prepared in advance. The cells were collected by centrifugation at 16,000 × g for 5 min at 4°C, and the pelleted cells were stored at –80°C prior to use." "treatment_protocol_ch1.1"
"strain: DH1 ΔhisI::cat ΔintC::Ptrc-dsred.t4-tetR-PEM7-zeo ΔgalK::PtetA-gfpuv5" "characteristics_ch1.1"
"condition: histidine supplied, 1 mM" "characteristics_ch1.2"
"Cells were grown in the minimal media, modified M63. The final cell concentrations were controlled ~ 10^8 cells/mL within the exponential growth phase." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"OSU12-hisI, histidine supplied, 1 mM" "source_name_ch1.1"
"The cell culture was put into cold phenol-ethanol solution (1 g of phenol in 10 mL of ethanol) prepared in advance. The cells were collected by centrifugation at 16,000 × g for 5 min at 4°C, and the pelleted cells were stored at –80°C prior to use." "treatment_protocol_ch1.1"