"chip antibody: anti-FLAG antibody (Sigma Cat #: F3165-1MG)"	"characteristics_ch1.1"
"strain: K-12"	"characteristics_ch1.2"
"reads aligned to the reference genome of E. coli K-12 Mg1655 using BWA, with quality threshold of 20"	"data_processing.1"
"Reads mapped to more than one locus were discarded. The mapped position obtained from the SAM output file from BWA lists was taken and the position was extended to 280 bp which is the average length of DNA fragments submitted for sequencing."	"data_processing.2"
"For each co-ordinate coverage was calculated as the number of reads which was aligned againist the position. The coverage was normalized and converted into a z score. Z score threshold of 12 was selected to identify H-NS binding regions."	"data_processing.3"
"The binding regions were considered by taking into account base position with z-score greater than threshold and merging adjacent binding regions seperated by a distance of less than 200bo into the single binding regions"	"data_processing.4"
"consensus binding regions were obtained as the portion which was covered by replicate samples"	"data_processing.5"
"genome build: ASM584v1"	"data_processing.6"
"Supplementary_files_format_and_content: format:bed format"	"data_processing.7"
"Supplementary_files_format_and_content: contents : reference seq,co-ordinate start position,co-ordinate end position,counts for the position, mapping quality, strand position"	"data_processing.8"
"Standard illumina library construction protocol for ChIP"	"extract_protocol_ch1.1"
"MG1655 hns-FLAG cells were grown in liquid LB medium with and without bicyclomycin (BCM) at 37 degree with shaking at 200rpm"	"growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"H-NS flag tagged cells without bcm treatment"	"source_name_ch1.1"
"ChIP_hns-F_bcm-#2"	"title.1"
"Two biological replicates were performed for samples with and without treatment with bicyclomycin. For samples without BCM treatment, cells were grown OD600 ≈ 0.8 at 370 with shaking at 200 rpm.  For samples with BCM treatment, cells were grown to early log phase (O.D. 600=0.1) ≈ 1h at 370 with shaking at 200 rpm. BCM was added to a final concentration of 25ug mL-1"	"treatment_protocol_ch1.1"
"ChIP-Seq"	"library_strategy.1"
"chip antibody: anti-FLAG antibody (Sigma Cat #: F3165-1MG)"	"characteristics_ch1.1"
"strain: K-12"	"characteristics_ch1.2"
"MG1655 hns-FLAG cells were grown in liquid LB medium with and without bicyclomycin (BCM) at 37 degree with shaking at 200rpm"	"growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"H-NS flag tagged cells without bcm treatment"	"source_name_ch1.1"
"Two biological replicates were performed for samples with and without treatment with bicyclomycin. For samples without BCM treatment, cells were grown OD600 ≈ 0.8 at 370 with shaking at 200 rpm.  For samples with BCM treatment, cells were grown to early log phase (O.D. 600=0.1) ≈ 1h at 370 with shaking at 200 rpm. BCM was added to a final concentration of 25ug mL-1"	"treatment_protocol_ch1.1"