"strain: EC472/01 (isolated from cattle)"	"characteristics_ch1.1"
"e.coli culture protocol: conditioned Caco-2 cell medium"	"characteristics_ch1.2"
"The scanned images were analyzed with Feature Extraction Software version 9.5.3.1 (Agilent) using default parameters (protocol GE1-v5_95 and Grid: 020097_D_F_20080627) to obtain background subtracted and spatially detrended Processed Signal intensities but not included the normalization process.  Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded."	"data_processing.1"
"STEC strains were lysate using lysozyme (1 mg/mL) and proteinase K (2 mg/mL) and incubated for 10 min at 20°C. Total RNA was obtained using the RNeasy Mini Kit (Qiagen cat no. 74104, Valencia, CA) according to the manufacturer's instructions. The purity and quantification for all RNA samples were done using the NanoVue spectrophotometer and the RNA quality was assessed on the Agilent BioAnalyzer 2100 (Agilent, Santa Clara, CA). All samples that present RIN > 7.0 were stored at -80°C until used in hybridization experiments."	"extract_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"STEC EC472/01, conditioned medium"	"source_name_ch1.1"
"STEC EC472 after 3 hrs incubation in conditioned medium, replicate 4 [COND]"	"title.1"
"The conditioned medium (C) was the DMEM medium containing 10% FBS without antibiotics recovered after 24 hrs incubation with differentiated Caco-2 cells. 400 µL of STEC culture were inoculated in 4 mL of C medium and incubated for 3 hrs at 37°C. After this period the bacteria were recovered by centrifugation for 10 min at 5000 xg and the pellet was ressuspended in 600 µL of RNAprotect Bacteria Reagent (Qiagen cat. no. 76506, Valencia, CA) for RNA extraction."	"treatment_protocol_ch1.1"
"strain: EC472/01 (isolated from cattle)"	"characteristics_ch1.1"
"e.coli culture protocol: conditioned Caco-2 cell medium"	"characteristics_ch1.2"
"Escherichia coli"	"organism_ch1.1"
"STEC EC472/01, conditioned medium"	"source_name_ch1.1"
"The conditioned medium (C) was the DMEM medium containing 10% FBS without antibiotics recovered after 24 hrs incubation with differentiated Caco-2 cells. 400 µL of STEC culture were inoculated in 4 mL of C medium and incubated for 3 hrs at 37°C. After this period the bacteria were recovered by centrifugation for 10 min at 5000 xg and the pellet was ressuspended in 600 µL of RNAprotect Bacteria Reagent (Qiagen cat. no. 76506, Valencia, CA) for RNA extraction."	"treatment_protocol_ch1.1"