GSE44273-GSM1081694-GPL3154-PMID:.tsv
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"strain: E. coli RP437" "characteristics_ch1.1"
"cell type: Persister cells" "characteristics_ch1.2"
"GeneChip Operating Software (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method, genes with a p-value of less than 0.0025 or greater than 0.9975 were considered statistically significant based on Wilcoxon signed rank test and Tukey Byweight, labeled as I and D in the attached txt file. To ensure the significance of microarray data, an additional criterion was applied to only select the genes with an expression ratio of 2 or high from this group as induced and repressed genes." "data_processing.1"
"RNeasy Mini Kit (Qiagen, Austin, TX, USA) with on-column DNase treatment (RNase-Free DNase Set, Qiagen)." "extract_protocol_ch1.1"
"RP437 was inoculated in 100 mL LB and grown for 18 h (200 rpm, 37oC). Persister cells were isolated by lysing the normal cells with 5 µg/mL Ofloxacin (3.5h, 200 rpm, 37oC), followed by centrifugation and washing with 0.85% NaCl solution." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"RP437 persisters treated with BF8 at 5 µg/mL for 1 hour" "source_name_ch1.1"
"F1" "title.1"
"The isolated persisters were incubated in 0.85% NaCl solution for 1 h (200 rpm, 37oC) with or without 5 µg/mL BF8." "treatment_protocol_ch1.1"
"strain: E. coli RP437" "characteristics_ch1.1"
"cell type: Persister cells" "characteristics_ch1.2"
"RP437 was inoculated in 100 mL LB and grown for 18 h (200 rpm, 37oC). Persister cells were isolated by lysing the normal cells with 5 µg/mL Ofloxacin (3.5h, 200 rpm, 37oC), followed by centrifugation and washing with 0.85% NaCl solution." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"RP437 persisters treated with BF8 at 5 µg/mL for 1 hour" "source_name_ch1.1"
"The isolated persisters were incubated in 0.85% NaCl solution for 1 h (200 rpm, 37oC) with or without 5 µg/mL BF8." "treatment_protocol_ch1.1"