GSE4321-GSM98728-GPL3500-PMID:16818608.tsv
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"MG1655 PhtpG::lacZ delta lacX74/ptrc99A (control vector)" "characteristics_ch1.1"
"MG1655 PhtpG::lacZ delta lacX74/pTG2 (vector carrying inducible Ptrc::rpoH)" "characteristics_ch2.1"
"Data filtered for PCR success, >3x local background and spot quality (GenePix Flag). Normalized using Lowess smoothing from MA plot" "data_processing.1"
"Culture samples for microarray analysis were added to ice-cold 5% water-saturated phenol in ethanol solution, centrifuged at 6,600 g and the cell pellets flash frozen in liquid N2 before storing at -80 degrees C until required. Total RNA was isolated from the stored cell pellets using the hot phenol method, and labeled Cy3 and Cy5 cDNA was prepared from 16 ug total RNA with 10 ug random hexamer (Integrated DNA Technologies, Inc., Coralville, IA, USA)." "extract_protocol_ch1.1"
"Culture samples for microarray analysis were added to ice-cold 5% water-saturated phenol in ethanol solution, centrifuged at 6,600 g and the cell pellets flash frozen in liquid N2 before storing at -80 degrees C until required. Total RNA was isolated from the stored cell pellets using the hot phenol method, and labeled Cy3 and Cy5 cDNA was prepared from 16 ug total RNA with 10 ug random hexamer (Integrated DNA Technologies, Inc., Coralville, IA, USA)." "extract_protocol_ch2.1"
"M9 minimal complete media, cultures grown aerobically at 30 degrees C in a gyratory water bath shaking at 240 rpm" "growth_protocol_ch1.1"
"M9 minimal complete media, cultures grown aerobically at 30 degrees C in a gyratory water bath shaking at 240 rpm" "growth_protocol_ch2.1"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli" "organism_ch2.1"
"Wild type control (2.5 min)" "source_name_ch1.1"
"RpoH induced (2.5 min)" "source_name_ch2.1"
"EC18n183 RpoH 2.5 min Time course 3" "title.1"
"At OD450 = 0.3, cultures induced with 1 mM IPTG. Cells harvested 2.5 min after induction" "treatment_protocol_ch1.1"
"At OD450 = 0.3, cultures induced with 1 mM IPTG. Cells harvested 2.5 min after induction" "treatment_protocol_ch2.1"
"MG1655 PhtpG::lacZ delta lacX74/ptrc99A (control vector)" "characteristics_ch1.1"
"MG1655 PhtpG::lacZ delta lacX74/pTG2 (vector carrying inducible Ptrc::rpoH)" "characteristics_ch2.1"
"M9 minimal complete media, cultures grown aerobically at 30 degrees C in a gyratory water bath shaking at 240 rpm" "growth_protocol_ch1.1"
"M9 minimal complete media, cultures grown aerobically at 30 degrees C in a gyratory water bath shaking at 240 rpm" "growth_protocol_ch2.1"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli" "organism_ch2.1"
"Wild type control (2.5 min)" "source_name_ch1.1"
"RpoH induced (2.5 min)" "source_name_ch2.1"
"At OD450 = 0.3, cultures induced with 1 mM IPTG. Cells harvested 2.5 min after induction" "treatment_protocol_ch1.1"
"At OD450 = 0.3, cultures induced with 1 mM IPTG. Cells harvested 2.5 min after induction" "treatment_protocol_ch2.1"