GSE41938-GSM1027905-GPL8708-PMID:23207917.tsv 1.7 KB
"strain: MDS42 (RL1961)"	"characteristics_ch1.1"
"genotype/variation: 42 deletions"	"characteristics_ch1.2"
"treated with: 20 ug/ml bicyclomycin"	"characteristics_ch1.3"
"Raw probe intensities were normalized across samples using RMA analysis implemented in the NimbleScan software package (v.2.4)."	"data_processing.1"
"Total RNA was extracted from cell pellets by hot phenol extraction. The integrity of total RNA was determined from agarose gel or microchannel electrophoretograms."	"extract_protocol_ch1.1"
"Cells were grown in MOPS minimal medium with 0.2% glucose at 37 oC  in gas-sparged Roux bottles or shaking flasks to mid-log phase (OD600 ~ 0.3-0.4)."	"growth_protocol_ch1.1"
"Escherichia coli K-12"	"organism_ch1.1"
"MDS42 + 20 ug/ml bicyclomycin"	"source_name_ch1.1"
"MDS42 + 20 ug/ml bicyclomycin 1"	"title.1"
"Cultures were transferred directly into an ice-cold ethanol/phenol stop solution, which immediately inactivated cellular RNases. Cells were collected by centrifugation and stored at -80 oC until RNA extraction."	"treatment_protocol_ch1.1"
"strain: MDS42 (RL1961)"	"characteristics_ch1.1"
"genotype/variation: 42 deletions"	"characteristics_ch1.2"
"treated with: 20 ug/ml bicyclomycin"	"characteristics_ch1.3"
"Cells were grown in MOPS minimal medium with 0.2% glucose at 37 oC  in gas-sparged Roux bottles or shaking flasks to mid-log phase (OD600 ~ 0.3-0.4)."	"growth_protocol_ch1.1"
"Escherichia coli K-12"	"organism_ch1.1"
"MDS42 + 20 ug/ml bicyclomycin"	"source_name_ch1.1"
"Cultures were transferred directly into an ice-cold ethanol/phenol stop solution, which immediately inactivated cellular RNases. Cells were collected by centrifugation and stored at -80 oC until RNA extraction."	"treatment_protocol_ch1.1"