GSE41938-GSM1027905-GPL8708-PMID:23207917.tsv
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"strain: MDS42 (RL1961)" "characteristics_ch1.1"
"genotype/variation: 42 deletions" "characteristics_ch1.2"
"treated with: 20 ug/ml bicyclomycin" "characteristics_ch1.3"
"Raw probe intensities were normalized across samples using RMA analysis implemented in the NimbleScan software package (v.2.4)." "data_processing.1"
"Total RNA was extracted from cell pellets by hot phenol extraction. The integrity of total RNA was determined from agarose gel or microchannel electrophoretograms." "extract_protocol_ch1.1"
"Cells were grown in MOPS minimal medium with 0.2% glucose at 37 oC in gas-sparged Roux bottles or shaking flasks to mid-log phase (OD600 ~ 0.3-0.4)." "growth_protocol_ch1.1"
"Escherichia coli K-12" "organism_ch1.1"
"MDS42 + 20 ug/ml bicyclomycin" "source_name_ch1.1"
"MDS42 + 20 ug/ml bicyclomycin 1" "title.1"
"Cultures were transferred directly into an ice-cold ethanol/phenol stop solution, which immediately inactivated cellular RNases. Cells were collected by centrifugation and stored at -80 oC until RNA extraction." "treatment_protocol_ch1.1"
"strain: MDS42 (RL1961)" "characteristics_ch1.1"
"genotype/variation: 42 deletions" "characteristics_ch1.2"
"treated with: 20 ug/ml bicyclomycin" "characteristics_ch1.3"
"Cells were grown in MOPS minimal medium with 0.2% glucose at 37 oC in gas-sparged Roux bottles or shaking flasks to mid-log phase (OD600 ~ 0.3-0.4)." "growth_protocol_ch1.1"
"Escherichia coli K-12" "organism_ch1.1"
"MDS42 + 20 ug/ml bicyclomycin" "source_name_ch1.1"
"Cultures were transferred directly into an ice-cold ethanol/phenol stop solution, which immediately inactivated cellular RNases. Cells were collected by centrifugation and stored at -80 oC until RNA extraction." "treatment_protocol_ch1.1"