"treatment: treated sinusoidal continous magnetic field"	"characteristics_ch1.1"
"treatment duration: 15 h"	"characteristics_ch1.2"
"growth condition: steady-state continuous cultivation (dilution rate of 0.4 h-1), aerobic"	"characteristics_ch1.3"
"medium: defined mineral medium with 1 g/l glucose"	"characteristics_ch1.4"
"Quality control and microarray data analysis of all 60 chips was done using R/Bioconductor (http://www.bioconductor.org/). Signal intensity files (CEL files) of all 60 chips were summarized using RMA (robust multi array average) as processing algorithm and quantile normalization. A modified t-test using limma was performed for comparison of experimental conditions. See also additional files containing only E.coli K-12 MG1655 probes with P-values and ratio."	"data_processing.1"
"Samples of ~7.2 ml bacterial culture were mixed with 0.8 ml stop solution, pelleted and total RNA was purified using RNeasy Mini Kit (Qiagen). DNase treatment was done twice with RQ1 DNase (Promega) to remove genomic DNA. cDNA synthesis was done according to the GeneChip® Expression Analysis Technical Manual of Affymetrix using M-MLV reverse transcriptase (Promega), without DTT addition. 13 µl of EB buffer were used for elution of cDNA after its clean-up with the MiniElute CR purification Kit (Qiagen). cDNA fragmentation was done in a 20-µl reaction containing 3.5 µg cDNA, 2 µl 10x One Phor-All buffer, 2 µl DNase I (diluted to 0.2 U/ µl, Amersham Pharmacia Biotech) in nuclease-free water. After incubation for 4 min at 37°C, DNase I was heat inactivated at 98°C for 10 min."	"extract_protocol_ch1.1"
"Escherichia coli K-12 strain MG1655 was cultivated aerobically (~0.5 l/min air supply) in a defined mineral medium containing 1 g/l glucose in continous cultures growing in a chemostat (dilution rate = 0.4 h-1, working volume = 100 ml)."	"growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"E_coli_treated_sinusoidal_cont_15h_rep1"	"source_name_ch1.1"
"E_coli_treated_sinusoidal_cont_15h_rep1"	"title.1"
"Samples of magnetic field-treated or sham-treated cells were withdrawn as fast as possible from the bioreactor (not later than 3 min) after the specified treatement period. Samples taken during intermittent exposure were withdrawn in the 4 min exposure break. Unexposed samples were withdrawn as fast as possible during steady-state conditions in the chemostat. In the response control experiment, samples were withdrawn 10 min after addition of 1 mM H2O2 (treated culture) or the equivalent water volume (reference culture)."	"treatment_protocol_ch1.1"
"treatment: treated sinusoidal continous magnetic field"	"characteristics_ch1.1"
"treatment duration: 15 h"	"characteristics_ch1.2"
"growth condition: steady-state continuous cultivation (dilution rate of 0.4 h-1), aerobic"	"characteristics_ch1.3"
"medium: defined mineral medium with 1 g/l glucose"	"characteristics_ch1.4"
"Escherichia coli K-12 strain MG1655 was cultivated aerobically (~0.5 l/min air supply) in a defined mineral medium containing 1 g/l glucose in continous cultures growing in a chemostat (dilution rate = 0.4 h-1, working volume = 100 ml)."	"growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"E_coli_treated_sinusoidal_cont_15h_rep1"	"source_name_ch1.1"
"Samples of magnetic field-treated or sham-treated cells were withdrawn as fast as possible from the bioreactor (not later than 3 min) after the specified treatement period. Samples taken during intermittent exposure were withdrawn in the 4 min exposure break. Unexposed samples were withdrawn as fast as possible during steady-state conditions in the chemostat. In the response control experiment, samples were withdrawn 10 min after addition of 1 mM H2O2 (treated culture) or the equivalent water volume (reference culture)."	"treatment_protocol_ch1.1"