GSE35100-GSM862035-GPL3154-PMID:22684885.tsv
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"strain: DH5a" "characteristics_ch1.1"
"genotype: wild type IrrE" "characteristics_ch1.2"
"Data were analyzed using GeneChip Operating Software Version 1.4 (Affymetrix)" "data_processing.1"
"Total RNA was isolated from the cells stored in liquid nitrogen using TRIzol Reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) and RNeasy MinElute Cleanup Kits (Qiagen, Valencia, CA, USA)" "extract_protocol_ch1.1"
"Cells were grown at 37°C in Luria-Bertani (LB) medium supplemented with 50 μg/ml ampicillin overnight, then inoculated into LB medium supplemented with 0.095% (v/v) furfural and 50 μg/ml ampicillin at an initial OD600 value of 0.02, and grown at 37°C." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"Cells in log phase under furfural stress" "source_name_ch1.1"
"Wild-type IrrE, biological rep1" "title.1"
"The cells were centrifuged when they reached the log phase, and then rapidly chilled and stored in liquid nitrogen until used for the DNA microarray assay" "treatment_protocol_ch1.1"
"strain: DH5a" "characteristics_ch1.1"
"genotype: wild type IrrE" "characteristics_ch1.2"
"Cells were grown at 37°C in Luria-Bertani (LB) medium supplemented with 50 μg/ml ampicillin overnight, then inoculated into LB medium supplemented with 0.095% (v/v) furfural and 50 μg/ml ampicillin at an initial OD600 value of 0.02, and grown at 37°C." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"Cells in log phase under furfural stress" "source_name_ch1.1"
"The cells were centrifuged when they reached the log phase, and then rapidly chilled and stored in liquid nitrogen until used for the DNA microarray assay" "treatment_protocol_ch1.1"