GSE35100-GSM862034-GPL3154-PMID:22684885.tsv
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"strain: DH5a" "characteristics_ch1.1"
"genotype: furfural-tolerant mutant F1-37 of IrrE" "characteristics_ch1.2"
"Data were analyzed using GeneChip Operating Software Version 1.4 (Affymetrix)" "data_processing.1"
"Total RNA was isolated from the cells stored in liquid nitrogen using TRIzol Reagent (Invitrogen Life Technologies, Carlsbad, CA, USA) and RNeasy MinElute Cleanup Kits (Qiagen, Valencia, CA, USA)" "extract_protocol_ch1.1"
"Cells were grown at 37°C in Luria-Bertani (LB) medium supplemented with 50 μg/ml ampicillin overnight, then inoculated into LB medium supplemented with 0.095% (v/v) furfural and 50 μg/ml ampicillin at an initial OD600 value of 0.02, and grown at 37°C." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"Cells in log phase under furfural stress" "source_name_ch1.1"
"Furfural-tolerant IrrE mutant F1-37, biological rep3" "title.1"
"The cells were centrifuged when they reached the log phase, and then rapidly chilled and stored in liquid nitrogen until used for the DNA microarray assay" "treatment_protocol_ch1.1"
"strain: DH5a" "characteristics_ch1.1"
"genotype: furfural-tolerant mutant F1-37 of IrrE" "characteristics_ch1.2"
"Cells were grown at 37°C in Luria-Bertani (LB) medium supplemented with 50 μg/ml ampicillin overnight, then inoculated into LB medium supplemented with 0.095% (v/v) furfural and 50 μg/ml ampicillin at an initial OD600 value of 0.02, and grown at 37°C." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"Cells in log phase under furfural stress" "source_name_ch1.1"
"The cells were centrifuged when they reached the log phase, and then rapidly chilled and stored in liquid nitrogen until used for the DNA microarray assay" "treatment_protocol_ch1.1"