GSE33021-GSM818489-GPL14751-PMID:.tsv 1.96 KB
"genotype: Wild Type"	"characteristics_ch1.1"
"phenotype: normal"	"characteristics_ch1.2"
"accession: U00006"	"characteristics_ch1.3"
"hybridization buffer (formamide content): 15% formamide"	"characteristics_ch1.4"
"hybridization buffer (total rna): 5 ng"	"characteristics_ch1.5"
"Raw data (.pair files) was analyzed using Matlab (The MathWorks, Natick, MA).  For each probe, the average intensity of three replicates were calculated after the elimination of outliers based on a standard deviation test. The average of control (Nonsense) probes was subtracted from all averages to obtain background-corrected results."	"data_processing.1"
"Genomic DNA was extracted using Ultra Clean Soil DNA (MoBio Laboratories, Solana Beach, CA)."	"extract_protocol_ch1.1"
"E. coli was grown to mid-log phase (O.D.600nm 0.3-0.4) in Lennox LB broth (Becton Dickinson, Franklin Lakes, NJ) at 37°C."	"growth_protocol_ch1.1"
"R. sphaeroides was phototrophically grown to mid-log phase (100-150 kletts) in Sistrom's minimal medium (Sistrom, W. 1960. Journal of General Microbiology. 22:778-785) amended with 33.9 mM succinate ."	"growth_protocol_ch1.2"
"Escherichia coli K-12"	"organism_ch1.1"
"Escherichia coli K12 wild-type"	"source_name_ch1.1"
"Eco 5ng - 15%"	"title.1"
"genotype: Wild Type"	"characteristics_ch1.1"
"phenotype: normal"	"characteristics_ch1.2"
"accession: U00006"	"characteristics_ch1.3"
"hybridization buffer (formamide content): 15% formamide"	"characteristics_ch1.4"
"hybridization buffer (total rna): 5 ng"	"characteristics_ch1.5"
"E. coli was grown to mid-log phase (O.D.600nm 0.3-0.4) in Lennox LB broth (Becton Dickinson, Franklin Lakes, NJ) at 37°C."	"growth_protocol_ch1.1"
"R. sphaeroides was phototrophically grown to mid-log phase (100-150 kletts) in Sistrom's minimal medium (Sistrom, W. 1960. Journal of General Microbiology. 22:778-785) amended with 33.9 mM succinate ."	"growth_protocol_ch1.2"
"Escherichia coli K-12"	"organism_ch1.1"
"Escherichia coli K12 wild-type"	"source_name_ch1.1"