GSE32561-GSM807127-GPL14649-PMID:22038610.tsv 2.02 KB
"substrain: DH1"	"characteristics_ch1.1"
"genotype: ∆fadE with overexpression of 'tesA and MlfabH via an IPTG-inudcible promoter"	"characteristics_ch1.2"
"phenotype: small improvement in methyl ketone production"	"characteristics_ch1.3"
"The raw data (.pair file) was subjected to RMA (Robust Multi-Array Analysis; Irizarry et al. Biostatistics 4(2):249), quantile normalization (Bolstad et al. Bioinformatics 19(2):185), and background correction as implemented in the NimbleScan software package, version 2.4.27 (Roche NimbleGen, Inc.)."	"data_processing.1"
"Total RNA was extracted using the RNeasy Mini kit (Qiagen Inc., Valencia, CA, USA) and the RNAprotect reagent (Qiagen) and DNA was removed by on-column DNase digestion with the RNase-Free DNase set (Qiagen). RNA concentration was determined on a Nanodrop ND-1000 (Thermo Scientific) and RNA quality was determined by analysis with an Agilent 2100 bioanalyzer."	"extract_protocol_ch1.1"
"Escherichia coli DH1 ∆fadE control and test strains were seeded with OD600nm 0.03 overnight cultures into 15ml tryptic soy broth media and grown at 37°C with 200rpm agitation."	"growth_protocol_ch1.1"
"Escherichia coli K-12"	"organism_ch1.1"
"EGS212"	"source_name_ch1.1"
"EGS212_MlfabH_rep_3"	"title.1"
"After 6 hours of growth both control and test strains were induced with 0.5mM IPTG and harvested 2 hours post-induction."	"treatment_protocol_ch1.1"
"substrain: DH1"	"characteristics_ch1.1"
"genotype: ∆fadE with overexpression of 'tesA and MlfabH via an IPTG-inudcible promoter"	"characteristics_ch1.2"
"phenotype: small improvement in methyl ketone production"	"characteristics_ch1.3"
"Escherichia coli DH1 ∆fadE control and test strains were seeded with OD600nm 0.03 overnight cultures into 15ml tryptic soy broth media and grown at 37°C with 200rpm agitation."	"growth_protocol_ch1.1"
"Escherichia coli K-12"	"organism_ch1.1"
"EGS212"	"source_name_ch1.1"
"After 6 hours of growth both control and test strains were induced with 0.5mM IPTG and harvested 2 hours post-induction."	"treatment_protocol_ch1.1"