GSE28320-GSM700471-GPL13359-PMID:.tsv
3.46 KB
"transformation: pSigma70-LB" "characteristics_ch1.1"
"treatment: 42 C" "characteristics_ch1.2"
"genotype: 285c rpoD mutation" "characteristics_ch1.3"
"transformation: empty vector" "characteristics_ch2.1"
"treatment: 30 C" "characteristics_ch2.2"
"genotype: 285c rpoD mutation" "characteristics_ch2.3"
"Data were normalized using LOWESS (MIDAS) and analyzed for statistical significance using the Rank Product method in the MultiExperimentViewer" "data_processing.1"
"The Zymo Fungal/Bacterial RNA extraction kit was used to extract RNA from all samples. In-column DNaseI treatment was used to eliminate genomic DNA carryover as described by the manufacturer." "extract_protocol_ch1.1"
"The Zymo Fungal/Bacterial RNA extraction kit was used to extract RNA from all samples. In-column DNaseI treatment was used to eliminate genomic DNA carryover as described by the manufacturer." "extract_protocol_ch2.1"
"All strains were grown in M9 minimal media supplemented with 25 ug/ml streptomycin and 0.52 mM arginine. Samples were grown to OD = 0.5 @ 30 C; experimental samples were then transferred to a shaking water bath @ 42 C." "growth_protocol_ch1.1"
"All strains were grown in M9 minimal media supplemented with 25 ug/ml streptomycin and 0.52 mM arginine. Samples were grown to OD = 0.5 @ 30 C; experimental samples were then transferred to a shaking water bath @ 42 C." "growth_protocol_ch2.1"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli" "organism_ch2.1"
"Sigma70 LB" "source_name_ch1.1"
"285c/control vector at 30 C 1" "source_name_ch2.1"
"Sigma70 LB-1" "title.1"
"Control samples (285c/empty vector) were harvested via filtration and resuspended in RNALater once they had reached OD = 0.5. Experimental samples were harvested after 1 hour of heat shock at 42 C in an analogous procedure." "treatment_protocol_ch1.1"
"Control samples (285c/empty vector) were harvested via filtration and resuspended in RNALater once they had reached OD = 0.5. Experimental samples were harvested after 1 hour of heat shock at 42 C in an analogous procedure." "treatment_protocol_ch2.1"
"transformation: pSigma70-LB" "characteristics_ch1.1"
"treatment: 42 C" "characteristics_ch1.2"
"genotype: 285c rpoD mutation" "characteristics_ch1.3"
"transformation: empty vector" "characteristics_ch2.1"
"treatment: 30 C" "characteristics_ch2.2"
"genotype: 285c rpoD mutation" "characteristics_ch2.3"
"All strains were grown in M9 minimal media supplemented with 25 ug/ml streptomycin and 0.52 mM arginine. Samples were grown to OD = 0.5 @ 30 C; experimental samples were then transferred to a shaking water bath @ 42 C." "growth_protocol_ch1.1"
"All strains were grown in M9 minimal media supplemented with 25 ug/ml streptomycin and 0.52 mM arginine. Samples were grown to OD = 0.5 @ 30 C; experimental samples were then transferred to a shaking water bath @ 42 C." "growth_protocol_ch2.1"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli" "organism_ch2.1"
"Sigma70 LB" "source_name_ch1.1"
"285c/control vector at 30 C 1" "source_name_ch2.1"
"Control samples (285c/empty vector) were harvested via filtration and resuspended in RNALater once they had reached OD = 0.5. Experimental samples were harvested after 1 hour of heat shock at 42 C in an analogous procedure." "treatment_protocol_ch1.1"
"Control samples (285c/empty vector) were harvested via filtration and resuspended in RNALater once they had reached OD = 0.5. Experimental samples were harvested after 1 hour of heat shock at 42 C in an analogous procedure." "treatment_protocol_ch2.1"