"strain: AW1.7"	"characteristics_ch1.1"
"heat sensitivity: resistant"	"characteristics_ch1.2"
"treatment: 37°C"	"characteristics_ch1.3"
"strain: GGG10"	"characteristics_ch2.1"
"heat sensitivity: sensitive"	"characteristics_ch2.2"
"treatment: 37°C"	"characteristics_ch2.3"
"Raw data were normalized and log2 transformed with the Acuity 4.0 software package, and data with low fluorescence intensities were eliminated. Genes were considered to be differentially expressed if the absolute values of log2 mean expression ratios were ≥ 1."	"data_processing.1"
""	"data_processing.2"
"The data did not undergo normalization across samples."	"data_processing.3"
"For RNA isolation, 0.5 mL of the cultures were mixed with 1 mL RNA protect (Qiagen, Ontario, Canada) and RNA was isolated using the RNeasy Mini kit (Qiagen). RNA was quantified with a Nanodrop Spectrophotometer (Thermo scientific, Wilmington, USA) and RNA quality was determined with a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, USA). RNA samples (approximately 30 µg) were treated with 1U RNase free DNase I (Ambion, Ontario, Canada) at 37°C for 30 min and 1U RNase inhibitor (Ambion) was added prior to storage at 4°C. RNA was reverse-transcribed using the Array 900MPX Expression Array Detection Kit (Genisphere, Hatfield, USA) according to the instructions of the manufacturer. The cDNA was purified using the MinElute PCR Purification Kit (Quiagen), and the purified DNA was used for microarray and qPCR experiments."	"extract_protocol_ch1.1"
"For RNA isolation, 0.5 mL of the cultures were mixed with 1 mL RNA protect (Qiagen, Ontario, Canada) and RNA was isolated using the RNeasy Mini kit (Qiagen). RNA was quantified with a Nanodrop Spectrophotometer (Thermo scientific, Wilmington, USA) and RNA quality was determined with a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, USA). RNA samples (approximately 30 µg) were treated with 1U RNase free DNase I (Ambion, Ontario, Canada) at 37°C for 30 min and 1U RNase inhibitor (Ambion) was added prior to storage at 4°C. RNA was reverse-transcribed using the Array 900MPX Expression Array Detection Kit (Genisphere, Hatfield, USA) according to the instructions of the manufacturer. The cDNA was purified using the MinElute PCR Purification Kit (Quiagen), and the purified DNA was used for microarray and qPCR experiments."	"extract_protocol_ch2.1"
"Cultures were inoculated from a single colony, incubated overnight, sub-cultured with a 1% inoculum, and grown to the late exponential growth phase, corresponding to an OD600 nm of 0.6 – 0.7."	"growth_protocol_ch1.1"
"Cultures were inoculated from a single colony, incubated overnight, sub-cultured with a 1% inoculum, and grown to the late exponential growth phase, corresponding to an OD600 nm of 0.6 – 0.7."	"growth_protocol_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"AW1.7, steady-state continuous cultured experimental samples at 37°C"	"source_name_ch1.1"
"GGG10, steady-state continuous cultured experimental samples at 37°C"	"source_name_ch2.1"
"Second biological repeat 37°C"	"title.1"
"RNA was isolated from late exponential cultures, or from late exponential cells heat-shocked by exposure to 50°C for 15 min."	"treatment_protocol_ch1.1"
"RNA was isolated from late exponential cultures, or from late exponential cells heat-shocked by exposure to 50°C for 15 min."	"treatment_protocol_ch2.1"
"strain: AW1.7"	"characteristics_ch1.1"
"heat sensitivity: resistant"	"characteristics_ch1.2"
"treatment: 37°C"	"characteristics_ch1.3"
"strain: GGG10"	"characteristics_ch2.1"
"heat sensitivity: sensitive"	"characteristics_ch2.2"
"treatment: 37°C"	"characteristics_ch2.3"
"Cultures were inoculated from a single colony, incubated overnight, sub-cultured with a 1% inoculum, and grown to the late exponential growth phase, corresponding to an OD600 nm of 0.6 – 0.7."	"growth_protocol_ch1.1"
"Cultures were inoculated from a single colony, incubated overnight, sub-cultured with a 1% inoculum, and grown to the late exponential growth phase, corresponding to an OD600 nm of 0.6 – 0.7."	"growth_protocol_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"AW1.7, steady-state continuous cultured experimental samples at 37°C"	"source_name_ch1.1"
"GGG10, steady-state continuous cultured experimental samples at 37°C"	"source_name_ch2.1"
"RNA was isolated from late exponential cultures, or from late exponential cells heat-shocked by exposure to 50°C for 15 min."	"treatment_protocol_ch1.1"
"RNA was isolated from late exponential cultures, or from late exponential cells heat-shocked by exposure to 50°C for 15 min."	"treatment_protocol_ch2.1"