GSE25745-GSM632643-GPL3154-PMID:21169441.tsv 2.33 KB
"strain: CFT073"	"characteristics_ch1.1"
"growth condition: LB broth alone (control)"	"characteristics_ch1.2"
"The data were analyzed with FlexArray 1.4.1 software program (http://genomequebec.mcgill.ca/FlexArray/). The data were normalized using the Robust Multi-Array Average algorithm (RMA). Comparison between the two data sets was done using the Randomized Variance Model (RVM) with a cut-off of a fold change of 2 (or -2) and a P-value of 0.05."	"data_processing.1"
"RNA extraction was performed according to the manufacturer's (Qiagen) instructions."	"extract_protocol_ch1.1"
"Cultures of E. coli CFT073 were maintained at –80ºC in Luria-Bertani broth with 15% glycerol. Cultures were streaked onto LB agar plates and incubated (37°C, 24 h).  A single colony was inoculated into 150 mL of LB broth (in a 500 mL baffled flask).  Three inoculated flasks contained LB broth alone (controls), and three inoculated flasks were supplemented with cranberry derived PAC extract (100 µg/mL).Bacteria were incubated at 37°C, 5 h, 200 rpm to mid-log growth phase."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"CFT073 in LB, after 5 hours of growth."	"source_name_ch1.1"
"CFT073_LB_rep3"	"title.1"
"One volume of bacterial culture was added to two volumes of RNAprotect Bacteria Reagent (Qiagen) and RNA was extracted using RNeasy mini kit (Qiagen).  All samples were on-column treated with DNase I."	"treatment_protocol_ch1.1"
"strain: CFT073"	"characteristics_ch1.1"
"growth condition: LB broth alone (control)"	"characteristics_ch1.2"
"Cultures of E. coli CFT073 were maintained at –80ºC in Luria-Bertani broth with 15% glycerol. Cultures were streaked onto LB agar plates and incubated (37°C, 24 h).  A single colony was inoculated into 150 mL of LB broth (in a 500 mL baffled flask).  Three inoculated flasks contained LB broth alone (controls), and three inoculated flasks were supplemented with cranberry derived PAC extract (100 µg/mL).Bacteria were incubated at 37°C, 5 h, 200 rpm to mid-log growth phase."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"CFT073 in LB, after 5 hours of growth."	"source_name_ch1.1"
"One volume of bacterial culture was added to two volumes of RNAprotect Bacteria Reagent (Qiagen) and RNA was extracted using RNeasy mini kit (Qiagen).  All samples were on-column treated with DNase I."	"treatment_protocol_ch1.1"