GSE25106-GSM617020-GPL6702-PMID:21422176.tsv
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"genotype/variation: flhD" "characteristics_ch1.1"
"RMA with quartile normalization according to method of Irizarry et al, 2003. The raw .cel files were processed in RMA using Bioconductor R package: affy Version:1.8.1 Date:2005-09-03, with the option Filter.genes containing a subset of probesets corresponding to MG1655 genes and intergenic regions listed in the matrix data. Replicates were averaged for ratio calculations." "data_processing.1"
"Cultures were diluted into DNA-RNA Protect (Sierra Diagnostics) to inhibit RNA degradation and the RNA was purified using RNeasy minkits with the optional DNase treatment (Qiagen)." "extract_protocol_ch1.1"
"Cells were grown at 37oC without shaking in 18 mm test tubes containing 5 ml of the MOPS-based culture medium designed by Neidhardt. Mouse cecal mucus was prepared from streptomycin-treated CD-1 mice (not colonized with E. coli). Cultures were grown to A600 = 0.4 in MOPS medium containing glucose or mannose (0.2%) and also in 10 mg/ml of lyophilized mucus at A600=0.2 (P1) or 0.5 (P2)." "growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655" "organism_ch1.1"
"E. coli flhD, A600=0.4 on mannose" "source_name_ch1.1"
"flhD-mannose" "title.1"
"genotype/variation: flhD" "characteristics_ch1.1"
"Cells were grown at 37oC without shaking in 18 mm test tubes containing 5 ml of the MOPS-based culture medium designed by Neidhardt. Mouse cecal mucus was prepared from streptomycin-treated CD-1 mice (not colonized with E. coli). Cultures were grown to A600 = 0.4 in MOPS medium containing glucose or mannose (0.2%) and also in 10 mg/ml of lyophilized mucus at A600=0.2 (P1) or 0.5 (P2)." "growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655" "organism_ch1.1"
"E. coli flhD, A600=0.4 on mannose" "source_name_ch1.1"