GSE22060-GSM544430-GPL7001-PMID:21725004.tsv 2.55 KB
"ehec strain: 86-24"	"characteristics_ch1.1"
"ehec strain: 86-24"	"characteristics_ch2.1"
"Signal intensities were averaged among the technical replicates.  Two types of data analysis were performed: to indentify density-dependent changes in gene expression, single averaged normalized signal intensities for each treatment point were compared to the average of corresponding unstressed control signal intensities.  Genes which showed a relative signal log2ratio (SLR) value above 1.0 or below -1.0 were selected for further analysis. "	"data_processing.1"
"RNA was extracted using Trizol (Invitrogen)"	"extract_protocol_ch1.1"
"RNA was extracted using Trizol (Invitrogen)"	"extract_protocol_ch2.1"
"Bacteria were grown in LB broth overnight and subcultured in DMEM to OD600 of 0.4. Bacteria were then spun down, media removed and repalced with DMEM plus 0.15% bile salt mix. "	"growth_protocol_ch1.1"
"Bacteria were grown in LB broth overnight and subcultured in DMEM to OD600 of 0.4. Bacteria were then spun down, media removed and repalced with DMEM. "	"growth_protocol_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"Cy5 labeled EHEC bile salt-stressed in DMEM, 90 minutes"	"source_name_ch1.1"
"Cy3 labeled EHEC grown in DMEM pH 7.4, 90 minutes"	"source_name_ch2.1"
"0.15% Bile Salt Mix Stress, 90 minutes, static, 5% CO2 (EHEC strain: 86-24)"	"title.1"
"Bile salt stress was done in DMEM at pH 7.4 (unbuffered) at 37C, 5% CO2, static incubation for 90 minutes"	"treatment_protocol_ch1.1"
"Control treatment. Bacteria were grown in DMEM pH 7.4, 90 minutes, 37C, 5% CO2, static (unstressed)"	"treatment_protocol_ch2.1"
"ehec strain: 86-24"	"characteristics_ch1.1"
"ehec strain: 86-24"	"characteristics_ch2.1"
"Bacteria were grown in LB broth overnight and subcultured in DMEM to OD600 of 0.4. Bacteria were then spun down, media removed and repalced with DMEM plus 0.15% bile salt mix. "	"growth_protocol_ch1.1"
"Bacteria were grown in LB broth overnight and subcultured in DMEM to OD600 of 0.4. Bacteria were then spun down, media removed and repalced with DMEM. "	"growth_protocol_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"Cy5 labeled EHEC bile salt-stressed in DMEM, 90 minutes"	"source_name_ch1.1"
"Cy3 labeled EHEC grown in DMEM pH 7.4, 90 minutes"	"source_name_ch2.1"
"Bile salt stress was done in DMEM at pH 7.4 (unbuffered) at 37C, 5% CO2, static incubation for 90 minutes"	"treatment_protocol_ch1.1"
"Control treatment. Bacteria were grown in DMEM pH 7.4, 90 minutes, 37C, 5% CO2, static (unstressed)"	"treatment_protocol_ch2.1"