GSE19742-GSM493012-GPL9855-PMID:20478253.tsv
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"growth stage: log phase" "characteristics_ch1.1"
"strain: MG1655 delta lac delta dksA" "characteristics_ch1.2"
"treatment: 0.5mg/ml SHX for 40min" "characteristics_ch1.3"
"growth stage: log phase" "characteristics_ch2.1"
"strain: MG1655 delta lac + MG1655 delta lac delta dksA" "characteristics_ch2.2"
"treatment: Pooled RNA from mixture of the same amount of wt, wt+SHX, dksA, dksA+SHX RNA samples" "characteristics_ch2.3"
"GenePix Pro (v 6.1) was used for background subtraction and normalization." "data_processing.1"
"RNA was extracted using QIAGEN RNeasy Mini Kit following manufacturer's instructions." "extract_protocol_ch1.1"
"RNA was extracted using QIAGEN RNeasy Mini Kit following manufacturer's instructions." "extract_protocol_ch2.1"
"Cells were grown in M9 medium supplemented with 0.2% glucose and 0.4% casamino acid at 37℃" "growth_protocol_ch1.1"
"Cells were grown in M9 medium supplemented with 0.2% glucose and 0.4% casamino acid at 37℃" "growth_protocol_ch2.1"
"Escherichia coli str. K-12 substr. MG1655" "organism_ch1.1"
"Escherichia coli str. K-12 substr. MG1655" "organism_ch2.1"
"SHX-treated Delta dksA Cells" "source_name_ch1.1"
"pooled reference RNA" "source_name_ch2.1"
"SHX-treated Delta dksA Cells Replicate 2" "title.1"
"At OD600~0.3, serine hydroxamate was added to a final concentration of 0.5mg/ml, cells were collected 40 minutes later." "treatment_protocol_ch1.1"
"At OD600~0.3, serine hydroxamate was added to a final concentration of 0.5mg/ml, cells were collected 40 minutes later." "treatment_protocol_ch2.1"
"growth stage: log phase" "characteristics_ch1.1"
"strain: MG1655 delta lac delta dksA" "characteristics_ch1.2"
"treatment: 0.5mg/ml SHX for 40min" "characteristics_ch1.3"
"growth stage: log phase" "characteristics_ch2.1"
"strain: MG1655 delta lac + MG1655 delta lac delta dksA" "characteristics_ch2.2"
"treatment: Pooled RNA from mixture of the same amount of wt, wt+SHX, dksA, dksA+SHX RNA samples" "characteristics_ch2.3"
"Cells were grown in M9 medium supplemented with 0.2% glucose and 0.4% casamino acid at 37℃" "growth_protocol_ch1.1"
"Cells were grown in M9 medium supplemented with 0.2% glucose and 0.4% casamino acid at 37℃" "growth_protocol_ch2.1"
"Escherichia coli str. K-12 substr. MG1655" "organism_ch1.1"
"Escherichia coli str. K-12 substr. MG1655" "organism_ch2.1"
"SHX-treated Delta dksA Cells" "source_name_ch1.1"
"pooled reference RNA" "source_name_ch2.1"
"At OD600~0.3, serine hydroxamate was added to a final concentration of 0.5mg/ml, cells were collected 40 minutes later." "treatment_protocol_ch1.1"
"At OD600~0.3, serine hydroxamate was added to a final concentration of 0.5mg/ml, cells were collected 40 minutes later." "treatment_protocol_ch2.1"