"strain: MG1655"	"characteristics_ch1.1"
"plasmid: pPROEx-CAT"	"characteristics_ch1.2"
"Microarray suit 5.0 (Affymetrix Inc.) was used to average the scans and process the data, further data analysis was conducted using Affymetrix® Data Mining Tool (DMT 3.0)."	"data_processing.1"
"Fifteen mL fermentation broth was used for each RNA extraction. Harvested cells were rapidly cooled in a -80°C ethanol bath, then centrifuged at 5000 g for seven minutes at 4°C. The supernatant was discarded. The cell pellet was frozen at -80°C. Thawed (on ice) cell pellets were suspended in 150 mL of TE buffer (10 mM Tris-HCl, pH 7.6, 0.1 mM EDTA) containing 0.75 mg/mL lysozyme, and incubated for 15 minutes at room temperature. Total RNA was isolated using RNAqueousTM-Midi Kit (Ambion). The concentration of purified total RNA was determined spectrophotometrically by measuring absorbance at 260 nm. The quality and integrity of the isolated RNA was confirmed by agarose gels (clear 23S and 16S rRNA bands)."	"extract_protocol_ch1.1"
"The strain Escherichia coli MG1655 [pPROEx-CAT] was cultured in a fed-batch fermenter in LB media w/ glucose and a glucose/MgS04 feed at 37 degrees.  Cultures were synchronized to the late log phase, approximately 4.5 hours of fermentation time.  Serine hydrxamate (100mg/L final concentration) was added to the cultures. Samples were taken 1 hour after the beginning the serine hydrxamate addition."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E. coli, 1-h, serine hydroxamate"	"source_name_ch1.1"
"Serine Hydroxamate stressed, replicate #3"	"title.1"
"Serine hydrxamate (100 mg/L final concentration) was added to the cultures in the late-exponential phase at ~11 OD (~5.5 g g dry cell weight per liter).  Samples for DNA microarray analysis were taken 1 hour after the beginning the serine hydrxamate addition (13.2 OD, ~6.6 g dry cell weight per liter, growth rate 0.15 1/h)."	"treatment_protocol_ch1.1"
"strain: MG1655"	"characteristics_ch1.1"
"plasmid: pPROEx-CAT"	"characteristics_ch1.2"
"The strain Escherichia coli MG1655 [pPROEx-CAT] was cultured in a fed-batch fermenter in LB media w/ glucose and a glucose/MgS04 feed at 37 degrees.  Cultures were synchronized to the late log phase, approximately 4.5 hours of fermentation time.  Serine hydrxamate (100mg/L final concentration) was added to the cultures. Samples were taken 1 hour after the beginning the serine hydrxamate addition."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E. coli, 1-h, serine hydroxamate"	"source_name_ch1.1"
"Serine hydrxamate (100 mg/L final concentration) was added to the cultures in the late-exponential phase at ~11 OD (~5.5 g g dry cell weight per liter).  Samples for DNA microarray analysis were taken 1 hour after the beginning the serine hydrxamate addition (13.2 OD, ~6.6 g dry cell weight per liter, growth rate 0.15 1/h)."	"treatment_protocol_ch1.1"