GSE17074-GSM427210-GPL8850-PMID:.tsv 1.73 KB
"strain: DH5α"	"characteristics_ch1.1"
"strain: DH5α"	"characteristics_ch2.1"
"The raw data were normalized using a space and intensity-dependant LOWESS program. Data from faint spots were removed, in which the intensity was lower than the average intensity plus 2 standard deviations of the negative controls on the array. For each experimental and control sample, amplification and hybridization were performed by a dye-swap strategy in two independent experiments. Differentially expressed genes were identified with at least 2 fold changes and q<0.001 criteria using SAM software ."	"data_processing.1"
"Total RNA was isolated from E.coli cells using NucleoSpin® RNA II kit according to the manufacturers’ instructions (Macherey Nagel, Duren, Germany). RNA samples were examined in a 1.5% denaturing agarose gel, quantitated by absorbance at 260 nm and stored until further use for microarray and RT-PCR verification."	"extract_protocol_ch1.1"
"Total RNA was isolated from E.coli cells using NucleoSpin® RNA II kit according to the manufacturers’ instructions (Macherey Nagel, Duren, Germany). RNA samples were examined in a 1.5% denaturing agarose gel, quantitated by absorbance at 260 nm and stored until further use for microarray and RT-PCR verification"	"extract_protocol_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"E.coli, 43°C, 60min"	"source_name_ch1.1"
"E.coli, 43°C, 60min"	"source_name_ch2.1"
"E. coli heat shock gene expression data using PAOD method (replicate-2)"	"title.1"
"strain: DH5α"	"characteristics_ch1.1"
"strain: DH5α"	"characteristics_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"E.coli, 43°C, 60min"	"source_name_ch1.1"
"E.coli, 43°C, 60min"	"source_name_ch2.1"