GSE16973-GSM424875-GPL8811-PMID:20118358.tsv 2.03 KB
"strain: E. coli DH1"	"characteristics_ch1.1"
"treatment: 0.8% n-butanol was added at time 0"	"characteristics_ch1.2"
"time: 80 min"	"characteristics_ch1.3"
"strain: E. coli DH1"	"characteristics_ch2.1"
"Feature fluorescence intensities [F532 media-B532 and F635media-B635] were extracted using GenePix Pro 6.0.  Local hybridization and intensity-dependent artifacts were removed with Lowess normalization using Standardization and Normalization of Microarray Data (SNOMAD, http://pevsnerlab.kennedykrieger.org/snomadinput.html).  The three replicate spots for each gene were averaged and variance was calculated."	"data_processing.1"
"Biomass was snap frozen in liquid nitrogen and stored at -80 C prior to extraction with Qiagen RNeasy Midi kits.  RNA integrity was verified with a 2100 Bioanalyzer (Agilent)."	"extract_protocol_ch1.1"
"Biomass was snap frozen in liquid nitrogen and stored at -80 C prior to extraction with Qiagen RNeasy Midi kits.  RNA integrity was verified with a 2100 Bioanalyzer (Agilent)."	"extract_protocol_ch2.1"
"Individual clones of E. coli DH1 were grown in M9 MOPS media in 300 mL media in 1 L baffled flasks"	"growth_protocol_ch1.1"
"Individual clones of E. coli DH1 were grown in M9 MOPS media in 300 mL media in 1 L baffled flasks"	"growth_protocol_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"80 min, 0.8% butanol"	"source_name_ch1.1"
"80 min, unstressed"	"source_name_ch2.1"
"80min-3"	"title.1"
"strain: E. coli DH1"	"characteristics_ch1.1"
"treatment: 0.8% n-butanol was added at time 0"	"characteristics_ch1.2"
"time: 80 min"	"characteristics_ch1.3"
"strain: E. coli DH1"	"characteristics_ch2.1"
"Individual clones of E. coli DH1 were grown in M9 MOPS media in 300 mL media in 1 L baffled flasks"	"growth_protocol_ch1.1"
"Individual clones of E. coli DH1 were grown in M9 MOPS media in 300 mL media in 1 L baffled flasks"	"growth_protocol_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"80 min, 0.8% butanol"	"source_name_ch1.1"
"80 min, unstressed"	"source_name_ch2.1"