GSE13698-GSM344441-GPL199-GPL3154-PMID:19919618.tsv
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"RNA extracted from cells of TG1/pMMB206-TOM-Green/pBS(Kan)-EchA F108L/I219L/C248I after contacting for 2 h with cis-DCE (1 mM) in Tris-HNO3 buffer with succinate (5 mM), IPTG (0.5 mM) at 37oC" "characteristics_ch1.1"
"MAS 5.0 Expression Analysis Default Setting" "data_processing.1"
"To lyse the cells, 1.0 mL RLT buffer (Qiagen, Inc., Valencia, CA) and 0.2 mL 0.1 mm zirconia/silica beads (Biospec) were added to the frozen bead beater tubes containing the cell pellets. The tubes were closed tightly and beat for 50 seconds at the maximum speed in a mini bead beater (cat. no. 3110BX, Biospec). The total RNA was isolated by following the protocol of the RNeasy Mini Kit (Qiagen) including an on-column DNase digestion with RNase-free DNase I (Qiagen). " "extract_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"TG1/pMMB206-TOM-Green/pBS(Kan)-EchA F108L/I219L/C248I, cis-DCE" "source_name_ch1.1"
"TG1/pMMB206-TOM-Green/pBS(Kan)-EchA F108L/I219L/C248I, cis-DCE" "title.1"
"RNA extracted from cells of TG1/pMMB206-TOM-Green/pBS(Kan)-EchA F108L/I219L/C248I after contacting for 2 h with cis-DCE (1 mM) in Tris-HNO3 buffer with succinate (5 mM), IPTG (0.5 mM) at 37oC" "characteristics_ch1.1"
"Escherichia coli" "organism_ch1.1"
"TG1/pMMB206-TOM-Green/pBS(Kan)-EchA F108L/I219L/C248I, cis-DCE" "source_name_ch1.1"