GSE13698-GSM344439-GPL199-GPL3154-PMID:19919618.tsv 1.24 KB
"RNA extracted from cells of TG1/pBS(Kan)-TOM-Green/pMMB277-IsoILR1-GSHI after contacting for 2 h with cis-DCE (1 mM) in Tris-HNO3 buffer with succinate (5 mM), IPTG (0.5 mM) at 37oC"	"characteristics_ch1.1"
"MAS 5.0 Expression Analysis Default Setting"	"data_processing.1"
"To lyse the cells, 1.0 mL RLT buffer (Qiagen, Inc., Valencia, CA) and 0.2 mL 0.1 mm zirconia/silica beads (Biospec) were added to the frozen bead beater tubes containing the cell pellets. The tubes were closed tightly and beat for 50 seconds at the maximum speed in a mini bead beater (cat. no. 3110BX, Biospec). The total RNA was isolated by following the protocol of the RNeasy Mini Kit (Qiagen) including an on-column DNase digestion with RNase-free DNase I (Qiagen). "	"extract_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"TG1/pBS(Kan)-TOM-Green/pMMB277-IsoILR1-GSHI, cis-DCE"	"source_name_ch1.1"
"TG1/pBS(Kan)-TOM-Green/pMMB277-IsoILR1-GSHI, cis-DCE"	"title.1"
"RNA extracted from cells of TG1/pBS(Kan)-TOM-Green/pMMB277-IsoILR1-GSHI after contacting for 2 h with cis-DCE (1 mM) in Tris-HNO3 buffer with succinate (5 mM), IPTG (0.5 mM) at 37oC"	"characteristics_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"TG1/pBS(Kan)-TOM-Green/pMMB277-IsoILR1-GSHI, cis-DCE"	"source_name_ch1.1"