GSE12797-GSM321238-GPL199-PMID:18843507.tsv 1.27 KB
"MG1655 rpoS mutants"	"characteristics_ch1.1"
"GCRMA normalized"	"data_processing.1"
"Acidic Hot Phenol"	"extract_protocol_ch1.1"
"Cells were grown in M63 glucose (0.2%) minimal media at 37oC at 200 rpm. Overnight cultures were inoculated with single, independent colonies in triplicate, incubated aerobically overnight at 37oC, and sub-cultured to a starting OD600 of 0.0001. Cultures were harvested at an OD600 = 0.3 after being maintained in exponential phase for at least eight generations."	"growth_protocol_ch1.1"
"Escherichia coli K-12"	"organism_ch1.1"
"E. coli K12 grown in M63 glucose (0.2%) minimal media, sampled in exponential phase OD600=0.3"	"source_name_ch1.1"
"Exp-rpoS-Rep3"	"title.1"
"MG1655 rpoS mutants"	"characteristics_ch1.1"
"Cells were grown in M63 glucose (0.2%) minimal media at 37oC at 200 rpm. Overnight cultures were inoculated with single, independent colonies in triplicate, incubated aerobically overnight at 37oC, and sub-cultured to a starting OD600 of 0.0001. Cultures were harvested at an OD600 = 0.3 after being maintained in exponential phase for at least eight generations."	"growth_protocol_ch1.1"
"Escherichia coli K-12"	"organism_ch1.1"
"E. coli K12 grown in M63 glucose (0.2%) minimal media, sampled in exponential phase OD600=0.3"	"source_name_ch1.1"