GSE11894-GSM300523-GPL534-PMID:19377097.tsv
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"Steady-state continuously-cultured MG1655 in the presence of adequate Zn" "characteristics_ch1.1"
"Steady-state continuously-cultured MG1655 in the total absence of Zn" "characteristics_ch2.1"
"Spots automatically flagged as bad, negative or poor in the Imagene software were removed before the statistical analysis was carried out in GeneSight. The mean values from each channel were log2 transformed and normalised using the Lowess method to remove intensity-dependent effects in the log2(ratios) values. The Cy3/Cy5 or Cy5/Cy3 (test/reference) fluorescent ratios were calculated from the normalized values." "data_processing.1"
"After 50 hours, samples (10 ml) were harvested directly into RNA Protect (Qiagen) to stabilize RNA, and total RNA was purified using Qiagen’s RNeasy Mini kit as recommended by the suppliers. RNA was quantified using a BioPhotometer (Eppendorf)." "extract_protocol_ch1.1"
"After 50 hours, samples (10 ml) were harvested directly into RNA Protect (Qiagen) to stabilize RNA, and total RNA was purified using Qiagen’s RNeasy Mini kit as recommended by the suppliers. RNA was quantified using a BioPhotometer (Eppendorf)." "extract_protocol_ch2.1"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli" "organism_ch2.1"
"Steady-state continuously-cultured control samples in the presence of adequate Zn" "source_name_ch1.1"
"Steady-state continuously-cultured experiment samples in the total absence of Zn" "source_name_ch2.1"
"Transcriptional profiling of Escherichia coli cultured under severe zinc limitation Chemostat 3A" "title.1"
"Steady-state continuously-cultured MG1655 in the presence of adequate Zn" "characteristics_ch1.1"
"Steady-state continuously-cultured MG1655 in the total absence of Zn" "characteristics_ch2.1"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli" "organism_ch2.1"
"Steady-state continuously-cultured control samples in the presence of adequate Zn" "source_name_ch1.1"
"Steady-state continuously-cultured experiment samples in the total absence of Zn" "source_name_ch2.1"