GSE112421-GSM3069900-GPL13359-PMID:.tsv
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"strain: E058" "characteristics_ch1.1"
"genotype/varation: mutant" "characteristics_ch1.2"
"condition: in vivo" "characteristics_ch1.3"
"Data were extracted with Feature Extraction software 10.7 (Agilent technologies, Santa Clara, CA, US). Raw data were normalized by Quantile algorithm, GeneSpring Software 11.0 (Agilent technologies, Santa Clara, CA, US)." "data_processing.1"
"Total RNA from in vivo samples of APEC E058 and E058ΔrstAB was extracted and purified using RNeasy mini kit (Cat#74106, QIAGEN, GmBH, Germany) following the manufacturer’s instructions." "extract_protocol_ch1.1"
"To prepare the in vivo samples, the bacteria was harvested from cardiac blood in 1-day-old chickens at 5 hours post infection." "growth_protocol_ch1.1"
"Escherichia coli APEC O2" "organism_ch1.1"
"bacteria harvested from blood of chickens" "source_name_ch1.1"
"E058ΔrstAB in vivo, biological rep3" "title.1"
"The in vivo bacterial samples were harvested with two-step centrifugation. We first centrifuged the collected anticoagulated blood samples at low speed to remove the abundant red and white blood cells and collected the upper serum layer. We then used high-speed centrifugation to precipitate the bacteria from the serum." "treatment_protocol_ch1.1"
"strain: E058" "characteristics_ch1.1"
"genotype/varation: mutant" "characteristics_ch1.2"
"condition: in vivo" "characteristics_ch1.3"
"To prepare the in vivo samples, the bacteria was harvested from cardiac blood in 1-day-old chickens at 5 hours post infection." "growth_protocol_ch1.1"
"Escherichia coli APEC O2" "organism_ch1.1"
"bacteria harvested from blood of chickens" "source_name_ch1.1"
"The in vivo bacterial samples were harvested with two-step centrifugation. We first centrifuged the collected anticoagulated blood samples at low speed to remove the abundant red and white blood cells and collected the upper serum layer. We then used high-speed centrifugation to precipitate the bacteria from the serum." "treatment_protocol_ch1.1"