"E. coli strain B178 (Georgopoulos et al. (1973) J Mol Biol 76: 45-60) in which the endogenous groES and groEL genes have been deleted and that is maintained alive by the pOFX-tac1-derived plasmid carrying an IPTG-inducible operon with cDNA encoding human Hsp10 and the mature part of human wild type Hsp60 (Hansen et al., 2002). These cells were subsequently transformed by electroporation with a second plasmid, derivative of pBAD/hisA (Invitrogen), that contained an operon with Hsp10 and wild type Hsp60 under control of the arabinose-inducible BAD promoter. The pBAD/hisA-derived plasmids carry a pBR322 origin of replication and an ampicillin resistance gene and can be stably maintained in the same cell with the p15A-origin/kanamycin resistance gene containing pOFX-tac derivative."	"characteristics_ch1.1"
"MAS5.0"	"data_processing.1"
"Total RNA was purified using the RNAeasy kit (Qiagen)."	"extract_protocol_ch1.1"
"Bacterial cells were grown in dYT medium (Miller, JH (1972) Experiments in Molecular Genetics (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY) supplemented with kanamycin (10 mg/L), ampicillin (100 mg/L) and IPTG (0.1mM) at 30°C in a shaking water bath. At a turbidity (measured at OD536) of approximately 0.1 bacteria were harvested by centrifugation at ambient temperature and resuspended in fresh dYT medium containing antibiotics and 0.2% arabinose. Growth was continued at 30°C and 10 hours after inducer shift 3ml sample were withdrawn, treated with RNAPROTECT (Qiagen, Ballerup), harvested by centrifugation and frozen."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E. coli B178 groESL(-) expressing human Hsp60 and Hsp10"	"source_name_ch1.1"
"E.coli/Hsp60-wt/wt(-/+)"	"title.1"
"E. coli strain B178 (Georgopoulos et al. (1973) J Mol Biol 76: 45-60) in which the endogenous groES and groEL genes have been deleted and that is maintained alive by the pOFX-tac1-derived plasmid carrying an IPTG-inducible operon with cDNA encoding human Hsp10 and the mature part of human wild type Hsp60 (Hansen et al., 2002). These cells were subsequently transformed by electroporation with a second plasmid, derivative of pBAD/hisA (Invitrogen), that contained an operon with Hsp10 and wild type Hsp60 under control of the arabinose-inducible BAD promoter. The pBAD/hisA-derived plasmids carry a pBR322 origin of replication and an ampicillin resistance gene and can be stably maintained in the same cell with the p15A-origin/kanamycin resistance gene containing pOFX-tac derivative."	"characteristics_ch1.1"
"Bacterial cells were grown in dYT medium (Miller, JH (1972) Experiments in Molecular Genetics (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY) supplemented with kanamycin (10 mg/L), ampicillin (100 mg/L) and IPTG (0.1mM) at 30°C in a shaking water bath. At a turbidity (measured at OD536) of approximately 0.1 bacteria were harvested by centrifugation at ambient temperature and resuspended in fresh dYT medium containing antibiotics and 0.2% arabinose. Growth was continued at 30°C and 10 hours after inducer shift 3ml sample were withdrawn, treated with RNAPROTECT (Qiagen, Ballerup), harvested by centrifugation and frozen."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"E. coli B178 groESL(-) expressing human Hsp60 and Hsp10"	"source_name_ch1.1"