"HNCE4 is an isogenic mutant derived from drug sensitive AG102MB (marR1, AcrB::kan). It contains an additional mutation at the emrAB locus (marR1, acrB::kan, emrAB::cat) resulting in a multidrug efflux negative phenotype."	"characteristics_ch1.1"
"The parent strain, AG102, was previously derived from AG100 (K-12).  AG102 is drug hyper-resistant due to a mutation in marR (marR1) which increases expression of MarA, a global regulator, which in turn results in overexpression of the AcrAB-TolC multidrug efflux system."	"characteristics_ch2.1"
"The analyzed data were imported to ArrayTrack (NCTR Center for Toxicoinformatics;http://www.fda.gov/nctr/science/centers/toxicoinformatics/ArrayTrack/arraytrack_webaccess.htm). The raw data were normalized by using median channel (intensity) scaling with no background subtraction."	"data_processing.1"
"Total RNA was extracted using Qiagen RNeasy kit."	"extract_protocol_ch1.1"
"Total RNA was extracted using Qiagen RNeasy kit."	"extract_protocol_ch2.1"
"Cultures were grown to mid-log phase in Luria-Bertani (Miller) broth medium at 37C.  Cells were cultured with 25 ug/ml chloramphenicol to maintain selection. However, this genomic mutation is stable and RNA was prepared from non-selectively grown cultures inoculated with overnight stocks (grown with selection) to eliminate any unrelated effects of exogenous drugs on gene expression."	"growth_protocol_ch1.1"
"Cultures were grown to mid-log phase in Luria-Bertani (Miller) broth medium at 37C."	"growth_protocol_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"AcrB- and EmrAB-Negative, Multidrug Efflux Mutant Strain"	"source_name_ch1.1"
"Efflux Mutant Parent Strain"	"source_name_ch2.1"
"E. coli AcrB and EmrAB Multidrug Efflux Double Mutant"	"title.1"
"HNCE4 is an isogenic mutant derived from drug sensitive AG102MB (marR1, AcrB::kan). It contains an additional mutation at the emrAB locus (marR1, acrB::kan, emrAB::cat) resulting in a multidrug efflux negative phenotype."	"characteristics_ch1.1"
"The parent strain, AG102, was previously derived from AG100 (K-12).  AG102 is drug hyper-resistant due to a mutation in marR (marR1) which increases expression of MarA, a global regulator, which in turn results in overexpression of the AcrAB-TolC multidrug efflux system."	"characteristics_ch2.1"
"Cultures were grown to mid-log phase in Luria-Bertani (Miller) broth medium at 37C.  Cells were cultured with 25 ug/ml chloramphenicol to maintain selection. However, this genomic mutation is stable and RNA was prepared from non-selectively grown cultures inoculated with overnight stocks (grown with selection) to eliminate any unrelated effects of exogenous drugs on gene expression."	"growth_protocol_ch1.1"
"Cultures were grown to mid-log phase in Luria-Bertani (Miller) broth medium at 37C."	"growth_protocol_ch2.1"
"Escherichia coli"	"organism_ch1.1"
"Escherichia coli"	"organism_ch2.1"
"AcrB- and EmrAB-Negative, Multidrug Efflux Mutant Strain"	"source_name_ch1.1"
"Efflux Mutant Parent Strain"	"source_name_ch2.1"