GSE94984-GSM2493797-GPL23073-PMID_28245801.tsv 6.22 KB
SRR5266619	GSE94984	GSM2493797	GPL23073	PMID_28245801	EHEC in LB Experiment 2 [RNA-Seq]	Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP	GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933)	data_processing	Illumina fastq files were submitted to the galaxy pipeline => grooming => mapping to NC_002655 using bowtie2 (default but seed 19 nt & zero mismatches within seed) PGCGROWTHCONDITIONS
SRR5266619	GSE94984	GSM2493797	GPL23073	PMID_28245801	EHEC in LB Experiment 2 [RNA-Seq]	Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP	GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933)	data_processing	Genome_build: NC_002655 PGCGROWTHCONDITIONS
SRR5266619	GSE94984	GSM2493797	GPL23073	PMID_28245801	EHEC in LB Experiment 2 [RNA-Seq]	Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP	GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933)	data_processing	Supplementary_files_format_and_content: Reads, RPKM, and RCV mapped to NC_002655 (RCV = Ribosomal Coverage Value = RPKM-footprint over RPKM-transcription) PGCGROWTHCONDITIONS
SRR5266619	GSE94984	GSM2493797	GPL23073	PMID_28245801	EHEC in LB Experiment 2 [RNA-Seq]	Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP	GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933)	extract_protocol	Trizol for RNAseq, gradient centrifugation => RNase digestions => Trizol => gel extraction for RIBOseq PGCGROWTHCONDITIONS
SRR5266619	GSE94984	GSM2493797	GPL23073	PMID_28245801	EHEC in LB Experiment 2 [RNA-Seq]	Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP	GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933)	extract_protocol	Truseq Small RNA PGCGROWTHCONDITIONS
SRR5266619	GSE94984	GSM2493797	GPL23073	PMID_28245801	EHEC in LB Experiment 2 [RNA-Seq]	Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP	GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933)	extract_protocol	ligation, rt-PCR, PCR PGCGROWTHCONDITIONS
SRR5266619	GSE94984	GSM2493797	GPL23073	PMID_28245801	EHEC in LB Experiment 2 [RNA-Seq]	Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP	GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933)	growth_protocol	LB medium, 180 rpm shaking, at 37°C, between exponential and stationary phase PGCGROWTHCONDITIONS
SRR5266619	GSE94984	GSM2493797	GPL23073	PMID_28245801	EHEC in LB Experiment 2 [RNA-Seq]	Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP	GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933)	organism	Escherichia coli O157:H7 str. EDL933 PGCGROWTHCONDITIONS
SRR5266619	GSE94984	GSM2493797	GPL23073	PMID_28245801	EHEC in LB Experiment 2 [RNA-Seq]	Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP	GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933)	source_name	biomass collected in the transition between exponential to stationary phase PGCGROWTHCONDITIONS
SRR5266619	GSE94984	GSM2493797	GPL23073	PMID_28245801	EHEC in LB Experiment 2 [RNA-Seq]	Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP	GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933)	library_strategy	RNA-Seq PGCGROWTHCONDITIONS
SRR5266619	GSE94984	GSM2493797	GPL23073	PMID_28245801	EHEC in LB Experiment 2 [RNA-Seq]	Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP	GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933)	characteristics	growth media: LB medium PGCGROWTHCONDITIONS
SRR5266619	GSE94984	GSM2493797	GPL23073	PMID_28245801	EHEC in LB Experiment 2 [RNA-Seq]	Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP	GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933)	growth_protocol	LB medium, 180 rpm shaking, at 37°C, between exponential and stationary phase PGCGROWTHCONDITIONS
SRR5266619	GSE94984	GSM2493797	GPL23073	PMID_28245801	EHEC in LB Experiment 2 [RNA-Seq]	Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP	GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933)	organism	Escherichia coli O157:H7 str. EDL933 PGCGROWTHCONDITIONS
SRR5266619	GSE94984	GSM2493797	GPL23073	PMID_28245801	EHEC in LB Experiment 2 [RNA-Seq]	Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP	GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933)	source_name	biomass collected in the transition between exponential to stationary phase PGCGROWTHCONDITIONS
SRR5266619	GSE94984	GSM2493797	GPL23073	PMID_28245801	EHEC in LB Experiment 2 [RNA-Seq]	Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP	GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933)	contact_name	Klaus,,Neuhaus PGCGROWTHCONDITIONS