GSE94984-GSM2493797-GPL23073-PMID_28245801.tsv
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SRR5266619 GSE94984 GSM2493797 GPL23073 PMID_28245801 EHEC in LB Experiment 2 [RNA-Seq] Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933) data_processing Illumina fastq files were submitted to the galaxy pipeline => grooming => mapping to NC_002655 using bowtie2 (default but seed 19 nt & zero mismatches within seed) PGCGROWTHCONDITIONS
SRR5266619 GSE94984 GSM2493797 GPL23073 PMID_28245801 EHEC in LB Experiment 2 [RNA-Seq] Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933) data_processing Genome_build: NC_002655 PGCGROWTHCONDITIONS
SRR5266619 GSE94984 GSM2493797 GPL23073 PMID_28245801 EHEC in LB Experiment 2 [RNA-Seq] Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933) data_processing Supplementary_files_format_and_content: Reads, RPKM, and RCV mapped to NC_002655 (RCV = Ribosomal Coverage Value = RPKM-footprint over RPKM-transcription) PGCGROWTHCONDITIONS
SRR5266619 GSE94984 GSM2493797 GPL23073 PMID_28245801 EHEC in LB Experiment 2 [RNA-Seq] Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933) extract_protocol Trizol for RNAseq, gradient centrifugation => RNase digestions => Trizol => gel extraction for RIBOseq PGCGROWTHCONDITIONS
SRR5266619 GSE94984 GSM2493797 GPL23073 PMID_28245801 EHEC in LB Experiment 2 [RNA-Seq] Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933) extract_protocol Truseq Small RNA PGCGROWTHCONDITIONS
SRR5266619 GSE94984 GSM2493797 GPL23073 PMID_28245801 EHEC in LB Experiment 2 [RNA-Seq] Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933) extract_protocol ligation, rt-PCR, PCR PGCGROWTHCONDITIONS
SRR5266619 GSE94984 GSM2493797 GPL23073 PMID_28245801 EHEC in LB Experiment 2 [RNA-Seq] Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933) growth_protocol LB medium, 180 rpm shaking, at 37°C, between exponential and stationary phase PGCGROWTHCONDITIONS
SRR5266619 GSE94984 GSM2493797 GPL23073 PMID_28245801 EHEC in LB Experiment 2 [RNA-Seq] Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933) organism Escherichia coli O157:H7 str. EDL933 PGCGROWTHCONDITIONS
SRR5266619 GSE94984 GSM2493797 GPL23073 PMID_28245801 EHEC in LB Experiment 2 [RNA-Seq] Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933) source_name biomass collected in the transition between exponential to stationary phase PGCGROWTHCONDITIONS
SRR5266619 GSE94984 GSM2493797 GPL23073 PMID_28245801 EHEC in LB Experiment 2 [RNA-Seq] Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933) library_strategy RNA-Seq PGCGROWTHCONDITIONS
SRR5266619 GSE94984 GSM2493797 GPL23073 PMID_28245801 EHEC in LB Experiment 2 [RNA-Seq] Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933) characteristics growth media: LB medium PGCGROWTHCONDITIONS
SRR5266619 GSE94984 GSM2493797 GPL23073 PMID_28245801 EHEC in LB Experiment 2 [RNA-Seq] Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933) growth_protocol LB medium, 180 rpm shaking, at 37°C, between exponential and stationary phase PGCGROWTHCONDITIONS
SRR5266619 GSE94984 GSM2493797 GPL23073 PMID_28245801 EHEC in LB Experiment 2 [RNA-Seq] Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933) organism Escherichia coli O157:H7 str. EDL933 PGCGROWTHCONDITIONS
SRR5266619 GSE94984 GSM2493797 GPL23073 PMID_28245801 EHEC in LB Experiment 2 [RNA-Seq] Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933) source_name biomass collected in the transition between exponential to stationary phase PGCGROWTHCONDITIONS
SRR5266619 GSE94984 GSM2493797 GPL23073 PMID_28245801 EHEC in LB Experiment 2 [RNA-Seq] Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933) contact_name Klaus,,Neuhaus PGCGROWTHCONDITIONS