unique_content_labels.tsv
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Agit Air Anti Gtype Gversion Med Name OD Orgn Phase Strain Substrain Supp Technique Temp Vess
gyratory water bath shaking at 240 rpm aerobically (25% O2, 70% N2 and 5% CO2) 9E10 Myc tag fur-8myc NC_000913 LB Wild type control (10 min) OD600 of about 0.3 Escherichia coli mid-log phase K-12 MG1655star 100ug/L adenine RNA-Seq 30 °C (see control sample) flask
anerobically (95% N2, 5% CO2) Affinity Purified FNR antibody dFNR NC_000913 M9 minimal complete media NsrR_Flagtag_rep3 O.D.600nm 0.3 Escherichia coli str. K-12 substr. MG1655star mid-log phase K-12 MG1655 150 mg/mL ChIP-exo 37 °C
Aerobic SeqA ∆fnr U00096.2 glucose (2 g/L) minimal M9 medium NsrR_Flagtag_rep2 OD600 of 0.35 E. coli 16 µM IPTG and 300 µL Cm20 30 degrees C
aerobically Pre-cleared IHF polyclonal antibody dFNR (PK4854) M9 minimal media NsrR_Flagtag_rep1 OD600 of 0.3 Escherichia coli Fe and rifampicin 30 °C
anaerobic RNA polymerase subunit β Fur-8-myc L broth EC18n178 RpoH 2.5 min Time course 1 OD600 of about 0.15 Escherichia coli str. K-12 substr. MG1655 2g/L glutamine 37°C
anaerobically (95% N2 and 5% CO2) Pre-cleared FNR antibody WT MOPS Ecoli_wild-type_rep2_anaerobic 4 µM IPTG 43 °C
anaerobically RNA Polymerase ß monoclonal antibody Wild-Type glucose (2 g/L) minimal W2 medium HNS - Aerobic A 16 µM IPTG 43 °C (see heat sample)
Anaerobic Pre-cleared H-NS polyclonal antibody TrpR-8myc M9 minimal media HNS - Aerobic B 8 µM IPTG and 300 µL Cm20 37°C
σ32 PhtpG::lacZ delta lacX74/ptrc99A (control vector) M9 minimal medium ∆fnr - Anaeroibc 1g/L arginine
wildtype Ecoli_dFNR_rep1_anaerobic Fe
PK4854 Ptac::fnr - A - 8 µM IPTG nitrate
Flag-tag SeqA old deltaSeqA DPD and rifampicin
PK8263 SeqA old rep1 glucose
PhtpG::lacZ delta lacX74/pTG2 (vector carrying inducible Ptrc::rpoH) SeqA old rep2 20 mg/L tryptophan
PurR-8myc σ32 ChIP DNA, heat 0.1mM of FeCl2
ΔseqA PurR_glucose_2 4 µM IPTG and 300 µL Cm20
Fur-8-myc PurR_glucose_1 Adenine
Δfur SeqA new rep2 0.2% glucose
Lrp-8myc SeqA new rep1 rifampicin
NsrR EC18n185 RpoH 10 min Time course 3 8 µM IPTG
∆hns/∆stpA σ32 43°C rep2 0.2mM of DPD
ArgR-8myc σ32 43°C rep1 DPD
EC18n169 RpoH 5 min Time course 2 iron
HNS - Anaerobic A 10 mM leucine
HNS - Anaerobic B
Wild type control (0 min)
Ptac::fnr - B - 4 µM IPTG
RpoH induced (2.5 min)
IHF - Anaerobic B
IHF - Anaerobic A
RNAP old rep2
RpoH induced (0 min)
RNAP old rep1
EC18n122 RpoH 10 min Time course 4
Crosslink
RpoH induced (20 min)
Wild type control (20 min)
EC18n182 RpoH 0 min Time course 3
Lrp_Leu_1
Lrp_Leu_3
Lrp_Leu_2
EC18n171 RpoH 20 min Time course 2
Ptac::fnr - A - 16 µM IPTG
σ32 30°C short RNase
EC18n181 RpoH 20 min Time course 1
PurR_Adenine_2
PurR_Adenine_1
EC18n168 RpoH 2.5 min Time course 2
Lrp_NH4Cl_2
Lrp_NH4Cl_3
Lrp_NH4Cl_1
σ32 30°C rep1
σ32 30°C rep2
EC18n184 RpoH 5 min Time course 3
FNR - Anaerobic - A
FNR - Anaerobic - C
FNR - Anaerobic - B
Ptac::fnr - A - 4 µM IPTG
Ecoli_wild-type_rep1_anaerobic
EC18n167 RpoH 0 min Time course 2
FNR - ∆hns∆stpA A
FNR - ∆hns∆stpA B
Ptac::fnr - B - 8 µM IPTG
ArgR_Arginine_2
TrpR_glucose
ArgR_Arginine_1
Escherichia coli str. K-12 substr. MG1655
ArgR_NH4Cl_1
ArgR_NH4Cl_2
ß - Anaerobic - A
ß - Anaerobic - B
σ32 ChIP DNA, control
SeqA new deltaSeqA
EC18n177 RpoH 0 min Time course 1
TrpR_Trp
Ptac::fnr - B - 16 µM IPTG
ß - Aerobic - A
ß - Aerobic - B
σ32 43°C short RNase
RpoH induced (5 min)
FNR - Anaerobic - Affinity Purified - B
EC18n186 RpoH 20 min Time course 3
FNR - Anaerobic - Affinity Purified - A
Ptac::fnr - C - 16 µM IPTG
EC18n170 RpoH 10 min Time course 2
RpoH induced (10 min)
EC18n183 RpoH 2.5 min Time course 3
EC18n180 RpoH 10 min Time course 1
EC18n179 RpoH 5 min Time course 1
Wild type control (5 min)
Wild type control (2.5 min)
Ecoli_dFNR_rep2_anaerobic