full_map_srr_htregulondb.tsv
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BANGLINE CASE_MATCH ENTITY_NAME FULL_TEXT GPL GPL_NAME GSE GSE_NAME GSM GSM_NAME PMID REPO_FILE SET SORT SOURCE SOURCE_TEXT_CTRL SRR TERM_ID TERM_NAME TERM_TYPE MAP
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770989 Ribosome profiling at 37°C in WT cells_1 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001737 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770991 Ribosome profiling 30 min after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001739 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770994 Ribosome profiling 4 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001742 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770995 Ribosome profiling 6 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001743 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770996 Ribosome profiling 5 min after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001744 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770997 Ribosome profiling 10 min after shift to 10°C in WT cells_2 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001745 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770998 Ribosome profiling 15 min after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001746 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770999 Ribosome profiling 8 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001747SRR6001748 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771000 Ribosome profiling 8 hr after shift to 10°C in ∆cspABEG cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001749SRR6001750 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771001 Ribosome profiling at 37°C in WT cells_2 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001751 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771002 Ribosome profiling at 37°C in ∆cspABCEG cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001752 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771008 mRNA-seq 4 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001758 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771010 mRNA-seq 8 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001760SRR6001761 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771011 mRNA-seq 8 hr after shift to 10°C in ∆cspABEG cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001762SRR6001763 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771014 DMS-seq 30 min after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001766 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771015 DMS-seq 6 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001769 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771016 DMS-seq 8 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001772 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771017 DMS-seq 8 hr after shift to 10°C in ∆cspABEG cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001773 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771018 DMS-seq 8 hr after shift to 10°C in ∆cspBG cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001774 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771019 Total RNA-seq 20 min after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001775 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771020 Total RNA-seq 4 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001776 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771021 Total RNA-seq 8 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001777 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771022 Total RNA-seq 20 min after shift to 10°C in ∆rnr cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001778 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771023 Total RNA-seq 4 hr after shift to 10°C in ∆rnr cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001779 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771024 Total RNA-seq 8 hr after shift to 10°C in ∆rnr cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001780 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771025 Total RNA-seq before rifampicin treatment -- 2 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001781 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771026 Total RNA-seq after rifampicin treatment -- 4 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001782 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771027 Total RNA-seq before rifampicin treatment -- 2 hr after shift to 10°C in ∆rnr cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001783 MCO000003160 MOPS medium Medium True
growth_protocol MOPS rich medium All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771028 Total RNA-seq after rifampicin treatment -- 4 hr after shift to 10°C in ∆rnr cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001784 MCO000003160 MOPS medium Medium True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770989 Ribosome profiling at 37°C in WT cells_1 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001737 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770991 Ribosome profiling 30 min after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001739 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770994 Ribosome profiling 4 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001742 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770995 Ribosome profiling 6 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001743 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770996 Ribosome profiling 5 min after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001744 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770997 Ribosome profiling 10 min after shift to 10°C in WT cells_2 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001745 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770998 Ribosome profiling 15 min after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001746 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770999 Ribosome profiling 8 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001747SRR6001748 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771000 Ribosome profiling 8 hr after shift to 10°C in ∆cspABEG cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001749SRR6001750 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771001 Ribosome profiling at 37°C in WT cells_2 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001751 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771002 Ribosome profiling at 37°C in ∆cspABCEG cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001752 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771008 mRNA-seq 4 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001758 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771010 mRNA-seq 8 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001760SRR6001761 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771011 mRNA-seq 8 hr after shift to 10°C in ∆cspABEG cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001762SRR6001763 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771014 DMS-seq 30 min after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001766 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771015 DMS-seq 6 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001769 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771016 DMS-seq 8 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001772 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771017 DMS-seq 8 hr after shift to 10°C in ∆cspABEG cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001773 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771018 DMS-seq 8 hr after shift to 10°C in ∆cspBG cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001774 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771019 Total RNA-seq 20 min after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001775 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771020 Total RNA-seq 4 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001776 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771021 Total RNA-seq 8 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001777 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771022 Total RNA-seq 20 min after shift to 10°C in ∆rnr cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001778 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771023 Total RNA-seq 4 hr after shift to 10°C in ∆rnr cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001779 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771024 Total RNA-seq 8 hr after shift to 10°C in ∆rnr cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001780 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771025 Total RNA-seq before rifampicin treatment -- 2 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001781 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771026 Total RNA-seq after rifampicin treatment -- 4 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001782 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771027 Total RNA-seq before rifampicin treatment -- 2 hr after shift to 10°C in ∆rnr cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001783 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771028 Total RNA-seq after rifampicin treatment -- 4 hr after shift to 10°C in ∆rnr cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001784 MCO000002770 0.2 % glucose Medium supplement True
characteristics glucose 0.2% media : <Med> M9 minimal media </Med> w / <Supp> 0.2 % glucose </Supp> and 10mM glutamate GPL17024-GPL17439-GPL18995-GPL25769 GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655). GPL18995: Illumina MiSeq (Escherichia coli BW25113). GPL25769: Illumina Genome Analyzer IIx (Escherichia coli BW25113) GSE122211 Expression profiling of E. coli K-12 MG1655 GSM3461164 MG_glu_1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122211/GSE122211.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 0.2% glucose and 10mM glutamate PGCGROWTHCONDITIONS SRR8164484 MCO000002770 0.2 % glucose Medium supplement True
characteristics glucose 0.2% media : <Med> M9 minimal media </Med> w / <Supp> 0.2 % glucose </Supp> and 10mM glutamate GPL17024-GPL17439-GPL18995-GPL25769 GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655). GPL18995: Illumina MiSeq (Escherichia coli BW25113). GPL25769: Illumina Genome Analyzer IIx (Escherichia coli BW25113) GSE122211 Expression profiling of E. coli K-12 MG1655 GSM3461165 MG_glu_2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122211/GSE122211.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 0.2% glucose and 10mM glutamate PGCGROWTHCONDITIONS SRR8164485 MCO000002770 0.2 % glucose Medium supplement True
characteristics glucose 0.2% media : <Med> M9 minimal media </Med> w / <Supp> 0.2 % glucose </Supp> GPL17024-GPL17439-GPL18995-GPL25769 GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655). GPL18995: Illumina MiSeq (Escherichia coli BW25113). GPL25769: Illumina Genome Analyzer IIx (Escherichia coli BW25113) GSE122211 Expression profiling of E. coli K-12 MG1655 GSM3461166 MG_glc 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122211/GSE122211.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 0.2% glucose PGCGROWTHCONDITIONS SRR8164486 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K12 MG1655 was grown to <Phase> mid-log phase </Phase> ( <OD> O.D. 600nm 0.5 </OD> ) or to stationary phase ( <OD> O.D. 600nm 1.5 </OD> ) <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> or <Med> W2 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> and 0.2 % glutamine . For heatshock conditions , cells were grown to mid-log phase and incubated at 42oC for 10 min . GPL17137 GPL17137: Illumina Genome Analyzer (Escherichia coli str. K-12 substr. MG1655) GSE46737 Genome-scale reconstruction of the sigma factor network in E. coli GSM1137316 E. coli stationary 1 24461193 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE46737/GSE46737.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 MG1655 was grown to mid-log phase (O.D.600nm 0.5) or to stationary phase (O.D.600nm 1.5) aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose or W2 minimal media supplemented with 0.2% glucose and 0.2% glutamine. For heatshock conditions, cells were grown to mid-log phase and incubated at 42oC for 10 min. PGCGROWTHCONDITIONS SRR847728 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K12 MG1655 was grown to <Phase> mid-log phase </Phase> ( <OD> O.D. 600nm 0.5 </OD> ) or to stationary phase ( <OD> O.D. 600nm 1.5 </OD> ) <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> or <Med> W2 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> and 0.2 % glutamine . For heatshock conditions , cells were grown to mid-log phase and incubated at 42oC for 10 min . GPL17137 GPL17137: Illumina Genome Analyzer (Escherichia coli str. K-12 substr. MG1655) GSE46737 Genome-scale reconstruction of the sigma factor network in E. coli GSM1137318 E. coli glutamine 1 24461193 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE46737/GSE46737.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 MG1655 was grown to mid-log phase (O.D.600nm 0.5) or to stationary phase (O.D.600nm 1.5) aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose or W2 minimal media supplemented with 0.2% glucose and 0.2% glutamine. For heatshock conditions, cells were grown to mid-log phase and incubated at 42oC for 10 min. PGCGROWTHCONDITIONS SRR847730 MCO000002770 0.2 % glucose Medium supplement True
source_name glucose 0.2% Cells at mid-log phase ( OD600nm 0.5 ) in W2 media supplemented with <Supp> 0.2 % glucose </Supp> and <Supp> 0.2 % glutamine </Supp> GPL17137 GPL17137: Illumina Genome Analyzer (Escherichia coli str. K-12 substr. MG1655) GSE46737 Genome-scale reconstruction of the sigma factor network in E. coli GSM1137318 E. coli glutamine 1 24461193 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE46737/GSE46737.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells at mid-log phase (OD600nm 0.5) in W2 media supplemented with 0.2% glucose and 0.2% glutamine PGCGROWTHCONDITIONS SRR847730 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K12 MG1655 was grown to <Phase> mid-log phase </Phase> ( <OD> O.D. 600nm 0.5 </OD> ) or to stationary phase ( <OD> O.D. 600nm 1.5 </OD> ) <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> or <Med> W2 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> and 0.2 % glutamine . For heatshock conditions , cells were grown to mid-log phase and incubated at 42oC for 10 min . GPL17137 GPL17137: Illumina Genome Analyzer (Escherichia coli str. K-12 substr. MG1655) GSE46737 Genome-scale reconstruction of the sigma factor network in E. coli GSM1137319 E. coli glutamine 2 24461193 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE46737/GSE46737.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 MG1655 was grown to mid-log phase (O.D.600nm 0.5) or to stationary phase (O.D.600nm 1.5) aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose or W2 minimal media supplemented with 0.2% glucose and 0.2% glutamine. For heatshock conditions, cells were grown to mid-log phase and incubated at 42oC for 10 min. PGCGROWTHCONDITIONS SRR847731 MCO000002770 0.2 % glucose Medium supplement True
source_name glucose 0.2% Cells at mid-log phase ( OD600nm 0.5 ) in W2 media supplemented with <Supp> 0.2 % glucose </Supp> and <Supp> 0.2 % glutamine </Supp> GPL17137 GPL17137: Illumina Genome Analyzer (Escherichia coli str. K-12 substr. MG1655) GSE46737 Genome-scale reconstruction of the sigma factor network in E. coli GSM1137319 E. coli glutamine 2 24461193 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE46737/GSE46737.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells at mid-log phase (OD600nm 0.5) in W2 media supplemented with 0.2% glucose and 0.2% glutamine PGCGROWTHCONDITIONS SRR847731 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K12 MG1655 was grown to <Phase> mid-log phase </Phase> ( <OD> O.D. 600nm 0.5 </OD> ) or to stationary phase ( <OD> O.D. 600nm 1.5 </OD> ) <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> or <Med> W2 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> and 0.2 % glutamine . For heatshock conditions , cells were grown to mid-log phase and incubated at 42oC for 10 min . GPL17137 GPL17137: Illumina Genome Analyzer (Escherichia coli str. K-12 substr. MG1655) GSE46737 Genome-scale reconstruction of the sigma factor network in E. coli GSM1137320 E. coli heatshock 1 24461193 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE46737/GSE46737.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 MG1655 was grown to mid-log phase (O.D.600nm 0.5) or to stationary phase (O.D.600nm 1.5) aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose or W2 minimal media supplemented with 0.2% glucose and 0.2% glutamine. For heatshock conditions, cells were grown to mid-log phase and incubated at 42oC for 10 min. PGCGROWTHCONDITIONS SRR847732 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K12 MG1655 was grown to <Phase> mid-log phase </Phase> ( <OD> O.D. 600nm 0.5 </OD> ) or to stationary phase ( <OD> O.D. 600nm 1.5 </OD> ) <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> or <Med> W2 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> and 0.2 % glutamine . For heatshock conditions , cells were grown to mid-log phase and incubated at 42oC for 10 min . GPL17137 GPL17137: Illumina Genome Analyzer (Escherichia coli str. K-12 substr. MG1655) GSE46737 Genome-scale reconstruction of the sigma factor network in E. coli GSM1137321 E. coli heatshock 2 24461193 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE46737/GSE46737.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 MG1655 was grown to mid-log phase (O.D.600nm 0.5) or to stationary phase (O.D.600nm 1.5) aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose or W2 minimal media supplemented with 0.2% glucose and 0.2% glutamine. For heatshock conditions, cells were grown to mid-log phase and incubated at 42oC for 10 min. PGCGROWTHCONDITIONS SRR847733 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All cultures were based on MOPS media with <Supp> 0.2 % glucose </Supp> ( Teknova ) , with either full supplement ( Neidhardt et al. , 1974 ) . An overnight liquid culture was diluted 400-fold into 200 ml <Med> fresh media </Med> . The culture was kept in a 2.8-liter flask at 37C with aeration ( 180 rpm ) until OD600 reached 0.3 . GPL14548-GPL18133 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE53767 Absolute quantification of protein production reveals principles underlying protein synthesis rates GSM1300282 mRNA-seq in rich defined media 24766808 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE53767/GSE53767.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All cultures were based on MOPS media with 0.2% glucose (Teknova), with either full supplement (Neidhardt et al., 1974). An overnight liquid culture was diluted 400-fold into 200 ml fresh media. The culture was kept in a 2.8-liter flask at 37C with aeration (180 rpm) until OD600 reached 0.3. PGCGROWTHCONDITIONS SRR1067773SRR1067774 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT and Δfur were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . For iron treated cells , 0.1 mM of FeCl2 were treated from the beginning of culture , and for DPD treated cells , <Supp> 0.2 mM of DPD </Supp> were added at early-log phase and continued to culture for additional <Supp> 2h </Supp> . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326347 WT with Fe 1 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. PGCGROWTHCONDITIONS SRR1168133 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT and Δfur were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . For iron treated cells , 0.1 mM of FeCl2 were treated from the beginning of culture , and for DPD treated cells , <Supp> 0.2 mM of DPD </Supp> were added at early-log phase and continued to culture for additional <Supp> 2h </Supp> . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326348 WT with Fe 2 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. PGCGROWTHCONDITIONS SRR1168134 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT and Δfur were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . For iron treated cells , 0.1 mM of FeCl2 were treated from the beginning of culture , and for DPD treated cells , <Supp> 0.2 mM of DPD </Supp> were added at early-log phase and continued to culture for additional <Supp> 2h </Supp> . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326349 WT with DPD 1 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. PGCGROWTHCONDITIONS SRR1168135 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT and Δfur were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . For iron treated cells , 0.1 mM of FeCl2 were treated from the beginning of culture , and for DPD treated cells , <Supp> 0.2 mM of DPD </Supp> were added at early-log phase and continued to culture for additional <Supp> 2h </Supp> . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326350 WT with DPD 2 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. PGCGROWTHCONDITIONS SRR1168136 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT and Δfur were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . For iron treated cells , 0.1 mM of FeCl2 were treated from the beginning of culture , and for DPD treated cells , <Supp> 0.2 mM of DPD </Supp> were added at early-log phase and continued to culture for additional <Supp> 2h </Supp> . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326351 Δfur with Fe 1 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. PGCGROWTHCONDITIONS SRR1168137 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT and Δfur were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . For iron treated cells , 0.1 mM of FeCl2 were treated from the beginning of culture , and for DPD treated cells , <Supp> 0.2 mM of DPD </Supp> were added at early-log phase and continued to culture for additional <Supp> 2h </Supp> . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326352 Δfur with Fe 2 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. PGCGROWTHCONDITIONS SRR1168138 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT and Δfur were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . For iron treated cells , 0.1 mM of FeCl2 were treated from the beginning of culture , and for DPD treated cells , <Supp> 0.2 mM of DPD </Supp> were added at early-log phase and continued to culture for additional <Supp> 2h </Supp> . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326353 Δfur with DPD 1 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. PGCGROWTHCONDITIONS SRR1168139 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT and Δfur were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . For iron treated cells , 0.1 mM of FeCl2 were treated from the beginning of culture , and for DPD treated cells , <Supp> 0.2 mM of DPD </Supp> were added at early-log phase and continued to culture for additional <Supp> 2h </Supp> . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326354 Δfur with DPD 2 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. PGCGROWTHCONDITIONS SRR1168140 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% <Gtype> Wild type </Gtype> E. coli K-12 ( strain BW38038 ) and BW39452 ( ΔrpoS ) cultures were grown on <Med> MOPS glucose minimal medium </Med> with <Supp> 0.2 % glucose </Supp> as sole carbon source at <Temp> 37 °C </Temp> , pH was initially 7.4 , and the agitation speed was 500 rpm . Culture samples were harvested during logarithmic growth and following entry into stationary phase for the WT and rpoS mutant . OD600 measurements were made on a Beckman Coulter DU 800 spectrophotometer . Samples were harvested directly into ice-cold RNAlater at a 1:1 dilution to protect RNA from degradation and cells then were pelleted by centrifugation at 8000rpm for 10 minutes . Cell pellets were stored at -80 °C in an equal volume of RNAlater prior to RNA extraction . GPL18814 GPL18814: Illumina HiSeq 2000 (Escherichia coli BW38028) GSE58556 RNA sequencing based analysis of the bacterial transcriptome GSM1413874 WT_glucose_log 25483350 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE58556/GSE58556.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Wild type E. coli K-12 (strain BW38038) and BW39452(ΔrpoS) cultures were grown on MOPS glucose minimal medium with 0.2% glucose as sole carbon source at 37°C, pH was initially 7.4, and the agitation speed was 500 rpm. Culture samples were harvested during logarithmic growth and following entry into stationary phase for the WT and rpoS mutant. OD600 measurements were made on a Beckman Coulter DU 800 spectrophotometer. Samples were harvested directly into ice-cold RNAlater at a 1:1 dilution to protect RNA from degradation and cells then were pelleted by centrifugation at 8000rpm for 10 minutes. Cell pellets were stored at -80°C in an equal volume of RNAlater prior to RNA extraction. PGCGROWTHCONDITIONS SRR1411272 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602347 WT glucose 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787590 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602348 WT glucose 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787591 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602349 WT fructose 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787592 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602350 WT fructose 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787593 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602351 WT acetate 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787594 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602352 WT acetate 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787595 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602353 Δcra glucose 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787596 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602354 Δcra glucose 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787597 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602355 Δcra fructose 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787598 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602356 Δcra fructose 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787599 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602357 Δcra acetate 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787600 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602358 Δcra acetate 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787601 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT , ΔoxyR , ΔsoxR , and ΔsoxS were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603386 WT PQ 1 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, ΔoxyR, ΔsoxR, and ΔsoxS were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation. PGCGROWTHCONDITIONS SRR1796598 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT , ΔoxyR , ΔsoxR , and ΔsoxS were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603387 WT PQ 2 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, ΔoxyR, ΔsoxR, and ΔsoxS were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation. PGCGROWTHCONDITIONS SRR1796599 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT , ΔoxyR , ΔsoxR , and ΔsoxS were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603388 ΔoxyR PQ 1 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, ΔoxyR, ΔsoxR, and ΔsoxS were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation. PGCGROWTHCONDITIONS SRR1796600 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT , ΔoxyR , ΔsoxR , and ΔsoxS were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603389 ΔoxyR PQ 2 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, ΔoxyR, ΔsoxR, and ΔsoxS were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation. PGCGROWTHCONDITIONS SRR1796601 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT , ΔoxyR , ΔsoxR , and ΔsoxS were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603391 ΔsoxR PQ 2 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, ΔoxyR, ΔsoxR, and ΔsoxS were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation. PGCGROWTHCONDITIONS SRR1796603 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT , ΔoxyR , ΔsoxR , and ΔsoxS were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603392 ΔsoxS PQ 1 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, ΔoxyR, ΔsoxR, and ΔsoxS were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation. PGCGROWTHCONDITIONS SRR1796604 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT , ΔoxyR , ΔsoxR , and ΔsoxS were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603393 ΔsoxS PQ 2 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, ΔoxyR, ΔsoxR, and ΔsoxS were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation. PGCGROWTHCONDITIONS SRR1796605 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623160 WT pH5.5 1 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824557 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623161 WT pH5.5 2 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824558 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623162 ΔgadE pH5.5 1 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824559 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623163 ΔgadE pH5.5 2 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824560 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623164 ΔgadW pH5.5 1 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824561 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623165 ΔgadW pH5.5 2 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824562 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623166 ΔgadX pH5.5 1 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824563 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623167 ΔgadX pH5.5 2 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824564 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All cultures were based on MOPS media with <Supp> 0.2 % glucose </Supp> ( Teknova ) , with full supplement ( Neidhardt et al. , 1974 ) minus methionine . An overnight liquid culture was diluted 400-fold into 200 ml <Med> fresh media </Med> . The culture was kept in a 1 liter flask at <Temp> 37 °C </Temp> with aeration until OD420 reached 0.4 . GPL14548-GPL21433 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE77617 Operon mRNAs are organized into ORF-centric structures that predict translation efficiency GSM2462936 mRNA-seq 37°C in WT with control plasmid 28139975 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE77617/GSE77617.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All cultures were based on MOPS media with 0.2% glucose (Teknova), with full supplement (Neidhardt et al., 1974) minus methionine. An overnight liquid culture was diluted 400-fold into 200 ml fresh media. The culture was kept in a 1 liter flask at 37°C with aeration until OD420 reached 0.4. PGCGROWTHCONDITIONS SRR5186139 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All cultures were based on MOPS media with <Supp> 0.2 % glucose </Supp> ( Teknova ) , with full supplement ( Neidhardt et al. , 1974 ) minus methionine . An overnight liquid culture was diluted 400-fold into 200 ml <Med> fresh media </Med> . The culture was kept in a 1 liter flask at <Temp> 37 °C </Temp> with aeration until OD420 reached 0.4 . GPL14548-GPL21433 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE77617 Operon mRNAs are organized into ORF-centric structures that predict translation efficiency GSM2462937 mRNA-seq 37°C in WT with plasmid expressing mini-ORF CUA 28139975 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE77617/GSE77617.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All cultures were based on MOPS media with 0.2% glucose (Teknova), with full supplement (Neidhardt et al., 1974) minus methionine. An overnight liquid culture was diluted 400-fold into 200 ml fresh media. The culture was kept in a 1 liter flask at 37°C with aeration until OD420 reached 0.4. PGCGROWTHCONDITIONS SRR5186140 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% All cultures were based on MOPS media with <Supp> 0.2 % glucose </Supp> ( Teknova ) , with full supplement ( Neidhardt et al. , 1974 ) minus methionine . An overnight liquid culture was diluted 400-fold into 200 ml <Med> fresh media </Med> . The culture was kept in a 1 liter flask at <Temp> 37 °C </Temp> with aeration until OD420 reached 0.4 . GPL14548-GPL21433 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE77617 Operon mRNAs are organized into ORF-centric structures that predict translation efficiency GSM2462938 mRNA-seq 37°C in WT with plasmid expressing mini-ORF CUG 28139975 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE77617/GSE77617.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All cultures were based on MOPS media with 0.2% glucose (Teknova), with full supplement (Neidhardt et al., 1974) minus methionine. An overnight liquid culture was diluted 400-fold into 200 ml fresh media. The culture was kept in a 1 liter flask at 37°C with aeration until OD420 reached 0.4. PGCGROWTHCONDITIONS SRR5186141 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT and ΔompR were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with <Supp> 0.3 M of NaCl </Supp> at mid-log pahse for <Supp> 30 min </Supp> with agitation . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE88980 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GSM2356687 WT NaCl 1 28526842 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88980/GSE88980.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and ΔompR were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 0.3 M of NaCl at mid-log pahse for 30 min with agitation. PGCGROWTHCONDITIONS SRR4435464 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT and ΔompR were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with <Supp> 0.3 M of NaCl </Supp> at mid-log pahse for <Supp> 30 min </Supp> with agitation . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE88980 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GSM2356688 WT NaCl 2 28526842 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88980/GSE88980.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and ΔompR were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 0.3 M of NaCl at mid-log pahse for 30 min with agitation. PGCGROWTHCONDITIONS SRR4435465 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT and ΔompR were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with <Supp> 0.3 M of NaCl </Supp> at mid-log pahse for <Supp> 30 min </Supp> with agitation . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE88980 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GSM2356689 ΔompR NaCl 1 28526842 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88980/GSE88980.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and ΔompR were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 0.3 M of NaCl at mid-log pahse for 30 min with agitation. PGCGROWTHCONDITIONS SRR4435466 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% E. coli K-12 MG1655 WT and ΔompR were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with <Supp> 0.3 M of NaCl </Supp> at mid-log pahse for <Supp> 30 min </Supp> with agitation . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE88980 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GSM2356690 ΔompR NaCl 2 28526842 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88980/GSE88980.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and ΔompR were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 0.3 M of NaCl at mid-log pahse for 30 min with agitation. PGCGROWTHCONDITIONS SRR4435467 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% Duplicate O/N cultures in LB with 15 mg/L chloramphenicol each of strains pCA24N,-gfp / DA4201 , Svi3-3 comp . ( recoded Svi3-3 ) / DA4201 and pORF1/DA4201 were diluted 1:100 into two , four and four cultures of 20 mL M9 + <Supp> 0.2 % glucose </Supp> with 5 μg/mL chloramphenicol . The pCA24N,-gfp / DA4201 cultures and two cultures each of Svi3-3 comp . ( recoded Svi3-3 ) / DA4201 and pORF1/DA4201 were supplemented by 50 μM IPTG . The cells were grown to OD 0.15-0.2 and cells were withdrawn for RNA extraction and whole cell proteomics . GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433290 ORF1_1_IPTG 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Duplicate O/N cultures in LB with 15 mg/L chloramphenicol each of strains pCA24N,-gfp/DA4201, Svi3-3 comp. (recoded Svi3-3)/DA4201 and pORF1/DA4201 were diluted 1:100 into two, four and four cultures of 20 mL M9 + 0.2% glucose with 5 μg/mL chloramphenicol. The pCA24N,-gfp/DA4201 cultures and two cultures each of Svi3-3 comp. (recoded Svi3-3)/DA4201 and pORF1/DA4201 were supplemented by 50 μM IPTG. The cells were grown to OD 0.15-0.2 and cells were withdrawn for RNA extraction and whole cell proteomics. PGCGROWTHCONDITIONS SRR5121109 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% Duplicate O/N cultures in LB with 15 mg/L chloramphenicol each of strains pCA24N,-gfp / DA4201 , Svi3-3 comp . ( recoded Svi3-3 ) / DA4201 and pORF1/DA4201 were diluted 1:100 into two , four and four cultures of 20 mL M9 + <Supp> 0.2 % glucose </Supp> with 5 μg/mL chloramphenicol . The pCA24N,-gfp / DA4201 cultures and two cultures each of Svi3-3 comp . ( recoded Svi3-3 ) / DA4201 and pORF1/DA4201 were supplemented by 50 μM IPTG . The cells were grown to OD 0.15-0.2 and cells were withdrawn for RNA extraction and whole cell proteomics . GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433291 ORF1_1 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Duplicate O/N cultures in LB with 15 mg/L chloramphenicol each of strains pCA24N,-gfp/DA4201, Svi3-3 comp. (recoded Svi3-3)/DA4201 and pORF1/DA4201 were diluted 1:100 into two, four and four cultures of 20 mL M9 + 0.2% glucose with 5 μg/mL chloramphenicol. The pCA24N,-gfp/DA4201 cultures and two cultures each of Svi3-3 comp. (recoded Svi3-3)/DA4201 and pORF1/DA4201 were supplemented by 50 μM IPTG. The cells were grown to OD 0.15-0.2 and cells were withdrawn for RNA extraction and whole cell proteomics. PGCGROWTHCONDITIONS SRR5121110 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% Duplicate O/N cultures in LB with 15 mg/L chloramphenicol each of strains pCA24N,-gfp / DA4201 , Svi3-3 comp . ( recoded Svi3-3 ) / DA4201 and pORF1/DA4201 were diluted 1:100 into two , four and four cultures of 20 mL M9 + <Supp> 0.2 % glucose </Supp> with 5 μg/mL chloramphenicol . The pCA24N,-gfp / DA4201 cultures and two cultures each of Svi3-3 comp . ( recoded Svi3-3 ) / DA4201 and pORF1/DA4201 were supplemented by 50 μM IPTG . The cells were grown to OD 0.15-0.2 and cells were withdrawn for RNA extraction and whole cell proteomics . GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433292 ORF1_2_IPTG 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Duplicate O/N cultures in LB with 15 mg/L chloramphenicol each of strains pCA24N,-gfp/DA4201, Svi3-3 comp. (recoded Svi3-3)/DA4201 and pORF1/DA4201 were diluted 1:100 into two, four and four cultures of 20 mL M9 + 0.2% glucose with 5 μg/mL chloramphenicol. The pCA24N,-gfp/DA4201 cultures and two cultures each of Svi3-3 comp. (recoded Svi3-3)/DA4201 and pORF1/DA4201 were supplemented by 50 μM IPTG. The cells were grown to OD 0.15-0.2 and cells were withdrawn for RNA extraction and whole cell proteomics. PGCGROWTHCONDITIONS SRR5121111 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% Duplicate O/N cultures in LB with 15 mg/L chloramphenicol each of strains pCA24N,-gfp / DA4201 , Svi3-3 comp . ( recoded Svi3-3 ) / DA4201 and pORF1/DA4201 were diluted 1:100 into two , four and four cultures of 20 mL M9 + <Supp> 0.2 % glucose </Supp> with 5 μg/mL chloramphenicol . The pCA24N,-gfp / DA4201 cultures and two cultures each of Svi3-3 comp . ( recoded Svi3-3 ) / DA4201 and pORF1/DA4201 were supplemented by 50 μM IPTG . The cells were grown to OD 0.15-0.2 and cells were withdrawn for RNA extraction and whole cell proteomics . GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433293 ORF1_2 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Duplicate O/N cultures in LB with 15 mg/L chloramphenicol each of strains pCA24N,-gfp/DA4201, Svi3-3 comp. (recoded Svi3-3)/DA4201 and pORF1/DA4201 were diluted 1:100 into two, four and four cultures of 20 mL M9 + 0.2% glucose with 5 μg/mL chloramphenicol. The pCA24N,-gfp/DA4201 cultures and two cultures each of Svi3-3 comp. (recoded Svi3-3)/DA4201 and pORF1/DA4201 were supplemented by 50 μM IPTG. The cells were grown to OD 0.15-0.2 and cells were withdrawn for RNA extraction and whole cell proteomics. PGCGROWTHCONDITIONS SRR5121112 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% Duplicate O/N cultures in LB with 15 mg/L chloramphenicol each of strains pCA24N,-gfp / DA4201 , Svi3-3 comp . ( recoded Svi3-3 ) / DA4201 and pORF1/DA4201 were diluted 1:100 into two , four and four cultures of 20 mL M9 + <Supp> 0.2 % glucose </Supp> with 5 μg/mL chloramphenicol . The pCA24N,-gfp / DA4201 cultures and two cultures each of Svi3-3 comp . ( recoded Svi3-3 ) / DA4201 and pORF1/DA4201 were supplemented by 50 μM IPTG . The cells were grown to OD 0.15-0.2 and cells were withdrawn for RNA extraction and whole cell proteomics . GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433294 Svi3_3_1_IPTG 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Duplicate O/N cultures in LB with 15 mg/L chloramphenicol each of strains pCA24N,-gfp/DA4201, Svi3-3 comp. (recoded Svi3-3)/DA4201 and pORF1/DA4201 were diluted 1:100 into two, four and four cultures of 20 mL M9 + 0.2% glucose with 5 μg/mL chloramphenicol. The pCA24N,-gfp/DA4201 cultures and two cultures each of Svi3-3 comp. (recoded Svi3-3)/DA4201 and pORF1/DA4201 were supplemented by 50 μM IPTG. The cells were grown to OD 0.15-0.2 and cells were withdrawn for RNA extraction and whole cell proteomics. PGCGROWTHCONDITIONS SRR5121113 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% Duplicate O/N cultures in LB with 15 mg/L chloramphenicol each of strains pCA24N,-gfp / DA4201 , Svi3-3 comp . ( recoded Svi3-3 ) / DA4201 and pORF1/DA4201 were diluted 1:100 into two , four and four cultures of 20 mL M9 + <Supp> 0.2 % glucose </Supp> with 5 μg/mL chloramphenicol . The pCA24N,-gfp / DA4201 cultures and two cultures each of Svi3-3 comp . ( recoded Svi3-3 ) / DA4201 and pORF1/DA4201 were supplemented by 50 μM IPTG . The cells were grown to OD 0.15-0.2 and cells were withdrawn for RNA extraction and whole cell proteomics . GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433295 Svi3_3_1 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Duplicate O/N cultures in LB with 15 mg/L chloramphenicol each of strains pCA24N,-gfp/DA4201, Svi3-3 comp. (recoded Svi3-3)/DA4201 and pORF1/DA4201 were diluted 1:100 into two, four and four cultures of 20 mL M9 + 0.2% glucose with 5 μg/mL chloramphenicol. The pCA24N,-gfp/DA4201 cultures and two cultures each of Svi3-3 comp. (recoded Svi3-3)/DA4201 and pORF1/DA4201 were supplemented by 50 μM IPTG. The cells were grown to OD 0.15-0.2 and cells were withdrawn for RNA extraction and whole cell proteomics. PGCGROWTHCONDITIONS SRR5121114 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% Duplicate O/N cultures in LB with 15 mg/L chloramphenicol each of strains pCA24N,-gfp / DA4201 , Svi3-3 comp . ( recoded Svi3-3 ) / DA4201 and pORF1/DA4201 were diluted 1:100 into two , four and four cultures of 20 mL M9 + <Supp> 0.2 % glucose </Supp> with 5 μg/mL chloramphenicol . The pCA24N,-gfp / DA4201 cultures and two cultures each of Svi3-3 comp . ( recoded Svi3-3 ) / DA4201 and pORF1/DA4201 were supplemented by 50 μM IPTG . The cells were grown to OD 0.15-0.2 and cells were withdrawn for RNA extraction and whole cell proteomics . GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433296 Svi3_3_2_IPTG 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Duplicate O/N cultures in LB with 15 mg/L chloramphenicol each of strains pCA24N,-gfp/DA4201, Svi3-3 comp. (recoded Svi3-3)/DA4201 and pORF1/DA4201 were diluted 1:100 into two, four and four cultures of 20 mL M9 + 0.2% glucose with 5 μg/mL chloramphenicol. The pCA24N,-gfp/DA4201 cultures and two cultures each of Svi3-3 comp. (recoded Svi3-3)/DA4201 and pORF1/DA4201 were supplemented by 50 μM IPTG. The cells were grown to OD 0.15-0.2 and cells were withdrawn for RNA extraction and whole cell proteomics. PGCGROWTHCONDITIONS SRR5121115 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% Duplicate O/N cultures in LB with 15 mg/L chloramphenicol each of strains pCA24N,-gfp / DA4201 , Svi3-3 comp . ( recoded Svi3-3 ) / DA4201 and pORF1/DA4201 were diluted 1:100 into two , four and four cultures of 20 mL M9 + <Supp> 0.2 % glucose </Supp> with 5 μg/mL chloramphenicol . The pCA24N,-gfp / DA4201 cultures and two cultures each of Svi3-3 comp . ( recoded Svi3-3 ) / DA4201 and pORF1/DA4201 were supplemented by 50 μM IPTG . The cells were grown to OD 0.15-0.2 and cells were withdrawn for RNA extraction and whole cell proteomics . GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433297 Svi3_3_2 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Duplicate O/N cultures in LB with 15 mg/L chloramphenicol each of strains pCA24N,-gfp/DA4201, Svi3-3 comp. (recoded Svi3-3)/DA4201 and pORF1/DA4201 were diluted 1:100 into two, four and four cultures of 20 mL M9 + 0.2% glucose with 5 μg/mL chloramphenicol. The pCA24N,-gfp/DA4201 cultures and two cultures each of Svi3-3 comp. (recoded Svi3-3)/DA4201 and pORF1/DA4201 were supplemented by 50 μM IPTG. The cells were grown to OD 0.15-0.2 and cells were withdrawn for RNA extraction and whole cell proteomics. PGCGROWTHCONDITIONS SRR5121116 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% Duplicate O/N cultures in LB with 15 mg/L chloramphenicol each of strains pCA24N,-gfp / DA4201 , Svi3-3 comp . ( recoded Svi3-3 ) / DA4201 and pORF1/DA4201 were diluted 1:100 into two , four and four cultures of 20 mL M9 + <Supp> 0.2 % glucose </Supp> with 5 μg/mL chloramphenicol . The pCA24N,-gfp / DA4201 cultures and two cultures each of Svi3-3 comp . ( recoded Svi3-3 ) / DA4201 and pORF1/DA4201 were supplemented by 50 μM IPTG . The cells were grown to OD 0.15-0.2 and cells were withdrawn for RNA extraction and whole cell proteomics . GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433298 WT1_IPTG 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Duplicate O/N cultures in LB with 15 mg/L chloramphenicol each of strains pCA24N,-gfp/DA4201, Svi3-3 comp. (recoded Svi3-3)/DA4201 and pORF1/DA4201 were diluted 1:100 into two, four and four cultures of 20 mL M9 + 0.2% glucose with 5 μg/mL chloramphenicol. The pCA24N,-gfp/DA4201 cultures and two cultures each of Svi3-3 comp. (recoded Svi3-3)/DA4201 and pORF1/DA4201 were supplemented by 50 μM IPTG. The cells were grown to OD 0.15-0.2 and cells were withdrawn for RNA extraction and whole cell proteomics. PGCGROWTHCONDITIONS SRR5121117 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol glucose 0.2% Duplicate O/N cultures in LB with 15 mg/L chloramphenicol each of strains pCA24N,-gfp / DA4201 , Svi3-3 comp . ( recoded Svi3-3 ) / DA4201 and pORF1/DA4201 were diluted 1:100 into two , four and four cultures of 20 mL M9 + <Supp> 0.2 % glucose </Supp> with 5 μg/mL chloramphenicol . The pCA24N,-gfp / DA4201 cultures and two cultures each of Svi3-3 comp . ( recoded Svi3-3 ) / DA4201 and pORF1/DA4201 were supplemented by 50 μM IPTG . The cells were grown to OD 0.15-0.2 and cells were withdrawn for RNA extraction and whole cell proteomics . GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433299 WT2_IPTG 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Duplicate O/N cultures in LB with 15 mg/L chloramphenicol each of strains pCA24N,-gfp/DA4201, Svi3-3 comp. (recoded Svi3-3)/DA4201 and pORF1/DA4201 were diluted 1:100 into two, four and four cultures of 20 mL M9 + 0.2% glucose with 5 μg/mL chloramphenicol. The pCA24N,-gfp/DA4201 cultures and two cultures each of Svi3-3 comp. (recoded Svi3-3)/DA4201 and pORF1/DA4201 were supplemented by 50 μM IPTG. The cells were grown to OD 0.15-0.2 and cells were withdrawn for RNA extraction and whole cell proteomics. PGCGROWTHCONDITIONS SRR5121118 MCO000002770 0.2 % glucose Medium supplement True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770989 Ribosome profiling at 37°C in WT cells_1 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001737 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770989 Ribosome profiling at 37°C in WT cells_1 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001737 MCO000003199 37 °C Temperature True
characteristics 35.7 C temperature : <Temp> 37 °C </Temp> GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770989 Ribosome profiling at 37°C in WT cells_1 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt temperature: 37°C PGCGROWTHCONDITIONS SRR6001737 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770991 Ribosome profiling 30 min after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001739 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770991 Ribosome profiling 30 min after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001739 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770994 Ribosome profiling 4 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001742 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770994 Ribosome profiling 4 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001742 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770995 Ribosome profiling 6 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001743 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770995 Ribosome profiling 6 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001743 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770996 Ribosome profiling 5 min after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001744 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770996 Ribosome profiling 5 min after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001744 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770997 Ribosome profiling 10 min after shift to 10°C in WT cells_2 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001745 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770997 Ribosome profiling 10 min after shift to 10°C in WT cells_2 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001745 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770998 Ribosome profiling 15 min after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001746 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770998 Ribosome profiling 15 min after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001746 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770999 Ribosome profiling 8 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001747SRR6001748 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770999 Ribosome profiling 8 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001747SRR6001748 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771000 Ribosome profiling 8 hr after shift to 10°C in ∆cspABEG cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001749SRR6001750 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771000 Ribosome profiling 8 hr after shift to 10°C in ∆cspABEG cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001749SRR6001750 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771001 Ribosome profiling at 37°C in WT cells_2 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001751 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771001 Ribosome profiling at 37°C in WT cells_2 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001751 MCO000003199 37 °C Temperature True
characteristics 35.7 C temperature : <Temp> 37 °C </Temp> GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771001 Ribosome profiling at 37°C in WT cells_2 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt temperature: 37°C PGCGROWTHCONDITIONS SRR6001751 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771002 Ribosome profiling at 37°C in ∆cspABCEG cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001752 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771002 Ribosome profiling at 37°C in ∆cspABCEG cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001752 MCO000003199 37 °C Temperature True
characteristics 35.7 C temperature : <Temp> 37 °C </Temp> GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771002 Ribosome profiling at 37°C in ∆cspABCEG cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt temperature: 37°C PGCGROWTHCONDITIONS SRR6001752 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771008 mRNA-seq 4 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001758 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771008 mRNA-seq 4 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001758 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771010 mRNA-seq 8 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001760SRR6001761 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771010 mRNA-seq 8 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001760SRR6001761 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771011 mRNA-seq 8 hr after shift to 10°C in ∆cspABEG cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001762SRR6001763 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771011 mRNA-seq 8 hr after shift to 10°C in ∆cspABEG cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001762SRR6001763 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771014 DMS-seq 30 min after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001766 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771014 DMS-seq 30 min after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001766 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771015 DMS-seq 6 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001769 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771015 DMS-seq 6 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001769 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771016 DMS-seq 8 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001772 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771016 DMS-seq 8 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001772 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771017 DMS-seq 8 hr after shift to 10°C in ∆cspABEG cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001773 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771017 DMS-seq 8 hr after shift to 10°C in ∆cspABEG cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001773 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771018 DMS-seq 8 hr after shift to 10°C in ∆cspBG cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001774 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771018 DMS-seq 8 hr after shift to 10°C in ∆cspBG cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001774 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771019 Total RNA-seq 20 min after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001775 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771019 Total RNA-seq 20 min after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001775 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771020 Total RNA-seq 4 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001776 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771020 Total RNA-seq 4 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001776 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771021 Total RNA-seq 8 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001777 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771021 Total RNA-seq 8 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001777 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771022 Total RNA-seq 20 min after shift to 10°C in ∆rnr cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001778 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771022 Total RNA-seq 20 min after shift to 10°C in ∆rnr cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001778 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771023 Total RNA-seq 4 hr after shift to 10°C in ∆rnr cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001779 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771023 Total RNA-seq 4 hr after shift to 10°C in ∆rnr cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001779 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771024 Total RNA-seq 8 hr after shift to 10°C in ∆rnr cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001780 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771024 Total RNA-seq 8 hr after shift to 10°C in ∆rnr cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001780 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771025 Total RNA-seq before rifampicin treatment -- 2 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001781 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771025 Total RNA-seq before rifampicin treatment -- 2 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001781 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771026 Total RNA-seq after rifampicin treatment -- 4 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001782 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771026 Total RNA-seq after rifampicin treatment -- 4 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001782 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771027 Total RNA-seq before rifampicin treatment -- 2 hr after shift to 10°C in ∆rnr cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001783 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771027 Total RNA-seq before rifampicin treatment -- 2 hr after shift to 10°C in ∆rnr cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001783 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All culture experiments were performed in <Med> MOPS medium </Med> supplemented with <Supp> 0.2 % glucose </Supp> , 19 amino acids ( without methionine ) , vitamins , bases and micronutrients ( MOPS rich defined medium minus methionine , Teknova ) . Cells were grown in an overnight liquid culture at <Temp> 37 °C </Temp> , diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected . For 10 °C samples , cultures were grown to OD420 = 1.1 at <Temp> 37 °C </Temp> and cold shock was performed by mixing 70mL of <Temp> 37 °C </Temp> culture with 130mL of 0 °C media pre-chilled in ice-water slurry , with continued growth of the culture in a 10 °C shaker . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771028 Total RNA-seq after rifampicin treatment -- 4 hr after shift to 10°C in ∆rnr cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All culture experiments were performed in MOPS medium supplemented with 0.2% glucose, 19 amino acids (without methionine), vitamins, bases and micronutrients (MOPS rich defined medium minus methionine, Teknova). Cells were grown in an overnight liquid culture at 37°C, diluted to an OD420 = .001 in fresh medium and grown until OD420 reached 0.4 where samples were collected. For 10°C samples, cultures were grown to OD420 = 1.1 at 37°C and cold shock was performed by mixing 70mL of 37°C culture with 130mL of 0°C media pre-chilled in ice-water slurry, with continued growth of the culture in a 10°C shaker. PGCGROWTHCONDITIONS SRR6001784 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of E. coli culture was incubated with 750 µL DMS . Incubation was performed for 2 min at <Temp> 37 °C </Temp> or for 45 min at 10 °C . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) , after which cells were quickly put on ice , collected by centrifugation at 8000 x g , 4 °C for 2 min , and washed with 8 mL 30 % BME solution . Cells were then resuspended in 450 µL total RNA lysis buffer ( 10 mM EDTA , 50 mM sodium acetate pH 5.5 ) , and total RNA was purified with hot acid phenol ( Ambion ) . GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771028 Total RNA-seq after rifampicin treatment -- 4 hr after shift to 10°C in ∆rnr cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of E. coli culture was incubated with 750 µL DMS. Incubation was performed for 2 min at 37°C or for 45 min at 10°C. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol), after which cells were quickly put on ice, collected by centrifugation at 8000 x g, 4 °C for 2 min, and washed with 8 mL 30% BME solution. Cells were then resuspended in 450 µL total RNA lysis buffer (10 mM EDTA, 50 mM sodium acetate pH 5.5), and total RNA was purified with hot acid phenol (Ambion). PGCGROWTHCONDITIONS SRR6001784 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cells with construct and control plasmids were grown at <Temp> 37 °C </Temp> overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic . In the morning , 60 μl of each sample were diluted into 3 ml <Med> of fresh medium </Med> and grown at <Temp> 37 °C </Temp> with shaking for another hour ( outgrowth ) . 200 μl of each sample were then transferred in 8 wells of a 96-well plate ( Costar ) at approximately 0.1 OD ( 600 nm ) . The samples were placed in a Synergy HT Microplate Reader ( BioTek ) and incubated at <Temp> 37 °C </Temp> with orbital shaking at 1,000 rpm for 1 h , performing measurements of GFP ( excitation ( ex . ) , 485 nm ; emission ( em . ) , 528 nm ) and OD ( 600 nm ) every 15 minutes . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE107093 Burden- driven feedback control of gene expression GSM2861128 R2 DH10BGFP_pLys_M1_1 29578536 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107093/GSE107093.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cells with construct and control plasmids were grown at 37°C overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic. In the morning, 60 μl of each sample were diluted into 3 ml of fresh medium and grown at 37°C with shaking for another hour (outgrowth). 200 μl of each sample were then transferred in 8 wells of a 96-well plate (Costar) at approximately 0.1 OD (600 nm). The samples were placed in a Synergy HT Microplate Reader (BioTek) and incubated at 37°C with orbital shaking at 1,000 rpm for 1 h, performing measurements of GFP (excitation (ex.), 485 nm; emission (em.), 528 nm) and OD (600 nm) every 15 minutes. PGCGROWTHCONDITIONS SRR6305247 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cells with construct and control plasmids were grown at <Temp> 37 °C </Temp> overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic . In the morning , 60 μl of each sample were diluted into 3 ml <Med> of fresh medium </Med> and grown at <Temp> 37 °C </Temp> with shaking for another hour ( outgrowth ) . 200 μl of each sample were then transferred in 8 wells of a 96-well plate ( Costar ) at approximately 0.1 OD ( 600 nm ) . The samples were placed in a Synergy HT Microplate Reader ( BioTek ) and incubated at <Temp> 37 °C </Temp> with orbital shaking at 1,000 rpm for 1 h , performing measurements of GFP ( excitation ( ex . ) , 485 nm ; emission ( em . ) , 528 nm ) and OD ( 600 nm ) every 15 minutes . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE107093 Burden- driven feedback control of gene expression GSM2861129 R3 DH10BGFP_pSB1C3_1 29578536 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107093/GSE107093.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cells with construct and control plasmids were grown at 37°C overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic. In the morning, 60 μl of each sample were diluted into 3 ml of fresh medium and grown at 37°C with shaking for another hour (outgrowth). 200 μl of each sample were then transferred in 8 wells of a 96-well plate (Costar) at approximately 0.1 OD (600 nm). The samples were placed in a Synergy HT Microplate Reader (BioTek) and incubated at 37°C with orbital shaking at 1,000 rpm for 1 h, performing measurements of GFP (excitation (ex.), 485 nm; emission (em.), 528 nm) and OD (600 nm) every 15 minutes. PGCGROWTHCONDITIONS SRR6305248 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cells with construct and control plasmids were grown at <Temp> 37 °C </Temp> overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic . In the morning , 60 μl of each sample were diluted into 3 ml <Med> of fresh medium </Med> and grown at <Temp> 37 °C </Temp> with shaking for another hour ( outgrowth ) . 200 μl of each sample were then transferred in 8 wells of a 96-well plate ( Costar ) at approximately 0.1 OD ( 600 nm ) . The samples were placed in a Synergy HT Microplate Reader ( BioTek ) and incubated at <Temp> 37 °C </Temp> with orbital shaking at 1,000 rpm for 1 h , performing measurements of GFP ( excitation ( ex . ) , 485 nm ; emission ( em . ) , 528 nm ) and OD ( 600 nm ) every 15 minutes . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE107093 Burden- driven feedback control of gene expression GSM2861132 R6 DH10BGFP_pD864_LacZ_1 29578536 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107093/GSE107093.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cells with construct and control plasmids were grown at 37°C overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic. In the morning, 60 μl of each sample were diluted into 3 ml of fresh medium and grown at 37°C with shaking for another hour (outgrowth). 200 μl of each sample were then transferred in 8 wells of a 96-well plate (Costar) at approximately 0.1 OD (600 nm). The samples were placed in a Synergy HT Microplate Reader (BioTek) and incubated at 37°C with orbital shaking at 1,000 rpm for 1 h, performing measurements of GFP (excitation (ex.), 485 nm; emission (em.), 528 nm) and OD (600 nm) every 15 minutes. PGCGROWTHCONDITIONS SRR6305251 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cells with construct and control plasmids were grown at <Temp> 37 °C </Temp> overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic . In the morning , 60 μl of each sample were diluted into 3 ml <Med> of fresh medium </Med> and grown at <Temp> 37 °C </Temp> with shaking for another hour ( outgrowth ) . 200 μl of each sample were then transferred in 8 wells of a 96-well plate ( Costar ) at approximately 0.1 OD ( 600 nm ) . The samples were placed in a Synergy HT Microplate Reader ( BioTek ) and incubated at <Temp> 37 °C </Temp> with orbital shaking at 1,000 rpm for 1 h , performing measurements of GFP ( excitation ( ex . ) , 485 nm ; emission ( em . ) , 528 nm ) and OD ( 600 nm ) every 15 minutes . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE107093 Burden- driven feedback control of gene expression GSM2861137 R11 DH10BGFP_pLys_1 29578536 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107093/GSE107093.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cells with construct and control plasmids were grown at 37°C overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic. In the morning, 60 μl of each sample were diluted into 3 ml of fresh medium and grown at 37°C with shaking for another hour (outgrowth). 200 μl of each sample were then transferred in 8 wells of a 96-well plate (Costar) at approximately 0.1 OD (600 nm). The samples were placed in a Synergy HT Microplate Reader (BioTek) and incubated at 37°C with orbital shaking at 1,000 rpm for 1 h, performing measurements of GFP (excitation (ex.), 485 nm; emission (em.), 528 nm) and OD (600 nm) every 15 minutes. PGCGROWTHCONDITIONS SRR6305256 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cells with construct and control plasmids were grown at <Temp> 37 °C </Temp> overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic . In the morning , 60 μl of each sample were diluted into 3 ml <Med> of fresh medium </Med> and grown at <Temp> 37 °C </Temp> with shaking for another hour ( outgrowth ) . 200 μl of each sample were then transferred in 8 wells of a 96-well plate ( Costar ) at approximately 0.1 OD ( 600 nm ) . The samples were placed in a Synergy HT Microplate Reader ( BioTek ) and incubated at <Temp> 37 °C </Temp> with orbital shaking at 1,000 rpm for 1 h , performing measurements of GFP ( excitation ( ex . ) , 485 nm ; emission ( em . ) , 528 nm ) and OD ( 600 nm ) every 15 minutes . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE107093 Burden- driven feedback control of gene expression GSM2861142 R16 MG1655GFP_pLys_M1_1 29578536 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107093/GSE107093.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cells with construct and control plasmids were grown at 37°C overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic. In the morning, 60 μl of each sample were diluted into 3 ml of fresh medium and grown at 37°C with shaking for another hour (outgrowth). 200 μl of each sample were then transferred in 8 wells of a 96-well plate (Costar) at approximately 0.1 OD (600 nm). The samples were placed in a Synergy HT Microplate Reader (BioTek) and incubated at 37°C with orbital shaking at 1,000 rpm for 1 h, performing measurements of GFP (excitation (ex.), 485 nm; emission (em.), 528 nm) and OD (600 nm) every 15 minutes. PGCGROWTHCONDITIONS SRR6305261 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cells with construct and control plasmids were grown at <Temp> 37 °C </Temp> overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic . In the morning , 60 μl of each sample were diluted into 3 ml <Med> of fresh medium </Med> and grown at <Temp> 37 °C </Temp> with shaking for another hour ( outgrowth ) . 200 μl of each sample were then transferred in 8 wells of a 96-well plate ( Costar ) at approximately 0.1 OD ( 600 nm ) . The samples were placed in a Synergy HT Microplate Reader ( BioTek ) and incubated at <Temp> 37 °C </Temp> with orbital shaking at 1,000 rpm for 1 h , performing measurements of GFP ( excitation ( ex . ) , 485 nm ; emission ( em . ) , 528 nm ) and OD ( 600 nm ) every 15 minutes . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE107093 Burden- driven feedback control of gene expression GSM2861145 R19 MG1655GFP_Lux_1 29578536 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107093/GSE107093.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cells with construct and control plasmids were grown at 37°C overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic. In the morning, 60 μl of each sample were diluted into 3 ml of fresh medium and grown at 37°C with shaking for another hour (outgrowth). 200 μl of each sample were then transferred in 8 wells of a 96-well plate (Costar) at approximately 0.1 OD (600 nm). The samples were placed in a Synergy HT Microplate Reader (BioTek) and incubated at 37°C with orbital shaking at 1,000 rpm for 1 h, performing measurements of GFP (excitation (ex.), 485 nm; emission (em.), 528 nm) and OD (600 nm) every 15 minutes. PGCGROWTHCONDITIONS SRR6305264 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cells with construct and control plasmids were grown at <Temp> 37 °C </Temp> overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic . In the morning , 60 μl of each sample were diluted into 3 ml <Med> of fresh medium </Med> and grown at <Temp> 37 °C </Temp> with shaking for another hour ( outgrowth ) . 200 μl of each sample were then transferred in 8 wells of a 96-well plate ( Costar ) at approximately 0.1 OD ( 600 nm ) . The samples were placed in a Synergy HT Microplate Reader ( BioTek ) and incubated at <Temp> 37 °C </Temp> with orbital shaking at 1,000 rpm for 1 h , performing measurements of GFP ( excitation ( ex . ) , 485 nm ; emission ( em . ) , 528 nm ) and OD ( 600 nm ) every 15 minutes . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE107093 Burden- driven feedback control of gene expression GSM2861152 R26 MG1655GFP_Lux_1 29578536 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107093/GSE107093.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cells with construct and control plasmids were grown at 37°C overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic. In the morning, 60 μl of each sample were diluted into 3 ml of fresh medium and grown at 37°C with shaking for another hour (outgrowth). 200 μl of each sample were then transferred in 8 wells of a 96-well plate (Costar) at approximately 0.1 OD (600 nm). The samples were placed in a Synergy HT Microplate Reader (BioTek) and incubated at 37°C with orbital shaking at 1,000 rpm for 1 h, performing measurements of GFP (excitation (ex.), 485 nm; emission (em.), 528 nm) and OD (600 nm) every 15 minutes. PGCGROWTHCONDITIONS SRR6305271 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cells with construct and control plasmids were grown at <Temp> 37 °C </Temp> overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic . In the morning , 60 μl of each sample were diluted into 3 ml <Med> of fresh medium </Med> and grown at <Temp> 37 °C </Temp> with shaking for another hour ( outgrowth ) . 200 μl of each sample were then transferred in 8 wells of a 96-well plate ( Costar ) at approximately 0.1 OD ( 600 nm ) . The samples were placed in a Synergy HT Microplate Reader ( BioTek ) and incubated at <Temp> 37 °C </Temp> with orbital shaking at 1,000 rpm for 1 h , performing measurements of GFP ( excitation ( ex . ) , 485 nm ; emission ( em . ) , 528 nm ) and OD ( 600 nm ) every 15 minutes . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE107093 Burden- driven feedback control of gene expression GSM2861153 R27 MG1655GFP_pD864_LacZ_1 29578536 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107093/GSE107093.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cells with construct and control plasmids were grown at 37°C overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic. In the morning, 60 μl of each sample were diluted into 3 ml of fresh medium and grown at 37°C with shaking for another hour (outgrowth). 200 μl of each sample were then transferred in 8 wells of a 96-well plate (Costar) at approximately 0.1 OD (600 nm). The samples were placed in a Synergy HT Microplate Reader (BioTek) and incubated at 37°C with orbital shaking at 1,000 rpm for 1 h, performing measurements of GFP (excitation (ex.), 485 nm; emission (em.), 528 nm) and OD (600 nm) every 15 minutes. PGCGROWTHCONDITIONS SRR6305272 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cells with construct and control plasmids were grown at <Temp> 37 °C </Temp> overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic . In the morning , 60 μl of each sample were diluted into 3 ml <Med> of fresh medium </Med> and grown at <Temp> 37 °C </Temp> with shaking for another hour ( outgrowth ) . 200 μl of each sample were then transferred in 8 wells of a 96-well plate ( Costar ) at approximately 0.1 OD ( 600 nm ) . The samples were placed in a Synergy HT Microplate Reader ( BioTek ) and incubated at <Temp> 37 °C </Temp> with orbital shaking at 1,000 rpm for 1 h , performing measurements of GFP ( excitation ( ex . ) , 485 nm ; emission ( em . ) , 528 nm ) and OD ( 600 nm ) every 15 minutes . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE107093 Burden- driven feedback control of gene expression GSM2861155 R29 DH10BGFP_None_1 29578536 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107093/GSE107093.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cells with construct and control plasmids were grown at 37°C overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic. In the morning, 60 μl of each sample were diluted into 3 ml of fresh medium and grown at 37°C with shaking for another hour (outgrowth). 200 μl of each sample were then transferred in 8 wells of a 96-well plate (Costar) at approximately 0.1 OD (600 nm). The samples were placed in a Synergy HT Microplate Reader (BioTek) and incubated at 37°C with orbital shaking at 1,000 rpm for 1 h, performing measurements of GFP (excitation (ex.), 485 nm; emission (em.), 528 nm) and OD (600 nm) every 15 minutes. PGCGROWTHCONDITIONS SRR6305274 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cells with construct and control plasmids were grown at <Temp> 37 °C </Temp> overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic . In the morning , 60 μl of each sample were diluted into 3 ml <Med> of fresh medium </Med> and grown at <Temp> 37 °C </Temp> with shaking for another hour ( outgrowth ) . 200 μl of each sample were then transferred in 8 wells of a 96-well plate ( Costar ) at approximately 0.1 OD ( 600 nm ) . The samples were placed in a Synergy HT Microplate Reader ( BioTek ) and incubated at <Temp> 37 °C </Temp> with orbital shaking at 1,000 rpm for 1 h , performing measurements of GFP ( excitation ( ex . ) , 485 nm ; emission ( em . ) , 528 nm ) and OD ( 600 nm ) every 15 minutes . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE107093 Burden- driven feedback control of gene expression GSM2861159 B3 DH10BGFP_pSB1C3_2 29578536 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107093/GSE107093.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cells with construct and control plasmids were grown at 37°C overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic. In the morning, 60 μl of each sample were diluted into 3 ml of fresh medium and grown at 37°C with shaking for another hour (outgrowth). 200 μl of each sample were then transferred in 8 wells of a 96-well plate (Costar) at approximately 0.1 OD (600 nm). The samples were placed in a Synergy HT Microplate Reader (BioTek) and incubated at 37°C with orbital shaking at 1,000 rpm for 1 h, performing measurements of GFP (excitation (ex.), 485 nm; emission (em.), 528 nm) and OD (600 nm) every 15 minutes. PGCGROWTHCONDITIONS SRR6305278 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cells with construct and control plasmids were grown at <Temp> 37 °C </Temp> overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic . In the morning , 60 μl of each sample were diluted into 3 ml <Med> of fresh medium </Med> and grown at <Temp> 37 °C </Temp> with shaking for another hour ( outgrowth ) . 200 μl of each sample were then transferred in 8 wells of a 96-well plate ( Costar ) at approximately 0.1 OD ( 600 nm ) . The samples were placed in a Synergy HT Microplate Reader ( BioTek ) and incubated at <Temp> 37 °C </Temp> with orbital shaking at 1,000 rpm for 1 h , performing measurements of GFP ( excitation ( ex . ) , 485 nm ; emission ( em . ) , 528 nm ) and OD ( 600 nm ) every 15 minutes . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE107093 Burden- driven feedback control of gene expression GSM2861160 B4 DH10BGFP_pLys_2 29578536 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107093/GSE107093.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cells with construct and control plasmids were grown at 37°C overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic. In the morning, 60 μl of each sample were diluted into 3 ml of fresh medium and grown at 37°C with shaking for another hour (outgrowth). 200 μl of each sample were then transferred in 8 wells of a 96-well plate (Costar) at approximately 0.1 OD (600 nm). The samples were placed in a Synergy HT Microplate Reader (BioTek) and incubated at 37°C with orbital shaking at 1,000 rpm for 1 h, performing measurements of GFP (excitation (ex.), 485 nm; emission (em.), 528 nm) and OD (600 nm) every 15 minutes. PGCGROWTHCONDITIONS SRR6305279 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cells with construct and control plasmids were grown at <Temp> 37 °C </Temp> overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic . In the morning , 60 μl of each sample were diluted into 3 ml <Med> of fresh medium </Med> and grown at <Temp> 37 °C </Temp> with shaking for another hour ( outgrowth ) . 200 μl of each sample were then transferred in 8 wells of a 96-well plate ( Costar ) at approximately 0.1 OD ( 600 nm ) . The samples were placed in a Synergy HT Microplate Reader ( BioTek ) and incubated at <Temp> 37 °C </Temp> with orbital shaking at 1,000 rpm for 1 h , performing measurements of GFP ( excitation ( ex . ) , 485 nm ; emission ( em . ) , 528 nm ) and OD ( 600 nm ) every 15 minutes . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE107093 Burden- driven feedback control of gene expression GSM2861163 B7 DH10BGFP_pD864_2 29578536 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107093/GSE107093.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cells with construct and control plasmids were grown at 37°C overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic. In the morning, 60 μl of each sample were diluted into 3 ml of fresh medium and grown at 37°C with shaking for another hour (outgrowth). 200 μl of each sample were then transferred in 8 wells of a 96-well plate (Costar) at approximately 0.1 OD (600 nm). The samples were placed in a Synergy HT Microplate Reader (BioTek) and incubated at 37°C with orbital shaking at 1,000 rpm for 1 h, performing measurements of GFP (excitation (ex.), 485 nm; emission (em.), 528 nm) and OD (600 nm) every 15 minutes. PGCGROWTHCONDITIONS SRR6305282 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cells with construct and control plasmids were grown at <Temp> 37 °C </Temp> overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic . In the morning , 60 μl of each sample were diluted into 3 ml <Med> of fresh medium </Med> and grown at <Temp> 37 °C </Temp> with shaking for another hour ( outgrowth ) . 200 μl of each sample were then transferred in 8 wells of a 96-well plate ( Costar ) at approximately 0.1 OD ( 600 nm ) . The samples were placed in a Synergy HT Microplate Reader ( BioTek ) and incubated at <Temp> 37 °C </Temp> with orbital shaking at 1,000 rpm for 1 h , performing measurements of GFP ( excitation ( ex . ) , 485 nm ; emission ( em . ) , 528 nm ) and OD ( 600 nm ) every 15 minutes . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE107093 Burden- driven feedback control of gene expression GSM2861165 B9 DH10BGFP_pLys_M1_2 29578536 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107093/GSE107093.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cells with construct and control plasmids were grown at 37°C overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic. In the morning, 60 μl of each sample were diluted into 3 ml of fresh medium and grown at 37°C with shaking for another hour (outgrowth). 200 μl of each sample were then transferred in 8 wells of a 96-well plate (Costar) at approximately 0.1 OD (600 nm). The samples were placed in a Synergy HT Microplate Reader (BioTek) and incubated at 37°C with orbital shaking at 1,000 rpm for 1 h, performing measurements of GFP (excitation (ex.), 485 nm; emission (em.), 528 nm) and OD (600 nm) every 15 minutes. PGCGROWTHCONDITIONS SRR6305284 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cells with construct and control plasmids were grown at <Temp> 37 °C </Temp> overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic . In the morning , 60 μl of each sample were diluted into 3 ml <Med> of fresh medium </Med> and grown at <Temp> 37 °C </Temp> with shaking for another hour ( outgrowth ) . 200 μl of each sample were then transferred in 8 wells of a 96-well plate ( Costar ) at approximately 0.1 OD ( 600 nm ) . The samples were placed in a Synergy HT Microplate Reader ( BioTek ) and incubated at <Temp> 37 °C </Temp> with orbital shaking at 1,000 rpm for 1 h , performing measurements of GFP ( excitation ( ex . ) , 485 nm ; emission ( em . ) , 528 nm ) and OD ( 600 nm ) every 15 minutes . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE107093 Burden- driven feedback control of gene expression GSM2861166 B10 DH10BGFP_pSB1C3_2 29578536 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107093/GSE107093.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cells with construct and control plasmids were grown at 37°C overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic. In the morning, 60 μl of each sample were diluted into 3 ml of fresh medium and grown at 37°C with shaking for another hour (outgrowth). 200 μl of each sample were then transferred in 8 wells of a 96-well plate (Costar) at approximately 0.1 OD (600 nm). The samples were placed in a Synergy HT Microplate Reader (BioTek) and incubated at 37°C with orbital shaking at 1,000 rpm for 1 h, performing measurements of GFP (excitation (ex.), 485 nm; emission (em.), 528 nm) and OD (600 nm) every 15 minutes. PGCGROWTHCONDITIONS SRR6305285 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cells with construct and control plasmids were grown at <Temp> 37 °C </Temp> overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic . In the morning , 60 μl of each sample were diluted into 3 ml <Med> of fresh medium </Med> and grown at <Temp> 37 °C </Temp> with shaking for another hour ( outgrowth ) . 200 μl of each sample were then transferred in 8 wells of a 96-well plate ( Costar ) at approximately 0.1 OD ( 600 nm ) . The samples were placed in a Synergy HT Microplate Reader ( BioTek ) and incubated at <Temp> 37 °C </Temp> with orbital shaking at 1,000 rpm for 1 h , performing measurements of GFP ( excitation ( ex . ) , 485 nm ; emission ( em . ) , 528 nm ) and OD ( 600 nm ) every 15 minutes . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE107093 Burden- driven feedback control of gene expression GSM2861173 B17 MG1655GFP_pSB1C3_2 29578536 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107093/GSE107093.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cells with construct and control plasmids were grown at 37°C overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic. In the morning, 60 μl of each sample were diluted into 3 ml of fresh medium and grown at 37°C with shaking for another hour (outgrowth). 200 μl of each sample were then transferred in 8 wells of a 96-well plate (Costar) at approximately 0.1 OD (600 nm). The samples were placed in a Synergy HT Microplate Reader (BioTek) and incubated at 37°C with orbital shaking at 1,000 rpm for 1 h, performing measurements of GFP (excitation (ex.), 485 nm; emission (em.), 528 nm) and OD (600 nm) every 15 minutes. PGCGROWTHCONDITIONS SRR6305292 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cells with construct and control plasmids were grown at <Temp> 37 °C </Temp> overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic . In the morning , 60 μl of each sample were diluted into 3 ml <Med> of fresh medium </Med> and grown at <Temp> 37 °C </Temp> with shaking for another hour ( outgrowth ) . 200 μl of each sample were then transferred in 8 wells of a 96-well plate ( Costar ) at approximately 0.1 OD ( 600 nm ) . The samples were placed in a Synergy HT Microplate Reader ( BioTek ) and incubated at <Temp> 37 °C </Temp> with orbital shaking at 1,000 rpm for 1 h , performing measurements of GFP ( excitation ( ex . ) , 485 nm ; emission ( em . ) , 528 nm ) and OD ( 600 nm ) every 15 minutes . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE107093 Burden- driven feedback control of gene expression GSM2861178 B22 MG1655GFP_pSB1C3_H3_2 29578536 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107093/GSE107093.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cells with construct and control plasmids were grown at 37°C overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic. In the morning, 60 μl of each sample were diluted into 3 ml of fresh medium and grown at 37°C with shaking for another hour (outgrowth). 200 μl of each sample were then transferred in 8 wells of a 96-well plate (Costar) at approximately 0.1 OD (600 nm). The samples were placed in a Synergy HT Microplate Reader (BioTek) and incubated at 37°C with orbital shaking at 1,000 rpm for 1 h, performing measurements of GFP (excitation (ex.), 485 nm; emission (em.), 528 nm) and OD (600 nm) every 15 minutes. PGCGROWTHCONDITIONS SRR6305297 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cells with construct and control plasmids were grown at <Temp> 37 °C </Temp> overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic . In the morning , 60 μl of each sample were diluted into 3 ml <Med> of fresh medium </Med> and grown at <Temp> 37 °C </Temp> with shaking for another hour ( outgrowth ) . 200 μl of each sample were then transferred in 8 wells of a 96-well plate ( Costar ) at approximately 0.1 OD ( 600 nm ) . The samples were placed in a Synergy HT Microplate Reader ( BioTek ) and incubated at <Temp> 37 °C </Temp> with orbital shaking at 1,000 rpm for 1 h , performing measurements of GFP ( excitation ( ex . ) , 485 nm ; emission ( em . ) , 528 nm ) and OD ( 600 nm ) every 15 minutes . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE107093 Burden- driven feedback control of gene expression GSM2861182 B26 MG1655GFP_Lux_2 29578536 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107093/GSE107093.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cells with construct and control plasmids were grown at 37°C overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic. In the morning, 60 μl of each sample were diluted into 3 ml of fresh medium and grown at 37°C with shaking for another hour (outgrowth). 200 μl of each sample were then transferred in 8 wells of a 96-well plate (Costar) at approximately 0.1 OD (600 nm). The samples were placed in a Synergy HT Microplate Reader (BioTek) and incubated at 37°C with orbital shaking at 1,000 rpm for 1 h, performing measurements of GFP (excitation (ex.), 485 nm; emission (em.), 528 nm) and OD (600 nm) every 15 minutes. PGCGROWTHCONDITIONS SRR6305301 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cells with construct and control plasmids were grown at <Temp> 37 °C </Temp> overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic . In the morning , 60 μl of each sample were diluted into 3 ml <Med> of fresh medium </Med> and grown at <Temp> 37 °C </Temp> with shaking for another hour ( outgrowth ) . 200 μl of each sample were then transferred in 8 wells of a 96-well plate ( Costar ) at approximately 0.1 OD ( 600 nm ) . The samples were placed in a Synergy HT Microplate Reader ( BioTek ) and incubated at <Temp> 37 °C </Temp> with orbital shaking at 1,000 rpm for 1 h , performing measurements of GFP ( excitation ( ex . ) , 485 nm ; emission ( em . ) , 528 nm ) and OD ( 600 nm ) every 15 minutes . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE107093 Burden- driven feedback control of gene expression GSM2861191 G5 DH10BGFP_Lux_3 29578536 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107093/GSE107093.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cells with construct and control plasmids were grown at 37°C overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic. In the morning, 60 μl of each sample were diluted into 3 ml of fresh medium and grown at 37°C with shaking for another hour (outgrowth). 200 μl of each sample were then transferred in 8 wells of a 96-well plate (Costar) at approximately 0.1 OD (600 nm). The samples were placed in a Synergy HT Microplate Reader (BioTek) and incubated at 37°C with orbital shaking at 1,000 rpm for 1 h, performing measurements of GFP (excitation (ex.), 485 nm; emission (em.), 528 nm) and OD (600 nm) every 15 minutes. PGCGROWTHCONDITIONS SRR6305310 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cells with construct and control plasmids were grown at <Temp> 37 °C </Temp> overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic . In the morning , 60 μl of each sample were diluted into 3 ml <Med> of fresh medium </Med> and grown at <Temp> 37 °C </Temp> with shaking for another hour ( outgrowth ) . 200 μl of each sample were then transferred in 8 wells of a 96-well plate ( Costar ) at approximately 0.1 OD ( 600 nm ) . The samples were placed in a Synergy HT Microplate Reader ( BioTek ) and incubated at <Temp> 37 °C </Temp> with orbital shaking at 1,000 rpm for 1 h , performing measurements of GFP ( excitation ( ex . ) , 485 nm ; emission ( em . ) , 528 nm ) and OD ( 600 nm ) every 15 minutes . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE107093 Burden- driven feedback control of gene expression GSM2861192 G6 DH10BGFP_pD864_LacZ_3 29578536 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107093/GSE107093.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cells with construct and control plasmids were grown at 37°C overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic. In the morning, 60 μl of each sample were diluted into 3 ml of fresh medium and grown at 37°C with shaking for another hour (outgrowth). 200 μl of each sample were then transferred in 8 wells of a 96-well plate (Costar) at approximately 0.1 OD (600 nm). The samples were placed in a Synergy HT Microplate Reader (BioTek) and incubated at 37°C with orbital shaking at 1,000 rpm for 1 h, performing measurements of GFP (excitation (ex.), 485 nm; emission (em.), 528 nm) and OD (600 nm) every 15 minutes. PGCGROWTHCONDITIONS SRR6305311 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cells with construct and control plasmids were grown at <Temp> 37 °C </Temp> overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic . In the morning , 60 μl of each sample were diluted into 3 ml <Med> of fresh medium </Med> and grown at <Temp> 37 °C </Temp> with shaking for another hour ( outgrowth ) . 200 μl of each sample were then transferred in 8 wells of a 96-well plate ( Costar ) at approximately 0.1 OD ( 600 nm ) . The samples were placed in a Synergy HT Microplate Reader ( BioTek ) and incubated at <Temp> 37 °C </Temp> with orbital shaking at 1,000 rpm for 1 h , performing measurements of GFP ( excitation ( ex . ) , 485 nm ; emission ( em . ) , 528 nm ) and OD ( 600 nm ) every 15 minutes . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE107093 Burden- driven feedback control of gene expression GSM2861207 G21 MG1655GFP_pD864_3 29578536 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107093/GSE107093.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cells with construct and control plasmids were grown at 37°C overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic. In the morning, 60 μl of each sample were diluted into 3 ml of fresh medium and grown at 37°C with shaking for another hour (outgrowth). 200 μl of each sample were then transferred in 8 wells of a 96-well plate (Costar) at approximately 0.1 OD (600 nm). The samples were placed in a Synergy HT Microplate Reader (BioTek) and incubated at 37°C with orbital shaking at 1,000 rpm for 1 h, performing measurements of GFP (excitation (ex.), 485 nm; emission (em.), 528 nm) and OD (600 nm) every 15 minutes. PGCGROWTHCONDITIONS SRR6305326 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cells with construct and control plasmids were grown at <Temp> 37 °C </Temp> overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic . In the morning , 60 μl of each sample were diluted into 3 ml <Med> of fresh medium </Med> and grown at <Temp> 37 °C </Temp> with shaking for another hour ( outgrowth ) . 200 μl of each sample were then transferred in 8 wells of a 96-well plate ( Costar ) at approximately 0.1 OD ( 600 nm ) . The samples were placed in a Synergy HT Microplate Reader ( BioTek ) and incubated at <Temp> 37 °C </Temp> with orbital shaking at 1,000 rpm for 1 h , performing measurements of GFP ( excitation ( ex . ) , 485 nm ; emission ( em . ) , 528 nm ) and OD ( 600 nm ) every 15 minutes . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE107093 Burden- driven feedback control of gene expression GSM2861209 G23 MG1655GFP_pLys_M1_3 29578536 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107093/GSE107093.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cells with construct and control plasmids were grown at 37°C overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic. In the morning, 60 μl of each sample were diluted into 3 ml of fresh medium and grown at 37°C with shaking for another hour (outgrowth). 200 μl of each sample were then transferred in 8 wells of a 96-well plate (Costar) at approximately 0.1 OD (600 nm). The samples were placed in a Synergy HT Microplate Reader (BioTek) and incubated at 37°C with orbital shaking at 1,000 rpm for 1 h, performing measurements of GFP (excitation (ex.), 485 nm; emission (em.), 528 nm) and OD (600 nm) every 15 minutes. PGCGROWTHCONDITIONS SRR6305328 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cells with construct and control plasmids were grown at <Temp> 37 °C </Temp> overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic . In the morning , 60 μl of each sample were diluted into 3 ml <Med> of fresh medium </Med> and grown at <Temp> 37 °C </Temp> with shaking for another hour ( outgrowth ) . 200 μl of each sample were then transferred in 8 wells of a 96-well plate ( Costar ) at approximately 0.1 OD ( 600 nm ) . The samples were placed in a Synergy HT Microplate Reader ( BioTek ) and incubated at <Temp> 37 °C </Temp> with orbital shaking at 1,000 rpm for 1 h , performing measurements of GFP ( excitation ( ex . ) , 485 nm ; emission ( em . ) , 528 nm ) and OD ( 600 nm ) every 15 minutes . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE107093 Burden- driven feedback control of gene expression GSM2861214 G28 MG1655GFP_pD864_3 29578536 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107093/GSE107093.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cells with construct and control plasmids were grown at 37°C overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic. In the morning, 60 μl of each sample were diluted into 3 ml of fresh medium and grown at 37°C with shaking for another hour (outgrowth). 200 μl of each sample were then transferred in 8 wells of a 96-well plate (Costar) at approximately 0.1 OD (600 nm). The samples were placed in a Synergy HT Microplate Reader (BioTek) and incubated at 37°C with orbital shaking at 1,000 rpm for 1 h, performing measurements of GFP (excitation (ex.), 485 nm; emission (em.), 528 nm) and OD (600 nm) every 15 minutes. PGCGROWTHCONDITIONS SRR6305333 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cells with construct and control plasmids were grown at <Temp> 37 °C </Temp> overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic . In the morning , 60 μl of each sample were diluted into 3 ml <Med> of fresh medium </Med> and grown at <Temp> 37 °C </Temp> with shaking for another hour ( outgrowth ) . 200 μl of each sample were then transferred in 8 wells of a 96-well plate ( Costar ) at approximately 0.1 OD ( 600 nm ) . The samples were placed in a Synergy HT Microplate Reader ( BioTek ) and incubated at <Temp> 37 °C </Temp> with orbital shaking at 1,000 rpm for 1 h , performing measurements of GFP ( excitation ( ex . ) , 485 nm ; emission ( em . ) , 528 nm ) and OD ( 600 nm ) every 15 minutes . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE107093 Burden- driven feedback control of gene expression GSM2861215 G29 DH10BGFP_None_3 29578536 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107093/GSE107093.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cells with construct and control plasmids were grown at 37°C overnight with aeration in a shaking incubator in 5 ml of defined supplemented M9 medium with the appropriate antibiotic. In the morning, 60 μl of each sample were diluted into 3 ml of fresh medium and grown at 37°C with shaking for another hour (outgrowth). 200 μl of each sample were then transferred in 8 wells of a 96-well plate (Costar) at approximately 0.1 OD (600 nm). The samples were placed in a Synergy HT Microplate Reader (BioTek) and incubated at 37°C with orbital shaking at 1,000 rpm for 1 h, performing measurements of GFP (excitation (ex.), 485 nm; emission (em.), 528 nm) and OD (600 nm) every 15 minutes. PGCGROWTHCONDITIONS SRR6305334 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C For 3C-seq : ≈ 1-2 x 109 crosslinked cells ( 7 % formaldehyde , unless otherwise stated ) are suspended in 600 μl Tris 10 mM EDTA 0.5 mM ( TE ) ( pH 8 ) with 4 μl of lysozyme ( 35 U/μl ; Tebu Bio ) , and incubated at RT for 20 minutes . SDS is added to the mix ( final concentration 0.5 % ) and the cells are incubated for 10 minutes at RT. 50μl of lysed cells are then transferred in 8 tubes containing 450μL of digestion mix ( 1X NEB 1 buffer , 1 % triton X-100 , and 100U HpaII enzyme ) . DNA is digested for 3 hours at <Temp> 37 °C </Temp> , split in 4 aliquots , and diluted in 8 ml ligation buffer ( 1X ligation buffer NEB without ATP , 1 mM ATP , 0.1 mg/ml BSA , 125 Units of T4 DNA ligase 5 U/μl ) . Ligation is then performed at 16 °C for 4 hours , followed by incubation overnight ( ON ) at 65 °C in presence of 250 μg/ml proteinase K and 5 mM EDTA . Precipitation of DNA is performed using an equal volume of 3 M Na-Acetate ( pH 5.2 ) and two volumes of iso-propanol . After one hour at -80 °C , DNA is pelleted , suspended in 500μl 1X TE buffer , and incubated for 30 minutes at <Temp> 37 °C </Temp> in presence of RNAse A ( 0.03 mg/ml ) . DNA is then transferred into 2 ml centrifuge tubes , extracted twice with 500 μl phenol-chloroform pH 8.0 , precipitated , washed with 1 ml cold ethanol 70 % and diluted in 30 μl 1X TE buffer . All tubes are pooled and the resulting 3C library is quantified on gel using Quantity One software ( BioRad ) . GPL15010-GPL21117 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL21117: Illumina NextSeq 500 (Escherichia coli str. K-12 substr. MG1655) GSE107301 Multiscale structuring of the E. coli chromosome by nucleoid-associated and condensin proteins GSM2870440 RNA-seq of E. coli wt cells in stationary phase 30°C (30h) 29358050 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107301/GSE107301.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For 3C-seq: ≈ 1-2 x 109 crosslinked cells (7% formaldehyde, unless otherwise stated ) are suspended in 600 μl Tris 10 mM EDTA 0.5 mM (TE) (pH 8) with 4 μl of lysozyme (35 U/μl; Tebu Bio), and incubated at RT for 20 minutes. SDS is added to the mix (final concentration 0.5%) and the cells are incubated for 10 minutes at RT. 50μl of lysed cells are then transferred in 8 tubes containing 450μL of digestion mix (1X NEB 1 buffer, 1% triton X-100, and 100U HpaII enzyme). DNA is digested for 3 hours at 37°C, split in 4 aliquots, and diluted in 8 ml ligation buffer (1X ligation buffer NEB without ATP, 1 mM ATP, 0.1 mg/ml BSA, 125 Units of T4 DNA ligase 5 U/μl). Ligation is then performed at 16°C for 4 hours, followed by incubation overnight (ON) at 65°C in presence of 250 μg/ml proteinase K and 5 mM EDTA. Precipitation of DNA is performed using an equal volume of 3 M Na-Acetate (pH 5.2) and two volumes of iso-propanol. After one hour at -80°C, DNA is pelleted, suspended in 500μl 1X TE buffer, and incubated for 30 minutes at 37°C in presence of RNAse A (0.03 mg/ml). DNA is then transferred into 2 ml centrifuge tubes, extracted twice with 500 μl phenol-chloroform pH 8.0, precipitated, washed with 1 ml cold ethanol 70% and diluted in 30 μl 1X TE buffer. All tubes are pooled and the resulting 3C library is quantified on gel using Quantity One software (BioRad). PGCGROWTHCONDITIONS SRR6354578 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cells were grown at 22 °C , 30 °C and <Temp> 37 °C </Temp> in either Lennox Broth ( LB ) or liquid minimal medium A supplemented with 0.12 % casamino acids and 0.4 % glucose . The cultures were grown to OD600 = 0.2 ( early exponential ) or 2 ( stationary phase ) . GPL15010-GPL21117 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL21117: Illumina NextSeq 500 (Escherichia coli str. K-12 substr. MG1655) GSE107301 Multiscale structuring of the E. coli chromosome by nucleoid-associated and condensin proteins GSM2870440 RNA-seq of E. coli wt cells in stationary phase 30°C (30h) 29358050 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107301/GSE107301.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cells were grown at 22°C, 30°C and 37°C in either Lennox Broth (LB) or liquid minimal medium A supplemented with 0.12% casamino acids and 0.4% glucose. The cultures were grown to OD600 = 0.2 (early exponential) or 2 (stationary phase). PGCGROWTHCONDITIONS SRR6354578 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C For 3C-seq : ≈ 1-2 x 109 crosslinked cells ( 7 % formaldehyde , unless otherwise stated ) are suspended in 600 μl Tris 10 mM EDTA 0.5 mM ( TE ) ( pH 8 ) with 4 μl of lysozyme ( 35 U/μl ; Tebu Bio ) , and incubated at RT for 20 minutes . SDS is added to the mix ( final concentration 0.5 % ) and the cells are incubated for 10 minutes at RT. 50μl of lysed cells are then transferred in 8 tubes containing 450μL of digestion mix ( 1X NEB 1 buffer , 1 % triton X-100 , and 100U HpaII enzyme ) . DNA is digested for 3 hours at <Temp> 37 °C </Temp> , split in 4 aliquots , and diluted in 8 ml ligation buffer ( 1X ligation buffer NEB without ATP , 1 mM ATP , 0.1 mg/ml BSA , 125 Units of T4 DNA ligase 5 U/μl ) . Ligation is then performed at 16 °C for 4 hours , followed by incubation overnight ( ON ) at 65 °C in presence of 250 μg/ml proteinase K and 5 mM EDTA . Precipitation of DNA is performed using an equal volume of 3 M Na-Acetate ( pH 5.2 ) and two volumes of iso-propanol . After one hour at -80 °C , DNA is pelleted , suspended in 500μl 1X TE buffer , and incubated for 30 minutes at <Temp> 37 °C </Temp> in presence of RNAse A ( 0.03 mg/ml ) . DNA is then transferred into 2 ml centrifuge tubes , extracted twice with 500 μl phenol-chloroform pH 8.0 , precipitated , washed with 1 ml cold ethanol 70 % and diluted in 30 μl 1X TE buffer . All tubes are pooled and the resulting 3C library is quantified on gel using Quantity One software ( BioRad ) . GPL15010-GPL21117 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL21117: Illumina NextSeq 500 (Escherichia coli str. K-12 substr. MG1655) GSE107301 Multiscale structuring of the E. coli chromosome by nucleoid-associated and condensin proteins GSM2870441 RNA-seq of E. coli wt cells in stationary phase 30°C (30h) -Replicate 1 29358050 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107301/GSE107301.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For 3C-seq: ≈ 1-2 x 109 crosslinked cells (7% formaldehyde, unless otherwise stated ) are suspended in 600 μl Tris 10 mM EDTA 0.5 mM (TE) (pH 8) with 4 μl of lysozyme (35 U/μl; Tebu Bio), and incubated at RT for 20 minutes. SDS is added to the mix (final concentration 0.5%) and the cells are incubated for 10 minutes at RT. 50μl of lysed cells are then transferred in 8 tubes containing 450μL of digestion mix (1X NEB 1 buffer, 1% triton X-100, and 100U HpaII enzyme). DNA is digested for 3 hours at 37°C, split in 4 aliquots, and diluted in 8 ml ligation buffer (1X ligation buffer NEB without ATP, 1 mM ATP, 0.1 mg/ml BSA, 125 Units of T4 DNA ligase 5 U/μl). Ligation is then performed at 16°C for 4 hours, followed by incubation overnight (ON) at 65°C in presence of 250 μg/ml proteinase K and 5 mM EDTA. Precipitation of DNA is performed using an equal volume of 3 M Na-Acetate (pH 5.2) and two volumes of iso-propanol. After one hour at -80°C, DNA is pelleted, suspended in 500μl 1X TE buffer, and incubated for 30 minutes at 37°C in presence of RNAse A (0.03 mg/ml). DNA is then transferred into 2 ml centrifuge tubes, extracted twice with 500 μl phenol-chloroform pH 8.0, precipitated, washed with 1 ml cold ethanol 70% and diluted in 30 μl 1X TE buffer. All tubes are pooled and the resulting 3C library is quantified on gel using Quantity One software (BioRad). PGCGROWTHCONDITIONS SRR6354579 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cells were grown at 22 °C , 30 °C and <Temp> 37 °C </Temp> in either Lennox Broth ( LB ) or liquid minimal medium A supplemented with 0.12 % casamino acids and 0.4 % glucose . The cultures were grown to OD600 = 0.2 ( early exponential ) or 2 ( stationary phase ) . GPL15010-GPL21117 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL21117: Illumina NextSeq 500 (Escherichia coli str. K-12 substr. MG1655) GSE107301 Multiscale structuring of the E. coli chromosome by nucleoid-associated and condensin proteins GSM2870441 RNA-seq of E. coli wt cells in stationary phase 30°C (30h) -Replicate 1 29358050 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107301/GSE107301.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cells were grown at 22°C, 30°C and 37°C in either Lennox Broth (LB) or liquid minimal medium A supplemented with 0.12% casamino acids and 0.4% glucose. The cultures were grown to OD600 = 0.2 (early exponential) or 2 (stationary phase). PGCGROWTHCONDITIONS SRR6354579 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Separate E. coli colonies were picked from LB agar plates for each replicate . Overnight cultures ( 12-16 hrs ) were grown at <Temp> 37 °C </Temp> in M9 media supplemented with 0.1 % casamino acids , 0.4 % glycerol , 0.4 % glucose , 2 mM MgSO4 , and 0.1 mM CaCl2 . Overnight cultures were back diluted into fresh <Med> media </Med> and grown 3-4 hours to an <OD> OD600 of ~ 0.35 </OD> at <Temp> 37 °C </Temp> in an orbital shaker at 200 RPM . Cultures were centrifuged , washed , and back diluted into media lacking glucose . After at least 30 minutes of recovery Para was induced by adding arabinose to 0.2 % . Cells were harvested by centrifuging 1 mL of culture for 1 minute at maximum speed on a benchtop centrifuge . Pellets were immediately flash frozen in lN2 . GPL21222 GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE107327 E. coli MazF does not create specialized ribosomes that translate leaderless mRNAs, but instead blocks rRNA maturation and ribosome biogenesis [RNA-seq] GSM2864853 emptyvec_mRNA_5m_rep1 29861158 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107327/GSE107327.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Separate E. coli colonies were picked from LB agar plates for each replicate. Overnight cultures (12-16 hrs) were grown at 37°C in M9 media supplemented with 0.1% casamino acids, 0.4% glycerol, 0.4% glucose, 2 mM MgSO4, and 0.1 mM CaCl2. Overnight cultures were back diluted into fresh media and grown 3-4 hours to an OD600 of ~0.35 at 37°C in an orbital shaker at 200 RPM. Cultures were centrifuged, washed, and back diluted into media lacking glucose. After at least 30 minutes of recovery Para was induced by adding arabinose to 0.2%. Cells were harvested by centrifuging 1 mL of culture for 1 minute at maximum speed on a benchtop centrifuge. Pellets were immediately flash frozen in lN2. PGCGROWTHCONDITIONS SRR6322033 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Separate E. coli colonies were picked from LB agar plates for each replicate . Overnight cultures ( 12-16 hrs ) were grown at <Temp> 37 °C </Temp> in M9 media supplemented with 0.1 % casamino acids , 0.4 % glycerol , 0.4 % glucose , 2 mM MgSO4 , and 0.1 mM CaCl2 . Overnight cultures were back diluted into fresh <Med> media </Med> and grown 3-4 hours to an <OD> OD600 of ~ 0.35 </OD> at <Temp> 37 °C </Temp> in an orbital shaker at 200 RPM . Cultures were centrifuged , washed , and back diluted into media lacking glucose . After at least 30 minutes of recovery Para was induced by adding arabinose to 0.2 % . Cells were harvested by centrifuging 1 mL of culture for 1 minute at maximum speed on a benchtop centrifuge . Pellets were immediately flash frozen in lN2 . GPL21222 GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE107327 E. coli MazF does not create specialized ribosomes that translate leaderless mRNAs, but instead blocks rRNA maturation and ribosome biogenesis [RNA-seq] GSM2864854 emptyvec_mRNA_5m_rep2 29861158 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107327/GSE107327.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Separate E. coli colonies were picked from LB agar plates for each replicate. Overnight cultures (12-16 hrs) were grown at 37°C in M9 media supplemented with 0.1% casamino acids, 0.4% glycerol, 0.4% glucose, 2 mM MgSO4, and 0.1 mM CaCl2. Overnight cultures were back diluted into fresh media and grown 3-4 hours to an OD600 of ~0.35 at 37°C in an orbital shaker at 200 RPM. Cultures were centrifuged, washed, and back diluted into media lacking glucose. After at least 30 minutes of recovery Para was induced by adding arabinose to 0.2%. Cells were harvested by centrifuging 1 mL of culture for 1 minute at maximum speed on a benchtop centrifuge. Pellets were immediately flash frozen in lN2. PGCGROWTHCONDITIONS SRR6322034 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Separate E. coli colonies were picked from LB agar plates for each replicate . Overnight cultures ( 12-16 hrs ) were grown at <Temp> 37 °C </Temp> in M9 media supplemented with 0.1 % casamino acids , 0.4 % glycerol , 0.4 % glucose , 2 mM MgSO4 , and 0.1 mM CaCl2 . Overnight cultures were back diluted into fresh <Med> media </Med> and grown 3-4 hours to an <OD> OD600 of ~ 0.35 </OD> at <Temp> 37 °C </Temp> in an orbital shaker at 200 RPM . Cultures were centrifuged , washed , and back diluted into media lacking glucose . After at least 30 minutes of recovery Para was induced by adding arabinose to 0.2 % . Cells were harvested by centrifuging 1 mL of culture for 1 minute at maximum speed on a benchtop centrifuge . Pellets were immediately flash frozen in lN2 . GPL21222 GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE107327 E. coli MazF does not create specialized ribosomes that translate leaderless mRNAs, but instead blocks rRNA maturation and ribosome biogenesis [RNA-seq] GSM2864855 MazF_mRNA_5m_rep1 29861158 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107327/GSE107327.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Separate E. coli colonies were picked from LB agar plates for each replicate. Overnight cultures (12-16 hrs) were grown at 37°C in M9 media supplemented with 0.1% casamino acids, 0.4% glycerol, 0.4% glucose, 2 mM MgSO4, and 0.1 mM CaCl2. Overnight cultures were back diluted into fresh media and grown 3-4 hours to an OD600 of ~0.35 at 37°C in an orbital shaker at 200 RPM. Cultures were centrifuged, washed, and back diluted into media lacking glucose. After at least 30 minutes of recovery Para was induced by adding arabinose to 0.2%. Cells were harvested by centrifuging 1 mL of culture for 1 minute at maximum speed on a benchtop centrifuge. Pellets were immediately flash frozen in lN2. PGCGROWTHCONDITIONS SRR6322035 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Separate E. coli colonies were picked from LB agar plates for each replicate . Overnight cultures ( 12-16 hrs ) were grown at <Temp> 37 °C </Temp> in M9 media supplemented with 0.1 % casamino acids , 0.4 % glycerol , 0.4 % glucose , 2 mM MgSO4 , and 0.1 mM CaCl2 . Overnight cultures were back diluted into fresh <Med> media </Med> and grown 3-4 hours to an <OD> OD600 of ~ 0.35 </OD> at <Temp> 37 °C </Temp> in an orbital shaker at 200 RPM . Cultures were centrifuged , washed , and back diluted into media lacking glucose . After at least 30 minutes of recovery Para was induced by adding arabinose to 0.2 % . Cells were harvested by centrifuging 1 mL of culture for 1 minute at maximum speed on a benchtop centrifuge . Pellets were immediately flash frozen in lN2 . GPL21222 GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE107327 E. coli MazF does not create specialized ribosomes that translate leaderless mRNAs, but instead blocks rRNA maturation and ribosome biogenesis [RNA-seq] GSM2864856 MazF_mRNA_5m_rep2 29861158 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107327/GSE107327.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Separate E. coli colonies were picked from LB agar plates for each replicate. Overnight cultures (12-16 hrs) were grown at 37°C in M9 media supplemented with 0.1% casamino acids, 0.4% glycerol, 0.4% glucose, 2 mM MgSO4, and 0.1 mM CaCl2. Overnight cultures were back diluted into fresh media and grown 3-4 hours to an OD600 of ~0.35 at 37°C in an orbital shaker at 200 RPM. Cultures were centrifuged, washed, and back diluted into media lacking glucose. After at least 30 minutes of recovery Para was induced by adding arabinose to 0.2%. Cells were harvested by centrifuging 1 mL of culture for 1 minute at maximum speed on a benchtop centrifuge. Pellets were immediately flash frozen in lN2. PGCGROWTHCONDITIONS SRR6322036 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C RNA was extracted using Trizol and chloroform in conjunction with Qiagen 's RNeasy Mini kit ( Qiagen , Valencia , CA ) following the A. Untergasser protocol ( http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm ) with one modification : <Gtype> Phase Lock Gel </Gtype> tubes ( Eppendorf , Westbury , NY ) were used to better separate organic and aqueous phases after the addition of chloroform . DNA was digested with Ambion rDNAse I ( Life Technologies , Carlsbad , CA ) for <Supp> 30 min </Supp> at <Temp> 37 °C </Temp> . Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 ( Agilent Technologies , Santa Clara , CA ) . Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit ( Life Technologies , Carlsbad , CA ) and were used for library construction . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914313 CF104.3.3_y2 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt RNA was extracted using Trizol and chloroform in conjunction with Qiagen’s RNeasy Mini kit (Qiagen, Valencia, CA) following the A. Untergasser protocol (http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm) with one modification: Phase Lock Gel tubes (Eppendorf, Westbury, NY) were used to better separate organic and aqueous phases after the addition of chloroform. DNA was digested with Ambion rDNAse I (Life Technologies, Carlsbad, CA) for 30 min at 37°C. Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 (Agilent Technologies, Santa Clara, CA). Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit (Life Technologies, Carlsbad, CA) and were used for library construction. PGCGROWTHCONDITIONS SRR6449107 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914313 CF104.3.3_y2 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449107 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C RNA was extracted using Trizol and chloroform in conjunction with Qiagen 's RNeasy Mini kit ( Qiagen , Valencia , CA ) following the A. Untergasser protocol ( http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm ) with one modification : <Gtype> Phase Lock Gel </Gtype> tubes ( Eppendorf , Westbury , NY ) were used to better separate organic and aqueous phases after the addition of chloroform . DNA was digested with Ambion rDNAse I ( Life Technologies , Carlsbad , CA ) for <Supp> 30 min </Supp> at <Temp> 37 °C </Temp> . Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 ( Agilent Technologies , Santa Clara , CA ) . Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit ( Life Technologies , Carlsbad , CA ) and were used for library construction . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914315 CF108.4B_y3 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt RNA was extracted using Trizol and chloroform in conjunction with Qiagen’s RNeasy Mini kit (Qiagen, Valencia, CA) following the A. Untergasser protocol (http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm) with one modification: Phase Lock Gel tubes (Eppendorf, Westbury, NY) were used to better separate organic and aqueous phases after the addition of chloroform. DNA was digested with Ambion rDNAse I (Life Technologies, Carlsbad, CA) for 30 min at 37°C. Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 (Agilent Technologies, Santa Clara, CA). Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit (Life Technologies, Carlsbad, CA) and were used for library construction. PGCGROWTHCONDITIONS SRR6449109 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914315 CF108.4B_y3 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449109 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C RNA was extracted using Trizol and chloroform in conjunction with Qiagen 's RNeasy Mini kit ( Qiagen , Valencia , CA ) following the A. Untergasser protocol ( http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm ) with one modification : <Gtype> Phase Lock Gel </Gtype> tubes ( Eppendorf , Westbury , NY ) were used to better separate organic and aqueous phases after the addition of chloroform . DNA was digested with Ambion rDNAse I ( Life Technologies , Carlsbad , CA ) for <Supp> 30 min </Supp> at <Temp> 37 °C </Temp> . Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 ( Agilent Technologies , Santa Clara , CA ) . Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit ( Life Technologies , Carlsbad , CA ) and were used for library construction . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914317 CON206.3A_y1 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt RNA was extracted using Trizol and chloroform in conjunction with Qiagen’s RNeasy Mini kit (Qiagen, Valencia, CA) following the A. Untergasser protocol (http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm) with one modification: Phase Lock Gel tubes (Eppendorf, Westbury, NY) were used to better separate organic and aqueous phases after the addition of chloroform. DNA was digested with Ambion rDNAse I (Life Technologies, Carlsbad, CA) for 30 min at 37°C. Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 (Agilent Technologies, Santa Clara, CA). Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit (Life Technologies, Carlsbad, CA) and were used for library construction. PGCGROWTHCONDITIONS SRR6449111 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914317 CON206.3A_y1 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449111 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C RNA was extracted using Trizol and chloroform in conjunction with Qiagen 's RNeasy Mini kit ( Qiagen , Valencia , CA ) following the A. Untergasser protocol ( http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm ) with one modification : <Gtype> Phase Lock Gel </Gtype> tubes ( Eppendorf , Westbury , NY ) were used to better separate organic and aqueous phases after the addition of chloroform . DNA was digested with Ambion rDNAse I ( Life Technologies , Carlsbad , CA ) for <Supp> 30 min </Supp> at <Temp> 37 °C </Temp> . Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 ( Agilent Technologies , Santa Clara , CA ) . Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit ( Life Technologies , Carlsbad , CA ) and were used for library construction . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914318 CON206.3A_y2 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt RNA was extracted using Trizol and chloroform in conjunction with Qiagen’s RNeasy Mini kit (Qiagen, Valencia, CA) following the A. Untergasser protocol (http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm) with one modification: Phase Lock Gel tubes (Eppendorf, Westbury, NY) were used to better separate organic and aqueous phases after the addition of chloroform. DNA was digested with Ambion rDNAse I (Life Technologies, Carlsbad, CA) for 30 min at 37°C. Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 (Agilent Technologies, Santa Clara, CA). Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit (Life Technologies, Carlsbad, CA) and were used for library construction. PGCGROWTHCONDITIONS SRR6449112 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914318 CON206.3A_y2 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449112 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C RNA was extracted using Trizol and chloroform in conjunction with Qiagen 's RNeasy Mini kit ( Qiagen , Valencia , CA ) following the A. Untergasser protocol ( http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm ) with one modification : <Gtype> Phase Lock Gel </Gtype> tubes ( Eppendorf , Westbury , NY ) were used to better separate organic and aqueous phases after the addition of chloroform . DNA was digested with Ambion rDNAse I ( Life Technologies , Carlsbad , CA ) for <Supp> 30 min </Supp> at <Temp> 37 °C </Temp> . Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 ( Agilent Technologies , Santa Clara , CA ) . Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit ( Life Technologies , Carlsbad , CA ) and were used for library construction . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914319 CON208.3A_y2 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt RNA was extracted using Trizol and chloroform in conjunction with Qiagen’s RNeasy Mini kit (Qiagen, Valencia, CA) following the A. Untergasser protocol (http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm) with one modification: Phase Lock Gel tubes (Eppendorf, Westbury, NY) were used to better separate organic and aqueous phases after the addition of chloroform. DNA was digested with Ambion rDNAse I (Life Technologies, Carlsbad, CA) for 30 min at 37°C. Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 (Agilent Technologies, Santa Clara, CA). Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit (Life Technologies, Carlsbad, CA) and were used for library construction. PGCGROWTHCONDITIONS SRR6449113 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914319 CON208.3A_y2 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449113 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C RNA was extracted using Trizol and chloroform in conjunction with Qiagen 's RNeasy Mini kit ( Qiagen , Valencia , CA ) following the A. Untergasser protocol ( http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm ) with one modification : <Gtype> Phase Lock Gel </Gtype> tubes ( Eppendorf , Westbury , NY ) were used to better separate organic and aqueous phases after the addition of chloroform . DNA was digested with Ambion rDNAse I ( Life Technologies , Carlsbad , CA ) for <Supp> 30 min </Supp> at <Temp> 37 °C </Temp> . Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 ( Agilent Technologies , Santa Clara , CA ) . Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit ( Life Technologies , Carlsbad , CA ) and were used for library construction . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914320 CON208.3A_y6 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt RNA was extracted using Trizol and chloroform in conjunction with Qiagen’s RNeasy Mini kit (Qiagen, Valencia, CA) following the A. Untergasser protocol (http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm) with one modification: Phase Lock Gel tubes (Eppendorf, Westbury, NY) were used to better separate organic and aqueous phases after the addition of chloroform. DNA was digested with Ambion rDNAse I (Life Technologies, Carlsbad, CA) for 30 min at 37°C. Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 (Agilent Technologies, Santa Clara, CA). Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit (Life Technologies, Carlsbad, CA) and were used for library construction. PGCGROWTHCONDITIONS SRR6449114 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914320 CON208.3A_y6 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449114 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C RNA was extracted using Trizol and chloroform in conjunction with Qiagen 's RNeasy Mini kit ( Qiagen , Valencia , CA ) following the A. Untergasser protocol ( http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm ) with one modification : <Gtype> Phase Lock Gel </Gtype> tubes ( Eppendorf , Westbury , NY ) were used to better separate organic and aqueous phases after the addition of chloroform . DNA was digested with Ambion rDNAse I ( Life Technologies , Carlsbad , CA ) for <Supp> 30 min </Supp> at <Temp> 37 °C </Temp> . Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 ( Agilent Technologies , Santa Clara , CA ) . Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit ( Life Technologies , Carlsbad , CA ) and were used for library construction . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914321 CF104.3.3_u1 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt RNA was extracted using Trizol and chloroform in conjunction with Qiagen’s RNeasy Mini kit (Qiagen, Valencia, CA) following the A. Untergasser protocol (http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm) with one modification: Phase Lock Gel tubes (Eppendorf, Westbury, NY) were used to better separate organic and aqueous phases after the addition of chloroform. DNA was digested with Ambion rDNAse I (Life Technologies, Carlsbad, CA) for 30 min at 37°C. Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 (Agilent Technologies, Santa Clara, CA). Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit (Life Technologies, Carlsbad, CA) and were used for library construction. PGCGROWTHCONDITIONS SRR6449115 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914321 CF104.3.3_u1 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449115 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C RNA was extracted using Trizol and chloroform in conjunction with Qiagen 's RNeasy Mini kit ( Qiagen , Valencia , CA ) following the A. Untergasser protocol ( http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm ) with one modification : <Gtype> Phase Lock Gel </Gtype> tubes ( Eppendorf , Westbury , NY ) were used to better separate organic and aqueous phases after the addition of chloroform . DNA was digested with Ambion rDNAse I ( Life Technologies , Carlsbad , CA ) for <Supp> 30 min </Supp> at <Temp> 37 °C </Temp> . Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 ( Agilent Technologies , Santa Clara , CA ) . Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit ( Life Technologies , Carlsbad , CA ) and were used for library construction . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914322 CF104.3.3_u7 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt RNA was extracted using Trizol and chloroform in conjunction with Qiagen’s RNeasy Mini kit (Qiagen, Valencia, CA) following the A. Untergasser protocol (http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm) with one modification: Phase Lock Gel tubes (Eppendorf, Westbury, NY) were used to better separate organic and aqueous phases after the addition of chloroform. DNA was digested with Ambion rDNAse I (Life Technologies, Carlsbad, CA) for 30 min at 37°C. Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 (Agilent Technologies, Santa Clara, CA). Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit (Life Technologies, Carlsbad, CA) and were used for library construction. PGCGROWTHCONDITIONS SRR6449116 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914322 CF104.3.3_u7 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449116 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C RNA was extracted using Trizol and chloroform in conjunction with Qiagen 's RNeasy Mini kit ( Qiagen , Valencia , CA ) following the A. Untergasser protocol ( http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm ) with one modification : <Gtype> Phase Lock Gel </Gtype> tubes ( Eppendorf , Westbury , NY ) were used to better separate organic and aqueous phases after the addition of chloroform . DNA was digested with Ambion rDNAse I ( Life Technologies , Carlsbad , CA ) for <Supp> 30 min </Supp> at <Temp> 37 °C </Temp> . Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 ( Agilent Technologies , Santa Clara , CA ) . Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit ( Life Technologies , Carlsbad , CA ) and were used for library construction . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914323 CF108.4B_u1 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt RNA was extracted using Trizol and chloroform in conjunction with Qiagen’s RNeasy Mini kit (Qiagen, Valencia, CA) following the A. Untergasser protocol (http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm) with one modification: Phase Lock Gel tubes (Eppendorf, Westbury, NY) were used to better separate organic and aqueous phases after the addition of chloroform. DNA was digested with Ambion rDNAse I (Life Technologies, Carlsbad, CA) for 30 min at 37°C. Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 (Agilent Technologies, Santa Clara, CA). Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit (Life Technologies, Carlsbad, CA) and were used for library construction. PGCGROWTHCONDITIONS SRR6449117 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914323 CF108.4B_u1 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449117 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C RNA was extracted using Trizol and chloroform in conjunction with Qiagen 's RNeasy Mini kit ( Qiagen , Valencia , CA ) following the A. Untergasser protocol ( http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm ) with one modification : <Gtype> Phase Lock Gel </Gtype> tubes ( Eppendorf , Westbury , NY ) were used to better separate organic and aqueous phases after the addition of chloroform . DNA was digested with Ambion rDNAse I ( Life Technologies , Carlsbad , CA ) for <Supp> 30 min </Supp> at <Temp> 37 °C </Temp> . Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 ( Agilent Technologies , Santa Clara , CA ) . Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit ( Life Technologies , Carlsbad , CA ) and were used for library construction . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914324 CF108.4B_u2 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt RNA was extracted using Trizol and chloroform in conjunction with Qiagen’s RNeasy Mini kit (Qiagen, Valencia, CA) following the A. Untergasser protocol (http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm) with one modification: Phase Lock Gel tubes (Eppendorf, Westbury, NY) were used to better separate organic and aqueous phases after the addition of chloroform. DNA was digested with Ambion rDNAse I (Life Technologies, Carlsbad, CA) for 30 min at 37°C. Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 (Agilent Technologies, Santa Clara, CA). Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit (Life Technologies, Carlsbad, CA) and were used for library construction. PGCGROWTHCONDITIONS SRR6449118 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914324 CF108.4B_u2 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449118 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C RNA was extracted using Trizol and chloroform in conjunction with Qiagen 's RNeasy Mini kit ( Qiagen , Valencia , CA ) following the A. Untergasser protocol ( http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm ) with one modification : <Gtype> Phase Lock Gel </Gtype> tubes ( Eppendorf , Westbury , NY ) were used to better separate organic and aqueous phases after the addition of chloroform . DNA was digested with Ambion rDNAse I ( Life Technologies , Carlsbad , CA ) for <Supp> 30 min </Supp> at <Temp> 37 °C </Temp> . Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 ( Agilent Technologies , Santa Clara , CA ) . Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit ( Life Technologies , Carlsbad , CA ) and were used for library construction . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914325 CON206.3A_u1 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt RNA was extracted using Trizol and chloroform in conjunction with Qiagen’s RNeasy Mini kit (Qiagen, Valencia, CA) following the A. Untergasser protocol (http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm) with one modification: Phase Lock Gel tubes (Eppendorf, Westbury, NY) were used to better separate organic and aqueous phases after the addition of chloroform. DNA was digested with Ambion rDNAse I (Life Technologies, Carlsbad, CA) for 30 min at 37°C. Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 (Agilent Technologies, Santa Clara, CA). Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit (Life Technologies, Carlsbad, CA) and were used for library construction. PGCGROWTHCONDITIONS SRR6449119 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914325 CON206.3A_u1 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449119 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C RNA was extracted using Trizol and chloroform in conjunction with Qiagen 's RNeasy Mini kit ( Qiagen , Valencia , CA ) following the A. Untergasser protocol ( http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm ) with one modification : <Gtype> Phase Lock Gel </Gtype> tubes ( Eppendorf , Westbury , NY ) were used to better separate organic and aqueous phases after the addition of chloroform . DNA was digested with Ambion rDNAse I ( Life Technologies , Carlsbad , CA ) for <Supp> 30 min </Supp> at <Temp> 37 °C </Temp> . Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 ( Agilent Technologies , Santa Clara , CA ) . Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit ( Life Technologies , Carlsbad , CA ) and were used for library construction . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914326 CON206.3A_u2 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt RNA was extracted using Trizol and chloroform in conjunction with Qiagen’s RNeasy Mini kit (Qiagen, Valencia, CA) following the A. Untergasser protocol (http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm) with one modification: Phase Lock Gel tubes (Eppendorf, Westbury, NY) were used to better separate organic and aqueous phases after the addition of chloroform. DNA was digested with Ambion rDNAse I (Life Technologies, Carlsbad, CA) for 30 min at 37°C. Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 (Agilent Technologies, Santa Clara, CA). Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit (Life Technologies, Carlsbad, CA) and were used for library construction. PGCGROWTHCONDITIONS SRR6449120 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914326 CON206.3A_u2 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449120 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C RNA was extracted using Trizol and chloroform in conjunction with Qiagen 's RNeasy Mini kit ( Qiagen , Valencia , CA ) following the A. Untergasser protocol ( http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm ) with one modification : <Gtype> Phase Lock Gel </Gtype> tubes ( Eppendorf , Westbury , NY ) were used to better separate organic and aqueous phases after the addition of chloroform . DNA was digested with Ambion rDNAse I ( Life Technologies , Carlsbad , CA ) for <Supp> 30 min </Supp> at <Temp> 37 °C </Temp> . Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 ( Agilent Technologies , Santa Clara , CA ) . Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit ( Life Technologies , Carlsbad , CA ) and were used for library construction . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914327 CON208.3A_u1 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt RNA was extracted using Trizol and chloroform in conjunction with Qiagen’s RNeasy Mini kit (Qiagen, Valencia, CA) following the A. Untergasser protocol (http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm) with one modification: Phase Lock Gel tubes (Eppendorf, Westbury, NY) were used to better separate organic and aqueous phases after the addition of chloroform. DNA was digested with Ambion rDNAse I (Life Technologies, Carlsbad, CA) for 30 min at 37°C. Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 (Agilent Technologies, Santa Clara, CA). Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit (Life Technologies, Carlsbad, CA) and were used for library construction. PGCGROWTHCONDITIONS SRR6449121 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914327 CON208.3A_u1 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449121 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C RNA was extracted using Trizol and chloroform in conjunction with Qiagen 's RNeasy Mini kit ( Qiagen , Valencia , CA ) following the A. Untergasser protocol ( http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm ) with one modification : <Gtype> Phase Lock Gel </Gtype> tubes ( Eppendorf , Westbury , NY ) were used to better separate organic and aqueous phases after the addition of chloroform . DNA was digested with Ambion rDNAse I ( Life Technologies , Carlsbad , CA ) for <Supp> 30 min </Supp> at <Temp> 37 °C </Temp> . Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 ( Agilent Technologies , Santa Clara , CA ) . Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit ( Life Technologies , Carlsbad , CA ) and were used for library construction . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914328 CON208.3A_u8 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt RNA was extracted using Trizol and chloroform in conjunction with Qiagen’s RNeasy Mini kit (Qiagen, Valencia, CA) following the A. Untergasser protocol (http://www.molbi.de/protocols/rna_prep_comb_trizol_v1_0.htm) with one modification: Phase Lock Gel tubes (Eppendorf, Westbury, NY) were used to better separate organic and aqueous phases after the addition of chloroform. DNA was digested with Ambion rDNAse I (Life Technologies, Carlsbad, CA) for 30 min at 37°C. Total RNA was further cleaned using the RNeasy Mini kit and quality was assessed by the RNA ScreenTape Assay on the TapeStation 2200 (Agilent Technologies, Santa Clara, CA). Samples with RIN values greater than 8.0 were treated with the Ambion MICROBExpress Bacterial mRNA Enrichment Kit (Life Technologies, Carlsbad, CA) and were used for library construction. PGCGROWTHCONDITIONS SRR6449122 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914328 CON208.3A_u8 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449122 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG 1655 was grown in LB-Miller broth at <Temp> 37 °C </Temp> ,160 rpm to an O.D. 600 nm of 0 . 7 GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE114917 Genetic response of E.coli to mild elevated pressure GSM3154484 HP1 30016375 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE114917/GSE114917.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG 1655 was grown in LB-Miller broth at 37°C ,160rpm to an O.D.600 nm of 0. 7 PGCGROWTHCONDITIONS SRR7217923 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG 1655 was grown in LB-Miller broth at <Temp> 37 °C </Temp> ,160 rpm to an O.D. 600 nm of 0 . 7 GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE114917 Genetic response of E.coli to mild elevated pressure GSM3154485 HP2 30016375 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE114917/GSE114917.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG 1655 was grown in LB-Miller broth at 37°C ,160rpm to an O.D.600 nm of 0. 7 PGCGROWTHCONDITIONS SRR7217924 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG 1655 was grown in LB-Miller broth at <Temp> 37 °C </Temp> ,160 rpm to an O.D. 600 nm of 0 . 7 GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE114917 Genetic response of E.coli to mild elevated pressure GSM3154486 HP3 30016375 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE114917/GSE114917.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG 1655 was grown in LB-Miller broth at 37°C ,160rpm to an O.D.600 nm of 0. 7 PGCGROWTHCONDITIONS SRR7217925 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG 1655 was grown in LB-Miller broth at <Temp> 37 °C </Temp> ,160 rpm to an O.D. 600 nm of 0 . 7 GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE114917 Genetic response of E.coli to mild elevated pressure GSM3154487 HP4 30016375 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE114917/GSE114917.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG 1655 was grown in LB-Miller broth at 37°C ,160rpm to an O.D.600 nm of 0. 7 PGCGROWTHCONDITIONS SRR7217926 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG 1655 was grown in LB-Miller broth at <Temp> 37 °C </Temp> ,160 rpm to an O.D. 600 nm of 0 . 7 GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE114917 Genetic response of E.coli to mild elevated pressure GSM3154488 LP1 30016375 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE114917/GSE114917.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG 1655 was grown in LB-Miller broth at 37°C ,160rpm to an O.D.600 nm of 0. 7 PGCGROWTHCONDITIONS SRR7217927 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG 1655 was grown in LB-Miller broth at <Temp> 37 °C </Temp> ,160 rpm to an O.D. 600 nm of 0 . 7 GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE114917 Genetic response of E.coli to mild elevated pressure GSM3154489 LP2 30016375 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE114917/GSE114917.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG 1655 was grown in LB-Miller broth at 37°C ,160rpm to an O.D.600 nm of 0. 7 PGCGROWTHCONDITIONS SRR7217928 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG 1655 was grown in LB-Miller broth at <Temp> 37 °C </Temp> ,160 rpm to an O.D. 600 nm of 0 . 7 GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE114917 Genetic response of E.coli to mild elevated pressure GSM3154490 LP3 30016375 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE114917/GSE114917.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG 1655 was grown in LB-Miller broth at 37°C ,160rpm to an O.D.600 nm of 0. 7 PGCGROWTHCONDITIONS SRR7217929 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG 1655 was grown in LB-Miller broth at <Temp> 37 °C </Temp> ,160 rpm to an O.D. 600 nm of 0 . 7 GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE114917 Genetic response of E.coli to mild elevated pressure GSM3154491 LP4 30016375 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE114917/GSE114917.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG 1655 was grown in LB-Miller broth at 37°C ,160rpm to an O.D.600 nm of 0. 7 PGCGROWTHCONDITIONS SRR7217930 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Plasmid pLCenvZ or pLCenvZM was co-transformed with pPCB into the competent cells of E. coli JW3367 and spread on LB agar plates containing Ampicillin ( 50 ng/μl ) and Chloramphenicol ( 170 ng/μl ) at <Temp> 37 °C </Temp> overnight . A single colony was diluted in 2.5 ml LB medium containing Ampicillin ( 50 ng/μl ) and Chloramphenicol ( 170 ng/μl ) and was shaken at 250 rpm and <Temp> 37 °C </Temp> overnight . GPL25346 GPL25346: HiSeq X Ten (Escherichia coli) GSE117326 Gene expression profiles in E. coli under different frequency signals GSM3291044 envz600 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117326/GSE117326.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Plasmid pLCenvZ or pLCenvZM was co-transformed with pPCB into the competent cells of E. coli JW3367 and spread on LB agar plates containing Ampicillin (50 ng/μl) and Chloramphenicol (170 ng/μl) at 37°C overnight. A single colony was diluted in 2.5ml LB medium containing Ampicillin (50 ng/μl) and Chloramphenicol (170 ng/μl) and was shaken at 250 rpm and 37°C overnight. PGCGROWTHCONDITIONS SRR7537397 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Plasmid pLCenvZ or pLCenvZM was co-transformed with pPCB into the competent cells of E. coli JW3367 and spread on LB agar plates containing Ampicillin ( 50 ng/μl ) and Chloramphenicol ( 170 ng/μl ) at <Temp> 37 °C </Temp> overnight . A single colony was diluted in 2.5 ml LB medium containing Ampicillin ( 50 ng/μl ) and Chloramphenicol ( 170 ng/μl ) and was shaken at 250 rpm and <Temp> 37 °C </Temp> overnight . GPL25346 GPL25346: HiSeq X Ten (Escherichia coli) GSE117326 Gene expression profiles in E. coli under different frequency signals GSM3291045 envz900 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117326/GSE117326.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Plasmid pLCenvZ or pLCenvZM was co-transformed with pPCB into the competent cells of E. coli JW3367 and spread on LB agar plates containing Ampicillin (50 ng/μl) and Chloramphenicol (170 ng/μl) at 37°C overnight. A single colony was diluted in 2.5ml LB medium containing Ampicillin (50 ng/μl) and Chloramphenicol (170 ng/μl) and was shaken at 250 rpm and 37°C overnight. PGCGROWTHCONDITIONS SRR7537398 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Plasmid pLCenvZ or pLCenvZM was co-transformed with pPCB into the competent cells of E. coli JW3367 and spread on LB agar plates containing Ampicillin ( 50 ng/μl ) and Chloramphenicol ( 170 ng/μl ) at <Temp> 37 °C </Temp> overnight . A single colony was diluted in 2.5 ml LB medium containing Ampicillin ( 50 ng/μl ) and Chloramphenicol ( 170 ng/μl ) and was shaken at 250 rpm and <Temp> 37 °C </Temp> overnight . GPL25346 GPL25346: HiSeq X Ten (Escherichia coli) GSE117326 Gene expression profiles in E. coli under different frequency signals GSM3291049 envz3600 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117326/GSE117326.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Plasmid pLCenvZ or pLCenvZM was co-transformed with pPCB into the competent cells of E. coli JW3367 and spread on LB agar plates containing Ampicillin (50 ng/μl) and Chloramphenicol (170 ng/μl) at 37°C overnight. A single colony was diluted in 2.5ml LB medium containing Ampicillin (50 ng/μl) and Chloramphenicol (170 ng/μl) and was shaken at 250 rpm and 37°C overnight. PGCGROWTHCONDITIONS SRR7537402 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Plasmid pLCenvZ or pLCenvZM was co-transformed with pPCB into the competent cells of E. coli JW3367 and spread on LB agar plates containing Ampicillin ( 50 ng/μl ) and Chloramphenicol ( 170 ng/μl ) at <Temp> 37 °C </Temp> overnight . A single colony was diluted in 2.5 ml LB medium containing Ampicillin ( 50 ng/μl ) and Chloramphenicol ( 170 ng/μl ) and was shaken at 250 rpm and <Temp> 37 °C </Temp> overnight . GPL25346 GPL25346: HiSeq X Ten (Escherichia coli) GSE117326 Gene expression profiles in E. coli under different frequency signals GSM3291050 envzM600 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117326/GSE117326.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Plasmid pLCenvZ or pLCenvZM was co-transformed with pPCB into the competent cells of E. coli JW3367 and spread on LB agar plates containing Ampicillin (50 ng/μl) and Chloramphenicol (170 ng/μl) at 37°C overnight. A single colony was diluted in 2.5ml LB medium containing Ampicillin (50 ng/μl) and Chloramphenicol (170 ng/μl) and was shaken at 250 rpm and 37°C overnight. PGCGROWTHCONDITIONS SRR7537403 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Plasmid pLCenvZ or pLCenvZM was co-transformed with pPCB into the competent cells of E. coli JW3367 and spread on LB agar plates containing Ampicillin ( 50 ng/μl ) and Chloramphenicol ( 170 ng/μl ) at <Temp> 37 °C </Temp> overnight . A single colony was diluted in 2.5 ml LB medium containing Ampicillin ( 50 ng/μl ) and Chloramphenicol ( 170 ng/μl ) and was shaken at 250 rpm and <Temp> 37 °C </Temp> overnight . GPL25346 GPL25346: HiSeq X Ten (Escherichia coli) GSE117326 Gene expression profiles in E. coli under different frequency signals GSM3291051 envzM900 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117326/GSE117326.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Plasmid pLCenvZ or pLCenvZM was co-transformed with pPCB into the competent cells of E. coli JW3367 and spread on LB agar plates containing Ampicillin (50 ng/μl) and Chloramphenicol (170 ng/μl) at 37°C overnight. A single colony was diluted in 2.5ml LB medium containing Ampicillin (50 ng/μl) and Chloramphenicol (170 ng/μl) and was shaken at 250 rpm and 37°C overnight. PGCGROWTHCONDITIONS SRR7537404 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Plasmid pLCenvZ or pLCenvZM was co-transformed with pPCB into the competent cells of E. coli JW3367 and spread on LB agar plates containing Ampicillin ( 50 ng/μl ) and Chloramphenicol ( 170 ng/μl ) at <Temp> 37 °C </Temp> overnight . A single colony was diluted in 2.5 ml LB medium containing Ampicillin ( 50 ng/μl ) and Chloramphenicol ( 170 ng/μl ) and was shaken at 250 rpm and <Temp> 37 °C </Temp> overnight . GPL25346 GPL25346: HiSeq X Ten (Escherichia coli) GSE117326 Gene expression profiles in E. coli under different frequency signals GSM3291052 envzM1200 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117326/GSE117326.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Plasmid pLCenvZ or pLCenvZM was co-transformed with pPCB into the competent cells of E. coli JW3367 and spread on LB agar plates containing Ampicillin (50 ng/μl) and Chloramphenicol (170 ng/μl) at 37°C overnight. A single colony was diluted in 2.5ml LB medium containing Ampicillin (50 ng/μl) and Chloramphenicol (170 ng/μl) and was shaken at 250 rpm and 37°C overnight. PGCGROWTHCONDITIONS SRR7537405 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Plasmid pLCenvZ or pLCenvZM was co-transformed with pPCB into the competent cells of E. coli JW3367 and spread on LB agar plates containing Ampicillin ( 50 ng/μl ) and Chloramphenicol ( 170 ng/μl ) at <Temp> 37 °C </Temp> overnight . A single colony was diluted in 2.5 ml LB medium containing Ampicillin ( 50 ng/μl ) and Chloramphenicol ( 170 ng/μl ) and was shaken at 250 rpm and <Temp> 37 °C </Temp> overnight . GPL25346 GPL25346: HiSeq X Ten (Escherichia coli) GSE117326 Gene expression profiles in E. coli under different frequency signals GSM3291054 envzM2400 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117326/GSE117326.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Plasmid pLCenvZ or pLCenvZM was co-transformed with pPCB into the competent cells of E. coli JW3367 and spread on LB agar plates containing Ampicillin (50 ng/μl) and Chloramphenicol (170 ng/μl) at 37°C overnight. A single colony was diluted in 2.5ml LB medium containing Ampicillin (50 ng/μl) and Chloramphenicol (170 ng/μl) and was shaken at 250 rpm and 37°C overnight. PGCGROWTHCONDITIONS SRR7537407 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Plasmid pLCenvZ or pLCenvZM was co-transformed with pPCB into the competent cells of E. coli JW3367 and spread on LB agar plates containing Ampicillin ( 50 ng/μl ) and Chloramphenicol ( 170 ng/μl ) at <Temp> 37 °C </Temp> overnight . A single colony was diluted in 2.5 ml LB medium containing Ampicillin ( 50 ng/μl ) and Chloramphenicol ( 170 ng/μl ) and was shaken at 250 rpm and <Temp> 37 °C </Temp> overnight . GPL25346 GPL25346: HiSeq X Ten (Escherichia coli) GSE117326 Gene expression profiles in E. coli under different frequency signals GSM3291055 envzM3600 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117326/GSE117326.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Plasmid pLCenvZ or pLCenvZM was co-transformed with pPCB into the competent cells of E. coli JW3367 and spread on LB agar plates containing Ampicillin (50 ng/μl) and Chloramphenicol (170 ng/μl) at 37°C overnight. A single colony was diluted in 2.5ml LB medium containing Ampicillin (50 ng/μl) and Chloramphenicol (170 ng/μl) and was shaken at 250 rpm and 37°C overnight. PGCGROWTHCONDITIONS SRR7537408 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C For the RNA-seq samples , the E. coli cells were quenched by adding 0.5 volume of cold Stop Buffer ( 5 % phenol in ethanol ) immediately to the culture medium before harvest and stay on the ice for 15 minutes . Cell pellets were collected by centrifugation ( 6,000 rpm , 5 minutes at 4 °C ) , thoroughly resuspended in 100 μl of lysozyme solution [ 2 mg/ml in TE buffer ( 10 mM Tris-HCl and 1 mM EDTA ) ] , and incubated for 2 minutes . The cells were immediately lysed by adding 1 ml of TRIzol Reagent ( Invitrogen , 15596 ) and the RNA was extracted following manufacture 's instruction . For the ChIP-seq samples , the E. coli cells were fixed with 1 % formaldehyde with continued shaking at <Temp> 37 °C </Temp> for 10 minutes before quenching with glycine ( 100 mM final concentration ) . Cells were then lysed and DNA was sheared by sonication followed by the treatment with micrococcal nuclease and RNase A. Antibody against RNAP β was used for target DNA enrichment . GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566393 RNA-seq WT log phase related to total SEnd-seq WT log phase rep 4 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the RNA-seq samples, the E. coli cells were quenched by adding 0.5 volume of cold Stop Buffer (5% phenol in ethanol) immediately to the culture medium before harvest and stay on the ice for 15 minutes. Cell pellets were collected by centrifugation (6,000 rpm, 5 minutes at 4 °C), thoroughly resuspended in 100 μl of lysozyme solution [2 mg/ml in TE buffer (10 mM Tris-HCl and 1 mM EDTA)], and incubated for 2 minutes. The cells were immediately lysed by adding 1 ml of TRIzol Reagent (Invitrogen, 15596) and the RNA was extracted following manufacture’s instruction. For the ChIP-seq samples, the E. coli cells were fixed with 1% formaldehyde with continued shaking at 37 °C for 10 minutes before quenching with glycine (100 mM final concentration). Cells were then lysed and DNA was sheared by sonication followed by the treatment with micrococcal nuclease and RNase A. Antibody against RNAP β was used for target DNA enrichment. PGCGROWTHCONDITIONS SRR8449235 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Escherichia coli K-12 MG1655 were cultured in <Med> LB media </Med> ( 10 g/L tryptone , 5 g/L yeast extract , 10 g/L NaCl , pH 7.4 ) under aerobic conditions at <Temp> 37 °C </Temp> . The temperature-sensitive RNase E mutant E.coli strain [ rne-3071 ( ts ) ] was cultured in <Med> LB media </Med> at 28 °C for normal culture and shift to 44 °C to deactive RNase E activity . GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566393 RNA-seq WT log phase related to total SEnd-seq WT log phase rep 4 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Escherichia coli K-12 MG1655 were cultured in LB media (10 g/L tryptone, 5 g/L yeast extract, 10 g/L NaCl, pH 7.4) under aerobic conditions at 37 °C. The temperature-sensitive RNase E mutant E.coli strain [rne-3071(ts)] was cultured in LB media at 28 °C for normal culture and shift to 44 °C to deactive RNase E activity. PGCGROWTHCONDITIONS SRR8449235 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C <Gtype> Wild type </Gtype> E. coli , pnp knoctout , rnb knoctout and rnr knoct out strains were cultured in <Med> LB media </Med> at <Temp> 37 °C </Temp> , RNA samples and ChIP-seq samples were collected when the growth stage of indicated cells reached to log phase or stationary phase . rne-3071 ( ts ) mutation strain was cultured in <Med> LB media </Med> at 28 °C to retain the RNase E activity or shifted to 44 °C in water bath for 30 minutes to deactivate RNase E . In order to <Gtype> inactive the rho activity , the wild type ( WT ) Wild type </Gtype> E. coli was cultured in <Med> LB media </Med> with 50 μg/ml bicyclomycin at <Temp> 37 °C </Temp> for 15 min at indicated growth condition . GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566393 RNA-seq WT log phase related to total SEnd-seq WT log phase rep 4 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Wild type E. coli, pnp knoctout, rnb knoctout and rnr knoct out strains were cultured in LB media at 37 °C, RNA samples and ChIP-seq samples were collected when the growth stage of indicated cells reached to log phase or stationary phase. rne-3071(ts) mutation strain was cultured in LB media at 28 °C to retain the RNase E activity or shifted to 44 °C in water bath for 30 minutes to deactivate RNase E. In order to inactive the rho activity, the wild type (WT) Wild type E. coli was cultured in LB media with 50 μg/ml bicyclomycin at 37 °C for 15 min at indicated growth condition. PGCGROWTHCONDITIONS SRR8449235 MCO000003199 37 °C Temperature True
characteristics 35.7 C treatment : <Temp> 37 °C </Temp> culture GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566393 RNA-seq WT log phase related to total SEnd-seq WT log phase rep 4 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 37°C culture PGCGROWTHCONDITIONS SRR8449235 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C For the RNA-seq samples , the E. coli cells were quenched by adding 0.5 volume of cold Stop Buffer ( 5 % phenol in ethanol ) immediately to the culture medium before harvest and stay on the ice for 15 minutes . Cell pellets were collected by centrifugation ( 6,000 rpm , 5 minutes at 4 °C ) , thoroughly resuspended in 100 μl of lysozyme solution [ 2 mg/ml in TE buffer ( 10 mM Tris-HCl and 1 mM EDTA ) ] , and incubated for 2 minutes . The cells were immediately lysed by adding 1 ml of TRIzol Reagent ( Invitrogen , 15596 ) and the RNA was extracted following manufacture 's instruction . For the ChIP-seq samples , the E. coli cells were fixed with 1 % formaldehyde with continued shaking at <Temp> 37 °C </Temp> for 10 minutes before quenching with glycine ( 100 mM final concentration ) . Cells were then lysed and DNA was sheared by sonication followed by the treatment with micrococcal nuclease and RNase A. Antibody against RNAP β was used for target DNA enrichment . GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566394 RNA-seq WT log phase related to total SEnd-seq WT log phase rep 5 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the RNA-seq samples, the E. coli cells were quenched by adding 0.5 volume of cold Stop Buffer (5% phenol in ethanol) immediately to the culture medium before harvest and stay on the ice for 15 minutes. Cell pellets were collected by centrifugation (6,000 rpm, 5 minutes at 4 °C), thoroughly resuspended in 100 μl of lysozyme solution [2 mg/ml in TE buffer (10 mM Tris-HCl and 1 mM EDTA)], and incubated for 2 minutes. The cells were immediately lysed by adding 1 ml of TRIzol Reagent (Invitrogen, 15596) and the RNA was extracted following manufacture’s instruction. For the ChIP-seq samples, the E. coli cells were fixed with 1% formaldehyde with continued shaking at 37 °C for 10 minutes before quenching with glycine (100 mM final concentration). Cells were then lysed and DNA was sheared by sonication followed by the treatment with micrococcal nuclease and RNase A. Antibody against RNAP β was used for target DNA enrichment. PGCGROWTHCONDITIONS SRR8449236 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Escherichia coli K-12 MG1655 were cultured in <Med> LB media </Med> ( 10 g/L tryptone , 5 g/L yeast extract , 10 g/L NaCl , pH 7.4 ) under aerobic conditions at <Temp> 37 °C </Temp> . The temperature-sensitive RNase E mutant E.coli strain [ rne-3071 ( ts ) ] was cultured in <Med> LB media </Med> at 28 °C for normal culture and shift to 44 °C to deactive RNase E activity . GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566394 RNA-seq WT log phase related to total SEnd-seq WT log phase rep 5 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Escherichia coli K-12 MG1655 were cultured in LB media (10 g/L tryptone, 5 g/L yeast extract, 10 g/L NaCl, pH 7.4) under aerobic conditions at 37 °C. The temperature-sensitive RNase E mutant E.coli strain [rne-3071(ts)] was cultured in LB media at 28 °C for normal culture and shift to 44 °C to deactive RNase E activity. PGCGROWTHCONDITIONS SRR8449236 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C <Gtype> Wild type </Gtype> E. coli , pnp knoctout , rnb knoctout and rnr knoct out strains were cultured in <Med> LB media </Med> at <Temp> 37 °C </Temp> , RNA samples and ChIP-seq samples were collected when the growth stage of indicated cells reached to log phase or stationary phase . rne-3071 ( ts ) mutation strain was cultured in <Med> LB media </Med> at 28 °C to retain the RNase E activity or shifted to 44 °C in water bath for 30 minutes to deactivate RNase E . In order to <Gtype> inactive the rho activity , the wild type ( WT ) Wild type </Gtype> E. coli was cultured in <Med> LB media </Med> with 50 μg/ml bicyclomycin at <Temp> 37 °C </Temp> for 15 min at indicated growth condition . GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566394 RNA-seq WT log phase related to total SEnd-seq WT log phase rep 5 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Wild type E. coli, pnp knoctout, rnb knoctout and rnr knoct out strains were cultured in LB media at 37 °C, RNA samples and ChIP-seq samples were collected when the growth stage of indicated cells reached to log phase or stationary phase. rne-3071(ts) mutation strain was cultured in LB media at 28 °C to retain the RNase E activity or shifted to 44 °C in water bath for 30 minutes to deactivate RNase E. In order to inactive the rho activity, the wild type (WT) Wild type E. coli was cultured in LB media with 50 μg/ml bicyclomycin at 37 °C for 15 min at indicated growth condition. PGCGROWTHCONDITIONS SRR8449236 MCO000003199 37 °C Temperature True
characteristics 35.7 C treatment : <Temp> 37 °C </Temp> culture GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566394 RNA-seq WT log phase related to total SEnd-seq WT log phase rep 5 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 37°C culture PGCGROWTHCONDITIONS SRR8449236 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C For the RNA-seq samples , the E. coli cells were quenched by adding 0.5 volume of cold Stop Buffer ( 5 % phenol in ethanol ) immediately to the culture medium before harvest and stay on the ice for 15 minutes . Cell pellets were collected by centrifugation ( 6,000 rpm , 5 minutes at 4 °C ) , thoroughly resuspended in 100 μl of lysozyme solution [ 2 mg/ml in TE buffer ( 10 mM Tris-HCl and 1 mM EDTA ) ] , and incubated for 2 minutes . The cells were immediately lysed by adding 1 ml of TRIzol Reagent ( Invitrogen , 15596 ) and the RNA was extracted following manufacture 's instruction . For the ChIP-seq samples , the E. coli cells were fixed with 1 % formaldehyde with continued shaking at <Temp> 37 °C </Temp> for 10 minutes before quenching with glycine ( 100 mM final concentration ) . Cells were then lysed and DNA was sheared by sonication followed by the treatment with micrococcal nuclease and RNase A. Antibody against RNAP β was used for target DNA enrichment . GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566395 RNA-seq WT log phase related to total SEnd-seq WT log phase rep 6 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the RNA-seq samples, the E. coli cells were quenched by adding 0.5 volume of cold Stop Buffer (5% phenol in ethanol) immediately to the culture medium before harvest and stay on the ice for 15 minutes. Cell pellets were collected by centrifugation (6,000 rpm, 5 minutes at 4 °C), thoroughly resuspended in 100 μl of lysozyme solution [2 mg/ml in TE buffer (10 mM Tris-HCl and 1 mM EDTA)], and incubated for 2 minutes. The cells were immediately lysed by adding 1 ml of TRIzol Reagent (Invitrogen, 15596) and the RNA was extracted following manufacture’s instruction. For the ChIP-seq samples, the E. coli cells were fixed with 1% formaldehyde with continued shaking at 37 °C for 10 minutes before quenching with glycine (100 mM final concentration). Cells were then lysed and DNA was sheared by sonication followed by the treatment with micrococcal nuclease and RNase A. Antibody against RNAP β was used for target DNA enrichment. PGCGROWTHCONDITIONS SRR8449237 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Escherichia coli K-12 MG1655 were cultured in <Med> LB media </Med> ( 10 g/L tryptone , 5 g/L yeast extract , 10 g/L NaCl , pH 7.4 ) under aerobic conditions at <Temp> 37 °C </Temp> . The temperature-sensitive RNase E mutant E.coli strain [ rne-3071 ( ts ) ] was cultured in <Med> LB media </Med> at 28 °C for normal culture and shift to 44 °C to deactive RNase E activity . GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566395 RNA-seq WT log phase related to total SEnd-seq WT log phase rep 6 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Escherichia coli K-12 MG1655 were cultured in LB media (10 g/L tryptone, 5 g/L yeast extract, 10 g/L NaCl, pH 7.4) under aerobic conditions at 37 °C. The temperature-sensitive RNase E mutant E.coli strain [rne-3071(ts)] was cultured in LB media at 28 °C for normal culture and shift to 44 °C to deactive RNase E activity. PGCGROWTHCONDITIONS SRR8449237 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C <Gtype> Wild type </Gtype> E. coli , pnp knoctout , rnb knoctout and rnr knoct out strains were cultured in <Med> LB media </Med> at <Temp> 37 °C </Temp> , RNA samples and ChIP-seq samples were collected when the growth stage of indicated cells reached to log phase or stationary phase . rne-3071 ( ts ) mutation strain was cultured in <Med> LB media </Med> at 28 °C to retain the RNase E activity or shifted to 44 °C in water bath for 30 minutes to deactivate RNase E . In order to <Gtype> inactive the rho activity , the wild type ( WT ) Wild type </Gtype> E. coli was cultured in <Med> LB media </Med> with 50 μg/ml bicyclomycin at <Temp> 37 °C </Temp> for 15 min at indicated growth condition . GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566395 RNA-seq WT log phase related to total SEnd-seq WT log phase rep 6 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Wild type E. coli, pnp knoctout, rnb knoctout and rnr knoct out strains were cultured in LB media at 37 °C, RNA samples and ChIP-seq samples were collected when the growth stage of indicated cells reached to log phase or stationary phase. rne-3071(ts) mutation strain was cultured in LB media at 28 °C to retain the RNase E activity or shifted to 44 °C in water bath for 30 minutes to deactivate RNase E. In order to inactive the rho activity, the wild type (WT) Wild type E. coli was cultured in LB media with 50 μg/ml bicyclomycin at 37 °C for 15 min at indicated growth condition. PGCGROWTHCONDITIONS SRR8449237 MCO000003199 37 °C Temperature True
characteristics 35.7 C treatment : <Temp> 37 °C </Temp> culture GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566395 RNA-seq WT log phase related to total SEnd-seq WT log phase rep 6 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 37°C culture PGCGROWTHCONDITIONS SRR8449237 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C For the RNA-seq samples , the E. coli cells were quenched by adding 0.5 volume of cold Stop Buffer ( 5 % phenol in ethanol ) immediately to the culture medium before harvest and stay on the ice for 15 minutes . Cell pellets were collected by centrifugation ( 6,000 rpm , 5 minutes at 4 °C ) , thoroughly resuspended in 100 μl of lysozyme solution [ 2 mg/ml in TE buffer ( 10 mM Tris-HCl and 1 mM EDTA ) ] , and incubated for 2 minutes . The cells were immediately lysed by adding 1 ml of TRIzol Reagent ( Invitrogen , 15596 ) and the RNA was extracted following manufacture 's instruction . For the ChIP-seq samples , the E. coli cells were fixed with 1 % formaldehyde with continued shaking at <Temp> 37 °C </Temp> for 10 minutes before quenching with glycine ( 100 mM final concentration ) . Cells were then lysed and DNA was sheared by sonication followed by the treatment with micrococcal nuclease and RNase A. Antibody against RNAP β was used for target DNA enrichment . GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566396 RNA-seq WT log phase related to total SEnd-seq WT stationary phase rep4 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the RNA-seq samples, the E. coli cells were quenched by adding 0.5 volume of cold Stop Buffer (5% phenol in ethanol) immediately to the culture medium before harvest and stay on the ice for 15 minutes. Cell pellets were collected by centrifugation (6,000 rpm, 5 minutes at 4 °C), thoroughly resuspended in 100 μl of lysozyme solution [2 mg/ml in TE buffer (10 mM Tris-HCl and 1 mM EDTA)], and incubated for 2 minutes. The cells were immediately lysed by adding 1 ml of TRIzol Reagent (Invitrogen, 15596) and the RNA was extracted following manufacture’s instruction. For the ChIP-seq samples, the E. coli cells were fixed with 1% formaldehyde with continued shaking at 37 °C for 10 minutes before quenching with glycine (100 mM final concentration). Cells were then lysed and DNA was sheared by sonication followed by the treatment with micrococcal nuclease and RNase A. Antibody against RNAP β was used for target DNA enrichment. PGCGROWTHCONDITIONS SRR8449238 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Escherichia coli K-12 MG1655 were cultured in <Med> LB media </Med> ( 10 g/L tryptone , 5 g/L yeast extract , 10 g/L NaCl , pH 7.4 ) under aerobic conditions at <Temp> 37 °C </Temp> . The temperature-sensitive RNase E mutant E.coli strain [ rne-3071 ( ts ) ] was cultured in <Med> LB media </Med> at 28 °C for normal culture and shift to 44 °C to deactive RNase E activity . GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566396 RNA-seq WT log phase related to total SEnd-seq WT stationary phase rep4 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Escherichia coli K-12 MG1655 were cultured in LB media (10 g/L tryptone, 5 g/L yeast extract, 10 g/L NaCl, pH 7.4) under aerobic conditions at 37 °C. The temperature-sensitive RNase E mutant E.coli strain [rne-3071(ts)] was cultured in LB media at 28 °C for normal culture and shift to 44 °C to deactive RNase E activity. PGCGROWTHCONDITIONS SRR8449238 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C <Gtype> Wild type </Gtype> E. coli , pnp knoctout , rnb knoctout and rnr knoct out strains were cultured in <Med> LB media </Med> at <Temp> 37 °C </Temp> , RNA samples and ChIP-seq samples were collected when the growth stage of indicated cells reached to log phase or stationary phase . rne-3071 ( ts ) mutation strain was cultured in <Med> LB media </Med> at 28 °C to retain the RNase E activity or shifted to 44 °C in water bath for 30 minutes to deactivate RNase E . In order to <Gtype> inactive the rho activity , the wild type ( WT ) Wild type </Gtype> E. coli was cultured in <Med> LB media </Med> with 50 μg/ml bicyclomycin at <Temp> 37 °C </Temp> for 15 min at indicated growth condition . GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566396 RNA-seq WT log phase related to total SEnd-seq WT stationary phase rep4 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Wild type E. coli, pnp knoctout, rnb knoctout and rnr knoct out strains were cultured in LB media at 37 °C, RNA samples and ChIP-seq samples were collected when the growth stage of indicated cells reached to log phase or stationary phase. rne-3071(ts) mutation strain was cultured in LB media at 28 °C to retain the RNase E activity or shifted to 44 °C in water bath for 30 minutes to deactivate RNase E. In order to inactive the rho activity, the wild type (WT) Wild type E. coli was cultured in LB media with 50 μg/ml bicyclomycin at 37 °C for 15 min at indicated growth condition. PGCGROWTHCONDITIONS SRR8449238 MCO000003199 37 °C Temperature True
characteristics 35.7 C treatment : <Temp> 37 °C </Temp> culture GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566396 RNA-seq WT log phase related to total SEnd-seq WT stationary phase rep4 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 37°C culture PGCGROWTHCONDITIONS SRR8449238 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL17024-GPL17439-GPL18995-GPL25769 GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655). GPL18995: Illumina MiSeq (Escherichia coli BW25113). GPL25769: Illumina Genome Analyzer IIx (Escherichia coli BW25113) GSE122211 Expression profiling of E. coli K-12 MG1655 GSM3461156 MG_no_te_1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122211/GSE122211.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8164476 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL17024-GPL17439-GPL18995-GPL25769 GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655). GPL18995: Illumina MiSeq (Escherichia coli BW25113). GPL25769: Illumina Genome Analyzer IIx (Escherichia coli BW25113) GSE122211 Expression profiling of E. coli K-12 MG1655 GSM3461157 MG_no_te_2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122211/GSE122211.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8164477 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL17024-GPL17439-GPL18995-GPL25769 GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655). GPL18995: Illumina MiSeq (Escherichia coli BW25113). GPL25769: Illumina Genome Analyzer IIx (Escherichia coli BW25113) GSE122211 Expression profiling of E. coli K-12 MG1655 GSM3461164 MG_glu_1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122211/GSE122211.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8164484 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL17024-GPL17439-GPL18995-GPL25769 GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655). GPL18995: Illumina MiSeq (Escherichia coli BW25113). GPL25769: Illumina Genome Analyzer IIx (Escherichia coli BW25113) GSE122211 Expression profiling of E. coli K-12 MG1655 GSM3461165 MG_glu_2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122211/GSE122211.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8164485 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL17024-GPL17439-GPL18995-GPL25769 GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655). GPL18995: Illumina MiSeq (Escherichia coli BW25113). GPL25769: Illumina Genome Analyzer IIx (Escherichia coli BW25113) GSE122211 Expression profiling of E. coli K-12 MG1655 GSM3461166 MG_glc 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122211/GSE122211.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8164486 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463565 wt_glc__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8173227 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463566 wt_glc__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8173228 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463567 wt_glc__3 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8173229 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463568 wt_glc__4 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8173230 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463569 arg_sbt__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8173231 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463570 arg_sbt__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8173232 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463571 cytd_rib__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8173233 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463572 cytd_rib__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8173234 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463573 gth__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8173235 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463574 gth__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8173236 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463575 leu_glcr__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8173237 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463576 leu_glcr__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8173238 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463577 met_glc__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8173239 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463578 met_glc__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8173240 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463579 no3_anaero__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8173241 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463580 no3_anaero__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8173242 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463581 phe_acgam__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8173243 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463582 phe_acgam__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8173244 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463583 thm_gal__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8173245 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463584 thm_gal__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8173246 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463585 tyr_glcn__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8173247 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463586 tyr_glcn__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8173248 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463587 ura_pyr__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8173249 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463588 ura_pyr__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8173250 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3854833 wt_glc_5__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR9204648 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3854834 wt_glc_6__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR9204649 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3854835 bw_delpurR_cytd__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR9204650 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3854836 bw_delpurR_cytd__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR9204651 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3854837 ade_glc__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR9204652 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3854838 ade_glc__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR9204653 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE122296 Expression profiling of multiple Escherichia coli strains on glucose minimal media GSM3463601 MG1655_1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122296/GSE122296.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8173221 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C After inoculation and growth , 3 mL of cell broth ( OD600 ~ 0.5 ) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent ( 6 mL ) , vortexed for 5 seconds , incubated at room temperature for 5 min , and immediately centrifuged for 10 min at 17,500 RPMs . The supernatant was decanted , and the cell pellet was stored in the -80 °C . Cell pellets were thawed and incubated with Readylyse Lysozyme , SuperaseIn , Protease K , and 20 % SDS for 20 minutes at <Temp> 37 °C </Temp> . Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures . An on-column DNase-treatment was performed for 30 minutes at room temperature . RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer . The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria . A KAPA Stranded RNA-Seq Kit ( Kapa Biosystems KK8401 ) was used following the manufacturer 's protocol to create sequencing libraries with an average insert length of around ~ 300 bp . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE122296 Expression profiling of multiple Escherichia coli strains on glucose minimal media GSM3463602 MG1655_2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122296/GSE122296.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt After inoculation and growth, 3 mL of cell broth (OD600 ~ 0.5) was immediately added to 2 volumes Qiagen RNA-protect Bacteria Reagent (6 mL), vortexed for 5 seconds, incubated at room temperature for 5 min, and immediately centrifuged for 10 min at 17,500 RPMs. The supernatant was decanted, and the cell pellet was stored in the -80°C. Cell pellets were thawed and incubated with Readylyse Lysozyme, SuperaseIn, Protease K, and 20% SDS for 20 minutes at 37°C. Total RNA was isolated and purified using the Qiagen RNeasy Mini Kit columns and following vendor procedures. An on-column DNase-treatment was performed for 30 minutes at room temperature. RNA was quantified using a Nano drop and quality assessed by running an RNA-nano chip on a bioanalyzer. The rRNA was removed using Illumina Ribo-Zero rRNA removal kit for Gram Negative Bacteria. A KAPA Stranded RNA-Seq Kit (Kapa Biosystems KK8401) was used following the manufacturer’s protocol to create sequencing libraries with an average insert length of around ~300 bp. PGCGROWTHCONDITIONS SRR8173222 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , Δfis and Δhns were grown in Luria-Bertani ( LB ) broth and agar ( 15g/l ) . Where needed , kanamycin was used at final concentration of 50µg/ml . For RNA extraction , E. coli cultures were grown to mid-exponential growth phase <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with 40 % ( w/v ) glucose . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE123554 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GSM3507068 delta-fis rep1 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE123554/GSE123554.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, Δfis and Δhns were grown in Luria-Bertani (LB) broth and agar (15g/l). Where needed, kanamycin was used at final concentration of 50µg/ml. For RNA extraction, E. coli cultures were grown to mid-exponential growth phase aerobically at 37°C in M9 minimal media supplemented with 40% (w/v) glucose. PGCGROWTHCONDITIONS SRR8309841 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , Δfis and Δhns were grown in Luria-Bertani ( LB ) broth and agar ( 15g/l ) . Where needed , kanamycin was used at final concentration of 50µg/ml . For RNA extraction , E. coli cultures were grown to mid-exponential growth phase <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with 40 % ( w/v ) glucose . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE123554 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GSM3507069 delta-fis rep2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE123554/GSE123554.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, Δfis and Δhns were grown in Luria-Bertani (LB) broth and agar (15g/l). Where needed, kanamycin was used at final concentration of 50µg/ml. For RNA extraction, E. coli cultures were grown to mid-exponential growth phase aerobically at 37°C in M9 minimal media supplemented with 40% (w/v) glucose. PGCGROWTHCONDITIONS SRR8309842 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , Δfis and Δhns were grown in Luria-Bertani ( LB ) broth and agar ( 15g/l ) . Where needed , kanamycin was used at final concentration of 50µg/ml . For RNA extraction , E. coli cultures were grown to mid-exponential growth phase <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with 40 % ( w/v ) glucose . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE123554 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GSM3507070 delta-hns rep1 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE123554/GSE123554.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, Δfis and Δhns were grown in Luria-Bertani (LB) broth and agar (15g/l). Where needed, kanamycin was used at final concentration of 50µg/ml. For RNA extraction, E. coli cultures were grown to mid-exponential growth phase aerobically at 37°C in M9 minimal media supplemented with 40% (w/v) glucose. PGCGROWTHCONDITIONS SRR8309843 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , Δfis and Δhns were grown in Luria-Bertani ( LB ) broth and agar ( 15g/l ) . Where needed , kanamycin was used at final concentration of 50µg/ml . For RNA extraction , E. coli cultures were grown to mid-exponential growth phase <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with 40 % ( w/v ) glucose . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE123554 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GSM3507071 delta-hns rep2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE123554/GSE123554.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, Δfis and Δhns were grown in Luria-Bertani (LB) broth and agar (15g/l). Where needed, kanamycin was used at final concentration of 50µg/ml. For RNA extraction, E. coli cultures were grown to mid-exponential growth phase aerobically at 37°C in M9 minimal media supplemented with 40% (w/v) glucose. PGCGROWTHCONDITIONS SRR8309844 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C Total RNA was extracted using the Trizol reagent ( Thermo Fisher Scientific ) and the concentration and purity of the samples were determined using Nano Drop . RNA integrity was monitored using the R6K ScreenTape system on the Agilent 2200 TapeStation . gDNA was removed by Turbo DNase ( Thermo Fisher Scientific ) in the presence of RiboLock RNase Inhibitor ( Thermo Fisher Scientific ) for 60 min at <Temp> 37 °C </Temp> . Following organic extraction ( 1x 25:24:1 v/v phenol/chloroform / isoamyalcohol , 1x chloroform ) , RNA was recovered by overnight precipitation with 3 volumes of 30:1 100 % ethanol/3 M sodium acetate ( pH 5.2 ) . The RNA preparations were further treated with RiboZero ( Illumina ) and the concentration of the samples was determined with Qubit . GPL26204 GPL26204: NextSeq 550 (Escherichia coli K-12) GSE126710 RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GSM3611666 E. coli K-12 MG1655_R1 [MG_1] 31208335 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE126710/GSE126710.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Total RNA was extracted using the Trizol reagent (Thermo Fisher Scientific) and the concentration and purity of the samples were determined using Nano Drop. RNA integrity was monitored using the R6K ScreenTape system on the Agilent 2200 TapeStation. gDNA was removed by Turbo DNase (Thermo Fisher Scientific) in the presence of RiboLock RNase Inhibitor (Thermo Fisher Scientific) for 60 min at 37°C. Following organic extraction (1x 25:24:1 v/v phenol/chloroform/ isoamyalcohol, 1x chloroform), RNA was recovered by overnight precipitation with 3 volumes of 30:1 100 % ethanol/3 M sodium acetate (pH 5.2). The RNA preparations were further treated with RiboZero (Illumina) and the concentration of the samples was determined with Qubit. PGCGROWTHCONDITIONS SRR8587784 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C Total RNA was extracted using the Trizol reagent ( Thermo Fisher Scientific ) and the concentration and purity of the samples were determined using Nano Drop . RNA integrity was monitored using the R6K ScreenTape system on the Agilent 2200 TapeStation . gDNA was removed by Turbo DNase ( Thermo Fisher Scientific ) in the presence of RiboLock RNase Inhibitor ( Thermo Fisher Scientific ) for 60 min at <Temp> 37 °C </Temp> . Following organic extraction ( 1x 25:24:1 v/v phenol/chloroform / isoamyalcohol , 1x chloroform ) , RNA was recovered by overnight precipitation with 3 volumes of 30:1 100 % ethanol/3 M sodium acetate ( pH 5.2 ) . The RNA preparations were further treated with RiboZero ( Illumina ) and the concentration of the samples was determined with Qubit . GPL26204 GPL26204: NextSeq 550 (Escherichia coli K-12) GSE126710 RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GSM3611667 E. coli K-12 MG1655_R2 [MG_2] 31208335 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE126710/GSE126710.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Total RNA was extracted using the Trizol reagent (Thermo Fisher Scientific) and the concentration and purity of the samples were determined using Nano Drop. RNA integrity was monitored using the R6K ScreenTape system on the Agilent 2200 TapeStation. gDNA was removed by Turbo DNase (Thermo Fisher Scientific) in the presence of RiboLock RNase Inhibitor (Thermo Fisher Scientific) for 60 min at 37°C. Following organic extraction (1x 25:24:1 v/v phenol/chloroform/ isoamyalcohol, 1x chloroform), RNA was recovered by overnight precipitation with 3 volumes of 30:1 100 % ethanol/3 M sodium acetate (pH 5.2). The RNA preparations were further treated with RiboZero (Illumina) and the concentration of the samples was determined with Qubit. PGCGROWTHCONDITIONS SRR8587785 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C Total RNA was extracted using the Trizol reagent ( Thermo Fisher Scientific ) and the concentration and purity of the samples were determined using Nano Drop . RNA integrity was monitored using the R6K ScreenTape system on the Agilent 2200 TapeStation . gDNA was removed by Turbo DNase ( Thermo Fisher Scientific ) in the presence of RiboLock RNase Inhibitor ( Thermo Fisher Scientific ) for 60 min at <Temp> 37 °C </Temp> . Following organic extraction ( 1x 25:24:1 v/v phenol/chloroform / isoamyalcohol , 1x chloroform ) , RNA was recovered by overnight precipitation with 3 volumes of 30:1 100 % ethanol/3 M sodium acetate ( pH 5.2 ) . The RNA preparations were further treated with RiboZero ( Illumina ) and the concentration of the samples was determined with Qubit . GPL26204 GPL26204: NextSeq 550 (Escherichia coli K-12) GSE126710 RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GSM3611668 E. coli K-12 MG1655_R3 [MG_3] 31208335 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE126710/GSE126710.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Total RNA was extracted using the Trizol reagent (Thermo Fisher Scientific) and the concentration and purity of the samples were determined using Nano Drop. RNA integrity was monitored using the R6K ScreenTape system on the Agilent 2200 TapeStation. gDNA was removed by Turbo DNase (Thermo Fisher Scientific) in the presence of RiboLock RNase Inhibitor (Thermo Fisher Scientific) for 60 min at 37°C. Following organic extraction (1x 25:24:1 v/v phenol/chloroform/ isoamyalcohol, 1x chloroform), RNA was recovered by overnight precipitation with 3 volumes of 30:1 100 % ethanol/3 M sodium acetate (pH 5.2). The RNA preparations were further treated with RiboZero (Illumina) and the concentration of the samples was determined with Qubit. PGCGROWTHCONDITIONS SRR8587786 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C Total RNA was extracted using the Trizol reagent ( Thermo Fisher Scientific ) and the concentration and purity of the samples were determined using Nano Drop . RNA integrity was monitored using the R6K ScreenTape system on the Agilent 2200 TapeStation . gDNA was removed by Turbo DNase ( Thermo Fisher Scientific ) in the presence of RiboLock RNase Inhibitor ( Thermo Fisher Scientific ) for 60 min at <Temp> 37 °C </Temp> . Following organic extraction ( 1x 25:24:1 v/v phenol/chloroform / isoamyalcohol , 1x chloroform ) , RNA was recovered by overnight precipitation with 3 volumes of 30:1 100 % ethanol/3 M sodium acetate ( pH 5.2 ) . The RNA preparations were further treated with RiboZero ( Illumina ) and the concentration of the samples was determined with Qubit . GPL26204 GPL26204: NextSeq 550 (Escherichia coli K-12) GSE126710 RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GSM3611669 E. coli K-12 MG1655::φO104_R1 [O104_1] 31208335 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE126710/GSE126710.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Total RNA was extracted using the Trizol reagent (Thermo Fisher Scientific) and the concentration and purity of the samples were determined using Nano Drop. RNA integrity was monitored using the R6K ScreenTape system on the Agilent 2200 TapeStation. gDNA was removed by Turbo DNase (Thermo Fisher Scientific) in the presence of RiboLock RNase Inhibitor (Thermo Fisher Scientific) for 60 min at 37°C. Following organic extraction (1x 25:24:1 v/v phenol/chloroform/ isoamyalcohol, 1x chloroform), RNA was recovered by overnight precipitation with 3 volumes of 30:1 100 % ethanol/3 M sodium acetate (pH 5.2). The RNA preparations were further treated with RiboZero (Illumina) and the concentration of the samples was determined with Qubit. PGCGROWTHCONDITIONS SRR8587787 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C Total RNA was extracted using the Trizol reagent ( Thermo Fisher Scientific ) and the concentration and purity of the samples were determined using Nano Drop . RNA integrity was monitored using the R6K ScreenTape system on the Agilent 2200 TapeStation . gDNA was removed by Turbo DNase ( Thermo Fisher Scientific ) in the presence of RiboLock RNase Inhibitor ( Thermo Fisher Scientific ) for 60 min at <Temp> 37 °C </Temp> . Following organic extraction ( 1x 25:24:1 v/v phenol/chloroform / isoamyalcohol , 1x chloroform ) , RNA was recovered by overnight precipitation with 3 volumes of 30:1 100 % ethanol/3 M sodium acetate ( pH 5.2 ) . The RNA preparations were further treated with RiboZero ( Illumina ) and the concentration of the samples was determined with Qubit . GPL26204 GPL26204: NextSeq 550 (Escherichia coli K-12) GSE126710 RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GSM3611670 E. coli K-12 MG1655::φO104_R2 [O104_2] 31208335 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE126710/GSE126710.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Total RNA was extracted using the Trizol reagent (Thermo Fisher Scientific) and the concentration and purity of the samples were determined using Nano Drop. RNA integrity was monitored using the R6K ScreenTape system on the Agilent 2200 TapeStation. gDNA was removed by Turbo DNase (Thermo Fisher Scientific) in the presence of RiboLock RNase Inhibitor (Thermo Fisher Scientific) for 60 min at 37°C. Following organic extraction (1x 25:24:1 v/v phenol/chloroform/ isoamyalcohol, 1x chloroform), RNA was recovered by overnight precipitation with 3 volumes of 30:1 100 % ethanol/3 M sodium acetate (pH 5.2). The RNA preparations were further treated with RiboZero (Illumina) and the concentration of the samples was determined with Qubit. PGCGROWTHCONDITIONS SRR8587788 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C Total RNA was extracted using the Trizol reagent ( Thermo Fisher Scientific ) and the concentration and purity of the samples were determined using Nano Drop . RNA integrity was monitored using the R6K ScreenTape system on the Agilent 2200 TapeStation . gDNA was removed by Turbo DNase ( Thermo Fisher Scientific ) in the presence of RiboLock RNase Inhibitor ( Thermo Fisher Scientific ) for 60 min at <Temp> 37 °C </Temp> . Following organic extraction ( 1x 25:24:1 v/v phenol/chloroform / isoamyalcohol , 1x chloroform ) , RNA was recovered by overnight precipitation with 3 volumes of 30:1 100 % ethanol/3 M sodium acetate ( pH 5.2 ) . The RNA preparations were further treated with RiboZero ( Illumina ) and the concentration of the samples was determined with Qubit . GPL26204 GPL26204: NextSeq 550 (Escherichia coli K-12) GSE126710 RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GSM3611671 E. coli K-12 MG1655::φO104_R3 [O104_3] 31208335 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE126710/GSE126710.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Total RNA was extracted using the Trizol reagent (Thermo Fisher Scientific) and the concentration and purity of the samples were determined using Nano Drop. RNA integrity was monitored using the R6K ScreenTape system on the Agilent 2200 TapeStation. gDNA was removed by Turbo DNase (Thermo Fisher Scientific) in the presence of RiboLock RNase Inhibitor (Thermo Fisher Scientific) for 60 min at 37°C. Following organic extraction (1x 25:24:1 v/v phenol/chloroform/ isoamyalcohol, 1x chloroform), RNA was recovered by overnight precipitation with 3 volumes of 30:1 100 % ethanol/3 M sodium acetate (pH 5.2). The RNA preparations were further treated with RiboZero (Illumina) and the concentration of the samples was determined with Qubit. PGCGROWTHCONDITIONS SRR8587789 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C Total RNA was extracted using the Trizol reagent ( Thermo Fisher Scientific ) and the concentration and purity of the samples were determined using Nano Drop . RNA integrity was monitored using the R6K ScreenTape system on the Agilent 2200 TapeStation . gDNA was removed by Turbo DNase ( Thermo Fisher Scientific ) in the presence of RiboLock RNase Inhibitor ( Thermo Fisher Scientific ) for 60 min at <Temp> 37 °C </Temp> . Following organic extraction ( 1x 25:24:1 v/v phenol/chloroform / isoamyalcohol , 1x chloroform ) , RNA was recovered by overnight precipitation with 3 volumes of 30:1 100 % ethanol/3 M sodium acetate ( pH 5.2 ) . The RNA preparations were further treated with RiboZero ( Illumina ) and the concentration of the samples was determined with Qubit . GPL26204 GPL26204: NextSeq 550 (Escherichia coli K-12) GSE126710 RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GSM3611672 E. coli K-12 MG1655_φPA8_R1 [PA8_1] 31208335 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE126710/GSE126710.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Total RNA was extracted using the Trizol reagent (Thermo Fisher Scientific) and the concentration and purity of the samples were determined using Nano Drop. RNA integrity was monitored using the R6K ScreenTape system on the Agilent 2200 TapeStation. gDNA was removed by Turbo DNase (Thermo Fisher Scientific) in the presence of RiboLock RNase Inhibitor (Thermo Fisher Scientific) for 60 min at 37°C. Following organic extraction (1x 25:24:1 v/v phenol/chloroform/ isoamyalcohol, 1x chloroform), RNA was recovered by overnight precipitation with 3 volumes of 30:1 100 % ethanol/3 M sodium acetate (pH 5.2). The RNA preparations were further treated with RiboZero (Illumina) and the concentration of the samples was determined with Qubit. PGCGROWTHCONDITIONS SRR8587790 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C Total RNA was extracted using the Trizol reagent ( Thermo Fisher Scientific ) and the concentration and purity of the samples were determined using Nano Drop . RNA integrity was monitored using the R6K ScreenTape system on the Agilent 2200 TapeStation . gDNA was removed by Turbo DNase ( Thermo Fisher Scientific ) in the presence of RiboLock RNase Inhibitor ( Thermo Fisher Scientific ) for 60 min at <Temp> 37 °C </Temp> . Following organic extraction ( 1x 25:24:1 v/v phenol/chloroform / isoamyalcohol , 1x chloroform ) , RNA was recovered by overnight precipitation with 3 volumes of 30:1 100 % ethanol/3 M sodium acetate ( pH 5.2 ) . The RNA preparations were further treated with RiboZero ( Illumina ) and the concentration of the samples was determined with Qubit . GPL26204 GPL26204: NextSeq 550 (Escherichia coli K-12) GSE126710 RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GSM3611673 E. coli K-12 MG1655_φPA8_R2 [PA8_2] 31208335 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE126710/GSE126710.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Total RNA was extracted using the Trizol reagent (Thermo Fisher Scientific) and the concentration and purity of the samples were determined using Nano Drop. RNA integrity was monitored using the R6K ScreenTape system on the Agilent 2200 TapeStation. gDNA was removed by Turbo DNase (Thermo Fisher Scientific) in the presence of RiboLock RNase Inhibitor (Thermo Fisher Scientific) for 60 min at 37°C. Following organic extraction (1x 25:24:1 v/v phenol/chloroform/ isoamyalcohol, 1x chloroform), RNA was recovered by overnight precipitation with 3 volumes of 30:1 100 % ethanol/3 M sodium acetate (pH 5.2). The RNA preparations were further treated with RiboZero (Illumina) and the concentration of the samples was determined with Qubit. PGCGROWTHCONDITIONS SRR8587791 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C Total RNA was extracted using the Trizol reagent ( Thermo Fisher Scientific ) and the concentration and purity of the samples were determined using Nano Drop . RNA integrity was monitored using the R6K ScreenTape system on the Agilent 2200 TapeStation . gDNA was removed by Turbo DNase ( Thermo Fisher Scientific ) in the presence of RiboLock RNase Inhibitor ( Thermo Fisher Scientific ) for 60 min at <Temp> 37 °C </Temp> . Following organic extraction ( 1x 25:24:1 v/v phenol/chloroform / isoamyalcohol , 1x chloroform ) , RNA was recovered by overnight precipitation with 3 volumes of 30:1 100 % ethanol/3 M sodium acetate ( pH 5.2 ) . The RNA preparations were further treated with RiboZero ( Illumina ) and the concentration of the samples was determined with Qubit . GPL26204 GPL26204: NextSeq 550 (Escherichia coli K-12) GSE126710 RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GSM3611674 E. coli K-12 MG1655_fastφPA8_R3 [PA8_3] 31208335 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE126710/GSE126710.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Total RNA was extracted using the Trizol reagent (Thermo Fisher Scientific) and the concentration and purity of the samples were determined using Nano Drop. RNA integrity was monitored using the R6K ScreenTape system on the Agilent 2200 TapeStation. gDNA was removed by Turbo DNase (Thermo Fisher Scientific) in the presence of RiboLock RNase Inhibitor (Thermo Fisher Scientific) for 60 min at 37°C. Following organic extraction (1x 25:24:1 v/v phenol/chloroform/ isoamyalcohol, 1x chloroform), RNA was recovered by overnight precipitation with 3 volumes of 30:1 100 % ethanol/3 M sodium acetate (pH 5.2). The RNA preparations were further treated with RiboZero (Illumina) and the concentration of the samples was determined with Qubit. PGCGROWTHCONDITIONS SRR8587792 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275442 WT_LB_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907640 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275443 WT_LB_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907641 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275444 WT_EtOH_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907642 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275446 BaeR_KO_LB_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907644 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275447 BaeR_KO_ LB_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907645 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275448 BaeR_KO_EtOH_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907646 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275450 CpxR_KO_LB_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907648 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275451 CpxR_KO_LB_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907649 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275454 KdpE_KO_01-KCl_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907652 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275455 KdpE_KO_01-KCl_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907653 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275456 KdpE_KO_115-KCl_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907654 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275457 KdpE_KO_115-KCl_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907655 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275458 WT_01-KCl_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907656 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275459 WT_01-KCl_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907657 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275460 WT_115-KCl_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907658 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275461 WT_115-KCl_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907659 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275462 PhoB_KO_M9_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907660 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275463 PhoB_KO_M9_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907661 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275464 PhoB_KO_M9-P_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907662 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275465 PhoB_KO_M9-P_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907663 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275466 WT_M9-P_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907664 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275467 WT_M9-P_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907665 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275469 ZraR_KO_LB_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907667 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275470 ZraR_KO_ZnCl2_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907668 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275471 ZraR_KO_ZnCl2_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907669 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275472 WT_ZnCl2_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907670 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275473 WT_ZnCl2_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907671 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C An overnight culture was diluted to an <OD> OD600 of 0.02 </OD> in <Med> LB media </Med> supplemented with 100 µg/ml of carbenicillin , 40 µg/ml biotin , and <Supp> 70 µM IPTG </Supp> . The back diluted culture was grown at <Temp> 37 °C </Temp> until reaching an OD600 of 0.45 . GPL10328 GPL10328: Illumina Genome Analyzer II (Escherichia coli) GSE33671 Selective ribosome profiling reveals the co-translational chaperone action of trigger factor in vivo GSM832606 DSP1_AP 22153074 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE33671/GSE33671.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt An overnight culture was diluted to an OD600 of 0.02 in LB media supplemented with 100 µg/ml of carbenicillin, 40 µg/ml biotin, and 70 µM IPTG. The back diluted culture was grown at 37°C until reaching an OD600 of 0.45. PGCGROWTHCONDITIONS SRR364363 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C An overnight culture was diluted to an <OD> OD600 of 0.02 </OD> in <Med> LB media </Med> supplemented with 100 µg/ml of carbenicillin , 40 µg/ml biotin , and <Supp> 70 µM IPTG </Supp> . The back diluted culture was grown at <Temp> 37 °C </Temp> until reaching an OD600 of 0.45 . GPL10328 GPL10328: Illumina Genome Analyzer II (Escherichia coli) GSE33671 Selective ribosome profiling reveals the co-translational chaperone action of trigger factor in vivo GSM832607 DSP1_Total 22153074 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE33671/GSE33671.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt An overnight culture was diluted to an OD600 of 0.02 in LB media supplemented with 100 µg/ml of carbenicillin, 40 µg/ml biotin, and 70 µM IPTG. The back diluted culture was grown at 37°C until reaching an OD600 of 0.45. PGCGROWTHCONDITIONS SRR364364 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C An overnight culture was diluted to an <OD> OD600 of 0.02 </OD> in <Med> LB media </Med> supplemented with 100 µg/ml of carbenicillin , 40 µg/ml biotin , and <Supp> 70 µM IPTG </Supp> . The back diluted culture was grown at <Temp> 37 °C </Temp> until reaching an OD600 of 0.45 . GPL10328 GPL10328: Illumina Genome Analyzer II (Escherichia coli) GSE33671 Selective ribosome profiling reveals the co-translational chaperone action of trigger factor in vivo GSM832608 DSP2_AP 22153074 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE33671/GSE33671.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt An overnight culture was diluted to an OD600 of 0.02 in LB media supplemented with 100 µg/ml of carbenicillin, 40 µg/ml biotin, and 70 µM IPTG. The back diluted culture was grown at 37°C until reaching an OD600 of 0.45. PGCGROWTHCONDITIONS SRR364365 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C An overnight culture was diluted to an <OD> OD600 of 0.02 </OD> in <Med> LB media </Med> supplemented with 100 µg/ml of carbenicillin , 40 µg/ml biotin , and <Supp> 70 µM IPTG </Supp> . The back diluted culture was grown at <Temp> 37 °C </Temp> until reaching an OD600 of 0.45 . GPL10328 GPL10328: Illumina Genome Analyzer II (Escherichia coli) GSE33671 Selective ribosome profiling reveals the co-translational chaperone action of trigger factor in vivo GSM832609 DSP2_Total 22153074 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE33671/GSE33671.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt An overnight culture was diluted to an OD600 of 0.02 in LB media supplemented with 100 µg/ml of carbenicillin, 40 µg/ml biotin, and 70 µM IPTG. The back diluted culture was grown at 37°C until reaching an OD600 of 0.45. PGCGROWTHCONDITIONS SRR364366 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C An overnight culture was diluted to an <OD> OD600 of 0.02 </OD> in <Med> LB media </Med> supplemented with 100 µg/ml of carbenicillin , 40 µg/ml biotin , and <Supp> 70 µM IPTG </Supp> . The back diluted culture was grown at <Temp> 37 °C </Temp> until reaching an OD600 of 0.45 . GPL10328 GPL10328: Illumina Genome Analyzer II (Escherichia coli) GSE33671 Selective ribosome profiling reveals the co-translational chaperone action of trigger factor in vivo GSM832610 DSP3_AP 22153074 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE33671/GSE33671.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt An overnight culture was diluted to an OD600 of 0.02 in LB media supplemented with 100 µg/ml of carbenicillin, 40 µg/ml biotin, and 70 µM IPTG. The back diluted culture was grown at 37°C until reaching an OD600 of 0.45. PGCGROWTHCONDITIONS SRR364367 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C An overnight culture was diluted to an <OD> OD600 of 0.02 </OD> in <Med> LB media </Med> supplemented with 100 µg/ml of carbenicillin , 40 µg/ml biotin , and <Supp> 70 µM IPTG </Supp> . The back diluted culture was grown at <Temp> 37 °C </Temp> until reaching an OD600 of 0.45 . GPL10328 GPL10328: Illumina Genome Analyzer II (Escherichia coli) GSE33671 Selective ribosome profiling reveals the co-translational chaperone action of trigger factor in vivo GSM832611 DSP3_Total 22153074 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE33671/GSE33671.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt An overnight culture was diluted to an OD600 of 0.02 in LB media supplemented with 100 µg/ml of carbenicillin, 40 µg/ml biotin, and 70 µM IPTG. The back diluted culture was grown at 37°C until reaching an OD600 of 0.45. PGCGROWTHCONDITIONS SRR364368 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C An overnight culture was diluted to an <OD> OD600 of 0.02 </OD> in <Med> LB media </Med> supplemented with 100 µg/ml of carbenicillin , 40 µg/ml biotin , and <Supp> 70 µM IPTG </Supp> . The back diluted culture was grown at <Temp> 37 °C </Temp> until reaching an OD600 of 0.45 . GPL10328 GPL10328: Illumina Genome Analyzer II (Escherichia coli) GSE33671 Selective ribosome profiling reveals the co-translational chaperone action of trigger factor in vivo GSM832612 EDC1_AP 22153074 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE33671/GSE33671.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt An overnight culture was diluted to an OD600 of 0.02 in LB media supplemented with 100 µg/ml of carbenicillin, 40 µg/ml biotin, and 70 µM IPTG. The back diluted culture was grown at 37°C until reaching an OD600 of 0.45. PGCGROWTHCONDITIONS SRR364369 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C An overnight culture was diluted to an <OD> OD600 of 0.02 </OD> in <Med> LB media </Med> supplemented with 100 µg/ml of carbenicillin , 40 µg/ml biotin , and <Supp> 70 µM IPTG </Supp> . The back diluted culture was grown at <Temp> 37 °C </Temp> until reaching an OD600 of 0.45 . GPL10328 GPL10328: Illumina Genome Analyzer II (Escherichia coli) GSE33671 Selective ribosome profiling reveals the co-translational chaperone action of trigger factor in vivo GSM832613 EDC1_Total 22153074 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE33671/GSE33671.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt An overnight culture was diluted to an OD600 of 0.02 in LB media supplemented with 100 µg/ml of carbenicillin, 40 µg/ml biotin, and 70 µM IPTG. The back diluted culture was grown at 37°C until reaching an OD600 of 0.45. PGCGROWTHCONDITIONS SRR364370 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C Total RNA was isolated with TRIzol ( invitrogen , Carlsbad , CA ) according to the manufacture [ linebreak ] s instruction . DNA was removed from RNA extracts with RNase-free DNase I ( New England Biolabs , Beverly , MA , USA ) by incubation at <Temp> 37 °C </Temp> for <Supp> 30 min </Supp> . The quality of total RNA was assessed by using 2100 Bioanalyzer ( Agilent , Santa Clara , USA ) and also checked by agarose gel electrophoresis . rRNAs was subsquently removed from the total mRNA following the instruction of the Ribo-Zero rRNATM Removal kit ( Epicentre , Madison , WI , USA ) . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE43408 Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition. GSM1217967 pHerd30T CK+aerobic 23856776 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE43408/GSE43408.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Total RNA was isolated with TRIzol (invitrogen, Carlsbad, CA) according to the manufacture[linebreak]s instruction. DNA was removed from RNA extracts with RNase-free DNase I (New England Biolabs, Beverly, MA, USA) by incubation at 37°C for 30 min. The quality of total RNA was assessed by using 2100 Bioanalyzer (Agilent, Santa Clara, USA) and also checked by agarose gel electrophoresis. rRNAs was subsquently removed from the total mRNA following the instruction of the Ribo-Zero rRNATM Removal kit (Epicentre, Madison, WI, USA). PGCGROWTHCONDITIONS SRR958658 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C Total RNA was isolated with TRIzol ( invitrogen , Carlsbad , CA ) according to the manufacture [ linebreak ] s instruction . DNA was removed from RNA extracts with RNase-free DNase I ( New England Biolabs , Beverly , MA , USA ) by incubation at <Temp> 37 °C </Temp> for <Supp> 30 min </Supp> . The quality of total RNA was assessed by using 2100 Bioanalyzer ( Agilent , Santa Clara , USA ) and also checked by agarose gel electrophoresis . rRNAs was subsquently removed from the total mRNA following the instruction of the Ribo-Zero rRNATM Removal kit ( Epicentre , Madison , WI , USA ) . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE43408 Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition. GSM1217968 pHerd30T-LL37 induced+aerobic 23856776 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE43408/GSE43408.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Total RNA was isolated with TRIzol (invitrogen, Carlsbad, CA) according to the manufacture[linebreak]s instruction. DNA was removed from RNA extracts with RNase-free DNase I (New England Biolabs, Beverly, MA, USA) by incubation at 37°C for 30 min. The quality of total RNA was assessed by using 2100 Bioanalyzer (Agilent, Santa Clara, USA) and also checked by agarose gel electrophoresis. rRNAs was subsquently removed from the total mRNA following the instruction of the Ribo-Zero rRNATM Removal kit (Epicentre, Madison, WI, USA). PGCGROWTHCONDITIONS SRR958659 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C Total RNA was isolated with TRIzol ( invitrogen , Carlsbad , CA ) according to the manufacture [ linebreak ] s instruction . DNA was removed from RNA extracts with RNase-free DNase I ( New England Biolabs , Beverly , MA , USA ) by incubation at <Temp> 37 °C </Temp> for <Supp> 30 min </Supp> . The quality of total RNA was assessed by using 2100 Bioanalyzer ( Agilent , Santa Clara , USA ) and also checked by agarose gel electrophoresis . rRNAs was subsquently removed from the total mRNA following the instruction of the Ribo-Zero rRNATM Removal kit ( Epicentre , Madison , WI , USA ) . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE43408 Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition. GSM1217969 pHerd30T-LL37 CK+ anaerobic 23856776 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE43408/GSE43408.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Total RNA was isolated with TRIzol (invitrogen, Carlsbad, CA) according to the manufacture[linebreak]s instruction. DNA was removed from RNA extracts with RNase-free DNase I (New England Biolabs, Beverly, MA, USA) by incubation at 37°C for 30 min. The quality of total RNA was assessed by using 2100 Bioanalyzer (Agilent, Santa Clara, USA) and also checked by agarose gel electrophoresis. rRNAs was subsquently removed from the total mRNA following the instruction of the Ribo-Zero rRNATM Removal kit (Epicentre, Madison, WI, USA). PGCGROWTHCONDITIONS SRR958660 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C Total RNA was isolated with TRIzol ( invitrogen , Carlsbad , CA ) according to the manufacture [ linebreak ] s instruction . DNA was removed from RNA extracts with RNase-free DNase I ( New England Biolabs , Beverly , MA , USA ) by incubation at <Temp> 37 °C </Temp> for <Supp> 30 min </Supp> . The quality of total RNA was assessed by using 2100 Bioanalyzer ( Agilent , Santa Clara , USA ) and also checked by agarose gel electrophoresis . rRNAs was subsquently removed from the total mRNA following the instruction of the Ribo-Zero rRNATM Removal kit ( Epicentre , Madison , WI , USA ) . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE43408 Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition. GSM1217970 pHerd30T-LL37 induced +anaerobic 23856776 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE43408/GSE43408.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Total RNA was isolated with TRIzol (invitrogen, Carlsbad, CA) according to the manufacture[linebreak]s instruction. DNA was removed from RNA extracts with RNase-free DNase I (New England Biolabs, Beverly, MA, USA) by incubation at 37°C for 30 min. The quality of total RNA was assessed by using 2100 Bioanalyzer (Agilent, Santa Clara, USA) and also checked by agarose gel electrophoresis. rRNAs was subsquently removed from the total mRNA following the instruction of the Ribo-Zero rRNATM Removal kit (Epicentre, Madison, WI, USA). PGCGROWTHCONDITIONS SRR958661 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104381 pHDB3_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794827 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104386 pLCV1_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794832 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104387 MG1655-aMG_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794833 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104388 MG1655-aMG_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794834 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104389 MG1655-aMG_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794835 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104390 MG1655+aMG_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794836 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104391 MG1655+aMG_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794837 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104392 MG1655+aMG_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794838 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104393 SgrR_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794839 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104394 SgrR_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794840 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104395 SgrR_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794841 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104396 sgrS_T_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794842 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104397 sgrS_T_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794843 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104398 sgrS_T_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794844 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104399 sgrS_un_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794845 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104400 sgrS_un_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794846 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104401 sgrS_un_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794847 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104402 WT_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794848 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104403 WT_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794849 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104404 WT_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794850 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104405 wt_T_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794851 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104406 wt_T_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794852 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104407 wt_T_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794853 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104408 wt_un_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794854 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104410 wt_un_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794856 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104411 CV108_minus_aMG_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794857 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104412 CV108_minus_aMG_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794858 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104413 CV108_minus_aMG_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794859 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104414 CV108_plus_aMG_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794860 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104415 CV108_plus_aMG_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794861 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104416 CV108_plus_aMG_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794862 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104417 MG1655_minus_aMG_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794863 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104418 MG1655_minus_aMG_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794864 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104419 MG1655_minus_aMG_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794865 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104420 MG1655_plus_aMG_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794866 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104421 MG1655_plus_aMG_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794867 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104422 MG1655_plus_aMG_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794868 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104423 WT_minus_2DG_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794869 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104424 WT_minus_2DG_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794870 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104425 WT_minus_2DG_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794871 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104426 WT_plus_2DG_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794872 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104427 WT_plus_2DG_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794873 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104428 WT_plus_2DG_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794874 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174823 Mid log_wildtype_glc minimal media_aerobic rep1 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922260 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174824 Mid log_wildtype_glc minimal media_aerobic rep2 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922261 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174825 Mid log_wildtype_glc minimal media_aerobic rep3 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922262 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174826 Mid log_wildtype_glc minimal media + adenine_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922263 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174827 Mid log_wildtype_glc minimal media + L-tryptophan_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922264 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174828 Mid log_wildtype_glc minimal media_anaerobic rep1 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922265 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174829 Mid log_wildtype_glc minimal media_anaerobic rep2 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922266 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174830 Mid log_wildtype_glc minimal media_anaerobic rep3 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922267 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174831 Mid log_nac KO_glc minimal media_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922268 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174832 Mid log_nac KO_glc minimal media + adenine_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922269 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174833 Mid log_cra KO_glc minimal media_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922270 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174834 Mid log_cra KO_glc minimal media + L-tryptophan_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922271 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174835 Mid log_mntR KO_glc minimal media_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922272 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174836 Mid log_mntR KO_glc minimal media_anaerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922273 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT and Δfur were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . For iron treated cells , 0.1 mM of FeCl2 were treated from the beginning of culture , and for DPD treated cells , <Supp> 0.2 mM of DPD </Supp> were added at early-log phase and continued to culture for additional <Supp> 2h </Supp> . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326347 WT with Fe 1 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. PGCGROWTHCONDITIONS SRR1168133 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT and Δfur were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . For iron treated cells , 0.1 mM of FeCl2 were treated from the beginning of culture , and for DPD treated cells , <Supp> 0.2 mM of DPD </Supp> were added at early-log phase and continued to culture for additional <Supp> 2h </Supp> . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326348 WT with Fe 2 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. PGCGROWTHCONDITIONS SRR1168134 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT and Δfur were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . For iron treated cells , 0.1 mM of FeCl2 were treated from the beginning of culture , and for DPD treated cells , <Supp> 0.2 mM of DPD </Supp> were added at early-log phase and continued to culture for additional <Supp> 2h </Supp> . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326349 WT with DPD 1 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. PGCGROWTHCONDITIONS SRR1168135 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT and Δfur were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . For iron treated cells , 0.1 mM of FeCl2 were treated from the beginning of culture , and for DPD treated cells , <Supp> 0.2 mM of DPD </Supp> were added at early-log phase and continued to culture for additional <Supp> 2h </Supp> . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326350 WT with DPD 2 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. PGCGROWTHCONDITIONS SRR1168136 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT and Δfur were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . For iron treated cells , 0.1 mM of FeCl2 were treated from the beginning of culture , and for DPD treated cells , <Supp> 0.2 mM of DPD </Supp> were added at early-log phase and continued to culture for additional <Supp> 2h </Supp> . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326351 Δfur with Fe 1 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. PGCGROWTHCONDITIONS SRR1168137 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT and Δfur were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . For iron treated cells , 0.1 mM of FeCl2 were treated from the beginning of culture , and for DPD treated cells , <Supp> 0.2 mM of DPD </Supp> were added at early-log phase and continued to culture for additional <Supp> 2h </Supp> . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326352 Δfur with Fe 2 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. PGCGROWTHCONDITIONS SRR1168138 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT and Δfur were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . For iron treated cells , 0.1 mM of FeCl2 were treated from the beginning of culture , and for DPD treated cells , <Supp> 0.2 mM of DPD </Supp> were added at early-log phase and continued to culture for additional <Supp> 2h </Supp> . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326353 Δfur with DPD 1 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. PGCGROWTHCONDITIONS SRR1168139 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT and Δfur were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . For iron treated cells , 0.1 mM of FeCl2 were treated from the beginning of culture , and for DPD treated cells , <Supp> 0.2 mM of DPD </Supp> were added at early-log phase and continued to culture for additional <Supp> 2h </Supp> . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326354 Δfur with DPD 2 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. PGCGROWTHCONDITIONS SRR1168140 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Cultures were grown aerobically at <Temp> 37 °C </Temp> in 1 L volumes of M9 minimal medium , supplemented with MgSO4 ( 1 mM ) , CaCl2 ( 0.1 mM ) , and glucose ( 10 g/L ) in vigorously shaken ( 225 rpm ) Fernbach flasks . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360031 Wild-type (MG1655) T0 RNA rep 2 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cultures were grown aerobically at 37 °C in 1 L volumes of M9 minimal medium, supplemented with MgSO4 (1 mM), CaCl2 (0.1 mM), and glucose (10 g/L) in vigorously shaken (225 rpm) Fernbach flasks. PGCGROWTHCONDITIONS SRR1211036 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Cultures were grown aerobically at <Temp> 37 °C </Temp> in 1 L volumes of M9 minimal medium , supplemented with MgSO4 ( 1 mM ) , CaCl2 ( 0.1 mM ) , and glucose ( 10 g/L ) in vigorously shaken ( 225 rpm ) Fernbach flasks . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360032 Wild-type (MG1655) T1 RNA rep 1 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cultures were grown aerobically at 37 °C in 1 L volumes of M9 minimal medium, supplemented with MgSO4 (1 mM), CaCl2 (0.1 mM), and glucose (10 g/L) in vigorously shaken (225 rpm) Fernbach flasks. PGCGROWTHCONDITIONS SRR1211037 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Cultures were grown aerobically at <Temp> 37 °C </Temp> in 1 L volumes of M9 minimal medium , supplemented with MgSO4 ( 1 mM ) , CaCl2 ( 0.1 mM ) , and glucose ( 10 g/L ) in vigorously shaken ( 225 rpm ) Fernbach flasks . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360033 Wild-type (MG1655) T1 RNA rep 2 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cultures were grown aerobically at 37 °C in 1 L volumes of M9 minimal medium, supplemented with MgSO4 (1 mM), CaCl2 (0.1 mM), and glucose (10 g/L) in vigorously shaken (225 rpm) Fernbach flasks. PGCGROWTHCONDITIONS SRR1211038 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Cultures were grown aerobically at <Temp> 37 °C </Temp> in 1 L volumes of M9 minimal medium , supplemented with MgSO4 ( 1 mM ) , CaCl2 ( 0.1 mM ) , and glucose ( 10 g/L ) in vigorously shaken ( 225 rpm ) Fernbach flasks . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360034 Wild-type (MG1655) T2 RNA rep 1 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cultures were grown aerobically at 37 °C in 1 L volumes of M9 minimal medium, supplemented with MgSO4 (1 mM), CaCl2 (0.1 mM), and glucose (10 g/L) in vigorously shaken (225 rpm) Fernbach flasks. PGCGROWTHCONDITIONS SRR1211039 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Cultures were grown aerobically at <Temp> 37 °C </Temp> in 1 L volumes of M9 minimal medium , supplemented with MgSO4 ( 1 mM ) , CaCl2 ( 0.1 mM ) , and glucose ( 10 g/L ) in vigorously shaken ( 225 rpm ) Fernbach flasks . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360035 Wild-type (MG1655) T2 RNA rep 2 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cultures were grown aerobically at 37 °C in 1 L volumes of M9 minimal medium, supplemented with MgSO4 (1 mM), CaCl2 (0.1 mM), and glucose (10 g/L) in vigorously shaken (225 rpm) Fernbach flasks. PGCGROWTHCONDITIONS SRR1211040 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Cultures were grown aerobically at <Temp> 37 °C </Temp> in 1 L volumes of M9 minimal medium , supplemented with MgSO4 ( 1 mM ) , CaCl2 ( 0.1 mM ) , and glucose ( 10 g/L ) in vigorously shaken ( 225 rpm ) Fernbach flasks . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360036 Mutant (EP61) T0 RNA rep 1 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cultures were grown aerobically at 37 °C in 1 L volumes of M9 minimal medium, supplemented with MgSO4 (1 mM), CaCl2 (0.1 mM), and glucose (10 g/L) in vigorously shaken (225 rpm) Fernbach flasks. PGCGROWTHCONDITIONS SRR1211041 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Cultures were grown aerobically at <Temp> 37 °C </Temp> in 1 L volumes of M9 minimal medium , supplemented with MgSO4 ( 1 mM ) , CaCl2 ( 0.1 mM ) , and glucose ( 10 g/L ) in vigorously shaken ( 225 rpm ) Fernbach flasks . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360037 Mutant (EP61) T0 RNA rep 2 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cultures were grown aerobically at 37 °C in 1 L volumes of M9 minimal medium, supplemented with MgSO4 (1 mM), CaCl2 (0.1 mM), and glucose (10 g/L) in vigorously shaken (225 rpm) Fernbach flasks. PGCGROWTHCONDITIONS SRR1211042 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Cultures were grown aerobically at <Temp> 37 °C </Temp> in 1 L volumes of M9 minimal medium , supplemented with MgSO4 ( 1 mM ) , CaCl2 ( 0.1 mM ) , and glucose ( 10 g/L ) in vigorously shaken ( 225 rpm ) Fernbach flasks . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360038 Mutant (EP61) T1 RNA rep 1 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cultures were grown aerobically at 37 °C in 1 L volumes of M9 minimal medium, supplemented with MgSO4 (1 mM), CaCl2 (0.1 mM), and glucose (10 g/L) in vigorously shaken (225 rpm) Fernbach flasks. PGCGROWTHCONDITIONS SRR1211043 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Cultures were grown aerobically at <Temp> 37 °C </Temp> in 1 L volumes of M9 minimal medium , supplemented with MgSO4 ( 1 mM ) , CaCl2 ( 0.1 mM ) , and glucose ( 10 g/L ) in vigorously shaken ( 225 rpm ) Fernbach flasks . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360039 Mutant (EP61) T1 RNA rep 2 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cultures were grown aerobically at 37 °C in 1 L volumes of M9 minimal medium, supplemented with MgSO4 (1 mM), CaCl2 (0.1 mM), and glucose (10 g/L) in vigorously shaken (225 rpm) Fernbach flasks. PGCGROWTHCONDITIONS SRR1211044 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Cultures were grown aerobically at <Temp> 37 °C </Temp> in 1 L volumes of M9 minimal medium , supplemented with MgSO4 ( 1 mM ) , CaCl2 ( 0.1 mM ) , and glucose ( 10 g/L ) in vigorously shaken ( 225 rpm ) Fernbach flasks . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360040 Mutant (EP61) T2 RNA rep 1 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cultures were grown aerobically at 37 °C in 1 L volumes of M9 minimal medium, supplemented with MgSO4 (1 mM), CaCl2 (0.1 mM), and glucose (10 g/L) in vigorously shaken (225 rpm) Fernbach flasks. PGCGROWTHCONDITIONS SRR1211045 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Cultures were grown aerobically at <Temp> 37 °C </Temp> in 1 L volumes of M9 minimal medium , supplemented with MgSO4 ( 1 mM ) , CaCl2 ( 0.1 mM ) , and glucose ( 10 g/L ) in vigorously shaken ( 225 rpm ) Fernbach flasks . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360041 Mutant (EP61) T2 RNA rep 2 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cultures were grown aerobically at 37 °C in 1 L volumes of M9 minimal medium, supplemented with MgSO4 (1 mM), CaCl2 (0.1 mM), and glucose (10 g/L) in vigorously shaken (225 rpm) Fernbach flasks. PGCGROWTHCONDITIONS SRR1211046 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Cultures were grown aerobically at <Temp> 37 °C </Temp> in 1 L volumes of M9 minimal medium , supplemented with MgSO4 ( 1 mM ) , CaCl2 ( 0.1 mM ) , and glucose ( 10 g/L ) in vigorously shaken ( 225 rpm ) Fernbach flasks . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360042 Wild-type (MG1655) T0 RP rep 1 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cultures were grown aerobically at 37 °C in 1 L volumes of M9 minimal medium, supplemented with MgSO4 (1 mM), CaCl2 (0.1 mM), and glucose (10 g/L) in vigorously shaken (225 rpm) Fernbach flasks. PGCGROWTHCONDITIONS SRR1211047 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Cultures were grown aerobically at <Temp> 37 °C </Temp> in 1 L volumes of M9 minimal medium , supplemented with MgSO4 ( 1 mM ) , CaCl2 ( 0.1 mM ) , and glucose ( 10 g/L ) in vigorously shaken ( 225 rpm ) Fernbach flasks . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360043 Wild-type (MG1655) T0 RP rep 2 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cultures were grown aerobically at 37 °C in 1 L volumes of M9 minimal medium, supplemented with MgSO4 (1 mM), CaCl2 (0.1 mM), and glucose (10 g/L) in vigorously shaken (225 rpm) Fernbach flasks. PGCGROWTHCONDITIONS SRR1211048 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Cultures were grown aerobically at <Temp> 37 °C </Temp> in 1 L volumes of M9 minimal medium , supplemented with MgSO4 ( 1 mM ) , CaCl2 ( 0.1 mM ) , and glucose ( 10 g/L ) in vigorously shaken ( 225 rpm ) Fernbach flasks . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360044 Wild-type (MG1655) T1 RP rep 1 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cultures were grown aerobically at 37 °C in 1 L volumes of M9 minimal medium, supplemented with MgSO4 (1 mM), CaCl2 (0.1 mM), and glucose (10 g/L) in vigorously shaken (225 rpm) Fernbach flasks. PGCGROWTHCONDITIONS SRR1211049 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Cultures were grown aerobically at <Temp> 37 °C </Temp> in 1 L volumes of M9 minimal medium , supplemented with MgSO4 ( 1 mM ) , CaCl2 ( 0.1 mM ) , and glucose ( 10 g/L ) in vigorously shaken ( 225 rpm ) Fernbach flasks . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360045 Wild-type (MG1655) T1 RP rep 2 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cultures were grown aerobically at 37 °C in 1 L volumes of M9 minimal medium, supplemented with MgSO4 (1 mM), CaCl2 (0.1 mM), and glucose (10 g/L) in vigorously shaken (225 rpm) Fernbach flasks. PGCGROWTHCONDITIONS SRR1211050 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Cultures were grown aerobically at <Temp> 37 °C </Temp> in 1 L volumes of M9 minimal medium , supplemented with MgSO4 ( 1 mM ) , CaCl2 ( 0.1 mM ) , and glucose ( 10 g/L ) in vigorously shaken ( 225 rpm ) Fernbach flasks . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360046 Wild-type (MG1655) T2 RP rep 1 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cultures were grown aerobically at 37 °C in 1 L volumes of M9 minimal medium, supplemented with MgSO4 (1 mM), CaCl2 (0.1 mM), and glucose (10 g/L) in vigorously shaken (225 rpm) Fernbach flasks. PGCGROWTHCONDITIONS SRR1211051 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Cultures were grown aerobically at <Temp> 37 °C </Temp> in 1 L volumes of M9 minimal medium , supplemented with MgSO4 ( 1 mM ) , CaCl2 ( 0.1 mM ) , and glucose ( 10 g/L ) in vigorously shaken ( 225 rpm ) Fernbach flasks . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360047 Wild-type (MG1655) T2 RP rep 2 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cultures were grown aerobically at 37 °C in 1 L volumes of M9 minimal medium, supplemented with MgSO4 (1 mM), CaCl2 (0.1 mM), and glucose (10 g/L) in vigorously shaken (225 rpm) Fernbach flasks. PGCGROWTHCONDITIONS SRR1211052 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Cultures were grown aerobically at <Temp> 37 °C </Temp> in 1 L volumes of M9 minimal medium , supplemented with MgSO4 ( 1 mM ) , CaCl2 ( 0.1 mM ) , and glucose ( 10 g/L ) in vigorously shaken ( 225 rpm ) Fernbach flasks . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360048 Mutant (EP61) T0 RP rep 1 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cultures were grown aerobically at 37 °C in 1 L volumes of M9 minimal medium, supplemented with MgSO4 (1 mM), CaCl2 (0.1 mM), and glucose (10 g/L) in vigorously shaken (225 rpm) Fernbach flasks. PGCGROWTHCONDITIONS SRR1211053 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Cultures were grown aerobically at <Temp> 37 °C </Temp> in 1 L volumes of M9 minimal medium , supplemented with MgSO4 ( 1 mM ) , CaCl2 ( 0.1 mM ) , and glucose ( 10 g/L ) in vigorously shaken ( 225 rpm ) Fernbach flasks . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360049 Mutant (EP61) T0 RP rep 2 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cultures were grown aerobically at 37 °C in 1 L volumes of M9 minimal medium, supplemented with MgSO4 (1 mM), CaCl2 (0.1 mM), and glucose (10 g/L) in vigorously shaken (225 rpm) Fernbach flasks. PGCGROWTHCONDITIONS SRR1211054 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Cultures were grown aerobically at <Temp> 37 °C </Temp> in 1 L volumes of M9 minimal medium , supplemented with MgSO4 ( 1 mM ) , CaCl2 ( 0.1 mM ) , and glucose ( 10 g/L ) in vigorously shaken ( 225 rpm ) Fernbach flasks . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360050 Mutant (EP61) T1 RP rep 1 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cultures were grown aerobically at 37 °C in 1 L volumes of M9 minimal medium, supplemented with MgSO4 (1 mM), CaCl2 (0.1 mM), and glucose (10 g/L) in vigorously shaken (225 rpm) Fernbach flasks. PGCGROWTHCONDITIONS SRR1211055 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Cultures were grown aerobically at <Temp> 37 °C </Temp> in 1 L volumes of M9 minimal medium , supplemented with MgSO4 ( 1 mM ) , CaCl2 ( 0.1 mM ) , and glucose ( 10 g/L ) in vigorously shaken ( 225 rpm ) Fernbach flasks . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360051 Mutant (EP61) T1 RP rep 2 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cultures were grown aerobically at 37 °C in 1 L volumes of M9 minimal medium, supplemented with MgSO4 (1 mM), CaCl2 (0.1 mM), and glucose (10 g/L) in vigorously shaken (225 rpm) Fernbach flasks. PGCGROWTHCONDITIONS SRR1211056 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Cultures were grown aerobically at <Temp> 37 °C </Temp> in 1 L volumes of M9 minimal medium , supplemented with MgSO4 ( 1 mM ) , CaCl2 ( 0.1 mM ) , and glucose ( 10 g/L ) in vigorously shaken ( 225 rpm ) Fernbach flasks . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360052 Mutant (EP61) T2 RP rep 1 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cultures were grown aerobically at 37 °C in 1 L volumes of M9 minimal medium, supplemented with MgSO4 (1 mM), CaCl2 (0.1 mM), and glucose (10 g/L) in vigorously shaken (225 rpm) Fernbach flasks. PGCGROWTHCONDITIONS SRR1211057 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Cultures were grown aerobically at <Temp> 37 °C </Temp> in 1 L volumes of M9 minimal medium , supplemented with MgSO4 ( 1 mM ) , CaCl2 ( 0.1 mM ) , and glucose ( 10 g/L ) in vigorously shaken ( 225 rpm ) Fernbach flasks . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360053 Mutant (EP61) T2 RP rep 2 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cultures were grown aerobically at 37 °C in 1 L volumes of M9 minimal medium, supplemented with MgSO4 (1 mM), CaCl2 (0.1 mM), and glucose (10 g/L) in vigorously shaken (225 rpm) Fernbach flasks. PGCGROWTHCONDITIONS SRR1211058 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All cultures grown up to 0.6 OD600 with shaking at 200 rpm . Samples 1,2,5 and 6 were grown at 30 °C whereas samples 3 and 4 were grown at <Temp> 37 °C </Temp> . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE58285 Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli GSM1405877 rne wild-type 25237058 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE58285/GSE58285.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All cultures grown up to 0.6 OD600 with shaking at 200 rpm. Samples 1,2,5 and 6 were grown at 30°C whereas samples 3 and 4 were grown at 37°C. PGCGROWTHCONDITIONS SRR1363864 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All cultures grown up to 0.6 OD600 with shaking at 200 rpm . Samples 1,2,5 and 6 were grown at 30 °C whereas samples 3 and 4 were grown at <Temp> 37 °C </Temp> . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE58285 Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli GSM1405878 rne-3071 ts 25237058 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE58285/GSE58285.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All cultures grown up to 0.6 OD600 with shaking at 200 rpm. Samples 1,2,5 and 6 were grown at 30°C whereas samples 3 and 4 were grown at 37°C. PGCGROWTHCONDITIONS SRR1363865 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All cultures grown up to 0.6 OD600 with shaking at 200 rpm . Samples 1,2,5 and 6 were grown at 30 °C whereas samples 3 and 4 were grown at <Temp> 37 °C </Temp> . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE58285 Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli GSM1405879 T170V 0 min 25237058 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE58285/GSE58285.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All cultures grown up to 0.6 OD600 with shaking at 200 rpm. Samples 1,2,5 and 6 were grown at 30°C whereas samples 3 and 4 were grown at 37°C. PGCGROWTHCONDITIONS SRR1363866 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All cultures grown up to 0.6 OD600 with shaking at 200 rpm . Samples 1,2,5 and 6 were grown at 30 °C whereas samples 3 and 4 were grown at <Temp> 37 °C </Temp> . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE58285 Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli GSM1405880 T170V 10 min 25237058 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE58285/GSE58285.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All cultures grown up to 0.6 OD600 with shaking at 200 rpm. Samples 1,2,5 and 6 were grown at 30°C whereas samples 3 and 4 were grown at 37°C. PGCGROWTHCONDITIONS SRR1363867 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All cultures grown up to 0.6 OD600 with shaking at 200 rpm . Samples 1,2,5 and 6 were grown at 30 °C whereas samples 3 and 4 were grown at <Temp> 37 °C </Temp> . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE58285 Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli GSM1405882 rng mutant 25237058 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE58285/GSE58285.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All cultures grown up to 0.6 OD600 with shaking at 200 rpm. Samples 1,2,5 and 6 were grown at 30°C whereas samples 3 and 4 were grown at 37°C. PGCGROWTHCONDITIONS SRR1363869 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Gtype> Wild type </Gtype> E. coli K-12 ( strain BW38038 ) and BW39452 ( ΔrpoS ) cultures were grown on <Med> MOPS glucose minimal medium </Med> with <Supp> 0.2 % glucose </Supp> as sole carbon source at <Temp> 37 °C </Temp> , pH was initially 7.4 , and the agitation speed was 500 rpm . Culture samples were harvested during logarithmic growth and following entry into stationary phase for the WT and rpoS mutant . OD600 measurements were made on a Beckman Coulter DU 800 spectrophotometer . Samples were harvested directly into ice-cold RNAlater at a 1:1 dilution to protect RNA from degradation and cells then were pelleted by centrifugation at 8000rpm for 10 minutes . Cell pellets were stored at -80 °C in an equal volume of RNAlater prior to RNA extraction . GPL18814 GPL18814: Illumina HiSeq 2000 (Escherichia coli BW38028) GSE58556 RNA sequencing based analysis of the bacterial transcriptome GSM1413874 WT_glucose_log 25483350 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE58556/GSE58556.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Wild type E. coli K-12 (strain BW38038) and BW39452(ΔrpoS) cultures were grown on MOPS glucose minimal medium with 0.2% glucose as sole carbon source at 37°C, pH was initially 7.4, and the agitation speed was 500 rpm. Culture samples were harvested during logarithmic growth and following entry into stationary phase for the WT and rpoS mutant. OD600 measurements were made on a Beckman Coulter DU 800 spectrophotometer. Samples were harvested directly into ice-cold RNAlater at a 1:1 dilution to protect RNA from degradation and cells then were pelleted by centrifugation at 8000rpm for 10 minutes. Cell pellets were stored at -80°C in an equal volume of RNAlater prior to RNA extraction. PGCGROWTHCONDITIONS SRR1411272 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C Total RNA was extracted using TRIzol reagent ( Invitrogen ) and the sample was enriched in mRNA , depleting small RNAs with GeneJET ™ RNA Purification Kit ( Fermentas ) and ribosomal RNA with two cycle of MICROBExpress ™ Bacterial mRNA Enrichment Kit ( Ambion ) . To probe the RNA structure two µg of enriched mRNA were resuspended in 45 µl of DEPC water and denatured for 3 min at 95 °C , refolded at <Temp> 37 °C </Temp> , after addition of 10x RNA-structure buffer with pH 7.0 ( 100 mM Tris , 1 M KCl , 100 mM MgCl2 ) and digested for 1 min at <Temp> 37 °C </Temp> with either 0.05 U RNase V1 ( Life Technologies ) or a combination of 2 µg RNase A and 5 U RNase T1 ( Thermo Scientific ) . The reaction was stopped by extracting the RNA with phenol-chlorophorm . The RNase A/T1-digested sample was phosphorylated with T4 PNK ( NEB ) and purified with RNA Clean & Concentrator ™ kit ( Zymo Research ) . Both the V1 and A/T1 digested samples were randomly fragmented in buffer with pH 9.2 ( 100 mM Na2CO3 , 2 mM EDTA ) for 12 min at 95 °C . GPL14548-GPL18945 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18945: Illumina Genome Analyzer IIx (Escherichia coli) GSE63817 Secondary structure across the bacterial transcriptome reveals versatile roles in mRNA regulation and function GSM1558078 LB mRNA 26495981 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE63817/GSE63817.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Total RNA was extracted using TRIzol reagent (Invitrogen) and the sample was enriched in mRNA, depleting small RNAs with GeneJET™ RNA Purification Kit (Fermentas) and ribosomal RNA with two cycle of MICROBExpress™Bacterial mRNA Enrichment Kit (Ambion). To probe the RNA structure two µg of enriched mRNA were resuspended in 45 µl of DEPC water and denatured for 3 min at 95°C,refolded at 37 °C, after addition of 10x RNA-structure buffer with pH 7.0 (100 mM Tris, 1 M KCl, 100 mM MgCl2) and digested for 1 min at 37 °C with either 0.05 U RNase V1 (Life Technologies) or a combination of 2 µg RNase A and 5 U RNase T1 (Thermo Scientific). The reaction was stopped by extracting the RNA with phenol-chlorophorm. The RNase A/T1-digested sample was phosphorylated with T4 PNK (NEB) and purified with RNA Clean & Concentrator™ kit (Zymo Research). Both the V1 and A/T1 digested samples were randomly fragmented in buffer with pH 9.2 (100 mM Na2CO3, 2 mM EDTA) for 12 min at 95°C. PGCGROWTHCONDITIONS SRR1692165 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli MC4100 strain was cultured at <Temp> 37 °C </Temp> to <Phase> mid-log phase </Phase> ( OD600 ~ 0.4 ) in <Med> LB media </Med> GPL14548-GPL18945 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18945: Illumina Genome Analyzer IIx (Escherichia coli) GSE63817 Secondary structure across the bacterial transcriptome reveals versatile roles in mRNA regulation and function GSM1558078 LB mRNA 26495981 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE63817/GSE63817.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli MC4100 strain was cultured at 37°C to mid-log phase (OD600 ~ 0.4) in LB media PGCGROWTHCONDITIONS SRR1692165 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C Total RNA was extracted using TRIzol reagent ( Invitrogen ) and the sample was enriched in mRNA , depleting small RNAs with GeneJET ™ RNA Purification Kit ( Fermentas ) and ribosomal RNA with two cycle of MICROBExpress ™ Bacterial mRNA Enrichment Kit ( Ambion ) . To probe the RNA structure two µg of enriched mRNA were resuspended in 45 µl of DEPC water and denatured for 3 min at 95 °C , refolded at <Temp> 37 °C </Temp> , after addition of 10x RNA-structure buffer with pH 7.0 ( 100 mM Tris , 1 M KCl , 100 mM MgCl2 ) and digested for 1 min at <Temp> 37 °C </Temp> with either 0.05 U RNase V1 ( Life Technologies ) or a combination of 2 µg RNase A and 5 U RNase T1 ( Thermo Scientific ) . The reaction was stopped by extracting the RNA with phenol-chlorophorm . The RNase A/T1-digested sample was phosphorylated with T4 PNK ( NEB ) and purified with RNA Clean & Concentrator ™ kit ( Zymo Research ) . Both the V1 and A/T1 digested samples were randomly fragmented in buffer with pH 9.2 ( 100 mM Na2CO3 , 2 mM EDTA ) for 12 min at 95 °C . GPL14548-GPL18945 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18945: Illumina Genome Analyzer IIx (Escherichia coli) GSE63817 Secondary structure across the bacterial transcriptome reveals versatile roles in mRNA regulation and function GSM1558079 LB RPF 26495981 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE63817/GSE63817.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Total RNA was extracted using TRIzol reagent (Invitrogen) and the sample was enriched in mRNA, depleting small RNAs with GeneJET™ RNA Purification Kit (Fermentas) and ribosomal RNA with two cycle of MICROBExpress™Bacterial mRNA Enrichment Kit (Ambion). To probe the RNA structure two µg of enriched mRNA were resuspended in 45 µl of DEPC water and denatured for 3 min at 95°C,refolded at 37 °C, after addition of 10x RNA-structure buffer with pH 7.0 (100 mM Tris, 1 M KCl, 100 mM MgCl2) and digested for 1 min at 37 °C with either 0.05 U RNase V1 (Life Technologies) or a combination of 2 µg RNase A and 5 U RNase T1 (Thermo Scientific). The reaction was stopped by extracting the RNA with phenol-chlorophorm. The RNase A/T1-digested sample was phosphorylated with T4 PNK (NEB) and purified with RNA Clean & Concentrator™ kit (Zymo Research). Both the V1 and A/T1 digested samples were randomly fragmented in buffer with pH 9.2 (100 mM Na2CO3, 2 mM EDTA) for 12 min at 95°C. PGCGROWTHCONDITIONS SRR1692166 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli MC4100 strain was cultured at <Temp> 37 °C </Temp> to <Phase> mid-log phase </Phase> ( OD600 ~ 0.4 ) in <Med> LB media </Med> GPL14548-GPL18945 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18945: Illumina Genome Analyzer IIx (Escherichia coli) GSE63817 Secondary structure across the bacterial transcriptome reveals versatile roles in mRNA regulation and function GSM1558079 LB RPF 26495981 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE63817/GSE63817.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli MC4100 strain was cultured at 37°C to mid-log phase (OD600 ~ 0.4) in LB media PGCGROWTHCONDITIONS SRR1692166 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C Total RNA was extracted using TRIzol reagent ( Invitrogen ) and the sample was enriched in mRNA , depleting small RNAs with GeneJET ™ RNA Purification Kit ( Fermentas ) and ribosomal RNA with two cycle of MICROBExpress ™ Bacterial mRNA Enrichment Kit ( Ambion ) . To probe the RNA structure two µg of enriched mRNA were resuspended in 45 µl of DEPC water and denatured for 3 min at 95 °C , refolded at <Temp> 37 °C </Temp> , after addition of 10x RNA-structure buffer with pH 7.0 ( 100 mM Tris , 1 M KCl , 100 mM MgCl2 ) and digested for 1 min at <Temp> 37 °C </Temp> with either 0.05 U RNase V1 ( Life Technologies ) or a combination of 2 µg RNase A and 5 U RNase T1 ( Thermo Scientific ) . The reaction was stopped by extracting the RNA with phenol-chlorophorm . The RNase A/T1-digested sample was phosphorylated with T4 PNK ( NEB ) and purified with RNA Clean & Concentrator ™ kit ( Zymo Research ) . Both the V1 and A/T1 digested samples were randomly fragmented in buffer with pH 9.2 ( 100 mM Na2CO3 , 2 mM EDTA ) for 12 min at 95 °C . GPL14548-GPL18945 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18945: Illumina Genome Analyzer IIx (Escherichia coli) GSE63817 Secondary structure across the bacterial transcriptome reveals versatile roles in mRNA regulation and function GSM1558080 LB mRNA technical replicate 26495981 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE63817/GSE63817.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Total RNA was extracted using TRIzol reagent (Invitrogen) and the sample was enriched in mRNA, depleting small RNAs with GeneJET™ RNA Purification Kit (Fermentas) and ribosomal RNA with two cycle of MICROBExpress™Bacterial mRNA Enrichment Kit (Ambion). To probe the RNA structure two µg of enriched mRNA were resuspended in 45 µl of DEPC water and denatured for 3 min at 95°C,refolded at 37 °C, after addition of 10x RNA-structure buffer with pH 7.0 (100 mM Tris, 1 M KCl, 100 mM MgCl2) and digested for 1 min at 37 °C with either 0.05 U RNase V1 (Life Technologies) or a combination of 2 µg RNase A and 5 U RNase T1 (Thermo Scientific). The reaction was stopped by extracting the RNA with phenol-chlorophorm. The RNase A/T1-digested sample was phosphorylated with T4 PNK (NEB) and purified with RNA Clean & Concentrator™ kit (Zymo Research). Both the V1 and A/T1 digested samples were randomly fragmented in buffer with pH 9.2 (100 mM Na2CO3, 2 mM EDTA) for 12 min at 95°C. PGCGROWTHCONDITIONS SRR1692167 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli MC4100 strain was cultured at <Temp> 37 °C </Temp> to <Phase> mid-log phase </Phase> ( OD600 ~ 0.4 ) in <Med> LB media </Med> GPL14548-GPL18945 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18945: Illumina Genome Analyzer IIx (Escherichia coli) GSE63817 Secondary structure across the bacterial transcriptome reveals versatile roles in mRNA regulation and function GSM1558080 LB mRNA technical replicate 26495981 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE63817/GSE63817.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli MC4100 strain was cultured at 37°C to mid-log phase (OD600 ~ 0.4) in LB media PGCGROWTHCONDITIONS SRR1692167 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C Total RNA was extracted using TRIzol reagent ( Invitrogen ) and the sample was enriched in mRNA , depleting small RNAs with GeneJET ™ RNA Purification Kit ( Fermentas ) and ribosomal RNA with two cycle of MICROBExpress ™ Bacterial mRNA Enrichment Kit ( Ambion ) . To probe the RNA structure two µg of enriched mRNA were resuspended in 45 µl of DEPC water and denatured for 3 min at 95 °C , refolded at <Temp> 37 °C </Temp> , after addition of 10x RNA-structure buffer with pH 7.0 ( 100 mM Tris , 1 M KCl , 100 mM MgCl2 ) and digested for 1 min at <Temp> 37 °C </Temp> with either 0.05 U RNase V1 ( Life Technologies ) or a combination of 2 µg RNase A and 5 U RNase T1 ( Thermo Scientific ) . The reaction was stopped by extracting the RNA with phenol-chlorophorm . The RNase A/T1-digested sample was phosphorylated with T4 PNK ( NEB ) and purified with RNA Clean & Concentrator ™ kit ( Zymo Research ) . Both the V1 and A/T1 digested samples were randomly fragmented in buffer with pH 9.2 ( 100 mM Na2CO3 , 2 mM EDTA ) for 12 min at 95 °C . GPL14548-GPL18945 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18945: Illumina Genome Analyzer IIx (Escherichia coli) GSE63817 Secondary structure across the bacterial transcriptome reveals versatile roles in mRNA regulation and function GSM1558081 LB RPF biological replicate 26495981 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE63817/GSE63817.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Total RNA was extracted using TRIzol reagent (Invitrogen) and the sample was enriched in mRNA, depleting small RNAs with GeneJET™ RNA Purification Kit (Fermentas) and ribosomal RNA with two cycle of MICROBExpress™Bacterial mRNA Enrichment Kit (Ambion). To probe the RNA structure two µg of enriched mRNA were resuspended in 45 µl of DEPC water and denatured for 3 min at 95°C,refolded at 37 °C, after addition of 10x RNA-structure buffer with pH 7.0 (100 mM Tris, 1 M KCl, 100 mM MgCl2) and digested for 1 min at 37 °C with either 0.05 U RNase V1 (Life Technologies) or a combination of 2 µg RNase A and 5 U RNase T1 (Thermo Scientific). The reaction was stopped by extracting the RNA with phenol-chlorophorm. The RNase A/T1-digested sample was phosphorylated with T4 PNK (NEB) and purified with RNA Clean & Concentrator™ kit (Zymo Research). Both the V1 and A/T1 digested samples were randomly fragmented in buffer with pH 9.2 (100 mM Na2CO3, 2 mM EDTA) for 12 min at 95°C. PGCGROWTHCONDITIONS SRR1692168 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli MC4100 strain was cultured at <Temp> 37 °C </Temp> to <Phase> mid-log phase </Phase> ( OD600 ~ 0.4 ) in <Med> LB media </Med> GPL14548-GPL18945 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18945: Illumina Genome Analyzer IIx (Escherichia coli) GSE63817 Secondary structure across the bacterial transcriptome reveals versatile roles in mRNA regulation and function GSM1558081 LB RPF biological replicate 26495981 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE63817/GSE63817.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli MC4100 strain was cultured at 37°C to mid-log phase (OD600 ~ 0.4) in LB media PGCGROWTHCONDITIONS SRR1692168 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C Total RNA was extracted using TRIzol reagent ( Invitrogen ) and the sample was enriched in mRNA , depleting small RNAs with GeneJET ™ RNA Purification Kit ( Fermentas ) and ribosomal RNA with two cycle of MICROBExpress ™ Bacterial mRNA Enrichment Kit ( Ambion ) . To probe the RNA structure two µg of enriched mRNA were resuspended in 45 µl of DEPC water and denatured for 3 min at 95 °C , refolded at <Temp> 37 °C </Temp> , after addition of 10x RNA-structure buffer with pH 7.0 ( 100 mM Tris , 1 M KCl , 100 mM MgCl2 ) and digested for 1 min at <Temp> 37 °C </Temp> with either 0.05 U RNase V1 ( Life Technologies ) or a combination of 2 µg RNase A and 5 U RNase T1 ( Thermo Scientific ) . The reaction was stopped by extracting the RNA with phenol-chlorophorm . The RNase A/T1-digested sample was phosphorylated with T4 PNK ( NEB ) and purified with RNA Clean & Concentrator ™ kit ( Zymo Research ) . Both the V1 and A/T1 digested samples were randomly fragmented in buffer with pH 9.2 ( 100 mM Na2CO3 , 2 mM EDTA ) for 12 min at 95 °C . GPL14548-GPL18945 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18945: Illumina Genome Analyzer IIx (Escherichia coli) GSE63817 Secondary structure across the bacterial transcriptome reveals versatile roles in mRNA regulation and function GSM1558082 LB mRNA biological replicate 26495981 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE63817/GSE63817.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Total RNA was extracted using TRIzol reagent (Invitrogen) and the sample was enriched in mRNA, depleting small RNAs with GeneJET™ RNA Purification Kit (Fermentas) and ribosomal RNA with two cycle of MICROBExpress™Bacterial mRNA Enrichment Kit (Ambion). To probe the RNA structure two µg of enriched mRNA were resuspended in 45 µl of DEPC water and denatured for 3 min at 95°C,refolded at 37 °C, after addition of 10x RNA-structure buffer with pH 7.0 (100 mM Tris, 1 M KCl, 100 mM MgCl2) and digested for 1 min at 37 °C with either 0.05 U RNase V1 (Life Technologies) or a combination of 2 µg RNase A and 5 U RNase T1 (Thermo Scientific). The reaction was stopped by extracting the RNA with phenol-chlorophorm. The RNase A/T1-digested sample was phosphorylated with T4 PNK (NEB) and purified with RNA Clean & Concentrator™ kit (Zymo Research). Both the V1 and A/T1 digested samples were randomly fragmented in buffer with pH 9.2 (100 mM Na2CO3, 2 mM EDTA) for 12 min at 95°C. PGCGROWTHCONDITIONS SRR1692169 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli MC4100 strain was cultured at <Temp> 37 °C </Temp> to <Phase> mid-log phase </Phase> ( OD600 ~ 0.4 ) in <Med> LB media </Med> GPL14548-GPL18945 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18945: Illumina Genome Analyzer IIx (Escherichia coli) GSE63817 Secondary structure across the bacterial transcriptome reveals versatile roles in mRNA regulation and function GSM1558082 LB mRNA biological replicate 26495981 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE63817/GSE63817.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli MC4100 strain was cultured at 37°C to mid-log phase (OD600 ~ 0.4) in LB media PGCGROWTHCONDITIONS SRR1692169 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C Total RNA was extracted using TRIzol reagent ( Invitrogen ) and the sample was enriched in mRNA , depleting small RNAs with GeneJET ™ RNA Purification Kit ( Fermentas ) and ribosomal RNA with two cycle of MICROBExpress ™ Bacterial mRNA Enrichment Kit ( Ambion ) . To probe the RNA structure two µg of enriched mRNA were resuspended in 45 µl of DEPC water and denatured for 3 min at 95 °C , refolded at <Temp> 37 °C </Temp> , after addition of 10x RNA-structure buffer with pH 7.0 ( 100 mM Tris , 1 M KCl , 100 mM MgCl2 ) and digested for 1 min at <Temp> 37 °C </Temp> with either 0.05 U RNase V1 ( Life Technologies ) or a combination of 2 µg RNase A and 5 U RNase T1 ( Thermo Scientific ) . The reaction was stopped by extracting the RNA with phenol-chlorophorm . The RNase A/T1-digested sample was phosphorylated with T4 PNK ( NEB ) and purified with RNA Clean & Concentrator ™ kit ( Zymo Research ) . Both the V1 and A/T1 digested samples were randomly fragmented in buffer with pH 9.2 ( 100 mM Na2CO3 , 2 mM EDTA ) for 12 min at 95 °C . GPL14548-GPL18945 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18945: Illumina Genome Analyzer IIx (Escherichia coli) GSE63817 Secondary structure across the bacterial transcriptome reveals versatile roles in mRNA regulation and function GSM1558086 AT1 biological replicate 26495981 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE63817/GSE63817.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Total RNA was extracted using TRIzol reagent (Invitrogen) and the sample was enriched in mRNA, depleting small RNAs with GeneJET™ RNA Purification Kit (Fermentas) and ribosomal RNA with two cycle of MICROBExpress™Bacterial mRNA Enrichment Kit (Ambion). To probe the RNA structure two µg of enriched mRNA were resuspended in 45 µl of DEPC water and denatured for 3 min at 95°C,refolded at 37 °C, after addition of 10x RNA-structure buffer with pH 7.0 (100 mM Tris, 1 M KCl, 100 mM MgCl2) and digested for 1 min at 37 °C with either 0.05 U RNase V1 (Life Technologies) or a combination of 2 µg RNase A and 5 U RNase T1 (Thermo Scientific). The reaction was stopped by extracting the RNA with phenol-chlorophorm. The RNase A/T1-digested sample was phosphorylated with T4 PNK (NEB) and purified with RNA Clean & Concentrator™ kit (Zymo Research). Both the V1 and A/T1 digested samples were randomly fragmented in buffer with pH 9.2 (100 mM Na2CO3, 2 mM EDTA) for 12 min at 95°C. PGCGROWTHCONDITIONS SRR1692173 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli MC4100 strain was cultured at <Temp> 37 °C </Temp> to <Phase> mid-log phase </Phase> ( OD600 ~ 0.4 ) in <Med> LB media </Med> GPL14548-GPL18945 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18945: Illumina Genome Analyzer IIx (Escherichia coli) GSE63817 Secondary structure across the bacterial transcriptome reveals versatile roles in mRNA regulation and function GSM1558086 AT1 biological replicate 26495981 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE63817/GSE63817.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli MC4100 strain was cultured at 37°C to mid-log phase (OD600 ~ 0.4) in LB media PGCGROWTHCONDITIONS SRR1692173 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Med> LB medium , </Med> <Temp> 37 °C </Temp> , airated , constant pH 7.5 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE65244 Temporal gene expression in Escherichia coli GSM1590712 Wt – 60 min http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65244/GSE65244.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt LB medium, 37°C, airated, constant pH 7.5 PGCGROWTHCONDITIONS SRR1771414 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Med> LB medium , </Med> <Temp> 37 °C </Temp> , airated , constant pH 7.5 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE65244 Temporal gene expression in Escherichia coli GSM1590717 Fis – 60 min http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65244/GSE65244.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt LB medium, 37°C, airated, constant pH 7.5 PGCGROWTHCONDITIONS SRR1771419 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Med> LB medium , </Med> <Temp> 37 °C </Temp> , airated , constant pH 7.5 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE65244 Temporal gene expression in Escherichia coli GSM1590718 Fis – 120 min http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65244/GSE65244.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt LB medium, 37°C, airated, constant pH 7.5 PGCGROWTHCONDITIONS SRR1771420 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Med> LB medium , </Med> <Temp> 37 °C </Temp> , airated , constant pH 7.5 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE65244 Temporal gene expression in Escherichia coli GSM1590719 Fis – 180 min http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65244/GSE65244.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt LB medium, 37°C, airated, constant pH 7.5 PGCGROWTHCONDITIONS SRR1771421 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Med> LB medium , </Med> <Temp> 37 °C </Temp> , airated , constant pH 7.5 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE65244 Temporal gene expression in Escherichia coli GSM1590720 Fis – 420 min http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65244/GSE65244.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt LB medium, 37°C, airated, constant pH 7.5 PGCGROWTHCONDITIONS SRR1771422 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Med> LB medium , </Med> <Temp> 37 °C </Temp> , airated , constant pH 7.5 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE65244 Temporal gene expression in Escherichia coli GSM1590721 Hns – 60 min http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65244/GSE65244.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt LB medium, 37°C, airated, constant pH 7.5 PGCGROWTHCONDITIONS SRR1771423 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Med> LB medium , </Med> <Temp> 37 °C </Temp> , airated , constant pH 7.5 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE65244 Temporal gene expression in Escherichia coli GSM1590722 Hns – 120 min http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65244/GSE65244.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt LB medium, 37°C, airated, constant pH 7.5 PGCGROWTHCONDITIONS SRR1771424 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602347 WT glucose 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787590 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602348 WT glucose 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787591 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602349 WT fructose 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787592 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602350 WT fructose 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787593 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602351 WT acetate 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787594 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602352 WT acetate 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787595 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602353 Δcra glucose 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787596 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602354 Δcra glucose 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787597 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602355 Δcra fructose 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787598 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602356 Δcra fructose 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787599 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602357 Δcra acetate 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787600 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602358 Δcra acetate 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787601 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , ΔoxyR , ΔsoxR , and ΔsoxS were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603386 WT PQ 1 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, ΔoxyR, ΔsoxR, and ΔsoxS were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation. PGCGROWTHCONDITIONS SRR1796598 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , ΔoxyR , ΔsoxR , and ΔsoxS were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603387 WT PQ 2 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, ΔoxyR, ΔsoxR, and ΔsoxS were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation. PGCGROWTHCONDITIONS SRR1796599 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , ΔoxyR , ΔsoxR , and ΔsoxS were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603388 ΔoxyR PQ 1 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, ΔoxyR, ΔsoxR, and ΔsoxS were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation. PGCGROWTHCONDITIONS SRR1796600 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , ΔoxyR , ΔsoxR , and ΔsoxS were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603389 ΔoxyR PQ 2 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, ΔoxyR, ΔsoxR, and ΔsoxS were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation. PGCGROWTHCONDITIONS SRR1796601 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , ΔoxyR , ΔsoxR , and ΔsoxS were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603391 ΔsoxR PQ 2 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, ΔoxyR, ΔsoxR, and ΔsoxS were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation. PGCGROWTHCONDITIONS SRR1796603 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , ΔoxyR , ΔsoxR , and ΔsoxS were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603392 ΔsoxS PQ 1 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, ΔoxyR, ΔsoxR, and ΔsoxS were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation. PGCGROWTHCONDITIONS SRR1796604 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , ΔoxyR , ΔsoxR , and ΔsoxS were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603393 ΔsoxS PQ 2 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, ΔoxyR, ΔsoxR, and ΔsoxS were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation. PGCGROWTHCONDITIONS SRR1796605 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623160 WT pH5.5 1 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824557 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623161 WT pH5.5 2 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824558 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623162 ΔgadE pH5.5 1 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824559 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623163 ΔgadE pH5.5 2 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824560 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623164 ΔgadW pH5.5 1 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824561 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623165 ΔgadW pH5.5 2 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824562 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623166 ΔgadX pH5.5 1 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824563 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623167 ΔgadX pH5.5 2 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824564 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900470 Parent LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547467 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900472 cysG KO LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547469 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900474 cysH KO LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547471 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900475 cysH KO LB rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547472 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900476 dcd KO LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547473 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900477 dcd KO LB rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547474 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900478 fadr KO LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547475 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900479 fadr KO LB rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547476 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900480 ppk KO LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547477 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900482 wzc KO LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547479 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900483 wzc KO LB rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547480 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900485 yghD KO LB rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547482 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900486 fepA KO LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547483 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900488 lacA KO LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547485 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900490 Parent M9 rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547487 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900491 Parent M9 rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547488 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900492 dcd KO M9 rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547489 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900493 dcd KO M9 rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547490 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900494 fadr KO M9 rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547491 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900495 fadr KO M9 rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547492 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900496 ppk KO M9 rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547493 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900497 ppk KO M9 rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547494 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900498 wzc KO M9 rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547495 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900499 wzc KO M9 rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547496 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900500 yghD KO M9 rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547497 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900501 yghD KO M9 rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547498 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900502 fepA KO M9 rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547499 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900507 WT rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547504 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900509 mgtA KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547506 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900510 mgtA KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547507 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900513 gabT KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547510 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900514 gabT KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547511 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900515 sdhC KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547512 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900516 sdhC KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547513 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900518 putP KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547515 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900520 putP KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547517 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900521 rfbA KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547518 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900522 rfbA KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547519 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900524 entF KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547521 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900525 entF KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547522 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900526 entF KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547523 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900527 kefB KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547524 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900529 kefB KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547526 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900530 cysA KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547527 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900531 cysA KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547528 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900532 cysA KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547529 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900534 galE KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547531 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900536 mhpD KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547533 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900537 mhpD KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547534 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900538 mhpD KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547535 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900539 fliY KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547536 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900540 fliY KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547537 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900541 fliY KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547538 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900542 lplA KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547539 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900543 lplA KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547540 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900544 lplA KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547541 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900545 khc KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547542 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900546 khc KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547543 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900547 khc KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547544 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900548 ugpC KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547545 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900549 ugpC KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547546 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900550 ugpC KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547547 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900551 trpD KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547548 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900552 trpD KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547549 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900553 trpD KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547550 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900554 aspC KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547551 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900555 aspC KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547552 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900556 aspC KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547553 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All cultures were based on MOPS media with <Supp> 0.2 % glucose </Supp> ( Teknova ) , with full supplement ( Neidhardt et al. , 1974 ) minus methionine . An overnight liquid culture was diluted 400-fold into 200 ml <Med> fresh media </Med> . The culture was kept in a 1 liter flask at <Temp> 37 °C </Temp> with aeration until OD420 reached 0.4 . GPL14548-GPL21433 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE77617 Operon mRNAs are organized into ORF-centric structures that predict translation efficiency GSM2462936 mRNA-seq 37°C in WT with control plasmid 28139975 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE77617/GSE77617.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All cultures were based on MOPS media with 0.2% glucose (Teknova), with full supplement (Neidhardt et al., 1974) minus methionine. An overnight liquid culture was diluted 400-fold into 200 ml fresh media. The culture was kept in a 1 liter flask at 37°C with aeration until OD420 reached 0.4. PGCGROWTHCONDITIONS SRR5186139 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of exponentially growing E. coli were incubated with 750 µL DMS for 2 min at <Temp> 37 °C </Temp> . For kasugamycin ( ksg ) experiments , ksg was added to a final concentration of 10 mg/mL to ΔgcvB cells for 2 min at <Temp> 37 °C </Temp> prior to DMS modification . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) . For in vitro DMS modifications , mRNA was denatured at 95 °C for 2 min , cooled on ice and refolded in 90 µL RNA folding buffer ( 10 mM Tris pH 8.0 , 100 mM NaCl , 6 mM MgCl2 ) at <Temp> 37 °C </Temp> for 30 min then incubated in either .2 % DMS for 1 min ( 95 °C ) or 4 % DMS for 5 min ( <Temp> 37 °C </Temp> ) . GPL14548-GPL21433 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE77617 Operon mRNAs are organized into ORF-centric structures that predict translation efficiency GSM2462936 mRNA-seq 37°C in WT with control plasmid 28139975 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE77617/GSE77617.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of exponentially growing E. coli were incubated with 750 µL DMS for 2 min at 37°C. For kasugamycin (ksg) experiments, ksg was added to a final concentration of 10 mg/mL to ΔgcvB cells for 2 min at 37°C prior to DMS modification. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol). For in vitro DMS modifications, mRNA was denatured at 95 °C for 2 min, cooled on ice and refolded in 90 µL RNA folding buffer (10 mM Tris pH 8.0, 100 mM NaCl, 6 mM MgCl2) at 37°C for 30 min then incubated in either .2% DMS for 1 min (95°C) or 4% DMS for 5 min (37°C). PGCGROWTHCONDITIONS SRR5186139 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All cultures were based on MOPS media with <Supp> 0.2 % glucose </Supp> ( Teknova ) , with full supplement ( Neidhardt et al. , 1974 ) minus methionine . An overnight liquid culture was diluted 400-fold into 200 ml <Med> fresh media </Med> . The culture was kept in a 1 liter flask at <Temp> 37 °C </Temp> with aeration until OD420 reached 0.4 . GPL14548-GPL21433 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE77617 Operon mRNAs are organized into ORF-centric structures that predict translation efficiency GSM2462937 mRNA-seq 37°C in WT with plasmid expressing mini-ORF CUA 28139975 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE77617/GSE77617.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All cultures were based on MOPS media with 0.2% glucose (Teknova), with full supplement (Neidhardt et al., 1974) minus methionine. An overnight liquid culture was diluted 400-fold into 200 ml fresh media. The culture was kept in a 1 liter flask at 37°C with aeration until OD420 reached 0.4. PGCGROWTHCONDITIONS SRR5186140 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of exponentially growing E. coli were incubated with 750 µL DMS for 2 min at <Temp> 37 °C </Temp> . For kasugamycin ( ksg ) experiments , ksg was added to a final concentration of 10 mg/mL to ΔgcvB cells for 2 min at <Temp> 37 °C </Temp> prior to DMS modification . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) . For in vitro DMS modifications , mRNA was denatured at 95 °C for 2 min , cooled on ice and refolded in 90 µL RNA folding buffer ( 10 mM Tris pH 8.0 , 100 mM NaCl , 6 mM MgCl2 ) at <Temp> 37 °C </Temp> for 30 min then incubated in either .2 % DMS for 1 min ( 95 °C ) or 4 % DMS for 5 min ( <Temp> 37 °C </Temp> ) . GPL14548-GPL21433 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE77617 Operon mRNAs are organized into ORF-centric structures that predict translation efficiency GSM2462937 mRNA-seq 37°C in WT with plasmid expressing mini-ORF CUA 28139975 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE77617/GSE77617.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of exponentially growing E. coli were incubated with 750 µL DMS for 2 min at 37°C. For kasugamycin (ksg) experiments, ksg was added to a final concentration of 10 mg/mL to ΔgcvB cells for 2 min at 37°C prior to DMS modification. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol). For in vitro DMS modifications, mRNA was denatured at 95 °C for 2 min, cooled on ice and refolded in 90 µL RNA folding buffer (10 mM Tris pH 8.0, 100 mM NaCl, 6 mM MgCl2) at 37°C for 30 min then incubated in either .2% DMS for 1 min (95°C) or 4% DMS for 5 min (37°C). PGCGROWTHCONDITIONS SRR5186140 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All cultures were based on MOPS media with <Supp> 0.2 % glucose </Supp> ( Teknova ) , with full supplement ( Neidhardt et al. , 1974 ) minus methionine . An overnight liquid culture was diluted 400-fold into 200 ml <Med> fresh media </Med> . The culture was kept in a 1 liter flask at <Temp> 37 °C </Temp> with aeration until OD420 reached 0.4 . GPL14548-GPL21433 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE77617 Operon mRNAs are organized into ORF-centric structures that predict translation efficiency GSM2462938 mRNA-seq 37°C in WT with plasmid expressing mini-ORF CUG 28139975 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE77617/GSE77617.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All cultures were based on MOPS media with 0.2% glucose (Teknova), with full supplement (Neidhardt et al., 1974) minus methionine. An overnight liquid culture was diluted 400-fold into 200 ml fresh media. The culture was kept in a 1 liter flask at 37°C with aeration until OD420 reached 0.4. PGCGROWTHCONDITIONS SRR5186141 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C For in vivo DMS modification , 15 mL of exponentially growing E. coli were incubated with 750 µL DMS for 2 min at <Temp> 37 °C </Temp> . For kasugamycin ( ksg ) experiments , ksg was added to a final concentration of 10 mg/mL to ΔgcvB cells for 2 min at <Temp> 37 °C </Temp> prior to DMS modification . DMS was quenched by adding 30 mL 0 °C stop solution ( 30 % β-mercaptoethanol , 25 % isoamyl alcohol ) . For in vitro DMS modifications , mRNA was denatured at 95 °C for 2 min , cooled on ice and refolded in 90 µL RNA folding buffer ( 10 mM Tris pH 8.0 , 100 mM NaCl , 6 mM MgCl2 ) at <Temp> 37 °C </Temp> for 30 min then incubated in either .2 % DMS for 1 min ( 95 °C ) or 4 % DMS for 5 min ( <Temp> 37 °C </Temp> ) . GPL14548-GPL21433 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE77617 Operon mRNAs are organized into ORF-centric structures that predict translation efficiency GSM2462938 mRNA-seq 37°C in WT with plasmid expressing mini-ORF CUG 28139975 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE77617/GSE77617.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For in vivo DMS modification, 15 mL of exponentially growing E. coli were incubated with 750 µL DMS for 2 min at 37°C. For kasugamycin (ksg) experiments, ksg was added to a final concentration of 10 mg/mL to ΔgcvB cells for 2 min at 37°C prior to DMS modification. DMS was quenched by adding 30 mL 0°C stop solution (30% β-mercaptoethanol, 25% isoamyl alcohol). For in vitro DMS modifications, mRNA was denatured at 95 °C for 2 min, cooled on ice and refolded in 90 µL RNA folding buffer (10 mM Tris pH 8.0, 100 mM NaCl, 6 mM MgCl2) at 37°C for 30 min then incubated in either .2% DMS for 1 min (95°C) or 4% DMS for 5 min (37°C). PGCGROWTHCONDITIONS SRR5186141 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C Total RNA was extracted using the Trizol reagent ( Thermo Fisher Scientific ) and the concentration and purity of the samples were determined using Nano Drop . RNA integrity was monitored using the R6K ScreenTape system on the Agilent 2200 TapeStation . gDNA was removed by Turbo DNase ( Thermo Fisher Scientific ) in the presence of 1U / L RiboLock RNase Inhibitor ( Thermo Fisher Scientific ) for 60 min at <Temp> 37 °C </Temp> . Following organic extraction ( 1x 25:24:1 v/v phenol/chloroform / isoamyalcohol , 1x chloroform ) , RNA was recovered by overnight precipitation with 3 volumes of 30:1 100 % ethanol/3 M sodium acetate ( pH 5.2 ) . Next , the sample ( TEX + ) was depleted of processed RNAs using 1U per 1μg of RNA Terminator 5 ' - Phosphate-Dependent Exonuclease ( TEX , Epicentre ) in the presence of 1U/μL RNase Inhibitor for 60 min at 30 ° C . A control reaction without TEX ( TEX - ) was run in parallel . Following organic extraction , RNA was recovered by overnight precipitation and resuspended in RNase-free water . GPL21475 GPL21475: Illumina HiSeq 2500 (Escherichia coli O104:H4) GSE78041 Differential RNA-Seq (dRNA-seq) of Escherichia coli O104:H4 GSM2065371 TEX-_E. coli O104:H4 27748404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE78041/GSE78041.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Total RNA was extracted using the Trizol reagent (Thermo Fisher Scientific) and the concentration and purity of the samples were determined using Nano Drop. RNA integrity was monitored using the R6K ScreenTape system on the Agilent 2200 TapeStation. gDNA was removed by Turbo DNase (Thermo Fisher Scientific) in the presence of 1U/L RiboLock RNase Inhibitor (Thermo Fisher Scientific) for 60 min at 37°C. Following organic extraction (1x 25:24:1 v/v phenol/chloroform/ isoamyalcohol, 1x chloroform), RNA was recovered by overnight precipitation with 3 volumes of 30:1 100 % ethanol/3 M sodium acetate (pH 5.2). Next, the sample (TEX+) was depleted of processed RNAs using 1U per 1μg of RNA Terminator 5´-Phosphate-Dependent Exonuclease (TEX, Epicentre) in the presence of 1U/μL RNase Inhibitor for 60 min at 30° C. A control reaction without TEX (TEX-) was run in parallel. Following organic extraction, RNA was recovered by overnight precipitation and resuspended in RNase-free water. PGCGROWTHCONDITIONS SRR3176282 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C Total RNA was extracted using the Trizol reagent ( Thermo Fisher Scientific ) and the concentration and purity of the samples were determined using Nano Drop . RNA integrity was monitored using the R6K ScreenTape system on the Agilent 2200 TapeStation . gDNA was removed by Turbo DNase ( Thermo Fisher Scientific ) in the presence of 1U / L RiboLock RNase Inhibitor ( Thermo Fisher Scientific ) for 60 min at <Temp> 37 °C </Temp> . Following organic extraction ( 1x 25:24:1 v/v phenol/chloroform / isoamyalcohol , 1x chloroform ) , RNA was recovered by overnight precipitation with 3 volumes of 30:1 100 % ethanol/3 M sodium acetate ( pH 5.2 ) . Next , the sample ( TEX + ) was depleted of processed RNAs using 1U per 1μg of RNA Terminator 5 ' - Phosphate-Dependent Exonuclease ( TEX , Epicentre ) in the presence of 1U/μL RNase Inhibitor for 60 min at 30 ° C . A control reaction without TEX ( TEX - ) was run in parallel . Following organic extraction , RNA was recovered by overnight precipitation and resuspended in RNase-free water . GPL21475 GPL21475: Illumina HiSeq 2500 (Escherichia coli O104:H4) GSE78041 Differential RNA-Seq (dRNA-seq) of Escherichia coli O104:H4 GSM2065372 TEX+_E. coli O104:H4 27748404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE78041/GSE78041.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Total RNA was extracted using the Trizol reagent (Thermo Fisher Scientific) and the concentration and purity of the samples were determined using Nano Drop. RNA integrity was monitored using the R6K ScreenTape system on the Agilent 2200 TapeStation. gDNA was removed by Turbo DNase (Thermo Fisher Scientific) in the presence of 1U/L RiboLock RNase Inhibitor (Thermo Fisher Scientific) for 60 min at 37°C. Following organic extraction (1x 25:24:1 v/v phenol/chloroform/ isoamyalcohol, 1x chloroform), RNA was recovered by overnight precipitation with 3 volumes of 30:1 100 % ethanol/3 M sodium acetate (pH 5.2). Next, the sample (TEX+) was depleted of processed RNAs using 1U per 1μg of RNA Terminator 5´-Phosphate-Dependent Exonuclease (TEX, Epicentre) in the presence of 1U/μL RNase Inhibitor for 60 min at 30° C. A control reaction without TEX (TEX-) was run in parallel. Following organic extraction, RNA was recovered by overnight precipitation and resuspended in RNase-free water. PGCGROWTHCONDITIONS SRR3176283 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Escherichia coli strains E.coli C ( DSMZ 4860 ) , E. coli Crooks ( DSMZ 1576 ) , E. coli DH5α ( DSMZ 6897 ) E. coli W ( DSMZ 1116 ) , E. coli W3110 ( DSMZ 5911 ) were obtained from DSMZ-German Collection of Microorganism and Cell Cultures ; E. coli BL21 ( DE3 ) was purchased as competent cells from Agilent ( Agilent Technologies Inc. , USA ) , E. coli K-12 MG1655 ( ATCC 700926 ) . All strains were cultured in <Med> M9 minimal medium </Med> ( 1 ) containing Na2HPO4 x 7H2O ( 6.8 g ) , KH2PO4 ( 3 g ) , NaCl ( 0.5 g ) , NH4Cl ( 1 g ) , MgSO4 ( 2 mmol ) , CaCl2 ( 0.1 mmol ) , trace elements , Wolf 's vitamin solution ( 2 ) and glucose ( 2 g L-1 ) . Anoxic <Med> M9 minimal media </Med> with <Supp> glucose </Supp> was obtained by flushing solution with oxygen free nitrogen ( 95 % ) . Overnight cultures from single colonies of each of seven E. coli strains were diluted to a starting optical density ( OD600 ) of 0.01 . Cultures were grown in 250 ml flasks or 300 ml oxygen-free sealed bottles containing 50 ml <Med> glucose-M9 minimal media </Med> in a shaking incubator at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> . GPL14548-GPL15010-GPL21530-GPL21531-GPL21532-GPL21533-GPL21534 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL21530: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. W3110). GPL21531: Illumina HiSeq 2000 (Escherichia coli W). GPL21532: Illumina HiSeq 2000 (Escherichia coli C). GPL21533: Illumina HiSeq 2000 (Escherichia coli BL21). GPL21534: Illumina HiSeq 2000 (Escherichia coli DH5[alpha]) GSE78756 Quantifying variation within the bacterial species E. coli GSM2075722 Crooks_aero 27667363 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE78756/GSE78756.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Escherichia coli strains E.coli C (DSMZ 4860), E. coli Crooks (DSMZ 1576), E. coli DH5α (DSMZ 6897) E. coli W (DSMZ 1116), E. coli W3110 (DSMZ 5911) were obtained from DSMZ-German Collection of Microorganism and Cell Cultures; E. coli BL21 (DE3) was purchased as competent cells from Agilent (Agilent Technologies Inc., USA), E. coli K-12 MG1655 (ATCC 700926). All strains were cultured in M9 minimal medium (1) containing Na2HPO4 x 7H2O (6.8 g), KH2PO4 (3 g), NaCl (0.5 g), NH4Cl (1 g), MgSO4 (2 mmol), CaCl2 (0.1 mmol), trace elements, Wolf’s vitamin solution (2) and glucose (2 g L-1). Anoxic M9 minimal media with glucose was obtained by flushing solution with oxygen free nitrogen (95%). Overnight cultures from single colonies of each of seven E. coli strains were diluted to a starting optical density (OD600) of 0.01. Cultures were grown in 250 ml flasks or 300 ml oxygen-free sealed bottles containing 50 ml glucose-M9 minimal media in a shaking incubator at 37°C and 250 rpm. PGCGROWTHCONDITIONS SRR3194453 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Escherichia coli strains E.coli C ( DSMZ 4860 ) , E. coli Crooks ( DSMZ 1576 ) , E. coli DH5α ( DSMZ 6897 ) E. coli W ( DSMZ 1116 ) , E. coli W3110 ( DSMZ 5911 ) were obtained from DSMZ-German Collection of Microorganism and Cell Cultures ; E. coli BL21 ( DE3 ) was purchased as competent cells from Agilent ( Agilent Technologies Inc. , USA ) , E. coli K-12 MG1655 ( ATCC 700926 ) . All strains were cultured in <Med> M9 minimal medium </Med> ( 1 ) containing Na2HPO4 x 7H2O ( 6.8 g ) , KH2PO4 ( 3 g ) , NaCl ( 0.5 g ) , NH4Cl ( 1 g ) , MgSO4 ( 2 mmol ) , CaCl2 ( 0.1 mmol ) , trace elements , Wolf 's vitamin solution ( 2 ) and glucose ( 2 g L-1 ) . Anoxic <Med> M9 minimal media </Med> with <Supp> glucose </Supp> was obtained by flushing solution with oxygen free nitrogen ( 95 % ) . Overnight cultures from single colonies of each of seven E. coli strains were diluted to a starting optical density ( OD600 ) of 0.01 . Cultures were grown in 250 ml flasks or 300 ml oxygen-free sealed bottles containing 50 ml <Med> glucose-M9 minimal media </Med> in a shaking incubator at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> . GPL14548-GPL15010-GPL21530-GPL21531-GPL21532-GPL21533-GPL21534 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL21530: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. W3110). GPL21531: Illumina HiSeq 2000 (Escherichia coli W). GPL21532: Illumina HiSeq 2000 (Escherichia coli C). GPL21533: Illumina HiSeq 2000 (Escherichia coli BL21). GPL21534: Illumina HiSeq 2000 (Escherichia coli DH5[alpha]) GSE78756 Quantifying variation within the bacterial species E. coli GSM2075723 Crooks_anaero 27667363 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE78756/GSE78756.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Escherichia coli strains E.coli C (DSMZ 4860), E. coli Crooks (DSMZ 1576), E. coli DH5α (DSMZ 6897) E. coli W (DSMZ 1116), E. coli W3110 (DSMZ 5911) were obtained from DSMZ-German Collection of Microorganism and Cell Cultures; E. coli BL21 (DE3) was purchased as competent cells from Agilent (Agilent Technologies Inc., USA), E. coli K-12 MG1655 (ATCC 700926). All strains were cultured in M9 minimal medium (1) containing Na2HPO4 x 7H2O (6.8 g), KH2PO4 (3 g), NaCl (0.5 g), NH4Cl (1 g), MgSO4 (2 mmol), CaCl2 (0.1 mmol), trace elements, Wolf’s vitamin solution (2) and glucose (2 g L-1). Anoxic M9 minimal media with glucose was obtained by flushing solution with oxygen free nitrogen (95%). Overnight cultures from single colonies of each of seven E. coli strains were diluted to a starting optical density (OD600) of 0.01. Cultures were grown in 250 ml flasks or 300 ml oxygen-free sealed bottles containing 50 ml glucose-M9 minimal media in a shaking incubator at 37°C and 250 rpm. PGCGROWTHCONDITIONS SRR3194455SRR3194456 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C The cells were untreated , or treated with ( 100µg/mL Erythromycin or 50 µg/mL Clindamycin in 70 % Ethanol ) . After 10 minutes at <Temp> 37 °C </Temp> , 1 mL samples were subjected to hot phenol-chloroform extraction . Ref : <Gtype> Chuang </Gtype> SE , Daniels DL , and Blattner FR ( 1993 ) Global regulation of gene expression in Escherichia coli . J. Bacteriol . 175:2026-2036 . GPL21726 GPL21726: Ion Torrent Proton (Escherichia coli) GSE80251 Genome-wide transcriptome analyses of E. coli treated with ribosome-targeting antibiotics GSM2122743 Untreated_replicate_1 27645242 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80251/GSE80251.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt The cells were untreated, or treated with (100µg/mL Erythromycin or 50 µg/mL Clindamycin in 70% Ethanol). After 10 minutes at 37°C, 1 mL samples were subjected to hot phenol-chloroform extraction. Ref: Chuang SE, Daniels DL, and Blattner FR (1993) Global regulation of gene expression in Escherichia coli. J. Bacteriol. 175:2026-2036. PGCGROWTHCONDITIONS SRR3379590 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C The cells were untreated , or treated with ( 100µg/mL Erythromycin or 50 µg/mL Clindamycin in 70 % Ethanol ) . After 10 minutes at <Temp> 37 °C </Temp> , 1 mL samples were subjected to hot phenol-chloroform extraction . Ref : <Gtype> Chuang </Gtype> SE , Daniels DL , and Blattner FR ( 1993 ) Global regulation of gene expression in Escherichia coli . J. Bacteriol . 175:2026-2036 . GPL21726 GPL21726: Ion Torrent Proton (Escherichia coli) GSE80251 Genome-wide transcriptome analyses of E. coli treated with ribosome-targeting antibiotics GSM2122744 Untreated_replicate_2 27645242 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80251/GSE80251.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt The cells were untreated, or treated with (100µg/mL Erythromycin or 50 µg/mL Clindamycin in 70% Ethanol). After 10 minutes at 37°C, 1 mL samples were subjected to hot phenol-chloroform extraction. Ref: Chuang SE, Daniels DL, and Blattner FR (1993) Global regulation of gene expression in Escherichia coli. J. Bacteriol. 175:2026-2036. PGCGROWTHCONDITIONS SRR3379591 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C The cells were untreated , or treated with ( 100µg/mL Erythromycin or 50 µg/mL Clindamycin in 70 % Ethanol ) . After 10 minutes at <Temp> 37 °C </Temp> , 1 mL samples were subjected to hot phenol-chloroform extraction . Ref : <Gtype> Chuang </Gtype> SE , Daniels DL , and Blattner FR ( 1993 ) Global regulation of gene expression in Escherichia coli . J. Bacteriol . 175:2026-2036 . GPL21726 GPL21726: Ion Torrent Proton (Escherichia coli) GSE80251 Genome-wide transcriptome analyses of E. coli treated with ribosome-targeting antibiotics GSM2122745 Untreated_replicate_3 27645242 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80251/GSE80251.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt The cells were untreated, or treated with (100µg/mL Erythromycin or 50 µg/mL Clindamycin in 70% Ethanol). After 10 minutes at 37°C, 1 mL samples were subjected to hot phenol-chloroform extraction. Ref: Chuang SE, Daniels DL, and Blattner FR (1993) Global regulation of gene expression in Escherichia coli. J. Bacteriol. 175:2026-2036. PGCGROWTHCONDITIONS SRR3379592 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C The cells were untreated , or treated with ( 100µg/mL Erythromycin or 50 µg/mL Clindamycin in 70 % Ethanol ) . After 10 minutes at <Temp> 37 °C </Temp> , 1 mL samples were subjected to hot phenol-chloroform extraction . Ref : <Gtype> Chuang </Gtype> SE , Daniels DL , and Blattner FR ( 1993 ) Global regulation of gene expression in Escherichia coli . J. Bacteriol . 175:2026-2036 . GPL21726 GPL21726: Ion Torrent Proton (Escherichia coli) GSE80251 Genome-wide transcriptome analyses of E. coli treated with ribosome-targeting antibiotics GSM2122746 Erythromycin_replicate_1 27645242 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80251/GSE80251.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt The cells were untreated, or treated with (100µg/mL Erythromycin or 50 µg/mL Clindamycin in 70% Ethanol). After 10 minutes at 37°C, 1 mL samples were subjected to hot phenol-chloroform extraction. Ref: Chuang SE, Daniels DL, and Blattner FR (1993) Global regulation of gene expression in Escherichia coli. J. Bacteriol. 175:2026-2036. PGCGROWTHCONDITIONS SRR3379593 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C The cells were untreated , or treated with ( 100µg/mL Erythromycin or 50 µg/mL Clindamycin in 70 % Ethanol ) . After 10 minutes at <Temp> 37 °C </Temp> , 1 mL samples were subjected to hot phenol-chloroform extraction . Ref : <Gtype> Chuang </Gtype> SE , Daniels DL , and Blattner FR ( 1993 ) Global regulation of gene expression in Escherichia coli . J. Bacteriol . 175:2026-2036 . GPL21726 GPL21726: Ion Torrent Proton (Escherichia coli) GSE80251 Genome-wide transcriptome analyses of E. coli treated with ribosome-targeting antibiotics GSM2122747 Erythromycin_replicate_2 27645242 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80251/GSE80251.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt The cells were untreated, or treated with (100µg/mL Erythromycin or 50 µg/mL Clindamycin in 70% Ethanol). After 10 minutes at 37°C, 1 mL samples were subjected to hot phenol-chloroform extraction. Ref: Chuang SE, Daniels DL, and Blattner FR (1993) Global regulation of gene expression in Escherichia coli. J. Bacteriol. 175:2026-2036. PGCGROWTHCONDITIONS SRR3379594 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C The cells were untreated , or treated with ( 100µg/mL Erythromycin or 50 µg/mL Clindamycin in 70 % Ethanol ) . After 10 minutes at <Temp> 37 °C </Temp> , 1 mL samples were subjected to hot phenol-chloroform extraction . Ref : <Gtype> Chuang </Gtype> SE , Daniels DL , and Blattner FR ( 1993 ) Global regulation of gene expression in Escherichia coli . J. Bacteriol . 175:2026-2036 . GPL21726 GPL21726: Ion Torrent Proton (Escherichia coli) GSE80251 Genome-wide transcriptome analyses of E. coli treated with ribosome-targeting antibiotics GSM2122748 Erythromycin_replicate_3 27645242 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80251/GSE80251.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt The cells were untreated, or treated with (100µg/mL Erythromycin or 50 µg/mL Clindamycin in 70% Ethanol). After 10 minutes at 37°C, 1 mL samples were subjected to hot phenol-chloroform extraction. Ref: Chuang SE, Daniels DL, and Blattner FR (1993) Global regulation of gene expression in Escherichia coli. J. Bacteriol. 175:2026-2036. PGCGROWTHCONDITIONS SRR3379595 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C The cells were untreated , or treated with ( 100µg/mL Erythromycin or 50 µg/mL Clindamycin in 70 % Ethanol ) . After 10 minutes at <Temp> 37 °C </Temp> , 1 mL samples were subjected to hot phenol-chloroform extraction . Ref : <Gtype> Chuang </Gtype> SE , Daniels DL , and Blattner FR ( 1993 ) Global regulation of gene expression in Escherichia coli . J. Bacteriol . 175:2026-2036 . GPL21726 GPL21726: Ion Torrent Proton (Escherichia coli) GSE80251 Genome-wide transcriptome analyses of E. coli treated with ribosome-targeting antibiotics GSM2122749 Clindamycin_replicate_1 27645242 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80251/GSE80251.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt The cells were untreated, or treated with (100µg/mL Erythromycin or 50 µg/mL Clindamycin in 70% Ethanol). After 10 minutes at 37°C, 1 mL samples were subjected to hot phenol-chloroform extraction. Ref: Chuang SE, Daniels DL, and Blattner FR (1993) Global regulation of gene expression in Escherichia coli. J. Bacteriol. 175:2026-2036. PGCGROWTHCONDITIONS SRR3379596 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C The cells were untreated , or treated with ( 100µg/mL Erythromycin or 50 µg/mL Clindamycin in 70 % Ethanol ) . After 10 minutes at <Temp> 37 °C </Temp> , 1 mL samples were subjected to hot phenol-chloroform extraction . Ref : <Gtype> Chuang </Gtype> SE , Daniels DL , and Blattner FR ( 1993 ) Global regulation of gene expression in Escherichia coli . J. Bacteriol . 175:2026-2036 . GPL21726 GPL21726: Ion Torrent Proton (Escherichia coli) GSE80251 Genome-wide transcriptome analyses of E. coli treated with ribosome-targeting antibiotics GSM2122750 Clindamycin_replicate_2 27645242 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80251/GSE80251.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt The cells were untreated, or treated with (100µg/mL Erythromycin or 50 µg/mL Clindamycin in 70% Ethanol). After 10 minutes at 37°C, 1 mL samples were subjected to hot phenol-chloroform extraction. Ref: Chuang SE, Daniels DL, and Blattner FR (1993) Global regulation of gene expression in Escherichia coli. J. Bacteriol. 175:2026-2036. PGCGROWTHCONDITIONS SRR3379597 MCO000003199 37 °C Temperature True
treatment_protocol 35.7 C The cells were untreated , or treated with ( 100µg/mL Erythromycin or 50 µg/mL Clindamycin in 70 % Ethanol ) . After 10 minutes at <Temp> 37 °C </Temp> , 1 mL samples were subjected to hot phenol-chloroform extraction . Ref : <Gtype> Chuang </Gtype> SE , Daniels DL , and Blattner FR ( 1993 ) Global regulation of gene expression in Escherichia coli . J. Bacteriol . 175:2026-2036 . GPL21726 GPL21726: Ion Torrent Proton (Escherichia coli) GSE80251 Genome-wide transcriptome analyses of E. coli treated with ribosome-targeting antibiotics GSM2122751 Clindamycin_replicate_3 27645242 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80251/GSE80251.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt The cells were untreated, or treated with (100µg/mL Erythromycin or 50 µg/mL Clindamycin in 70% Ethanol). After 10 minutes at 37°C, 1 mL samples were subjected to hot phenol-chloroform extraction. Ref: Chuang SE, Daniels DL, and Blattner FR (1993) Global regulation of gene expression in Escherichia coli. J. Bacteriol. 175:2026-2036. PGCGROWTHCONDITIONS SRR3379598 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic and anaerobic </Air> cultures were grown in 600 mL aliquots of DM25 and incubated at <Temp> 37 °C </Temp> with an orbital shaking of 150 RPM and inoculated with 1/100th volume of overnight culture . Aerobic cultures were grown for 9 h while anaerobic cultures were grown for 16 h. GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE80451 Transcriptome analysis of E. coli REL4536 grown aerobically and anaerobically GSM2127617 Aerobic 1 28103245 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80451/GSE80451.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic and anaerobic cultures were grown in 600 mL aliquots of DM25 and incubated at 37°C with an orbital shaking of 150 RPM and inoculated with 1/100th volume of overnight culture. Aerobic cultures were grown for 9 h while anaerobic cultures were grown for 16 h. PGCGROWTHCONDITIONS SRR3403686 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic and anaerobic </Air> cultures were grown in 600 mL aliquots of DM25 and incubated at <Temp> 37 °C </Temp> with an orbital shaking of 150 RPM and inoculated with 1/100th volume of overnight culture . Aerobic cultures were grown for 9 h while anaerobic cultures were grown for 16 h. GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE80451 Transcriptome analysis of E. coli REL4536 grown aerobically and anaerobically GSM2127618 Aerobic 2 28103245 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80451/GSE80451.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic and anaerobic cultures were grown in 600 mL aliquots of DM25 and incubated at 37°C with an orbital shaking of 150 RPM and inoculated with 1/100th volume of overnight culture. Aerobic cultures were grown for 9 h while anaerobic cultures were grown for 16 h. PGCGROWTHCONDITIONS SRR3403687 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C <Air> Aerobic and anaerobic </Air> cultures were grown in 600 mL aliquots of DM25 and incubated at <Temp> 37 °C </Temp> with an orbital shaking of 150 RPM and inoculated with 1/100th volume of overnight culture . Aerobic cultures were grown for 9 h while anaerobic cultures were grown for 16 h. GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE80451 Transcriptome analysis of E. coli REL4536 grown aerobically and anaerobically GSM2127619 Aerobic 3 28103245 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80451/GSE80451.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic and anaerobic cultures were grown in 600 mL aliquots of DM25 and incubated at 37°C with an orbital shaking of 150 RPM and inoculated with 1/100th volume of overnight culture. Aerobic cultures were grown for 9 h while anaerobic cultures were grown for 16 h. PGCGROWTHCONDITIONS SRR3403688 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C Cell pellets were lysed and RNA collected using Qiagen 's RNeasy Plus Mini Kit with Qiagen Bacteria Protect RNA kit . RNA samples were then treated with DNase ( New England Biolabs ) for <Supp> 30 min </Supp> at <Temp> 37 °C </Temp> . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE81584 Mfd alters global transcription patterns in undamaged Escherichia coli cells GSM2157648 MG1655_1 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE81584/GSE81584.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cell pellets were lysed and RNA collected using Qiagen’s RNeasy Plus Mini Kit with Qiagen Bacteria Protect RNA kit. RNA samples were then treated with DNase (New England Biolabs) for 30 min at 37 °C. PGCGROWTHCONDITIONS SRR3584193SRR3584194SRR3584195 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C Cell pellets were lysed and RNA collected using Qiagen 's RNeasy Plus Mini Kit with Qiagen Bacteria Protect RNA kit . RNA samples were then treated with DNase ( New England Biolabs ) for <Supp> 30 min </Supp> at <Temp> 37 °C </Temp> . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE81584 Mfd alters global transcription patterns in undamaged Escherichia coli cells GSM2157649 MG1655_2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE81584/GSE81584.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cell pellets were lysed and RNA collected using Qiagen’s RNeasy Plus Mini Kit with Qiagen Bacteria Protect RNA kit. RNA samples were then treated with DNase (New England Biolabs) for 30 min at 37 °C. PGCGROWTHCONDITIONS SRR3584196SRR3584197SRR3584198 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C Cell pellets were lysed and RNA collected using Qiagen 's RNeasy Plus Mini Kit with Qiagen Bacteria Protect RNA kit . RNA samples were then treated with DNase ( New England Biolabs ) for <Supp> 30 min </Supp> at <Temp> 37 °C </Temp> . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE81584 Mfd alters global transcription patterns in undamaged Escherichia coli cells GSM2157650 MG1655_3 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE81584/GSE81584.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cell pellets were lysed and RNA collected using Qiagen’s RNeasy Plus Mini Kit with Qiagen Bacteria Protect RNA kit. RNA samples were then treated with DNase (New England Biolabs) for 30 min at 37 °C. PGCGROWTHCONDITIONS SRR3584199SRR3584200 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C Cell pellets were lysed and RNA collected using Qiagen 's RNeasy Plus Mini Kit with Qiagen Bacteria Protect RNA kit . RNA samples were then treated with DNase ( New England Biolabs ) for <Supp> 30 min </Supp> at <Temp> 37 °C </Temp> . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE81584 Mfd alters global transcription patterns in undamaged Escherichia coli cells GSM2157651 mfd_1 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE81584/GSE81584.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cell pellets were lysed and RNA collected using Qiagen’s RNeasy Plus Mini Kit with Qiagen Bacteria Protect RNA kit. RNA samples were then treated with DNase (New England Biolabs) for 30 min at 37 °C. PGCGROWTHCONDITIONS SRR3584201SRR3584202 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C Cell pellets were lysed and RNA collected using Qiagen 's RNeasy Plus Mini Kit with Qiagen Bacteria Protect RNA kit . RNA samples were then treated with DNase ( New England Biolabs ) for <Supp> 30 min </Supp> at <Temp> 37 °C </Temp> . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE81584 Mfd alters global transcription patterns in undamaged Escherichia coli cells GSM2157652 mfd_2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE81584/GSE81584.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cell pellets were lysed and RNA collected using Qiagen’s RNeasy Plus Mini Kit with Qiagen Bacteria Protect RNA kit. RNA samples were then treated with DNase (New England Biolabs) for 30 min at 37 °C. PGCGROWTHCONDITIONS SRR3584203SRR3584204 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C Cell pellets were lysed and RNA collected using Qiagen 's RNeasy Plus Mini Kit with Qiagen Bacteria Protect RNA kit . RNA samples were then treated with DNase ( New England Biolabs ) for <Supp> 30 min </Supp> at <Temp> 37 °C </Temp> . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE81584 Mfd alters global transcription patterns in undamaged Escherichia coli cells GSM2157653 mfd_3 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE81584/GSE81584.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cell pellets were lysed and RNA collected using Qiagen’s RNeasy Plus Mini Kit with Qiagen Bacteria Protect RNA kit. RNA samples were then treated with DNase (New England Biolabs) for 30 min at 37 °C. PGCGROWTHCONDITIONS SRR3584205SRR3584206SRR3584207 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C Cell pellets were lysed and RNA collected using Qiagen 's RNeasy Plus Mini Kit with Qiagen Bacteria Protect RNA kit . RNA samples were then treated with DNase ( New England Biolabs ) for <Supp> 30 min </Supp> at <Temp> 37 °C </Temp> . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE81584 Mfd alters global transcription patterns in undamaged Escherichia coli cells GSM2157654 MG1655_vector_1 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE81584/GSE81584.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cell pellets were lysed and RNA collected using Qiagen’s RNeasy Plus Mini Kit with Qiagen Bacteria Protect RNA kit. RNA samples were then treated with DNase (New England Biolabs) for 30 min at 37 °C. PGCGROWTHCONDITIONS SRR3584208SRR3584209 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C Cell pellets were lysed and RNA collected using Qiagen 's RNeasy Plus Mini Kit with Qiagen Bacteria Protect RNA kit . RNA samples were then treated with DNase ( New England Biolabs ) for <Supp> 30 min </Supp> at <Temp> 37 °C </Temp> . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE81584 Mfd alters global transcription patterns in undamaged Escherichia coli cells GSM2157655 MG1655_vector_2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE81584/GSE81584.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cell pellets were lysed and RNA collected using Qiagen’s RNeasy Plus Mini Kit with Qiagen Bacteria Protect RNA kit. RNA samples were then treated with DNase (New England Biolabs) for 30 min at 37 °C. PGCGROWTHCONDITIONS SRR3584210SRR3584211 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C Cell pellets were lysed and RNA collected using Qiagen 's RNeasy Plus Mini Kit with Qiagen Bacteria Protect RNA kit . RNA samples were then treated with DNase ( New England Biolabs ) for <Supp> 30 min </Supp> at <Temp> 37 °C </Temp> . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE81584 Mfd alters global transcription patterns in undamaged Escherichia coli cells GSM2157656 MFD++_1 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE81584/GSE81584.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cell pellets were lysed and RNA collected using Qiagen’s RNeasy Plus Mini Kit with Qiagen Bacteria Protect RNA kit. RNA samples were then treated with DNase (New England Biolabs) for 30 min at 37 °C. PGCGROWTHCONDITIONS SRR3584212SRR3584213SRR3584214 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C Cell pellets were lysed and RNA collected using Qiagen 's RNeasy Plus Mini Kit with Qiagen Bacteria Protect RNA kit . RNA samples were then treated with DNase ( New England Biolabs ) for <Supp> 30 min </Supp> at <Temp> 37 °C </Temp> . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE81584 Mfd alters global transcription patterns in undamaged Escherichia coli cells GSM2157657 MFD++_2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE81584/GSE81584.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cell pellets were lysed and RNA collected using Qiagen’s RNeasy Plus Mini Kit with Qiagen Bacteria Protect RNA kit. RNA samples were then treated with DNase (New England Biolabs) for 30 min at 37 °C. PGCGROWTHCONDITIONS SRR3584215SRR3584216SRR3584217 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C RNA was extracted from two biological replicates ( 30 mL ) of each bacterial culture isolated at each time point . The 30 mL culture was aliquoted into three 15 mL tubes containing 5 mL of ice-cold EtOH/Phenol stop solution ( 5 % water-saturated phenol pH 4.2 [ Invitrogen ] in 100 % ethanol ) . Cells were collected by centrifugation ( 4,000 rpm , 5 min , 4 °C ) and the pellet suspended in 800 µL TE pH 8.0 supplemented with 0.5 mg/mL lysozyme . SDS ( 80 µL at 10 % ( w/v ) ) was added , samples mixed by inversion , and incubated ( 65 °C , 2 min ) , before the addition of <Supp> 88 µL 1 M </Supp> NaOAc ( pH 5.2 ) . Each sample was added to an equal volume ( 1mL ) of water-saturated phenol ( pH 4.2 , 65 °C ) and incubated ( 65 °C , 6 min ) . The solution was separated into layers by centrifugation ( 14,000 rpm , 10 min , 4 °C ) and the upper aqueous RNA containing layer removed and transferred into a fresh tube . RNA was cleaned by three extractions with phenol : <Gtype> CHCl3 : IAA </Gtype> ( 25:24:1 , pH 8.0 ) before being precipitated ( -80 °C , O/N ) following the addition of 1/10 volume of 3M NaOAc ( pH 5.2 ) and 2 volumes of ice cold 100 % EtOH . RNA was pelleted ( 14,000 rpm , 25 min , 4 °C ) , the supernatant removed and the pellet washed with 1 mL 75 % cold EtOH ( made with DEPC-treated water ) . RNA was pelleted ( 14,000 rpm , 5 min , 4 °C ) and air dried before suspension in 80 µL RNAse free water ( Invitrogen ) and treatment with TURBO DNAse ( Ambion ) according to the manufacturer 's instructions . Briefly , 0.1 V of 10 X Turbo DNAse buffer ( Ambion ) was added to the RNA solution . 1 µL of TURBO DNAse was added to the solution , which was then incubated ( 30 min , <Temp> 37 °C </Temp> ) . Following incubation , 0.1 V of DNase Inactivation Reagent ( Ambion ) was added and then incubated ( 5 min , RT ) with occasional mixing . RNA was isolated and transferred into a fresh microfuge tube . RNA quality tested using the the Agilent RNA 6000 Nano Kit ( Agilent Technologies ) and quality analysed using the Agilent 2100 Bioanalyzer ( Agilent Technologies ) . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE87071 The Escherichia coli nucleoid is shaped around replication and transcription [RNA-Seq] GSM2321583 10J.0 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE87071/GSE87071.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt RNA was extracted from two biological replicates (30 mL) of each bacterial culture isolated at each time point. The 30 mL culture was aliquoted into three 15 mL tubes containing 5 mL of ice-cold EtOH/Phenol stop solution (5% water-saturated phenol pH 4.2 [Invitrogen] in 100% ethanol). Cells were collected by centrifugation (4,000 rpm, 5 min, 4°C) and the pellet suspended in 800 µL TE pH 8.0 supplemented with 0.5 mg/mL lysozyme. SDS (80 µL at 10% (w/v)) was added, samples mixed by inversion, and incubated (65°C, 2 min), before the addition of 88 µL 1 M NaOAc (pH 5.2). Each sample was added to an equal volume (1mL) of water-saturated phenol (pH 4.2, 65°C) and incubated (65°C, 6 min). The solution was separated into layers by centrifugation (14,000 rpm, 10 min, 4°C) and the upper aqueous RNA containing layer removed and transferred into a fresh tube. RNA was cleaned by three extractions with phenol:CHCl3:IAA (25:24:1, pH 8.0) before being precipitated (-80°C, O/N) following the addition of 1/10 volume of 3M NaOAc (pH 5.2) and 2 volumes of ice cold 100% EtOH. RNA was pelleted (14,000 rpm, 25 min, 4°C), the supernatant removed and the pellet washed with 1 mL 75% cold EtOH (made with DEPC-treated water). RNA was pelleted (14,000 rpm, 5 min, 4°C) and air dried before suspension in 80 µL RNAse free water (Invitrogen) and treatment with TURBO DNAse (Ambion) according to the manufacturer’s instructions. Briefly, 0.1 V of 10 X Turbo DNAse buffer (Ambion) was added to the RNA solution. 1 µL of TURBO DNAse was added to the solution, which was then incubated (30 min, 37 °C). Following incubation, 0.1 V of DNase Inactivation Reagent (Ambion) was added and then incubated (5 min, RT) with occasional mixing. RNA was isolated and transferred into a fresh microfuge tube. RNA quality tested using the the Agilent RNA 6000 Nano Kit (Agilent Technologies) and quality analysed using the Agilent 2100 Bioanalyzer (Agilent Technologies). PGCGROWTHCONDITIONS SRR4255368 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Synchronization was achieved by passaging cells through one or two rounds of stationary phase according to Cutler and Evans 1966 . Briefly : <Gtype> Individual </Gtype> colonies ( LB plate , <Temp> 37 °C </Temp> , 24 h ) were inoculated into M9 media and grown O/N ( <Temp> 37 °C </Temp> , 200 rpm ) . M9 media was inoculated to a final OD600 = 0.25 ( early-logarithmic phase ) . Cultures were grown ( <Temp> 37 °C </Temp> , 200 rpm ) and maintained in stationary phase ( OD600 1.8 ) for <Supp> approximately 2 hours </Supp> . An appropriate amount of each culture was used to inoculate pre-warmed ( <Temp> 37 °C </Temp> ) M9 media to a final OD600 = 0.25 ( approximate 7-fold dilution ) . Released cultures were grown ( <Temp> 37 °C </Temp> , 200 rpm ) and harvested ( time = 0 , 1 hr , or 2 hr ) for GCC and RNA isolation . Samples ( 1 mL ) were also taken for FACS analysis and fluorescence microscopy ( t = 0 , 1 hr , and 2 hr ) . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE87071 The Escherichia coli nucleoid is shaped around replication and transcription [RNA-Seq] GSM2321583 10J.0 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE87071/GSE87071.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Synchronization was achieved by passaging cells through one or two rounds of stationary phase according to Cutler and Evans 1966. Briefly: Individual colonies (LB plate, 37°C, 24 h) were inoculated into M9 media and grown O/N (37°C, 200 rpm). M9 media was inoculated to a final OD600 = 0.25 (early-logarithmic phase). Cultures were grown (37°C, 200 rpm) and maintained in stationary phase (OD600 1.8) for approximately 2 hours. An appropriate amount of each culture was used to inoculate pre-warmed (37°C) M9 media to a final OD600 = 0.25 (approximate 7-fold dilution). Released cultures were grown (37°C, 200 rpm) and harvested (time = 0, 1 hr, or 2 hr) for GCC and RNA isolation. Samples (1 mL) were also taken for FACS analysis and fluorescence microscopy (t=0, 1 hr, and 2 hr). PGCGROWTHCONDITIONS SRR4255368 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C RNA was extracted from two biological replicates ( 30 mL ) of each bacterial culture isolated at each time point . The 30 mL culture was aliquoted into three 15 mL tubes containing 5 mL of ice-cold EtOH/Phenol stop solution ( 5 % water-saturated phenol pH 4.2 [ Invitrogen ] in 100 % ethanol ) . Cells were collected by centrifugation ( 4,000 rpm , 5 min , 4 °C ) and the pellet suspended in 800 µL TE pH 8.0 supplemented with 0.5 mg/mL lysozyme . SDS ( 80 µL at 10 % ( w/v ) ) was added , samples mixed by inversion , and incubated ( 65 °C , 2 min ) , before the addition of <Supp> 88 µL 1 M </Supp> NaOAc ( pH 5.2 ) . Each sample was added to an equal volume ( 1mL ) of water-saturated phenol ( pH 4.2 , 65 °C ) and incubated ( 65 °C , 6 min ) . The solution was separated into layers by centrifugation ( 14,000 rpm , 10 min , 4 °C ) and the upper aqueous RNA containing layer removed and transferred into a fresh tube . RNA was cleaned by three extractions with phenol : <Gtype> CHCl3 : IAA </Gtype> ( 25:24:1 , pH 8.0 ) before being precipitated ( -80 °C , O/N ) following the addition of 1/10 volume of 3M NaOAc ( pH 5.2 ) and 2 volumes of ice cold 100 % EtOH . RNA was pelleted ( 14,000 rpm , 25 min , 4 °C ) , the supernatant removed and the pellet washed with 1 mL 75 % cold EtOH ( made with DEPC-treated water ) . RNA was pelleted ( 14,000 rpm , 5 min , 4 °C ) and air dried before suspension in 80 µL RNAse free water ( Invitrogen ) and treatment with TURBO DNAse ( Ambion ) according to the manufacturer 's instructions . Briefly , 0.1 V of 10 X Turbo DNAse buffer ( Ambion ) was added to the RNA solution . 1 µL of TURBO DNAse was added to the solution , which was then incubated ( 30 min , <Temp> 37 °C </Temp> ) . Following incubation , 0.1 V of DNase Inactivation Reagent ( Ambion ) was added and then incubated ( 5 min , RT ) with occasional mixing . RNA was isolated and transferred into a fresh microfuge tube . RNA quality tested using the the Agilent RNA 6000 Nano Kit ( Agilent Technologies ) and quality analysed using the Agilent 2100 Bioanalyzer ( Agilent Technologies ) . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE87071 The Escherichia coli nucleoid is shaped around replication and transcription [RNA-Seq] GSM2321584 11K.60 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE87071/GSE87071.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt RNA was extracted from two biological replicates (30 mL) of each bacterial culture isolated at each time point. The 30 mL culture was aliquoted into three 15 mL tubes containing 5 mL of ice-cold EtOH/Phenol stop solution (5% water-saturated phenol pH 4.2 [Invitrogen] in 100% ethanol). Cells were collected by centrifugation (4,000 rpm, 5 min, 4°C) and the pellet suspended in 800 µL TE pH 8.0 supplemented with 0.5 mg/mL lysozyme. SDS (80 µL at 10% (w/v)) was added, samples mixed by inversion, and incubated (65°C, 2 min), before the addition of 88 µL 1 M NaOAc (pH 5.2). Each sample was added to an equal volume (1mL) of water-saturated phenol (pH 4.2, 65°C) and incubated (65°C, 6 min). The solution was separated into layers by centrifugation (14,000 rpm, 10 min, 4°C) and the upper aqueous RNA containing layer removed and transferred into a fresh tube. RNA was cleaned by three extractions with phenol:CHCl3:IAA (25:24:1, pH 8.0) before being precipitated (-80°C, O/N) following the addition of 1/10 volume of 3M NaOAc (pH 5.2) and 2 volumes of ice cold 100% EtOH. RNA was pelleted (14,000 rpm, 25 min, 4°C), the supernatant removed and the pellet washed with 1 mL 75% cold EtOH (made with DEPC-treated water). RNA was pelleted (14,000 rpm, 5 min, 4°C) and air dried before suspension in 80 µL RNAse free water (Invitrogen) and treatment with TURBO DNAse (Ambion) according to the manufacturer’s instructions. Briefly, 0.1 V of 10 X Turbo DNAse buffer (Ambion) was added to the RNA solution. 1 µL of TURBO DNAse was added to the solution, which was then incubated (30 min, 37 °C). Following incubation, 0.1 V of DNase Inactivation Reagent (Ambion) was added and then incubated (5 min, RT) with occasional mixing. RNA was isolated and transferred into a fresh microfuge tube. RNA quality tested using the the Agilent RNA 6000 Nano Kit (Agilent Technologies) and quality analysed using the Agilent 2100 Bioanalyzer (Agilent Technologies). PGCGROWTHCONDITIONS SRR4255369 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Synchronization was achieved by passaging cells through one or two rounds of stationary phase according to Cutler and Evans 1966 . Briefly : <Gtype> Individual </Gtype> colonies ( LB plate , <Temp> 37 °C </Temp> , 24 h ) were inoculated into M9 media and grown O/N ( <Temp> 37 °C </Temp> , 200 rpm ) . M9 media was inoculated to a final OD600 = 0.25 ( early-logarithmic phase ) . Cultures were grown ( <Temp> 37 °C </Temp> , 200 rpm ) and maintained in stationary phase ( OD600 1.8 ) for <Supp> approximately 2 hours </Supp> . An appropriate amount of each culture was used to inoculate pre-warmed ( <Temp> 37 °C </Temp> ) M9 media to a final OD600 = 0.25 ( approximate 7-fold dilution ) . Released cultures were grown ( <Temp> 37 °C </Temp> , 200 rpm ) and harvested ( time = 0 , 1 hr , or 2 hr ) for GCC and RNA isolation . Samples ( 1 mL ) were also taken for FACS analysis and fluorescence microscopy ( t = 0 , 1 hr , and 2 hr ) . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE87071 The Escherichia coli nucleoid is shaped around replication and transcription [RNA-Seq] GSM2321584 11K.60 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE87071/GSE87071.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Synchronization was achieved by passaging cells through one or two rounds of stationary phase according to Cutler and Evans 1966. Briefly: Individual colonies (LB plate, 37°C, 24 h) were inoculated into M9 media and grown O/N (37°C, 200 rpm). M9 media was inoculated to a final OD600 = 0.25 (early-logarithmic phase). Cultures were grown (37°C, 200 rpm) and maintained in stationary phase (OD600 1.8) for approximately 2 hours. An appropriate amount of each culture was used to inoculate pre-warmed (37°C) M9 media to a final OD600 = 0.25 (approximate 7-fold dilution). Released cultures were grown (37°C, 200 rpm) and harvested (time = 0, 1 hr, or 2 hr) for GCC and RNA isolation. Samples (1 mL) were also taken for FACS analysis and fluorescence microscopy (t=0, 1 hr, and 2 hr). PGCGROWTHCONDITIONS SRR4255369 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C RNA was extracted from two biological replicates ( 30 mL ) of each bacterial culture isolated at each time point . The 30 mL culture was aliquoted into three 15 mL tubes containing 5 mL of ice-cold EtOH/Phenol stop solution ( 5 % water-saturated phenol pH 4.2 [ Invitrogen ] in 100 % ethanol ) . Cells were collected by centrifugation ( 4,000 rpm , 5 min , 4 °C ) and the pellet suspended in 800 µL TE pH 8.0 supplemented with 0.5 mg/mL lysozyme . SDS ( 80 µL at 10 % ( w/v ) ) was added , samples mixed by inversion , and incubated ( 65 °C , 2 min ) , before the addition of <Supp> 88 µL 1 M </Supp> NaOAc ( pH 5.2 ) . Each sample was added to an equal volume ( 1mL ) of water-saturated phenol ( pH 4.2 , 65 °C ) and incubated ( 65 °C , 6 min ) . The solution was separated into layers by centrifugation ( 14,000 rpm , 10 min , 4 °C ) and the upper aqueous RNA containing layer removed and transferred into a fresh tube . RNA was cleaned by three extractions with phenol : <Gtype> CHCl3 : IAA </Gtype> ( 25:24:1 , pH 8.0 ) before being precipitated ( -80 °C , O/N ) following the addition of 1/10 volume of 3M NaOAc ( pH 5.2 ) and 2 volumes of ice cold 100 % EtOH . RNA was pelleted ( 14,000 rpm , 25 min , 4 °C ) , the supernatant removed and the pellet washed with 1 mL 75 % cold EtOH ( made with DEPC-treated water ) . RNA was pelleted ( 14,000 rpm , 5 min , 4 °C ) and air dried before suspension in 80 µL RNAse free water ( Invitrogen ) and treatment with TURBO DNAse ( Ambion ) according to the manufacturer 's instructions . Briefly , 0.1 V of 10 X Turbo DNAse buffer ( Ambion ) was added to the RNA solution . 1 µL of TURBO DNAse was added to the solution , which was then incubated ( 30 min , <Temp> 37 °C </Temp> ) . Following incubation , 0.1 V of DNase Inactivation Reagent ( Ambion ) was added and then incubated ( 5 min , RT ) with occasional mixing . RNA was isolated and transferred into a fresh microfuge tube . RNA quality tested using the the Agilent RNA 6000 Nano Kit ( Agilent Technologies ) and quality analysed using the Agilent 2100 Bioanalyzer ( Agilent Technologies ) . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE87071 The Escherichia coli nucleoid is shaped around replication and transcription [RNA-Seq] GSM2321585 12L.120 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE87071/GSE87071.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt RNA was extracted from two biological replicates (30 mL) of each bacterial culture isolated at each time point. The 30 mL culture was aliquoted into three 15 mL tubes containing 5 mL of ice-cold EtOH/Phenol stop solution (5% water-saturated phenol pH 4.2 [Invitrogen] in 100% ethanol). Cells were collected by centrifugation (4,000 rpm, 5 min, 4°C) and the pellet suspended in 800 µL TE pH 8.0 supplemented with 0.5 mg/mL lysozyme. SDS (80 µL at 10% (w/v)) was added, samples mixed by inversion, and incubated (65°C, 2 min), before the addition of 88 µL 1 M NaOAc (pH 5.2). Each sample was added to an equal volume (1mL) of water-saturated phenol (pH 4.2, 65°C) and incubated (65°C, 6 min). The solution was separated into layers by centrifugation (14,000 rpm, 10 min, 4°C) and the upper aqueous RNA containing layer removed and transferred into a fresh tube. RNA was cleaned by three extractions with phenol:CHCl3:IAA (25:24:1, pH 8.0) before being precipitated (-80°C, O/N) following the addition of 1/10 volume of 3M NaOAc (pH 5.2) and 2 volumes of ice cold 100% EtOH. RNA was pelleted (14,000 rpm, 25 min, 4°C), the supernatant removed and the pellet washed with 1 mL 75% cold EtOH (made with DEPC-treated water). RNA was pelleted (14,000 rpm, 5 min, 4°C) and air dried before suspension in 80 µL RNAse free water (Invitrogen) and treatment with TURBO DNAse (Ambion) according to the manufacturer’s instructions. Briefly, 0.1 V of 10 X Turbo DNAse buffer (Ambion) was added to the RNA solution. 1 µL of TURBO DNAse was added to the solution, which was then incubated (30 min, 37 °C). Following incubation, 0.1 V of DNase Inactivation Reagent (Ambion) was added and then incubated (5 min, RT) with occasional mixing. RNA was isolated and transferred into a fresh microfuge tube. RNA quality tested using the the Agilent RNA 6000 Nano Kit (Agilent Technologies) and quality analysed using the Agilent 2100 Bioanalyzer (Agilent Technologies). PGCGROWTHCONDITIONS SRR4255370 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Synchronization was achieved by passaging cells through one or two rounds of stationary phase according to Cutler and Evans 1966 . Briefly : <Gtype> Individual </Gtype> colonies ( LB plate , <Temp> 37 °C </Temp> , 24 h ) were inoculated into M9 media and grown O/N ( <Temp> 37 °C </Temp> , 200 rpm ) . M9 media was inoculated to a final OD600 = 0.25 ( early-logarithmic phase ) . Cultures were grown ( <Temp> 37 °C </Temp> , 200 rpm ) and maintained in stationary phase ( OD600 1.8 ) for <Supp> approximately 2 hours </Supp> . An appropriate amount of each culture was used to inoculate pre-warmed ( <Temp> 37 °C </Temp> ) M9 media to a final OD600 = 0.25 ( approximate 7-fold dilution ) . Released cultures were grown ( <Temp> 37 °C </Temp> , 200 rpm ) and harvested ( time = 0 , 1 hr , or 2 hr ) for GCC and RNA isolation . Samples ( 1 mL ) were also taken for FACS analysis and fluorescence microscopy ( t = 0 , 1 hr , and 2 hr ) . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE87071 The Escherichia coli nucleoid is shaped around replication and transcription [RNA-Seq] GSM2321585 12L.120 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE87071/GSE87071.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Synchronization was achieved by passaging cells through one or two rounds of stationary phase according to Cutler and Evans 1966. Briefly: Individual colonies (LB plate, 37°C, 24 h) were inoculated into M9 media and grown O/N (37°C, 200 rpm). M9 media was inoculated to a final OD600 = 0.25 (early-logarithmic phase). Cultures were grown (37°C, 200 rpm) and maintained in stationary phase (OD600 1.8) for approximately 2 hours. An appropriate amount of each culture was used to inoculate pre-warmed (37°C) M9 media to a final OD600 = 0.25 (approximate 7-fold dilution). Released cultures were grown (37°C, 200 rpm) and harvested (time = 0, 1 hr, or 2 hr) for GCC and RNA isolation. Samples (1 mL) were also taken for FACS analysis and fluorescence microscopy (t=0, 1 hr, and 2 hr). PGCGROWTHCONDITIONS SRR4255370 MCO000003199 37 °C Temperature True
extract_protocol 35.7 C RNA was extracted from two biological replicates ( 30 mL ) of each bacterial culture isolated at each time point . The 30 mL culture was aliquoted into three 15 mL tubes containing 5 mL of ice-cold EtOH/Phenol stop solution ( 5 % water-saturated phenol pH 4.2 [ Invitrogen ] in 100 % ethanol ) . Cells were collected by centrifugation ( 4,000 rpm , 5 min , 4 °C ) and the pellet suspended in 800 µL TE pH 8.0 supplemented with 0.5 mg/mL lysozyme . SDS ( 80 µL at 10 % ( w/v ) ) was added , samples mixed by inversion , and incubated ( 65 °C , 2 min ) , before the addition of <Supp> 88 µL 1 M </Supp> NaOAc ( pH 5.2 ) . Each sample was added to an equal volume ( 1mL ) of water-saturated phenol ( pH 4.2 , 65 °C ) and incubated ( 65 °C , 6 min ) . The solution was separated into layers by centrifugation ( 14,000 rpm , 10 min , 4 °C ) and the upper aqueous RNA containing layer removed and transferred into a fresh tube . RNA was cleaned by three extractions with phenol : <Gtype> CHCl3 : IAA </Gtype> ( 25:24:1 , pH 8.0 ) before being precipitated ( -80 °C , O/N ) following the addition of 1/10 volume of 3M NaOAc ( pH 5.2 ) and 2 volumes of ice cold 100 % EtOH . RNA was pelleted ( 14,000 rpm , 25 min , 4 °C ) , the supernatant removed and the pellet washed with 1 mL 75 % cold EtOH ( made with DEPC-treated water ) . RNA was pelleted ( 14,000 rpm , 5 min , 4 °C ) and air dried before suspension in 80 µL RNAse free water ( Invitrogen ) and treatment with TURBO DNAse ( Ambion ) according to the manufacturer 's instructions . Briefly , 0.1 V of 10 X Turbo DNAse buffer ( Ambion ) was added to the RNA solution . 1 µL of TURBO DNAse was added to the solution , which was then incubated ( 30 min , <Temp> 37 °C </Temp> ) . Following incubation , 0.1 V of DNase Inactivation Reagent ( Ambion ) was added and then incubated ( 5 min , RT ) with occasional mixing . RNA was isolated and transferred into a fresh microfuge tube . RNA quality tested using the the Agilent RNA 6000 Nano Kit ( Agilent Technologies ) and quality analysed using the Agilent 2100 Bioanalyzer ( Agilent Technologies ) . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE87071 The Escherichia coli nucleoid is shaped around replication and transcription [RNA-Seq] GSM2321586 7G.0 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE87071/GSE87071.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt RNA was extracted from two biological replicates (30 mL) of each bacterial culture isolated at each time point. The 30 mL culture was aliquoted into three 15 mL tubes containing 5 mL of ice-cold EtOH/Phenol stop solution (5% water-saturated phenol pH 4.2 [Invitrogen] in 100% ethanol). Cells were collected by centrifugation (4,000 rpm, 5 min, 4°C) and the pellet suspended in 800 µL TE pH 8.0 supplemented with 0.5 mg/mL lysozyme. SDS (80 µL at 10% (w/v)) was added, samples mixed by inversion, and incubated (65°C, 2 min), before the addition of 88 µL 1 M NaOAc (pH 5.2). Each sample was added to an equal volume (1mL) of water-saturated phenol (pH 4.2, 65°C) and incubated (65°C, 6 min). The solution was separated into layers by centrifugation (14,000 rpm, 10 min, 4°C) and the upper aqueous RNA containing layer removed and transferred into a fresh tube. RNA was cleaned by three extractions with phenol:CHCl3:IAA (25:24:1, pH 8.0) before being precipitated (-80°C, O/N) following the addition of 1/10 volume of 3M NaOAc (pH 5.2) and 2 volumes of ice cold 100% EtOH. RNA was pelleted (14,000 rpm, 25 min, 4°C), the supernatant removed and the pellet washed with 1 mL 75% cold EtOH (made with DEPC-treated water). RNA was pelleted (14,000 rpm, 5 min, 4°C) and air dried before suspension in 80 µL RNAse free water (Invitrogen) and treatment with TURBO DNAse (Ambion) according to the manufacturer’s instructions. Briefly, 0.1 V of 10 X Turbo DNAse buffer (Ambion) was added to the RNA solution. 1 µL of TURBO DNAse was added to the solution, which was then incubated (30 min, 37 °C). Following incubation, 0.1 V of DNase Inactivation Reagent (Ambion) was added and then incubated (5 min, RT) with occasional mixing. RNA was isolated and transferred into a fresh microfuge tube. RNA quality tested using the the Agilent RNA 6000 Nano Kit (Agilent Technologies) and quality analysed using the Agilent 2100 Bioanalyzer (Agilent Technologies). PGCGROWTHCONDITIONS SRR4255371 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Synchronization was achieved by passaging cells through one or two rounds of stationary phase according to Cutler and Evans 1966 . Briefly : <Gtype> Individual </Gtype> colonies ( LB plate , <Temp> 37 °C </Temp> , 24 h ) were inoculated into M9 media and grown O/N ( <Temp> 37 °C </Temp> , 200 rpm ) . M9 media was inoculated to a final OD600 = 0.25 ( early-logarithmic phase ) . Cultures were grown ( <Temp> 37 °C </Temp> , 200 rpm ) and maintained in stationary phase ( OD600 1.8 ) for <Supp> approximately 2 hours </Supp> . An appropriate amount of each culture was used to inoculate pre-warmed ( <Temp> 37 °C </Temp> ) M9 media to a final OD600 = 0.25 ( approximate 7-fold dilution ) . Released cultures were grown ( <Temp> 37 °C </Temp> , 200 rpm ) and harvested ( time = 0 , 1 hr , or 2 hr ) for GCC and RNA isolation . Samples ( 1 mL ) were also taken for FACS analysis and fluorescence microscopy ( t = 0 , 1 hr , and 2 hr ) . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE87071 The Escherichia coli nucleoid is shaped around replication and transcription [RNA-Seq] GSM2321586 7G.0 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE87071/GSE87071.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Synchronization was achieved by passaging cells through one or two rounds of stationary phase according to Cutler and Evans 1966. Briefly: Individual colonies (LB plate, 37°C, 24 h) were inoculated into M9 media and grown O/N (37°C, 200 rpm). M9 media was inoculated to a final OD600 = 0.25 (early-logarithmic phase). Cultures were grown (37°C, 200 rpm) and maintained in stationary phase (OD600 1.8) for approximately 2 hours. An appropriate amount of each culture was used to inoculate pre-warmed (37°C) M9 media to a final OD600 = 0.25 (approximate 7-fold dilution). Released cultures were grown (37°C, 200 rpm) and harvested (time = 0, 1 hr, or 2 hr) for GCC and RNA isolation. Samples (1 mL) were also taken for FACS analysis and fluorescence microscopy (t=0, 1 hr, and 2 hr). PGCGROWTHCONDITIONS SRR4255371 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All strains were grown shaking at <Temp> 37 °C </Temp> in 200mL cultures of <Med> MOPS complete-glucose liquid media </Med> ( unless otherwise annotated ) in 1L flasks and cells were harvested at OD ( 420nm ) between 0.4 - 0.6 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE88725 Global analysis of translation termination in E. coli using release factor manipulations GSM2344783 WT_exp1_mRNA 28301469 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88725/GSE88725.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All strains were grown shaking at 37°C in 200mL cultures of MOPS complete-glucose liquid media (unless otherwise annotated) in 1L flasks and cells were harvested at OD(420nm) between 0.4 - 0.6 PGCGROWTHCONDITIONS SRR4421263 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All strains were grown shaking at <Temp> 37 °C </Temp> in 200mL cultures of <Med> MOPS complete-glucose liquid media </Med> ( unless otherwise annotated ) in 1L flasks and cells were harvested at OD ( 420nm ) between 0.4 - 0.6 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE88725 Global analysis of translation termination in E. coli using release factor manipulations GSM2344784 WT_exp2_mRNA 28301469 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88725/GSE88725.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All strains were grown shaking at 37°C in 200mL cultures of MOPS complete-glucose liquid media (unless otherwise annotated) in 1L flasks and cells were harvested at OD(420nm) between 0.4 - 0.6 PGCGROWTHCONDITIONS SRR4421264 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All strains were grown shaking at <Temp> 37 °C </Temp> in 200mL cultures of <Med> MOPS complete-glucose liquid media </Med> ( unless otherwise annotated ) in 1L flasks and cells were harvested at OD ( 420nm ) between 0.4 - 0.6 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE88725 Global analysis of translation termination in E. coli using release factor manipulations GSM2344785 WT_exp3_mRNA 28301469 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88725/GSE88725.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All strains were grown shaking at 37°C in 200mL cultures of MOPS complete-glucose liquid media (unless otherwise annotated) in 1L flasks and cells were harvested at OD(420nm) between 0.4 - 0.6 PGCGROWTHCONDITIONS SRR4421265SRR4421266 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All strains were grown shaking at <Temp> 37 °C </Temp> in 200mL cultures of <Med> MOPS complete-glucose liquid media </Med> ( unless otherwise annotated ) in 1L flasks and cells were harvested at OD ( 420nm ) between 0.4 - 0.6 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE88725 Global analysis of translation termination in E. coli using release factor manipulations GSM2344786 WT_exp4_mRNA 28301469 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88725/GSE88725.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All strains were grown shaking at 37°C in 200mL cultures of MOPS complete-glucose liquid media (unless otherwise annotated) in 1L flasks and cells were harvested at OD(420nm) between 0.4 - 0.6 PGCGROWTHCONDITIONS SRR4421267 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All strains were grown shaking at <Temp> 37 °C </Temp> in 200mL cultures of <Med> MOPS complete-glucose liquid media </Med> ( unless otherwise annotated ) in 1L flasks and cells were harvested at OD ( 420nm ) between 0.4 - 0.6 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE88725 Global analysis of translation termination in E. coli using release factor manipulations GSM2344787 ∆RF3_exp1_mRNA 28301469 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88725/GSE88725.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All strains were grown shaking at 37°C in 200mL cultures of MOPS complete-glucose liquid media (unless otherwise annotated) in 1L flasks and cells were harvested at OD(420nm) between 0.4 - 0.6 PGCGROWTHCONDITIONS SRR4421268 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All strains were grown shaking at <Temp> 37 °C </Temp> in 200mL cultures of <Med> MOPS complete-glucose liquid media </Med> ( unless otherwise annotated ) in 1L flasks and cells were harvested at OD ( 420nm ) between 0.4 - 0.6 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE88725 Global analysis of translation termination in E. coli using release factor manipulations GSM2344788 ∆RF3_exp3_mRNA 28301469 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88725/GSE88725.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All strains were grown shaking at 37°C in 200mL cultures of MOPS complete-glucose liquid media (unless otherwise annotated) in 1L flasks and cells were harvested at OD(420nm) between 0.4 - 0.6 PGCGROWTHCONDITIONS SRR4421269SRR4421270 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All strains were grown shaking at <Temp> 37 °C </Temp> in 200mL cultures of <Med> MOPS complete-glucose liquid media </Med> ( unless otherwise annotated ) in 1L flasks and cells were harvested at OD ( 420nm ) between 0.4 - 0.6 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE88725 Global analysis of translation termination in E. coli using release factor manipulations GSM2344789 RF2*_exp1_mRNA 28301469 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88725/GSE88725.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All strains were grown shaking at 37°C in 200mL cultures of MOPS complete-glucose liquid media (unless otherwise annotated) in 1L flasks and cells were harvested at OD(420nm) between 0.4 - 0.6 PGCGROWTHCONDITIONS SRR4421271 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All strains were grown shaking at <Temp> 37 °C </Temp> in 200mL cultures of <Med> MOPS complete-glucose liquid media </Med> ( unless otherwise annotated ) in 1L flasks and cells were harvested at OD ( 420nm ) between 0.4 - 0.6 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE88725 Global analysis of translation termination in E. coli using release factor manipulations GSM2344790 RF2*_exp3_mRNA 28301469 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88725/GSE88725.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All strains were grown shaking at 37°C in 200mL cultures of MOPS complete-glucose liquid media (unless otherwise annotated) in 1L flasks and cells were harvested at OD(420nm) between 0.4 - 0.6 PGCGROWTHCONDITIONS SRR4421272SRR4421273 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All strains were grown shaking at <Temp> 37 °C </Temp> in 200mL cultures of <Med> MOPS complete-glucose liquid media </Med> ( unless otherwise annotated ) in 1L flasks and cells were harvested at OD ( 420nm ) between 0.4 - 0.6 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE88725 Global analysis of translation termination in E. coli using release factor manipulations GSM2344791 RF2*∆RF3_exp2_repA_mRNA 28301469 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88725/GSE88725.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All strains were grown shaking at 37°C in 200mL cultures of MOPS complete-glucose liquid media (unless otherwise annotated) in 1L flasks and cells were harvested at OD(420nm) between 0.4 - 0.6 PGCGROWTHCONDITIONS SRR4421274 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All strains were grown shaking at <Temp> 37 °C </Temp> in 200mL cultures of <Med> MOPS complete-glucose liquid media </Med> ( unless otherwise annotated ) in 1L flasks and cells were harvested at OD ( 420nm ) between 0.4 - 0.6 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE88725 Global analysis of translation termination in E. coli using release factor manipulations GSM2344792 RF2*∆RF3_exp2_repB_mRNA 28301469 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88725/GSE88725.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All strains were grown shaking at 37°C in 200mL cultures of MOPS complete-glucose liquid media (unless otherwise annotated) in 1L flasks and cells were harvested at OD(420nm) between 0.4 - 0.6 PGCGROWTHCONDITIONS SRR4421275 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All strains were grown shaking at <Temp> 37 °C </Temp> in 200mL cultures of <Med> MOPS complete-glucose liquid media </Med> ( unless otherwise annotated ) in 1L flasks and cells were harvested at OD ( 420nm ) between 0.4 - 0.6 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE88725 Global analysis of translation termination in E. coli using release factor manipulations GSM2344793 RF2*∆RF3_exp3_mRNA 28301469 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88725/GSE88725.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All strains were grown shaking at 37°C in 200mL cultures of MOPS complete-glucose liquid media (unless otherwise annotated) in 1L flasks and cells were harvested at OD(420nm) between 0.4 - 0.6 PGCGROWTHCONDITIONS SRR4421276SRR4421277 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All strains were grown shaking at <Temp> 37 °C </Temp> in 200mL cultures of <Med> MOPS complete-glucose liquid media </Med> ( unless otherwise annotated ) in 1L flasks and cells were harvested at OD ( 420nm ) between 0.4 - 0.6 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE88725 Global analysis of translation termination in E. coli using release factor manipulations GSM2344794 RF2*∆RF3_exp4_repA_mRNA 28301469 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88725/GSE88725.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All strains were grown shaking at 37°C in 200mL cultures of MOPS complete-glucose liquid media (unless otherwise annotated) in 1L flasks and cells were harvested at OD(420nm) between 0.4 - 0.6 PGCGROWTHCONDITIONS SRR4421278 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All strains were grown shaking at <Temp> 37 °C </Temp> in 200mL cultures of <Med> MOPS complete-glucose liquid media </Med> ( unless otherwise annotated ) in 1L flasks and cells were harvested at OD ( 420nm ) between 0.4 - 0.6 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE88725 Global analysis of translation termination in E. coli using release factor manipulations GSM2344795 RF2*∆RF3_exp4_repB_mRNA 28301469 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88725/GSE88725.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All strains were grown shaking at 37°C in 200mL cultures of MOPS complete-glucose liquid media (unless otherwise annotated) in 1L flasks and cells were harvested at OD(420nm) between 0.4 - 0.6 PGCGROWTHCONDITIONS SRR4421279 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All strains were grown shaking at <Temp> 37 °C </Temp> in 200mL cultures of <Med> MOPS complete-glucose liquid media </Med> ( unless otherwise annotated ) in 1L flasks and cells were harvested at OD ( 420nm ) between 0.4 - 0.6 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE88725 Global analysis of translation termination in E. coli using release factor manipulations GSM2344809 WT_minimal_mRNA 28301469 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88725/GSE88725.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All strains were grown shaking at 37°C in 200mL cultures of MOPS complete-glucose liquid media (unless otherwise annotated) in 1L flasks and cells were harvested at OD(420nm) between 0.4 - 0.6 PGCGROWTHCONDITIONS SRR4421297 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C All strains were grown shaking at <Temp> 37 °C </Temp> in 200mL cultures of <Med> MOPS complete-glucose liquid media </Med> ( unless otherwise annotated ) in 1L flasks and cells were harvested at OD ( 420nm ) between 0.4 - 0.6 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE88725 Global analysis of translation termination in E. coli using release factor manipulations GSM2344810 ∆RF3_minimal_mRNA 28301469 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88725/GSE88725.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt All strains were grown shaking at 37°C in 200mL cultures of MOPS complete-glucose liquid media (unless otherwise annotated) in 1L flasks and cells were harvested at OD(420nm) between 0.4 - 0.6 PGCGROWTHCONDITIONS SRR4421298 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349915 Tube state 1 (IPTG-/aTc-/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427755 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349916 Tube state 2 (IPTG+/aTc-/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427756 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349917 Tube state 3 (IPTG-/aTc+/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427757 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349918 Tube state 4 (IPTG+/aTc+/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427758 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349919 Tube state 5 (IPTG-/aTc-/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427759 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349920 Tube state 6 (IPTG+/aTc-/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427760 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349921 Tube state 7 (IPTG-/aTc+/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427761 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349922 Tube state 8 (IPTG+/aTc+/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427762 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349923 Flask state 1 (IPTG-/aTc-/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427763 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349924 Flask state 2 (IPTG+/aTc-/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427764 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349925 Flask state 3 (IPTG-/aTc+/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427765 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349926 Flask state 4 (IPTG+/aTc+/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427766 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349927 Flask state 5 (IPTG-/aTc-/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427767 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349928 Flask state 6 (IPTG+/aTc-/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427768 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349929 Flask state 7 (IPTG-/aTc+/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427769 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349930 Flask state 8 (IPTG+/aTc+/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427770 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT and ΔompR were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with <Supp> 0.3 M of NaCl </Supp> at mid-log pahse for <Supp> 30 min </Supp> with agitation . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE88980 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GSM2356687 WT NaCl 1 28526842 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88980/GSE88980.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and ΔompR were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 0.3 M of NaCl at mid-log pahse for 30 min with agitation. PGCGROWTHCONDITIONS SRR4435464 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT and ΔompR were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with <Supp> 0.3 M of NaCl </Supp> at mid-log pahse for <Supp> 30 min </Supp> with agitation . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE88980 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GSM2356688 WT NaCl 2 28526842 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88980/GSE88980.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and ΔompR were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 0.3 M of NaCl at mid-log pahse for 30 min with agitation. PGCGROWTHCONDITIONS SRR4435465 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT and ΔompR were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with <Supp> 0.3 M of NaCl </Supp> at mid-log pahse for <Supp> 30 min </Supp> with agitation . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE88980 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GSM2356689 ΔompR NaCl 1 28526842 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88980/GSE88980.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and ΔompR were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 0.3 M of NaCl at mid-log pahse for 30 min with agitation. PGCGROWTHCONDITIONS SRR4435466 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K-12 MG1655 WT and ΔompR were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with <Supp> 0.3 M of NaCl </Supp> at mid-log pahse for <Supp> 30 min </Supp> with agitation . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE88980 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GSM2356690 ΔompR NaCl 2 28526842 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88980/GSE88980.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and ΔompR were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 0.3 M of NaCl at mid-log pahse for 30 min with agitation. PGCGROWTHCONDITIONS SRR4435467 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411669 chemostat STR-PFR culture STR 5min, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071589 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411670 chemostat STR-PFR culture STR 10min, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071590 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411671 chemostat STR-PFR culture STR 25min, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071591 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411672 chemostat STR-PFR culture STR 45min, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071592 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411673 chemostat STR-PFR culture STR 75min, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071593 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411674 chemostat STR-PFR culture STR 120min, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071594 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411675 chemostat STR-PFR culture STR 210min, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071595 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411676 chemostat STR-PFR culture STR 330min, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071596 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411677 chemostat STR-PFR culture STR 25h, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071597 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411678 chemostat STR-PFR culture STR 26h, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071598 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411679 chemostat STR-PFR culture STR 28h, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071599 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411680 chemostat STR culture STR reference 1, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071600 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411681 chemostat STR culture STR reference 2, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071601 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411682 chemostat STR culture STR reference 3, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071602 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411683 chemostat STR-PFR culture PFR P5 5min, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071603 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411684 chemostat STR-PFR culture PFR P5 10min, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071604 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411685 chemostat STR-PFR culture PFR P5 25min, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071605 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411686 chemostat STR-PFR culture PFR P3 25min, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071606 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411687 chemostat STR-PFR culture PFR P1 25min, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071607 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411688 chemostat STR-PFR culture PFR P5 45min, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071608 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411689 chemostat STR-PFR culture PFR P5 120min, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071609 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411690 chemostat STR-PFR culture PFR P3 120min, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071610 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411691 chemostat STR-PFR culture PFR P1 120min, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071611 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411692 chemostat STR-PFR culture PFR P5 210min, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071612 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411693 chemostat STR-PFR culture PFR P5 25h, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071613 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411694 chemostat STR-PFR culture PFR P3 28h, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071614 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411695 chemostat STR-PFR culture PFR P5 28h, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071615 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411696 chemostat STR-PFR culture PFR P1 28h, rep1 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071616 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411697 chemostat STR-PFR culture STR 5min, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071617 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411698 chemostat STR-PFR culture STR 10min, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071618 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411699 chemostat STR-PFR culture STR 25min, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071619 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411700 chemostat STR-PFR culture STR 45min, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071620 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411701 chemostat STR-PFR culture STR 75min, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071621 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411702 chemostat STR-PFR culture STR 120min, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071622 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411703 chemostat STR-PFR culture STR 210min, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071623 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411704 chemostat STR-PFR culture STR 330min, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071624 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411705 chemostat STR-PFR culture STR 25h, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071625 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411706 chemostat STR-PFR culture STR 26h, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071626 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411707 chemostat STR-PFR culture STR 28h, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071627 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411708 chemostat STR culture STR reference 1 , rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071628 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411709 chemostat STR culture STR reference 2 , rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071629 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411710 chemostat STR culture STR reference 3 , rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071630 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411711 chemostat STR-PFR culture PFR P5 5min, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071631 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411712 chemostat STR-PFR culture PFR P5 10min, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071632 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411713 chemostat STR-PFR culture PFR P5 25min, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071633 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411714 chemostat STR-PFR culture PFR P3 25min, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071634 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411715 chemostat STR-PFR culture PFR P1 25min, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071635 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411716 chemostat STR-PFR culture PFR P5 45min, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071636 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411717 chemostat STR-PFR culture PFR P5 75min, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071637 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411718 chemostat STR-PFR culture PFR P5 120min, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071638 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411719 chemostat STR-PFR culture PFR P3 120min, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071639 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411720 chemostat STR-PFR culture PFR P1 120min, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071640 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411721 chemostat STR-PFR culture PFR P5 210min, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071641 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411722 chemostat STR-PFR culture PFR P5 330min, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071642 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411723 chemostat STR-PFR culture PFR P5 25h, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071643 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411724 chemostat STR-PFR culture PFR P5 26h, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071644 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411725 chemostat STR-PFR culture PFR P5 28h, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071645 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411726 chemostat STR-PFR culture PFR P3 28h, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071646 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli K12 substrain W3110 was used in this study . Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR ( 3L ) with a recycle loop ( PFR ) in defined medium at pH7 and <Temp> 37 °C </Temp> . Bioreactor cultivations were carried out with a minimal medium containing ( per liter ) 19.0 g glucose , 1.0 g NaH2PO4 . · 2 H2O , 2.6 g K2HPO4 , 3.8 g ( NH4 ) 2SO4 , and a trace element solution ( 0.11 g Na3C6H5O7 , 0.00835 g FeCl3 · 6 H2O , 0.00009 g ZnSO4 · 7 H2O , 0.00005 g MnSO4 · H2O , 0.0008 g CuSO4 · 5 H2O , 0.00009 g CoCl2 · 6 H2O , 0.0044 g CaCl2 · 2 H2O , 0.1 g MgSO4 · 7 H2O ) . The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate . First , a reference steady state ( µ = 0.2 h − 1 ) was established in the STR and sampled three times during a 16 h period following establishment of the steady state . Then , the PFR was added and samples for RNA-sequencing were aquired . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE90743 RNA-seq of Escherichia coli under fluctuating ammonia availability (limitation-depletion) GSM2411727 chemostat STR-PFR culture PFR P1 28h, rep2 2844739128702020 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90743/GSE90743.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 substrain W3110 was used in this study. Bioreactor cultivations were performed using a two-compartment bioreactor system consisting of an STR (3L) with a recycle loop (PFR) in defined medium at pH7 and 37°C. Bioreactor cultivations were carried out with a minimal medium containing (per liter) 19.0 g glucose, 1.0 g NaH2PO4.· 2 H2O, 2.6 g K2HPO4, 3.8 g (NH4)2SO4, and a trace element solution (0.11 g Na3C6H5O7, 0.00835 g FeCl3· 6 H2O, 0.00009 g ZnSO4· 7 H2O, 0.00005 g MnSO4· H2O, 0.0008 g CuSO4· 5 H2O, 0.00009 g CoCl2· 6 H2O, 0.0044 g CaCl2· 2 H2O, 0.1 g MgSO4· 7 H2O). The STR-PFR was operated as a chemostat with ammonia as the growth-limiting substrate. First, a reference steady state (µ=0.2 h−1) was established in the STR and sampled three times during a 16 h period following establishment of the steady state. Then, the PFR was added and samples for RNA-sequencing were aquired. PGCGROWTHCONDITIONS SRR5071647 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli TolC cells were grown o/n in LB medium at <Temp> 37 °C </Temp> and 200 rpm . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE93125 Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton GSM2445135 Eco_TolC_0min_A 28959742 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE93125/GSE93125.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli TolC cells were grown o/n in LB medium at 37°C and 200 rpm. PGCGROWTHCONDITIONS SRR5143857 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli TolC cells were grown o/n in LB medium at <Temp> 37 °C </Temp> and 200 rpm . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE93125 Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton GSM2445138 Eco_TolC_0min_B 28959742 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE93125/GSE93125.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli TolC cells were grown o/n in LB medium at 37°C and 200 rpm. PGCGROWTHCONDITIONS SRR5143858 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli TolC cells were grown o/n in LB medium at <Temp> 37 °C </Temp> and 200 rpm . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE93125 Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton GSM2445140 Eco_TolC_5min_control_A 28959742 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE93125/GSE93125.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli TolC cells were grown o/n in LB medium at 37°C and 200 rpm. PGCGROWTHCONDITIONS SRR5143859 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli TolC cells were grown o/n in LB medium at <Temp> 37 °C </Temp> and 200 rpm . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE93125 Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton GSM2445142 Eco_TolC_5min_control_B 28959742 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE93125/GSE93125.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli TolC cells were grown o/n in LB medium at 37°C and 200 rpm. PGCGROWTHCONDITIONS SRR5143860 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli TolC cells were grown o/n in LB medium at <Temp> 37 °C </Temp> and 200 rpm . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE93125 Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton GSM2445144 Eco_TolC_5min_Carolacton_A 28959742 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE93125/GSE93125.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli TolC cells were grown o/n in LB medium at 37°C and 200 rpm. PGCGROWTHCONDITIONS SRR5143861 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli TolC cells were grown o/n in LB medium at <Temp> 37 °C </Temp> and 200 rpm . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE93125 Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton GSM2445145 Eco_TolC_5min_Carolacton_B 28959742 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE93125/GSE93125.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli TolC cells were grown o/n in LB medium at 37°C and 200 rpm. PGCGROWTHCONDITIONS SRR5143862 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli TolC cells were grown o/n in LB medium at <Temp> 37 °C </Temp> and 200 rpm . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE93125 Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton GSM2445147 Eco_TolC_15min_control_A 28959742 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE93125/GSE93125.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli TolC cells were grown o/n in LB medium at 37°C and 200 rpm. PGCGROWTHCONDITIONS SRR5143863 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli TolC cells were grown o/n in LB medium at <Temp> 37 °C </Temp> and 200 rpm . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE93125 Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton GSM2445149 Eco_TolC_15min_control_B 28959742 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE93125/GSE93125.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli TolC cells were grown o/n in LB medium at 37°C and 200 rpm. PGCGROWTHCONDITIONS SRR5143864 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli TolC cells were grown o/n in LB medium at <Temp> 37 °C </Temp> and 200 rpm . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE93125 Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton GSM2445151 Eco_TolC_15min_Carolacton_A 28959742 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE93125/GSE93125.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli TolC cells were grown o/n in LB medium at 37°C and 200 rpm. PGCGROWTHCONDITIONS SRR5143865 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli TolC cells were grown o/n in LB medium at <Temp> 37 °C </Temp> and 200 rpm . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE93125 Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton GSM2445153 Eco_TolC_15min_Carolacton_B 28959742 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE93125/GSE93125.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli TolC cells were grown o/n in LB medium at 37°C and 200 rpm. PGCGROWTHCONDITIONS SRR5143866 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli TolC cells were grown o/n in LB medium at <Temp> 37 °C </Temp> and 200 rpm . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE93125 Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton GSM2445155 Eco_TolC_30min_control_A 28959742 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE93125/GSE93125.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli TolC cells were grown o/n in LB medium at 37°C and 200 rpm. PGCGROWTHCONDITIONS SRR5143868 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli TolC cells were grown o/n in LB medium at <Temp> 37 °C </Temp> and 200 rpm . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE93125 Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton GSM2445157 Eco_TolC_30min_control_B 28959742 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE93125/GSE93125.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli TolC cells were grown o/n in LB medium at 37°C and 200 rpm. PGCGROWTHCONDITIONS SRR5143869 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli TolC cells were grown o/n in LB medium at <Temp> 37 °C </Temp> and 200 rpm . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE93125 Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton GSM2445159 Eco_TolC_30min_Carolacton_A 28959742 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE93125/GSE93125.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli TolC cells were grown o/n in LB medium at 37°C and 200 rpm. PGCGROWTHCONDITIONS SRR5143870 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C E. coli TolC cells were grown o/n in LB medium at <Temp> 37 °C </Temp> and 200 rpm . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE93125 Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton GSM2445161 Eco_TolC_30min_Carolacton_B 28959742 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE93125/GSE93125.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli TolC cells were grown o/n in LB medium at 37°C and 200 rpm. PGCGROWTHCONDITIONS SRR5143871 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C LB medium , 180 rpm shaking , at <Temp> 37 °C </Temp> , between exponential and <Phase> stationary phase </Phase> GPL23073 GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933) GSE94984 Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP GSM2493797 EHEC in LB Experiment 2 [RNA-Seq] 28245801 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE94984/GSE94984.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt LB medium, 180 rpm shaking, at 37°C, between exponential and stationary phase PGCGROWTHCONDITIONS SRR5266619 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516609 1A_MG_t0 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304286 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516610 2A_MG_t10 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304287 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516611 3A_MG_t30 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304288 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516612 4A_MG_t60 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304289 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516613 5A_MG+Hg_t10 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304290 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516614 6A_MG+Hg_t30 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304291 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516615 7A_MG+Hg_t60 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304292 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516616 8A_MG+PMA_t10 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304293 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516617 9A_MG+PMA_t30 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304294 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516618 10A_MG+PMA_t60 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304295 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516619 1B_MG_t0 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304296 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516620 2B_MG_t10 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304297 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516621 3B_MG_t30 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304298 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516622 4B_MG_t60 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304299 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516623 5B_MG+Hg_t10 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304300 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516624 6B_MG+Hg_t30 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304301 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516625 7B_MG+Hg_t60 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304302 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516626 8B_MG+PMA_t10 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304303 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516627 9B_MG+PMA_t30 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304304 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516628 10B_MG+PMA_t60 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304305 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516629 1C_MG_t0 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304306 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516630 2C_MG_t10 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304307 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516631 3C_MG_t30 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304308 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516632 4C_MG_t60 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304309 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516633 5C_MG+Hg_t10 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304310 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516634 6C_MG+Hg_t30 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304311 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516635 7C_MG+Hg_t60 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304312 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516636 8C_MG+PMA_t10 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304313 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516637 9C_MG+PMA_t30 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304314 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516638 10C_MG+PMA_t60 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304315 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757255 0x58 replicate 2 state 1 (IPTG-/aTc-/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985582 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757256 0x58 replicate 2 state 2 (IPTG+/aTc-/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985583 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757257 0x58 replicate 2 state 3 (IPTG-/aTc+/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985584 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757258 0x58 replicate 2 state 4 (IPTG+/aTc+/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985585 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757259 0x58 replicate 2 state 5 (IPTG-/aTc-/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985586 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757260 0x58 replicate 2 state 6 (IPTG+/aTc-/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985587 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757261 0x58 replicate 2 state 7 (IPTG-/aTc+/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985588 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757262 0x58 replicate 2 state 8 (IPTG+/aTc+/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985589 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757263 0x58 replicate 3 state 1 (IPTG-/aTc-/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985590 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757264 0x58 replicate 3 state 2 (IPTG+/aTc-/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985591 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757265 0x58 replicate 3 state 3 (IPTG-/aTc+/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985592 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757266 0x58 replicate 3 state 4 (IPTG+/aTc+/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985593 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757267 0x58 replicate 3 state 5 (IPTG-/aTc-/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985594 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757268 0x58 replicate 3 state 6 (IPTG+/aTc-/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985595 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757269 0x58 replicate 3 state 7 (IPTG-/aTc+/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985596 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757270 0x58 replicate 3 state 8 (IPTG+/aTc+/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985597 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757272 Control pAN1201 replicate 1 (IPTG-/aTc-/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985599 MCO000003199 37 °C Temperature True
growth_protocol 35.7 C Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757273 Control pAN1201 replicate 2 (IPTG-/aTc-/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985600 MCO000003199 37 °C Temperature True
treatment_protocol iptg Starting from single colonies , the following cultures were set up in triplicate for overnight incubation : GJ13507 , GJ13531 , and GJ13533 in glucose-minimal A ; and GJ13519 also in 0.2 % glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG , with the exception of the cultures of GJ13507 whose supplementation with <Supp> IPTG was </Supp> at 3 μM . The overnight-grown cultures were each subcultured into 20 ml <Med> of fresh medium </Med> of the same composition , with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder , and grown to an A600 of 0.4 to 0.45 , before the cells were harvested for making the RNA preparations GPL24020 GPL24020: Illumina NextSeq 500 (Escherichia coli K-12) GSE103937 Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli GSM2786596 WT glucose_replicate1 29474582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103937/GSE103937.soft.gz 100 67 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Starting from single colonies, the following cultures were set up in triplicate for overnight incubation: GJ13507, GJ13531, and GJ13533 in glucose-minimal A; and GJ13519 also in 0.2% glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG, with the exception of the cultures of GJ13507 whose supplementation with IPTG was at 3 μM. The overnight-grown cultures were each subcultured into 20 ml of fresh medium of the same composition, with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder, and grown to an A600 of 0.4 to 0.45, before the cells were harvested for making the RNA preparations PGCGROWTHCONDITIONS SRR6048166 MCO000002105 IPTG was Medium supplement True
treatment_protocol iptg Starting from single colonies , the following cultures were set up in triplicate for overnight incubation : GJ13507 , GJ13531 , and GJ13533 in glucose-minimal A ; and GJ13519 also in 0.2 % glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG , with the exception of the cultures of GJ13507 whose supplementation with <Supp> IPTG was </Supp> at 3 μM . The overnight-grown cultures were each subcultured into 20 ml <Med> of fresh medium </Med> of the same composition , with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder , and grown to an A600 of 0.4 to 0.45 , before the cells were harvested for making the RNA preparations GPL24020 GPL24020: Illumina NextSeq 500 (Escherichia coli K-12) GSE103937 Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli GSM2786597 WT glucose_replicate2 29474582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103937/GSE103937.soft.gz 100 67 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Starting from single colonies, the following cultures were set up in triplicate for overnight incubation: GJ13507, GJ13531, and GJ13533 in glucose-minimal A; and GJ13519 also in 0.2% glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG, with the exception of the cultures of GJ13507 whose supplementation with IPTG was at 3 μM. The overnight-grown cultures were each subcultured into 20 ml of fresh medium of the same composition, with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder, and grown to an A600 of 0.4 to 0.45, before the cells were harvested for making the RNA preparations PGCGROWTHCONDITIONS SRR6048167 MCO000002105 IPTG was Medium supplement True
treatment_protocol iptg Starting from single colonies , the following cultures were set up in triplicate for overnight incubation : GJ13507 , GJ13531 , and GJ13533 in glucose-minimal A ; and GJ13519 also in 0.2 % glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG , with the exception of the cultures of GJ13507 whose supplementation with <Supp> IPTG was </Supp> at 3 μM . The overnight-grown cultures were each subcultured into 20 ml <Med> of fresh medium </Med> of the same composition , with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder , and grown to an A600 of 0.4 to 0.45 , before the cells were harvested for making the RNA preparations GPL24020 GPL24020: Illumina NextSeq 500 (Escherichia coli K-12) GSE103937 Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli GSM2786598 WT glucose_replicate3 29474582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103937/GSE103937.soft.gz 100 67 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Starting from single colonies, the following cultures were set up in triplicate for overnight incubation: GJ13507, GJ13531, and GJ13533 in glucose-minimal A; and GJ13519 also in 0.2% glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG, with the exception of the cultures of GJ13507 whose supplementation with IPTG was at 3 μM. The overnight-grown cultures were each subcultured into 20 ml of fresh medium of the same composition, with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder, and grown to an A600 of 0.4 to 0.45, before the cells were harvested for making the RNA preparations PGCGROWTHCONDITIONS SRR6048168 MCO000002105 IPTG was Medium supplement True
treatment_protocol iptg Starting from single colonies , the following cultures were set up in triplicate for overnight incubation : GJ13507 , GJ13531 , and GJ13533 in glucose-minimal A ; and GJ13519 also in 0.2 % glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG , with the exception of the cultures of GJ13507 whose supplementation with <Supp> IPTG was </Supp> at 3 μM . The overnight-grown cultures were each subcultured into 20 ml <Med> of fresh medium </Med> of the same composition , with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder , and grown to an A600 of 0.4 to 0.45 , before the cells were harvested for making the RNA preparations GPL24020 GPL24020: Illumina NextSeq 500 (Escherichia coli K-12) GSE103937 Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli GSM2786599 WT_glycerol_replicate1 29474582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103937/GSE103937.soft.gz 100 67 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Starting from single colonies, the following cultures were set up in triplicate for overnight incubation: GJ13507, GJ13531, and GJ13533 in glucose-minimal A; and GJ13519 also in 0.2% glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG, with the exception of the cultures of GJ13507 whose supplementation with IPTG was at 3 μM. The overnight-grown cultures were each subcultured into 20 ml of fresh medium of the same composition, with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder, and grown to an A600 of 0.4 to 0.45, before the cells were harvested for making the RNA preparations PGCGROWTHCONDITIONS SRR6048169 MCO000002105 IPTG was Medium supplement True
treatment_protocol iptg Starting from single colonies , the following cultures were set up in triplicate for overnight incubation : GJ13507 , GJ13531 , and GJ13533 in glucose-minimal A ; and GJ13519 also in 0.2 % glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG , with the exception of the cultures of GJ13507 whose supplementation with <Supp> IPTG was </Supp> at 3 μM . The overnight-grown cultures were each subcultured into 20 ml <Med> of fresh medium </Med> of the same composition , with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder , and grown to an A600 of 0.4 to 0.45 , before the cells were harvested for making the RNA preparations GPL24020 GPL24020: Illumina NextSeq 500 (Escherichia coli K-12) GSE103937 Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli GSM2786600 WT_glycerol_replicate2 29474582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103937/GSE103937.soft.gz 100 67 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Starting from single colonies, the following cultures were set up in triplicate for overnight incubation: GJ13507, GJ13531, and GJ13533 in glucose-minimal A; and GJ13519 also in 0.2% glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG, with the exception of the cultures of GJ13507 whose supplementation with IPTG was at 3 μM. The overnight-grown cultures were each subcultured into 20 ml of fresh medium of the same composition, with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder, and grown to an A600 of 0.4 to 0.45, before the cells were harvested for making the RNA preparations PGCGROWTHCONDITIONS SRR6048170 MCO000002105 IPTG was Medium supplement True
treatment_protocol iptg Starting from single colonies , the following cultures were set up in triplicate for overnight incubation : GJ13507 , GJ13531 , and GJ13533 in glucose-minimal A ; and GJ13519 also in 0.2 % glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG , with the exception of the cultures of GJ13507 whose supplementation with <Supp> IPTG was </Supp> at 3 μM . The overnight-grown cultures were each subcultured into 20 ml <Med> of fresh medium </Med> of the same composition , with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder , and grown to an A600 of 0.4 to 0.45 , before the cells were harvested for making the RNA preparations GPL24020 GPL24020: Illumina NextSeq 500 (Escherichia coli K-12) GSE103937 Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli GSM2786601 WT_glycerol_replicate3 29474582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103937/GSE103937.soft.gz 100 67 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Starting from single colonies, the following cultures were set up in triplicate for overnight incubation: GJ13507, GJ13531, and GJ13533 in glucose-minimal A; and GJ13519 also in 0.2% glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG, with the exception of the cultures of GJ13507 whose supplementation with IPTG was at 3 μM. The overnight-grown cultures were each subcultured into 20 ml of fresh medium of the same composition, with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder, and grown to an A600 of 0.4 to 0.45, before the cells were harvested for making the RNA preparations PGCGROWTHCONDITIONS SRR6048171 MCO000002105 IPTG was Medium supplement True
treatment_protocol iptg Starting from single colonies , the following cultures were set up in triplicate for overnight incubation : GJ13507 , GJ13531 , and GJ13533 in glucose-minimal A ; and GJ13519 also in 0.2 % glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG , with the exception of the cultures of GJ13507 whose supplementation with <Supp> IPTG was </Supp> at 3 μM . The overnight-grown cultures were each subcultured into 20 ml <Med> of fresh medium </Med> of the same composition , with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder , and grown to an A600 of 0.4 to 0.45 , before the cells were harvested for making the RNA preparations GPL24020 GPL24020: Illumina NextSeq 500 (Escherichia coli K-12) GSE103937 Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli GSM2786602 WT UvsW_replicate1 29474582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103937/GSE103937.soft.gz 100 67 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Starting from single colonies, the following cultures were set up in triplicate for overnight incubation: GJ13507, GJ13531, and GJ13533 in glucose-minimal A; and GJ13519 also in 0.2% glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG, with the exception of the cultures of GJ13507 whose supplementation with IPTG was at 3 μM. The overnight-grown cultures were each subcultured into 20 ml of fresh medium of the same composition, with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder, and grown to an A600 of 0.4 to 0.45, before the cells were harvested for making the RNA preparations PGCGROWTHCONDITIONS SRR6048172 MCO000002105 IPTG was Medium supplement True
treatment_protocol iptg Starting from single colonies , the following cultures were set up in triplicate for overnight incubation : GJ13507 , GJ13531 , and GJ13533 in glucose-minimal A ; and GJ13519 also in 0.2 % glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG , with the exception of the cultures of GJ13507 whose supplementation with <Supp> IPTG was </Supp> at 3 μM . The overnight-grown cultures were each subcultured into 20 ml <Med> of fresh medium </Med> of the same composition , with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder , and grown to an A600 of 0.4 to 0.45 , before the cells were harvested for making the RNA preparations GPL24020 GPL24020: Illumina NextSeq 500 (Escherichia coli K-12) GSE103937 Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli GSM2786603 WT UvsW_replicate2 29474582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103937/GSE103937.soft.gz 100 67 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Starting from single colonies, the following cultures were set up in triplicate for overnight incubation: GJ13507, GJ13531, and GJ13533 in glucose-minimal A; and GJ13519 also in 0.2% glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG, with the exception of the cultures of GJ13507 whose supplementation with IPTG was at 3 μM. The overnight-grown cultures were each subcultured into 20 ml of fresh medium of the same composition, with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder, and grown to an A600 of 0.4 to 0.45, before the cells were harvested for making the RNA preparations PGCGROWTHCONDITIONS SRR6048173 MCO000002105 IPTG was Medium supplement True
treatment_protocol iptg Starting from single colonies , the following cultures were set up in triplicate for overnight incubation : GJ13507 , GJ13531 , and GJ13533 in glucose-minimal A ; and GJ13519 also in 0.2 % glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG , with the exception of the cultures of GJ13507 whose supplementation with <Supp> IPTG was </Supp> at 3 μM . The overnight-grown cultures were each subcultured into 20 ml <Med> of fresh medium </Med> of the same composition , with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder , and grown to an A600 of 0.4 to 0.45 , before the cells were harvested for making the RNA preparations GPL24020 GPL24020: Illumina NextSeq 500 (Escherichia coli K-12) GSE103937 Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli GSM2786604 WT UvsW_replicate3 29474582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103937/GSE103937.soft.gz 100 67 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Starting from single colonies, the following cultures were set up in triplicate for overnight incubation: GJ13507, GJ13531, and GJ13533 in glucose-minimal A; and GJ13519 also in 0.2% glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG, with the exception of the cultures of GJ13507 whose supplementation with IPTG was at 3 μM. The overnight-grown cultures were each subcultured into 20 ml of fresh medium of the same composition, with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder, and grown to an A600 of 0.4 to 0.45, before the cells were harvested for making the RNA preparations PGCGROWTHCONDITIONS SRR6048174 MCO000002105 IPTG was Medium supplement True
treatment_protocol iptg Starting from single colonies , the following cultures were set up in triplicate for overnight incubation : GJ13507 , GJ13531 , and GJ13533 in glucose-minimal A ; and GJ13519 also in 0.2 % glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG , with the exception of the cultures of GJ13507 whose supplementation with <Supp> IPTG was </Supp> at 3 μM . The overnight-grown cultures were each subcultured into 20 ml <Med> of fresh medium </Med> of the same composition , with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder , and grown to an A600 of 0.4 to 0.45 , before the cells were harvested for making the RNA preparations GPL24020 GPL24020: Illumina NextSeq 500 (Escherichia coli K-12) GSE103937 Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli GSM2786605 ∆rho UvsW_replicate1 29474582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103937/GSE103937.soft.gz 100 67 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Starting from single colonies, the following cultures were set up in triplicate for overnight incubation: GJ13507, GJ13531, and GJ13533 in glucose-minimal A; and GJ13519 also in 0.2% glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG, with the exception of the cultures of GJ13507 whose supplementation with IPTG was at 3 μM. The overnight-grown cultures were each subcultured into 20 ml of fresh medium of the same composition, with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder, and grown to an A600 of 0.4 to 0.45, before the cells were harvested for making the RNA preparations PGCGROWTHCONDITIONS SRR6048175 MCO000002105 IPTG was Medium supplement True
treatment_protocol iptg Starting from single colonies , the following cultures were set up in triplicate for overnight incubation : GJ13507 , GJ13531 , and GJ13533 in glucose-minimal A ; and GJ13519 also in 0.2 % glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG , with the exception of the cultures of GJ13507 whose supplementation with <Supp> IPTG was </Supp> at 3 μM . The overnight-grown cultures were each subcultured into 20 ml <Med> of fresh medium </Med> of the same composition , with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder , and grown to an A600 of 0.4 to 0.45 , before the cells were harvested for making the RNA preparations GPL24020 GPL24020: Illumina NextSeq 500 (Escherichia coli K-12) GSE103937 Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli GSM2786606 ∆rho UvsW_replicate2 29474582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103937/GSE103937.soft.gz 100 67 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Starting from single colonies, the following cultures were set up in triplicate for overnight incubation: GJ13507, GJ13531, and GJ13533 in glucose-minimal A; and GJ13519 also in 0.2% glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG, with the exception of the cultures of GJ13507 whose supplementation with IPTG was at 3 μM. The overnight-grown cultures were each subcultured into 20 ml of fresh medium of the same composition, with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder, and grown to an A600 of 0.4 to 0.45, before the cells were harvested for making the RNA preparations PGCGROWTHCONDITIONS SRR6048176 MCO000002105 IPTG was Medium supplement True
treatment_protocol iptg Starting from single colonies , the following cultures were set up in triplicate for overnight incubation : GJ13507 , GJ13531 , and GJ13533 in glucose-minimal A ; and GJ13519 also in 0.2 % glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG , with the exception of the cultures of GJ13507 whose supplementation with <Supp> IPTG was </Supp> at 3 μM . The overnight-grown cultures were each subcultured into 20 ml <Med> of fresh medium </Med> of the same composition , with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder , and grown to an A600 of 0.4 to 0.45 , before the cells were harvested for making the RNA preparations GPL24020 GPL24020: Illumina NextSeq 500 (Escherichia coli K-12) GSE103937 Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli GSM2786607 ∆rho UvsW_replicate3 29474582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103937/GSE103937.soft.gz 100 67 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Starting from single colonies, the following cultures were set up in triplicate for overnight incubation: GJ13507, GJ13531, and GJ13533 in glucose-minimal A; and GJ13519 also in 0.2% glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG, with the exception of the cultures of GJ13507 whose supplementation with IPTG was at 3 μM. The overnight-grown cultures were each subcultured into 20 ml of fresh medium of the same composition, with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder, and grown to an A600 of 0.4 to 0.45, before the cells were harvested for making the RNA preparations PGCGROWTHCONDITIONS SRR6048177 MCO000002105 IPTG was Medium supplement True
treatment_protocol iptg Starting from single colonies , the following cultures were set up in triplicate for overnight incubation : GJ13507 , GJ13531 , and GJ13533 in glucose-minimal A ; and GJ13519 also in 0.2 % glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG , with the exception of the cultures of GJ13507 whose supplementation with <Supp> IPTG was </Supp> at 3 μM . The overnight-grown cultures were each subcultured into 20 ml <Med> of fresh medium </Med> of the same composition , with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder , and grown to an A600 of 0.4 to 0.45 , before the cells were harvested for making the RNA preparations GPL24020 GPL24020: Illumina NextSeq 500 (Escherichia coli K-12) GSE103937 Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli GSM2786608 ∆nusG UvsW_replicate1 29474582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103937/GSE103937.soft.gz 100 67 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Starting from single colonies, the following cultures were set up in triplicate for overnight incubation: GJ13507, GJ13531, and GJ13533 in glucose-minimal A; and GJ13519 also in 0.2% glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG, with the exception of the cultures of GJ13507 whose supplementation with IPTG was at 3 μM. The overnight-grown cultures were each subcultured into 20 ml of fresh medium of the same composition, with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder, and grown to an A600 of 0.4 to 0.45, before the cells were harvested for making the RNA preparations PGCGROWTHCONDITIONS SRR6048178 MCO000002105 IPTG was Medium supplement True
treatment_protocol iptg Starting from single colonies , the following cultures were set up in triplicate for overnight incubation : GJ13507 , GJ13531 , and GJ13533 in glucose-minimal A ; and GJ13519 also in 0.2 % glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG , with the exception of the cultures of GJ13507 whose supplementation with <Supp> IPTG was </Supp> at 3 μM . The overnight-grown cultures were each subcultured into 20 ml <Med> of fresh medium </Med> of the same composition , with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder , and grown to an A600 of 0.4 to 0.45 , before the cells were harvested for making the RNA preparations GPL24020 GPL24020: Illumina NextSeq 500 (Escherichia coli K-12) GSE103937 Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli GSM2786609 ∆nusG UvsW_replicate2 29474582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103937/GSE103937.soft.gz 100 67 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Starting from single colonies, the following cultures were set up in triplicate for overnight incubation: GJ13507, GJ13531, and GJ13533 in glucose-minimal A; and GJ13519 also in 0.2% glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG, with the exception of the cultures of GJ13507 whose supplementation with IPTG was at 3 μM. The overnight-grown cultures were each subcultured into 20 ml of fresh medium of the same composition, with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder, and grown to an A600 of 0.4 to 0.45, before the cells were harvested for making the RNA preparations PGCGROWTHCONDITIONS SRR6048179 MCO000002105 IPTG was Medium supplement True
treatment_protocol iptg Starting from single colonies , the following cultures were set up in triplicate for overnight incubation : GJ13507 , GJ13531 , and GJ13533 in glucose-minimal A ; and GJ13519 also in 0.2 % glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG , with the exception of the cultures of GJ13507 whose supplementation with <Supp> IPTG was </Supp> at 3 μM . The overnight-grown cultures were each subcultured into 20 ml <Med> of fresh medium </Med> of the same composition , with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder , and grown to an A600 of 0.4 to 0.45 , before the cells were harvested for making the RNA preparations GPL24020 GPL24020: Illumina NextSeq 500 (Escherichia coli K-12) GSE103937 Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli GSM2786610 ∆nusG UvsW_replicate3 29474582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103937/GSE103937.soft.gz 100 67 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Starting from single colonies, the following cultures were set up in triplicate for overnight incubation: GJ13507, GJ13531, and GJ13533 in glucose-minimal A; and GJ13519 also in 0.2% glycerol-minimal A. All the cultures were supplemented with 200 μM IPTG, with the exception of the cultures of GJ13507 whose supplementation with IPTG was at 3 μM. The overnight-grown cultures were each subcultured into 20 ml of fresh medium of the same composition, with an inoculum of 1:50 for GJ13507 and GJ13531 and of 1:100 for the remainder, and grown to an A600 of 0.4 to 0.45, before the cells were harvested for making the RNA preparations PGCGROWTHCONDITIONS SRR6048180 MCO000002105 IPTG was Medium supplement True
characteristics exponential phase growth phase : <Anti> Mid </Anti> <Phase> exponential </Phase> GPL21222-GPL21726 GPL21222: Illumina NextSeq 500 (Escherichia coli). GPL21726: Ion Torrent Proton (Escherichia coli) GSE104504 Crosstalk between global regulators CRP and FIS in E. coli. GSM2802560 fis.rep1.me 29205228 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE104504/GSE104504.soft.gz 100 79 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: Mid exponential PGCGROWTHCONDITIONS SRR6125551 MCO000002864 exponential Growth phase True
characteristics exponential phase growth phase : <Anti> Mid </Anti> <Phase> exponential </Phase> GPL21222-GPL21726 GPL21222: Illumina NextSeq 500 (Escherichia coli). GPL21726: Ion Torrent Proton (Escherichia coli) GSE104504 Crosstalk between global regulators CRP and FIS in E. coli. GSM2802561 fis.rep2.me 29205228 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE104504/GSE104504.soft.gz 100 79 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: Mid exponential PGCGROWTHCONDITIONS SRR6125552 MCO000002864 exponential Growth phase True
characteristics exponential phase growth phase : <Anti> Mid </Anti> <Phase> exponential </Phase> GPL21222-GPL21726 GPL21222: Illumina NextSeq 500 (Escherichia coli). GPL21726: Ion Torrent Proton (Escherichia coli) GSE104504 Crosstalk between global regulators CRP and FIS in E. coli. GSM2802565 cya.rep2.me 29205228 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE104504/GSE104504.soft.gz 100 79 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: Mid exponential PGCGROWTHCONDITIONS SRR6125556 MCO000002864 exponential Growth phase True
characteristics exponential phase growth phase : <Anti> Mid </Anti> <Phase> exponential </Phase> GPL21222-GPL21726 GPL21222: Illumina NextSeq 500 (Escherichia coli). GPL21726: Ion Torrent Proton (Escherichia coli) GSE104504 Crosstalk between global regulators CRP and FIS in E. coli. GSM2802566 wt.rep1.me 29205228 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE104504/GSE104504.soft.gz 100 79 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: Mid exponential PGCGROWTHCONDITIONS SRR6125557 MCO000002864 exponential Growth phase True
characteristics exponential phase growth phase : <Anti> Mid </Anti> <Phase> exponential </Phase> GPL21222-GPL21726 GPL21222: Illumina NextSeq 500 (Escherichia coli). GPL21726: Ion Torrent Proton (Escherichia coli) GSE104504 Crosstalk between global regulators CRP and FIS in E. coli. GSM2802567 wt.rep2.me 29205228 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE104504/GSE104504.soft.gz 100 79 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: Mid exponential PGCGROWTHCONDITIONS SRR6125558 MCO000002864 exponential Growth phase True
characteristics exponential phase growth phase : <Phase> exponential </Phase> GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331409 LB 0.4 B1 TEX neg L1 GA 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 79 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: exponential PGCGROWTHCONDITIONS SRR1173965 MCO000002864 exponential Growth phase True
characteristics exponential phase growth phase : <Phase> exponential </Phase> GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331410 LB 0.4 B1 TEX pos L1 GA 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 79 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: exponential PGCGROWTHCONDITIONS SRR1173966 MCO000002864 exponential Growth phase True
characteristics exponential phase growth phase : <Phase> exponential </Phase> GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331411 LB 0.4 B2 TEX neg L1 HS1 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 79 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: exponential PGCGROWTHCONDITIONS SRR1173967 MCO000002864 exponential Growth phase True
characteristics exponential phase growth phase : <Phase> exponential </Phase> GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331412 LB 0.4 B2 TEX neg L1 HS2 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 79 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: exponential PGCGROWTHCONDITIONS SRR1173968 MCO000002864 exponential Growth phase True
characteristics exponential phase growth phase : <Phase> exponential </Phase> GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331413 LB 0.4 B2 TEX pos L1 HS1 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 79 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: exponential PGCGROWTHCONDITIONS SRR1173969 MCO000002864 exponential Growth phase True
characteristics exponential phase growth phase : <Phase> exponential </Phase> GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331414 LB 0.4 B2 TEX pos L1 HS2 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 79 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: exponential PGCGROWTHCONDITIONS SRR1173970 MCO000002864 exponential Growth phase True
characteristics exponential phase growth phase : <Phase> exponential </Phase> GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331426 M63 0.4 B1 TEX pos L1 GA 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 79 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: exponential PGCGROWTHCONDITIONS SRR1173982 MCO000002864 exponential Growth phase True
characteristics exponential phase growth phase : <Phase> exponential </Phase> GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331427 M63 0.4 B2 TEX neg L1 HS1 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 79 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: exponential PGCGROWTHCONDITIONS SRR1173983 MCO000002864 exponential Growth phase True
characteristics exponential phase growth phase : <Phase> exponential </Phase> GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331428 M63 0.4 B2 TEX neg L1 HS2 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 79 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: exponential PGCGROWTHCONDITIONS SRR1173984 MCO000002864 exponential Growth phase True
characteristics exponential phase growth phase : <Phase> exponential </Phase> GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331429 M63 0.4 B2 TEX pos L1 HS1 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 79 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: exponential PGCGROWTHCONDITIONS SRR1173985 MCO000002864 exponential Growth phase True
characteristics exponential phase growth phase : <Phase> exponential </Phase> GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331430 M63 0.4 B2 TEX pos L1 HS2 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 79 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: exponential PGCGROWTHCONDITIONS SRR1173986 MCO000002864 exponential Growth phase True
characteristics 30.0 C growth condition : <Supp> MM + 0.12 % casaminoacids +0.4 % glucose </Supp> at <Temp> 30 °C </Temp> GPL15010-GPL21117 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL21117: Illumina NextSeq 500 (Escherichia coli str. K-12 substr. MG1655) GSE107301 Multiscale structuring of the E. coli chromosome by nucleoid-associated and condensin proteins GSM2870440 RNA-seq of E. coli wt cells in stationary phase 30°C (30h) 29358050 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107301/GSE107301.soft.gz 100 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth condition: MM + 0.12% casaminoacids +0.4% glucose at 30°C PGCGROWTHCONDITIONS SRR6354578 MCO000002695 30 °C Temperature True
characteristics 30.0 C growth condition : <Supp> MM + 0.12 % casaminoacids +0.4 % glucose </Supp> at <Temp> 30 °C </Temp> GPL15010-GPL21117 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL21117: Illumina NextSeq 500 (Escherichia coli str. K-12 substr. MG1655) GSE107301 Multiscale structuring of the E. coli chromosome by nucleoid-associated and condensin proteins GSM2870441 RNA-seq of E. coli wt cells in stationary phase 30°C (30h) -Replicate 1 29358050 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE107301/GSE107301.soft.gz 100 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth condition: MM + 0.12% casaminoacids +0.4% glucose at 30°C PGCGROWTHCONDITIONS SRR6354579 MCO000002695 30 °C Temperature True
growth_protocol 30.0 C E. coli [ K-12 MG1655 strain ( <Gversion> U00096 .2 </Gversion> ) ] was grown overnight at <Temp> 30 °C </Temp> in LB medium . The resulting culture was diluted 500-fold in fresh LB medium and grown at 30 °C for 3.5 hours such that O.D. at 600 nm became 0.30-0.35 . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE34449 Digital RNA Sequencing Minimizes Sequence-Dependent Bias and Amplification Noise with Optimized Single Molecule Barcodes GSM849370 E_coli_transcriptome_1 22232676 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE34449/GSE34449.soft.gz 100 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli [K-12 MG1655 strain (U00096.2)] was grown overnight at 30 °C in LB medium. The resulting culture was diluted 500-fold in fresh LB medium and grown at 30 °C for 3.5 hours such that O.D. at 600 nm became 0.30-0.35. PGCGROWTHCONDITIONS SRR389819 MCO000002695 30 °C Temperature True
growth_protocol 30.0 C E. coli [ K-12 MG1655 strain ( <Gversion> U00096 .2 </Gversion> ) ] was grown overnight at <Temp> 30 °C </Temp> in LB medium . The resulting culture was diluted 500-fold in fresh LB medium and grown at 30 °C for 3.5 hours such that O.D. at 600 nm became 0.30-0.35 . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE34449 Digital RNA Sequencing Minimizes Sequence-Dependent Bias and Amplification Noise with Optimized Single Molecule Barcodes GSM849371 E_coli_transcriptome_2 22232676 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE34449/GSE34449.soft.gz 100 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli [K-12 MG1655 strain (U00096.2)] was grown overnight at 30 °C in LB medium. The resulting culture was diluted 500-fold in fresh LB medium and grown at 30 °C for 3.5 hours such that O.D. at 600 nm became 0.30-0.35. PGCGROWTHCONDITIONS SRR389820 MCO000002695 30 °C Temperature True
growth_protocol M9 minimal medium Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914313 CF104.3.3_y2 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449107 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914315 CF108.4B_y3 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449109 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914317 CON206.3A_y1 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449111 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914318 CON206.3A_y2 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449112 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914319 CON208.3A_y2 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449113 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914320 CON208.3A_y6 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449114 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914321 CF104.3.3_u1 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449115 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914322 CF104.3.3_u7 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449116 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914323 CF108.4B_u1 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449117 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914324 CF108.4B_u2 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449118 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914325 CON206.3A_u1 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449119 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914326 CON206.3A_u2 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449120 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914327 CON208.3A_u1 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449121 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium Experiments were performed at <Temp> 37 °C </Temp> and followed three steps : seed culture , pre-culture and experimental culture . In the seed culture the cells were grown overnight in Luria Broth , then diluted 1:100 in either <Med> M9 minimal media </Med> with glycerol or glucose for the pre-cultures . Finally , the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm ( OD600 ) of 0.05 . Cultures were harvested at mid-exponential phase , cells were immediately spun down and cell pellets stored at -80 °C until processed . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE108846 Adaptation of commensal proliferating Escherichia coli to the intestinal tract of young children with cystic fibrosis GSM2914328 CON208.3A_u8 29378945 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE108846/GSE108846.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Experiments were performed at 37°C and followed three steps: seed culture, pre-culture and experimental culture. In the seed culture the cells were grown overnight in Luria Broth, then diluted 1:100 in either M9 minimal media with glycerol or glucose for the pre-cultures. Finally, the experimental cultures were started from the overnight grown pre-cultures containing the same carbon source at a normalized optical density at 600 nm (OD600) of 0.05. Cultures were harvested at mid-exponential phase, cells were immediately spun down and cell pellets stored at -80°C until processed. PGCGROWTHCONDITIONS SRR6449122 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium glycerol stocks of E. coli strains were inoculated into 0.2 % ( w/v ) glucose M9 minimal media . <Med> M9 minimal media </Med> was also supplemented with 1 ml trace element solution ( 100X ) , excluding MG _ no _ te _ 1 and MG _ no _ te _ 2 . The culture was incubated at <Temp> 37 ℃ </Temp> overnight with agitation , and then was used to inoculate the fresh <Med> media </Med> . The fresh culture was incubated at 37 ℃ with agitation to the <Phase> mid-log phase </Phase> ( OD600 ≈ 0.5 ) . 10mM glutamate was added to the media MG _ glu _ 1 and MG _ glu _ 2 . GPL17024-GPL17439-GPL18995-GPL25769 GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655). GPL18995: Illumina MiSeq (Escherichia coli BW25113). GPL25769: Illumina Genome Analyzer IIx (Escherichia coli BW25113) GSE122211 Expression profiling of E. coli K-12 MG1655 GSM3461156 MG_no_te_1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122211/GSE122211.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt glycerol stocks of E. coli strains were inoculated into 0.2%(w/v) glucose M9 minimal media. M9 minimal media was also supplemented with 1 ml trace element solution (100X), excluding MG_no_te_1 and MG_no_te_2. The culture was incubated at 37 ℃ overnight with agitation, and then was used to inoculate the fresh media. The fresh culture was incubated at 37 ℃ with agitation to the mid-log phase (OD600 ≈ 0.5). 10mM glutamate was added to the media MG_glu_1 and MG_glu_2. PGCGROWTHCONDITIONS SRR8164476 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / 0.2 % glucose w/o trace elements GPL17024-GPL17439-GPL18995-GPL25769 GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655). GPL18995: Illumina MiSeq (Escherichia coli BW25113). GPL25769: Illumina Genome Analyzer IIx (Escherichia coli BW25113) GSE122211 Expression profiling of E. coli K-12 MG1655 GSM3461156 MG_no_te_1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122211/GSE122211.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 0.2% glucose w/o trace elements PGCGROWTHCONDITIONS SRR8164476 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium glycerol stocks of E. coli strains were inoculated into 0.2 % ( w/v ) glucose M9 minimal media . <Med> M9 minimal media </Med> was also supplemented with 1 ml trace element solution ( 100X ) , excluding MG _ no _ te _ 1 and MG _ no _ te _ 2 . The culture was incubated at <Temp> 37 ℃ </Temp> overnight with agitation , and then was used to inoculate the fresh <Med> media </Med> . The fresh culture was incubated at 37 ℃ with agitation to the <Phase> mid-log phase </Phase> ( OD600 ≈ 0.5 ) . 10mM glutamate was added to the media MG _ glu _ 1 and MG _ glu _ 2 . GPL17024-GPL17439-GPL18995-GPL25769 GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655). GPL18995: Illumina MiSeq (Escherichia coli BW25113). GPL25769: Illumina Genome Analyzer IIx (Escherichia coli BW25113) GSE122211 Expression profiling of E. coli K-12 MG1655 GSM3461157 MG_no_te_2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122211/GSE122211.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt glycerol stocks of E. coli strains were inoculated into 0.2%(w/v) glucose M9 minimal media. M9 minimal media was also supplemented with 1 ml trace element solution (100X), excluding MG_no_te_1 and MG_no_te_2. The culture was incubated at 37 ℃ overnight with agitation, and then was used to inoculate the fresh media. The fresh culture was incubated at 37 ℃ with agitation to the mid-log phase (OD600 ≈ 0.5). 10mM glutamate was added to the media MG_glu_1 and MG_glu_2. PGCGROWTHCONDITIONS SRR8164477 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / 0.2 % glucose w/o trace elements GPL17024-GPL17439-GPL18995-GPL25769 GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655). GPL18995: Illumina MiSeq (Escherichia coli BW25113). GPL25769: Illumina Genome Analyzer IIx (Escherichia coli BW25113) GSE122211 Expression profiling of E. coli K-12 MG1655 GSM3461157 MG_no_te_2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122211/GSE122211.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 0.2% glucose w/o trace elements PGCGROWTHCONDITIONS SRR8164477 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium glycerol stocks of E. coli strains were inoculated into 0.2 % ( w/v ) glucose M9 minimal media . <Med> M9 minimal media </Med> was also supplemented with 1 ml trace element solution ( 100X ) , excluding MG _ no _ te _ 1 and MG _ no _ te _ 2 . The culture was incubated at <Temp> 37 ℃ </Temp> overnight with agitation , and then was used to inoculate the fresh <Med> media </Med> . The fresh culture was incubated at 37 ℃ with agitation to the <Phase> mid-log phase </Phase> ( OD600 ≈ 0.5 ) . 10mM glutamate was added to the media MG _ glu _ 1 and MG _ glu _ 2 . GPL17024-GPL17439-GPL18995-GPL25769 GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655). GPL18995: Illumina MiSeq (Escherichia coli BW25113). GPL25769: Illumina Genome Analyzer IIx (Escherichia coli BW25113) GSE122211 Expression profiling of E. coli K-12 MG1655 GSM3461164 MG_glu_1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122211/GSE122211.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt glycerol stocks of E. coli strains were inoculated into 0.2%(w/v) glucose M9 minimal media. M9 minimal media was also supplemented with 1 ml trace element solution (100X), excluding MG_no_te_1 and MG_no_te_2. The culture was incubated at 37 ℃ overnight with agitation, and then was used to inoculate the fresh media. The fresh culture was incubated at 37 ℃ with agitation to the mid-log phase (OD600 ≈ 0.5). 10mM glutamate was added to the media MG_glu_1 and MG_glu_2. PGCGROWTHCONDITIONS SRR8164484 MCO000002526 M9 minimal media Medium True
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growth_protocol M9 minimal medium glycerol stocks of E. coli strains were inoculated into 0.2 % ( w/v ) glucose M9 minimal media . <Med> M9 minimal media </Med> was also supplemented with 1 ml trace element solution ( 100X ) , excluding MG _ no _ te _ 1 and MG _ no _ te _ 2 . The culture was incubated at <Temp> 37 ℃ </Temp> overnight with agitation , and then was used to inoculate the fresh <Med> media </Med> . The fresh culture was incubated at 37 ℃ with agitation to the <Phase> mid-log phase </Phase> ( OD600 ≈ 0.5 ) . 10mM glutamate was added to the media MG _ glu _ 1 and MG _ glu _ 2 . GPL17024-GPL17439-GPL18995-GPL25769 GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655). GPL18995: Illumina MiSeq (Escherichia coli BW25113). GPL25769: Illumina Genome Analyzer IIx (Escherichia coli BW25113) GSE122211 Expression profiling of E. coli K-12 MG1655 GSM3461165 MG_glu_2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122211/GSE122211.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt glycerol stocks of E. coli strains were inoculated into 0.2%(w/v) glucose M9 minimal media. M9 minimal media was also supplemented with 1 ml trace element solution (100X), excluding MG_no_te_1 and MG_no_te_2. The culture was incubated at 37 ℃ overnight with agitation, and then was used to inoculate the fresh media. The fresh culture was incubated at 37 ℃ with agitation to the mid-log phase (OD600 ≈ 0.5). 10mM glutamate was added to the media MG_glu_1 and MG_glu_2. PGCGROWTHCONDITIONS SRR8164485 MCO000002526 M9 minimal media Medium True
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growth_protocol M9 minimal medium glycerol stocks of E. coli strains were inoculated into 0.2 % ( w/v ) glucose M9 minimal media . <Med> M9 minimal media </Med> was also supplemented with 1 ml trace element solution ( 100X ) , excluding MG _ no _ te _ 1 and MG _ no _ te _ 2 . The culture was incubated at <Temp> 37 ℃ </Temp> overnight with agitation , and then was used to inoculate the fresh <Med> media </Med> . The fresh culture was incubated at 37 ℃ with agitation to the <Phase> mid-log phase </Phase> ( OD600 ≈ 0.5 ) . 10mM glutamate was added to the media MG _ glu _ 1 and MG _ glu _ 2 . GPL17024-GPL17439-GPL18995-GPL25769 GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655). GPL18995: Illumina MiSeq (Escherichia coli BW25113). GPL25769: Illumina Genome Analyzer IIx (Escherichia coli BW25113) GSE122211 Expression profiling of E. coli K-12 MG1655 GSM3461166 MG_glc 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122211/GSE122211.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt glycerol stocks of E. coli strains were inoculated into 0.2%(w/v) glucose M9 minimal media. M9 minimal media was also supplemented with 1 ml trace element solution (100X), excluding MG_no_te_1 and MG_no_te_2. The culture was incubated at 37 ℃ overnight with agitation, and then was used to inoculate the fresh media. The fresh culture was incubated at 37 ℃ with agitation to the mid-log phase (OD600 ≈ 0.5). 10mM glutamate was added to the media MG_glu_1 and MG_glu_2. PGCGROWTHCONDITIONS SRR8164486 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 0.2 % glucose </Supp> GPL17024-GPL17439-GPL18995-GPL25769 GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655). GPL18995: Illumina MiSeq (Escherichia coli BW25113). GPL25769: Illumina Genome Analyzer IIx (Escherichia coli BW25113) GSE122211 Expression profiling of E. coli K-12 MG1655 GSM3461166 MG_glc 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122211/GSE122211.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 0.2% glucose PGCGROWTHCONDITIONS SRR8164486 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463565 wt_glc__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR8173227 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463566 wt_glc__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR8173228 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463567 wt_glc__3 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR8173229 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463568 wt_glc__4 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR8173230 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / 4g/L D-sorbitol GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463569 arg_sbt__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L D-sorbitol PGCGROWTHCONDITIONS SRR8173231 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / 4g/L D-sorbitol GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463570 arg_sbt__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L D-sorbitol PGCGROWTHCONDITIONS SRR8173232 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / 4g/L D-ribose GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463571 cytd_rib__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L D-ribose PGCGROWTHCONDITIONS SRR8173233 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / 4g/L D-ribose GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463572 cytd_rib__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L D-ribose PGCGROWTHCONDITIONS SRR8173234 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463573 gth__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR8173235 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463574 gth__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR8173236 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / 4g/L glucarate GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463575 leu_glcr__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucarate PGCGROWTHCONDITIONS SRR8173237 MCO000002526 M9 minimal media Medium True
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characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463577 met_glc__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR8173239 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463578 met_glc__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR8173240 MCO000002526 M9 minimal media Medium True
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characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / 4g/L N-acetylglucosamine GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463581 phe_acgam__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L N-acetylglucosamine PGCGROWTHCONDITIONS SRR8173243 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / 4g/L N-acetylglucosamine GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463582 phe_acgam__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L N-acetylglucosamine PGCGROWTHCONDITIONS SRR8173244 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / 4g/L galactose GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463583 thm_gal__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L galactose PGCGROWTHCONDITIONS SRR8173245 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / 4g/L galactose GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463584 thm_gal__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L galactose PGCGROWTHCONDITIONS SRR8173246 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / 4g/L D-gluconate GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463585 tyr_glcn__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L D-gluconate PGCGROWTHCONDITIONS SRR8173247 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / 4g/L D-gluconate GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463586 tyr_glcn__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L D-gluconate PGCGROWTHCONDITIONS SRR8173248 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3854833 wt_glc_5__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR9204648 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3854834 wt_glc_6__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR9204649 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose and 1 mM cytidine </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3854835 bw_delpurR_cytd__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose and 1 mM cytidine PGCGROWTHCONDITIONS SRR9204650 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose and 1 mM cytidine </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3854836 bw_delpurR_cytd__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose and 1 mM cytidine PGCGROWTHCONDITIONS SRR9204651 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> and 100 mg/L adenine GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3854837 ade_glc__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose and 100 mg/L adenine PGCGROWTHCONDITIONS SRR9204652 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> and 100 mg/L adenine GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3854838 ade_glc__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose and 100 mg/L adenine PGCGROWTHCONDITIONS SRR9204653 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE122296 Expression profiling of multiple Escherichia coli strains on glucose minimal media GSM3463601 MG1655_1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122296/GSE122296.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR8173221 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE122296 Expression profiling of multiple Escherichia coli strains on glucose minimal media GSM3463602 MG1655_2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122296/GSE122296.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR8173222 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , Δfis and Δhns were grown in Luria-Bertani ( LB ) broth and agar ( 15g/l ) . Where needed , kanamycin was used at final concentration of 50µg/ml . For RNA extraction , E. coli cultures were grown to mid-exponential growth phase <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with 40 % ( w/v ) glucose . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE123554 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GSM3507068 delta-fis rep1 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE123554/GSE123554.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, Δfis and Δhns were grown in Luria-Bertani (LB) broth and agar (15g/l). Where needed, kanamycin was used at final concentration of 50µg/ml. For RNA extraction, E. coli cultures were grown to mid-exponential growth phase aerobically at 37°C in M9 minimal media supplemented with 40% (w/v) glucose. PGCGROWTHCONDITIONS SRR8309841 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , Δfis and Δhns were grown in Luria-Bertani ( LB ) broth and agar ( 15g/l ) . Where needed , kanamycin was used at final concentration of 50µg/ml . For RNA extraction , E. coli cultures were grown to mid-exponential growth phase <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with 40 % ( w/v ) glucose . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE123554 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GSM3507069 delta-fis rep2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE123554/GSE123554.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, Δfis and Δhns were grown in Luria-Bertani (LB) broth and agar (15g/l). Where needed, kanamycin was used at final concentration of 50µg/ml. For RNA extraction, E. coli cultures were grown to mid-exponential growth phase aerobically at 37°C in M9 minimal media supplemented with 40% (w/v) glucose. PGCGROWTHCONDITIONS SRR8309842 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , Δfis and Δhns were grown in Luria-Bertani ( LB ) broth and agar ( 15g/l ) . Where needed , kanamycin was used at final concentration of 50µg/ml . For RNA extraction , E. coli cultures were grown to mid-exponential growth phase <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with 40 % ( w/v ) glucose . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE123554 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GSM3507070 delta-hns rep1 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE123554/GSE123554.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, Δfis and Δhns were grown in Luria-Bertani (LB) broth and agar (15g/l). Where needed, kanamycin was used at final concentration of 50µg/ml. For RNA extraction, E. coli cultures were grown to mid-exponential growth phase aerobically at 37°C in M9 minimal media supplemented with 40% (w/v) glucose. PGCGROWTHCONDITIONS SRR8309843 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , Δfis and Δhns were grown in Luria-Bertani ( LB ) broth and agar ( 15g/l ) . Where needed , kanamycin was used at final concentration of 50µg/ml . For RNA extraction , E. coli cultures were grown to mid-exponential growth phase <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with 40 % ( w/v ) glucose . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE123554 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GSM3507071 delta-hns rep2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE123554/GSE123554.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, Δfis and Δhns were grown in Luria-Bertani (LB) broth and agar (15g/l). Where needed, kanamycin was used at final concentration of 50µg/ml. For RNA extraction, E. coli cultures were grown to mid-exponential growth phase aerobically at 37°C in M9 minimal media supplemented with 40% (w/v) glucose. PGCGROWTHCONDITIONS SRR8309844 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013462 WT_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919224 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013463 WT_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919225 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013464 WT_H2O2_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919226 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013465 WT_H2O2_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919227 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013466 GMOS_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919228 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013467 GMOS_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919229 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013468 GMOS_H2O2_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919230 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013470 EC ALE-1_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919232 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013471 EC ALE-1_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919233 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013472 EC ALE-1_H2O2_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919234 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013473 EC ALE-1_H2O2_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919235 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013474 EC ALE-2_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919236 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013475 EC ALE-2_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919237 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013476 EC ALE-2_H2O2_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919238 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013477 EC ALE-2_H2O2_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919239 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013478 EC ALE-3_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919240 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013479 EC ALE-3_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919241 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013480 EC ALE-3_H2O2_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919242 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013481 EC ALE-3_H2O2_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919243 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013482 EC ALE-4_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919244 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013483 EC ALE-4_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919245 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013484 EC ALE-4_H2O2_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919246 MCO000002526 M9 minimal media Medium True
characteristics M9 minimal medium media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013485 EC ALE-4_H2O2_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919247 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174823 Mid log_wildtype_glc minimal media_aerobic rep1 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922260 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174824 Mid log_wildtype_glc minimal media_aerobic rep2 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922261 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174825 Mid log_wildtype_glc minimal media_aerobic rep3 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922262 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174826 Mid log_wildtype_glc minimal media + adenine_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922263 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174827 Mid log_wildtype_glc minimal media + L-tryptophan_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922264 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174828 Mid log_wildtype_glc minimal media_anaerobic rep1 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922265 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174829 Mid log_wildtype_glc minimal media_anaerobic rep2 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922266 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174830 Mid log_wildtype_glc minimal media_anaerobic rep3 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922267 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174831 Mid log_nac KO_glc minimal media_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922268 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174832 Mid log_nac KO_glc minimal media + adenine_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922269 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174833 Mid log_cra KO_glc minimal media_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922270 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174834 Mid log_cra KO_glc minimal media + L-tryptophan_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922271 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174835 Mid log_mntR KO_glc minimal media_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922272 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174836 Mid log_mntR KO_glc minimal media_anaerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922273 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT and Δfur were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . For iron treated cells , 0.1 mM of FeCl2 were treated from the beginning of culture , and for DPD treated cells , <Supp> 0.2 mM of DPD </Supp> were added at early-log phase and continued to culture for additional <Supp> 2h </Supp> . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326347 WT with Fe 1 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. PGCGROWTHCONDITIONS SRR1168133 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT and Δfur were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . For iron treated cells , 0.1 mM of FeCl2 were treated from the beginning of culture , and for DPD treated cells , <Supp> 0.2 mM of DPD </Supp> were added at early-log phase and continued to culture for additional <Supp> 2h </Supp> . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326348 WT with Fe 2 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. PGCGROWTHCONDITIONS SRR1168134 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT and Δfur were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . For iron treated cells , 0.1 mM of FeCl2 were treated from the beginning of culture , and for DPD treated cells , <Supp> 0.2 mM of DPD </Supp> were added at early-log phase and continued to culture for additional <Supp> 2h </Supp> . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326349 WT with DPD 1 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. PGCGROWTHCONDITIONS SRR1168135 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT and Δfur were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . For iron treated cells , 0.1 mM of FeCl2 were treated from the beginning of culture , and for DPD treated cells , <Supp> 0.2 mM of DPD </Supp> were added at early-log phase and continued to culture for additional <Supp> 2h </Supp> . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326350 WT with DPD 2 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. PGCGROWTHCONDITIONS SRR1168136 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT and Δfur were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . For iron treated cells , 0.1 mM of FeCl2 were treated from the beginning of culture , and for DPD treated cells , <Supp> 0.2 mM of DPD </Supp> were added at early-log phase and continued to culture for additional <Supp> 2h </Supp> . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326351 Δfur with Fe 1 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. PGCGROWTHCONDITIONS SRR1168137 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT and Δfur were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . For iron treated cells , 0.1 mM of FeCl2 were treated from the beginning of culture , and for DPD treated cells , <Supp> 0.2 mM of DPD </Supp> were added at early-log phase and continued to culture for additional <Supp> 2h </Supp> . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326352 Δfur with Fe 2 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. PGCGROWTHCONDITIONS SRR1168138 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT and Δfur were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . For iron treated cells , 0.1 mM of FeCl2 were treated from the beginning of culture , and for DPD treated cells , <Supp> 0.2 mM of DPD </Supp> were added at early-log phase and continued to culture for additional <Supp> 2h </Supp> . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326353 Δfur with DPD 1 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. PGCGROWTHCONDITIONS SRR1168139 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT and Δfur were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . For iron treated cells , 0.1 mM of FeCl2 were treated from the beginning of culture , and for DPD treated cells , <Supp> 0.2 mM of DPD </Supp> were added at early-log phase and continued to culture for additional <Supp> 2h </Supp> . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326354 Δfur with DPD 2 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and Δfur were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. For iron treated cells, 0.1mM of FeCl2 were treated from the beginning of culture, and for DPD treated cells, 0.2mM of DPD were added at early-log phase and continued to culture for additional 2h. PGCGROWTHCONDITIONS SRR1168140 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602347 WT glucose 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787590 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602348 WT glucose 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787591 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602349 WT fructose 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787592 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602350 WT fructose 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787593 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602351 WT acetate 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787594 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602352 WT acetate 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787595 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602353 Δcra glucose 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787596 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602354 Δcra glucose 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787597 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602355 Δcra fructose 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787598 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602356 Δcra fructose 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787599 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602357 Δcra acetate 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787600 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , and Δcra were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> , fructose and acetate . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602358 Δcra acetate 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, and Δcra were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose, fructose and acetate. PGCGROWTHCONDITIONS SRR1787601 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , ΔoxyR , ΔsoxR , and ΔsoxS were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603386 WT PQ 1 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, ΔoxyR, ΔsoxR, and ΔsoxS were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation. PGCGROWTHCONDITIONS SRR1796598 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , ΔoxyR , ΔsoxR , and ΔsoxS were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603387 WT PQ 2 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, ΔoxyR, ΔsoxR, and ΔsoxS were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation. PGCGROWTHCONDITIONS SRR1796599 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , ΔoxyR , ΔsoxR , and ΔsoxS were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603388 ΔoxyR PQ 1 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, ΔoxyR, ΔsoxR, and ΔsoxS were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation. PGCGROWTHCONDITIONS SRR1796600 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , ΔoxyR , ΔsoxR , and ΔsoxS were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603389 ΔoxyR PQ 2 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, ΔoxyR, ΔsoxR, and ΔsoxS were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation. PGCGROWTHCONDITIONS SRR1796601 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , ΔoxyR , ΔsoxR , and ΔsoxS were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603391 ΔsoxR PQ 2 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, ΔoxyR, ΔsoxR, and ΔsoxS were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation. PGCGROWTHCONDITIONS SRR1796603 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , ΔoxyR , ΔsoxR , and ΔsoxS were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603392 ΔsoxS PQ 1 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, ΔoxyR, ΔsoxR, and ΔsoxS were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation. PGCGROWTHCONDITIONS SRR1796604 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , ΔoxyR , ΔsoxR , and ΔsoxS were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603393 ΔsoxS PQ 2 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, ΔoxyR, ΔsoxR, and ΔsoxS were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 250 uM of paraquat at mid-log pahse for 20 min with agitation. PGCGROWTHCONDITIONS SRR1796605 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623160 WT pH5.5 1 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824557 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623161 WT pH5.5 2 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824558 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623162 ΔgadE pH5.5 1 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824559 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623163 ΔgadE pH5.5 2 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824560 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623164 ΔgadW pH5.5 1 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824561 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623165 ΔgadW pH5.5 2 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824562 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623166 ΔgadX pH5.5 1 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824563 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623167 ΔgadX pH5.5 2 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824564 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium Escherichia coli strains E.coli C ( DSMZ 4860 ) , E. coli Crooks ( DSMZ 1576 ) , E. coli DH5α ( DSMZ 6897 ) E. coli W ( DSMZ 1116 ) , E. coli W3110 ( DSMZ 5911 ) were obtained from DSMZ-German Collection of Microorganism and Cell Cultures ; E. coli BL21 ( DE3 ) was purchased as competent cells from Agilent ( Agilent Technologies Inc. , USA ) , E. coli K-12 MG1655 ( ATCC 700926 ) . All strains were cultured in <Med> M9 minimal medium </Med> ( 1 ) containing Na2HPO4 x 7H2O ( 6.8 g ) , KH2PO4 ( 3 g ) , NaCl ( 0.5 g ) , NH4Cl ( 1 g ) , MgSO4 ( 2 mmol ) , CaCl2 ( 0.1 mmol ) , trace elements , Wolf 's vitamin solution ( 2 ) and glucose ( 2 g L-1 ) . Anoxic <Med> M9 minimal media </Med> with <Supp> glucose </Supp> was obtained by flushing solution with oxygen free nitrogen ( 95 % ) . Overnight cultures from single colonies of each of seven E. coli strains were diluted to a starting optical density ( OD600 ) of 0.01 . Cultures were grown in 250 ml flasks or 300 ml oxygen-free sealed bottles containing 50 ml <Med> glucose-M9 minimal media </Med> in a shaking incubator at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> . GPL14548-GPL15010-GPL21530-GPL21531-GPL21532-GPL21533-GPL21534 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL21530: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. W3110). GPL21531: Illumina HiSeq 2000 (Escherichia coli W). GPL21532: Illumina HiSeq 2000 (Escherichia coli C). GPL21533: Illumina HiSeq 2000 (Escherichia coli BL21). GPL21534: Illumina HiSeq 2000 (Escherichia coli DH5[alpha]) GSE78756 Quantifying variation within the bacterial species E. coli GSM2075722 Crooks_aero 27667363 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE78756/GSE78756.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Escherichia coli strains E.coli C (DSMZ 4860), E. coli Crooks (DSMZ 1576), E. coli DH5α (DSMZ 6897) E. coli W (DSMZ 1116), E. coli W3110 (DSMZ 5911) were obtained from DSMZ-German Collection of Microorganism and Cell Cultures; E. coli BL21 (DE3) was purchased as competent cells from Agilent (Agilent Technologies Inc., USA), E. coli K-12 MG1655 (ATCC 700926). All strains were cultured in M9 minimal medium (1) containing Na2HPO4 x 7H2O (6.8 g), KH2PO4 (3 g), NaCl (0.5 g), NH4Cl (1 g), MgSO4 (2 mmol), CaCl2 (0.1 mmol), trace elements, Wolf’s vitamin solution (2) and glucose (2 g L-1). Anoxic M9 minimal media with glucose was obtained by flushing solution with oxygen free nitrogen (95%). Overnight cultures from single colonies of each of seven E. coli strains were diluted to a starting optical density (OD600) of 0.01. Cultures were grown in 250 ml flasks or 300 ml oxygen-free sealed bottles containing 50 ml glucose-M9 minimal media in a shaking incubator at 37°C and 250 rpm. PGCGROWTHCONDITIONS SRR3194453 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium Escherichia coli strains E.coli C ( DSMZ 4860 ) , E. coli Crooks ( DSMZ 1576 ) , E. coli DH5α ( DSMZ 6897 ) E. coli W ( DSMZ 1116 ) , E. coli W3110 ( DSMZ 5911 ) were obtained from DSMZ-German Collection of Microorganism and Cell Cultures ; E. coli BL21 ( DE3 ) was purchased as competent cells from Agilent ( Agilent Technologies Inc. , USA ) , E. coli K-12 MG1655 ( ATCC 700926 ) . All strains were cultured in <Med> M9 minimal medium </Med> ( 1 ) containing Na2HPO4 x 7H2O ( 6.8 g ) , KH2PO4 ( 3 g ) , NaCl ( 0.5 g ) , NH4Cl ( 1 g ) , MgSO4 ( 2 mmol ) , CaCl2 ( 0.1 mmol ) , trace elements , Wolf 's vitamin solution ( 2 ) and glucose ( 2 g L-1 ) . Anoxic <Med> M9 minimal media </Med> with <Supp> glucose </Supp> was obtained by flushing solution with oxygen free nitrogen ( 95 % ) . Overnight cultures from single colonies of each of seven E. coli strains were diluted to a starting optical density ( OD600 ) of 0.01 . Cultures were grown in 250 ml flasks or 300 ml oxygen-free sealed bottles containing 50 ml <Med> glucose-M9 minimal media </Med> in a shaking incubator at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> . GPL14548-GPL15010-GPL21530-GPL21531-GPL21532-GPL21533-GPL21534 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL21530: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. W3110). GPL21531: Illumina HiSeq 2000 (Escherichia coli W). GPL21532: Illumina HiSeq 2000 (Escherichia coli C). GPL21533: Illumina HiSeq 2000 (Escherichia coli BL21). GPL21534: Illumina HiSeq 2000 (Escherichia coli DH5[alpha]) GSE78756 Quantifying variation within the bacterial species E. coli GSM2075723 Crooks_anaero 27667363 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE78756/GSE78756.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Escherichia coli strains E.coli C (DSMZ 4860), E. coli Crooks (DSMZ 1576), E. coli DH5α (DSMZ 6897) E. coli W (DSMZ 1116), E. coli W3110 (DSMZ 5911) were obtained from DSMZ-German Collection of Microorganism and Cell Cultures; E. coli BL21 (DE3) was purchased as competent cells from Agilent (Agilent Technologies Inc., USA), E. coli K-12 MG1655 (ATCC 700926). All strains were cultured in M9 minimal medium (1) containing Na2HPO4 x 7H2O (6.8 g), KH2PO4 (3 g), NaCl (0.5 g), NH4Cl (1 g), MgSO4 (2 mmol), CaCl2 (0.1 mmol), trace elements, Wolf’s vitamin solution (2) and glucose (2 g L-1). Anoxic M9 minimal media with glucose was obtained by flushing solution with oxygen free nitrogen (95%). Overnight cultures from single colonies of each of seven E. coli strains were diluted to a starting optical density (OD600) of 0.01. Cultures were grown in 250 ml flasks or 300 ml oxygen-free sealed bottles containing 50 ml glucose-M9 minimal media in a shaking incubator at 37°C and 250 rpm. PGCGROWTHCONDITIONS SRR3194455SRR3194456 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT and ΔompR were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with <Supp> 0.3 M of NaCl </Supp> at mid-log pahse for <Supp> 30 min </Supp> with agitation . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE88980 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GSM2356687 WT NaCl 1 28526842 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88980/GSE88980.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and ΔompR were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 0.3 M of NaCl at mid-log pahse for 30 min with agitation. PGCGROWTHCONDITIONS SRR4435464 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT and ΔompR were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with <Supp> 0.3 M of NaCl </Supp> at mid-log pahse for <Supp> 30 min </Supp> with agitation . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE88980 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GSM2356688 WT NaCl 2 28526842 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88980/GSE88980.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and ΔompR were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 0.3 M of NaCl at mid-log pahse for 30 min with agitation. PGCGROWTHCONDITIONS SRR4435465 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT and ΔompR were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with <Supp> 0.3 M of NaCl </Supp> at mid-log pahse for <Supp> 30 min </Supp> with agitation . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE88980 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GSM2356689 ΔompR NaCl 1 28526842 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88980/GSE88980.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and ΔompR were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 0.3 M of NaCl at mid-log pahse for 30 min with agitation. PGCGROWTHCONDITIONS SRR4435466 MCO000002526 M9 minimal media Medium True
growth_protocol M9 minimal medium E. coli K-12 MG1655 WT and ΔompR were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with <Supp> 0.3 M of NaCl </Supp> at mid-log pahse for <Supp> 30 min </Supp> with agitation . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE88980 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GSM2356690 ΔompR NaCl 2 28526842 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88980/GSE88980.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT and ΔompR were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 0.3 M of NaCl at mid-log pahse for 30 min with agitation. PGCGROWTHCONDITIONS SRR4435467 MCO000002526 M9 minimal media Medium True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3022135 WT rep1 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR6781997 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3022136 WT rep2 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR6781998 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3022137 yafC-KO rep1 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR6781999 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3022138 yafC-KO rep2 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR6782000 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3022139 yeiE-KO rep1 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR6782001 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3022140 yeiE-KO rep2 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR6782002 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3022141 yiaJ-KO rep1 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR6782003 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3022142 yiaJ-KO rep2 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR6782004 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3022143 yieP-KO rep1 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR6782005 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3022144 yieP-KO rep2 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR6782006 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3108934 WT-low-ph rep1 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR7057985 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3108935 WT-low-ph rep2 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR7057986 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3108936 WT-high-ph rep1 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR7057987 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3108937 WT-high-ph rep2 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR7057988 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3108938 ybaO-KO rep1 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR7057989 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3108939 ybaO-KO rep2 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR7057990 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3108940 ybaQ-KO rep1 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR7057991 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3108941 ybaQ-KO rep2 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR7057992 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3108942 ybiH-KO rep1 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR7057993 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3108943 ybiH-KO rep2 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR7057994 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3108944 ydcI-KO-low-ph rep1 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR7057995 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3108945 ydcI-KO-low-ph rep2 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR7057996 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3108946 ydcI-KO-high-ph rep1 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR7057997 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3108947 ydcI-KO-high-ph rep2 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR7057998 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3108948 yddM-KO rep1 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR7057999 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3108949 yddM-KO rep2 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR7058000 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3108950 yheO-KO rep1 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR7058001 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase phase : <Phase> mid-log phase </Phase> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3108951 yheO-KO rep2 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json phase: mid-log phase PGCGROWTHCONDITIONS SRR7058002 MCO000002872 mid-log phase Growth phase True
growth_protocol mid log phase glycerol stocks of E. coli strains were inoculated into M9 minimal media with <Supp> 2g/L glucose </Supp> , supplemented with 1 ml trace element solution ( 100X ) . The culture was incubated at 37C overnight with agitation , and then was used to inoculate the fresh <Med> media </Med> . The fresh culture was incubated at 37C with agitation to the <Phase> mid-log phase </Phase> ( OD600 ≈ 0.5 ) . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE122296 Expression profiling of multiple Escherichia coli strains on glucose minimal media GSM3463601 MG1655_1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122296/GSE122296.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json glycerol stocks of E. coli strains were inoculated into M9 minimal media with 2g/L glucose, supplemented with 1 ml trace element solution (100X). The culture was incubated at 37C overnight with agitation, and then was used to inoculate the fresh media. The fresh culture was incubated at 37C with agitation to the mid-log phase (OD600 ≈ 0.5). PGCGROWTHCONDITIONS SRR8173221 MCO000002872 mid-log phase Growth phase True
growth_protocol mid log phase glycerol stocks of E. coli strains were inoculated into M9 minimal media with <Supp> 2g/L glucose </Supp> , supplemented with 1 ml trace element solution ( 100X ) . The culture was incubated at 37C overnight with agitation , and then was used to inoculate the fresh <Med> media </Med> . The fresh culture was incubated at 37C with agitation to the <Phase> mid-log phase </Phase> ( OD600 ≈ 0.5 ) . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE122296 Expression profiling of multiple Escherichia coli strains on glucose minimal media GSM3463602 MG1655_2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122296/GSE122296.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json glycerol stocks of E. coli strains were inoculated into M9 minimal media with 2g/L glucose, supplemented with 1 ml trace element solution (100X). The culture was incubated at 37C overnight with agitation, and then was used to inoculate the fresh media. The fresh culture was incubated at 37C with agitation to the mid-log phase (OD600 ≈ 0.5). PGCGROWTHCONDITIONS SRR8173222 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase growth stage : <Phase> mid-log phase </Phase> GPL10328 GPL10328: Illumina Genome Analyzer II (Escherichia coli) GSE33671 Selective ribosome profiling reveals the co-translational chaperone action of trigger factor in vivo GSM832606 DSP1_AP 22153074 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE33671/GSE33671.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json growth stage: mid-log phase PGCGROWTHCONDITIONS SRR364363 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase growth stage : <Phase> mid-log phase </Phase> GPL10328 GPL10328: Illumina Genome Analyzer II (Escherichia coli) GSE33671 Selective ribosome profiling reveals the co-translational chaperone action of trigger factor in vivo GSM832607 DSP1_Total 22153074 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE33671/GSE33671.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json growth stage: mid-log phase PGCGROWTHCONDITIONS SRR364364 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase growth stage : <Phase> mid-log phase </Phase> GPL10328 GPL10328: Illumina Genome Analyzer II (Escherichia coli) GSE33671 Selective ribosome profiling reveals the co-translational chaperone action of trigger factor in vivo GSM832608 DSP2_AP 22153074 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE33671/GSE33671.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json growth stage: mid-log phase PGCGROWTHCONDITIONS SRR364365 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase growth stage : <Phase> mid-log phase </Phase> GPL10328 GPL10328: Illumina Genome Analyzer II (Escherichia coli) GSE33671 Selective ribosome profiling reveals the co-translational chaperone action of trigger factor in vivo GSM832609 DSP2_Total 22153074 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE33671/GSE33671.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json growth stage: mid-log phase PGCGROWTHCONDITIONS SRR364366 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase growth stage : <Phase> mid-log phase </Phase> GPL10328 GPL10328: Illumina Genome Analyzer II (Escherichia coli) GSE33671 Selective ribosome profiling reveals the co-translational chaperone action of trigger factor in vivo GSM832610 DSP3_AP 22153074 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE33671/GSE33671.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json growth stage: mid-log phase PGCGROWTHCONDITIONS SRR364367 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase growth stage : <Phase> mid-log phase </Phase> GPL10328 GPL10328: Illumina Genome Analyzer II (Escherichia coli) GSE33671 Selective ribosome profiling reveals the co-translational chaperone action of trigger factor in vivo GSM832611 DSP3_Total 22153074 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE33671/GSE33671.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json growth stage: mid-log phase PGCGROWTHCONDITIONS SRR364368 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase growth stage : <Phase> mid-log phase </Phase> GPL10328 GPL10328: Illumina Genome Analyzer II (Escherichia coli) GSE33671 Selective ribosome profiling reveals the co-translational chaperone action of trigger factor in vivo GSM832612 EDC1_AP 22153074 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE33671/GSE33671.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json growth stage: mid-log phase PGCGROWTHCONDITIONS SRR364369 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase growth stage : <Phase> mid-log phase </Phase> GPL10328 GPL10328: Illumina Genome Analyzer II (Escherichia coli) GSE33671 Selective ribosome profiling reveals the co-translational chaperone action of trigger factor in vivo GSM832613 EDC1_Total 22153074 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE33671/GSE33671.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json growth stage: mid-log phase PGCGROWTHCONDITIONS SRR364370 MCO000002872 mid-log phase Growth phase True
growth_protocol mid log phase E. coli MC4100 strain was cultured at <Temp> 37 °C </Temp> to <Phase> mid-log phase </Phase> ( OD600 ~ 0.4 ) in <Med> LB media </Med> GPL14548-GPL18945 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18945: Illumina Genome Analyzer IIx (Escherichia coli) GSE63817 Secondary structure across the bacterial transcriptome reveals versatile roles in mRNA regulation and function GSM1558078 LB mRNA 26495981 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE63817/GSE63817.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json E. coli MC4100 strain was cultured at 37°C to mid-log phase (OD600 ~ 0.4) in LB media PGCGROWTHCONDITIONS SRR1692165 MCO000002872 mid-log phase Growth phase True
growth_protocol mid log phase E. coli MC4100 strain was cultured at <Temp> 37 °C </Temp> to <Phase> mid-log phase </Phase> ( OD600 ~ 0.4 ) in <Med> LB media </Med> GPL14548-GPL18945 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18945: Illumina Genome Analyzer IIx (Escherichia coli) GSE63817 Secondary structure across the bacterial transcriptome reveals versatile roles in mRNA regulation and function GSM1558079 LB RPF 26495981 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE63817/GSE63817.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json E. coli MC4100 strain was cultured at 37°C to mid-log phase (OD600 ~ 0.4) in LB media PGCGROWTHCONDITIONS SRR1692166 MCO000002872 mid-log phase Growth phase True
growth_protocol mid log phase E. coli MC4100 strain was cultured at <Temp> 37 °C </Temp> to <Phase> mid-log phase </Phase> ( OD600 ~ 0.4 ) in <Med> LB media </Med> GPL14548-GPL18945 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18945: Illumina Genome Analyzer IIx (Escherichia coli) GSE63817 Secondary structure across the bacterial transcriptome reveals versatile roles in mRNA regulation and function GSM1558080 LB mRNA technical replicate 26495981 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE63817/GSE63817.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json E. coli MC4100 strain was cultured at 37°C to mid-log phase (OD600 ~ 0.4) in LB media PGCGROWTHCONDITIONS SRR1692167 MCO000002872 mid-log phase Growth phase True
growth_protocol mid log phase E. coli MC4100 strain was cultured at <Temp> 37 °C </Temp> to <Phase> mid-log phase </Phase> ( OD600 ~ 0.4 ) in <Med> LB media </Med> GPL14548-GPL18945 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18945: Illumina Genome Analyzer IIx (Escherichia coli) GSE63817 Secondary structure across the bacterial transcriptome reveals versatile roles in mRNA regulation and function GSM1558081 LB RPF biological replicate 26495981 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE63817/GSE63817.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json E. coli MC4100 strain was cultured at 37°C to mid-log phase (OD600 ~ 0.4) in LB media PGCGROWTHCONDITIONS SRR1692168 MCO000002872 mid-log phase Growth phase True
growth_protocol mid log phase E. coli MC4100 strain was cultured at <Temp> 37 °C </Temp> to <Phase> mid-log phase </Phase> ( OD600 ~ 0.4 ) in <Med> LB media </Med> GPL14548-GPL18945 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18945: Illumina Genome Analyzer IIx (Escherichia coli) GSE63817 Secondary structure across the bacterial transcriptome reveals versatile roles in mRNA regulation and function GSM1558082 LB mRNA biological replicate 26495981 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE63817/GSE63817.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json E. coli MC4100 strain was cultured at 37°C to mid-log phase (OD600 ~ 0.4) in LB media PGCGROWTHCONDITIONS SRR1692169 MCO000002872 mid-log phase Growth phase True
growth_protocol mid log phase E. coli MC4100 strain was cultured at <Temp> 37 °C </Temp> to <Phase> mid-log phase </Phase> ( OD600 ~ 0.4 ) in <Med> LB media </Med> GPL14548-GPL18945 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18945: Illumina Genome Analyzer IIx (Escherichia coli) GSE63817 Secondary structure across the bacterial transcriptome reveals versatile roles in mRNA regulation and function GSM1558086 AT1 biological replicate 26495981 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE63817/GSE63817.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json E. coli MC4100 strain was cultured at 37°C to mid-log phase (OD600 ~ 0.4) in LB media PGCGROWTHCONDITIONS SRR1692173 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase growth phase : <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623160 WT pH5.5 1 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json growth phase: mid-log phase (OD600 = 0.3) PGCGROWTHCONDITIONS SRR1824557 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase growth phase : <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623161 WT pH5.5 2 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json growth phase: mid-log phase (OD600 = 0.3) PGCGROWTHCONDITIONS SRR1824558 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase growth phase : <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623162 ΔgadE pH5.5 1 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json growth phase: mid-log phase (OD600 = 0.3) PGCGROWTHCONDITIONS SRR1824559 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase growth phase : <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623163 ΔgadE pH5.5 2 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json growth phase: mid-log phase (OD600 = 0.3) PGCGROWTHCONDITIONS SRR1824560 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase growth phase : <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623164 ΔgadW pH5.5 1 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json growth phase: mid-log phase (OD600 = 0.3) PGCGROWTHCONDITIONS SRR1824561 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase growth phase : <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623165 ΔgadW pH5.5 2 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json growth phase: mid-log phase (OD600 = 0.3) PGCGROWTHCONDITIONS SRR1824562 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase growth phase : <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623166 ΔgadX pH5.5 1 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json growth phase: mid-log phase (OD600 = 0.3) PGCGROWTHCONDITIONS SRR1824563 MCO000002872 mid-log phase Growth phase True
characteristics mid log phase growth phase : <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623167 ΔgadX pH5.5 2 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json growth phase: mid-log phase (OD600 = 0.3) PGCGROWTHCONDITIONS SRR1824564 MCO000002872 mid-log phase Growth phase True
data_processing Escherichia coli BW25113 Genome _ build : <Gtype> E.coli K12 BW25113 </Gtype> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE114262 RNA-seq Analyzes Transcriptomes of dnaB-Ts and dnaB-Ts ΔahpC at Both Permissive and Non-permissive Temperature GSM3138597 dnaB-Ts ΔahpC_30°C 30948634 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE114262/GSE114262.soft.gz 80 67 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Genome_build: E.coli K12 BW25113 PGCGROWTHCONDITIONS SRR7140738 MCO000002505 E.coli K12 BW25113 Genetic background True
data_processing Escherichia coli BW25113 Genome _ build : <Gtype> E.coli K12 BW25113 </Gtype> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE114262 RNA-seq Analyzes Transcriptomes of dnaB-Ts and dnaB-Ts ΔahpC at Both Permissive and Non-permissive Temperature GSM3138598 dnaB-Ts_42°C 30948634 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE114262/GSE114262.soft.gz 80 67 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Genome_build: E.coli K12 BW25113 PGCGROWTHCONDITIONS SRR7140739 MCO000002505 E.coli K12 BW25113 Genetic background True
data_processing Escherichia coli BW25113 Genome _ build : <Gtype> E.coli K12 BW25113 </Gtype> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE114262 RNA-seq Analyzes Transcriptomes of dnaB-Ts and dnaB-Ts ΔahpC at Both Permissive and Non-permissive Temperature GSM3138599 dnaB-Ts ΔahpC_42°C 30948634 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE114262/GSE114262.soft.gz 80 67 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Genome_build: E.coli K12 BW25113 PGCGROWTHCONDITIONS SRR7140740 MCO000002505 E.coli K12 BW25113 Genetic background True
growth_protocol synH medium Overnight cultures of the dnaB-Ts single mutant ( strain 2429 ) and the dnaB-Ts ΔahpC double mutant ( strain 3780 ) were diluted 100-fold into fresh <Med> SB medium </Med> ( 3.2 % peptone , 2 % yeast extract and 1 % NaCl ) , and they were then grown at 30 °C to early-log phase ( OD600 = 0.15 ) . Next these log-phase cultures were diluted 20-fold into 200 ml pre-warmed , fresh SB medium , and they were grown at 30 °C for another 60 min . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE114262 RNA-seq Analyzes Transcriptomes of dnaB-Ts and dnaB-Ts ΔahpC at Both Permissive and Non-permissive Temperature GSM3138597 dnaB-Ts ΔahpC_30°C 30948634 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE114262/GSE114262.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Overnight cultures of the dnaB-Ts single mutant (strain 2429) and the dnaB-Ts ΔahpC double mutant (strain 3780) were diluted 100-fold into fresh SB medium (3.2% peptone, 2% yeast extract and 1% NaCl), and they were then grown at 30 °C to early-log phase (OD600 = 0.15). Next these log-phase cultures were diluted 20-fold into 200 ml pre-warmed, fresh SB medium, and they were grown at 30 °C for another 60 min. PGCGROWTHCONDITIONS SRR7140738 MCO000003163 SB medium Medium True
growth_protocol synH medium Overnight cultures of the dnaB-Ts single mutant ( strain 2429 ) and the dnaB-Ts ΔahpC double mutant ( strain 3780 ) were diluted 100-fold into fresh <Med> SB medium </Med> ( 3.2 % peptone , 2 % yeast extract and 1 % NaCl ) , and they were then grown at 30 °C to early-log phase ( OD600 = 0.15 ) . Next these log-phase cultures were diluted 20-fold into 200 ml pre-warmed , fresh SB medium , and they were grown at 30 °C for another 60 min . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE114262 RNA-seq Analyzes Transcriptomes of dnaB-Ts and dnaB-Ts ΔahpC at Both Permissive and Non-permissive Temperature GSM3138598 dnaB-Ts_42°C 30948634 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE114262/GSE114262.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Overnight cultures of the dnaB-Ts single mutant (strain 2429) and the dnaB-Ts ΔahpC double mutant (strain 3780) were diluted 100-fold into fresh SB medium (3.2% peptone, 2% yeast extract and 1% NaCl), and they were then grown at 30 °C to early-log phase (OD600 = 0.15). Next these log-phase cultures were diluted 20-fold into 200 ml pre-warmed, fresh SB medium, and they were grown at 30 °C for another 60 min. PGCGROWTHCONDITIONS SRR7140739 MCO000003163 SB medium Medium True
growth_protocol synH medium Overnight cultures of the dnaB-Ts single mutant ( strain 2429 ) and the dnaB-Ts ΔahpC double mutant ( strain 3780 ) were diluted 100-fold into fresh <Med> SB medium </Med> ( 3.2 % peptone , 2 % yeast extract and 1 % NaCl ) , and they were then grown at 30 °C to early-log phase ( OD600 = 0.15 ) . Next these log-phase cultures were diluted 20-fold into 200 ml pre-warmed , fresh SB medium , and they were grown at 30 °C for another 60 min . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE114262 RNA-seq Analyzes Transcriptomes of dnaB-Ts and dnaB-Ts ΔahpC at Both Permissive and Non-permissive Temperature GSM3138599 dnaB-Ts ΔahpC_42°C 30948634 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE114262/GSE114262.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Overnight cultures of the dnaB-Ts single mutant (strain 2429) and the dnaB-Ts ΔahpC double mutant (strain 3780) were diluted 100-fold into fresh SB medium (3.2% peptone, 2% yeast extract and 1% NaCl), and they were then grown at 30 °C to early-log phase (OD600 = 0.15). Next these log-phase cultures were diluted 20-fold into 200 ml pre-warmed, fresh SB medium, and they were grown at 30 °C for another 60 min. PGCGROWTHCONDITIONS SRR7140740 MCO000003163 SB medium Medium True
characteristics wild type plasmid : <Gtype> pLCenvZ , pPCB ( wild type ) </Gtype> GPL25346 GPL25346: HiSeq X Ten (Escherichia coli) GSE117326 Gene expression profiles in E. coli under different frequency signals GSM3291044 envz600 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117326/GSE117326.soft.gz 100 58 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt plasmid: pLCenvZ, pPCB (wild type) PGCGROWTHCONDITIONS SRR7537397 MCO000002470 pLCenvZ , pPCB ( wild type ) Genetic background True
characteristics wild type plasmid : <Gtype> pLCenvZ , pPCB ( wild type ) </Gtype> GPL25346 GPL25346: HiSeq X Ten (Escherichia coli) GSE117326 Gene expression profiles in E. coli under different frequency signals GSM3291045 envz900 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117326/GSE117326.soft.gz 100 58 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt plasmid: pLCenvZ, pPCB (wild type) PGCGROWTHCONDITIONS SRR7537398 MCO000002470 pLCenvZ , pPCB ( wild type ) Genetic background True
characteristics wild type plasmid : <Gtype> pLCenvZ , pPCB ( wild type ) </Gtype> GPL25346 GPL25346: HiSeq X Ten (Escherichia coli) GSE117326 Gene expression profiles in E. coli under different frequency signals GSM3291049 envz3600 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117326/GSE117326.soft.gz 100 58 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt plasmid: pLCenvZ, pPCB (wild type) PGCGROWTHCONDITIONS SRR7537402 MCO000002470 pLCenvZ , pPCB ( wild type ) Genetic background True
growth_protocol LB medium Escherichia coli K-12 MG1655 were cultured in <Med> LB media </Med> ( 10 g/L tryptone , 5 g/L yeast extract , 10 g/L NaCl , pH 7.4 ) under aerobic conditions at <Temp> 37 °C </Temp> . The temperature-sensitive RNase E mutant E.coli strain [ rne-3071 ( ts ) ] was cultured in <Med> LB media </Med> at 28 °C for normal culture and shift to 44 °C to deactive RNase E activity . GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566393 RNA-seq WT log phase related to total SEnd-seq WT log phase rep 4 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 82 82 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Escherichia coli K-12 MG1655 were cultured in LB media (10 g/L tryptone, 5 g/L yeast extract, 10 g/L NaCl, pH 7.4) under aerobic conditions at 37 °C. The temperature-sensitive RNase E mutant E.coli strain [rne-3071(ts)] was cultured in LB media at 28 °C for normal culture and shift to 44 °C to deactive RNase E activity. PGCGROWTHCONDITIONS SRR8449235 MCO000002533 LB media Medium True
treatment_protocol LB medium <Gtype> Wild type </Gtype> E. coli , pnp knoctout , rnb knoctout and rnr knoct out strains were cultured in <Med> LB media </Med> at <Temp> 37 °C </Temp> , RNA samples and ChIP-seq samples were collected when the growth stage of indicated cells reached to log phase or stationary phase . rne-3071 ( ts ) mutation strain was cultured in <Med> LB media </Med> at 28 °C to retain the RNase E activity or shifted to 44 °C in water bath for 30 minutes to deactivate RNase E . In order to <Gtype> inactive the rho activity , the wild type ( WT ) Wild type </Gtype> E. coli was cultured in <Med> LB media </Med> with 50 μg/ml bicyclomycin at <Temp> 37 °C </Temp> for 15 min at indicated growth condition . GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566393 RNA-seq WT log phase related to total SEnd-seq WT log phase rep 4 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 82 82 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Wild type E. coli, pnp knoctout, rnb knoctout and rnr knoct out strains were cultured in LB media at 37 °C, RNA samples and ChIP-seq samples were collected when the growth stage of indicated cells reached to log phase or stationary phase. rne-3071(ts) mutation strain was cultured in LB media at 28 °C to retain the RNase E activity or shifted to 44 °C in water bath for 30 minutes to deactivate RNase E. In order to inactive the rho activity, the wild type (WT) Wild type E. coli was cultured in LB media with 50 μg/ml bicyclomycin at 37 °C for 15 min at indicated growth condition. PGCGROWTHCONDITIONS SRR8449235 MCO000002533 LB media Medium True
growth_protocol LB medium Escherichia coli K-12 MG1655 were cultured in <Med> LB media </Med> ( 10 g/L tryptone , 5 g/L yeast extract , 10 g/L NaCl , pH 7.4 ) under aerobic conditions at <Temp> 37 °C </Temp> . The temperature-sensitive RNase E mutant E.coli strain [ rne-3071 ( ts ) ] was cultured in <Med> LB media </Med> at 28 °C for normal culture and shift to 44 °C to deactive RNase E activity . GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566394 RNA-seq WT log phase related to total SEnd-seq WT log phase rep 5 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 82 82 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Escherichia coli K-12 MG1655 were cultured in LB media (10 g/L tryptone, 5 g/L yeast extract, 10 g/L NaCl, pH 7.4) under aerobic conditions at 37 °C. The temperature-sensitive RNase E mutant E.coli strain [rne-3071(ts)] was cultured in LB media at 28 °C for normal culture and shift to 44 °C to deactive RNase E activity. PGCGROWTHCONDITIONS SRR8449236 MCO000002533 LB media Medium True
treatment_protocol LB medium <Gtype> Wild type </Gtype> E. coli , pnp knoctout , rnb knoctout and rnr knoct out strains were cultured in <Med> LB media </Med> at <Temp> 37 °C </Temp> , RNA samples and ChIP-seq samples were collected when the growth stage of indicated cells reached to log phase or stationary phase . rne-3071 ( ts ) mutation strain was cultured in <Med> LB media </Med> at 28 °C to retain the RNase E activity or shifted to 44 °C in water bath for 30 minutes to deactivate RNase E . In order to <Gtype> inactive the rho activity , the wild type ( WT ) Wild type </Gtype> E. coli was cultured in <Med> LB media </Med> with 50 μg/ml bicyclomycin at <Temp> 37 °C </Temp> for 15 min at indicated growth condition . GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566394 RNA-seq WT log phase related to total SEnd-seq WT log phase rep 5 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 82 82 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Wild type E. coli, pnp knoctout, rnb knoctout and rnr knoct out strains were cultured in LB media at 37 °C, RNA samples and ChIP-seq samples were collected when the growth stage of indicated cells reached to log phase or stationary phase. rne-3071(ts) mutation strain was cultured in LB media at 28 °C to retain the RNase E activity or shifted to 44 °C in water bath for 30 minutes to deactivate RNase E. In order to inactive the rho activity, the wild type (WT) Wild type E. coli was cultured in LB media with 50 μg/ml bicyclomycin at 37 °C for 15 min at indicated growth condition. PGCGROWTHCONDITIONS SRR8449236 MCO000002533 LB media Medium True
growth_protocol LB medium Escherichia coli K-12 MG1655 were cultured in <Med> LB media </Med> ( 10 g/L tryptone , 5 g/L yeast extract , 10 g/L NaCl , pH 7.4 ) under aerobic conditions at <Temp> 37 °C </Temp> . The temperature-sensitive RNase E mutant E.coli strain [ rne-3071 ( ts ) ] was cultured in <Med> LB media </Med> at 28 °C for normal culture and shift to 44 °C to deactive RNase E activity . GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566395 RNA-seq WT log phase related to total SEnd-seq WT log phase rep 6 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 82 82 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Escherichia coli K-12 MG1655 were cultured in LB media (10 g/L tryptone, 5 g/L yeast extract, 10 g/L NaCl, pH 7.4) under aerobic conditions at 37 °C. The temperature-sensitive RNase E mutant E.coli strain [rne-3071(ts)] was cultured in LB media at 28 °C for normal culture and shift to 44 °C to deactive RNase E activity. PGCGROWTHCONDITIONS SRR8449237 MCO000002533 LB media Medium True
treatment_protocol LB medium <Gtype> Wild type </Gtype> E. coli , pnp knoctout , rnb knoctout and rnr knoct out strains were cultured in <Med> LB media </Med> at <Temp> 37 °C </Temp> , RNA samples and ChIP-seq samples were collected when the growth stage of indicated cells reached to log phase or stationary phase . rne-3071 ( ts ) mutation strain was cultured in <Med> LB media </Med> at 28 °C to retain the RNase E activity or shifted to 44 °C in water bath for 30 minutes to deactivate RNase E . In order to <Gtype> inactive the rho activity , the wild type ( WT ) Wild type </Gtype> E. coli was cultured in <Med> LB media </Med> with 50 μg/ml bicyclomycin at <Temp> 37 °C </Temp> for 15 min at indicated growth condition . GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566395 RNA-seq WT log phase related to total SEnd-seq WT log phase rep 6 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 82 82 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Wild type E. coli, pnp knoctout, rnb knoctout and rnr knoct out strains were cultured in LB media at 37 °C, RNA samples and ChIP-seq samples were collected when the growth stage of indicated cells reached to log phase or stationary phase. rne-3071(ts) mutation strain was cultured in LB media at 28 °C to retain the RNase E activity or shifted to 44 °C in water bath for 30 minutes to deactivate RNase E. In order to inactive the rho activity, the wild type (WT) Wild type E. coli was cultured in LB media with 50 μg/ml bicyclomycin at 37 °C for 15 min at indicated growth condition. PGCGROWTHCONDITIONS SRR8449237 MCO000002533 LB media Medium True
growth_protocol LB medium Escherichia coli K-12 MG1655 were cultured in <Med> LB media </Med> ( 10 g/L tryptone , 5 g/L yeast extract , 10 g/L NaCl , pH 7.4 ) under aerobic conditions at <Temp> 37 °C </Temp> . The temperature-sensitive RNase E mutant E.coli strain [ rne-3071 ( ts ) ] was cultured in <Med> LB media </Med> at 28 °C for normal culture and shift to 44 °C to deactive RNase E activity . GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566396 RNA-seq WT log phase related to total SEnd-seq WT stationary phase rep4 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 82 82 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Escherichia coli K-12 MG1655 were cultured in LB media (10 g/L tryptone, 5 g/L yeast extract, 10 g/L NaCl, pH 7.4) under aerobic conditions at 37 °C. The temperature-sensitive RNase E mutant E.coli strain [rne-3071(ts)] was cultured in LB media at 28 °C for normal culture and shift to 44 °C to deactive RNase E activity. PGCGROWTHCONDITIONS SRR8449238 MCO000002533 LB media Medium True
treatment_protocol LB medium <Gtype> Wild type </Gtype> E. coli , pnp knoctout , rnb knoctout and rnr knoct out strains were cultured in <Med> LB media </Med> at <Temp> 37 °C </Temp> , RNA samples and ChIP-seq samples were collected when the growth stage of indicated cells reached to log phase or stationary phase . rne-3071 ( ts ) mutation strain was cultured in <Med> LB media </Med> at 28 °C to retain the RNase E activity or shifted to 44 °C in water bath for 30 minutes to deactivate RNase E . In order to <Gtype> inactive the rho activity , the wild type ( WT ) Wild type </Gtype> E. coli was cultured in <Med> LB media </Med> with 50 μg/ml bicyclomycin at <Temp> 37 °C </Temp> for 15 min at indicated growth condition . GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566396 RNA-seq WT log phase related to total SEnd-seq WT stationary phase rep4 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 82 82 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Wild type E. coli, pnp knoctout, rnb knoctout and rnr knoct out strains were cultured in LB media at 37 °C, RNA samples and ChIP-seq samples were collected when the growth stage of indicated cells reached to log phase or stationary phase. rne-3071(ts) mutation strain was cultured in LB media at 28 °C to retain the RNase E activity or shifted to 44 °C in water bath for 30 minutes to deactivate RNase E. In order to inactive the rho activity, the wild type (WT) Wild type E. coli was cultured in LB media with 50 μg/ml bicyclomycin at 37 °C for 15 min at indicated growth condition. PGCGROWTHCONDITIONS SRR8449238 MCO000002533 LB media Medium True
extract_protocol LB medium From the cells cultured in <Med> LB media </Med> at 37 ℃ , total RNA was extracted using RNAsnapTM method , followed by the ethanol precipitation . rRNA was removed using ribo-zeroTM magnetic kit for bacteria in accordance with manufacturer 's instruction ( Epicentre ) . rRNA removal was confirmed using ExperionTM system . Subsequently , 4 µg of the purified RNA was fragmented to sizes of ~ 300 bp using RNA fragmentation reagent ( Ambion , Grand Island , NY ) . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE67218 Transcriptional expression level of E. coli at 37 ℃ in LB media GSM1642593 RNA-seq 37C LB rep1 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE67218/GSE67218.soft.gz 82 82 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt From the cells cultured in LB media at 37 ℃, total RNA was extracted using RNAsnapTM method, followed by the ethanol precipitation. rRNA was removed using ribo-zeroTM magnetic kit for bacteria in accordance with manufacturer’s instruction (Epicentre). rRNA removal was confirmed using ExperionTM system. Subsequently, 4 µg of the purified RNA was fragmented to sizes of ~300 bp using RNA fragmentation reagent (Ambion, Grand Island, NY). PGCGROWTHCONDITIONS SRR1927169 MCO000002533 LB media Medium True
extract_protocol LB medium From the cells cultured in <Med> LB media </Med> at 37 ℃ , total RNA was extracted using RNAsnapTM method , followed by the ethanol precipitation . rRNA was removed using ribo-zeroTM magnetic kit for bacteria in accordance with manufacturer 's instruction ( Epicentre ) . rRNA removal was confirmed using ExperionTM system . Subsequently , 4 µg of the purified RNA was fragmented to sizes of ~ 300 bp using RNA fragmentation reagent ( Ambion , Grand Island , NY ) . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE67218 Transcriptional expression level of E. coli at 37 ℃ in LB media GSM1642594 RNA-seq 37C LB rep2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE67218/GSE67218.soft.gz 82 82 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt From the cells cultured in LB media at 37 ℃, total RNA was extracted using RNAsnapTM method, followed by the ethanol precipitation. rRNA was removed using ribo-zeroTM magnetic kit for bacteria in accordance with manufacturer’s instruction (Epicentre). rRNA removal was confirmed using ExperionTM system. Subsequently, 4 µg of the purified RNA was fragmented to sizes of ~300 bp using RNA fragmentation reagent (Ambion, Grand Island, NY). PGCGROWTHCONDITIONS SRR1927170 MCO000002533 LB media Medium True
treatment_protocol wild type <Gtype> Wild type </Gtype> E. coli , pnp knoctout , rnb knoctout and rnr knoct out strains were cultured in <Med> LB media </Med> at <Temp> 37 °C </Temp> , RNA samples and ChIP-seq samples were collected when the growth stage of indicated cells reached to log phase or stationary phase . rne-3071 ( ts ) mutation strain was cultured in <Med> LB media </Med> at 28 °C to retain the RNase E activity or shifted to 44 °C in water bath for 30 minutes to deactivate RNase E . In order to <Gtype> inactive the rho activity , the wild type ( WT ) Wild type </Gtype> E. coli was cultured in <Med> LB media </Med> with 50 μg/ml bicyclomycin at <Temp> 37 °C </Temp> for 15 min at indicated growth condition . GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566393 RNA-seq WT log phase related to total SEnd-seq WT log phase rep 4 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Wild type E. coli, pnp knoctout, rnb knoctout and rnr knoct out strains were cultured in LB media at 37 °C, RNA samples and ChIP-seq samples were collected when the growth stage of indicated cells reached to log phase or stationary phase. rne-3071(ts) mutation strain was cultured in LB media at 28 °C to retain the RNase E activity or shifted to 44 °C in water bath for 30 minutes to deactivate RNase E. In order to inactive the rho activity, the wild type (WT) Wild type E. coli was cultured in LB media with 50 μg/ml bicyclomycin at 37 °C for 15 min at indicated growth condition. PGCGROWTHCONDITIONS SRR8449235 MCO000002470 Wild type Genetic background True
treatment_protocol wild type <Gtype> Wild type </Gtype> E. coli , pnp knoctout , rnb knoctout and rnr knoct out strains were cultured in <Med> LB media </Med> at <Temp> 37 °C </Temp> , RNA samples and ChIP-seq samples were collected when the growth stage of indicated cells reached to log phase or stationary phase . rne-3071 ( ts ) mutation strain was cultured in <Med> LB media </Med> at 28 °C to retain the RNase E activity or shifted to 44 °C in water bath for 30 minutes to deactivate RNase E . In order to <Gtype> inactive the rho activity , the wild type ( WT ) Wild type </Gtype> E. coli was cultured in <Med> LB media </Med> with 50 μg/ml bicyclomycin at <Temp> 37 °C </Temp> for 15 min at indicated growth condition . GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566394 RNA-seq WT log phase related to total SEnd-seq WT log phase rep 5 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Wild type E. coli, pnp knoctout, rnb knoctout and rnr knoct out strains were cultured in LB media at 37 °C, RNA samples and ChIP-seq samples were collected when the growth stage of indicated cells reached to log phase or stationary phase. rne-3071(ts) mutation strain was cultured in LB media at 28 °C to retain the RNase E activity or shifted to 44 °C in water bath for 30 minutes to deactivate RNase E. In order to inactive the rho activity, the wild type (WT) Wild type E. coli was cultured in LB media with 50 μg/ml bicyclomycin at 37 °C for 15 min at indicated growth condition. PGCGROWTHCONDITIONS SRR8449236 MCO000002470 Wild type Genetic background True
treatment_protocol wild type <Gtype> Wild type </Gtype> E. coli , pnp knoctout , rnb knoctout and rnr knoct out strains were cultured in <Med> LB media </Med> at <Temp> 37 °C </Temp> , RNA samples and ChIP-seq samples were collected when the growth stage of indicated cells reached to log phase or stationary phase . rne-3071 ( ts ) mutation strain was cultured in <Med> LB media </Med> at 28 °C to retain the RNase E activity or shifted to 44 °C in water bath for 30 minutes to deactivate RNase E . In order to <Gtype> inactive the rho activity , the wild type ( WT ) Wild type </Gtype> E. coli was cultured in <Med> LB media </Med> with 50 μg/ml bicyclomycin at <Temp> 37 °C </Temp> for 15 min at indicated growth condition . GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566395 RNA-seq WT log phase related to total SEnd-seq WT log phase rep 6 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Wild type E. coli, pnp knoctout, rnb knoctout and rnr knoct out strains were cultured in LB media at 37 °C, RNA samples and ChIP-seq samples were collected when the growth stage of indicated cells reached to log phase or stationary phase. rne-3071(ts) mutation strain was cultured in LB media at 28 °C to retain the RNase E activity or shifted to 44 °C in water bath for 30 minutes to deactivate RNase E. In order to inactive the rho activity, the wild type (WT) Wild type E. coli was cultured in LB media with 50 μg/ml bicyclomycin at 37 °C for 15 min at indicated growth condition. PGCGROWTHCONDITIONS SRR8449237 MCO000002470 Wild type Genetic background True
treatment_protocol wild type <Gtype> Wild type </Gtype> E. coli , pnp knoctout , rnb knoctout and rnr knoct out strains were cultured in <Med> LB media </Med> at <Temp> 37 °C </Temp> , RNA samples and ChIP-seq samples were collected when the growth stage of indicated cells reached to log phase or stationary phase . rne-3071 ( ts ) mutation strain was cultured in <Med> LB media </Med> at 28 °C to retain the RNase E activity or shifted to 44 °C in water bath for 30 minutes to deactivate RNase E . In order to <Gtype> inactive the rho activity , the wild type ( WT ) Wild type </Gtype> E. coli was cultured in <Med> LB media </Med> with 50 μg/ml bicyclomycin at <Temp> 37 °C </Temp> for 15 min at indicated growth condition . GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566396 RNA-seq WT log phase related to total SEnd-seq WT stationary phase rep4 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Wild type E. coli, pnp knoctout, rnb knoctout and rnr knoct out strains were cultured in LB media at 37 °C, RNA samples and ChIP-seq samples were collected when the growth stage of indicated cells reached to log phase or stationary phase. rne-3071(ts) mutation strain was cultured in LB media at 28 °C to retain the RNase E activity or shifted to 44 °C in water bath for 30 minutes to deactivate RNase E. In order to inactive the rho activity, the wild type (WT) Wild type E. coli was cultured in LB media with 50 μg/ml bicyclomycin at 37 °C for 15 min at indicated growth condition. PGCGROWTHCONDITIONS SRR8449238 MCO000002470 Wild type Genetic background True
growth_protocol wild type <Gtype> Wild type </Gtype> E. coli K-12 ( strain BW38038 ) and BW39452 ( ΔrpoS ) cultures were grown on <Med> MOPS glucose minimal medium </Med> with <Supp> 0.2 % glucose </Supp> as sole carbon source at <Temp> 37 °C </Temp> , pH was initially 7.4 , and the agitation speed was 500 rpm . Culture samples were harvested during logarithmic growth and following entry into stationary phase for the WT and rpoS mutant . OD600 measurements were made on a Beckman Coulter DU 800 spectrophotometer . Samples were harvested directly into ice-cold RNAlater at a 1:1 dilution to protect RNA from degradation and cells then were pelleted by centrifugation at 8000rpm for 10 minutes . Cell pellets were stored at -80 °C in an equal volume of RNAlater prior to RNA extraction . GPL18814 GPL18814: Illumina HiSeq 2000 (Escherichia coli BW38028) GSE58556 RNA sequencing based analysis of the bacterial transcriptome GSM1413874 WT_glucose_log 25483350 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE58556/GSE58556.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Wild type E. coli K-12 (strain BW38038) and BW39452(ΔrpoS) cultures were grown on MOPS glucose minimal medium with 0.2% glucose as sole carbon source at 37°C, pH was initially 7.4, and the agitation speed was 500 rpm. Culture samples were harvested during logarithmic growth and following entry into stationary phase for the WT and rpoS mutant. OD600 measurements were made on a Beckman Coulter DU 800 spectrophotometer. Samples were harvested directly into ice-cold RNAlater at a 1:1 dilution to protect RNA from degradation and cells then were pelleted by centrifugation at 8000rpm for 10 minutes. Cell pellets were stored at -80°C in an equal volume of RNAlater prior to RNA extraction. PGCGROWTHCONDITIONS SRR1411272 MCO000002470 Wild type Genetic background True
growth_protocol 37.0 C glycerol stocks of E. coli strains were inoculated into 0.2 % ( w/v ) glucose M9 minimal media . <Med> M9 minimal media </Med> was also supplemented with 1 ml trace element solution ( 100X ) , excluding MG _ no _ te _ 1 and MG _ no _ te _ 2 . The culture was incubated at <Temp> 37 ℃ </Temp> overnight with agitation , and then was used to inoculate the fresh <Med> media </Med> . The fresh culture was incubated at 37 ℃ with agitation to the <Phase> mid-log phase </Phase> ( OD600 ≈ 0.5 ) . 10mM glutamate was added to the media MG _ glu _ 1 and MG _ glu _ 2 . GPL17024-GPL17439-GPL18995-GPL25769 GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655). GPL18995: Illumina MiSeq (Escherichia coli BW25113). GPL25769: Illumina Genome Analyzer IIx (Escherichia coli BW25113) GSE122211 Expression profiling of E. coli K-12 MG1655 GSM3461156 MG_no_te_1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122211/GSE122211.soft.gz 100 50 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt glycerol stocks of E. coli strains were inoculated into 0.2%(w/v) glucose M9 minimal media. M9 minimal media was also supplemented with 1 ml trace element solution (100X), excluding MG_no_te_1 and MG_no_te_2. The culture was incubated at 37 ℃ overnight with agitation, and then was used to inoculate the fresh media. The fresh culture was incubated at 37 ℃ with agitation to the mid-log phase (OD600 ≈ 0.5). 10mM glutamate was added to the media MG_glu_1 and MG_glu_2. PGCGROWTHCONDITIONS SRR8164476 MCO000002697 37 ℃ Temperature True
growth_protocol 37.0 C glycerol stocks of E. coli strains were inoculated into 0.2 % ( w/v ) glucose M9 minimal media . <Med> M9 minimal media </Med> was also supplemented with 1 ml trace element solution ( 100X ) , excluding MG _ no _ te _ 1 and MG _ no _ te _ 2 . The culture was incubated at <Temp> 37 ℃ </Temp> overnight with agitation , and then was used to inoculate the fresh <Med> media </Med> . The fresh culture was incubated at 37 ℃ with agitation to the <Phase> mid-log phase </Phase> ( OD600 ≈ 0.5 ) . 10mM glutamate was added to the media MG _ glu _ 1 and MG _ glu _ 2 . GPL17024-GPL17439-GPL18995-GPL25769 GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655). GPL18995: Illumina MiSeq (Escherichia coli BW25113). GPL25769: Illumina Genome Analyzer IIx (Escherichia coli BW25113) GSE122211 Expression profiling of E. coli K-12 MG1655 GSM3461157 MG_no_te_2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122211/GSE122211.soft.gz 100 50 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt glycerol stocks of E. coli strains were inoculated into 0.2%(w/v) glucose M9 minimal media. M9 minimal media was also supplemented with 1 ml trace element solution (100X), excluding MG_no_te_1 and MG_no_te_2. The culture was incubated at 37 ℃ overnight with agitation, and then was used to inoculate the fresh media. The fresh culture was incubated at 37 ℃ with agitation to the mid-log phase (OD600 ≈ 0.5). 10mM glutamate was added to the media MG_glu_1 and MG_glu_2. PGCGROWTHCONDITIONS SRR8164477 MCO000002697 37 ℃ Temperature True
growth_protocol 37.0 C glycerol stocks of E. coli strains were inoculated into 0.2 % ( w/v ) glucose M9 minimal media . <Med> M9 minimal media </Med> was also supplemented with 1 ml trace element solution ( 100X ) , excluding MG _ no _ te _ 1 and MG _ no _ te _ 2 . The culture was incubated at <Temp> 37 ℃ </Temp> overnight with agitation , and then was used to inoculate the fresh <Med> media </Med> . The fresh culture was incubated at 37 ℃ with agitation to the <Phase> mid-log phase </Phase> ( OD600 ≈ 0.5 ) . 10mM glutamate was added to the media MG _ glu _ 1 and MG _ glu _ 2 . GPL17024-GPL17439-GPL18995-GPL25769 GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655). GPL18995: Illumina MiSeq (Escherichia coli BW25113). GPL25769: Illumina Genome Analyzer IIx (Escherichia coli BW25113) GSE122211 Expression profiling of E. coli K-12 MG1655 GSM3461164 MG_glu_1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122211/GSE122211.soft.gz 100 50 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt glycerol stocks of E. coli strains were inoculated into 0.2%(w/v) glucose M9 minimal media. M9 minimal media was also supplemented with 1 ml trace element solution (100X), excluding MG_no_te_1 and MG_no_te_2. The culture was incubated at 37 ℃ overnight with agitation, and then was used to inoculate the fresh media. The fresh culture was incubated at 37 ℃ with agitation to the mid-log phase (OD600 ≈ 0.5). 10mM glutamate was added to the media MG_glu_1 and MG_glu_2. PGCGROWTHCONDITIONS SRR8164484 MCO000002697 37 ℃ Temperature True
growth_protocol 37.0 C glycerol stocks of E. coli strains were inoculated into 0.2 % ( w/v ) glucose M9 minimal media . <Med> M9 minimal media </Med> was also supplemented with 1 ml trace element solution ( 100X ) , excluding MG _ no _ te _ 1 and MG _ no _ te _ 2 . The culture was incubated at <Temp> 37 ℃ </Temp> overnight with agitation , and then was used to inoculate the fresh <Med> media </Med> . The fresh culture was incubated at 37 ℃ with agitation to the <Phase> mid-log phase </Phase> ( OD600 ≈ 0.5 ) . 10mM glutamate was added to the media MG _ glu _ 1 and MG _ glu _ 2 . GPL17024-GPL17439-GPL18995-GPL25769 GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655). GPL18995: Illumina MiSeq (Escherichia coli BW25113). GPL25769: Illumina Genome Analyzer IIx (Escherichia coli BW25113) GSE122211 Expression profiling of E. coli K-12 MG1655 GSM3461165 MG_glu_2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122211/GSE122211.soft.gz 100 50 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt glycerol stocks of E. coli strains were inoculated into 0.2%(w/v) glucose M9 minimal media. M9 minimal media was also supplemented with 1 ml trace element solution (100X), excluding MG_no_te_1 and MG_no_te_2. The culture was incubated at 37 ℃ overnight with agitation, and then was used to inoculate the fresh media. The fresh culture was incubated at 37 ℃ with agitation to the mid-log phase (OD600 ≈ 0.5). 10mM glutamate was added to the media MG_glu_1 and MG_glu_2. PGCGROWTHCONDITIONS SRR8164485 MCO000002697 37 ℃ Temperature True
growth_protocol 37.0 C glycerol stocks of E. coli strains were inoculated into 0.2 % ( w/v ) glucose M9 minimal media . <Med> M9 minimal media </Med> was also supplemented with 1 ml trace element solution ( 100X ) , excluding MG _ no _ te _ 1 and MG _ no _ te _ 2 . The culture was incubated at <Temp> 37 ℃ </Temp> overnight with agitation , and then was used to inoculate the fresh <Med> media </Med> . The fresh culture was incubated at 37 ℃ with agitation to the <Phase> mid-log phase </Phase> ( OD600 ≈ 0.5 ) . 10mM glutamate was added to the media MG _ glu _ 1 and MG _ glu _ 2 . GPL17024-GPL17439-GPL18995-GPL25769 GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655). GPL18995: Illumina MiSeq (Escherichia coli BW25113). GPL25769: Illumina Genome Analyzer IIx (Escherichia coli BW25113) GSE122211 Expression profiling of E. coli K-12 MG1655 GSM3461166 MG_glc 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122211/GSE122211.soft.gz 100 50 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt glycerol stocks of E. coli strains were inoculated into 0.2%(w/v) glucose M9 minimal media. M9 minimal media was also supplemented with 1 ml trace element solution (100X), excluding MG_no_te_1 and MG_no_te_2. The culture was incubated at 37 ℃ overnight with agitation, and then was used to inoculate the fresh media. The fresh culture was incubated at 37 ℃ with agitation to the mid-log phase (OD600 ≈ 0.5). 10mM glutamate was added to the media MG_glu_1 and MG_glu_2. PGCGROWTHCONDITIONS SRR8164486 MCO000002697 37 ℃ Temperature True
characteristics glucose 2 g/L media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463565 wt_glc__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR8173227 MCO000003239 2g/L glucose Medium supplement True
characteristics glucose 2 g/L media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463566 wt_glc__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR8173228 MCO000003239 2g/L glucose Medium supplement True
characteristics glucose 2 g/L media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463567 wt_glc__3 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR8173229 MCO000003239 2g/L glucose Medium supplement True
characteristics glucose 2 g/L media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463568 wt_glc__4 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR8173230 MCO000003239 2g/L glucose Medium supplement True
characteristics glucose 2 g/L media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463573 gth__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR8173235 MCO000003239 2g/L glucose Medium supplement True
characteristics glucose 2 g/L media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463574 gth__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR8173236 MCO000003239 2g/L glucose Medium supplement True
characteristics glucose 2 g/L media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463577 met_glc__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR8173239 MCO000003239 2g/L glucose Medium supplement True
characteristics glucose 2 g/L media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463578 met_glc__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR8173240 MCO000003239 2g/L glucose Medium supplement True
characteristics glucose 2 g/L media : <Air> anaerobic </Air> <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463579 no3_anaero__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: anaerobic M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR8173241 MCO000003239 2g/L glucose Medium supplement True
characteristics glucose 2 g/L media : <Air> anaerobic </Air> <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463580 no3_anaero__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: anaerobic M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR8173242 MCO000003239 2g/L glucose Medium supplement True
characteristics glucose 2 g/L media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3854833 wt_glc_5__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR9204648 MCO000003239 2g/L glucose Medium supplement True
characteristics glucose 2 g/L media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3854834 wt_glc_6__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR9204649 MCO000003239 2g/L glucose Medium supplement True
characteristics glucose 2 g/L media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> and 100 mg/L adenine GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3854837 ade_glc__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose and 100 mg/L adenine PGCGROWTHCONDITIONS SRR9204652 MCO000003239 2g/L glucose Medium supplement True
characteristics glucose 2 g/L media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> and 100 mg/L adenine GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3854838 ade_glc__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose and 100 mg/L adenine PGCGROWTHCONDITIONS SRR9204653 MCO000003239 2g/L glucose Medium supplement True
characteristics glucose 2 g/L media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE122296 Expression profiling of multiple Escherichia coli strains on glucose minimal media GSM3463601 MG1655_1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122296/GSE122296.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR8173221 MCO000003239 2g/L glucose Medium supplement True
characteristics glucose 2 g/L media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE122296 Expression profiling of multiple Escherichia coli strains on glucose minimal media GSM3463602 MG1655_2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122296/GSE122296.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR8173222 MCO000003239 2g/L glucose Medium supplement True
characteristics mm supplement : <Anti> Cytidine </Anti> <Supp> ( 1 mM ) </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463571 cytd_rib__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 100 67 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json supplement: Cytidine (1 mM) PGCGROWTHCONDITIONS SRR8173233 MCO000011586 ( 1 mM ) Medium supplement True
characteristics mm supplement : <Anti> Cytidine </Anti> <Supp> ( 1 mM ) </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463572 cytd_rib__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 100 67 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json supplement: Cytidine (1 mM) PGCGROWTHCONDITIONS SRR8173234 MCO000011586 ( 1 mM ) Medium supplement True
characteristics glucose 120 mM media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose and 1 mM cytidine </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3854835 bw_delpurR_cytd__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 83 59 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose and 1 mM cytidine PGCGROWTHCONDITIONS SRR9204650 MCO000002775 2g/L glucose and 1 mM cytidine Medium supplement True
characteristics glucose 120 mM media : <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose and 1 mM cytidine </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3854836 bw_delpurR_cytd__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 83 59 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 2g/L glucose and 1 mM cytidine PGCGROWTHCONDITIONS SRR9204651 MCO000002775 2g/L glucose and 1 mM cytidine Medium supplement True
characteristics Organism <Gtype> material type </Gtype> : <Gtype> whole organism </Gtype> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE123554 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GSM3507068 delta-fis rep1 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE123554/GSE123554.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt material type: whole organism PGCGROWTHCONDITIONS SRR8309841 MCO000002467 whole organism Genetic background True
characteristics Organism <Gtype> material type </Gtype> : <Gtype> whole organism </Gtype> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE123554 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GSM3507069 delta-fis rep2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE123554/GSE123554.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt material type: whole organism PGCGROWTHCONDITIONS SRR8309842 MCO000002467 whole organism Genetic background True
characteristics Organism <Gtype> material type </Gtype> : <Gtype> whole organism </Gtype> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE123554 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GSM3507070 delta-hns rep1 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE123554/GSE123554.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt material type: whole organism PGCGROWTHCONDITIONS SRR8309843 MCO000002467 whole organism Genetic background True
characteristics Organism <Gtype> material type </Gtype> : <Gtype> whole organism </Gtype> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE123554 Reconstruction of transcriptional regulatory networks of Fis and H-NS in Escherichia coli from genome wide data analysis GSM3507071 delta-hns rep2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE123554/GSE123554.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt material type: whole organism PGCGROWTHCONDITIONS SRR8309844 MCO000002467 whole organism Genetic background True
characteristics wild type genotype/variation : <Gtype> naive ( wild type ) </Gtype> GPL26204 GPL26204: NextSeq 550 (Escherichia coli K-12) GSE126710 RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GSM3611666 E. coli K-12 MG1655_R1 [MG_1] 31208335 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE126710/GSE126710.soft.gz 100 75 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: naive (wild type) PGCGROWTHCONDITIONS SRR8587784 MCO000002470 naive ( wild type ) Genetic background True
characteristics wild type genotype/variation : <Gtype> naive ( wild type ) </Gtype> GPL26204 GPL26204: NextSeq 550 (Escherichia coli K-12) GSE126710 RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GSM3611667 E. coli K-12 MG1655_R2 [MG_2] 31208335 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE126710/GSE126710.soft.gz 100 75 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: naive (wild type) PGCGROWTHCONDITIONS SRR8587785 MCO000002470 naive ( wild type ) Genetic background True
characteristics wild type genotype/variation : <Gtype> naive ( wild type ) </Gtype> GPL26204 GPL26204: NextSeq 550 (Escherichia coli K-12) GSE126710 RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens GSM3611668 E. coli K-12 MG1655_R3 [MG_3] 31208335 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE126710/GSE126710.soft.gz 100 75 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: naive (wild type) PGCGROWTHCONDITIONS SRR8587786 MCO000002470 naive ( wild type ) Genetic background True
source_name wt <Gtype> WT </Gtype> _ <Phase> mid-log phase </Phase> bacteria culture GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013462 WT_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json WT_mid-log phase bacteria culture PGCGROWTHCONDITIONS SRR9919224 MCO000002470 WT Genetic background True
source_name wt <Gtype> WT </Gtype> _ <Phase> mid-log phase </Phase> bacteria culture GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013463 WT_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json WT_mid-log phase bacteria culture PGCGROWTHCONDITIONS SRR9919225 MCO000002470 WT Genetic background True
source_name wt <Gtype> WT </Gtype> _ H2O2 _ <Phase> mid-log phase </Phase> bacteria culture GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013464 WT_H2O2_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json WT_H2O2_mid-log phase bacteria culture PGCGROWTHCONDITIONS SRR9919226 MCO000002470 WT Genetic background True
source_name wt <Gtype> WT </Gtype> _ H2O2 _ <Phase> mid-log phase </Phase> bacteria culture GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013465 WT_H2O2_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json WT_H2O2_mid-log phase bacteria culture PGCGROWTHCONDITIONS SRR9919227 MCO000002470 WT Genetic background True
characteristics wt genotype : <Gtype> WT </Gtype> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275442 WT_LB_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: WT PGCGROWTHCONDITIONS SRR10907640 MCO000002470 WT Genetic background True
characteristics wt genotype : <Gtype> WT </Gtype> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275443 WT_LB_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: WT PGCGROWTHCONDITIONS SRR10907641 MCO000002470 WT Genetic background True
characteristics wt genotype : <Gtype> WT </Gtype> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275444 WT_EtOH_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: WT PGCGROWTHCONDITIONS SRR10907642 MCO000002470 WT Genetic background True
characteristics wt genotype : <Gtype> WT </Gtype> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275458 WT_01-KCl_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: WT PGCGROWTHCONDITIONS SRR10907656 MCO000002470 WT Genetic background True
characteristics wt genotype : <Gtype> WT </Gtype> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275459 WT_01-KCl_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: WT PGCGROWTHCONDITIONS SRR10907657 MCO000002470 WT Genetic background True
characteristics wt genotype : <Gtype> WT </Gtype> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275460 WT_115-KCl_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: WT PGCGROWTHCONDITIONS SRR10907658 MCO000002470 WT Genetic background True
characteristics wt genotype : <Gtype> WT </Gtype> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275461 WT_115-KCl_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: WT PGCGROWTHCONDITIONS SRR10907659 MCO000002470 WT Genetic background True
characteristics wt genotype : <Gtype> WT </Gtype> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275466 WT_M9-P_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: WT PGCGROWTHCONDITIONS SRR10907664 MCO000002470 WT Genetic background True
characteristics wt genotype : <Gtype> WT </Gtype> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275467 WT_M9-P_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: WT PGCGROWTHCONDITIONS SRR10907665 MCO000002470 WT Genetic background True
characteristics wt genotype : <Gtype> WT </Gtype> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275472 WT_ZnCl2_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: WT PGCGROWTHCONDITIONS SRR10907670 MCO000002470 WT Genetic background True
characteristics wt genotype : <Gtype> WT </Gtype> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275473 WT_ZnCl2_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: WT PGCGROWTHCONDITIONS SRR10907671 MCO000002470 WT Genetic background True
characteristics wt genotype : <Gtype> WT </Gtype> GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104402 WT_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: WT PGCGROWTHCONDITIONS SRR794848 MCO000002470 WT Genetic background True
characteristics wt genotype : <Gtype> WT </Gtype> GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104403 WT_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: WT PGCGROWTHCONDITIONS SRR794849 MCO000002470 WT Genetic background True
characteristics wt genotype : <Gtype> WT </Gtype> GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104404 WT_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: WT PGCGROWTHCONDITIONS SRR794850 MCO000002470 WT Genetic background True
characteristics wt genotype : <Gtype> WT </Gtype> GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104405 wt_T_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: WT PGCGROWTHCONDITIONS SRR794851 MCO000002470 WT Genetic background True
characteristics wt genotype : <Gtype> WT </Gtype> GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104406 wt_T_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: WT PGCGROWTHCONDITIONS SRR794852 MCO000002470 WT Genetic background True
characteristics wt genotype : <Gtype> WT </Gtype> GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104407 wt_T_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: WT PGCGROWTHCONDITIONS SRR794853 MCO000002470 WT Genetic background True
characteristics wt genotype : <Gtype> WT </Gtype> GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104408 wt_un_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: WT PGCGROWTHCONDITIONS SRR794854 MCO000002470 WT Genetic background True
characteristics wt genotype : <Gtype> WT </Gtype> GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104410 wt_un_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: WT PGCGROWTHCONDITIONS SRR794856 MCO000002470 WT Genetic background True
characteristics wt genotype : <Gtype> WT </Gtype> GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104423 WT_minus_2DG_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: WT PGCGROWTHCONDITIONS SRR794869 MCO000002470 WT Genetic background True
characteristics wt genotype : <Gtype> WT </Gtype> GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104424 WT_minus_2DG_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: WT PGCGROWTHCONDITIONS SRR794870 MCO000002470 WT Genetic background True
characteristics wt genotype : <Gtype> WT </Gtype> GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104425 WT_minus_2DG_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: WT PGCGROWTHCONDITIONS SRR794871 MCO000002470 WT Genetic background True
characteristics wt genotype : <Gtype> WT </Gtype> GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104426 WT_plus_2DG_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: WT PGCGROWTHCONDITIONS SRR794872 MCO000002470 WT Genetic background True
characteristics wt genotype : <Gtype> WT </Gtype> GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104427 WT_plus_2DG_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: WT PGCGROWTHCONDITIONS SRR794873 MCO000002470 WT Genetic background True
characteristics wt genotype : <Gtype> WT </Gtype> GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104428 WT_plus_2DG_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: WT PGCGROWTHCONDITIONS SRR794874 MCO000002470 WT Genetic background True
characteristics wt genotype/variation : <Gtype> WT </Gtype> GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326347 WT with Fe 1 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variation: WT PGCGROWTHCONDITIONS SRR1168133 MCO000002470 WT Genetic background True
characteristics wt genotype/variation : <Gtype> WT </Gtype> GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326348 WT with Fe 2 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variation: WT PGCGROWTHCONDITIONS SRR1168134 MCO000002470 WT Genetic background True
characteristics wt genotype/variation : <Gtype> WT </Gtype> GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326349 WT with DPD 1 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variation: WT PGCGROWTHCONDITIONS SRR1168135 MCO000002470 WT Genetic background True
characteristics wt genotype/variation : <Gtype> WT </Gtype> GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE54900 Deciphering the Fur transcriptional regulatory network highlights its complex role beyond iron metabolism in Escherichia coli [RNA-seq] GSM1326350 WT with DPD 2 (RNA-seq) 25222563 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54900/GSE54900.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variation: WT PGCGROWTHCONDITIONS SRR1168136 MCO000002470 WT Genetic background True
source_name wt <Gtype> WT </Gtype> _ <Supp> glucose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602347 WT glucose 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json WT_glucose PGCGROWTHCONDITIONS SRR1787590 MCO000002470 WT Genetic background True
characteristics wt genotype/variation : <Gtype> WT </Gtype> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602347 WT glucose 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variation: WT PGCGROWTHCONDITIONS SRR1787590 MCO000002470 WT Genetic background True
source_name wt <Gtype> WT </Gtype> _ <Supp> glucose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602348 WT glucose 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json WT_glucose PGCGROWTHCONDITIONS SRR1787591 MCO000002470 WT Genetic background True
characteristics wt genotype/variation : <Gtype> WT </Gtype> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602348 WT glucose 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variation: WT PGCGROWTHCONDITIONS SRR1787591 MCO000002470 WT Genetic background True
source_name wt <Gtype> WT </Gtype> _ <Supp> fructose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602349 WT fructose 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json WT_fructose PGCGROWTHCONDITIONS SRR1787592 MCO000002470 WT Genetic background True
characteristics wt genotype/variation : <Gtype> WT </Gtype> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602349 WT fructose 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variation: WT PGCGROWTHCONDITIONS SRR1787592 MCO000002470 WT Genetic background True
source_name wt <Gtype> WT </Gtype> _ <Supp> fructose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602350 WT fructose 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json WT_fructose PGCGROWTHCONDITIONS SRR1787593 MCO000002470 WT Genetic background True
characteristics wt genotype/variation : <Gtype> WT </Gtype> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602350 WT fructose 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variation: WT PGCGROWTHCONDITIONS SRR1787593 MCO000002470 WT Genetic background True
source_name wt <Gtype> WT </Gtype> _ <Supp> acetate </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602351 WT acetate 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json WT_acetate PGCGROWTHCONDITIONS SRR1787594 MCO000002470 WT Genetic background True
characteristics wt genotype/variation : <Gtype> WT </Gtype> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602351 WT acetate 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variation: WT PGCGROWTHCONDITIONS SRR1787594 MCO000002470 WT Genetic background True
source_name wt <Gtype> WT </Gtype> _ <Supp> acetate </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602352 WT acetate 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json WT_acetate PGCGROWTHCONDITIONS SRR1787595 MCO000002470 WT Genetic background True
characteristics wt genotype/variation : <Gtype> WT </Gtype> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602352 WT acetate 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variation: WT PGCGROWTHCONDITIONS SRR1787595 MCO000002470 WT Genetic background True
source_name wt <Gtype> WT </Gtype> <Supp> PQ </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603386 WT PQ 1 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json WT PQ PGCGROWTHCONDITIONS SRR1796598 MCO000002470 WT Genetic background True
characteristics wt genotype/variation : <Gtype> WT </Gtype> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603386 WT PQ 1 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variation: WT PGCGROWTHCONDITIONS SRR1796598 MCO000002470 WT Genetic background True
source_name wt <Gtype> WT </Gtype> <Supp> PQ </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603387 WT PQ 2 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json WT PQ PGCGROWTHCONDITIONS SRR1796599 MCO000002470 WT Genetic background True
characteristics wt genotype/variation : <Gtype> WT </Gtype> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603387 WT PQ 2 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variation: WT PGCGROWTHCONDITIONS SRR1796599 MCO000002470 WT Genetic background True
source_name wt <Gtype> WT </Gtype> <pH> pH5 .5 </pH> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623160 WT pH5.5 1 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json WT pH5.5 PGCGROWTHCONDITIONS SRR1824557 MCO000002470 WT Genetic background True
characteristics wt genotype/variation : <Gtype> WT </Gtype> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623160 WT pH5.5 1 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variation: WT PGCGROWTHCONDITIONS SRR1824557 MCO000002470 WT Genetic background True
source_name wt <Gtype> WT </Gtype> <pH> pH5 .5 </pH> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623161 WT pH5.5 2 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json WT pH5.5 PGCGROWTHCONDITIONS SRR1824558 MCO000002470 WT Genetic background True
characteristics wt genotype/variation : <Gtype> WT </Gtype> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623161 WT pH5.5 2 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variation: WT PGCGROWTHCONDITIONS SRR1824558 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900470 Parent LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547467 MCO000002470 WT Genetic background True
characteristics wt genotype/variation : <Gtype> WT </Gtype> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900470 Parent LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variation: WT PGCGROWTHCONDITIONS SRR2547467 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900472 cysG KO LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547469 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900474 cysH KO LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547471 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900475 cysH KO LB rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547472 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900476 dcd KO LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547473 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900477 dcd KO LB rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547474 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900478 fadr KO LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547475 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900479 fadr KO LB rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547476 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900480 ppk KO LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547477 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900482 wzc KO LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547479 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900483 wzc KO LB rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547480 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900485 yghD KO LB rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547482 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900486 fepA KO LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547483 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900488 lacA KO LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547485 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900490 Parent M9 rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547487 MCO000002470 WT Genetic background True
characteristics wt genotype/variation : <Gtype> WT </Gtype> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900490 Parent M9 rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variation: WT PGCGROWTHCONDITIONS SRR2547487 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900491 Parent M9 rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547488 MCO000002470 WT Genetic background True
characteristics wt genotype/variation : <Gtype> WT </Gtype> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900491 Parent M9 rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variation: WT PGCGROWTHCONDITIONS SRR2547488 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900492 dcd KO M9 rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547489 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900493 dcd KO M9 rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547490 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900494 fadr KO M9 rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547491 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900495 fadr KO M9 rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547492 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900496 ppk KO M9 rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547493 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900497 ppk KO M9 rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547494 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900498 wzc KO M9 rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547495 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900499 wzc KO M9 rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547496 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900500 yghD KO M9 rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547497 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900501 yghD KO M9 rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547498 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900502 fepA KO M9 rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547499 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900507 WT rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547504 MCO000002470 WT Genetic background True
characteristics wt genotype/variation : <Gtype> WT </Gtype> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900507 WT rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variation: WT PGCGROWTHCONDITIONS SRR2547504 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900509 mgtA KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547506 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900510 mgtA KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547507 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900513 gabT KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547510 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900514 gabT KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547511 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900515 sdhC KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547512 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900516 sdhC KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547513 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900518 putP KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547515 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900520 putP KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547517 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900521 rfbA KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547518 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900522 rfbA KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547519 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900524 entF KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547521 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900525 entF KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547522 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900526 entF KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547523 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900527 kefB KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547524 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900529 kefB KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547526 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900530 cysA KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547527 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900531 cysA KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547528 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900532 cysA KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547529 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900534 galE KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547531 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900536 mhpD KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547533 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900537 mhpD KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547534 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900538 mhpD KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547535 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900539 fliY KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547536 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900540 fliY KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547537 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900541 fliY KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547538 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900542 lplA KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547539 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900543 lplA KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547540 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900544 lplA KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547541 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900545 khc KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547542 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900546 khc KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547543 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900547 khc KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547544 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900548 ugpC KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547545 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900549 ugpC KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547546 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900550 ugpC KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547547 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900551 trpD KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547548 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900552 trpD KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547549 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900553 trpD KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547550 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900554 aspC KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547551 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900555 aspC KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547552 MCO000002470 WT Genetic background True
growth_protocol wt For the first batch ( from <Gtype> WT </Gtype> _ <Med> LB </Med> _ 1 to lacA _ M9 _ 2 ) , fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at <Temp> 37 °C </Temp> . 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3 % glucose and grown for 12 h at <Temp> 37 °C </Temp> . At 12 h , cells were harvested for the RNA-seq . For the second batch ( from <Gtype> WT </Gtype> _ 1 to aspC _ 3 ) , a 1:100 dilution from an overnight culture in M9 0.4 % glucose was done . When the late stationary phase was reached , the samples were harvested . GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900556 aspC KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json For the first batch (from WT_LB_1 to lacA_M9_2), fresh colonies of E. coli cells were taken and inoculated in 1 ml LB and grown for 8 h at 37°C. 20 µL of grown culture was taken and transferred to 3 ml LB/M9 medium supplemented with 0.3% glucose and grown for 12 h at 37°C. At 12 h, cells were harvested for the RNA-seq. For the second batch (from WT_1 to aspC_3), a 1:100 dilution from an overnight culture in M9 0.4% glucose was done. When the late stationary phase was reached, the samples were harvested. PGCGROWTHCONDITIONS SRR2547553 MCO000002470 WT Genetic background True
characteristics wt genotype/variaion : <Gtype> WT </Gtype> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968346 WTRep1_0min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variaion: WT PGCGROWTHCONDITIONS SRR2982421 MCO000002470 WT Genetic background True
characteristics wt genotype/variaion : <Gtype> WT </Gtype> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968347 WTRep1_2min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variaion: WT PGCGROWTHCONDITIONS SRR2982422 MCO000002470 WT Genetic background True
characteristics wt genotype/variaion : <Gtype> WT </Gtype> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968348 WTRep1_4min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variaion: WT PGCGROWTHCONDITIONS SRR2982423 MCO000002470 WT Genetic background True
characteristics wt genotype/variaion : <Gtype> WT </Gtype> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968349 WTRep1_6min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variaion: WT PGCGROWTHCONDITIONS SRR2982424 MCO000002470 WT Genetic background True
characteristics wt genotype/variaion : <Gtype> WT </Gtype> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968350 WTRep1_8min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variaion: WT PGCGROWTHCONDITIONS SRR2982425 MCO000002470 WT Genetic background True
characteristics wt genotype/variaion : <Gtype> WT </Gtype> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968351 WTRep1_10min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variaion: WT PGCGROWTHCONDITIONS SRR2982426 MCO000002470 WT Genetic background True
characteristics wt genotype/variaion : <Gtype> WT </Gtype> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968352 WTRep1_15min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variaion: WT PGCGROWTHCONDITIONS SRR2982427 MCO000002470 WT Genetic background True
characteristics wt genotype/variaion : <Gtype> WT </Gtype> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968353 WTRep1_20min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variaion: WT PGCGROWTHCONDITIONS SRR2982428 MCO000002470 WT Genetic background True
characteristics wt genotype/variaion : <Gtype> WT </Gtype> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968354 WTRep2_0min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variaion: WT PGCGROWTHCONDITIONS SRR2982429 MCO000002470 WT Genetic background True
characteristics wt genotype/variaion : <Gtype> WT </Gtype> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968355 WTRep2_2min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variaion: WT PGCGROWTHCONDITIONS SRR2982430 MCO000002470 WT Genetic background True
characteristics wt genotype/variaion : <Gtype> WT </Gtype> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968356 WTRep2_4min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variaion: WT PGCGROWTHCONDITIONS SRR2982431 MCO000002470 WT Genetic background True
characteristics wt genotype/variaion : <Gtype> WT </Gtype> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968357 WTRep2_6min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variaion: WT PGCGROWTHCONDITIONS SRR2982432 MCO000002470 WT Genetic background True
characteristics wt genotype/variaion : <Gtype> WT </Gtype> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968358 WTRep2_8min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variaion: WT PGCGROWTHCONDITIONS SRR2982433 MCO000002470 WT Genetic background True
characteristics wt genotype/variaion : <Gtype> WT </Gtype> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968359 WTRep2_10min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variaion: WT PGCGROWTHCONDITIONS SRR2982434 MCO000002470 WT Genetic background True
characteristics wt genotype/variaion : <Gtype> WT </Gtype> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968360 WTRep2_15min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variaion: WT PGCGROWTHCONDITIONS SRR2982435 MCO000002470 WT Genetic background True
characteristics wt genotype/variaion : <Gtype> WT </Gtype> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968361 WTRep2_20min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variaion: WT PGCGROWTHCONDITIONS SRR2982436 MCO000002470 WT Genetic background True
characteristics wt genotype : <Gtype> WT </Gtype> GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE88980 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GSM2356687 WT NaCl 1 28526842 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88980/GSE88980.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: WT PGCGROWTHCONDITIONS SRR4435464 MCO000002470 WT Genetic background True
characteristics wt genotype : <Gtype> WT </Gtype> GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE88980 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GSM2356688 WT NaCl 2 28526842 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88980/GSE88980.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: WT PGCGROWTHCONDITIONS SRR4435465 MCO000002470 WT Genetic background True
characteristics wt genotype/variation : <Gtype> -LCB- delta -RCB- perC : : kanR </Gtype> , coisogenic to <Gtype> WT </Gtype> GPL14548-GPL20262 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL20262: Illumina HiSeq 2000 (Escherichia coli O127:H6 str. E2348/69) GSE91001 PerC manipulates metabolism and surface antigens in enteropathogenic Escherichia coli GSM2418921 ATCACG-D1 28224117 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE91001/GSE91001.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variation: {delta}perC::kanR, coisogenic to WT PGCGROWTHCONDITIONS SRR5085370 MCO000002470 WT Genetic background True
characteristics wt genotype/variation : <Gtype> -LCB- delta -RCB- perC : : kanR </Gtype> , coisogenic to <Gtype> WT </Gtype> GPL14548-GPL20262 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL20262: Illumina HiSeq 2000 (Escherichia coli O127:H6 str. E2348/69) GSE91001 PerC manipulates metabolism and surface antigens in enteropathogenic Escherichia coli GSM2418922 CGATGT-D2 28224117 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE91001/GSE91001.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variation: {delta}perC::kanR, coisogenic to WT PGCGROWTHCONDITIONS SRR5085371 MCO000002470 WT Genetic background True
characteristics wt genotype/variation : <Gtype> -LCB- delta -RCB- perC : : kanR </Gtype> , coisogenic to <Gtype> WT </Gtype> GPL14548-GPL20262 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL20262: Illumina HiSeq 2000 (Escherichia coli O127:H6 str. E2348/69) GSE91001 PerC manipulates metabolism and surface antigens in enteropathogenic Escherichia coli GSM2418923 TTAGGC-D3 28224117 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE91001/GSE91001.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variation: {delta}perC::kanR, coisogenic to WT PGCGROWTHCONDITIONS SRR5085373 MCO000002470 WT Genetic background True
characteristics wt replicates : <Gtype> WT </Gtype> replicate 1 / induced GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433298 WT1_IPTG 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json replicates: WT replicate 1 / induced PGCGROWTHCONDITIONS SRR5121117 MCO000002470 WT Genetic background True
characteristics wt replicates : <Gtype> WT </Gtype> replicate 2 / induced GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433299 WT2_IPTG 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json replicates: WT replicate 2 / induced PGCGROWTHCONDITIONS SRR5121118 MCO000002470 WT Genetic background True
characteristics wt genotype/variation : <Gtype> WT </Gtype> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2564001 WT rep 1 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variation: WT PGCGROWTHCONDITIONS SRR5416993 MCO000002470 WT Genetic background True
characteristics wt genotype/variation : <Gtype> WT </Gtype> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2564002 WT rep 2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variation: WT PGCGROWTHCONDITIONS SRR5416994 MCO000002470 WT Genetic background True
characteristics wt genotype/variation : <Gtype> WT </Gtype> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2564003 WT rep 3 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype/variation: WT PGCGROWTHCONDITIONS SRR5416995 MCO000002470 WT Genetic background True
characteristics glucose 4 g/L media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013462 WT_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919224 MCO000002778 4g/L glucose Medium supplement True
characteristics glucose 4 g/L media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013463 WT_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919225 MCO000002778 4g/L glucose Medium supplement True
characteristics glucose 4 g/L media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013464 WT_H2O2_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919226 MCO000002778 4g/L glucose Medium supplement True
characteristics glucose 4 g/L media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013465 WT_H2O2_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919227 MCO000002778 4g/L glucose Medium supplement True
characteristics glucose 4 g/L media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013466 GMOS_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919228 MCO000002778 4g/L glucose Medium supplement True
characteristics glucose 4 g/L media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013467 GMOS_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919229 MCO000002778 4g/L glucose Medium supplement True
characteristics glucose 4 g/L media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013468 GMOS_H2O2_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919230 MCO000002778 4g/L glucose Medium supplement True
characteristics glucose 4 g/L media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013470 EC ALE-1_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919232 MCO000002778 4g/L glucose Medium supplement True
characteristics glucose 4 g/L media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013471 EC ALE-1_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919233 MCO000002778 4g/L glucose Medium supplement True
characteristics glucose 4 g/L media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013472 EC ALE-1_H2O2_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919234 MCO000002778 4g/L glucose Medium supplement True
characteristics glucose 4 g/L media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013473 EC ALE-1_H2O2_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919235 MCO000002778 4g/L glucose Medium supplement True
characteristics glucose 4 g/L media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013474 EC ALE-2_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919236 MCO000002778 4g/L glucose Medium supplement True
characteristics glucose 4 g/L media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013475 EC ALE-2_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919237 MCO000002778 4g/L glucose Medium supplement True
characteristics glucose 4 g/L media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013476 EC ALE-2_H2O2_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919238 MCO000002778 4g/L glucose Medium supplement True
characteristics glucose 4 g/L media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013477 EC ALE-2_H2O2_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919239 MCO000002778 4g/L glucose Medium supplement True
characteristics glucose 4 g/L media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013478 EC ALE-3_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919240 MCO000002778 4g/L glucose Medium supplement True
characteristics glucose 4 g/L media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013479 EC ALE-3_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919241 MCO000002778 4g/L glucose Medium supplement True
characteristics glucose 4 g/L media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013480 EC ALE-3_H2O2_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919242 MCO000002778 4g/L glucose Medium supplement True
characteristics glucose 4 g/L media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013481 EC ALE-3_H2O2_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919243 MCO000002778 4g/L glucose Medium supplement True
characteristics glucose 4 g/L media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013482 EC ALE-4_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919244 MCO000002778 4g/L glucose Medium supplement True
characteristics glucose 4 g/L media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013483 EC ALE-4_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919245 MCO000002778 4g/L glucose Medium supplement True
characteristics glucose 4 g/L media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013484 EC ALE-4_H2O2_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919246 MCO000002778 4g/L glucose Medium supplement True
characteristics glucose 4 g/L media : <Med> M9 minimal media </Med> w / <Supp> 4g/L glucose </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013485 EC ALE-4_H2O2_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 96 96 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal media w/ 4g/L glucose PGCGROWTHCONDITIONS SRR9919247 MCO000002778 4g/L glucose Medium supplement True
characteristics hydrogen peroxide 1 mM treatment : <Supp> 2 mM Hydrogen peroxide </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013464 WT_H2O2_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 95 95 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 2 mM Hydrogen peroxide PGCGROWTHCONDITIONS SRR9919226 MCO000003162 2 mM Hydrogen peroxide Medium supplement True
characteristics hydrogen peroxide 1 mM treatment : <Supp> 2 mM Hydrogen peroxide </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013465 WT_H2O2_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 95 95 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 2 mM Hydrogen peroxide PGCGROWTHCONDITIONS SRR9919227 MCO000003162 2 mM Hydrogen peroxide Medium supplement True
characteristics hydrogen peroxide 1 mM treatment : <Supp> 2 mM Hydrogen peroxide </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013468 GMOS_H2O2_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 95 95 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 2 mM Hydrogen peroxide PGCGROWTHCONDITIONS SRR9919230 MCO000003162 2 mM Hydrogen peroxide Medium supplement True
characteristics hydrogen peroxide 1 mM treatment : <Supp> 2 mM Hydrogen peroxide </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013472 EC ALE-1_H2O2_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 95 95 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 2 mM Hydrogen peroxide PGCGROWTHCONDITIONS SRR9919234 MCO000003162 2 mM Hydrogen peroxide Medium supplement True
characteristics hydrogen peroxide 1 mM treatment : <Supp> 2 mM Hydrogen peroxide </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013473 EC ALE-1_H2O2_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 95 95 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 2 mM Hydrogen peroxide PGCGROWTHCONDITIONS SRR9919235 MCO000003162 2 mM Hydrogen peroxide Medium supplement True
characteristics hydrogen peroxide 1 mM treatment : <Supp> 2 mM Hydrogen peroxide </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013476 EC ALE-2_H2O2_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 95 95 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 2 mM Hydrogen peroxide PGCGROWTHCONDITIONS SRR9919238 MCO000003162 2 mM Hydrogen peroxide Medium supplement True
characteristics hydrogen peroxide 1 mM treatment : <Supp> 2 mM Hydrogen peroxide </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013477 EC ALE-2_H2O2_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 95 95 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 2 mM Hydrogen peroxide PGCGROWTHCONDITIONS SRR9919239 MCO000003162 2 mM Hydrogen peroxide Medium supplement True
characteristics hydrogen peroxide 1 mM treatment : <Supp> 2 mM Hydrogen peroxide </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013480 EC ALE-3_H2O2_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 95 95 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 2 mM Hydrogen peroxide PGCGROWTHCONDITIONS SRR9919242 MCO000003162 2 mM Hydrogen peroxide Medium supplement True
characteristics hydrogen peroxide 1 mM treatment : <Supp> 2 mM Hydrogen peroxide </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013481 EC ALE-3_H2O2_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 95 95 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 2 mM Hydrogen peroxide PGCGROWTHCONDITIONS SRR9919243 MCO000003162 2 mM Hydrogen peroxide Medium supplement True
characteristics hydrogen peroxide 1 mM treatment : <Supp> 2 mM Hydrogen peroxide </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013484 EC ALE-4_H2O2_1 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 95 95 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 2 mM Hydrogen peroxide PGCGROWTHCONDITIONS SRR9919246 MCO000003162 2 mM Hydrogen peroxide Medium supplement True
characteristics hydrogen peroxide 1 mM treatment : <Supp> 2 mM Hydrogen peroxide </Supp> GPL24377 GPL24377: Illumina HiSeq 4000 (Escherichia coli K-12) GSE135516 OxyR is a convergent target for mutations acquired during adaptation to oxidative stress-prone metabolic states GSM4013485 EC ALE-4_H2O2_2 31651953 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE135516/GSE135516.soft.gz 95 95 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 2 mM Hydrogen peroxide PGCGROWTHCONDITIONS SRR9919247 MCO000003162 2 mM Hydrogen peroxide Medium supplement True
data_processing ion <Supp> Supplementary </Supp> _ files _ format _ and _ content : <Gtype> * </Gtype> _ strand.wig : <Gtype> In </Gtype> the wig files , the scores represent the counts of uniquely aligned reads whose 5 ' ends mapped . GPL23030-GPL28314 GPL23030: Illumina MiSeq (Escherichia coli str. K-12 substr. W3110). GPL28314: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. W3110) GSE147611 Nascent RNA sequencing identifies a widespread sigma70-dependent pausing regulated by Gre factors in bacteria GSM4435425 sigma70WT_RNA-seq_rep1 33568644 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE147611/GSE147611.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Supplementary_files_format_and_content: *_strand.wig: In the wig files, the scores represent the counts of uniquely aligned reads whose 5’ ends mapped. PGCGROWTHCONDITIONS SRR11431046 MCO000000580 In Genetic background True
data_processing ion <Supp> Supplementary </Supp> _ files _ format _ and _ content : <Gtype> * </Gtype> _ strand.wig : <Gtype> In </Gtype> the wig files , the scores represent the counts of uniquely aligned reads whose 5 ' ends mapped . GPL23030-GPL28314 GPL23030: Illumina MiSeq (Escherichia coli str. K-12 substr. W3110). GPL28314: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. W3110) GSE147611 Nascent RNA sequencing identifies a widespread sigma70-dependent pausing regulated by Gre factors in bacteria GSM4435426 sigma70WT_RNA-seq_rep2 33568644 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE147611/GSE147611.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Supplementary_files_format_and_content: *_strand.wig: In the wig files, the scores represent the counts of uniquely aligned reads whose 5’ ends mapped. PGCGROWTHCONDITIONS SRR11431047 MCO000000580 In Genetic background True
data_processing ion <Supp> Supplementary </Supp> _ files _ format _ and _ content : <Gtype> * </Gtype> _ strand.wig : <Gtype> In </Gtype> the wig files , the scores represent the counts of uniquely aligned reads whose 5 ' ends mapped . GPL23030-GPL28314 GPL23030: Illumina MiSeq (Escherichia coli str. K-12 substr. W3110). GPL28314: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. W3110) GSE147611 Nascent RNA sequencing identifies a widespread sigma70-dependent pausing regulated by Gre factors in bacteria GSM4435427 sigma70WT_RNA-seq_rep3 33568644 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE147611/GSE147611.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Supplementary_files_format_and_content: *_strand.wig: In the wig files, the scores represent the counts of uniquely aligned reads whose 5’ ends mapped. PGCGROWTHCONDITIONS SRR11431048 MCO000000580 In Genetic background True
data_processing ion <Supp> Supplementary </Supp> _ files _ format _ and _ content : <Gtype> * </Gtype> _ strand.wig : <Gtype> In </Gtype> the wig files , the scores represent the counts of uniquely aligned reads whose 5 ' ends mapped . GPL23030-GPL28314 GPL23030: Illumina MiSeq (Escherichia coli str. K-12 substr. W3110). GPL28314: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. W3110) GSE147611 Nascent RNA sequencing identifies a widespread sigma70-dependent pausing regulated by Gre factors in bacteria GSM4435428 sigma70greAB-_RNA-seq_rep1 33568644 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE147611/GSE147611.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Supplementary_files_format_and_content: *_strand.wig: In the wig files, the scores represent the counts of uniquely aligned reads whose 5’ ends mapped. PGCGROWTHCONDITIONS SRR11431049 MCO000000580 In Genetic background True
data_processing ion <Supp> Supplementary </Supp> _ files _ format _ and _ content : <Gtype> * </Gtype> _ strand.wig : <Gtype> In </Gtype> the wig files , the scores represent the counts of uniquely aligned reads whose 5 ' ends mapped . GPL23030-GPL28314 GPL23030: Illumina MiSeq (Escherichia coli str. K-12 substr. W3110). GPL28314: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. W3110) GSE147611 Nascent RNA sequencing identifies a widespread sigma70-dependent pausing regulated by Gre factors in bacteria GSM4435429 sigma70greAB-_RNA-seq_rep2 33568644 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE147611/GSE147611.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Supplementary_files_format_and_content: *_strand.wig: In the wig files, the scores represent the counts of uniquely aligned reads whose 5’ ends mapped. PGCGROWTHCONDITIONS SRR11431050 MCO000000580 In Genetic background True
data_processing ion <Supp> Supplementary </Supp> _ files _ format _ and _ content : <Gtype> * </Gtype> _ strand.wig : <Gtype> In </Gtype> the wig files , the scores represent the counts of uniquely aligned reads whose 5 ' ends mapped . GPL23030-GPL28314 GPL23030: Illumina MiSeq (Escherichia coli str. K-12 substr. W3110). GPL28314: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. W3110) GSE147611 Nascent RNA sequencing identifies a widespread sigma70-dependent pausing regulated by Gre factors in bacteria GSM4435430 sigma70greAB-_RNA-seq_rep3 33568644 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE147611/GSE147611.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Supplementary_files_format_and_content: *_strand.wig: In the wig files, the scores represent the counts of uniquely aligned reads whose 5’ ends mapped. PGCGROWTHCONDITIONS SRR11431051 MCO000000580 In Genetic background True
data_processing ion <Supp> Supplementary </Supp> _ files _ format _ and _ content : <Gtype> In </Gtype> the . txt files the first column is the coordinate of the base corresponding to NC _ 000913.3 and the second column is the number of normalized reads at that position . All 0 values were given a pseudocount of 0.01 . Counts are normalized so that the averages of ssrA , ssrS , and rnpB are stable throughout the decay from 0 to 20 minutes GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE95318 RNA-sequencing identifies new RNase III cleavage sites in E. coli and reveals increased regulation of mRNA GSM2507165 WTA_time0 28351917 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95318/GSE95318.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Supplementary_files_format_and_content: In the .txt files the first column is the coordinate of the base corresponding to NC_000913.3 and the second column is the number of normalized reads at that position. All 0 values were given a pseudocount of 0.01. Counts are normalized so that the averages of ssrA, ssrS, and rnpB are stable throughout the decay from 0 to 20 minutes PGCGROWTHCONDITIONS SRR5282299 MCO000000580 In Genetic background True
data_processing ion <Supp> Supplementary </Supp> _ files _ format _ and _ content : <Gtype> In </Gtype> the . txt files the first column is the coordinate of the base corresponding to NC _ 000913.3 and the second column is the number of normalized reads at that position . All 0 values were given a pseudocount of 0.01 . Counts are normalized so that the averages of ssrA , ssrS , and rnpB are stable throughout the decay from 0 to 20 minutes GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE95318 RNA-sequencing identifies new RNase III cleavage sites in E. coli and reveals increased regulation of mRNA GSM2507166 WTA_time2.5 28351917 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95318/GSE95318.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Supplementary_files_format_and_content: In the .txt files the first column is the coordinate of the base corresponding to NC_000913.3 and the second column is the number of normalized reads at that position. All 0 values were given a pseudocount of 0.01. Counts are normalized so that the averages of ssrA, ssrS, and rnpB are stable throughout the decay from 0 to 20 minutes PGCGROWTHCONDITIONS SRR5282300 MCO000000580 In Genetic background True
data_processing ion <Supp> Supplementary </Supp> _ files _ format _ and _ content : <Gtype> In </Gtype> the . txt files the first column is the coordinate of the base corresponding to NC _ 000913.3 and the second column is the number of normalized reads at that position . All 0 values were given a pseudocount of 0.01 . Counts are normalized so that the averages of ssrA , ssrS , and rnpB are stable throughout the decay from 0 to 20 minutes GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE95318 RNA-sequencing identifies new RNase III cleavage sites in E. coli and reveals increased regulation of mRNA GSM2507167 WTA_time5 28351917 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95318/GSE95318.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Supplementary_files_format_and_content: In the .txt files the first column is the coordinate of the base corresponding to NC_000913.3 and the second column is the number of normalized reads at that position. All 0 values were given a pseudocount of 0.01. Counts are normalized so that the averages of ssrA, ssrS, and rnpB are stable throughout the decay from 0 to 20 minutes PGCGROWTHCONDITIONS SRR5282301 MCO000000580 In Genetic background True
data_processing ion <Supp> Supplementary </Supp> _ files _ format _ and _ content : <Gtype> In </Gtype> the . txt files the first column is the coordinate of the base corresponding to NC _ 000913.3 and the second column is the number of normalized reads at that position . All 0 values were given a pseudocount of 0.01 . Counts are normalized so that the averages of ssrA , ssrS , and rnpB are stable throughout the decay from 0 to 20 minutes GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE95318 RNA-sequencing identifies new RNase III cleavage sites in E. coli and reveals increased regulation of mRNA GSM2507168 WTA_time7.5 28351917 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95318/GSE95318.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Supplementary_files_format_and_content: In the .txt files the first column is the coordinate of the base corresponding to NC_000913.3 and the second column is the number of normalized reads at that position. All 0 values were given a pseudocount of 0.01. Counts are normalized so that the averages of ssrA, ssrS, and rnpB are stable throughout the decay from 0 to 20 minutes PGCGROWTHCONDITIONS SRR5282302 MCO000000580 In Genetic background True
data_processing ion <Supp> Supplementary </Supp> _ files _ format _ and _ content : <Gtype> In </Gtype> the . txt files the first column is the coordinate of the base corresponding to NC _ 000913.3 and the second column is the number of normalized reads at that position . All 0 values were given a pseudocount of 0.01 . Counts are normalized so that the averages of ssrA , ssrS , and rnpB are stable throughout the decay from 0 to 20 minutes GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE95318 RNA-sequencing identifies new RNase III cleavage sites in E. coli and reveals increased regulation of mRNA GSM2507169 WTA_time10 28351917 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95318/GSE95318.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Supplementary_files_format_and_content: In the .txt files the first column is the coordinate of the base corresponding to NC_000913.3 and the second column is the number of normalized reads at that position. All 0 values were given a pseudocount of 0.01. Counts are normalized so that the averages of ssrA, ssrS, and rnpB are stable throughout the decay from 0 to 20 minutes PGCGROWTHCONDITIONS SRR5282303 MCO000000580 In Genetic background True
data_processing ion <Supp> Supplementary </Supp> _ files _ format _ and _ content : <Gtype> In </Gtype> the . txt files the first column is the coordinate of the base corresponding to NC _ 000913.3 and the second column is the number of normalized reads at that position . All 0 values were given a pseudocount of 0.01 . Counts are normalized so that the averages of ssrA , ssrS , and rnpB are stable throughout the decay from 0 to 20 minutes GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE95318 RNA-sequencing identifies new RNase III cleavage sites in E. coli and reveals increased regulation of mRNA GSM2507170 WTA_time20 28351917 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95318/GSE95318.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Supplementary_files_format_and_content: In the .txt files the first column is the coordinate of the base corresponding to NC_000913.3 and the second column is the number of normalized reads at that position. All 0 values were given a pseudocount of 0.01. Counts are normalized so that the averages of ssrA, ssrS, and rnpB are stable throughout the decay from 0 to 20 minutes PGCGROWTHCONDITIONS SRR5282304 MCO000000580 In Genetic background True
data_processing ion <Supp> Supplementary </Supp> _ files _ format _ and _ content : <Gtype> In </Gtype> the . txt files the first column is the coordinate of the base corresponding to NC _ 000913.3 and the second column is the number of normalized reads at that position . All 0 values were given a pseudocount of 0.01 . Counts are normalized so that the averages of ssrA , ssrS , and rnpB are stable throughout the decay from 0 to 20 minutes GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE95318 RNA-sequencing identifies new RNase III cleavage sites in E. coli and reveals increased regulation of mRNA GSM2507171 WTB_time0 28351917 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95318/GSE95318.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Supplementary_files_format_and_content: In the .txt files the first column is the coordinate of the base corresponding to NC_000913.3 and the second column is the number of normalized reads at that position. All 0 values were given a pseudocount of 0.01. Counts are normalized so that the averages of ssrA, ssrS, and rnpB are stable throughout the decay from 0 to 20 minutes PGCGROWTHCONDITIONS SRR5282305 MCO000000580 In Genetic background True
data_processing ion <Supp> Supplementary </Supp> _ files _ format _ and _ content : <Gtype> In </Gtype> the . txt files the first column is the coordinate of the base corresponding to NC _ 000913.3 and the second column is the number of normalized reads at that position . All 0 values were given a pseudocount of 0.01 . Counts are normalized so that the averages of ssrA , ssrS , and rnpB are stable throughout the decay from 0 to 20 minutes GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE95318 RNA-sequencing identifies new RNase III cleavage sites in E. coli and reveals increased regulation of mRNA GSM2507172 WTB_time2.5 28351917 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95318/GSE95318.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Supplementary_files_format_and_content: In the .txt files the first column is the coordinate of the base corresponding to NC_000913.3 and the second column is the number of normalized reads at that position. All 0 values were given a pseudocount of 0.01. Counts are normalized so that the averages of ssrA, ssrS, and rnpB are stable throughout the decay from 0 to 20 minutes PGCGROWTHCONDITIONS SRR5282306 MCO000000580 In Genetic background True
data_processing ion <Supp> Supplementary </Supp> _ files _ format _ and _ content : <Gtype> In </Gtype> the . txt files the first column is the coordinate of the base corresponding to NC _ 000913.3 and the second column is the number of normalized reads at that position . All 0 values were given a pseudocount of 0.01 . Counts are normalized so that the averages of ssrA , ssrS , and rnpB are stable throughout the decay from 0 to 20 minutes GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE95318 RNA-sequencing identifies new RNase III cleavage sites in E. coli and reveals increased regulation of mRNA GSM2507173 WTB_time7.5 28351917 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95318/GSE95318.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Supplementary_files_format_and_content: In the .txt files the first column is the coordinate of the base corresponding to NC_000913.3 and the second column is the number of normalized reads at that position. All 0 values were given a pseudocount of 0.01. Counts are normalized so that the averages of ssrA, ssrS, and rnpB are stable throughout the decay from 0 to 20 minutes PGCGROWTHCONDITIONS SRR5282307 MCO000000580 In Genetic background True
data_processing ion <Supp> Supplementary </Supp> _ files _ format _ and _ content : <Gtype> In </Gtype> the . txt files the first column is the coordinate of the base corresponding to NC _ 000913.3 and the second column is the number of normalized reads at that position . All 0 values were given a pseudocount of 0.01 . Counts are normalized so that the averages of ssrA , ssrS , and rnpB are stable throughout the decay from 0 to 20 minutes GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE95318 RNA-sequencing identifies new RNase III cleavage sites in E. coli and reveals increased regulation of mRNA GSM2507174 rnc-_time0 28351917 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95318/GSE95318.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Supplementary_files_format_and_content: In the .txt files the first column is the coordinate of the base corresponding to NC_000913.3 and the second column is the number of normalized reads at that position. All 0 values were given a pseudocount of 0.01. Counts are normalized so that the averages of ssrA, ssrS, and rnpB are stable throughout the decay from 0 to 20 minutes PGCGROWTHCONDITIONS SRR5282308 MCO000000580 In Genetic background True
data_processing ion <Supp> Supplementary </Supp> _ files _ format _ and _ content : <Gtype> In </Gtype> the . txt files the first column is the coordinate of the base corresponding to NC _ 000913.3 and the second column is the number of normalized reads at that position . All 0 values were given a pseudocount of 0.01 . Counts are normalized so that the averages of ssrA , ssrS , and rnpB are stable throughout the decay from 0 to 20 minutes GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE95318 RNA-sequencing identifies new RNase III cleavage sites in E. coli and reveals increased regulation of mRNA GSM2507175 rnc-_time2.5 28351917 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95318/GSE95318.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Supplementary_files_format_and_content: In the .txt files the first column is the coordinate of the base corresponding to NC_000913.3 and the second column is the number of normalized reads at that position. All 0 values were given a pseudocount of 0.01. Counts are normalized so that the averages of ssrA, ssrS, and rnpB are stable throughout the decay from 0 to 20 minutes PGCGROWTHCONDITIONS SRR5282309 MCO000000580 In Genetic background True
data_processing ion <Supp> Supplementary </Supp> _ files _ format _ and _ content : <Gtype> In </Gtype> the . txt files the first column is the coordinate of the base corresponding to NC _ 000913.3 and the second column is the number of normalized reads at that position . All 0 values were given a pseudocount of 0.01 . Counts are normalized so that the averages of ssrA , ssrS , and rnpB are stable throughout the decay from 0 to 20 minutes GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE95318 RNA-sequencing identifies new RNase III cleavage sites in E. coli and reveals increased regulation of mRNA GSM2507176 rnc-_time5 28351917 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95318/GSE95318.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Supplementary_files_format_and_content: In the .txt files the first column is the coordinate of the base corresponding to NC_000913.3 and the second column is the number of normalized reads at that position. All 0 values were given a pseudocount of 0.01. Counts are normalized so that the averages of ssrA, ssrS, and rnpB are stable throughout the decay from 0 to 20 minutes PGCGROWTHCONDITIONS SRR5282310 MCO000000580 In Genetic background True
data_processing ion <Supp> Supplementary </Supp> _ files _ format _ and _ content : <Gtype> In </Gtype> the . txt files the first column is the coordinate of the base corresponding to NC _ 000913.3 and the second column is the number of normalized reads at that position . All 0 values were given a pseudocount of 0.01 . Counts are normalized so that the averages of ssrA , ssrS , and rnpB are stable throughout the decay from 0 to 20 minutes GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE95318 RNA-sequencing identifies new RNase III cleavage sites in E. coli and reveals increased regulation of mRNA GSM2507177 rnc-_time7.5 28351917 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95318/GSE95318.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Supplementary_files_format_and_content: In the .txt files the first column is the coordinate of the base corresponding to NC_000913.3 and the second column is the number of normalized reads at that position. All 0 values were given a pseudocount of 0.01. Counts are normalized so that the averages of ssrA, ssrS, and rnpB are stable throughout the decay from 0 to 20 minutes PGCGROWTHCONDITIONS SRR5282311 MCO000000580 In Genetic background True
data_processing ion <Supp> Supplementary </Supp> _ files _ format _ and _ content : <Gtype> In </Gtype> the . txt files the first column is the coordinate of the base corresponding to NC _ 000913.3 and the second column is the number of normalized reads at that position . All 0 values were given a pseudocount of 0.01 . Counts are normalized so that the averages of ssrA , ssrS , and rnpB are stable throughout the decay from 0 to 20 minutes GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE95318 RNA-sequencing identifies new RNase III cleavage sites in E. coli and reveals increased regulation of mRNA GSM2507178 rnc-_time10 28351917 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95318/GSE95318.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Supplementary_files_format_and_content: In the .txt files the first column is the coordinate of the base corresponding to NC_000913.3 and the second column is the number of normalized reads at that position. All 0 values were given a pseudocount of 0.01. Counts are normalized so that the averages of ssrA, ssrS, and rnpB are stable throughout the decay from 0 to 20 minutes PGCGROWTHCONDITIONS SRR5282312 MCO000000580 In Genetic background True
data_processing ion <Supp> Supplementary </Supp> _ files _ format _ and _ content : <Gtype> In </Gtype> the . txt files the first column is the coordinate of the base corresponding to NC _ 000913.3 and the second column is the number of normalized reads at that position . All 0 values were given a pseudocount of 0.01 . Counts are normalized so that the averages of ssrA , ssrS , and rnpB are stable throughout the decay from 0 to 20 minutes GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE95318 RNA-sequencing identifies new RNase III cleavage sites in E. coli and reveals increased regulation of mRNA GSM2507179 rnc-_time20 28351917 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95318/GSE95318.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Supplementary_files_format_and_content: In the .txt files the first column is the coordinate of the base corresponding to NC_000913.3 and the second column is the number of normalized reads at that position. All 0 values were given a pseudocount of 0.01. Counts are normalized so that the averages of ssrA, ssrS, and rnpB are stable throughout the decay from 0 to 20 minutes PGCGROWTHCONDITIONS SRR5282313 MCO000000580 In Genetic background True
characteristics rifampicin 50 µM treatment group : <Supp> rifampicin time point 0 </Supp> GPL10328 GPL10328: Illumina Genome Analyzer II (Escherichia coli) GSE21341 genome-wide measurement of mRNA lifetime in Escherichia coli GSM533304 time point 0 20671182 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE21341/GSE21341.soft.gz 80 68 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment group: rifampicin time point 0 PGCGROWTHCONDITIONS SRR057747 MCO000011583 rifampicin time point 0 Medium supplement True
characteristics rifampicin treatment group : <Supp> rifampicin time point </Supp> 2 GPL10328 GPL10328: Illumina Genome Analyzer II (Escherichia coli) GSE21341 genome-wide measurement of mRNA lifetime in Escherichia coli GSM533305 time point 2 20671182 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE21341/GSE21341.soft.gz 100 65 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment group: rifampicin time point 2 PGCGROWTHCONDITIONS SRR057748 MCO000000828 rifampicin time point Medium supplement True
characteristics rifampicin 50 µM treatment group : <Supp> rifampicin time point 4 </Supp> GPL10328 GPL10328: Illumina Genome Analyzer II (Escherichia coli) GSE21341 genome-wide measurement of mRNA lifetime in Escherichia coli GSM533306 time point 4 20671182 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE21341/GSE21341.soft.gz 80 63 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment group: rifampicin time point 4 PGCGROWTHCONDITIONS SRR057749 MCO000011583 rifampicin time point 4 Medium supplement True
characteristics rifampicin 50 µM treatment group : <Supp> rifampicin time point 6 </Supp> GPL10328 GPL10328: Illumina Genome Analyzer II (Escherichia coli) GSE21341 genome-wide measurement of mRNA lifetime in Escherichia coli GSM533307 time point 6 20671182 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE21341/GSE21341.soft.gz 80 63 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment group: rifampicin time point 6 PGCGROWTHCONDITIONS SRR057750 MCO000011583 rifampicin time point 6 Medium supplement True
characteristics rifampicin 50 µM treatment group : <Supp> rifampicin time point 8 </Supp> GPL10328 GPL10328: Illumina Genome Analyzer II (Escherichia coli) GSE21341 genome-wide measurement of mRNA lifetime in Escherichia coli GSM533308 time point 8 20671182 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE21341/GSE21341.soft.gz 80 63 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment group: rifampicin time point 8 PGCGROWTHCONDITIONS SRR057751 MCO000011583 rifampicin time point 8 Medium supplement True
characteristics wild type genotype : <Gtype> wild-type </Gtype> GPL16760 GPL16760: Illumina Genome Analyzer II (Escherichia coli BL21(DE3)) GSE44928 Physiology study of Escherichia coli harboring high intracellular ATP driven by artificial Pck expression GSM1094082 BL21_2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE44928/GSE44928.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: wild-type PGCGROWTHCONDITIONS SRR771533 MCO000002470 wild-type Genetic background True
characteristics wild type genotype : <Gtype> wild-type </Gtype> GPL15206-GPL17096 GPL15206: Illumina HiSeq 2000 (Escherichia coli BW25113). GPL17096: Illumina HiSeq 2000 (Streptomyces coelicolor A3(2)) GSE46507 Comparison of the nucleotide-resolution, genome-wide, 5'-end maps of the transcriptomes of Escherichia coli K12 strain BW25113 and Streptomyces coelicolor A3(2) strain M145 GSM1131348 E. coli - TAP http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE46507/GSE46507.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: wild-type PGCGROWTHCONDITIONS SRR836184 MCO000002470 wild-type Genetic background True
characteristics wild type genotype : <Gtype> wild-type </Gtype> GPL15206-GPL17096 GPL15206: Illumina HiSeq 2000 (Escherichia coli BW25113). GPL17096: Illumina HiSeq 2000 (Streptomyces coelicolor A3(2)) GSE46507 Comparison of the nucleotide-resolution, genome-wide, 5'-end maps of the transcriptomes of Escherichia coli K12 strain BW25113 and Streptomyces coelicolor A3(2) strain M145 GSM1131349 E. coli + TAP http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE46507/GSE46507.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: wild-type PGCGROWTHCONDITIONS SRR836185 MCO000002470 wild-type Genetic background True
characteristics iptg condition : <Gtype> LB </Gtype> +3 g/L Glc +0.1 <Supp> mM IPTG </Supp> GPL16760 GPL16760: Illumina Genome Analyzer II (Escherichia coli BL21(DE3)) GSE44928 Physiology study of Escherichia coli harboring high intracellular ATP driven by artificial Pck expression GSM1094082 BL21_2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE44928/GSE44928.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json condition: LB+3g/L Glc +0.1mM IPTG PGCGROWTHCONDITIONS SRR771533 MCO000002105 mM IPTG Medium supplement True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104381 pHDB3_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794827 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104386 pLCV1_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794832 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104387 MG1655-aMG_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794833 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104388 MG1655-aMG_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794834 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104389 MG1655-aMG_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794835 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104390 MG1655+aMG_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794836 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104391 MG1655+aMG_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794837 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104392 MG1655+aMG_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794838 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104393 SgrR_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794839 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104394 SgrR_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794840 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104395 SgrR_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794841 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104396 sgrS_T_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794842 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104397 sgrS_T_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794843 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104398 sgrS_T_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794844 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104399 sgrS_un_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794845 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104400 sgrS_un_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794846 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104401 sgrS_un_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794847 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104402 WT_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794848 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104403 WT_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794849 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104404 WT_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794850 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104405 wt_T_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794851 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104406 wt_T_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794852 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104407 wt_T_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794853 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104408 wt_un_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794854 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104410 wt_un_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794856 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104411 CV108_minus_aMG_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794857 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104412 CV108_minus_aMG_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794858 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104413 CV108_minus_aMG_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794859 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104414 CV108_plus_aMG_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794860 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104415 CV108_plus_aMG_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794861 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104416 CV108_plus_aMG_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794862 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104417 MG1655_minus_aMG_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794863 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104418 MG1655_minus_aMG_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794864 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104419 MG1655_minus_aMG_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794865 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104420 MG1655_plus_aMG_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794866 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104421 MG1655_plus_aMG_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794867 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104422 MG1655_plus_aMG_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794868 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104423 WT_minus_2DG_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794869 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104424 WT_minus_2DG_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794870 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104425 WT_minus_2DG_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794871 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104426 WT_plus_2DG_1 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794872 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104427 WT_plus_2DG_2 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794873 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> growth at <Temp> 37 °C </Temp> in specified media GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE45443 RNA-seq analysis of Escherichia coli str. K-12 substr. MG1655 GSM1104428 WT_plus_2DG_3 23716638 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE45443/GSE45443.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic growth at 37°C in specified media PGCGROWTHCONDITIONS SRR794874 MCO000011679 Aerobic Aeration True
growth_protocol aerobic E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174823 Mid log_wildtype_glc minimal media_aerobic rep1 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922260 MCO000011679 Aerobic Aeration True
growth_protocol aerobic E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174824 Mid log_wildtype_glc minimal media_aerobic rep2 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922261 MCO000011679 Aerobic Aeration True
growth_protocol aerobic E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174825 Mid log_wildtype_glc minimal media_aerobic rep3 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922262 MCO000011679 Aerobic Aeration True
growth_protocol aerobic E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174826 Mid log_wildtype_glc minimal media + adenine_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922263 MCO000011679 Aerobic Aeration True
growth_protocol aerobic E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174827 Mid log_wildtype_glc minimal media + L-tryptophan_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922264 MCO000011679 Aerobic Aeration True
growth_protocol aerobic E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174828 Mid log_wildtype_glc minimal media_anaerobic rep1 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922265 MCO000011679 Aerobic Aeration True
growth_protocol aerobic E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174829 Mid log_wildtype_glc minimal media_anaerobic rep2 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922266 MCO000011679 Aerobic Aeration True
growth_protocol aerobic E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174830 Mid log_wildtype_glc minimal media_anaerobic rep3 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922267 MCO000011679 Aerobic Aeration True
growth_protocol aerobic E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174831 Mid log_nac KO_glc minimal media_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922268 MCO000011679 Aerobic Aeration True
growth_protocol aerobic E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174832 Mid log_nac KO_glc minimal media + adenine_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922269 MCO000011679 Aerobic Aeration True
growth_protocol aerobic E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174833 Mid log_cra KO_glc minimal media_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922270 MCO000011679 Aerobic Aeration True
growth_protocol aerobic E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174834 Mid log_cra KO_glc minimal media + L-tryptophan_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922271 MCO000011679 Aerobic Aeration True
growth_protocol aerobic E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174835 Mid log_mntR KO_glc minimal media_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922272 MCO000011679 Aerobic Aeration True
growth_protocol aerobic E. coli cultures were grown at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> with glucose as the primary carbon source and harvested at mid exponetial phase . <Air> Aerobic </Air> E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles . Condition specific media supplementation was added as described else where . GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174836 Mid log_mntR KO_glc minimal media_anaerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli cultures were grown at 37 °C in M9 minimal media with glucose as the primary carbon source and harvested at mid exponetial phase. Aerobic E. coli conditions were grown in shake flasks and anaerobic conditions were grown in anoxic serum bottles. Condition specific media supplementation was added as described else where. PGCGROWTHCONDITIONS SRR922273 MCO000011679 Aerobic Aeration True
source_name aerobic <Air> Aerobic </Air> culture GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360031 Wild-type (MG1655) T0 RNA rep 2 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic culture PGCGROWTHCONDITIONS SRR1211036 MCO000011679 Aerobic Aeration True
source_name aerobic <Air> Aerobic </Air> culture GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360032 Wild-type (MG1655) T1 RNA rep 1 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic culture PGCGROWTHCONDITIONS SRR1211037 MCO000011679 Aerobic Aeration True
source_name aerobic <Air> Aerobic </Air> culture GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360033 Wild-type (MG1655) T1 RNA rep 2 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic culture PGCGROWTHCONDITIONS SRR1211038 MCO000011679 Aerobic Aeration True
source_name aerobic <Air> Aerobic </Air> culture GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360034 Wild-type (MG1655) T2 RNA rep 1 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic culture PGCGROWTHCONDITIONS SRR1211039 MCO000011679 Aerobic Aeration True
source_name aerobic <Air> Aerobic </Air> culture GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360035 Wild-type (MG1655) T2 RNA rep 2 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic culture PGCGROWTHCONDITIONS SRR1211040 MCO000011679 Aerobic Aeration True
source_name aerobic <Air> Aerobic </Air> culture GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360036 Mutant (EP61) T0 RNA rep 1 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic culture PGCGROWTHCONDITIONS SRR1211041 MCO000011679 Aerobic Aeration True
source_name aerobic <Air> Aerobic </Air> culture GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360037 Mutant (EP61) T0 RNA rep 2 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic culture PGCGROWTHCONDITIONS SRR1211042 MCO000011679 Aerobic Aeration True
source_name aerobic <Air> Aerobic </Air> culture GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360038 Mutant (EP61) T1 RNA rep 1 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic culture PGCGROWTHCONDITIONS SRR1211043 MCO000011679 Aerobic Aeration True
source_name aerobic <Air> Aerobic </Air> culture GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360039 Mutant (EP61) T1 RNA rep 2 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic culture PGCGROWTHCONDITIONS SRR1211044 MCO000011679 Aerobic Aeration True
source_name aerobic <Air> Aerobic </Air> culture GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360040 Mutant (EP61) T2 RNA rep 1 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic culture PGCGROWTHCONDITIONS SRR1211045 MCO000011679 Aerobic Aeration True
source_name aerobic <Air> Aerobic </Air> culture GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360041 Mutant (EP61) T2 RNA rep 2 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic culture PGCGROWTHCONDITIONS SRR1211046 MCO000011679 Aerobic Aeration True
source_name aerobic <Air> Aerobic </Air> culture GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360042 Wild-type (MG1655) T0 RP rep 1 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic culture PGCGROWTHCONDITIONS SRR1211047 MCO000011679 Aerobic Aeration True
source_name aerobic <Air> Aerobic </Air> culture GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360043 Wild-type (MG1655) T0 RP rep 2 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic culture PGCGROWTHCONDITIONS SRR1211048 MCO000011679 Aerobic Aeration True
source_name aerobic <Air> Aerobic </Air> culture GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360044 Wild-type (MG1655) T1 RP rep 1 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic culture PGCGROWTHCONDITIONS SRR1211049 MCO000011679 Aerobic Aeration True
source_name aerobic <Air> Aerobic </Air> culture GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360045 Wild-type (MG1655) T1 RP rep 2 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic culture PGCGROWTHCONDITIONS SRR1211050 MCO000011679 Aerobic Aeration True
source_name aerobic <Air> Aerobic </Air> culture GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360046 Wild-type (MG1655) T2 RP rep 1 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic culture PGCGROWTHCONDITIONS SRR1211051 MCO000011679 Aerobic Aeration True
source_name aerobic <Air> Aerobic </Air> culture GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360047 Wild-type (MG1655) T2 RP rep 2 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic culture PGCGROWTHCONDITIONS SRR1211052 MCO000011679 Aerobic Aeration True
source_name aerobic <Air> Aerobic </Air> culture GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360048 Mutant (EP61) T0 RP rep 1 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic culture PGCGROWTHCONDITIONS SRR1211053 MCO000011679 Aerobic Aeration True
source_name aerobic <Air> Aerobic </Air> culture GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360049 Mutant (EP61) T0 RP rep 2 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic culture PGCGROWTHCONDITIONS SRR1211054 MCO000011679 Aerobic Aeration True
source_name aerobic <Air> Aerobic </Air> culture GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360050 Mutant (EP61) T1 RP rep 1 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic culture PGCGROWTHCONDITIONS SRR1211055 MCO000011679 Aerobic Aeration True
source_name aerobic <Air> Aerobic </Air> culture GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360051 Mutant (EP61) T1 RP rep 2 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic culture PGCGROWTHCONDITIONS SRR1211056 MCO000011679 Aerobic Aeration True
source_name aerobic <Air> Aerobic </Air> culture GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360052 Mutant (EP61) T2 RP rep 1 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic culture PGCGROWTHCONDITIONS SRR1211057 MCO000011679 Aerobic Aeration True
source_name aerobic <Air> Aerobic </Air> culture GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE56372 Correcting Direct Effects of Ethanol on Translation and Transcription Machinery Confers Ethanol Tolerance in Bacteria [Ribosome_Profiling] GSM1360053 Mutant (EP61) T2 RP rep 2 24927582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE56372/GSE56372.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic culture PGCGROWTHCONDITIONS SRR1211058 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> and anaerobic cultures were grown in 600 mL aliquots of DM25 and incubated at <Temp> 37 °C </Temp> with an orbital shaking of 150 RPM and inoculated with 1/100th volume of overnight culture . Aerobic cultures were grown for 9 h while anaerobic cultures were grown for 16 h. GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE80451 Transcriptome analysis of E. coli REL4536 grown aerobically and anaerobically GSM2127617 Aerobic 1 28103245 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80451/GSE80451.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic and anaerobic cultures were grown in 600 mL aliquots of DM25 and incubated at 37°C with an orbital shaking of 150 RPM and inoculated with 1/100th volume of overnight culture. Aerobic cultures were grown for 9 h while anaerobic cultures were grown for 16 h. PGCGROWTHCONDITIONS SRR3403686 MCO000011679 Aerobic Aeration True
characteristics aerobic growth environment : <Air> Aerobic </Air> GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE80451 Transcriptome analysis of E. coli REL4536 grown aerobically and anaerobically GSM2127617 Aerobic 1 28103245 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80451/GSE80451.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth environment: Aerobic PGCGROWTHCONDITIONS SRR3403686 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> and anaerobic cultures were grown in 600 mL aliquots of DM25 and incubated at <Temp> 37 °C </Temp> with an orbital shaking of 150 RPM and inoculated with 1/100th volume of overnight culture . Aerobic cultures were grown for 9 h while anaerobic cultures were grown for 16 h. GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE80451 Transcriptome analysis of E. coli REL4536 grown aerobically and anaerobically GSM2127618 Aerobic 2 28103245 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80451/GSE80451.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic and anaerobic cultures were grown in 600 mL aliquots of DM25 and incubated at 37°C with an orbital shaking of 150 RPM and inoculated with 1/100th volume of overnight culture. Aerobic cultures were grown for 9 h while anaerobic cultures were grown for 16 h. PGCGROWTHCONDITIONS SRR3403687 MCO000011679 Aerobic Aeration True
characteristics aerobic growth environment : <Air> Aerobic </Air> GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE80451 Transcriptome analysis of E. coli REL4536 grown aerobically and anaerobically GSM2127618 Aerobic 2 28103245 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80451/GSE80451.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth environment: Aerobic PGCGROWTHCONDITIONS SRR3403687 MCO000011679 Aerobic Aeration True
growth_protocol aerobic <Air> Aerobic </Air> and anaerobic cultures were grown in 600 mL aliquots of DM25 and incubated at <Temp> 37 °C </Temp> with an orbital shaking of 150 RPM and inoculated with 1/100th volume of overnight culture . Aerobic cultures were grown for 9 h while anaerobic cultures were grown for 16 h. GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE80451 Transcriptome analysis of E. coli REL4536 grown aerobically and anaerobically GSM2127619 Aerobic 3 28103245 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80451/GSE80451.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic and anaerobic cultures were grown in 600 mL aliquots of DM25 and incubated at 37°C with an orbital shaking of 150 RPM and inoculated with 1/100th volume of overnight culture. Aerobic cultures were grown for 9 h while anaerobic cultures were grown for 16 h. PGCGROWTHCONDITIONS SRR3403688 MCO000011679 Aerobic Aeration True
characteristics aerobic growth environment : <Air> Aerobic </Air> GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE80451 Transcriptome analysis of E. coli REL4536 grown aerobically and anaerobically GSM2127619 Aerobic 3 28103245 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80451/GSE80451.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth environment: Aerobic PGCGROWTHCONDITIONS SRR3403688 MCO000011679 Aerobic Aeration True
growth_protocol W2 minimal medium E. coli K12 MG1655 was grown to <Phase> mid-log phase </Phase> ( <OD> O.D. 600nm 0.5 </OD> ) or to stationary phase ( <OD> O.D. 600nm 1.5 </OD> ) <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> or <Med> W2 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> and 0.2 % glutamine . For heatshock conditions , cells were grown to mid-log phase and incubated at 42oC for 10 min . GPL17137 GPL17137: Illumina Genome Analyzer (Escherichia coli str. K-12 substr. MG1655) GSE46737 Genome-scale reconstruction of the sigma factor network in E. coli GSM1137316 E. coli stationary 1 24461193 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE46737/GSE46737.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 MG1655 was grown to mid-log phase (O.D.600nm 0.5) or to stationary phase (O.D.600nm 1.5) aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose or W2 minimal media supplemented with 0.2% glucose and 0.2% glutamine. For heatshock conditions, cells were grown to mid-log phase and incubated at 42oC for 10 min. PGCGROWTHCONDITIONS SRR847728 MCO000003317 W2 minimal media Medium True
growth_protocol W2 minimal medium E. coli K12 MG1655 was grown to <Phase> mid-log phase </Phase> ( <OD> O.D. 600nm 0.5 </OD> ) or to stationary phase ( <OD> O.D. 600nm 1.5 </OD> ) <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> or <Med> W2 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> and 0.2 % glutamine . For heatshock conditions , cells were grown to mid-log phase and incubated at 42oC for 10 min . GPL17137 GPL17137: Illumina Genome Analyzer (Escherichia coli str. K-12 substr. MG1655) GSE46737 Genome-scale reconstruction of the sigma factor network in E. coli GSM1137318 E. coli glutamine 1 24461193 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE46737/GSE46737.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 MG1655 was grown to mid-log phase (O.D.600nm 0.5) or to stationary phase (O.D.600nm 1.5) aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose or W2 minimal media supplemented with 0.2% glucose and 0.2% glutamine. For heatshock conditions, cells were grown to mid-log phase and incubated at 42oC for 10 min. PGCGROWTHCONDITIONS SRR847730 MCO000003317 W2 minimal media Medium True
growth_protocol W2 minimal medium E. coli K12 MG1655 was grown to <Phase> mid-log phase </Phase> ( <OD> O.D. 600nm 0.5 </OD> ) or to stationary phase ( <OD> O.D. 600nm 1.5 </OD> ) <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> or <Med> W2 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> and 0.2 % glutamine . For heatshock conditions , cells were grown to mid-log phase and incubated at 42oC for 10 min . GPL17137 GPL17137: Illumina Genome Analyzer (Escherichia coli str. K-12 substr. MG1655) GSE46737 Genome-scale reconstruction of the sigma factor network in E. coli GSM1137319 E. coli glutamine 2 24461193 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE46737/GSE46737.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 MG1655 was grown to mid-log phase (O.D.600nm 0.5) or to stationary phase (O.D.600nm 1.5) aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose or W2 minimal media supplemented with 0.2% glucose and 0.2% glutamine. For heatshock conditions, cells were grown to mid-log phase and incubated at 42oC for 10 min. PGCGROWTHCONDITIONS SRR847731 MCO000003317 W2 minimal media Medium True
growth_protocol W2 minimal medium E. coli K12 MG1655 was grown to <Phase> mid-log phase </Phase> ( <OD> O.D. 600nm 0.5 </OD> ) or to stationary phase ( <OD> O.D. 600nm 1.5 </OD> ) <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> or <Med> W2 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> and 0.2 % glutamine . For heatshock conditions , cells were grown to mid-log phase and incubated at 42oC for 10 min . GPL17137 GPL17137: Illumina Genome Analyzer (Escherichia coli str. K-12 substr. MG1655) GSE46737 Genome-scale reconstruction of the sigma factor network in E. coli GSM1137320 E. coli heatshock 1 24461193 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE46737/GSE46737.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 MG1655 was grown to mid-log phase (O.D.600nm 0.5) or to stationary phase (O.D.600nm 1.5) aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose or W2 minimal media supplemented with 0.2% glucose and 0.2% glutamine. For heatshock conditions, cells were grown to mid-log phase and incubated at 42oC for 10 min. PGCGROWTHCONDITIONS SRR847732 MCO000003317 W2 minimal media Medium True
growth_protocol W2 minimal medium E. coli K12 MG1655 was grown to <Phase> mid-log phase </Phase> ( <OD> O.D. 600nm 0.5 </OD> ) or to stationary phase ( <OD> O.D. 600nm 1.5 </OD> ) <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> or <Med> W2 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> and 0.2 % glutamine . For heatshock conditions , cells were grown to mid-log phase and incubated at 42oC for 10 min . GPL17137 GPL17137: Illumina Genome Analyzer (Escherichia coli str. K-12 substr. MG1655) GSE46737 Genome-scale reconstruction of the sigma factor network in E. coli GSM1137321 E. coli heatshock 2 24461193 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE46737/GSE46737.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K12 MG1655 was grown to mid-log phase (O.D.600nm 0.5) or to stationary phase (O.D.600nm 1.5) aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose or W2 minimal media supplemented with 0.2% glucose and 0.2% glutamine. For heatshock conditions, cells were grown to mid-log phase and incubated at 42oC for 10 min. PGCGROWTHCONDITIONS SRR847733 MCO000003317 W2 minimal media Medium True
source_name L-glutamine 0.2% Cells at mid-log phase ( OD600nm 0.5 ) in W2 media supplemented with <Supp> 0.2 % glucose </Supp> and <Supp> 0.2 % glutamine </Supp> GPL17137 GPL17137: Illumina Genome Analyzer (Escherichia coli str. K-12 substr. MG1655) GSE46737 Genome-scale reconstruction of the sigma factor network in E. coli GSM1137318 E. coli glutamine 1 24461193 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE46737/GSE46737.soft.gz 100 93 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells at mid-log phase (OD600nm 0.5) in W2 media supplemented with 0.2% glucose and 0.2% glutamine PGCGROWTHCONDITIONS SRR847730 MCO000002720 0.2 % glutamine Medium supplement True
source_name L-glutamine 0.2% Cells at mid-log phase ( OD600nm 0.5 ) in W2 media supplemented with <Supp> 0.2 % glucose </Supp> and <Supp> 0.2 % glutamine </Supp> GPL17137 GPL17137: Illumina Genome Analyzer (Escherichia coli str. K-12 substr. MG1655) GSE46737 Genome-scale reconstruction of the sigma factor network in E. coli GSM1137319 E. coli glutamine 2 24461193 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE46737/GSE46737.soft.gz 100 93 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells at mid-log phase (OD600nm 0.5) in W2 media supplemented with 0.2% glucose and 0.2% glutamine PGCGROWTHCONDITIONS SRR847731 MCO000002720 0.2 % glutamine Medium supplement True
characteristics MOPS culture/growth condition : <Supp> MOPS-P 2h </Supp> GPL10328-GPL14548 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE48151 Directional RNA-seq reveals highly complex condition-dependent transcriptomes in E. coliK12 through accurate full-length transcripts assembling GSM1170035 M-P2h_r1_HiSeq 23899370 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48151/GSE48151.soft.gz 100 62 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt culture/growth condition: MOPS-P 2h PGCGROWTHCONDITIONS SRR915696 MCO000001665 MOPS-P 2h Medium supplement True
characteristics MOPS culture/growth condition : <Supp> MOPS-P 2h </Supp> GPL10328-GPL14548 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE48151 Directional RNA-seq reveals highly complex condition-dependent transcriptomes in E. coliK12 through accurate full-length transcripts assembling GSM1170036 M-P2h_r2_HiSeq 23899370 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48151/GSE48151.soft.gz 100 62 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt culture/growth condition: MOPS-P 2h PGCGROWTHCONDITIONS SRR915697 MCO000001665 MOPS-P 2h Medium supplement True
source_name wt <Gtype> WT WT </Gtype> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174823 Mid log_wildtype_glc minimal media_aerobic rep1 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 57 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json WT PGCGROWTHCONDITIONS SRR922260 MCO000002470 WT WT Genetic background True
source_name wt <Gtype> WT WT </Gtype> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174824 Mid log_wildtype_glc minimal media_aerobic rep2 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 57 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json WT PGCGROWTHCONDITIONS SRR922261 MCO000002470 WT WT Genetic background True
source_name wt <Gtype> WT WT </Gtype> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174825 Mid log_wildtype_glc minimal media_aerobic rep3 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 57 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json WT PGCGROWTHCONDITIONS SRR922262 MCO000002470 WT WT Genetic background True
source_name wt <Gtype> WT WT </Gtype> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2564001 WT rep 1 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 100 57 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json WT PGCGROWTHCONDITIONS SRR5416993 MCO000002470 WT WT Genetic background True
source_name wt <Gtype> WT WT </Gtype> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2564002 WT rep 2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 100 57 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json WT PGCGROWTHCONDITIONS SRR5416994 MCO000002470 WT WT Genetic background True
source_name wt <Gtype> WT WT </Gtype> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2564003 WT rep 3 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 100 57 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json WT PGCGROWTHCONDITIONS SRR5416995 MCO000002470 WT WT Genetic background True
characteristics wild type genotype/variation : <Gtype> wild type ; MG1655 </Gtype> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174823 Mid log_wildtype_glc minimal media_aerobic rep1 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 72 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type; MG1655 PGCGROWTHCONDITIONS SRR922260 MCO000002470 wild type ; MG1655 Genetic background True
characteristics wild type genotype/variation : <Gtype> wild type ; MG1655 </Gtype> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174824 Mid log_wildtype_glc minimal media_aerobic rep2 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 72 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type; MG1655 PGCGROWTHCONDITIONS SRR922261 MCO000002470 wild type ; MG1655 Genetic background True
characteristics wild type genotype/variation : <Gtype> wild type ; MG1655 </Gtype> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174825 Mid log_wildtype_glc minimal media_aerobic rep3 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 72 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type; MG1655 PGCGROWTHCONDITIONS SRR922262 MCO000002470 wild type ; MG1655 Genetic background True
characteristics wild type genotype/variation : <Gtype> wild type ; MG1655 </Gtype> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174826 Mid log_wildtype_glc minimal media + adenine_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 72 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type; MG1655 PGCGROWTHCONDITIONS SRR922263 MCO000002470 wild type ; MG1655 Genetic background True
characteristics wild type genotype/variation : <Gtype> wild type ; MG1655 </Gtype> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174827 Mid log_wildtype_glc minimal media + L-tryptophan_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 72 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type; MG1655 PGCGROWTHCONDITIONS SRR922264 MCO000002470 wild type ; MG1655 Genetic background True
characteristics wild type genotype/variation : <Gtype> wild type ; MG1655 </Gtype> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174828 Mid log_wildtype_glc minimal media_anaerobic rep1 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 72 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type; MG1655 PGCGROWTHCONDITIONS SRR922265 MCO000002470 wild type ; MG1655 Genetic background True
characteristics wild type genotype/variation : <Gtype> wild type ; MG1655 </Gtype> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174829 Mid log_wildtype_glc minimal media_anaerobic rep2 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 72 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type; MG1655 PGCGROWTHCONDITIONS SRR922266 MCO000002470 wild type ; MG1655 Genetic background True
characteristics wild type genotype/variation : <Gtype> wild type ; MG1655 </Gtype> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174830 Mid log_wildtype_glc minimal media_anaerobic rep3 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 72 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type; MG1655 PGCGROWTHCONDITIONS SRR922267 MCO000002470 wild type ; MG1655 Genetic background True
characteristics glucose 2 g/l basal media : <Med> M9 + 4 g/L glc ( glucose minimal media ) </Med> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174823 Mid log_wildtype_glc minimal media_aerobic rep1 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 92 51 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json basal media: M9 + 4 g/L glc (glucose minimal media) PGCGROWTHCONDITIONS SRR922260 MCO000002776 M9 + 4 g/L glc ( glucose minimal media ) Medium True
characteristics glucose 2 g/l basal media : <Med> M9 + 4 g/L glc ( glucose minimal media ) </Med> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174824 Mid log_wildtype_glc minimal media_aerobic rep2 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 92 51 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json basal media: M9 + 4 g/L glc (glucose minimal media) PGCGROWTHCONDITIONS SRR922261 MCO000002776 M9 + 4 g/L glc ( glucose minimal media ) Medium True
characteristics glucose 2 g/l basal media : <Med> M9 + 4 g/L glc ( glucose minimal media ) </Med> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174825 Mid log_wildtype_glc minimal media_aerobic rep3 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 92 51 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json basal media: M9 + 4 g/L glc (glucose minimal media) PGCGROWTHCONDITIONS SRR922262 MCO000002776 M9 + 4 g/L glc ( glucose minimal media ) Medium True
characteristics glucose 2 g/l basal media : <Med> M9 + 4 g/L glc ( glucose minimal media ) </Med> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174826 Mid log_wildtype_glc minimal media + adenine_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 92 51 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json basal media: M9 + 4 g/L glc (glucose minimal media) PGCGROWTHCONDITIONS SRR922263 MCO000002776 M9 + 4 g/L glc ( glucose minimal media ) Medium True
characteristics glucose 2 g/l basal media : <Med> M9 + 4 g/L glc ( glucose minimal media ) </Med> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174827 Mid log_wildtype_glc minimal media + L-tryptophan_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 92 51 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json basal media: M9 + 4 g/L glc (glucose minimal media) PGCGROWTHCONDITIONS SRR922264 MCO000002776 M9 + 4 g/L glc ( glucose minimal media ) Medium True
characteristics glucose 2 g/l basal media : <Med> M9 + 4 g/L glc ( glucose minimal media ) </Med> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174828 Mid log_wildtype_glc minimal media_anaerobic rep1 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 92 51 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json basal media: M9 + 4 g/L glc (glucose minimal media) PGCGROWTHCONDITIONS SRR922265 MCO000002776 M9 + 4 g/L glc ( glucose minimal media ) Medium True
characteristics glucose 2 g/l basal media : <Med> M9 + 4 g/L glc ( glucose minimal media ) </Med> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174829 Mid log_wildtype_glc minimal media_anaerobic rep2 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 92 51 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json basal media: M9 + 4 g/L glc (glucose minimal media) PGCGROWTHCONDITIONS SRR922266 MCO000002776 M9 + 4 g/L glc ( glucose minimal media ) Medium True
characteristics glucose 2 g/l basal media : <Med> M9 + 4 g/L glc ( glucose minimal media ) </Med> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174830 Mid log_wildtype_glc minimal media_anaerobic rep3 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 92 51 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json basal media: M9 + 4 g/L glc (glucose minimal media) PGCGROWTHCONDITIONS SRR922267 MCO000002776 M9 + 4 g/L glc ( glucose minimal media ) Medium True
characteristics glucose 2 g/l basal media : <Med> M9 + 4 g/L glc ( glucose minimal media ) </Med> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174831 Mid log_nac KO_glc minimal media_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 92 51 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json basal media: M9 + 4 g/L glc (glucose minimal media) PGCGROWTHCONDITIONS SRR922268 MCO000002776 M9 + 4 g/L glc ( glucose minimal media ) Medium True
characteristics glucose 2 g/l basal media : <Med> M9 + 4 g/L glc ( glucose minimal media ) </Med> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174832 Mid log_nac KO_glc minimal media + adenine_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 92 51 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json basal media: M9 + 4 g/L glc (glucose minimal media) PGCGROWTHCONDITIONS SRR922269 MCO000002776 M9 + 4 g/L glc ( glucose minimal media ) Medium True
characteristics glucose 2 g/l basal media : <Med> M9 + 4 g/L glc ( glucose minimal media ) </Med> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174833 Mid log_cra KO_glc minimal media_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 92 51 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json basal media: M9 + 4 g/L glc (glucose minimal media) PGCGROWTHCONDITIONS SRR922270 MCO000002776 M9 + 4 g/L glc ( glucose minimal media ) Medium True
characteristics glucose 2 g/l basal media : <Med> M9 + 4 g/L glc ( glucose minimal media ) </Med> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174834 Mid log_cra KO_glc minimal media + L-tryptophan_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 92 51 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json basal media: M9 + 4 g/L glc (glucose minimal media) PGCGROWTHCONDITIONS SRR922271 MCO000002776 M9 + 4 g/L glc ( glucose minimal media ) Medium True
characteristics glucose 2 g/l basal media : <Med> M9 + 4 g/L glc ( glucose minimal media ) </Med> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174835 Mid log_mntR KO_glc minimal media_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 92 51 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json basal media: M9 + 4 g/L glc (glucose minimal media) PGCGROWTHCONDITIONS SRR922272 MCO000002776 M9 + 4 g/L glc ( glucose minimal media ) Medium True
characteristics glucose 2 g/l basal media : <Med> M9 + 4 g/L glc ( glucose minimal media ) </Med> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174836 Mid log_mntR KO_glc minimal media_anaerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 92 51 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json basal media: M9 + 4 g/L glc (glucose minimal media) PGCGROWTHCONDITIONS SRR922273 MCO000002776 M9 + 4 g/L glc ( glucose minimal media ) Medium True
source_name wt <Gtype> WT + </Gtype> <Supp> ade </Supp> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174826 Mid log_wildtype_glc minimal media + adenine_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json WT + ade PGCGROWTHCONDITIONS SRR922263 MCO000002470 WT + Genetic background True
source_name wt <Gtype> WT + </Gtype> <Supp> L-trp </Supp> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174827 Mid log_wildtype_glc minimal media + L-tryptophan_aerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json WT + L-trp PGCGROWTHCONDITIONS SRR922264 MCO000002470 WT + Genetic background True
characteristics stationary phase growth phase : <Phase> stationary </Phase> GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331415 LB 2.0 B1 TEX neg L1 GA 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 77 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: stationary PGCGROWTHCONDITIONS SRR1173971 MCO000002866 stationary Growth phase True
characteristics stationary phase growth phase : <Phase> stationary </Phase> GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331416 LB 2.0 B1 TEX neg L2 HS2 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 77 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: stationary PGCGROWTHCONDITIONS SRR1173972 MCO000002866 stationary Growth phase True
characteristics stationary phase growth phase : <Phase> stationary </Phase> GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331417 LB 2.0 B1 TEX pos L1 GA 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 77 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: stationary PGCGROWTHCONDITIONS SRR1173973 MCO000002866 stationary Growth phase True
characteristics stationary phase growth phase : <Phase> stationary </Phase> GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331418 LB 2.0 B1 TEX pos L2 HS2 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 77 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: stationary PGCGROWTHCONDITIONS SRR1173974 MCO000002866 stationary Growth phase True
characteristics stationary phase growth phase : <Phase> stationary </Phase> GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331419 LB 2.0 B2 TEX neg L1 HS1 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 77 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: stationary PGCGROWTHCONDITIONS SRR1173975 MCO000002866 stationary Growth phase True
characteristics stationary phase growth phase : <Phase> stationary </Phase> GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331420 LB 2.0 B2 TEX neg L1 HS2 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 77 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: stationary PGCGROWTHCONDITIONS SRR1173976 MCO000002866 stationary Growth phase True
characteristics stationary phase growth phase : <Phase> stationary </Phase> GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331421 LB 2.0 B2 TEX neg L2 HS2 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 77 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: stationary PGCGROWTHCONDITIONS SRR1173977 MCO000002866 stationary Growth phase True
characteristics stationary phase growth phase : <Phase> stationary </Phase> GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331422 LB 2.0 B2 TEX pos L1 HS1 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 77 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: stationary PGCGROWTHCONDITIONS SRR1173978 MCO000002866 stationary Growth phase True
characteristics stationary phase growth phase : <Phase> stationary </Phase> GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331423 LB 2.0 B2 TEX pos L1 HS2 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 77 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: stationary PGCGROWTHCONDITIONS SRR1173979 MCO000002866 stationary Growth phase True
characteristics stationary phase growth phase : <Phase> stationary </Phase> GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331424 LB 2.0 B2 TEX pos L2 HS2 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 77 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: stationary PGCGROWTHCONDITIONS SRR1173980 MCO000002866 stationary Growth phase True
characteristics wild type genotype : <Gtype> rne wild-type </Gtype> GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE58285 Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli GSM1405877 rne wild-type 25237058 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE58285/GSE58285.soft.gz 100 82 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: rne wild-type PGCGROWTHCONDITIONS SRR1363864 MCO000002470 rne wild-type Genetic background True
growth_protocol MOPS minimal medium <Gtype> Wild type </Gtype> E. coli K-12 ( strain BW38038 ) and BW39452 ( ΔrpoS ) cultures were grown on <Med> MOPS glucose minimal medium </Med> with <Supp> 0.2 % glucose </Supp> as sole carbon source at <Temp> 37 °C </Temp> , pH was initially 7.4 , and the agitation speed was 500 rpm . Culture samples were harvested during logarithmic growth and following entry into stationary phase for the WT and rpoS mutant . OD600 measurements were made on a Beckman Coulter DU 800 spectrophotometer . Samples were harvested directly into ice-cold RNAlater at a 1:1 dilution to protect RNA from degradation and cells then were pelleted by centrifugation at 8000rpm for 10 minutes . Cell pellets were stored at -80 °C in an equal volume of RNAlater prior to RNA extraction . GPL18814 GPL18814: Illumina HiSeq 2000 (Escherichia coli BW38028) GSE58556 RNA sequencing based analysis of the bacterial transcriptome GSM1413874 WT_glucose_log 25483350 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE58556/GSE58556.soft.gz 100 83 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Wild type E. coli K-12 (strain BW38038) and BW39452(ΔrpoS) cultures were grown on MOPS glucose minimal medium with 0.2% glucose as sole carbon source at 37°C, pH was initially 7.4, and the agitation speed was 500 rpm. Culture samples were harvested during logarithmic growth and following entry into stationary phase for the WT and rpoS mutant. OD600 measurements were made on a Beckman Coulter DU 800 spectrophotometer. Samples were harvested directly into ice-cold RNAlater at a 1:1 dilution to protect RNA from degradation and cells then were pelleted by centrifugation at 8000rpm for 10 minutes. Cell pellets were stored at -80°C in an equal volume of RNAlater prior to RNA extraction. PGCGROWTHCONDITIONS SRR1411272 MCO000002528 MOPS glucose minimal medium Medium True
characteristics log phase treatment : <Phase> log phase sample </Phase> GPL18814 GPL18814: Illumina HiSeq 2000 (Escherichia coli BW38028) GSE58556 RNA sequencing based analysis of the bacterial transcriptome GSM1413874 WT_glucose_log 25483350 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE58556/GSE58556.soft.gz 100 72 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json treatment: log phase sample PGCGROWTHCONDITIONS SRR1411272 MCO000002864 log phase sample Growth phase True
treatment_protocol iptg At OD ~ 0.3 , cultures were induced with <Supp> 1mM IPTG </Supp> for the appropriate length of time . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE58637 MicL, a new σE-dependent sRNA, combats envelope stress by repressing synthesis of Lpp, the major outer membrane lipoprotein GSM1415871 ribosome profiling MicL t0 25030700 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE58637/GSE58637.soft.gz 100 67 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json At OD ~0.3, cultures were induced with 1mM IPTG for the appropriate length of time. PGCGROWTHCONDITIONS SRR1425203 MCO000002105 1mM IPTG Medium supplement True
treatment_protocol iptg At OD ~ 0.3 , cultures were induced with <Supp> 1mM IPTG </Supp> for the appropriate length of time . GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE58637 MicL, a new σE-dependent sRNA, combats envelope stress by repressing synthesis of Lpp, the major outer membrane lipoprotein GSM1415872 ribosome profiling MicL t20 25030700 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE58637/GSE58637.soft.gz 100 67 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json At OD ~0.3, cultures were induced with 1mM IPTG for the appropriate length of time. PGCGROWTHCONDITIONS SRR1425204 MCO000002105 1mM IPTG Medium supplement True
characteristics iptg treatment : <Supp> 1mM IPTG </Supp> GPL14548-GPL21433 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE77617 Operon mRNAs are organized into ORF-centric structures that predict translation efficiency GSM2462936 mRNA-seq 37°C in WT with control plasmid 28139975 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE77617/GSE77617.soft.gz 100 67 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json treatment: 1mM IPTG PGCGROWTHCONDITIONS SRR5186139 MCO000002105 1mM IPTG Medium supplement True
characteristics iptg treatment : <Supp> 1mM IPTG </Supp> GPL14548-GPL21433 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE77617 Operon mRNAs are organized into ORF-centric structures that predict translation efficiency GSM2462937 mRNA-seq 37°C in WT with plasmid expressing mini-ORF CUA 28139975 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE77617/GSE77617.soft.gz 100 67 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json treatment: 1mM IPTG PGCGROWTHCONDITIONS SRR5186140 MCO000002105 1mM IPTG Medium supplement True
characteristics iptg treatment : <Supp> 1mM IPTG </Supp> GPL14548-GPL21433 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE77617 Operon mRNAs are organized into ORF-centric structures that predict translation efficiency GSM2462938 mRNA-seq 37°C in WT with plasmid expressing mini-ORF CUG 28139975 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE77617/GSE77617.soft.gz 100 67 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json treatment: 1mM IPTG PGCGROWTHCONDITIONS SRR5186141 MCO000002105 1mM IPTG Medium supplement True
characteristics wild type genotype : <Gtype> Wild-type </Gtype> GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE60107 Next generation sequencing analysis reveals that the ribonucleases RNase II, RNase R and PNPase affect bacterial motility and biofilm formation in E. coli GSM1465035 WT_RNA-Seq 2575788830486791 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE60107/GSE60107.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: Wild-type PGCGROWTHCONDITIONS SRR1536586 MCO000002470 Wild-type Genetic background True
characteristics mutant genotype : <Gtype> RNase II mutant </Gtype> GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE60107 Next generation sequencing analysis reveals that the ribonucleases RNase II, RNase R and PNPase affect bacterial motility and biofilm formation in E. coli GSM1465036 ∆rnb_RNA-Seq 2575788830486791 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE60107/GSE60107.soft.gz 100 57 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: RNase II mutant PGCGROWTHCONDITIONS SRR1536587 MCO000002469 RNase II mutant Genetic background True
characteristics mutant genotype : <Gtype> RNase R mutant </Gtype> GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE60107 Next generation sequencing analysis reveals that the ribonucleases RNase II, RNase R and PNPase affect bacterial motility and biofilm formation in E. coli GSM1465037 ∆rnr_RNA-Seq 2575788830486791 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE60107/GSE60107.soft.gz 100 60 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: RNase R mutant PGCGROWTHCONDITIONS SRR1536588 MCO000002469 RNase R mutant Genetic background True
characteristics mutant genotype : <Gtype> PNPase mutant </Gtype> GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE60107 Next generation sequencing analysis reveals that the ribonucleases RNase II, RNase R and PNPase affect bacterial motility and biofilm formation in E. coli GSM1465038 ∆pnp_RNA-Seq 2575788830486791 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE60107/GSE60107.soft.gz 100 63 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: PNPase mutant PGCGROWTHCONDITIONS SRR1536589 MCO000002469 PNPase mutant Genetic background True
growth_protocol LB medium <Med> LB medium , </Med> <Temp> 37 °C </Temp> , airated , constant pH 7.5 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE65244 Temporal gene expression in Escherichia coli GSM1590712 Wt – 60 min http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65244/GSE65244.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt LB medium, 37°C, airated, constant pH 7.5 PGCGROWTHCONDITIONS SRR1771414 MCO000002533 LB medium , Medium True
growth_protocol LB medium <Med> LB medium , </Med> <Temp> 37 °C </Temp> , airated , constant pH 7.5 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE65244 Temporal gene expression in Escherichia coli GSM1590717 Fis – 60 min http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65244/GSE65244.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt LB medium, 37°C, airated, constant pH 7.5 PGCGROWTHCONDITIONS SRR1771419 MCO000002533 LB medium , Medium True
growth_protocol LB medium <Med> LB medium , </Med> <Temp> 37 °C </Temp> , airated , constant pH 7.5 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE65244 Temporal gene expression in Escherichia coli GSM1590718 Fis – 120 min http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65244/GSE65244.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt LB medium, 37°C, airated, constant pH 7.5 PGCGROWTHCONDITIONS SRR1771420 MCO000002533 LB medium , Medium True
growth_protocol LB medium <Med> LB medium , </Med> <Temp> 37 °C </Temp> , airated , constant pH 7.5 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE65244 Temporal gene expression in Escherichia coli GSM1590719 Fis – 180 min http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65244/GSE65244.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt LB medium, 37°C, airated, constant pH 7.5 PGCGROWTHCONDITIONS SRR1771421 MCO000002533 LB medium , Medium True
growth_protocol LB medium <Med> LB medium , </Med> <Temp> 37 °C </Temp> , airated , constant pH 7.5 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE65244 Temporal gene expression in Escherichia coli GSM1590720 Fis – 420 min http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65244/GSE65244.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt LB medium, 37°C, airated, constant pH 7.5 PGCGROWTHCONDITIONS SRR1771422 MCO000002533 LB medium , Medium True
growth_protocol LB medium <Med> LB medium , </Med> <Temp> 37 °C </Temp> , airated , constant pH 7.5 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE65244 Temporal gene expression in Escherichia coli GSM1590721 Hns – 60 min http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65244/GSE65244.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt LB medium, 37°C, airated, constant pH 7.5 PGCGROWTHCONDITIONS SRR1771423 MCO000002533 LB medium , Medium True
growth_protocol LB medium <Med> LB medium , </Med> <Temp> 37 °C </Temp> , airated , constant pH 7.5 GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE65244 Temporal gene expression in Escherichia coli GSM1590722 Hns – 120 min http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65244/GSE65244.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt LB medium, 37°C, airated, constant pH 7.5 PGCGROWTHCONDITIONS SRR1771424 MCO000002533 LB medium , Medium True
characteristics paraquat 250 µM treated with : <Supp> 250 uM of paraquat </Supp> at mid-log pahse for <Supp> 20 min </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603386 WT PQ 1 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 92 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treated with: 250 uM of paraquat at mid-log pahse for 20 min PGCGROWTHCONDITIONS SRR1796598 MCO000011582 250 uM of paraquat Medium supplement True
characteristics paraquat 250 µM treated with : <Supp> 250 uM of paraquat </Supp> at mid-log pahse for <Supp> 20 min </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603387 WT PQ 2 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 92 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treated with: 250 uM of paraquat at mid-log pahse for 20 min PGCGROWTHCONDITIONS SRR1796599 MCO000011582 250 uM of paraquat Medium supplement True
characteristics paraquat 250 µM treated with : <Supp> 250 uM of paraquat </Supp> at mid-log pahse for <Supp> 20 min </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603388 ΔoxyR PQ 1 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 92 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treated with: 250 uM of paraquat at mid-log pahse for 20 min PGCGROWTHCONDITIONS SRR1796600 MCO000011582 250 uM of paraquat Medium supplement True
characteristics paraquat 250 µM treated with : <Supp> 250 uM of paraquat </Supp> at mid-log pahse for <Supp> 20 min </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603389 ΔoxyR PQ 2 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 92 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treated with: 250 uM of paraquat at mid-log pahse for 20 min PGCGROWTHCONDITIONS SRR1796601 MCO000011582 250 uM of paraquat Medium supplement True
characteristics paraquat 250 µM treated with : <Supp> 250 uM of paraquat </Supp> at mid-log pahse for <Supp> 20 min </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603391 ΔsoxR PQ 2 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 92 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treated with: 250 uM of paraquat at mid-log pahse for 20 min PGCGROWTHCONDITIONS SRR1796603 MCO000011582 250 uM of paraquat Medium supplement True
characteristics paraquat 250 µM treated with : <Supp> 250 uM of paraquat </Supp> at mid-log pahse for <Supp> 20 min </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603392 ΔsoxS PQ 1 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 92 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treated with: 250 uM of paraquat at mid-log pahse for 20 min PGCGROWTHCONDITIONS SRR1796604 MCO000011582 250 uM of paraquat Medium supplement True
characteristics paraquat 250 µM treated with : <Supp> 250 uM of paraquat </Supp> at mid-log pahse for <Supp> 20 min </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65711 Genome-wide OxyR and SoxRS transcriptional regulatory networks coordinate complex cellular responses to oxidative stress [RNA-seq] GSM1603393 ΔsoxS PQ 2 26279566 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65711/GSE65711.soft.gz 92 81 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treated with: 250 uM of paraquat at mid-log pahse for 20 min PGCGROWTHCONDITIONS SRR1796605 MCO000011582 250 uM of paraquat Medium supplement True
characteristics OD600 of 0.3 growth phase : <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623160 WT pH5.5 1 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 86 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: mid-log phase (OD600 = 0.3) PGCGROWTHCONDITIONS SRR1824557 MCO000002564 OD600 = 0.3 Optical Density (OD) True
characteristics OD600 of 0.3 growth phase : <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623161 WT pH5.5 2 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 86 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: mid-log phase (OD600 = 0.3) PGCGROWTHCONDITIONS SRR1824558 MCO000002564 OD600 = 0.3 Optical Density (OD) True
characteristics OD600 of 0.3 growth phase : <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623162 ΔgadE pH5.5 1 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 86 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: mid-log phase (OD600 = 0.3) PGCGROWTHCONDITIONS SRR1824559 MCO000002564 OD600 = 0.3 Optical Density (OD) True
characteristics OD600 of 0.3 growth phase : <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623163 ΔgadE pH5.5 2 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 86 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: mid-log phase (OD600 = 0.3) PGCGROWTHCONDITIONS SRR1824560 MCO000002564 OD600 = 0.3 Optical Density (OD) True
characteristics OD600 of 0.3 growth phase : <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623164 ΔgadW pH5.5 1 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 86 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: mid-log phase (OD600 = 0.3) PGCGROWTHCONDITIONS SRR1824561 MCO000002564 OD600 = 0.3 Optical Density (OD) True
characteristics OD600 of 0.3 growth phase : <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623165 ΔgadW pH5.5 2 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 86 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: mid-log phase (OD600 = 0.3) PGCGROWTHCONDITIONS SRR1824562 MCO000002564 OD600 = 0.3 Optical Density (OD) True
characteristics OD600 of 0.3 growth phase : <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623166 ΔgadX pH5.5 1 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 86 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: mid-log phase (OD600 = 0.3) PGCGROWTHCONDITIONS SRR1824563 MCO000002564 OD600 = 0.3 Optical Density (OD) True
characteristics OD600 of 0.3 growth phase : <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623167 ΔgadX pH5.5 2 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 86 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: mid-log phase (OD600 = 0.3) PGCGROWTHCONDITIONS SRR1824564 MCO000002564 OD600 = 0.3 Optical Density (OD) True
characteristics T cell <Gtype> cell type </Gtype> : Escherichia coli str . <Strain> K-12 </Strain> substr . <Substrain> MG1655 </Substrain> GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE67218 Transcriptional expression level of E. coli at 37 ℃ in LB media GSM1642593 RNA-seq 37C LB rep1 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE67218/GSE67218.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt cell type: Escherichia coli str. K-12 substr. MG1655 PGCGROWTHCONDITIONS SRR1927169 MCO000011472 cell type Genetic background True
characteristics T cell <Gtype> cell type </Gtype> : Escherichia coli str . <Strain> K-12 </Strain> substr . <Substrain> MG1655 </Substrain> GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE67218 Transcriptional expression level of E. coli at 37 ℃ in LB media GSM1642594 RNA-seq 37C LB rep2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE67218/GSE67218.soft.gz 80 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt cell type: Escherichia coli str. K-12 substr. MG1655 PGCGROWTHCONDITIONS SRR1927170 MCO000011472 cell type Genetic background True
characteristics glucose 0.5% treatment : <Supp> 0.3 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900470 Parent LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.3% glucose PGCGROWTHCONDITIONS SRR2547467 MCO000002772 0.3 % glucose Medium supplement True
characteristics glucose 0.5% treatment : <Supp> 0.3 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900472 cysG KO LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.3% glucose PGCGROWTHCONDITIONS SRR2547469 MCO000002772 0.3 % glucose Medium supplement True
characteristics glucose 0.5% treatment : <Supp> 0.3 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900474 cysH KO LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.3% glucose PGCGROWTHCONDITIONS SRR2547471 MCO000002772 0.3 % glucose Medium supplement True
characteristics glucose 0.5% treatment : <Supp> 0.3 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900475 cysH KO LB rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.3% glucose PGCGROWTHCONDITIONS SRR2547472 MCO000002772 0.3 % glucose Medium supplement True
characteristics glucose 0.5% treatment : <Supp> 0.3 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900476 dcd KO LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.3% glucose PGCGROWTHCONDITIONS SRR2547473 MCO000002772 0.3 % glucose Medium supplement True
characteristics glucose 0.5% treatment : <Supp> 0.3 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900477 dcd KO LB rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.3% glucose PGCGROWTHCONDITIONS SRR2547474 MCO000002772 0.3 % glucose Medium supplement True
characteristics glucose 0.5% treatment : <Supp> 0.3 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900478 fadr KO LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.3% glucose PGCGROWTHCONDITIONS SRR2547475 MCO000002772 0.3 % glucose Medium supplement True
characteristics glucose 0.5% treatment : <Supp> 0.3 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900479 fadr KO LB rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.3% glucose PGCGROWTHCONDITIONS SRR2547476 MCO000002772 0.3 % glucose Medium supplement True
characteristics glucose 0.5% treatment : <Supp> 0.3 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900480 ppk KO LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.3% glucose PGCGROWTHCONDITIONS SRR2547477 MCO000002772 0.3 % glucose Medium supplement True
characteristics glucose 0.5% treatment : <Supp> 0.3 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900482 wzc KO LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.3% glucose PGCGROWTHCONDITIONS SRR2547479 MCO000002772 0.3 % glucose Medium supplement True
characteristics glucose 0.5% treatment : <Supp> 0.3 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900483 wzc KO LB rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.3% glucose PGCGROWTHCONDITIONS SRR2547480 MCO000002772 0.3 % glucose Medium supplement True
characteristics glucose 0.5% treatment : <Supp> 0.3 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900485 yghD KO LB rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.3% glucose PGCGROWTHCONDITIONS SRR2547482 MCO000002772 0.3 % glucose Medium supplement True
characteristics glucose 0.5% treatment : <Supp> 0.3 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900486 fepA KO LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.3% glucose PGCGROWTHCONDITIONS SRR2547483 MCO000002772 0.3 % glucose Medium supplement True
characteristics glucose 0.5% treatment : <Supp> 0.3 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900488 lacA KO LB rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.3% glucose PGCGROWTHCONDITIONS SRR2547485 MCO000002772 0.3 % glucose Medium supplement True
characteristics glucose 0.5% treatment : <Supp> 0.3 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900490 Parent M9 rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.3% glucose PGCGROWTHCONDITIONS SRR2547487 MCO000002772 0.3 % glucose Medium supplement True
characteristics glucose 0.5% treatment : <Supp> 0.3 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900491 Parent M9 rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.3% glucose PGCGROWTHCONDITIONS SRR2547488 MCO000002772 0.3 % glucose Medium supplement True
characteristics glucose 0.5% treatment : <Supp> 0.3 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900492 dcd KO M9 rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.3% glucose PGCGROWTHCONDITIONS SRR2547489 MCO000002772 0.3 % glucose Medium supplement True
characteristics glucose 0.5% treatment : <Supp> 0.3 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900493 dcd KO M9 rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.3% glucose PGCGROWTHCONDITIONS SRR2547490 MCO000002772 0.3 % glucose Medium supplement True
characteristics glucose 0.5% treatment : <Supp> 0.3 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900494 fadr KO M9 rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.3% glucose PGCGROWTHCONDITIONS SRR2547491 MCO000002772 0.3 % glucose Medium supplement True
characteristics glucose 0.5% treatment : <Supp> 0.3 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900495 fadr KO M9 rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.3% glucose PGCGROWTHCONDITIONS SRR2547492 MCO000002772 0.3 % glucose Medium supplement True
characteristics glucose 0.5% treatment : <Supp> 0.3 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900496 ppk KO M9 rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.3% glucose PGCGROWTHCONDITIONS SRR2547493 MCO000002772 0.3 % glucose Medium supplement True
characteristics glucose 0.5% treatment : <Supp> 0.3 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900497 ppk KO M9 rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.3% glucose PGCGROWTHCONDITIONS SRR2547494 MCO000002772 0.3 % glucose Medium supplement True
characteristics glucose 0.5% treatment : <Supp> 0.3 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900498 wzc KO M9 rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.3% glucose PGCGROWTHCONDITIONS SRR2547495 MCO000002772 0.3 % glucose Medium supplement True
characteristics glucose 0.5% treatment : <Supp> 0.3 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900499 wzc KO M9 rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.3% glucose PGCGROWTHCONDITIONS SRR2547496 MCO000002772 0.3 % glucose Medium supplement True
characteristics glucose 0.5% treatment : <Supp> 0.3 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900500 yghD KO M9 rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.3% glucose PGCGROWTHCONDITIONS SRR2547497 MCO000002772 0.3 % glucose Medium supplement True
characteristics glucose 0.5% treatment : <Supp> 0.3 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900501 yghD KO M9 rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.3% glucose PGCGROWTHCONDITIONS SRR2547498 MCO000002772 0.3 % glucose Medium supplement True
characteristics glucose 0.5% treatment : <Supp> 0.3 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900502 fepA KO M9 rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 91 91 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.3% glucose PGCGROWTHCONDITIONS SRR2547499 MCO000002772 0.3 % glucose Medium supplement True
source_name glucose Whole cell , WT , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900507 WT rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, WT, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547504 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , mgtA KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900509 mgtA KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, mgtA KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547506 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , mgtA KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900510 mgtA KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, mgtA KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547507 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , gabT KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900513 gabT KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, gabT KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547510 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , gabT KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900514 gabT KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, gabT KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547511 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , sdhC KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900515 sdhC KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, sdhC KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547512 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , sdhC KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900516 sdhC KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, sdhC KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547513 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , putP KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900518 putP KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, putP KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547515 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , putP KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900520 putP KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, putP KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547517 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , rfbA KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900521 rfbA KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, rfbA KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547518 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , rfbA KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900522 rfbA KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, rfbA KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547519 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , entF KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900524 entF KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, entF KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547521 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , entF KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900525 entF KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, entF KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547522 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , entF KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900526 entF KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, entF KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547523 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , kefB KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900527 kefB KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, kefB KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547524 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , kefB KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900529 kefB KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, kefB KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547526 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , cysA KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900530 cysA KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, cysA KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547527 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , cysA KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900531 cysA KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, cysA KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547528 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , cysA KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900532 cysA KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, cysA KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547529 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , galE KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900534 galE KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, galE KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547531 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , mhpD KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900536 mhpD KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, mhpD KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547533 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , mhpD KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900537 mhpD KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, mhpD KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547534 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , mhpD KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900538 mhpD KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, mhpD KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547535 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , fliY KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900539 fliY KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, fliY KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547536 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , fliY KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900540 fliY KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, fliY KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547537 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , fliY KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900541 fliY KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, fliY KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547538 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , lplA KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900542 lplA KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, lplA KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547539 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , lplA KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900543 lplA KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, lplA KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547540 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , lplA KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900544 lplA KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, lplA KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547541 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , khc KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900545 khc KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, khc KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547542 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , khc KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900546 khc KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, khc KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547543 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , khc KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900547 khc KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, khc KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547544 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , ugpC KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900548 ugpC KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, ugpC KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547545 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , ugpC KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900549 ugpC KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, ugpC KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547546 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , ugpC KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900550 ugpC KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, ugpC KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547547 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , trpD KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900551 trpD KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, trpD KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547548 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , trpD KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900552 trpD KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, trpD KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547549 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , trpD KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900553 trpD KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, trpD KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547550 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , aspC KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900554 aspC KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, aspC KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547551 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , aspC KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900555 aspC KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, aspC KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547552 MCO000000245 % glucose Medium supplement True
source_name glucose Whole cell , aspC KO , M9 and 0.4 <Supp> % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900556 aspC KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Whole cell, aspC KO, M9 and 0.4% glucose PGCGROWTHCONDITIONS SRR2547553 MCO000000245 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900507 WT rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547504 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900509 mgtA KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547506 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900510 mgtA KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547507 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900513 gabT KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547510 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900514 gabT KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547511 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900515 sdhC KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547512 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900516 sdhC KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547513 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900518 putP KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547515 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900520 putP KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547517 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900521 rfbA KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547518 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900522 rfbA KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547519 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900524 entF KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547521 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900525 entF KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547522 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900526 entF KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547523 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900527 kefB KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547524 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900529 kefB KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547526 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900530 cysA KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547527 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900531 cysA KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547528 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900532 cysA KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547529 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900534 galE KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547531 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900536 mhpD KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547533 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900537 mhpD KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547534 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900538 mhpD KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547535 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900539 fliY KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547536 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900540 fliY KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547537 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900541 fliY KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547538 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900542 lplA KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547539 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900543 lplA KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547540 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900544 lplA KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547541 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900545 khc KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547542 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900546 khc KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547543 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900547 khc KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547544 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900548 ugpC KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547545 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900549 ugpC KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547546 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900550 ugpC KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547547 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900551 trpD KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547548 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900552 trpD KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547549 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900553 trpD KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547550 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900554 aspC KO rep1 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547551 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900555 aspC KO rep2 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547552 MCO000002771 0.4 % glucose Medium supplement True
characteristics glucose 0.4% treatment : <Supp> 0.4 % glucose </Supp> GPL20227 GPL20227: Illumina HiSeq 2500 (Escherichia coli BW25113) GSE73672 Targeted experimentation to increase functional coverage in omics dataset GSM1900556 aspC KO rep3 27713404 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73672/GSE73672.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: 0.4% glucose PGCGROWTHCONDITIONS SRR2547553 MCO000002771 0.4 % glucose Medium supplement True
growth_protocol OD600 of 0.8 Overnight cultures of bacteria were diluted 1:100 in fresh LB broth and grown to logarithmic phase at an <OD> OD600 of about 0.8 </OD> . GPL21021 GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933) GSE73969 RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933 GSM1906887 wt_1 2717363528288207 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73969/GSE73969.soft.gz 100 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Overnight cultures of bacteria were diluted 1:100 in fresh LB broth and grown to logarithmic phase at an OD600 of about 0.8. PGCGROWTHCONDITIONS SRR2637695 MCO000003177 OD600 of about 0.8 Optical Density (OD) True
growth_protocol OD600 of 0.8 Overnight cultures of bacteria were diluted 1:100 in fresh LB broth and grown to logarithmic phase at an <OD> OD600 of about 0.8 </OD> . GPL21021 GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933) GSE73969 RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933 GSM1906888 wt_2 2717363528288207 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73969/GSE73969.soft.gz 100 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Overnight cultures of bacteria were diluted 1:100 in fresh LB broth and grown to logarithmic phase at an OD600 of about 0.8. PGCGROWTHCONDITIONS SRR2637696 MCO000003177 OD600 of about 0.8 Optical Density (OD) True
growth_protocol OD600 of 0.8 Overnight cultures of bacteria were diluted 1:100 in fresh LB broth and grown to logarithmic phase at an <OD> OD600 of about 0.8 </OD> . GPL21021 GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933) GSE73969 RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933 GSM1906889 hns_1 2717363528288207 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73969/GSE73969.soft.gz 100 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Overnight cultures of bacteria were diluted 1:100 in fresh LB broth and grown to logarithmic phase at an OD600 of about 0.8. PGCGROWTHCONDITIONS SRR2637697 MCO000003177 OD600 of about 0.8 Optical Density (OD) True
growth_protocol OD600 of 0.8 Overnight cultures of bacteria were diluted 1:100 in fresh LB broth and grown to logarithmic phase at an <OD> OD600 of about 0.8 </OD> . GPL21021 GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933) GSE73969 RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933 GSM1906890 hns_2 2717363528288207 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73969/GSE73969.soft.gz 100 80 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Overnight cultures of bacteria were diluted 1:100 in fresh LB broth and grown to logarithmic phase at an OD600 of about 0.8. PGCGROWTHCONDITIONS SRR2637698 MCO000003177 OD600 of about 0.8 Optical Density (OD) True
growth_protocol glucose 0.5% M9 defined medium ( 0.6 % Na2HPO4 , 0.3 % KH2PO4 , 0.05 % NaCl , 0.01 % NH4Cl , 0.1 mM CaCl2 , 1 mM MgSO4 , 5 x 10 − 4 % Thiamin ) supplemented with <Supp> 0.5 % glucose </Supp> and 0.1 % amino acids was used for RNA-seq experiments . GPL15982 GPL15982: Illumina HiSeq 1000 (Escherichia coli str. K-12 substr. MG1655) GSE74809 Growth-phase dependent regulation of transcription by Rsd and 6S RNA GSM1933982 rpoS_TS_1 29686109 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE74809/GSE74809.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt M9 defined medium (0.6% Na2HPO4, 0.3% KH2PO4, 0.05% NaCl, 0.01% NH4Cl, 0.1 mM CaCl2, 1 mM MgSO4, 5 x 10−4% Thiamin) supplemented with 0.5% glucose and 0.1% amino acids was used for RNA-seq experiments. PGCGROWTHCONDITIONS SRR2932665 MCO000002772 0.5 % glucose Medium supplement True
growth_protocol glucose 0.5% M9 defined medium ( 0.6 % Na2HPO4 , 0.3 % KH2PO4 , 0.05 % NaCl , 0.01 % NH4Cl , 0.1 mM CaCl2 , 1 mM MgSO4 , 5 x 10 − 4 % Thiamin ) supplemented with <Supp> 0.5 % glucose </Supp> and 0.1 % amino acids was used for RNA-seq experiments . GPL15982 GPL15982: Illumina HiSeq 1000 (Escherichia coli str. K-12 substr. MG1655) GSE74809 Growth-phase dependent regulation of transcription by Rsd and 6S RNA GSM1933999 ssrS_LS_2 29686109 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE74809/GSE74809.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt M9 defined medium (0.6% Na2HPO4, 0.3% KH2PO4, 0.05% NaCl, 0.01% NH4Cl, 0.1 mM CaCl2, 1 mM MgSO4, 5 x 10−4% Thiamin) supplemented with 0.5% glucose and 0.1% amino acids was used for RNA-seq experiments. PGCGROWTHCONDITIONS SRR2932682 MCO000002772 0.5 % glucose Medium supplement True
characteristics stationary phase growth phase : <Gtype> Late Stationary </Gtype> GPL15982 GPL15982: Illumina HiSeq 1000 (Escherichia coli str. K-12 substr. MG1655) GSE74809 Growth-phase dependent regulation of transcription by Rsd and 6S RNA GSM1933999 ssrS_LS_2 29686109 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE74809/GSE74809.soft.gz 84 84 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth phase: Late Stationary PGCGROWTHCONDITIONS SRR2932682 MCO000002866 Late Stationary Genetic background True
growth_protocol nicl2 5 µm Transcriptomic analyses were carried out after growing bacteria in minimal media supplemented with <Supp> glucose </Supp> . Several Ni concentrations and exposure times were assayed . rcnA gene expression was taken as an internal control to arbitrate between the different conditions . rcnA is induced by Ni when cells are overloaded with this ion and must detoxify the cytoplasm by extruding excess metal via the RcnAB efflux system . rcnA induction was maximised after culture incubation for 10 min , and longer periods of incubation lead to a decline in rcnA expression ( Fig . S1 ) . For the RNA-Seq experiments , bacteria were grown until O.D600nm = 0.3 , were treated with <Supp> 50 µM NiCl2 </Supp> for 10 min and were frozen prior to RNA extraction . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE76167 Mechanisms of nickel toxicity in bacteria GSM1975603 Rodrigue_1-WT-phiNi-1 2737513027668277 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE76167/GSE76167.soft.gz 95 95 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Transcriptomic analyses were carried out after growing bacteria in minimal media supplemented with glucose. Several Ni concentrations and exposure times were assayed. rcnA gene expression was taken as an internal control to arbitrate between the different conditions. rcnA is induced by Ni when cells are overloaded with this ion and must detoxify the cytoplasm by extruding excess metal via the RcnAB efflux system. rcnA induction was maximised after culture incubation for 10 min, and longer periods of incubation lead to a decline in rcnA expression (Fig. S1). For the RNA-Seq experiments, bacteria were grown until O.D600nm = 0.3, were treated with 50 µM NiCl2 for 10 min and were frozen prior to RNA extraction. PGCGROWTHCONDITIONS SRR3033275SRR3033276SRR3033277SRR3033278SRR3033279 MCO000002790 50 µM NiCl2 Medium supplement True
growth_protocol nicl2 5 µm Transcriptomic analyses were carried out after growing bacteria in minimal media supplemented with <Supp> glucose </Supp> . Several Ni concentrations and exposure times were assayed . rcnA gene expression was taken as an internal control to arbitrate between the different conditions . rcnA is induced by Ni when cells are overloaded with this ion and must detoxify the cytoplasm by extruding excess metal via the RcnAB efflux system . rcnA induction was maximised after culture incubation for 10 min , and longer periods of incubation lead to a decline in rcnA expression ( Fig . S1 ) . For the RNA-Seq experiments , bacteria were grown until O.D600nm = 0.3 , were treated with <Supp> 50 µM NiCl2 </Supp> for 10 min and were frozen prior to RNA extraction . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE76167 Mechanisms of nickel toxicity in bacteria GSM1975604 Rodrigue_6-WT-phiNi-2 2737513027668277 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE76167/GSE76167.soft.gz 95 95 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Transcriptomic analyses were carried out after growing bacteria in minimal media supplemented with glucose. Several Ni concentrations and exposure times were assayed. rcnA gene expression was taken as an internal control to arbitrate between the different conditions. rcnA is induced by Ni when cells are overloaded with this ion and must detoxify the cytoplasm by extruding excess metal via the RcnAB efflux system. rcnA induction was maximised after culture incubation for 10 min, and longer periods of incubation lead to a decline in rcnA expression (Fig. S1). For the RNA-Seq experiments, bacteria were grown until O.D600nm = 0.3, were treated with 50 µM NiCl2 for 10 min and were frozen prior to RNA extraction. PGCGROWTHCONDITIONS SRR3033280SRR3033281SRR3033282SRR3033283 MCO000002790 50 µM NiCl2 Medium supplement True
growth_protocol nicl2 5 µm Transcriptomic analyses were carried out after growing bacteria in minimal media supplemented with <Supp> glucose </Supp> . Several Ni concentrations and exposure times were assayed . rcnA gene expression was taken as an internal control to arbitrate between the different conditions . rcnA is induced by Ni when cells are overloaded with this ion and must detoxify the cytoplasm by extruding excess metal via the RcnAB efflux system . rcnA induction was maximised after culture incubation for 10 min , and longer periods of incubation lead to a decline in rcnA expression ( Fig . S1 ) . For the RNA-Seq experiments , bacteria were grown until O.D600nm = 0.3 , were treated with <Supp> 50 µM NiCl2 </Supp> for 10 min and were frozen prior to RNA extraction . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE76167 Mechanisms of nickel toxicity in bacteria GSM1975605 Rodrigue_9-WT-phiNi-3 2737513027668277 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE76167/GSE76167.soft.gz 95 95 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Transcriptomic analyses were carried out after growing bacteria in minimal media supplemented with glucose. Several Ni concentrations and exposure times were assayed. rcnA gene expression was taken as an internal control to arbitrate between the different conditions. rcnA is induced by Ni when cells are overloaded with this ion and must detoxify the cytoplasm by extruding excess metal via the RcnAB efflux system. rcnA induction was maximised after culture incubation for 10 min, and longer periods of incubation lead to a decline in rcnA expression (Fig. S1). For the RNA-Seq experiments, bacteria were grown until O.D600nm = 0.3, were treated with 50 µM NiCl2 for 10 min and were frozen prior to RNA extraction. PGCGROWTHCONDITIONS SRR3033284SRR3033285SRR3033286SRR3033287 MCO000002790 50 µM NiCl2 Medium supplement True
growth_protocol nicl2 5 µm Transcriptomic analyses were carried out after growing bacteria in minimal media supplemented with <Supp> glucose </Supp> . Several Ni concentrations and exposure times were assayed . rcnA gene expression was taken as an internal control to arbitrate between the different conditions . rcnA is induced by Ni when cells are overloaded with this ion and must detoxify the cytoplasm by extruding excess metal via the RcnAB efflux system . rcnA induction was maximised after culture incubation for 10 min , and longer periods of incubation lead to a decline in rcnA expression ( Fig . S1 ) . For the RNA-Seq experiments , bacteria were grown until O.D600nm = 0.3 , were treated with <Supp> 50 µM NiCl2 </Supp> for 10 min and were frozen prior to RNA extraction . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE76167 Mechanisms of nickel toxicity in bacteria GSM1975606 Rodrigue_2-WT-Ni-1 2737513027668277 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE76167/GSE76167.soft.gz 95 95 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Transcriptomic analyses were carried out after growing bacteria in minimal media supplemented with glucose. Several Ni concentrations and exposure times were assayed. rcnA gene expression was taken as an internal control to arbitrate between the different conditions. rcnA is induced by Ni when cells are overloaded with this ion and must detoxify the cytoplasm by extruding excess metal via the RcnAB efflux system. rcnA induction was maximised after culture incubation for 10 min, and longer periods of incubation lead to a decline in rcnA expression (Fig. S1). For the RNA-Seq experiments, bacteria were grown until O.D600nm = 0.3, were treated with 50 µM NiCl2 for 10 min and were frozen prior to RNA extraction. PGCGROWTHCONDITIONS SRR3033288SRR3033289SRR3033290SRR3033291SRR3033292 MCO000002790 50 µM NiCl2 Medium supplement True
growth_protocol nicl2 5 µm Transcriptomic analyses were carried out after growing bacteria in minimal media supplemented with <Supp> glucose </Supp> . Several Ni concentrations and exposure times were assayed . rcnA gene expression was taken as an internal control to arbitrate between the different conditions . rcnA is induced by Ni when cells are overloaded with this ion and must detoxify the cytoplasm by extruding excess metal via the RcnAB efflux system . rcnA induction was maximised after culture incubation for 10 min , and longer periods of incubation lead to a decline in rcnA expression ( Fig . S1 ) . For the RNA-Seq experiments , bacteria were grown until O.D600nm = 0.3 , were treated with <Supp> 50 µM NiCl2 </Supp> for 10 min and were frozen prior to RNA extraction . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE76167 Mechanisms of nickel toxicity in bacteria GSM1975607 Rodrigue_5-WT-Ni-2 2737513027668277 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE76167/GSE76167.soft.gz 95 95 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Transcriptomic analyses were carried out after growing bacteria in minimal media supplemented with glucose. Several Ni concentrations and exposure times were assayed. rcnA gene expression was taken as an internal control to arbitrate between the different conditions. rcnA is induced by Ni when cells are overloaded with this ion and must detoxify the cytoplasm by extruding excess metal via the RcnAB efflux system. rcnA induction was maximised after culture incubation for 10 min, and longer periods of incubation lead to a decline in rcnA expression (Fig. S1). For the RNA-Seq experiments, bacteria were grown until O.D600nm = 0.3, were treated with 50 µM NiCl2 for 10 min and were frozen prior to RNA extraction. PGCGROWTHCONDITIONS SRR3033293SRR3033294SRR3033295SRR3033296 MCO000002790 50 µM NiCl2 Medium supplement True
growth_protocol nicl2 5 µm Transcriptomic analyses were carried out after growing bacteria in minimal media supplemented with <Supp> glucose </Supp> . Several Ni concentrations and exposure times were assayed . rcnA gene expression was taken as an internal control to arbitrate between the different conditions . rcnA is induced by Ni when cells are overloaded with this ion and must detoxify the cytoplasm by extruding excess metal via the RcnAB efflux system . rcnA induction was maximised after culture incubation for 10 min , and longer periods of incubation lead to a decline in rcnA expression ( Fig . S1 ) . For the RNA-Seq experiments , bacteria were grown until O.D600nm = 0.3 , were treated with <Supp> 50 µM NiCl2 </Supp> for 10 min and were frozen prior to RNA extraction . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE76167 Mechanisms of nickel toxicity in bacteria GSM1975608 Rodrigue_10-WT-Ni-3 2737513027668277 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE76167/GSE76167.soft.gz 95 95 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Transcriptomic analyses were carried out after growing bacteria in minimal media supplemented with glucose. Several Ni concentrations and exposure times were assayed. rcnA gene expression was taken as an internal control to arbitrate between the different conditions. rcnA is induced by Ni when cells are overloaded with this ion and must detoxify the cytoplasm by extruding excess metal via the RcnAB efflux system. rcnA induction was maximised after culture incubation for 10 min, and longer periods of incubation lead to a decline in rcnA expression (Fig. S1). For the RNA-Seq experiments, bacteria were grown until O.D600nm = 0.3, were treated with 50 µM NiCl2 for 10 min and were frozen prior to RNA extraction. PGCGROWTHCONDITIONS SRR3033297SRR3033298SRR3033299SRR3033300SRR3033301 MCO000002790 50 µM NiCl2 Medium supplement True
growth_protocol OD600 of 0.3 E. coli strain NM580 ( genotype MG1655 ermBL-ermB ' : <Gtype> : LacZ </Gtype> ) cells were grown in LB broth ( <Temp> 37 °C </Temp> ) until <OD> OD600 of ~ 0.3 </OD> . GPL21726 GPL21726: Ion Torrent Proton (Escherichia coli) GSE80251 Genome-wide transcriptome analyses of E. coli treated with ribosome-targeting antibiotics GSM2122743 Untreated_replicate_1 27645242 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80251/GSE80251.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli strain NM580 (genotype MG1655 ermBL-ermB’::LacZ) cells were grown in LB broth (37°C) until OD600 of ~0.3. PGCGROWTHCONDITIONS SRR3379590 MCO000002564 OD600 of ~ 0.3 Optical Density (OD) True
growth_protocol OD600 of 0.3 E. coli strain NM580 ( genotype MG1655 ermBL-ermB ' : <Gtype> : LacZ </Gtype> ) cells were grown in LB broth ( <Temp> 37 °C </Temp> ) until <OD> OD600 of ~ 0.3 </OD> . GPL21726 GPL21726: Ion Torrent Proton (Escherichia coli) GSE80251 Genome-wide transcriptome analyses of E. coli treated with ribosome-targeting antibiotics GSM2122744 Untreated_replicate_2 27645242 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80251/GSE80251.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli strain NM580 (genotype MG1655 ermBL-ermB’::LacZ) cells were grown in LB broth (37°C) until OD600 of ~0.3. PGCGROWTHCONDITIONS SRR3379591 MCO000002564 OD600 of ~ 0.3 Optical Density (OD) True
growth_protocol OD600 of 0.3 E. coli strain NM580 ( genotype MG1655 ermBL-ermB ' : <Gtype> : LacZ </Gtype> ) cells were grown in LB broth ( <Temp> 37 °C </Temp> ) until <OD> OD600 of ~ 0.3 </OD> . GPL21726 GPL21726: Ion Torrent Proton (Escherichia coli) GSE80251 Genome-wide transcriptome analyses of E. coli treated with ribosome-targeting antibiotics GSM2122745 Untreated_replicate_3 27645242 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80251/GSE80251.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli strain NM580 (genotype MG1655 ermBL-ermB’::LacZ) cells were grown in LB broth (37°C) until OD600 of ~0.3. PGCGROWTHCONDITIONS SRR3379592 MCO000002564 OD600 of ~ 0.3 Optical Density (OD) True
growth_protocol OD600 of 0.3 E. coli strain NM580 ( genotype MG1655 ermBL-ermB ' : <Gtype> : LacZ </Gtype> ) cells were grown in LB broth ( <Temp> 37 °C </Temp> ) until <OD> OD600 of ~ 0.3 </OD> . GPL21726 GPL21726: Ion Torrent Proton (Escherichia coli) GSE80251 Genome-wide transcriptome analyses of E. coli treated with ribosome-targeting antibiotics GSM2122746 Erythromycin_replicate_1 27645242 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80251/GSE80251.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli strain NM580 (genotype MG1655 ermBL-ermB’::LacZ) cells were grown in LB broth (37°C) until OD600 of ~0.3. PGCGROWTHCONDITIONS SRR3379593 MCO000002564 OD600 of ~ 0.3 Optical Density (OD) True
growth_protocol OD600 of 0.3 E. coli strain NM580 ( genotype MG1655 ermBL-ermB ' : <Gtype> : LacZ </Gtype> ) cells were grown in LB broth ( <Temp> 37 °C </Temp> ) until <OD> OD600 of ~ 0.3 </OD> . GPL21726 GPL21726: Ion Torrent Proton (Escherichia coli) GSE80251 Genome-wide transcriptome analyses of E. coli treated with ribosome-targeting antibiotics GSM2122747 Erythromycin_replicate_2 27645242 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80251/GSE80251.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli strain NM580 (genotype MG1655 ermBL-ermB’::LacZ) cells were grown in LB broth (37°C) until OD600 of ~0.3. PGCGROWTHCONDITIONS SRR3379594 MCO000002564 OD600 of ~ 0.3 Optical Density (OD) True
growth_protocol OD600 of 0.3 E. coli strain NM580 ( genotype MG1655 ermBL-ermB ' : <Gtype> : LacZ </Gtype> ) cells were grown in LB broth ( <Temp> 37 °C </Temp> ) until <OD> OD600 of ~ 0.3 </OD> . GPL21726 GPL21726: Ion Torrent Proton (Escherichia coli) GSE80251 Genome-wide transcriptome analyses of E. coli treated with ribosome-targeting antibiotics GSM2122748 Erythromycin_replicate_3 27645242 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80251/GSE80251.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli strain NM580 (genotype MG1655 ermBL-ermB’::LacZ) cells were grown in LB broth (37°C) until OD600 of ~0.3. PGCGROWTHCONDITIONS SRR3379595 MCO000002564 OD600 of ~ 0.3 Optical Density (OD) True
growth_protocol OD600 of 0.3 E. coli strain NM580 ( genotype MG1655 ermBL-ermB ' : <Gtype> : LacZ </Gtype> ) cells were grown in LB broth ( <Temp> 37 °C </Temp> ) until <OD> OD600 of ~ 0.3 </OD> . GPL21726 GPL21726: Ion Torrent Proton (Escherichia coli) GSE80251 Genome-wide transcriptome analyses of E. coli treated with ribosome-targeting antibiotics GSM2122749 Clindamycin_replicate_1 27645242 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80251/GSE80251.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli strain NM580 (genotype MG1655 ermBL-ermB’::LacZ) cells were grown in LB broth (37°C) until OD600 of ~0.3. PGCGROWTHCONDITIONS SRR3379596 MCO000002564 OD600 of ~ 0.3 Optical Density (OD) True
growth_protocol OD600 of 0.3 E. coli strain NM580 ( genotype MG1655 ermBL-ermB ' : <Gtype> : LacZ </Gtype> ) cells were grown in LB broth ( <Temp> 37 °C </Temp> ) until <OD> OD600 of ~ 0.3 </OD> . GPL21726 GPL21726: Ion Torrent Proton (Escherichia coli) GSE80251 Genome-wide transcriptome analyses of E. coli treated with ribosome-targeting antibiotics GSM2122750 Clindamycin_replicate_2 27645242 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80251/GSE80251.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli strain NM580 (genotype MG1655 ermBL-ermB’::LacZ) cells were grown in LB broth (37°C) until OD600 of ~0.3. PGCGROWTHCONDITIONS SRR3379597 MCO000002564 OD600 of ~ 0.3 Optical Density (OD) True
growth_protocol OD600 of 0.3 E. coli strain NM580 ( genotype MG1655 ermBL-ermB ' : <Gtype> : LacZ </Gtype> ) cells were grown in LB broth ( <Temp> 37 °C </Temp> ) until <OD> OD600 of ~ 0.3 </OD> . GPL21726 GPL21726: Ion Torrent Proton (Escherichia coli) GSE80251 Genome-wide transcriptome analyses of E. coli treated with ribosome-targeting antibiotics GSM2122751 Clindamycin_replicate_3 27645242 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80251/GSE80251.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli strain NM580 (genotype MG1655 ermBL-ermB’::LacZ) cells were grown in LB broth (37°C) until OD600 of ~0.3. PGCGROWTHCONDITIONS SRR3379598 MCO000002564 OD600 of ~ 0.3 Optical Density (OD) True
growth_protocol anaerobic <Air> Aerobic and anaerobic </Air> cultures were grown in 600 mL aliquots of DM25 and incubated at <Temp> 37 °C </Temp> with an orbital shaking of 150 RPM and inoculated with 1/100th volume of overnight culture . Aerobic cultures were grown for 9 h while anaerobic cultures were grown for 16 h. GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE80451 Transcriptome analysis of E. coli REL4536 grown aerobically and anaerobically GSM2127617 Aerobic 1 28103245 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80451/GSE80451.soft.gz 100 60 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic and anaerobic cultures were grown in 600 mL aliquots of DM25 and incubated at 37°C with an orbital shaking of 150 RPM and inoculated with 1/100th volume of overnight culture. Aerobic cultures were grown for 9 h while anaerobic cultures were grown for 16 h. PGCGROWTHCONDITIONS SRR3403686 MCO000011680 Aerobic and anaerobic Aeration True
growth_protocol anaerobic <Air> Aerobic and anaerobic </Air> cultures were grown in 600 mL aliquots of DM25 and incubated at <Temp> 37 °C </Temp> with an orbital shaking of 150 RPM and inoculated with 1/100th volume of overnight culture . Aerobic cultures were grown for 9 h while anaerobic cultures were grown for 16 h. GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE80451 Transcriptome analysis of E. coli REL4536 grown aerobically and anaerobically GSM2127618 Aerobic 2 28103245 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80451/GSE80451.soft.gz 100 60 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic and anaerobic cultures were grown in 600 mL aliquots of DM25 and incubated at 37°C with an orbital shaking of 150 RPM and inoculated with 1/100th volume of overnight culture. Aerobic cultures were grown for 9 h while anaerobic cultures were grown for 16 h. PGCGROWTHCONDITIONS SRR3403687 MCO000011680 Aerobic and anaerobic Aeration True
growth_protocol anaerobic <Air> Aerobic and anaerobic </Air> cultures were grown in 600 mL aliquots of DM25 and incubated at <Temp> 37 °C </Temp> with an orbital shaking of 150 RPM and inoculated with 1/100th volume of overnight culture . Aerobic cultures were grown for 9 h while anaerobic cultures were grown for 16 h. GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE80451 Transcriptome analysis of E. coli REL4536 grown aerobically and anaerobically GSM2127619 Aerobic 3 28103245 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE80451/GSE80451.soft.gz 100 60 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Aerobic and anaerobic cultures were grown in 600 mL aliquots of DM25 and incubated at 37°C with an orbital shaking of 150 RPM and inoculated with 1/100th volume of overnight culture. Aerobic cultures were grown for 9 h while anaerobic cultures were grown for 16 h. PGCGROWTHCONDITIONS SRR3403688 MCO000011680 Aerobic and anaerobic Aeration True
characteristics wildtype genotype : <Gtype> Wildtype </Gtype> GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE81584 Mfd alters global transcription patterns in undamaged Escherichia coli cells GSM2157648 MG1655_1 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE81584/GSE81584.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: Wildtype PGCGROWTHCONDITIONS SRR3584193SRR3584194SRR3584195 MCO000002470 Wildtype Genetic background True
characteristics wildtype genotype : <Gtype> Wildtype </Gtype> GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE81584 Mfd alters global transcription patterns in undamaged Escherichia coli cells GSM2157649 MG1655_2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE81584/GSE81584.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: Wildtype PGCGROWTHCONDITIONS SRR3584196SRR3584197SRR3584198 MCO000002470 Wildtype Genetic background True
characteristics wildtype genotype : <Gtype> Wildtype </Gtype> GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE81584 Mfd alters global transcription patterns in undamaged Escherichia coli cells GSM2157650 MG1655_3 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE81584/GSE81584.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: Wildtype PGCGROWTHCONDITIONS SRR3584199SRR3584200 MCO000002470 Wildtype Genetic background True
characteristics wildtype genotype : <Gtype> Wildtype </Gtype> with <Supp> vector </Supp> GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE81584 Mfd alters global transcription patterns in undamaged Escherichia coli cells GSM2157654 MG1655_vector_1 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE81584/GSE81584.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: Wildtype with vector PGCGROWTHCONDITIONS SRR3584208SRR3584209 MCO000002470 Wildtype Genetic background True
characteristics wildtype genotype : <Gtype> Wildtype </Gtype> with <Supp> vector </Supp> GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE81584 Mfd alters global transcription patterns in undamaged Escherichia coli cells GSM2157655 MG1655_vector_2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE81584/GSE81584.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: Wildtype with vector PGCGROWTHCONDITIONS SRR3584210SRR3584211 MCO000002470 Wildtype Genetic background True
characteristics 100.003 rpos level : <Supp> 100 % </Supp> GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE87856 The genome-wide transcriptional response to varying RpoS levels in Escherichia coli. GSM2341961 100% rep1 28115545 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE87856/GSE87856.soft.gz 100 60 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json rpos level: 100% PGCGROWTHCONDITIONS SRR4416202 MCO000002063 100 % Medium supplement True
characteristics 100.003 rpos level : <Supp> 100 % </Supp> GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE87856 The genome-wide transcriptional response to varying RpoS levels in Escherichia coli. GSM2341964 100% rep2 28115545 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE87856/GSE87856.soft.gz 100 60 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json rpos level: 100% PGCGROWTHCONDITIONS SRR4416205 MCO000002063 100 % Medium supplement True
characteristics 0 rpos level : <Supp> 0 % </Supp> GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE87856 The genome-wide transcriptional response to varying RpoS levels in Escherichia coli. GSM2341962 0% rep1 28115545 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE87856/GSE87856.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json rpos level: 0% PGCGROWTHCONDITIONS SRR4416203 MCO000000748 0 % Medium supplement True
characteristics 0 rpos level : <Supp> 0 % </Supp> GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE87856 The genome-wide transcriptional response to varying RpoS levels in Escherichia coli. GSM2341965 0% rep2 28115545 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE87856/GSE87856.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json rpos level: 0% PGCGROWTHCONDITIONS SRR4416206 MCO000000748 0 % Medium supplement True
characteristics 26.0 C rpos level : <Supp> 26 % </Supp> GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE87856 The genome-wide transcriptional response to varying RpoS levels in Escherichia coli. GSM2341963 26% rep1 28115545 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE87856/GSE87856.soft.gz 100 50 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt rpos level: 26% PGCGROWTHCONDITIONS SRR4416204 MCO000003196 26 % Medium supplement True
characteristics 26.0 C rpos level : <Supp> 26 % </Supp> GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE87856 The genome-wide transcriptional response to varying RpoS levels in Escherichia coli. GSM2341966 26% rep2 28115545 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE87856/GSE87856.soft.gz 100 50 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt rpos level: 26% PGCGROWTHCONDITIONS SRR4416207 MCO000003196 26 % Medium supplement True
growth_protocol nacl 0.35 m E. coli K-12 MG1655 WT and ΔompR were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with <Supp> 0.3 M of NaCl </Supp> at mid-log pahse for <Supp> 30 min </Supp> with agitation . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE88980 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GSM2356687 WT NaCl 1 28526842 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88980/GSE88980.soft.gz 84 83 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json E. coli K-12 MG1655 WT and ΔompR were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 0.3 M of NaCl at mid-log pahse for 30 min with agitation. PGCGROWTHCONDITIONS SRR4435464 MCO000002726 0.3 M of NaCl Medium supplement True
growth_protocol nacl 0.35 m E. coli K-12 MG1655 WT and ΔompR were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with <Supp> 0.3 M of NaCl </Supp> at mid-log pahse for <Supp> 30 min </Supp> with agitation . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE88980 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GSM2356688 WT NaCl 2 28526842 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88980/GSE88980.soft.gz 84 83 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json E. coli K-12 MG1655 WT and ΔompR were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 0.3 M of NaCl at mid-log pahse for 30 min with agitation. PGCGROWTHCONDITIONS SRR4435465 MCO000002726 0.3 M of NaCl Medium supplement True
growth_protocol nacl 0.35 m E. coli K-12 MG1655 WT and ΔompR were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with <Supp> 0.3 M of NaCl </Supp> at mid-log pahse for <Supp> 30 min </Supp> with agitation . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE88980 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GSM2356689 ΔompR NaCl 1 28526842 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88980/GSE88980.soft.gz 84 83 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json E. coli K-12 MG1655 WT and ΔompR were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 0.3 M of NaCl at mid-log pahse for 30 min with agitation. PGCGROWTHCONDITIONS SRR4435466 MCO000002726 0.3 M of NaCl Medium supplement True
growth_protocol nacl 0.35 m E. coli K-12 MG1655 WT and ΔompR were grown to <Phase> mid-log phase </Phase> <Air> aerobically </Air> at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> . Then cells were treated with <Supp> 0.3 M of NaCl </Supp> at mid-log pahse for <Supp> 30 min </Supp> with agitation . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE88980 Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq] GSM2356690 ΔompR NaCl 2 28526842 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88980/GSE88980.soft.gz 84 83 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json E. coli K-12 MG1655 WT and ΔompR were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 0.3 M of NaCl at mid-log pahse for 30 min with agitation. PGCGROWTHCONDITIONS SRR4435467 MCO000002726 0.3 M of NaCl Medium supplement True
characteristics arabinose induction : <Supp> 0.2 % arabinose </Supp> GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE90056 Translation efficiency is maintained during heat shock in Escherichia coli GSM2396709 ecoli_k12_pBAD_30C_m_1 29183994 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90056/GSE90056.soft.gz 100 82 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt induction: 0.2% arabinose PGCGROWTHCONDITIONS SRR5036057 MCO000000402 0.2 % arabinose Medium supplement True
characteristics arabinose induction : <Supp> 0.2 % arabinose </Supp> GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE90056 Translation efficiency is maintained during heat shock in Escherichia coli GSM2396711 ecoli_k12_pBADsigma32wt_30C_m_1 29183994 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90056/GSE90056.soft.gz 100 82 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt induction: 0.2% arabinose PGCGROWTHCONDITIONS SRR5036059 MCO000000402 0.2 % arabinose Medium supplement True
characteristics arabinose induction : <Supp> 0.2 % arabinose </Supp> GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE90056 Translation efficiency is maintained during heat shock in Escherichia coli GSM2396713 ecoli_k12_pBAD_30C_m_2 29183994 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90056/GSE90056.soft.gz 100 82 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt induction: 0.2% arabinose PGCGROWTHCONDITIONS SRR5036061 MCO000000402 0.2 % arabinose Medium supplement True
characteristics arabinose induction : <Supp> 0.2 % arabinose </Supp> GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE90056 Translation efficiency is maintained during heat shock in Escherichia coli GSM2396715 ecoli_k12_pBADsigma32wt_30C_m_2 29183994 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90056/GSE90056.soft.gz 100 82 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt induction: 0.2% arabinose PGCGROWTHCONDITIONS SRR5036063 MCO000000402 0.2 % arabinose Medium supplement True
characteristics arabinose induction : <Supp> 0.2 % arabinose </Supp> GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE90056 Translation efficiency is maintained during heat shock in Escherichia coli GSM2396717 ecoli_k12_pBAD_30C_m_3 29183994 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90056/GSE90056.soft.gz 100 82 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt induction: 0.2% arabinose PGCGROWTHCONDITIONS SRR5036065 MCO000000402 0.2 % arabinose Medium supplement True
characteristics arabinose induction : <Supp> 0.2 % arabinose </Supp> GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE90056 Translation efficiency is maintained during heat shock in Escherichia coli GSM2396719 ecoli_k12_pBADsigma32wt_30C_m_3 29183994 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90056/GSE90056.soft.gz 100 82 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt induction: 0.2% arabinose PGCGROWTHCONDITIONS SRR5036067 MCO000000402 0.2 % arabinose Medium supplement True
characteristics arabinose induction : <Supp> 0.2 % arabinose </Supp> GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE90056 Translation efficiency is maintained during heat shock in Escherichia coli GSM2396739 ecoli_k12_pBAD_30C_m_4 29183994 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90056/GSE90056.soft.gz 100 82 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt induction: 0.2% arabinose PGCGROWTHCONDITIONS SRR5036087 MCO000000402 0.2 % arabinose Medium supplement True
characteristics arabinose induction : <Supp> 0.2 % arabinose </Supp> GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE90056 Translation efficiency is maintained during heat shock in Escherichia coli GSM2396741 ecoli_k12_pBADsigma32I54N_30C_m_1 29183994 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90056/GSE90056.soft.gz 100 82 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt induction: 0.2% arabinose PGCGROWTHCONDITIONS SRR5036089 MCO000000402 0.2 % arabinose Medium supplement True
characteristics arabinose induction : <Supp> 0.2 % arabinose </Supp> GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE90056 Translation efficiency is maintained during heat shock in Escherichia coli GSM2396743 ecoli_k12_pBADsigma32I54N_30C_m_2 29183994 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE90056/GSE90056.soft.gz 100 82 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt induction: 0.2% arabinose PGCGROWTHCONDITIONS SRR5036091 MCO000000402 0.2 % arabinose Medium supplement True
growth_protocol LB agar <Med> LB </Med> ( 5 ml ) was inoculated by a frozen stock of WT or perC-mutant strain and incubated at <Temp> 37 °C </Temp> with 225 rpm shaking . Flasks containing low-glucose DMEM were inoculated 1:100 with the overnight culture and incubated at <Temp> 37 °C </Temp> with 225 rpm shaking to mid-exponential phase ( OD600 0.3-0.5 ) . GPL14548-GPL20262 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL20262: Illumina HiSeq 2000 (Escherichia coli O127:H6 str. E2348/69) GSE91001 PerC manipulates metabolism and surface antigens in enteropathogenic Escherichia coli GSM2418921 ATCACG-D1 28224117 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE91001/GSE91001.soft.gz 100 44 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt LB (5 ml) was inoculated by a frozen stock of WT or perC-mutant strain and incubated at 37°C with 225 rpm shaking. Flasks containing low-glucose DMEM were inoculated 1:100 with the overnight culture and incubated at 37°C with 225 rpm shaking to mid-exponential phase (OD600 0.3-0.5). PGCGROWTHCONDITIONS SRR5085370 MCO000011703 LB Medium True
growth_protocol LB agar <Med> LB </Med> ( 5 ml ) was inoculated by a frozen stock of WT or perC-mutant strain and incubated at <Temp> 37 °C </Temp> with 225 rpm shaking . Flasks containing low-glucose DMEM were inoculated 1:100 with the overnight culture and incubated at <Temp> 37 °C </Temp> with 225 rpm shaking to mid-exponential phase ( OD600 0.3-0.5 ) . GPL14548-GPL20262 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL20262: Illumina HiSeq 2000 (Escherichia coli O127:H6 str. E2348/69) GSE91001 PerC manipulates metabolism and surface antigens in enteropathogenic Escherichia coli GSM2418922 CGATGT-D2 28224117 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE91001/GSE91001.soft.gz 100 44 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt LB (5 ml) was inoculated by a frozen stock of WT or perC-mutant strain and incubated at 37°C with 225 rpm shaking. Flasks containing low-glucose DMEM were inoculated 1:100 with the overnight culture and incubated at 37°C with 225 rpm shaking to mid-exponential phase (OD600 0.3-0.5). PGCGROWTHCONDITIONS SRR5085371 MCO000011703 LB Medium True
growth_protocol LB agar <Med> LB </Med> ( 5 ml ) was inoculated by a frozen stock of WT or perC-mutant strain and incubated at <Temp> 37 °C </Temp> with 225 rpm shaking . Flasks containing low-glucose DMEM were inoculated 1:100 with the overnight culture and incubated at <Temp> 37 °C </Temp> with 225 rpm shaking to mid-exponential phase ( OD600 0.3-0.5 ) . GPL14548-GPL20262 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL20262: Illumina HiSeq 2000 (Escherichia coli O127:H6 str. E2348/69) GSE91001 PerC manipulates metabolism and surface antigens in enteropathogenic Escherichia coli GSM2418923 TTAGGC-D3 28224117 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE91001/GSE91001.soft.gz 100 44 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt LB (5 ml) was inoculated by a frozen stock of WT or perC-mutant strain and incubated at 37°C with 225 rpm shaking. Flasks containing low-glucose DMEM were inoculated 1:100 with the overnight culture and incubated at 37°C with 225 rpm shaking to mid-exponential phase (OD600 0.3-0.5). PGCGROWTHCONDITIONS SRR5085373 MCO000011703 LB Medium True
data_processing iptg <Supp> Supplementary </Supp> _ files _ format _ and _ <Gtype> content : The </Gtype> columns in the processed files are the following in order : <Gtype> GeneID , Length , ORF1 </Gtype> _ 1 _ mapped _ reads ( 7655775 ) , ORF1 _ 1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 7178582 ) , ORF1 _ 2 _ mapped _ reads ( 7706927 ) , ORF1 _ 2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 7070827 ) , Svi3 _ 3 _ 1 _ mapped _ reads ( 8018710 ) , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8299205 ) , Svi3 _ 3 _ 2 _ mapped _ reads ( 7313736 ) , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8344539 ) , WT1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8074159 ) , WT2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8103887 ) , ORF1 _ 1 _ coverage , ORF1 _ 1 _ <Supp> IPTG </Supp> _ coverage , ORF1 _ 2 _ coverage , ORF1 _ 2 _ <Supp> IPTG </Supp> _ coverage , Svi3 _ 3 _ 1 _ coverage , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ coverage , Svi3 _ 3 _ 2 _ coverage , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ coverage , WT1 _ <Supp> IPTG </Supp> _ coverage , WT2 _ <Supp> IPTG </Supp> _ coverage , ORF1 _ 1 _ rpkm , ORF1 _ 1 _ <Supp> IPTG </Supp> _ rpkm , ORF1 _ 2 _ rpkm , ORF1 _ 2 _ <Supp> IPTG </Supp> _ rpkm , Svi3 _ 3 _ 1 _ rpkm , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ rpkm , Svi3 _ 3 _ 2 _ rpkm , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ rpkm , WT1 _ <Supp> IPTG </Supp> _ rpkm , WT2 _ <Supp> IPTG </Supp> _ rpkm , Symbol ( gene name ) , Description , KEGG Orthology , GO Component , GO Function , and GO Process GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433290 ORF1_1_IPTG 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Supplementary_files_format_and_content: The columns in the processed files are the following in order: GeneID, Length, ORF1_1_mapped_reads(7655775), ORF1_1_IPTG_mapped_reads(7178582), ORF1_2_mapped_reads(7706927), ORF1_2_IPTG_mapped_reads(7070827), Svi3_3_1_mapped_reads(8018710), Svi3_3_1_IPTG_mapped_reads(8299205), Svi3_3_2_mapped_reads(7313736), Svi3_3_2_IPTG_mapped_reads(8344539), WT1_IPTG_mapped_reads(8074159), WT2_IPTG_mapped_reads(8103887), ORF1_1_coverage, ORF1_1_IPTG_coverage, ORF1_2_coverage, ORF1_2_IPTG_coverage, Svi3_3_1_coverage, Svi3_3_1_IPTG_coverage, Svi3_3_2_coverage, Svi3_3_2_IPTG_coverage, WT1_IPTG_coverage, WT2_IPTG_coverage, ORF1_1_rpkm, ORF1_1_IPTG_rpkm, ORF1_2_rpkm, ORF1_2_IPTG_rpkm, Svi3_3_1_rpkm, Svi3_3_1_IPTG_rpkm, Svi3_3_2_rpkm, Svi3_3_2_IPTG_rpkm, WT1_IPTG_rpkm, WT2_IPTG_rpkm, Symbol (gene name), Description, KEGG Orthology, GO Component, GO Function, and GO Process PGCGROWTHCONDITIONS SRR5121109 MCO000002105 IPTG Medium supplement True
data_processing iptg <Supp> Supplementary </Supp> _ files _ format _ and _ <Gtype> content : The </Gtype> columns in the processed files are the following in order : <Gtype> GeneID , Length , ORF1 </Gtype> _ 1 _ mapped _ reads ( 7655775 ) , ORF1 _ 1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 7178582 ) , ORF1 _ 2 _ mapped _ reads ( 7706927 ) , ORF1 _ 2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 7070827 ) , Svi3 _ 3 _ 1 _ mapped _ reads ( 8018710 ) , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8299205 ) , Svi3 _ 3 _ 2 _ mapped _ reads ( 7313736 ) , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8344539 ) , WT1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8074159 ) , WT2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8103887 ) , ORF1 _ 1 _ coverage , ORF1 _ 1 _ <Supp> IPTG </Supp> _ coverage , ORF1 _ 2 _ coverage , ORF1 _ 2 _ <Supp> IPTG </Supp> _ coverage , Svi3 _ 3 _ 1 _ coverage , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ coverage , Svi3 _ 3 _ 2 _ coverage , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ coverage , WT1 _ <Supp> IPTG </Supp> _ coverage , WT2 _ <Supp> IPTG </Supp> _ coverage , ORF1 _ 1 _ rpkm , ORF1 _ 1 _ <Supp> IPTG </Supp> _ rpkm , ORF1 _ 2 _ rpkm , ORF1 _ 2 _ <Supp> IPTG </Supp> _ rpkm , Svi3 _ 3 _ 1 _ rpkm , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ rpkm , Svi3 _ 3 _ 2 _ rpkm , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ rpkm , WT1 _ <Supp> IPTG </Supp> _ rpkm , WT2 _ <Supp> IPTG </Supp> _ rpkm , Symbol ( gene name ) , Description , KEGG Orthology , GO Component , GO Function , and GO Process GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433291 ORF1_1 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Supplementary_files_format_and_content: The columns in the processed files are the following in order: GeneID, Length, ORF1_1_mapped_reads(7655775), ORF1_1_IPTG_mapped_reads(7178582), ORF1_2_mapped_reads(7706927), ORF1_2_IPTG_mapped_reads(7070827), Svi3_3_1_mapped_reads(8018710), Svi3_3_1_IPTG_mapped_reads(8299205), Svi3_3_2_mapped_reads(7313736), Svi3_3_2_IPTG_mapped_reads(8344539), WT1_IPTG_mapped_reads(8074159), WT2_IPTG_mapped_reads(8103887), ORF1_1_coverage, ORF1_1_IPTG_coverage, ORF1_2_coverage, ORF1_2_IPTG_coverage, Svi3_3_1_coverage, Svi3_3_1_IPTG_coverage, Svi3_3_2_coverage, Svi3_3_2_IPTG_coverage, WT1_IPTG_coverage, WT2_IPTG_coverage, ORF1_1_rpkm, ORF1_1_IPTG_rpkm, ORF1_2_rpkm, ORF1_2_IPTG_rpkm, Svi3_3_1_rpkm, Svi3_3_1_IPTG_rpkm, Svi3_3_2_rpkm, Svi3_3_2_IPTG_rpkm, WT1_IPTG_rpkm, WT2_IPTG_rpkm, Symbol (gene name), Description, KEGG Orthology, GO Component, GO Function, and GO Process PGCGROWTHCONDITIONS SRR5121110 MCO000002105 IPTG Medium supplement True
data_processing iptg <Supp> Supplementary </Supp> _ files _ format _ and _ <Gtype> content : The </Gtype> columns in the processed files are the following in order : <Gtype> GeneID , Length , ORF1 </Gtype> _ 1 _ mapped _ reads ( 7655775 ) , ORF1 _ 1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 7178582 ) , ORF1 _ 2 _ mapped _ reads ( 7706927 ) , ORF1 _ 2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 7070827 ) , Svi3 _ 3 _ 1 _ mapped _ reads ( 8018710 ) , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8299205 ) , Svi3 _ 3 _ 2 _ mapped _ reads ( 7313736 ) , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8344539 ) , WT1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8074159 ) , WT2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8103887 ) , ORF1 _ 1 _ coverage , ORF1 _ 1 _ <Supp> IPTG </Supp> _ coverage , ORF1 _ 2 _ coverage , ORF1 _ 2 _ <Supp> IPTG </Supp> _ coverage , Svi3 _ 3 _ 1 _ coverage , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ coverage , Svi3 _ 3 _ 2 _ coverage , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ coverage , WT1 _ <Supp> IPTG </Supp> _ coverage , WT2 _ <Supp> IPTG </Supp> _ coverage , ORF1 _ 1 _ rpkm , ORF1 _ 1 _ <Supp> IPTG </Supp> _ rpkm , ORF1 _ 2 _ rpkm , ORF1 _ 2 _ <Supp> IPTG </Supp> _ rpkm , Svi3 _ 3 _ 1 _ rpkm , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ rpkm , Svi3 _ 3 _ 2 _ rpkm , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ rpkm , WT1 _ <Supp> IPTG </Supp> _ rpkm , WT2 _ <Supp> IPTG </Supp> _ rpkm , Symbol ( gene name ) , Description , KEGG Orthology , GO Component , GO Function , and GO Process GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433292 ORF1_2_IPTG 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Supplementary_files_format_and_content: The columns in the processed files are the following in order: GeneID, Length, ORF1_1_mapped_reads(7655775), ORF1_1_IPTG_mapped_reads(7178582), ORF1_2_mapped_reads(7706927), ORF1_2_IPTG_mapped_reads(7070827), Svi3_3_1_mapped_reads(8018710), Svi3_3_1_IPTG_mapped_reads(8299205), Svi3_3_2_mapped_reads(7313736), Svi3_3_2_IPTG_mapped_reads(8344539), WT1_IPTG_mapped_reads(8074159), WT2_IPTG_mapped_reads(8103887), ORF1_1_coverage, ORF1_1_IPTG_coverage, ORF1_2_coverage, ORF1_2_IPTG_coverage, Svi3_3_1_coverage, Svi3_3_1_IPTG_coverage, Svi3_3_2_coverage, Svi3_3_2_IPTG_coverage, WT1_IPTG_coverage, WT2_IPTG_coverage, ORF1_1_rpkm, ORF1_1_IPTG_rpkm, ORF1_2_rpkm, ORF1_2_IPTG_rpkm, Svi3_3_1_rpkm, Svi3_3_1_IPTG_rpkm, Svi3_3_2_rpkm, Svi3_3_2_IPTG_rpkm, WT1_IPTG_rpkm, WT2_IPTG_rpkm, Symbol (gene name), Description, KEGG Orthology, GO Component, GO Function, and GO Process PGCGROWTHCONDITIONS SRR5121111 MCO000002105 IPTG Medium supplement True
data_processing iptg <Supp> Supplementary </Supp> _ files _ format _ and _ <Gtype> content : The </Gtype> columns in the processed files are the following in order : <Gtype> GeneID , Length , ORF1 </Gtype> _ 1 _ mapped _ reads ( 7655775 ) , ORF1 _ 1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 7178582 ) , ORF1 _ 2 _ mapped _ reads ( 7706927 ) , ORF1 _ 2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 7070827 ) , Svi3 _ 3 _ 1 _ mapped _ reads ( 8018710 ) , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8299205 ) , Svi3 _ 3 _ 2 _ mapped _ reads ( 7313736 ) , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8344539 ) , WT1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8074159 ) , WT2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8103887 ) , ORF1 _ 1 _ coverage , ORF1 _ 1 _ <Supp> IPTG </Supp> _ coverage , ORF1 _ 2 _ coverage , ORF1 _ 2 _ <Supp> IPTG </Supp> _ coverage , Svi3 _ 3 _ 1 _ coverage , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ coverage , Svi3 _ 3 _ 2 _ coverage , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ coverage , WT1 _ <Supp> IPTG </Supp> _ coverage , WT2 _ <Supp> IPTG </Supp> _ coverage , ORF1 _ 1 _ rpkm , ORF1 _ 1 _ <Supp> IPTG </Supp> _ rpkm , ORF1 _ 2 _ rpkm , ORF1 _ 2 _ <Supp> IPTG </Supp> _ rpkm , Svi3 _ 3 _ 1 _ rpkm , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ rpkm , Svi3 _ 3 _ 2 _ rpkm , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ rpkm , WT1 _ <Supp> IPTG </Supp> _ rpkm , WT2 _ <Supp> IPTG </Supp> _ rpkm , Symbol ( gene name ) , Description , KEGG Orthology , GO Component , GO Function , and GO Process GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433293 ORF1_2 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Supplementary_files_format_and_content: The columns in the processed files are the following in order: GeneID, Length, ORF1_1_mapped_reads(7655775), ORF1_1_IPTG_mapped_reads(7178582), ORF1_2_mapped_reads(7706927), ORF1_2_IPTG_mapped_reads(7070827), Svi3_3_1_mapped_reads(8018710), Svi3_3_1_IPTG_mapped_reads(8299205), Svi3_3_2_mapped_reads(7313736), Svi3_3_2_IPTG_mapped_reads(8344539), WT1_IPTG_mapped_reads(8074159), WT2_IPTG_mapped_reads(8103887), ORF1_1_coverage, ORF1_1_IPTG_coverage, ORF1_2_coverage, ORF1_2_IPTG_coverage, Svi3_3_1_coverage, Svi3_3_1_IPTG_coverage, Svi3_3_2_coverage, Svi3_3_2_IPTG_coverage, WT1_IPTG_coverage, WT2_IPTG_coverage, ORF1_1_rpkm, ORF1_1_IPTG_rpkm, ORF1_2_rpkm, ORF1_2_IPTG_rpkm, Svi3_3_1_rpkm, Svi3_3_1_IPTG_rpkm, Svi3_3_2_rpkm, Svi3_3_2_IPTG_rpkm, WT1_IPTG_rpkm, WT2_IPTG_rpkm, Symbol (gene name), Description, KEGG Orthology, GO Component, GO Function, and GO Process PGCGROWTHCONDITIONS SRR5121112 MCO000002105 IPTG Medium supplement True
data_processing iptg <Supp> Supplementary </Supp> _ files _ format _ and _ <Gtype> content : The </Gtype> columns in the processed files are the following in order : <Gtype> GeneID , Length , ORF1 </Gtype> _ 1 _ mapped _ reads ( 7655775 ) , ORF1 _ 1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 7178582 ) , ORF1 _ 2 _ mapped _ reads ( 7706927 ) , ORF1 _ 2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 7070827 ) , Svi3 _ 3 _ 1 _ mapped _ reads ( 8018710 ) , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8299205 ) , Svi3 _ 3 _ 2 _ mapped _ reads ( 7313736 ) , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8344539 ) , WT1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8074159 ) , WT2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8103887 ) , ORF1 _ 1 _ coverage , ORF1 _ 1 _ <Supp> IPTG </Supp> _ coverage , ORF1 _ 2 _ coverage , ORF1 _ 2 _ <Supp> IPTG </Supp> _ coverage , Svi3 _ 3 _ 1 _ coverage , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ coverage , Svi3 _ 3 _ 2 _ coverage , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ coverage , WT1 _ <Supp> IPTG </Supp> _ coverage , WT2 _ <Supp> IPTG </Supp> _ coverage , ORF1 _ 1 _ rpkm , ORF1 _ 1 _ <Supp> IPTG </Supp> _ rpkm , ORF1 _ 2 _ rpkm , ORF1 _ 2 _ <Supp> IPTG </Supp> _ rpkm , Svi3 _ 3 _ 1 _ rpkm , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ rpkm , Svi3 _ 3 _ 2 _ rpkm , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ rpkm , WT1 _ <Supp> IPTG </Supp> _ rpkm , WT2 _ <Supp> IPTG </Supp> _ rpkm , Symbol ( gene name ) , Description , KEGG Orthology , GO Component , GO Function , and GO Process GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433294 Svi3_3_1_IPTG 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Supplementary_files_format_and_content: The columns in the processed files are the following in order: GeneID, Length, ORF1_1_mapped_reads(7655775), ORF1_1_IPTG_mapped_reads(7178582), ORF1_2_mapped_reads(7706927), ORF1_2_IPTG_mapped_reads(7070827), Svi3_3_1_mapped_reads(8018710), Svi3_3_1_IPTG_mapped_reads(8299205), Svi3_3_2_mapped_reads(7313736), Svi3_3_2_IPTG_mapped_reads(8344539), WT1_IPTG_mapped_reads(8074159), WT2_IPTG_mapped_reads(8103887), ORF1_1_coverage, ORF1_1_IPTG_coverage, ORF1_2_coverage, ORF1_2_IPTG_coverage, Svi3_3_1_coverage, Svi3_3_1_IPTG_coverage, Svi3_3_2_coverage, Svi3_3_2_IPTG_coverage, WT1_IPTG_coverage, WT2_IPTG_coverage, ORF1_1_rpkm, ORF1_1_IPTG_rpkm, ORF1_2_rpkm, ORF1_2_IPTG_rpkm, Svi3_3_1_rpkm, Svi3_3_1_IPTG_rpkm, Svi3_3_2_rpkm, Svi3_3_2_IPTG_rpkm, WT1_IPTG_rpkm, WT2_IPTG_rpkm, Symbol (gene name), Description, KEGG Orthology, GO Component, GO Function, and GO Process PGCGROWTHCONDITIONS SRR5121113 MCO000002105 IPTG Medium supplement True
data_processing iptg <Supp> Supplementary </Supp> _ files _ format _ and _ <Gtype> content : The </Gtype> columns in the processed files are the following in order : <Gtype> GeneID , Length , ORF1 </Gtype> _ 1 _ mapped _ reads ( 7655775 ) , ORF1 _ 1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 7178582 ) , ORF1 _ 2 _ mapped _ reads ( 7706927 ) , ORF1 _ 2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 7070827 ) , Svi3 _ 3 _ 1 _ mapped _ reads ( 8018710 ) , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8299205 ) , Svi3 _ 3 _ 2 _ mapped _ reads ( 7313736 ) , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8344539 ) , WT1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8074159 ) , WT2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8103887 ) , ORF1 _ 1 _ coverage , ORF1 _ 1 _ <Supp> IPTG </Supp> _ coverage , ORF1 _ 2 _ coverage , ORF1 _ 2 _ <Supp> IPTG </Supp> _ coverage , Svi3 _ 3 _ 1 _ coverage , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ coverage , Svi3 _ 3 _ 2 _ coverage , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ coverage , WT1 _ <Supp> IPTG </Supp> _ coverage , WT2 _ <Supp> IPTG </Supp> _ coverage , ORF1 _ 1 _ rpkm , ORF1 _ 1 _ <Supp> IPTG </Supp> _ rpkm , ORF1 _ 2 _ rpkm , ORF1 _ 2 _ <Supp> IPTG </Supp> _ rpkm , Svi3 _ 3 _ 1 _ rpkm , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ rpkm , Svi3 _ 3 _ 2 _ rpkm , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ rpkm , WT1 _ <Supp> IPTG </Supp> _ rpkm , WT2 _ <Supp> IPTG </Supp> _ rpkm , Symbol ( gene name ) , Description , KEGG Orthology , GO Component , GO Function , and GO Process GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433295 Svi3_3_1 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Supplementary_files_format_and_content: The columns in the processed files are the following in order: GeneID, Length, ORF1_1_mapped_reads(7655775), ORF1_1_IPTG_mapped_reads(7178582), ORF1_2_mapped_reads(7706927), ORF1_2_IPTG_mapped_reads(7070827), Svi3_3_1_mapped_reads(8018710), Svi3_3_1_IPTG_mapped_reads(8299205), Svi3_3_2_mapped_reads(7313736), Svi3_3_2_IPTG_mapped_reads(8344539), WT1_IPTG_mapped_reads(8074159), WT2_IPTG_mapped_reads(8103887), ORF1_1_coverage, ORF1_1_IPTG_coverage, ORF1_2_coverage, ORF1_2_IPTG_coverage, Svi3_3_1_coverage, Svi3_3_1_IPTG_coverage, Svi3_3_2_coverage, Svi3_3_2_IPTG_coverage, WT1_IPTG_coverage, WT2_IPTG_coverage, ORF1_1_rpkm, ORF1_1_IPTG_rpkm, ORF1_2_rpkm, ORF1_2_IPTG_rpkm, Svi3_3_1_rpkm, Svi3_3_1_IPTG_rpkm, Svi3_3_2_rpkm, Svi3_3_2_IPTG_rpkm, WT1_IPTG_rpkm, WT2_IPTG_rpkm, Symbol (gene name), Description, KEGG Orthology, GO Component, GO Function, and GO Process PGCGROWTHCONDITIONS SRR5121114 MCO000002105 IPTG Medium supplement True
data_processing iptg <Supp> Supplementary </Supp> _ files _ format _ and _ <Gtype> content : The </Gtype> columns in the processed files are the following in order : <Gtype> GeneID , Length , ORF1 </Gtype> _ 1 _ mapped _ reads ( 7655775 ) , ORF1 _ 1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 7178582 ) , ORF1 _ 2 _ mapped _ reads ( 7706927 ) , ORF1 _ 2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 7070827 ) , Svi3 _ 3 _ 1 _ mapped _ reads ( 8018710 ) , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8299205 ) , Svi3 _ 3 _ 2 _ mapped _ reads ( 7313736 ) , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8344539 ) , WT1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8074159 ) , WT2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8103887 ) , ORF1 _ 1 _ coverage , ORF1 _ 1 _ <Supp> IPTG </Supp> _ coverage , ORF1 _ 2 _ coverage , ORF1 _ 2 _ <Supp> IPTG </Supp> _ coverage , Svi3 _ 3 _ 1 _ coverage , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ coverage , Svi3 _ 3 _ 2 _ coverage , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ coverage , WT1 _ <Supp> IPTG </Supp> _ coverage , WT2 _ <Supp> IPTG </Supp> _ coverage , ORF1 _ 1 _ rpkm , ORF1 _ 1 _ <Supp> IPTG </Supp> _ rpkm , ORF1 _ 2 _ rpkm , ORF1 _ 2 _ <Supp> IPTG </Supp> _ rpkm , Svi3 _ 3 _ 1 _ rpkm , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ rpkm , Svi3 _ 3 _ 2 _ rpkm , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ rpkm , WT1 _ <Supp> IPTG </Supp> _ rpkm , WT2 _ <Supp> IPTG </Supp> _ rpkm , Symbol ( gene name ) , Description , KEGG Orthology , GO Component , GO Function , and GO Process GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433296 Svi3_3_2_IPTG 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Supplementary_files_format_and_content: The columns in the processed files are the following in order: GeneID, Length, ORF1_1_mapped_reads(7655775), ORF1_1_IPTG_mapped_reads(7178582), ORF1_2_mapped_reads(7706927), ORF1_2_IPTG_mapped_reads(7070827), Svi3_3_1_mapped_reads(8018710), Svi3_3_1_IPTG_mapped_reads(8299205), Svi3_3_2_mapped_reads(7313736), Svi3_3_2_IPTG_mapped_reads(8344539), WT1_IPTG_mapped_reads(8074159), WT2_IPTG_mapped_reads(8103887), ORF1_1_coverage, ORF1_1_IPTG_coverage, ORF1_2_coverage, ORF1_2_IPTG_coverage, Svi3_3_1_coverage, Svi3_3_1_IPTG_coverage, Svi3_3_2_coverage, Svi3_3_2_IPTG_coverage, WT1_IPTG_coverage, WT2_IPTG_coverage, ORF1_1_rpkm, ORF1_1_IPTG_rpkm, ORF1_2_rpkm, ORF1_2_IPTG_rpkm, Svi3_3_1_rpkm, Svi3_3_1_IPTG_rpkm, Svi3_3_2_rpkm, Svi3_3_2_IPTG_rpkm, WT1_IPTG_rpkm, WT2_IPTG_rpkm, Symbol (gene name), Description, KEGG Orthology, GO Component, GO Function, and GO Process PGCGROWTHCONDITIONS SRR5121115 MCO000002105 IPTG Medium supplement True
data_processing iptg <Supp> Supplementary </Supp> _ files _ format _ and _ <Gtype> content : The </Gtype> columns in the processed files are the following in order : <Gtype> GeneID , Length , ORF1 </Gtype> _ 1 _ mapped _ reads ( 7655775 ) , ORF1 _ 1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 7178582 ) , ORF1 _ 2 _ mapped _ reads ( 7706927 ) , ORF1 _ 2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 7070827 ) , Svi3 _ 3 _ 1 _ mapped _ reads ( 8018710 ) , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8299205 ) , Svi3 _ 3 _ 2 _ mapped _ reads ( 7313736 ) , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8344539 ) , WT1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8074159 ) , WT2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8103887 ) , ORF1 _ 1 _ coverage , ORF1 _ 1 _ <Supp> IPTG </Supp> _ coverage , ORF1 _ 2 _ coverage , ORF1 _ 2 _ <Supp> IPTG </Supp> _ coverage , Svi3 _ 3 _ 1 _ coverage , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ coverage , Svi3 _ 3 _ 2 _ coverage , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ coverage , WT1 _ <Supp> IPTG </Supp> _ coverage , WT2 _ <Supp> IPTG </Supp> _ coverage , ORF1 _ 1 _ rpkm , ORF1 _ 1 _ <Supp> IPTG </Supp> _ rpkm , ORF1 _ 2 _ rpkm , ORF1 _ 2 _ <Supp> IPTG </Supp> _ rpkm , Svi3 _ 3 _ 1 _ rpkm , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ rpkm , Svi3 _ 3 _ 2 _ rpkm , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ rpkm , WT1 _ <Supp> IPTG </Supp> _ rpkm , WT2 _ <Supp> IPTG </Supp> _ rpkm , Symbol ( gene name ) , Description , KEGG Orthology , GO Component , GO Function , and GO Process GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433297 Svi3_3_2 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Supplementary_files_format_and_content: The columns in the processed files are the following in order: GeneID, Length, ORF1_1_mapped_reads(7655775), ORF1_1_IPTG_mapped_reads(7178582), ORF1_2_mapped_reads(7706927), ORF1_2_IPTG_mapped_reads(7070827), Svi3_3_1_mapped_reads(8018710), Svi3_3_1_IPTG_mapped_reads(8299205), Svi3_3_2_mapped_reads(7313736), Svi3_3_2_IPTG_mapped_reads(8344539), WT1_IPTG_mapped_reads(8074159), WT2_IPTG_mapped_reads(8103887), ORF1_1_coverage, ORF1_1_IPTG_coverage, ORF1_2_coverage, ORF1_2_IPTG_coverage, Svi3_3_1_coverage, Svi3_3_1_IPTG_coverage, Svi3_3_2_coverage, Svi3_3_2_IPTG_coverage, WT1_IPTG_coverage, WT2_IPTG_coverage, ORF1_1_rpkm, ORF1_1_IPTG_rpkm, ORF1_2_rpkm, ORF1_2_IPTG_rpkm, Svi3_3_1_rpkm, Svi3_3_1_IPTG_rpkm, Svi3_3_2_rpkm, Svi3_3_2_IPTG_rpkm, WT1_IPTG_rpkm, WT2_IPTG_rpkm, Symbol (gene name), Description, KEGG Orthology, GO Component, GO Function, and GO Process PGCGROWTHCONDITIONS SRR5121116 MCO000002105 IPTG Medium supplement True
data_processing iptg <Supp> Supplementary </Supp> _ files _ format _ and _ <Gtype> content : The </Gtype> columns in the processed files are the following in order : <Gtype> GeneID , Length , ORF1 </Gtype> _ 1 _ mapped _ reads ( 7655775 ) , ORF1 _ 1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 7178582 ) , ORF1 _ 2 _ mapped _ reads ( 7706927 ) , ORF1 _ 2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 7070827 ) , Svi3 _ 3 _ 1 _ mapped _ reads ( 8018710 ) , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8299205 ) , Svi3 _ 3 _ 2 _ mapped _ reads ( 7313736 ) , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8344539 ) , WT1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8074159 ) , WT2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8103887 ) , ORF1 _ 1 _ coverage , ORF1 _ 1 _ <Supp> IPTG </Supp> _ coverage , ORF1 _ 2 _ coverage , ORF1 _ 2 _ <Supp> IPTG </Supp> _ coverage , Svi3 _ 3 _ 1 _ coverage , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ coverage , Svi3 _ 3 _ 2 _ coverage , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ coverage , WT1 _ <Supp> IPTG </Supp> _ coverage , WT2 _ <Supp> IPTG </Supp> _ coverage , ORF1 _ 1 _ rpkm , ORF1 _ 1 _ <Supp> IPTG </Supp> _ rpkm , ORF1 _ 2 _ rpkm , ORF1 _ 2 _ <Supp> IPTG </Supp> _ rpkm , Svi3 _ 3 _ 1 _ rpkm , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ rpkm , Svi3 _ 3 _ 2 _ rpkm , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ rpkm , WT1 _ <Supp> IPTG </Supp> _ rpkm , WT2 _ <Supp> IPTG </Supp> _ rpkm , Symbol ( gene name ) , Description , KEGG Orthology , GO Component , GO Function , and GO Process GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433298 WT1_IPTG 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Supplementary_files_format_and_content: The columns in the processed files are the following in order: GeneID, Length, ORF1_1_mapped_reads(7655775), ORF1_1_IPTG_mapped_reads(7178582), ORF1_2_mapped_reads(7706927), ORF1_2_IPTG_mapped_reads(7070827), Svi3_3_1_mapped_reads(8018710), Svi3_3_1_IPTG_mapped_reads(8299205), Svi3_3_2_mapped_reads(7313736), Svi3_3_2_IPTG_mapped_reads(8344539), WT1_IPTG_mapped_reads(8074159), WT2_IPTG_mapped_reads(8103887), ORF1_1_coverage, ORF1_1_IPTG_coverage, ORF1_2_coverage, ORF1_2_IPTG_coverage, Svi3_3_1_coverage, Svi3_3_1_IPTG_coverage, Svi3_3_2_coverage, Svi3_3_2_IPTG_coverage, WT1_IPTG_coverage, WT2_IPTG_coverage, ORF1_1_rpkm, ORF1_1_IPTG_rpkm, ORF1_2_rpkm, ORF1_2_IPTG_rpkm, Svi3_3_1_rpkm, Svi3_3_1_IPTG_rpkm, Svi3_3_2_rpkm, Svi3_3_2_IPTG_rpkm, WT1_IPTG_rpkm, WT2_IPTG_rpkm, Symbol (gene name), Description, KEGG Orthology, GO Component, GO Function, and GO Process PGCGROWTHCONDITIONS SRR5121117 MCO000002105 IPTG Medium supplement True
data_processing iptg <Supp> Supplementary </Supp> _ files _ format _ and _ <Gtype> content : The </Gtype> columns in the processed files are the following in order : <Gtype> GeneID , Length , ORF1 </Gtype> _ 1 _ mapped _ reads ( 7655775 ) , ORF1 _ 1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 7178582 ) , ORF1 _ 2 _ mapped _ reads ( 7706927 ) , ORF1 _ 2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 7070827 ) , Svi3 _ 3 _ 1 _ mapped _ reads ( 8018710 ) , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8299205 ) , Svi3 _ 3 _ 2 _ mapped _ reads ( 7313736 ) , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8344539 ) , WT1 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8074159 ) , WT2 _ <Supp> IPTG </Supp> _ mapped _ reads ( 8103887 ) , ORF1 _ 1 _ coverage , ORF1 _ 1 _ <Supp> IPTG </Supp> _ coverage , ORF1 _ 2 _ coverage , ORF1 _ 2 _ <Supp> IPTG </Supp> _ coverage , Svi3 _ 3 _ 1 _ coverage , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ coverage , Svi3 _ 3 _ 2 _ coverage , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ coverage , WT1 _ <Supp> IPTG </Supp> _ coverage , WT2 _ <Supp> IPTG </Supp> _ coverage , ORF1 _ 1 _ rpkm , ORF1 _ 1 _ <Supp> IPTG </Supp> _ rpkm , ORF1 _ 2 _ rpkm , ORF1 _ 2 _ <Supp> IPTG </Supp> _ rpkm , Svi3 _ 3 _ 1 _ rpkm , Svi3 _ 3 _ 1 _ <Supp> IPTG </Supp> _ rpkm , Svi3 _ 3 _ 2 _ rpkm , Svi3 _ 3 _ 2 _ <Supp> IPTG </Supp> _ rpkm , WT1 _ <Supp> IPTG </Supp> _ rpkm , WT2 _ <Supp> IPTG </Supp> _ rpkm , Symbol ( gene name ) , Description , KEGG Orthology , GO Component , GO Function , and GO Process GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433299 WT2_IPTG 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Supplementary_files_format_and_content: The columns in the processed files are the following in order: GeneID, Length, ORF1_1_mapped_reads(7655775), ORF1_1_IPTG_mapped_reads(7178582), ORF1_2_mapped_reads(7706927), ORF1_2_IPTG_mapped_reads(7070827), Svi3_3_1_mapped_reads(8018710), Svi3_3_1_IPTG_mapped_reads(8299205), Svi3_3_2_mapped_reads(7313736), Svi3_3_2_IPTG_mapped_reads(8344539), WT1_IPTG_mapped_reads(8074159), WT2_IPTG_mapped_reads(8103887), ORF1_1_coverage, ORF1_1_IPTG_coverage, ORF1_2_coverage, ORF1_2_IPTG_coverage, Svi3_3_1_coverage, Svi3_3_1_IPTG_coverage, Svi3_3_2_coverage, Svi3_3_2_IPTG_coverage, WT1_IPTG_coverage, WT2_IPTG_coverage, ORF1_1_rpkm, ORF1_1_IPTG_rpkm, ORF1_2_rpkm, ORF1_2_IPTG_rpkm, Svi3_3_1_rpkm, Svi3_3_1_IPTG_rpkm, Svi3_3_2_rpkm, Svi3_3_2_IPTG_rpkm, WT1_IPTG_rpkm, WT2_IPTG_rpkm, Symbol (gene name), Description, KEGG Orthology, GO Component, GO Function, and GO Process PGCGROWTHCONDITIONS SRR5121118 MCO000002105 IPTG Medium supplement True
characteristics plasmid PK8263 (4 µM IPTG-induced fnr) induction : <Supp> induced 50 µM IPTG </Supp> GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433290 ORF1_1_IPTG 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 90 58 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt induction: induced 50 µM IPTG PGCGROWTHCONDITIONS SRR5121109 MCO000003316 induced 50 µM IPTG Medium supplement True
characteristics plasmid PK8263 (4 µM IPTG-induced fnr) induction : <Supp> induced 50 µM IPTG </Supp> GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433292 ORF1_2_IPTG 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 90 58 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt induction: induced 50 µM IPTG PGCGROWTHCONDITIONS SRR5121111 MCO000003316 induced 50 µM IPTG Medium supplement True
characteristics plasmid PK8263 (4 µM IPTG-induced fnr) induction : <Supp> induced 50 µM IPTG </Supp> GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433294 Svi3_3_1_IPTG 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 90 58 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt induction: induced 50 µM IPTG PGCGROWTHCONDITIONS SRR5121113 MCO000003316 induced 50 µM IPTG Medium supplement True
characteristics plasmid PK8263 (4 µM IPTG-induced fnr) induction : <Supp> induced 50 µM IPTG </Supp> GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433296 Svi3_3_2_IPTG 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 90 58 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt induction: induced 50 µM IPTG PGCGROWTHCONDITIONS SRR5121115 MCO000003316 induced 50 µM IPTG Medium supplement True
characteristics plasmid PK8263 (4 µM IPTG-induced fnr) induction : <Supp> induced 50 µM IPTG </Supp> GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433298 WT1_IPTG 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 90 58 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt induction: induced 50 µM IPTG PGCGROWTHCONDITIONS SRR5121117 MCO000003316 induced 50 µM IPTG Medium supplement True
characteristics plasmid PK8263 (4 µM IPTG-induced fnr) induction : <Supp> induced 50 µM IPTG </Supp> GPL18956 GPL18956: Illumina HiSeq 2500 (Escherichia coli str. K-12 substr. MG1655) GSE92601 A bacteriophage enzyme induces bacterial metabolic perturbation that confers a novel promiscuous function. GSM2433299 WT2_IPTG 29807996 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE92601/GSE92601.soft.gz 90 58 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt induction: induced 50 µM IPTG PGCGROWTHCONDITIONS SRR5121118 MCO000003316 induced 50 µM IPTG Medium supplement True
characteristics 30.0 C time point ( minutes ) : <Supp> 30 </Supp> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE93125 Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton GSM2445155 Eco_TolC_30min_control_A 28959742 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE93125/GSE93125.soft.gz 100 50 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt time point (minutes): 30 PGCGROWTHCONDITIONS SRR5143868 MCO000002695 30 Medium supplement True
characteristics 30.0 C time point ( minutes ) : <Supp> 30 </Supp> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE93125 Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton GSM2445157 Eco_TolC_30min_control_B 28959742 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE93125/GSE93125.soft.gz 100 50 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt time point (minutes): 30 PGCGROWTHCONDITIONS SRR5143869 MCO000002695 30 Medium supplement True
characteristics 30.0 C time point ( minutes ) : <Supp> 30 </Supp> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE93125 Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton GSM2445159 Eco_TolC_30min_Carolacton_A 28959742 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE93125/GSE93125.soft.gz 100 50 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt time point (minutes): 30 PGCGROWTHCONDITIONS SRR5143870 MCO000002695 30 Medium supplement True
characteristics 30.0 C time point ( minutes ) : <Supp> 30 </Supp> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE93125 Transcriptomal changes of Escherichia coli TolC grown with the biofilm inhibitor Carolacton GSM2445161 Eco_TolC_30min_Carolacton_B 28959742 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE93125/GSE93125.soft.gz 100 50 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt time point (minutes): 30 PGCGROWTHCONDITIONS SRR5143871 MCO000002695 30 Medium supplement True
extract_protocol rifampicin Single colonies of P. putida strain DOT-T1E and E. coli MG1655 were grown overnight in Luria -- <Med> Bertani ( LB ) medium </Med> at <Temp> 30 °C </Temp> . Overnight cultures were diluted to a starting OD600 of 0.01 in the same medium and 50 ml aliquots were disposed in separate 250 ml Erlenmeyer flasks and incubated with shaking at 200 rpm . When cultures reached exponential phase ( 0.5 at OD600 ) , antibiotics were added at sub-lethal concentrations to the culture medium to reach a final concentration of 1 µg/ml kanamycin , 300 µg/ml ampicillin , 150 µg/ml chloramphenicol , 4 µg/ml tetracycline , 0.5 µg/ml ciprofloxacin , 300 µg/ml spectinomycin , 500 µg/ml <Supp> rifampicin and </Supp> 2 µg/ml gentamicin . Then cultures were incubated under the same conditions for one hour more . Cells were harvested by centrifugation at 8000 g for 10 min and suspended immediately in stop solution ( 95 % ( v/v ) ethanol , 5 % ( v/v ) phenol ) and pelleted by centrifugation . After that , total RNA was extracted with Trizol ( Invitrogen ) . Removal of DNA was carried out by treatment with DNase I ( Fermentas ) in combination with the RNase inhibitor RiboLock ( Fermentas ) . The integrity and quality of total RNA was assessed with an Agilent 2100 Bioanalyzer ( Agilent Technologies ) . GPL14548-GPL19659-GPL23101 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL19659: Illumina HiSeq 2000 (Pseudomonas putida). GPL23101: Illumina HiSeq 2000 (Escherichia coli; Pseudomonas putida) GSE95310 Cross-talk between species that do not share often the same environment GSM2501621 MG1655_LB1 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95310/GSE95310.soft.gz 100 83 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Single colonies of P. putida strain DOT-T1E and E. coli MG1655 were grown overnight in Luria–Bertani (LB) medium at 30°C. Overnight cultures were diluted to a starting OD600 of 0.01 in the same medium and 50 ml aliquots were disposed in separate 250 ml Erlenmeyer flasks and incubated with shaking at 200 rpm. When cultures reached exponential phase (0.5 at OD600), antibiotics were added at sub-lethal concentrations to the culture medium to reach a final concentration of 1 µg/ml kanamycin, 300 µg/ml ampicillin, 150 µg/ml chloramphenicol, 4 µg/ml tetracycline, 0.5 µg/ml ciprofloxacin, 300 µg/ml spectinomycin, 500 µg/ml rifampicin and 2 µg/ml gentamicin. Then cultures were incubated under the same conditions for one hour more. Cells were harvested by centrifugation at 8000 g for 10 min and suspended immediately in stop solution (95% (v/v) ethanol, 5% (v/v) phenol) and pelleted by centrifugation. After that, total RNA was extracted with Trizol (Invitrogen). Removal of DNA was carried out by treatment with DNase I (Fermentas) in combination with the RNase inhibitor RiboLock (Fermentas). The integrity and quality of total RNA was assessed with an Agilent 2100 Bioanalyzer (Agilent Technologies). PGCGROWTHCONDITIONS SRR5282168 MCO000000828 rifampicin and Medium supplement True
extract_protocol rifampicin Single colonies of P. putida strain DOT-T1E and E. coli MG1655 were grown overnight in Luria -- <Med> Bertani ( LB ) medium </Med> at <Temp> 30 °C </Temp> . Overnight cultures were diluted to a starting OD600 of 0.01 in the same medium and 50 ml aliquots were disposed in separate 250 ml Erlenmeyer flasks and incubated with shaking at 200 rpm . When cultures reached exponential phase ( 0.5 at OD600 ) , antibiotics were added at sub-lethal concentrations to the culture medium to reach a final concentration of 1 µg/ml kanamycin , 300 µg/ml ampicillin , 150 µg/ml chloramphenicol , 4 µg/ml tetracycline , 0.5 µg/ml ciprofloxacin , 300 µg/ml spectinomycin , 500 µg/ml <Supp> rifampicin and </Supp> 2 µg/ml gentamicin . Then cultures were incubated under the same conditions for one hour more . Cells were harvested by centrifugation at 8000 g for 10 min and suspended immediately in stop solution ( 95 % ( v/v ) ethanol , 5 % ( v/v ) phenol ) and pelleted by centrifugation . After that , total RNA was extracted with Trizol ( Invitrogen ) . Removal of DNA was carried out by treatment with DNase I ( Fermentas ) in combination with the RNase inhibitor RiboLock ( Fermentas ) . The integrity and quality of total RNA was assessed with an Agilent 2100 Bioanalyzer ( Agilent Technologies ) . GPL14548-GPL19659-GPL23101 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL19659: Illumina HiSeq 2000 (Pseudomonas putida). GPL23101: Illumina HiSeq 2000 (Escherichia coli; Pseudomonas putida) GSE95310 Cross-talk between species that do not share often the same environment GSM2501622 MG1655_LB2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95310/GSE95310.soft.gz 100 83 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Single colonies of P. putida strain DOT-T1E and E. coli MG1655 were grown overnight in Luria–Bertani (LB) medium at 30°C. Overnight cultures were diluted to a starting OD600 of 0.01 in the same medium and 50 ml aliquots were disposed in separate 250 ml Erlenmeyer flasks and incubated with shaking at 200 rpm. When cultures reached exponential phase (0.5 at OD600), antibiotics were added at sub-lethal concentrations to the culture medium to reach a final concentration of 1 µg/ml kanamycin, 300 µg/ml ampicillin, 150 µg/ml chloramphenicol, 4 µg/ml tetracycline, 0.5 µg/ml ciprofloxacin, 300 µg/ml spectinomycin, 500 µg/ml rifampicin and 2 µg/ml gentamicin. Then cultures were incubated under the same conditions for one hour more. Cells were harvested by centrifugation at 8000 g for 10 min and suspended immediately in stop solution (95% (v/v) ethanol, 5% (v/v) phenol) and pelleted by centrifugation. After that, total RNA was extracted with Trizol (Invitrogen). Removal of DNA was carried out by treatment with DNase I (Fermentas) in combination with the RNase inhibitor RiboLock (Fermentas). The integrity and quality of total RNA was assessed with an Agilent 2100 Bioanalyzer (Agilent Technologies). PGCGROWTHCONDITIONS SRR5282169 MCO000000828 rifampicin and Medium supplement True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516609 1A_MG_t0 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304286 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516610 2A_MG_t10 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304287 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516611 3A_MG_t30 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304288 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516612 4A_MG_t60 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304289 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516613 5A_MG+Hg_t10 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304290 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516614 6A_MG+Hg_t30 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304291 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516615 7A_MG+Hg_t60 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304292 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516616 8A_MG+PMA_t10 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304293 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516617 9A_MG+PMA_t30 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304294 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516618 10A_MG+PMA_t60 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304295 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516619 1B_MG_t0 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304296 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516620 2B_MG_t10 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304297 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516621 3B_MG_t30 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304298 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516622 4B_MG_t60 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304299 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516623 5B_MG+Hg_t10 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304300 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516624 6B_MG+Hg_t30 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304301 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516625 7B_MG+Hg_t60 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304302 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516626 8B_MG+PMA_t10 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304303 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516627 9B_MG+PMA_t30 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304304 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516628 10B_MG+PMA_t60 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304305 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516629 1C_MG_t0 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304306 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516630 2C_MG_t10 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304307 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516631 3C_MG_t30 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304308 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516632 4C_MG_t60 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304309 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516633 5C_MG+Hg_t10 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304310 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516634 6C_MG+Hg_t30 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304311 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516635 7C_MG+Hg_t60 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304312 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516636 8C_MG+PMA_t10 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304313 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516637 9C_MG+PMA_t30 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304314 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol MOPS minimal medium For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani ( LB ) agar overnight at <Temp> 37 °C </Temp> . A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in <Med> Neidhardt MOPS Minimal Medium ( NM3 ) </Med> ( Neidhardt et al. , 1974 , J Bacteriol ) ( 0.2 % final glucose concentration ) supplemented with <Supp> 20 mg/L uracil </Supp> and 500 µg/L thiamine , which was incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> overnight ( ~ 18 hr ) . The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each , which were incubated at <Temp> 37 °C </Temp> with shaking at <Agit> 250 rpm </Agit> . GPL15010 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655) GSE95575 Transcriptional responses of Escherichia coli during recovery from inorganic or organic mercury exposure GSM2516638 10C_MG+PMA_t60 29338696 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95575/GSE95575.soft.gz 100 73 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For each biological RNA-Seq replicate E coli K-12 MG1655 was subcultured from cyrostorage on Luria-Bertani (LB) agar overnight at 37°C. A half-dozen well-isolated colonies were used to inoculate a 20 ml starter culture in Neidhardt MOPS Minimal Medium (NM3) (Neidhardt et al., 1974, J Bacteriol) (0.2% final glucose concentration) supplemented with 20 mg/L uracil and 500 µg/L thiamine, which was incubated at 37°C with shaking at 250 rpm overnight (~18 hr). The overnight starter culture was diluted 1:30 to initiate the experimental culture and divided into three 500 ml flasks with 100 ml NM3 in each, which were incubated at 37°C with shaking at 250 rpm. PGCGROWTHCONDITIONS SRR5304315 MCO000002528 Neidhardt MOPS Minimal Medium ( NM3 ) Medium True
growth_protocol glucose 120 mM Cells were grown in TB7 supplemented with <Supp> 22 mM glucose </Supp> until OD600 ~ 1.8 . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2563999 AR1-/AR2- rep 2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 89 89 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were grown in TB7 supplemented with 22 mM glucose until OD600 ~1.8. PGCGROWTHCONDITIONS SRR5416991 MCO000002775 22 mM glucose Medium supplement True
characteristics glucose 120 mM media : <Gtype> Tryptone </Gtype> broth buffered to pH 7 supplemented with <Supp> 22 mM glucose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2563999 AR1-/AR2- rep 2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 89 89 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: Tryptone broth buffered to pH 7 supplemented with 22 mM glucose PGCGROWTHCONDITIONS SRR5416991 MCO000002775 22 mM glucose Medium supplement True
growth_protocol glucose 120 mM Cells were grown in TB7 supplemented with <Supp> 22 mM glucose </Supp> until OD600 ~ 1.8 . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2564000 AR1-/AR2- rep 3 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 89 89 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were grown in TB7 supplemented with 22 mM glucose until OD600 ~1.8. PGCGROWTHCONDITIONS SRR5416992 MCO000002775 22 mM glucose Medium supplement True
characteristics glucose 120 mM media : <Gtype> Tryptone </Gtype> broth buffered to pH 7 supplemented with <Supp> 22 mM glucose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2564000 AR1-/AR2- rep 3 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 89 89 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: Tryptone broth buffered to pH 7 supplemented with 22 mM glucose PGCGROWTHCONDITIONS SRR5416992 MCO000002775 22 mM glucose Medium supplement True
growth_protocol glucose 120 mM Cells were grown in TB7 supplemented with <Supp> 22 mM glucose </Supp> until OD600 ~ 1.8 . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2564001 WT rep 1 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 89 89 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were grown in TB7 supplemented with 22 mM glucose until OD600 ~1.8. PGCGROWTHCONDITIONS SRR5416993 MCO000002775 22 mM glucose Medium supplement True
characteristics glucose 120 mM media : <Gtype> Tryptone </Gtype> broth buffered to pH 7 supplemented with <Supp> 22 mM glucose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2564001 WT rep 1 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 89 89 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: Tryptone broth buffered to pH 7 supplemented with 22 mM glucose PGCGROWTHCONDITIONS SRR5416993 MCO000002775 22 mM glucose Medium supplement True
growth_protocol glucose 120 mM Cells were grown in TB7 supplemented with <Supp> 22 mM glucose </Supp> until OD600 ~ 1.8 . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2564002 WT rep 2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 89 89 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were grown in TB7 supplemented with 22 mM glucose until OD600 ~1.8. PGCGROWTHCONDITIONS SRR5416994 MCO000002775 22 mM glucose Medium supplement True
characteristics glucose 120 mM media : <Gtype> Tryptone </Gtype> broth buffered to pH 7 supplemented with <Supp> 22 mM glucose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2564002 WT rep 2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 89 89 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: Tryptone broth buffered to pH 7 supplemented with 22 mM glucose PGCGROWTHCONDITIONS SRR5416994 MCO000002775 22 mM glucose Medium supplement True
growth_protocol glucose 120 mM Cells were grown in TB7 supplemented with <Supp> 22 mM glucose </Supp> until OD600 ~ 1.8 . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2564003 WT rep 3 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 89 89 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were grown in TB7 supplemented with 22 mM glucose until OD600 ~1.8. PGCGROWTHCONDITIONS SRR5416995 MCO000002775 22 mM glucose Medium supplement True
characteristics glucose 120 mM media : <Gtype> Tryptone </Gtype> broth buffered to pH 7 supplemented with <Supp> 22 mM glucose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2564003 WT rep 3 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 89 89 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: Tryptone broth buffered to pH 7 supplemented with 22 mM glucose PGCGROWTHCONDITIONS SRR5416995 MCO000002775 22 mM glucose Medium supplement True
growth_protocol glucose 120 mM Cells were grown in TB7 supplemented with <Supp> 22 mM glucose </Supp> until OD600 ~ 1.8 . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2564004 K100Q rep 1 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 89 89 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were grown in TB7 supplemented with 22 mM glucose until OD600 ~1.8. PGCGROWTHCONDITIONS SRR5416996 MCO000002775 22 mM glucose Medium supplement True
characteristics glucose 120 mM media : <Gtype> Tryptone </Gtype> broth buffered to pH 7 supplemented with <Supp> 22 mM glucose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2564004 K100Q rep 1 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 89 89 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: Tryptone broth buffered to pH 7 supplemented with 22 mM glucose PGCGROWTHCONDITIONS SRR5416996 MCO000002775 22 mM glucose Medium supplement True
growth_protocol glucose 120 mM Cells were grown in TB7 supplemented with <Supp> 22 mM glucose </Supp> until OD600 ~ 1.8 . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2564005 K100Q rep 2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 89 89 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were grown in TB7 supplemented with 22 mM glucose until OD600 ~1.8. PGCGROWTHCONDITIONS SRR5416997 MCO000002775 22 mM glucose Medium supplement True
characteristics glucose 120 mM media : <Gtype> Tryptone </Gtype> broth buffered to pH 7 supplemented with <Supp> 22 mM glucose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2564005 K100Q rep 2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 89 89 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: Tryptone broth buffered to pH 7 supplemented with 22 mM glucose PGCGROWTHCONDITIONS SRR5416997 MCO000002775 22 mM glucose Medium supplement True
growth_protocol glucose 120 mM Cells were grown in TB7 supplemented with <Supp> 22 mM glucose </Supp> until OD600 ~ 1.8 . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2564006 K100Q rep 3 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 89 89 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were grown in TB7 supplemented with 22 mM glucose until OD600 ~1.8. PGCGROWTHCONDITIONS SRR5416998 MCO000002775 22 mM glucose Medium supplement True
characteristics glucose 120 mM media : <Gtype> Tryptone </Gtype> broth buffered to pH 7 supplemented with <Supp> 22 mM glucose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2564006 K100Q rep 3 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 89 89 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: Tryptone broth buffered to pH 7 supplemented with 22 mM glucose PGCGROWTHCONDITIONS SRR5416998 MCO000002775 22 mM glucose Medium supplement True
growth_protocol glucose 120 mM Cells were grown in TB7 supplemented with <Supp> 22 mM glucose </Supp> until OD600 ~ 1.8 . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2564007 K100R rep 1 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 89 89 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were grown in TB7 supplemented with 22 mM glucose until OD600 ~1.8. PGCGROWTHCONDITIONS SRR5416999 MCO000002775 22 mM glucose Medium supplement True
characteristics glucose 120 mM media : <Gtype> Tryptone </Gtype> broth buffered to pH 7 supplemented with <Supp> 22 mM glucose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2564007 K100R rep 1 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 89 89 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: Tryptone broth buffered to pH 7 supplemented with 22 mM glucose PGCGROWTHCONDITIONS SRR5416999 MCO000002775 22 mM glucose Medium supplement True
growth_protocol glucose 120 mM Cells were grown in TB7 supplemented with <Supp> 22 mM glucose </Supp> until OD600 ~ 1.8 . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2564008 K100R rep 2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 89 89 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were grown in TB7 supplemented with 22 mM glucose until OD600 ~1.8. PGCGROWTHCONDITIONS SRR5417000 MCO000002775 22 mM glucose Medium supplement True
characteristics glucose 120 mM media : <Gtype> Tryptone </Gtype> broth buffered to pH 7 supplemented with <Supp> 22 mM glucose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2564008 K100R rep 2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 89 89 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: Tryptone broth buffered to pH 7 supplemented with 22 mM glucose PGCGROWTHCONDITIONS SRR5417000 MCO000002775 22 mM glucose Medium supplement True
growth_protocol glucose 120 mM Cells were grown in TB7 supplemented with <Supp> 22 mM glucose </Supp> until OD600 ~ 1.8 . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2564009 K100R rep 3 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 89 89 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were grown in TB7 supplemented with 22 mM glucose until OD600 ~1.8. PGCGROWTHCONDITIONS SRR5417001 MCO000002775 22 mM glucose Medium supplement True
characteristics glucose 120 mM media : <Gtype> Tryptone </Gtype> broth buffered to pH 7 supplemented with <Supp> 22 mM glucose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE97406 Role of CRP K100 positive charge on Escherichia coli global transcriptome GSM2564009 K100R rep 3 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE97406/GSE97406.soft.gz 89 89 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: Tryptone broth buffered to pH 7 supplemented with 22 mM glucose PGCGROWTHCONDITIONS SRR5417001 MCO000002775 22 mM glucose Medium supplement True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770989 Ribosome profiling at 37°C in WT cells_1 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR6001737 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770991 Ribosome profiling 30 min after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR6001739 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770994 Ribosome profiling 4 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR6001742 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770995 Ribosome profiling 6 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR6001743 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770996 Ribosome profiling 5 min after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR6001744 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770997 Ribosome profiling 10 min after shift to 10°C in WT cells_2 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR6001745 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770998 Ribosome profiling 15 min after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR6001746 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2770999 Ribosome profiling 8 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR6001747SRR6001748 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771001 Ribosome profiling at 37°C in WT cells_2 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR6001751 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771008 mRNA-seq 4 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR6001758 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771010 mRNA-seq 8 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR6001760SRR6001761 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771014 DMS-seq 30 min after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR6001766 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771015 DMS-seq 6 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR6001769 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771016 DMS-seq 8 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR6001772 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771019 Total RNA-seq 20 min after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR6001775 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771020 Total RNA-seq 4 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR6001776 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771021 Total RNA-seq 8 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR6001777 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771025 Total RNA-seq before rifampicin treatment -- 2 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR6001781 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL10328-GPL14548-GPL15010-GPL17439 GPL10328: Illumina Genome Analyzer II (Escherichia coli). GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE103421 A stress response that monitors and regulates mRNA structure is central to cold-shock adaptation GSM2771026 Total RNA-seq after rifampicin treatment -- 4 hr after shift to 10°C in WT cells 29628307 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103421/GSE103421.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR6001782 MCO000002470 wild type Genetic background True
characteristics MCO genotype : <Gtype> wild type </Gtype> GPL24020 GPL24020: Illumina NextSeq 500 (Escherichia coli K-12) GSE103937 Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli GSM2786596 WT glucose_replicate1 29474582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103937/GSE103937.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: wild type PGCGROWTHCONDITIONS SRR6048166 MCO000002470 wild type Genetic background True
characteristics MCO genotype : <Gtype> wild type </Gtype> GPL24020 GPL24020: Illumina NextSeq 500 (Escherichia coli K-12) GSE103937 Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli GSM2786597 WT glucose_replicate2 29474582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103937/GSE103937.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: wild type PGCGROWTHCONDITIONS SRR6048167 MCO000002470 wild type Genetic background True
characteristics MCO genotype : <Gtype> wild type </Gtype> GPL24020 GPL24020: Illumina NextSeq 500 (Escherichia coli K-12) GSE103937 Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli GSM2786598 WT glucose_replicate3 29474582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103937/GSE103937.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: wild type PGCGROWTHCONDITIONS SRR6048168 MCO000002470 wild type Genetic background True
characteristics MCO genotype : <Gtype> wild type </Gtype> GPL24020 GPL24020: Illumina NextSeq 500 (Escherichia coli K-12) GSE103937 Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli GSM2786599 WT_glycerol_replicate1 29474582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103937/GSE103937.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: wild type PGCGROWTHCONDITIONS SRR6048169 MCO000002470 wild type Genetic background True
characteristics MCO genotype : <Gtype> wild type </Gtype> GPL24020 GPL24020: Illumina NextSeq 500 (Escherichia coli K-12) GSE103937 Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli GSM2786600 WT_glycerol_replicate2 29474582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103937/GSE103937.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: wild type PGCGROWTHCONDITIONS SRR6048170 MCO000002470 wild type Genetic background True
characteristics MCO genotype : <Gtype> wild type </Gtype> GPL24020 GPL24020: Illumina NextSeq 500 (Escherichia coli K-12) GSE103937 Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli GSM2786601 WT_glycerol_replicate3 29474582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103937/GSE103937.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: wild type PGCGROWTHCONDITIONS SRR6048171 MCO000002470 wild type Genetic background True
characteristics MCO genotype : <Gtype> wild type </Gtype> GPL24020 GPL24020: Illumina NextSeq 500 (Escherichia coli K-12) GSE103937 Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli GSM2786602 WT UvsW_replicate1 29474582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103937/GSE103937.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: wild type PGCGROWTHCONDITIONS SRR6048172 MCO000002470 wild type Genetic background True
characteristics MCO genotype : <Gtype> wild type </Gtype> GPL24020 GPL24020: Illumina NextSeq 500 (Escherichia coli K-12) GSE103937 Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli GSM2786603 WT UvsW_replicate2 29474582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103937/GSE103937.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: wild type PGCGROWTHCONDITIONS SRR6048173 MCO000002470 wild type Genetic background True
characteristics MCO genotype : <Gtype> wild type </Gtype> GPL24020 GPL24020: Illumina NextSeq 500 (Escherichia coli K-12) GSE103937 Genome-wide relationship between R-loop formation and antisense transcription in Escherichia coli GSM2786604 WT UvsW_replicate3 29474582 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE103937/GSE103937.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: wild type PGCGROWTHCONDITIONS SRR6048174 MCO000002470 wild type Genetic background True
growth_protocol MCO <Med> LB medium </Med> at 37ºC with 200rpm agitation . GPL24145 GPL24145: Illumina NextSeq 500 (Escherichia coli 042) GSE105133 Transcriptomic study of single mutants hns, hns2 and double mutans hhahha2 and hnshns2 from enteroaggregative E. coli 042 by RNA-seq GSM2822965 E. coli 042 WT 2957708531014240 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE105133/GSE105133.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt LB medium at 37ºC with 200rpm agitation. PGCGROWTHCONDITIONS SRR6188476 MCO000002533 LB medium Medium True
characteristics MCO genotype : <Gtype> wild type </Gtype> GPL24145 GPL24145: Illumina NextSeq 500 (Escherichia coli 042) GSE105133 Transcriptomic study of single mutants hns, hns2 and double mutans hhahha2 and hnshns2 from enteroaggregative E. coli 042 by RNA-seq GSM2822965 E. coli 042 WT 2957708531014240 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE105133/GSE105133.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: wild type PGCGROWTHCONDITIONS SRR6188476 MCO000002470 wild type Genetic background True
growth_protocol MCO <Med> LB medium </Med> at 37ºC with 200rpm agitation . GPL24145 GPL24145: Illumina NextSeq 500 (Escherichia coli 042) GSE105133 Transcriptomic study of single mutants hns, hns2 and double mutans hhahha2 and hnshns2 from enteroaggregative E. coli 042 by RNA-seq GSM2822966 E. coli hhahha2 2957708531014240 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE105133/GSE105133.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt LB medium at 37ºC with 200rpm agitation. PGCGROWTHCONDITIONS SRR6188477 MCO000002533 LB medium Medium True
growth_protocol MCO <Med> LB medium </Med> at 37ºC with 200rpm agitation . GPL24145 GPL24145: Illumina NextSeq 500 (Escherichia coli 042) GSE105133 Transcriptomic study of single mutants hns, hns2 and double mutans hhahha2 and hnshns2 from enteroaggregative E. coli 042 by RNA-seq GSM2822967 E. coli hns 2957708531014240 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE105133/GSE105133.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt LB medium at 37ºC with 200rpm agitation. PGCGROWTHCONDITIONS SRR6188478 MCO000002533 LB medium Medium True
growth_protocol MCO <Med> LB medium </Med> at 37ºC with 200rpm agitation . GPL24145 GPL24145: Illumina NextSeq 500 (Escherichia coli 042) GSE105133 Transcriptomic study of single mutants hns, hns2 and double mutans hhahha2 and hnshns2 from enteroaggregative E. coli 042 by RNA-seq GSM2822968 E. coli hns2 2957708531014240 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE105133/GSE105133.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt LB medium at 37ºC with 200rpm agitation. PGCGROWTHCONDITIONS SRR6188479 MCO000002533 LB medium Medium True
growth_protocol MCO <Med> LB medium </Med> at 37ºC with 200rpm agitation . GPL24145 GPL24145: Illumina NextSeq 500 (Escherichia coli 042) GSE105133 Transcriptomic study of single mutants hns, hns2 and double mutans hhahha2 and hnshns2 from enteroaggregative E. coli 042 by RNA-seq GSM2822969 E. coli hnshns2 2957708531014240 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE105133/GSE105133.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt LB medium at 37ºC with 200rpm agitation. PGCGROWTHCONDITIONS SRR6188480 MCO000002533 LB medium Medium True
characteristics MCO genotype : <Gtype> wild type </Gtype> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3022135 WT rep1 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: wild type PGCGROWTHCONDITIONS SRR6781997 MCO000002470 wild type Genetic background True
characteristics MCO genotype : <Gtype> wild type </Gtype> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3022136 WT rep2 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: wild type PGCGROWTHCONDITIONS SRR6781998 MCO000002470 wild type Genetic background True
characteristics MCO genotype : <Gtype> wild type </Gtype> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3108934 WT-low-ph rep1 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: wild type PGCGROWTHCONDITIONS SRR7057985 MCO000002470 wild type Genetic background True
characteristics MCO genotype : <Gtype> wild type </Gtype> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3108935 WT-low-ph rep2 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: wild type PGCGROWTHCONDITIONS SRR7057986 MCO000002470 wild type Genetic background True
characteristics MCO genotype : <Gtype> wild type </Gtype> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3108936 WT-high-ph rep1 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: wild type PGCGROWTHCONDITIONS SRR7057987 MCO000002470 wild type Genetic background True
characteristics MCO genotype : <Gtype> wild type </Gtype> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE111094 Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655 (RNA-seq data set) GSM3108937 WT-high-ph rep2 33149261 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE111094/GSE111094.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: wild type PGCGROWTHCONDITIONS SRR7057988 MCO000002470 wild type Genetic background True
characteristics MCO strain : <Gtype> wild type </Gtype> GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566393 RNA-seq WT log phase related to total SEnd-seq WT log phase rep 4 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt strain: wild type PGCGROWTHCONDITIONS SRR8449235 MCO000002470 wild type Genetic background True
characteristics MCO strain : <Gtype> wild type </Gtype> GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566394 RNA-seq WT log phase related to total SEnd-seq WT log phase rep 5 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt strain: wild type PGCGROWTHCONDITIONS SRR8449236 MCO000002470 wild type Genetic background True
characteristics MCO strain : <Gtype> wild type </Gtype> GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566395 RNA-seq WT log phase related to total SEnd-seq WT log phase rep 6 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt strain: wild type PGCGROWTHCONDITIONS SRR8449237 MCO000002470 wild type Genetic background True
characteristics MCO strain : <Gtype> wild type </Gtype> GPL16085-GPL21222 GPL16085: Illumina MiSeq (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE117737 Full-length RNA profiling reveals pervasive bidirectional transcription terminators in bacteria GSM3566396 RNA-seq WT log phase related to total SEnd-seq WT stationary phase rep4 31308523 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE117737/GSE117737.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt strain: wild type PGCGROWTHCONDITIONS SRR8449238 MCO000002470 wild type Genetic background True
characteristics MCO media : <Air> anaerobic </Air> <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463579 no3_anaero__1 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: anaerobic M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR8173241 MCO000011680 anaerobic Aeration True
characteristics MCO media : <Air> anaerobic </Air> <Med> M9 minimal media </Med> w / <Supp> 2g/L glucose </Supp> GPL21433 GPL21433: Illumina HiSeq 4000 (Escherichia coli) GSE122295 Expression profiling to identify independent regulatory signals in Escherichia coli GSM3463580 no3_anaero__2 31797920 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE122295/GSE122295.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: anaerobic M9 minimal media w/ 2g/L glucose PGCGROWTHCONDITIONS SRR8173242 MCO000011680 anaerobic Aeration True
growth_protocol MCO For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275442 WT_LB_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907640 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275443 WT_LB_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907641 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275444 WT_EtOH_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907642 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275446 BaeR_KO_LB_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907644 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275447 BaeR_KO_ LB_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907645 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275448 BaeR_KO_EtOH_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907646 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275450 CpxR_KO_LB_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907648 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275451 CpxR_KO_LB_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907649 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275454 KdpE_KO_01-KCl_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907652 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275455 KdpE_KO_01-KCl_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907653 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275456 KdpE_KO_115-KCl_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907654 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275457 KdpE_KO_115-KCl_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907655 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275458 WT_01-KCl_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907656 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275459 WT_01-KCl_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907657 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275460 WT_115-KCl_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907658 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275461 WT_115-KCl_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907659 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275462 PhoB_KO_M9_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907660 MCO000002526 M9 minimal medium Medium True
characteristics MCO media : <Med> M9 minimal medium </Med> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275462 PhoB_KO_M9_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal medium PGCGROWTHCONDITIONS SRR10907660 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275463 PhoB_KO_M9_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907661 MCO000002526 M9 minimal medium Medium True
characteristics MCO media : <Med> M9 minimal medium </Med> GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275463 PhoB_KO_M9_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal medium PGCGROWTHCONDITIONS SRR10907661 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275464 PhoB_KO_M9-P_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907662 MCO000002526 M9 minimal medium Medium True
characteristics MCO media : <Med> M9 minimal medium </Med> without phosphate GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275464 PhoB_KO_M9-P_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal medium without phosphate PGCGROWTHCONDITIONS SRR10907662 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275465 PhoB_KO_M9-P_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907663 MCO000002526 M9 minimal medium Medium True
characteristics MCO media : <Med> M9 minimal medium </Med> without phosphate GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275465 PhoB_KO_M9-P_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal medium without phosphate PGCGROWTHCONDITIONS SRR10907663 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275466 WT_M9-P_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907664 MCO000002526 M9 minimal medium Medium True
characteristics MCO media : <Med> M9 minimal medium </Med> without phosphate GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275466 WT_M9-P_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal medium without phosphate PGCGROWTHCONDITIONS SRR10907664 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275467 WT_M9-P_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907665 MCO000002526 M9 minimal medium Medium True
characteristics MCO media : <Med> M9 minimal medium </Med> without phosphate GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275467 WT_M9-P_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt media: M9 minimal medium without phosphate PGCGROWTHCONDITIONS SRR10907665 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275469 ZraR_KO_LB_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907667 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275470 ZraR_KO_ZnCl2_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907668 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275471 ZraR_KO_ZnCl2_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907669 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275472 WT_ZnCl2_R1 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907670 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO For ethanol sensors ( BaeR and CpxR ) : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium to an OD600 = 0.5 , then ethanol was added to a final concentration of 5 % . Cells were grown for <Supp> 30 min </Supp> in the presence of ethanol before being collected for <Technique> ChIP-exo </Technique> and RNA-seq . For KdpDE : Cells were grown at <Temp> 37 °C </Temp> overnight in liquid Tris maleic <Med> acid minimal medium </Med> ( TMA ) supplemented with 115 mM KCl and 0.4 % w/v Glucose , then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose . Cells were inoculated in TMA with <Supp> 0.1 mM KCl </Supp> and 0.4 % w/v Glucose and collected at an OD600 = 0.5 for <Technique> ChIP-exo </Technique> and RNA-seq analysis . For PhoRB : cells were grown in liquid <Med> M9 minimal medium </Med> until OD600 = 0.5 . Then cells were washed three times with M9 minimal medium without phosphate ( M9-P ; without Na2HPO4 and KH2PO4 ) and incubated in M9-P for 60 min ( about the doubling time of MG1655 in <Med> M9 minimal medium </Med> ) at <Temp> 37 °C </Temp> . For ZraSR : Cells were grown at <Temp> 37 °C </Temp> in liquid LB medium containing 1 mM ZnCl2 . GPL24659 GPL24659: Illumina HiSeq 4000 (Escherichia coli str. K-12 substr. MG1655) GSE143855 Validated transcriptional regulatory roles for five two-component systems in E. coli (RNA-seq dataset) GSM4275473 WT_ZnCl2_R2 33172971 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE143855/GSE143855.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt For ethanol sensors (BaeR and CpxR): Cells were grown at 37 °C in liquid LB medium to an OD600=0.5, then ethanol was added to a final concentration of 5%. Cells were grown for 30 min in the presence of ethanol before being collected for ChIP-exo and RNA-seq. For KdpDE: Cells were grown at 37 °C overnight in liquid Tris maleic acid minimal medium (TMA) supplemented with 115 mM KCl and 0.4 % w/v Glucose, then washed twice with TMA containing 0.1 mM KCl and 0.4 % w/v Glucose. Cells were inoculated in TMA with 0.1 mM KCl and 0.4 % w/v Glucose and collected at an OD600=0.5 for ChIP-exo and RNA-seq analysis. For PhoRB: cells were grown in liquid M9 minimal medium until OD600=0.5. Then cells were washed three times with M9 minimal medium without phosphate (M9-P; without Na2HPO4 and KH2PO4) and incubated in M9-P for 60 min (about the doubling time of MG1655 in M9 minimal medium) at 37 °C. For ZraSR: Cells were grown at 37 °C in liquid LB medium containing 1 mM ZnCl2. PGCGROWTHCONDITIONS SRR10907671 MCO000002526 M9 minimal medium Medium True
characteristics MCO growth condition : <Air> anaerobic </Air> GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE43408 Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition. GSM1217969 pHerd30T-LL37 CK+ anaerobic 23856776 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE43408/GSE43408.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth condition: anaerobic PGCGROWTHCONDITIONS SRR958660 MCO000011680 anaerobic Aeration True
characteristics MCO growth condition : <Air> anaerobic </Air> GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE43408 Quantitative Different Analysis of antimicrobial peptide LL37 expressed in E.coli Top 10 under aerobic and anaerobic condition. GSM1217970 pHerd30T-LL37 induced +anaerobic 23856776 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE43408/GSE43408.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt growth condition: anaerobic PGCGROWTHCONDITIONS SRR958661 MCO000011680 anaerobic Aeration True
characteristics MCO treatment : <Phase> stationary phase </Phase> GPL17137 GPL17137: Illumina Genome Analyzer (Escherichia coli str. K-12 substr. MG1655) GSE46737 Genome-scale reconstruction of the sigma factor network in E. coli GSM1137316 E. coli stationary 1 24461193 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE46737/GSE46737.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: stationary phase PGCGROWTHCONDITIONS SRR847728 MCO000002866 stationary phase Growth phase True
characteristics MCO treatment : <Supp> glutamine </Supp> GPL17137 GPL17137: Illumina Genome Analyzer (Escherichia coli str. K-12 substr. MG1655) GSE46737 Genome-scale reconstruction of the sigma factor network in E. coli GSM1137318 E. coli glutamine 1 24461193 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE46737/GSE46737.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: glutamine PGCGROWTHCONDITIONS SRR847730 MCO000000836 glutamine Medium supplement True
characteristics MCO treatment : <Supp> glutamine </Supp> GPL17137 GPL17137: Illumina Genome Analyzer (Escherichia coli str. K-12 substr. MG1655) GSE46737 Genome-scale reconstruction of the sigma factor network in E. coli GSM1137319 E. coli glutamine 2 24461193 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE46737/GSE46737.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: glutamine PGCGROWTHCONDITIONS SRR847731 MCO000000836 glutamine Medium supplement True
characteristics MCO oxygen condition : <Air> anaerobic </Air> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174828 Mid log_wildtype_glc minimal media_anaerobic rep1 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt oxygen condition: anaerobic PGCGROWTHCONDITIONS SRR922265 MCO000011680 anaerobic Aeration True
characteristics MCO oxygen condition : <Air> anaerobic </Air> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174829 Mid log_wildtype_glc minimal media_anaerobic rep2 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt oxygen condition: anaerobic PGCGROWTHCONDITIONS SRR922266 MCO000011680 anaerobic Aeration True
characteristics MCO oxygen condition : <Air> anaerobic </Air> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174830 Mid log_wildtype_glc minimal media_anaerobic rep3 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt oxygen condition: anaerobic PGCGROWTHCONDITIONS SRR922267 MCO000011680 anaerobic Aeration True
characteristics MCO oxygen condition : <Air> anaerobic </Air> GPL16227 GPL16227: Illumina Genome Analyzer IIx (Escherichia coli K-12) GSE48324 Minimal metabolic pathway structure is consistent with associated macromolecular interactions. GSM1174836 Mid log_mntR KO_glc minimal media_anaerobic 24987116 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48324/GSE48324.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt oxygen condition: anaerobic PGCGROWTHCONDITIONS SRR922273 MCO000011680 anaerobic Aeration True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE48829 Escherichia coli RNA-Seq GSM1185375 WTA1 24214998 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48829/GSE48829.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR933989 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE48829 Escherichia coli RNA-Seq GSM1185376 WTB1 24214998 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48829/GSE48829.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR933990 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE48829 Escherichia coli RNA-Seq GSM1185377 WTC3 24214998 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE48829/GSE48829.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR933991 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL14548-GPL18183 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18183: Illumina HiSeq 2000 (Streptococcus pneumoniae) GSE54199 Transcriptome and marker frequency analysis of E. coli (control vs. Trimethoprim) and S. pneumoniae (control vs. HPUra/Kanamycin) GSM1310003 EC_Cont1_RNA 24725406 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54199/GSE54199.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR1124840 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL14548-GPL18183 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18183: Illumina HiSeq 2000 (Streptococcus pneumoniae) GSE54199 Transcriptome and marker frequency analysis of E. coli (control vs. Trimethoprim) and S. pneumoniae (control vs. HPUra/Kanamycin) GSM1310004 EC_Cont2_RNA 24725406 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54199/GSE54199.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR1124841 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL14548-GPL18183 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18183: Illumina HiSeq 2000 (Streptococcus pneumoniae) GSE54199 Transcriptome and marker frequency analysis of E. coli (control vs. Trimethoprim) and S. pneumoniae (control vs. HPUra/Kanamycin) GSM1310005 EC_Trim1_RNA 24725406 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54199/GSE54199.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR1124842 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL14548-GPL18183 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL18183: Illumina HiSeq 2000 (Streptococcus pneumoniae) GSE54199 Transcriptome and marker frequency analysis of E. coli (control vs. Trimethoprim) and S. pneumoniae (control vs. HPUra/Kanamycin) GSM1310006 EC_Trim2_RNA 24725406 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE54199/GSE54199.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR1124843 MCO000002470 wild type Genetic background True
growth_protocol MCO To harvest total RNA samples , overnight cultures of <Gtype> wild type </Gtype> MG1655 grown in LB at 37 ˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~ 0.4 for cultures grown in M63 . For samples grown to OD600 of ~ 0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution ( 95 % Ethanol , 5 % acid phenol ) . For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution . GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331409 LB 0.4 B1 TEX neg L1 GA 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt To harvest total RNA samples, overnight cultures of wild type MG1655 grown in LB at 37˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~0.4 for cultures grown in M63. For samples grown to OD600 of ~0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution (95% Ethanol, 5% acid phenol). For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution. PGCGROWTHCONDITIONS SRR1173965 MCO000002470 wild type Genetic background True
growth_protocol MCO To harvest total RNA samples , overnight cultures of <Gtype> wild type </Gtype> MG1655 grown in LB at 37 ˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~ 0.4 for cultures grown in M63 . For samples grown to OD600 of ~ 0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution ( 95 % Ethanol , 5 % acid phenol ) . For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution . GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331410 LB 0.4 B1 TEX pos L1 GA 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt To harvest total RNA samples, overnight cultures of wild type MG1655 grown in LB at 37˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~0.4 for cultures grown in M63. For samples grown to OD600 of ~0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution (95% Ethanol, 5% acid phenol). For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution. PGCGROWTHCONDITIONS SRR1173966 MCO000002470 wild type Genetic background True
growth_protocol MCO To harvest total RNA samples , overnight cultures of <Gtype> wild type </Gtype> MG1655 grown in LB at 37 ˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~ 0.4 for cultures grown in M63 . For samples grown to OD600 of ~ 0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution ( 95 % Ethanol , 5 % acid phenol ) . For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution . GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331411 LB 0.4 B2 TEX neg L1 HS1 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt To harvest total RNA samples, overnight cultures of wild type MG1655 grown in LB at 37˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~0.4 for cultures grown in M63. For samples grown to OD600 of ~0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution (95% Ethanol, 5% acid phenol). For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution. PGCGROWTHCONDITIONS SRR1173967 MCO000002470 wild type Genetic background True
growth_protocol MCO To harvest total RNA samples , overnight cultures of <Gtype> wild type </Gtype> MG1655 grown in LB at 37 ˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~ 0.4 for cultures grown in M63 . For samples grown to OD600 of ~ 0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution ( 95 % Ethanol , 5 % acid phenol ) . For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution . GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331412 LB 0.4 B2 TEX neg L1 HS2 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt To harvest total RNA samples, overnight cultures of wild type MG1655 grown in LB at 37˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~0.4 for cultures grown in M63. For samples grown to OD600 of ~0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution (95% Ethanol, 5% acid phenol). For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution. PGCGROWTHCONDITIONS SRR1173968 MCO000002470 wild type Genetic background True
growth_protocol MCO To harvest total RNA samples , overnight cultures of <Gtype> wild type </Gtype> MG1655 grown in LB at 37 ˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~ 0.4 for cultures grown in M63 . For samples grown to OD600 of ~ 0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution ( 95 % Ethanol , 5 % acid phenol ) . For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution . GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331413 LB 0.4 B2 TEX pos L1 HS1 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt To harvest total RNA samples, overnight cultures of wild type MG1655 grown in LB at 37˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~0.4 for cultures grown in M63. For samples grown to OD600 of ~0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution (95% Ethanol, 5% acid phenol). For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution. PGCGROWTHCONDITIONS SRR1173969 MCO000002470 wild type Genetic background True
growth_protocol MCO To harvest total RNA samples , overnight cultures of <Gtype> wild type </Gtype> MG1655 grown in LB at 37 ˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~ 0.4 for cultures grown in M63 . For samples grown to OD600 of ~ 0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution ( 95 % Ethanol , 5 % acid phenol ) . For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution . GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331414 LB 0.4 B2 TEX pos L1 HS2 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt To harvest total RNA samples, overnight cultures of wild type MG1655 grown in LB at 37˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~0.4 for cultures grown in M63. For samples grown to OD600 of ~0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution (95% Ethanol, 5% acid phenol). For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution. PGCGROWTHCONDITIONS SRR1173970 MCO000002470 wild type Genetic background True
growth_protocol MCO To harvest total RNA samples , overnight cultures of <Gtype> wild type </Gtype> MG1655 grown in LB at 37 ˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~ 0.4 for cultures grown in M63 . For samples grown to OD600 of ~ 0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution ( 95 % Ethanol , 5 % acid phenol ) . For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution . GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331415 LB 2.0 B1 TEX neg L1 GA 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt To harvest total RNA samples, overnight cultures of wild type MG1655 grown in LB at 37˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~0.4 for cultures grown in M63. For samples grown to OD600 of ~0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution (95% Ethanol, 5% acid phenol). For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution. PGCGROWTHCONDITIONS SRR1173971 MCO000002470 wild type Genetic background True
growth_protocol MCO To harvest total RNA samples , overnight cultures of <Gtype> wild type </Gtype> MG1655 grown in LB at 37 ˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~ 0.4 for cultures grown in M63 . For samples grown to OD600 of ~ 0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution ( 95 % Ethanol , 5 % acid phenol ) . For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution . GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331416 LB 2.0 B1 TEX neg L2 HS2 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt To harvest total RNA samples, overnight cultures of wild type MG1655 grown in LB at 37˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~0.4 for cultures grown in M63. For samples grown to OD600 of ~0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution (95% Ethanol, 5% acid phenol). For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution. PGCGROWTHCONDITIONS SRR1173972 MCO000002470 wild type Genetic background True
growth_protocol MCO To harvest total RNA samples , overnight cultures of <Gtype> wild type </Gtype> MG1655 grown in LB at 37 ˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~ 0.4 for cultures grown in M63 . For samples grown to OD600 of ~ 0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution ( 95 % Ethanol , 5 % acid phenol ) . For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution . GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331417 LB 2.0 B1 TEX pos L1 GA 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt To harvest total RNA samples, overnight cultures of wild type MG1655 grown in LB at 37˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~0.4 for cultures grown in M63. For samples grown to OD600 of ~0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution (95% Ethanol, 5% acid phenol). For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution. PGCGROWTHCONDITIONS SRR1173973 MCO000002470 wild type Genetic background True
growth_protocol MCO To harvest total RNA samples , overnight cultures of <Gtype> wild type </Gtype> MG1655 grown in LB at 37 ˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~ 0.4 for cultures grown in M63 . For samples grown to OD600 of ~ 0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution ( 95 % Ethanol , 5 % acid phenol ) . For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution . GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331418 LB 2.0 B1 TEX pos L2 HS2 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt To harvest total RNA samples, overnight cultures of wild type MG1655 grown in LB at 37˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~0.4 for cultures grown in M63. For samples grown to OD600 of ~0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution (95% Ethanol, 5% acid phenol). For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution. PGCGROWTHCONDITIONS SRR1173974 MCO000002470 wild type Genetic background True
growth_protocol MCO To harvest total RNA samples , overnight cultures of <Gtype> wild type </Gtype> MG1655 grown in LB at 37 ˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~ 0.4 for cultures grown in M63 . For samples grown to OD600 of ~ 0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution ( 95 % Ethanol , 5 % acid phenol ) . For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution . GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331419 LB 2.0 B2 TEX neg L1 HS1 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt To harvest total RNA samples, overnight cultures of wild type MG1655 grown in LB at 37˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~0.4 for cultures grown in M63. For samples grown to OD600 of ~0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution (95% Ethanol, 5% acid phenol). For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution. PGCGROWTHCONDITIONS SRR1173975 MCO000002470 wild type Genetic background True
growth_protocol MCO To harvest total RNA samples , overnight cultures of <Gtype> wild type </Gtype> MG1655 grown in LB at 37 ˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~ 0.4 for cultures grown in M63 . For samples grown to OD600 of ~ 0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution ( 95 % Ethanol , 5 % acid phenol ) . For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution . GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331420 LB 2.0 B2 TEX neg L1 HS2 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt To harvest total RNA samples, overnight cultures of wild type MG1655 grown in LB at 37˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~0.4 for cultures grown in M63. For samples grown to OD600 of ~0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution (95% Ethanol, 5% acid phenol). For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution. PGCGROWTHCONDITIONS SRR1173976 MCO000002470 wild type Genetic background True
growth_protocol MCO To harvest total RNA samples , overnight cultures of <Gtype> wild type </Gtype> MG1655 grown in LB at 37 ˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~ 0.4 for cultures grown in M63 . For samples grown to OD600 of ~ 0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution ( 95 % Ethanol , 5 % acid phenol ) . For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution . GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331421 LB 2.0 B2 TEX neg L2 HS2 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt To harvest total RNA samples, overnight cultures of wild type MG1655 grown in LB at 37˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~0.4 for cultures grown in M63. For samples grown to OD600 of ~0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution (95% Ethanol, 5% acid phenol). For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution. PGCGROWTHCONDITIONS SRR1173977 MCO000002470 wild type Genetic background True
growth_protocol MCO To harvest total RNA samples , overnight cultures of <Gtype> wild type </Gtype> MG1655 grown in LB at 37 ˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~ 0.4 for cultures grown in M63 . For samples grown to OD600 of ~ 0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution ( 95 % Ethanol , 5 % acid phenol ) . For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution . GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331422 LB 2.0 B2 TEX pos L1 HS1 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt To harvest total RNA samples, overnight cultures of wild type MG1655 grown in LB at 37˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~0.4 for cultures grown in M63. For samples grown to OD600 of ~0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution (95% Ethanol, 5% acid phenol). For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution. PGCGROWTHCONDITIONS SRR1173978 MCO000002470 wild type Genetic background True
growth_protocol MCO To harvest total RNA samples , overnight cultures of <Gtype> wild type </Gtype> MG1655 grown in LB at 37 ˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~ 0.4 for cultures grown in M63 . For samples grown to OD600 of ~ 0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution ( 95 % Ethanol , 5 % acid phenol ) . For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution . GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331423 LB 2.0 B2 TEX pos L1 HS2 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt To harvest total RNA samples, overnight cultures of wild type MG1655 grown in LB at 37˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~0.4 for cultures grown in M63. For samples grown to OD600 of ~0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution (95% Ethanol, 5% acid phenol). For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution. PGCGROWTHCONDITIONS SRR1173979 MCO000002470 wild type Genetic background True
growth_protocol MCO To harvest total RNA samples , overnight cultures of <Gtype> wild type </Gtype> MG1655 grown in LB at 37 ˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~ 0.4 for cultures grown in M63 . For samples grown to OD600 of ~ 0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution ( 95 % Ethanol , 5 % acid phenol ) . For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution . GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331424 LB 2.0 B2 TEX pos L2 HS2 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt To harvest total RNA samples, overnight cultures of wild type MG1655 grown in LB at 37˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~0.4 for cultures grown in M63. For samples grown to OD600 of ~0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution (95% Ethanol, 5% acid phenol). For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution. PGCGROWTHCONDITIONS SRR1173980 MCO000002470 wild type Genetic background True
growth_protocol MCO To harvest total RNA samples , overnight cultures of <Gtype> wild type </Gtype> MG1655 grown in LB at 37 ˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~ 0.4 for cultures grown in M63 . For samples grown to OD600 of ~ 0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution ( 95 % Ethanol , 5 % acid phenol ) . For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution . GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331426 M63 0.4 B1 TEX pos L1 GA 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt To harvest total RNA samples, overnight cultures of wild type MG1655 grown in LB at 37˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~0.4 for cultures grown in M63. For samples grown to OD600 of ~0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution (95% Ethanol, 5% acid phenol). For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution. PGCGROWTHCONDITIONS SRR1173982 MCO000002470 wild type Genetic background True
growth_protocol MCO To harvest total RNA samples , overnight cultures of <Gtype> wild type </Gtype> MG1655 grown in LB at 37 ˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~ 0.4 for cultures grown in M63 . For samples grown to OD600 of ~ 0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution ( 95 % Ethanol , 5 % acid phenol ) . For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution . GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331427 M63 0.4 B2 TEX neg L1 HS1 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt To harvest total RNA samples, overnight cultures of wild type MG1655 grown in LB at 37˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~0.4 for cultures grown in M63. For samples grown to OD600 of ~0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution (95% Ethanol, 5% acid phenol). For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution. PGCGROWTHCONDITIONS SRR1173983 MCO000002470 wild type Genetic background True
growth_protocol MCO To harvest total RNA samples , overnight cultures of <Gtype> wild type </Gtype> MG1655 grown in LB at 37 ˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~ 0.4 for cultures grown in M63 . For samples grown to OD600 of ~ 0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution ( 95 % Ethanol , 5 % acid phenol ) . For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution . GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331428 M63 0.4 B2 TEX neg L1 HS2 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt To harvest total RNA samples, overnight cultures of wild type MG1655 grown in LB at 37˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~0.4 for cultures grown in M63. For samples grown to OD600 of ~0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution (95% Ethanol, 5% acid phenol). For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution. PGCGROWTHCONDITIONS SRR1173984 MCO000002470 wild type Genetic background True
growth_protocol MCO To harvest total RNA samples , overnight cultures of <Gtype> wild type </Gtype> MG1655 grown in LB at 37 ˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~ 0.4 for cultures grown in M63 . For samples grown to OD600 of ~ 0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution ( 95 % Ethanol , 5 % acid phenol ) . For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution . GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331429 M63 0.4 B2 TEX pos L1 HS1 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt To harvest total RNA samples, overnight cultures of wild type MG1655 grown in LB at 37˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~0.4 for cultures grown in M63. For samples grown to OD600 of ~0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution (95% Ethanol, 5% acid phenol). For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution. PGCGROWTHCONDITIONS SRR1173985 MCO000002470 wild type Genetic background True
growth_protocol MCO To harvest total RNA samples , overnight cultures of <Gtype> wild type </Gtype> MG1655 grown in LB at 37 ˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~ 0.4 for cultures grown in M63 . For samples grown to OD600 of ~ 0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution ( 95 % Ethanol , 5 % acid phenol ) . For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution . GPL15010-GPL17024 GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL17024: Illumina Genome Analyzer IIx (Escherichia coli str. K-12 substr. MG1655) GSE55199 Identification and validation of antisense RNAs in Escherichia coli GSM1331430 M63 0.4 B2 TEX pos L1 HS2 25266388 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE55199/GSE55199.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt To harvest total RNA samples, overnight cultures of wild type MG1655 grown in LB at 37˚C were diluted back 1:500 in either fresh LB or M63 minimal glucose medium and allowed to grow until the cultures reached an OD600 of ~ 0.4 and 2.0 for cultures grown in LB and an OD600 of ~0.4 for cultures grown in M63. For samples grown to OD600 of ~0.4 a total volume of 25 ml of cells were harvested and combined with 5 ml of stop solution (95% Ethanol, 5% acid phenol). For samples grown to OD600 of 2.0 a total volume of 5 ml of cells were harvested and combined with 1 ml of stop solution. PGCGROWTHCONDITIONS SRR1173986 MCO000002470 wild type Genetic background True
source_name MCO <Gtype> WT </Gtype> _ <Supp> glucose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602347 WT glucose 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt WT_glucose PGCGROWTHCONDITIONS SRR1787590 MCO000000245 glucose Medium supplement True
characteristics MCO carbon source : <Supp> glucose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602347 WT glucose 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt carbon source: glucose PGCGROWTHCONDITIONS SRR1787590 MCO000000245 glucose Medium supplement True
source_name MCO <Gtype> WT </Gtype> _ <Supp> glucose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602348 WT glucose 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt WT_glucose PGCGROWTHCONDITIONS SRR1787591 MCO000000245 glucose Medium supplement True
characteristics MCO carbon source : <Supp> glucose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602348 WT glucose 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt carbon source: glucose PGCGROWTHCONDITIONS SRR1787591 MCO000000245 glucose Medium supplement True
characteristics MCO carbon source : <Supp> fructose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602349 WT fructose 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt carbon source: fructose PGCGROWTHCONDITIONS SRR1787592 MCO000000848 fructose Medium supplement True
characteristics MCO carbon source : <Supp> fructose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602350 WT fructose 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt carbon source: fructose PGCGROWTHCONDITIONS SRR1787593 MCO000000848 fructose Medium supplement True
source_name MCO <Gtype> WT </Gtype> _ <Supp> acetate </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602351 WT acetate 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt WT_acetate PGCGROWTHCONDITIONS SRR1787594 MCO000000910 acetate Medium supplement True
characteristics MCO carbon source : <Supp> acetate </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602351 WT acetate 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt carbon source: acetate PGCGROWTHCONDITIONS SRR1787594 MCO000000910 acetate Medium supplement True
source_name MCO <Gtype> WT </Gtype> _ <Supp> acetate </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602352 WT acetate 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt WT_acetate PGCGROWTHCONDITIONS SRR1787595 MCO000000910 acetate Medium supplement True
characteristics MCO carbon source : <Supp> acetate </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602352 WT acetate 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt carbon source: acetate PGCGROWTHCONDITIONS SRR1787595 MCO000000910 acetate Medium supplement True
source_name MCO <Gtype> Δcra </Gtype> _ <Supp> glucose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602353 Δcra glucose 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Δcra_glucose PGCGROWTHCONDITIONS SRR1787596 MCO000000245 glucose Medium supplement True
characteristics MCO carbon source : <Supp> glucose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602353 Δcra glucose 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt carbon source: glucose PGCGROWTHCONDITIONS SRR1787596 MCO000000245 glucose Medium supplement True
source_name MCO <Gtype> Δcra </Gtype> _ <Supp> glucose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602354 Δcra glucose 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Δcra_glucose PGCGROWTHCONDITIONS SRR1787597 MCO000000245 glucose Medium supplement True
characteristics MCO carbon source : <Supp> glucose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602354 Δcra glucose 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt carbon source: glucose PGCGROWTHCONDITIONS SRR1787597 MCO000000245 glucose Medium supplement True
characteristics MCO carbon source : <Supp> fructose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602355 Δcra fructose 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt carbon source: fructose PGCGROWTHCONDITIONS SRR1787598 MCO000000848 fructose Medium supplement True
characteristics MCO carbon source : <Supp> fructose </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602356 Δcra fructose 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt carbon source: fructose PGCGROWTHCONDITIONS SRR1787599 MCO000000848 fructose Medium supplement True
source_name MCO <Gtype> Δcra </Gtype> _ <Supp> acetate </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602357 Δcra acetate 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Δcra_acetate PGCGROWTHCONDITIONS SRR1787600 MCO000000910 acetate Medium supplement True
characteristics MCO carbon source : <Supp> acetate </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602357 Δcra acetate 1 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt carbon source: acetate PGCGROWTHCONDITIONS SRR1787600 MCO000000910 acetate Medium supplement True
source_name MCO <Gtype> Δcra </Gtype> _ <Supp> acetate </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602358 Δcra acetate 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Δcra_acetate PGCGROWTHCONDITIONS SRR1787601 MCO000000910 acetate Medium supplement True
characteristics MCO carbon source : <Supp> acetate </Supp> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE65642 Systems approach elucidates convoluted transcriptional regulation on carbon metabolism [RNA-seq] GSM1602358 Δcra acetate 2 29394395 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65642/GSE65642.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt carbon source: acetate PGCGROWTHCONDITIONS SRR1787601 MCO000000910 acetate Medium supplement True
growth_protocol MCO E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623160 WT pH5.5 1 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824557 MCO000003211 pH 5.5 pH True
growth_protocol MCO E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623161 WT pH5.5 2 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824558 MCO000003211 pH 5.5 pH True
growth_protocol MCO E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623162 ΔgadE pH5.5 1 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824559 MCO000003211 pH 5.5 pH True
growth_protocol MCO E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623163 ΔgadE pH5.5 2 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824560 MCO000003211 pH 5.5 pH True
growth_protocol MCO E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623164 ΔgadW pH5.5 1 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824561 MCO000003211 pH 5.5 pH True
growth_protocol MCO E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623165 ΔgadW pH5.5 2 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824562 MCO000003211 pH 5.5 pH True
growth_protocol MCO E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623166 ΔgadX pH5.5 1 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824563 MCO000003211 pH 5.5 pH True
growth_protocol MCO E. coli K-12 MG1655 WT , gadE , gadW and gadX mutant cells were grown to <Phase> mid-log phase </Phase> ( <OD> OD600 = 0.3 </OD> ) <Air> aerobically </Air> ( <Agit> 250 rpm </Agit> ) at <Temp> 37 °C </Temp> in <Med> M9 minimal media </Med> supplemented with <Supp> 0.2 % glucose </Supp> at <pH> pH 5.5 </pH> . GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE66481 Decoding genome-wide GadEWX transcriptional regulatory networks reveals a multifaceted cellular response to acid stress in Escherichia coli [RNA-seq] GSM1623167 ΔgadX pH5.5 2 26258987 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE66481/GSE66481.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt E. coli K-12 MG1655 WT, gadE, gadW and gadX mutant cells were grown to mid-log phase (OD600 = 0.3) aerobically (250 rpm) at 37°C in M9 minimal media supplemented with 0.2% glucose at pH 5.5. PGCGROWTHCONDITIONS SRR1824564 MCO000003211 pH 5.5 pH True
characteristics MCO genotype : <Gtype> wild type </Gtype> GPL21021 GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933) GSE73969 RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933 GSM1906887 wt_1 2717363528288207 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73969/GSE73969.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: wild type PGCGROWTHCONDITIONS SRR2637695 MCO000002470 wild type Genetic background True
characteristics MCO genotype : <Gtype> wild type </Gtype> GPL21021 GPL21021: Illumina HiSeq 2500 (Escherichia coli O157:H7 str. EDL933) GSE73969 RNA-seq to profile the H-NS transcriptome in EHEC O157:H7 strain EDL933 GSM1906888 wt_2 2717363528288207 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE73969/GSE73969.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: wild type PGCGROWTHCONDITIONS SRR2637696 MCO000002470 wild type Genetic background True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968346 WTRep1_0min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982421 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968346 WTRep1_0min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982421 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968347 WTRep1_2min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982422 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968347 WTRep1_2min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982422 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968348 WTRep1_4min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982423 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968348 WTRep1_4min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982423 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968349 WTRep1_6min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982424 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968349 WTRep1_6min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982424 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968350 WTRep1_8min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982425 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968350 WTRep1_8min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982425 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968351 WTRep1_10min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982426 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968351 WTRep1_10min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982426 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968352 WTRep1_15min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982427 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968352 WTRep1_15min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982427 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968353 WTRep1_20min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982428 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968353 WTRep1_20min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982428 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968354 WTRep2_0min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982429 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968354 WTRep2_0min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982429 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968355 WTRep2_2min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982430 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968355 WTRep2_2min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982430 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968356 WTRep2_4min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982431 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968356 WTRep2_4min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982431 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968357 WTRep2_6min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982432 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968357 WTRep2_6min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982432 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968358 WTRep2_8min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982433 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968358 WTRep2_8min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982433 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968359 WTRep2_10min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982434 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968359 WTRep2_10min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982434 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968360 WTRep2_15min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982435 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968360 WTRep2_15min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982435 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968361 WTRep2_20min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982436 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968361 WTRep2_20min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982436 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968362 WTKasRep1_0min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982437 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968362 WTKasRep1_0min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982437 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968364 WTKasRep1_4min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982439 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968364 WTKasRep1_4min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982439 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968368 WTKasRep1_15min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982443 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968368 WTKasRep1_15min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982443 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968369 WTKasRep1_20min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982444 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968369 WTKasRep1_20min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982444 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968370 WTKasRep2_0min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982445 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968370 WTKasRep2_0min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982445 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968371 WTKasRep2_2min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982446 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968371 WTKasRep2_2min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982446 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968372 WTKasRep2_4min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982447 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968372 WTKasRep2_4min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982447 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968374 WTKasRep2_8min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982449 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968374 WTKasRep2_8min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982449 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968377 WTKasRep2_20min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982452 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968377 WTKasRep2_20min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982452 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968378 MutRep1_0min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982453 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968378 MutRep1_0min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982453 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968379 MutRep1_2min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982454 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968379 MutRep1_2min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982454 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968380 MutRep1_4min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982455 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968380 MutRep1_4min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982455 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968381 MutRep1_6min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982456 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968381 MutRep1_6min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982456 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968382 MutRep1_8min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982457 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968382 MutRep1_8min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982457 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968383 MutRep1_10min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982458 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968383 MutRep1_10min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982458 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968384 MutRep1_15min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982459 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968384 MutRep1_15min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982459 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968385 MutRep1_20min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982460 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968385 MutRep1_20min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982460 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968386 MutRep2_0min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982461 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968386 MutRep2_0min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982461 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968387 MutRep2_2min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982462 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968387 MutRep2_2min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982462 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968388 MutRep2_4min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982463 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968388 MutRep2_4min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982463 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968389 MutRep2_6min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982464 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968389 MutRep2_6min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982464 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968390 MutRep2_8min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982465 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968390 MutRep2_8min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982465 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968391 MutRep2_10min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982466 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968391 MutRep2_10min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982466 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968392 MutRep2_15min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982467 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968392 MutRep2_15min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982467 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968393 MutRep2_20min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982468 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968393 MutRep2_20min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982468 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968394 neo_0min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982469 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968394 neo_0min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982469 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968395 neo_1min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982470 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968395 neo_1min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982470 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968396 neo_2min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982471 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968396 neo_2min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982471 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968397 neo_4min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982472 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968397 neo_4min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982472 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968398 neo_6min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982473 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968398 neo_6min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982473 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968399 neo_8min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982474 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968399 neo_8min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982474 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968400 neo_10min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982475 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968400 neo_10min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982475 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968401 neo_15min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982476 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968401 neo_15min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982476 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968402 bla_0min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982477 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968402 bla_0min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982477 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968403 bla_1min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982478 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968403 bla_1min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982478 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968404 bla_2min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982479 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968404 bla_2min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982479 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968405 bla_4min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982480 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968405 bla_4min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982480 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968406 bla_6min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982481 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968406 bla_6min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982481 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968407 bla_8min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982482 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968407 bla_8min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982482 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968408 bla_10min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982483 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968408 bla_10min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982483 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968409 bla_15min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982484 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968409 bla_15min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982484 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968410 mMaple3_0min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982485 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968410 mMaple3_0min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982485 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968411 mMaple3_1min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982486 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968411 mMaple3_1min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982486 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968412 mMaple3_2min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982487 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968412 mMaple3_2min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982487 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968413 mMaple3_4min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982488 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968413 mMaple3_4min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982488 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968414 mMaple3_6min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982489 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968414 mMaple3_6min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982489 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968415 mMaple3_8min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982490 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968415 mMaple3_8min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982490 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968416 mMaple3_10min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982491 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968416 mMaple3_10min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982491 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968417 mMaple3_15min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982492 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968417 mMaple3_15min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982492 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968418 phoA_0min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982493 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968418 phoA_0min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982493 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968419 phoA_1min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982494 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968419 phoA_1min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982494 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968420 phoA_2min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982495 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968420 phoA_2min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982495 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968421 phoA_4min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982496 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968421 phoA_4min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982496 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968422 phoA_6min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982497 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968422 phoA_6min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982497 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968423 phoA_8min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982498 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968423 phoA_8min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982498 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968424 phoA_10min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982499 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968424 phoA_10min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982499 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968425 phoA_15min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982500 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968425 phoA_15min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982500 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968426 lacZ_0min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982501 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968426 lacZ_0min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982501 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968427 lacZ_1min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982502 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968427 lacZ_1min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982502 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968428 lacZ_2min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982503 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968428 lacZ_2min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982503 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968429 lacZ_4min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982504 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968429 lacZ_4min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982504 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968430 lacZ_6min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982505 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968430 lacZ_6min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982505 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968431 lacZ_8min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982506 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968431 lacZ_8min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982506 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968432 lacZ_10min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982507 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968432 lacZ_10min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982507 MCO000000828 rifampicin Medium supplement True
treatment_protocol MCO Cells were treated with <Supp> rifampicin </Supp> before sample collection . In some cases , kasugamycin was added 15 minutes before the rifampicin addition . GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968433 lacZ_15min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Cells were treated with rifampicin before sample collection. In some cases, kasugamycin was added 15 minutes before the rifampicin addition. PGCGROWTHCONDITIONS SRR2982508 MCO000000828 rifampicin Medium supplement True
characteristics MCO treatment : <Supp> rifampicin </Supp> GPL14548-GPL21222 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL21222: Illumina NextSeq 500 (Escherichia coli) GSE75818 Spatial organization shapes the turnover of a bacterial transcriptome GSM1968433 lacZ_15min 27198188 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE75818/GSE75818.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt treatment: rifampicin PGCGROWTHCONDITIONS SRR2982508 MCO000000828 rifampicin Medium supplement True
characteristics MCO genotype : <Gtype> wild type </Gtype> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE76167 Mechanisms of nickel toxicity in bacteria GSM1975603 Rodrigue_1-WT-phiNi-1 2737513027668277 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE76167/GSE76167.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: wild type PGCGROWTHCONDITIONS SRR3033275SRR3033276SRR3033277SRR3033278SRR3033279 MCO000002470 wild type Genetic background True
characteristics MCO genotype : <Gtype> wild type </Gtype> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE76167 Mechanisms of nickel toxicity in bacteria GSM1975604 Rodrigue_6-WT-phiNi-2 2737513027668277 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE76167/GSE76167.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: wild type PGCGROWTHCONDITIONS SRR3033280SRR3033281SRR3033282SRR3033283 MCO000002470 wild type Genetic background True
characteristics MCO genotype : <Gtype> wild type </Gtype> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE76167 Mechanisms of nickel toxicity in bacteria GSM1975605 Rodrigue_9-WT-phiNi-3 2737513027668277 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE76167/GSE76167.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: wild type PGCGROWTHCONDITIONS SRR3033284SRR3033285SRR3033286SRR3033287 MCO000002470 wild type Genetic background True
characteristics MCO genotype : <Gtype> wild type </Gtype> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE76167 Mechanisms of nickel toxicity in bacteria GSM1975606 Rodrigue_2-WT-Ni-1 2737513027668277 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE76167/GSE76167.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: wild type PGCGROWTHCONDITIONS SRR3033288SRR3033289SRR3033290SRR3033291SRR3033292 MCO000002470 wild type Genetic background True
characteristics MCO genotype : <Gtype> wild type </Gtype> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE76167 Mechanisms of nickel toxicity in bacteria GSM1975607 Rodrigue_5-WT-Ni-2 2737513027668277 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE76167/GSE76167.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: wild type PGCGROWTHCONDITIONS SRR3033293SRR3033294SRR3033295SRR3033296 MCO000002470 wild type Genetic background True
characteristics MCO genotype : <Gtype> wild type </Gtype> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE76167 Mechanisms of nickel toxicity in bacteria GSM1975608 Rodrigue_10-WT-Ni-3 2737513027668277 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE76167/GSE76167.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype: wild type PGCGROWTHCONDITIONS SRR3033297SRR3033298SRR3033299SRR3033300SRR3033301 MCO000002470 wild type Genetic background True
growth_protocol MCO Escherichia coli strains E.coli C ( DSMZ 4860 ) , E. coli Crooks ( DSMZ 1576 ) , E. coli DH5α ( DSMZ 6897 ) E. coli W ( DSMZ 1116 ) , E. coli W3110 ( DSMZ 5911 ) were obtained from DSMZ-German Collection of Microorganism and Cell Cultures ; E. coli BL21 ( DE3 ) was purchased as competent cells from Agilent ( Agilent Technologies Inc. , USA ) , E. coli K-12 MG1655 ( ATCC 700926 ) . All strains were cultured in <Med> M9 minimal medium </Med> ( 1 ) containing Na2HPO4 x 7H2O ( 6.8 g ) , KH2PO4 ( 3 g ) , NaCl ( 0.5 g ) , NH4Cl ( 1 g ) , MgSO4 ( 2 mmol ) , CaCl2 ( 0.1 mmol ) , trace elements , Wolf 's vitamin solution ( 2 ) and glucose ( 2 g L-1 ) . Anoxic <Med> M9 minimal media </Med> with <Supp> glucose </Supp> was obtained by flushing solution with oxygen free nitrogen ( 95 % ) . Overnight cultures from single colonies of each of seven E. coli strains were diluted to a starting optical density ( OD600 ) of 0.01 . Cultures were grown in 250 ml flasks or 300 ml oxygen-free sealed bottles containing 50 ml <Med> glucose-M9 minimal media </Med> in a shaking incubator at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> . GPL14548-GPL15010-GPL21530-GPL21531-GPL21532-GPL21533-GPL21534 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL21530: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. W3110). GPL21531: Illumina HiSeq 2000 (Escherichia coli W). GPL21532: Illumina HiSeq 2000 (Escherichia coli C). GPL21533: Illumina HiSeq 2000 (Escherichia coli BL21). GPL21534: Illumina HiSeq 2000 (Escherichia coli DH5[alpha]) GSE78756 Quantifying variation within the bacterial species E. coli GSM2075722 Crooks_aero 27667363 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE78756/GSE78756.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Escherichia coli strains E.coli C (DSMZ 4860), E. coli Crooks (DSMZ 1576), E. coli DH5α (DSMZ 6897) E. coli W (DSMZ 1116), E. coli W3110 (DSMZ 5911) were obtained from DSMZ-German Collection of Microorganism and Cell Cultures; E. coli BL21 (DE3) was purchased as competent cells from Agilent (Agilent Technologies Inc., USA), E. coli K-12 MG1655 (ATCC 700926). All strains were cultured in M9 minimal medium (1) containing Na2HPO4 x 7H2O (6.8 g), KH2PO4 (3 g), NaCl (0.5 g), NH4Cl (1 g), MgSO4 (2 mmol), CaCl2 (0.1 mmol), trace elements, Wolf’s vitamin solution (2) and glucose (2 g L-1). Anoxic M9 minimal media with glucose was obtained by flushing solution with oxygen free nitrogen (95%). Overnight cultures from single colonies of each of seven E. coli strains were diluted to a starting optical density (OD600) of 0.01. Cultures were grown in 250 ml flasks or 300 ml oxygen-free sealed bottles containing 50 ml glucose-M9 minimal media in a shaking incubator at 37°C and 250 rpm. PGCGROWTHCONDITIONS SRR3194453 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO Escherichia coli strains E.coli C ( DSMZ 4860 ) , E. coli Crooks ( DSMZ 1576 ) , E. coli DH5α ( DSMZ 6897 ) E. coli W ( DSMZ 1116 ) , E. coli W3110 ( DSMZ 5911 ) were obtained from DSMZ-German Collection of Microorganism and Cell Cultures ; E. coli BL21 ( DE3 ) was purchased as competent cells from Agilent ( Agilent Technologies Inc. , USA ) , E. coli K-12 MG1655 ( ATCC 700926 ) . All strains were cultured in <Med> M9 minimal medium </Med> ( 1 ) containing Na2HPO4 x 7H2O ( 6.8 g ) , KH2PO4 ( 3 g ) , NaCl ( 0.5 g ) , NH4Cl ( 1 g ) , MgSO4 ( 2 mmol ) , CaCl2 ( 0.1 mmol ) , trace elements , Wolf 's vitamin solution ( 2 ) and glucose ( 2 g L-1 ) . Anoxic <Med> M9 minimal media </Med> with <Supp> glucose </Supp> was obtained by flushing solution with oxygen free nitrogen ( 95 % ) . Overnight cultures from single colonies of each of seven E. coli strains were diluted to a starting optical density ( OD600 ) of 0.01 . Cultures were grown in 250 ml flasks or 300 ml oxygen-free sealed bottles containing 50 ml <Med> glucose-M9 minimal media </Med> in a shaking incubator at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> . GPL14548-GPL15010-GPL21530-GPL21531-GPL21532-GPL21533-GPL21534 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL21530: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. W3110). GPL21531: Illumina HiSeq 2000 (Escherichia coli W). GPL21532: Illumina HiSeq 2000 (Escherichia coli C). GPL21533: Illumina HiSeq 2000 (Escherichia coli BL21). GPL21534: Illumina HiSeq 2000 (Escherichia coli DH5[alpha]) GSE78756 Quantifying variation within the bacterial species E. coli GSM2075722 Crooks_aero 27667363 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE78756/GSE78756.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Escherichia coli strains E.coli C (DSMZ 4860), E. coli Crooks (DSMZ 1576), E. coli DH5α (DSMZ 6897) E. coli W (DSMZ 1116), E. coli W3110 (DSMZ 5911) were obtained from DSMZ-German Collection of Microorganism and Cell Cultures; E. coli BL21 (DE3) was purchased as competent cells from Agilent (Agilent Technologies Inc., USA), E. coli K-12 MG1655 (ATCC 700926). All strains were cultured in M9 minimal medium (1) containing Na2HPO4 x 7H2O (6.8 g), KH2PO4 (3 g), NaCl (0.5 g), NH4Cl (1 g), MgSO4 (2 mmol), CaCl2 (0.1 mmol), trace elements, Wolf’s vitamin solution (2) and glucose (2 g L-1). Anoxic M9 minimal media with glucose was obtained by flushing solution with oxygen free nitrogen (95%). Overnight cultures from single colonies of each of seven E. coli strains were diluted to a starting optical density (OD600) of 0.01. Cultures were grown in 250 ml flasks or 300 ml oxygen-free sealed bottles containing 50 ml glucose-M9 minimal media in a shaking incubator at 37°C and 250 rpm. PGCGROWTHCONDITIONS SRR3194453 MCO000000245 glucose Medium supplement True
growth_protocol MCO Escherichia coli strains E.coli C ( DSMZ 4860 ) , E. coli Crooks ( DSMZ 1576 ) , E. coli DH5α ( DSMZ 6897 ) E. coli W ( DSMZ 1116 ) , E. coli W3110 ( DSMZ 5911 ) were obtained from DSMZ-German Collection of Microorganism and Cell Cultures ; E. coli BL21 ( DE3 ) was purchased as competent cells from Agilent ( Agilent Technologies Inc. , USA ) , E. coli K-12 MG1655 ( ATCC 700926 ) . All strains were cultured in <Med> M9 minimal medium </Med> ( 1 ) containing Na2HPO4 x 7H2O ( 6.8 g ) , KH2PO4 ( 3 g ) , NaCl ( 0.5 g ) , NH4Cl ( 1 g ) , MgSO4 ( 2 mmol ) , CaCl2 ( 0.1 mmol ) , trace elements , Wolf 's vitamin solution ( 2 ) and glucose ( 2 g L-1 ) . Anoxic <Med> M9 minimal media </Med> with <Supp> glucose </Supp> was obtained by flushing solution with oxygen free nitrogen ( 95 % ) . Overnight cultures from single colonies of each of seven E. coli strains were diluted to a starting optical density ( OD600 ) of 0.01 . Cultures were grown in 250 ml flasks or 300 ml oxygen-free sealed bottles containing 50 ml <Med> glucose-M9 minimal media </Med> in a shaking incubator at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> . GPL14548-GPL15010-GPL21530-GPL21531-GPL21532-GPL21533-GPL21534 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL21530: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. W3110). GPL21531: Illumina HiSeq 2000 (Escherichia coli W). GPL21532: Illumina HiSeq 2000 (Escherichia coli C). GPL21533: Illumina HiSeq 2000 (Escherichia coli BL21). GPL21534: Illumina HiSeq 2000 (Escherichia coli DH5[alpha]) GSE78756 Quantifying variation within the bacterial species E. coli GSM2075723 Crooks_anaero 27667363 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE78756/GSE78756.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Escherichia coli strains E.coli C (DSMZ 4860), E. coli Crooks (DSMZ 1576), E. coli DH5α (DSMZ 6897) E. coli W (DSMZ 1116), E. coli W3110 (DSMZ 5911) were obtained from DSMZ-German Collection of Microorganism and Cell Cultures; E. coli BL21 (DE3) was purchased as competent cells from Agilent (Agilent Technologies Inc., USA), E. coli K-12 MG1655 (ATCC 700926). All strains were cultured in M9 minimal medium (1) containing Na2HPO4 x 7H2O (6.8 g), KH2PO4 (3 g), NaCl (0.5 g), NH4Cl (1 g), MgSO4 (2 mmol), CaCl2 (0.1 mmol), trace elements, Wolf’s vitamin solution (2) and glucose (2 g L-1). Anoxic M9 minimal media with glucose was obtained by flushing solution with oxygen free nitrogen (95%). Overnight cultures from single colonies of each of seven E. coli strains were diluted to a starting optical density (OD600) of 0.01. Cultures were grown in 250 ml flasks or 300 ml oxygen-free sealed bottles containing 50 ml glucose-M9 minimal media in a shaking incubator at 37°C and 250 rpm. PGCGROWTHCONDITIONS SRR3194455SRR3194456 MCO000002526 M9 minimal medium Medium True
growth_protocol MCO Escherichia coli strains E.coli C ( DSMZ 4860 ) , E. coli Crooks ( DSMZ 1576 ) , E. coli DH5α ( DSMZ 6897 ) E. coli W ( DSMZ 1116 ) , E. coli W3110 ( DSMZ 5911 ) were obtained from DSMZ-German Collection of Microorganism and Cell Cultures ; E. coli BL21 ( DE3 ) was purchased as competent cells from Agilent ( Agilent Technologies Inc. , USA ) , E. coli K-12 MG1655 ( ATCC 700926 ) . All strains were cultured in <Med> M9 minimal medium </Med> ( 1 ) containing Na2HPO4 x 7H2O ( 6.8 g ) , KH2PO4 ( 3 g ) , NaCl ( 0.5 g ) , NH4Cl ( 1 g ) , MgSO4 ( 2 mmol ) , CaCl2 ( 0.1 mmol ) , trace elements , Wolf 's vitamin solution ( 2 ) and glucose ( 2 g L-1 ) . Anoxic <Med> M9 minimal media </Med> with <Supp> glucose </Supp> was obtained by flushing solution with oxygen free nitrogen ( 95 % ) . Overnight cultures from single colonies of each of seven E. coli strains were diluted to a starting optical density ( OD600 ) of 0.01 . Cultures were grown in 250 ml flasks or 300 ml oxygen-free sealed bottles containing 50 ml <Med> glucose-M9 minimal media </Med> in a shaking incubator at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> . GPL14548-GPL15010-GPL21530-GPL21531-GPL21532-GPL21533-GPL21534 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL21530: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. W3110). GPL21531: Illumina HiSeq 2000 (Escherichia coli W). GPL21532: Illumina HiSeq 2000 (Escherichia coli C). GPL21533: Illumina HiSeq 2000 (Escherichia coli BL21). GPL21534: Illumina HiSeq 2000 (Escherichia coli DH5[alpha]) GSE78756 Quantifying variation within the bacterial species E. coli GSM2075723 Crooks_anaero 27667363 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE78756/GSE78756.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt Escherichia coli strains E.coli C (DSMZ 4860), E. coli Crooks (DSMZ 1576), E. coli DH5α (DSMZ 6897) E. coli W (DSMZ 1116), E. coli W3110 (DSMZ 5911) were obtained from DSMZ-German Collection of Microorganism and Cell Cultures; E. coli BL21 (DE3) was purchased as competent cells from Agilent (Agilent Technologies Inc., USA), E. coli K-12 MG1655 (ATCC 700926). All strains were cultured in M9 minimal medium (1) containing Na2HPO4 x 7H2O (6.8 g), KH2PO4 (3 g), NaCl (0.5 g), NH4Cl (1 g), MgSO4 (2 mmol), CaCl2 (0.1 mmol), trace elements, Wolf’s vitamin solution (2) and glucose (2 g L-1). Anoxic M9 minimal media with glucose was obtained by flushing solution with oxygen free nitrogen (95%). Overnight cultures from single colonies of each of seven E. coli strains were diluted to a starting optical density (OD600) of 0.01. Cultures were grown in 250 ml flasks or 300 ml oxygen-free sealed bottles containing 50 ml glucose-M9 minimal media in a shaking incubator at 37°C and 250 rpm. PGCGROWTHCONDITIONS SRR3194455SRR3194456 MCO000000245 glucose Medium supplement True
growth_protocol MCO LB medium , 180 rpm shaking , at <Temp> 37 °C </Temp> , between exponential and <Phase> stationary phase </Phase> GPL23073 GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933) GSE94984 Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP GSM2493797 EHEC in LB Experiment 2 [RNA-Seq] 28245801 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE94984/GSE94984.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt LB medium, 180 rpm shaking, at 37°C, between exponential and stationary phase PGCGROWTHCONDITIONS SRR5266619 MCO000002866 stationary phase Growth phase True
source_name MCO biomass collected in the transition between exponential to <Phase> stationary phase </Phase> GPL23073 GPL23073: Illumina MiSeq (Escherichia coli O157:H7 str. EDL933) GSE94984 Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP GSM2493797 EHEC in LB Experiment 2 [RNA-Seq] 28245801 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE94984/GSE94984.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt biomass collected in the transition between exponential to stationary phase PGCGROWTHCONDITIONS SRR5266619 MCO000002866 stationary phase Growth phase True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE95318 RNA-sequencing identifies new RNase III cleavage sites in E. coli and reveals increased regulation of mRNA GSM2507165 WTA_time0 28351917 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95318/GSE95318.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR5282299 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE95318 RNA-sequencing identifies new RNase III cleavage sites in E. coli and reveals increased regulation of mRNA GSM2507166 WTA_time2.5 28351917 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95318/GSE95318.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR5282300 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE95318 RNA-sequencing identifies new RNase III cleavage sites in E. coli and reveals increased regulation of mRNA GSM2507167 WTA_time5 28351917 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95318/GSE95318.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR5282301 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE95318 RNA-sequencing identifies new RNase III cleavage sites in E. coli and reveals increased regulation of mRNA GSM2507168 WTA_time7.5 28351917 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95318/GSE95318.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR5282302 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE95318 RNA-sequencing identifies new RNase III cleavage sites in E. coli and reveals increased regulation of mRNA GSM2507169 WTA_time10 28351917 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95318/GSE95318.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR5282303 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE95318 RNA-sequencing identifies new RNase III cleavage sites in E. coli and reveals increased regulation of mRNA GSM2507170 WTA_time20 28351917 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95318/GSE95318.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR5282304 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE95318 RNA-sequencing identifies new RNase III cleavage sites in E. coli and reveals increased regulation of mRNA GSM2507171 WTB_time0 28351917 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95318/GSE95318.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR5282305 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE95318 RNA-sequencing identifies new RNase III cleavage sites in E. coli and reveals increased regulation of mRNA GSM2507172 WTB_time2.5 28351917 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95318/GSE95318.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR5282306 MCO000002470 wild type Genetic background True
characteristics MCO genotype/variation : <Gtype> wild type </Gtype> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE95318 RNA-sequencing identifies new RNase III cleavage sites in E. coli and reveals increased regulation of mRNA GSM2507173 WTB_time7.5 28351917 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE95318/GSE95318.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/gc_ontology_terms_v2.txt genotype/variation: wild type PGCGROWTHCONDITIONS SRR5282307 MCO000002470 wild type Genetic background True
characteristics MCO wild type genotype : <Gtype> wildtype </Gtype> GPL21222-GPL21726 GPL21222: Illumina NextSeq 500 (Escherichia coli). GPL21726: Ion Torrent Proton (Escherichia coli) GSE104504 Crosstalk between global regulators CRP and FIS in E. coli. GSM2802566 wt.rep1.me 29205228 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE104504/GSE104504.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: wildtype PGCGROWTHCONDITIONS SRR6125557 MCO000002470 wildtype Genetic background True
characteristics MCO wild type genotype : <Gtype> wildtype </Gtype> GPL21222-GPL21726 GPL21222: Illumina NextSeq 500 (Escherichia coli). GPL21726: Ion Torrent Proton (Escherichia coli) GSE104504 Crosstalk between global regulators CRP and FIS in E. coli. GSM2802567 wt.rep2.me 29205228 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE104504/GSE104504.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: wildtype PGCGROWTHCONDITIONS SRR6125558 MCO000002470 wildtype Genetic background True
source_name MCO exponential phase bacteria grown at 37 ° C with shaking until <Phase> log phase </Phase> GPL10328 GPL10328: Illumina Genome Analyzer II (Escherichia coli) GSE36248 Transcriptome mapping of blaCMY-2 positive IncA/C plasmid pAR060302 GSM885047 no treatment http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE36248/GSE36248.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json bacteria grown at 37° C with shaking until log phase PGCGROWTHCONDITIONS SRR427120 MCO000002864 log phase Growth phase True
growth_protocol MCO agitation at 250 rpm Cells were grown in a 250 ml fermenter containing 100 ml LB medium supplemented with 3 g/L glucose , <Supp> 0.1 mM IPTG , </Supp> and 50 μg/mL antibiotics . The fermentors were operated at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> with aeration ( 200 mL/min ) . Flow rate was 1.162 ml/min ( dilution rate 0,7 / h ) . The pellets were used for RNA . GPL16760 GPL16760: Illumina Genome Analyzer II (Escherichia coli BL21(DE3)) GSE44928 Physiology study of Escherichia coli harboring high intracellular ATP driven by artificial Pck expression GSM1094082 BL21_2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE44928/GSE44928.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Cells were grown in a 250 ml fermenter containing 100 ml LB medium supplemented with 3 g/L glucose, 0.1 mM IPTG, and 50 μg/mL antibiotics. The fermentors were operated at 37 °C and 250 rpm with aeration (200 mL/min). Flow rate was 1.162ml/min(dilution rate 0,7/h). The pellets were used for RNA. PGCGROWTHCONDITIONS SRR771533 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Cells were grown in a 250 ml fermenter containing 100 ml LB medium supplemented with 3 g/L glucose , <Supp> 0.1 mM IPTG , </Supp> and 50 μg/mL antibiotics . The fermentors were operated at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> with aeration ( 200 mL/min ) . Flow rate was 1.162 ml/min ( dilution rate 0,7 / h ) . The pellets were used for RNA . GPL16760 GPL16760: Illumina Genome Analyzer II (Escherichia coli BL21(DE3)) GSE44928 Physiology study of Escherichia coli harboring high intracellular ATP driven by artificial Pck expression GSM1094083 PCK over http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE44928/GSE44928.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Cells were grown in a 250 ml fermenter containing 100 ml LB medium supplemented with 3 g/L glucose, 0.1 mM IPTG, and 50 μg/mL antibiotics. The fermentors were operated at 37 °C and 250 rpm with aeration (200 mL/min). Flow rate was 1.162ml/min(dilution rate 0,7/h). The pellets were used for RNA. PGCGROWTHCONDITIONS SRR771534 MCO000011588 250 rpm Agitation speed True
characteristics MCO wild type genotype : <Gtype> wt </Gtype> GPL18814 GPL18814: Illumina HiSeq 2000 (Escherichia coli BW38028) GSE58556 RNA sequencing based analysis of the bacterial transcriptome GSM1413874 WT_glucose_log 25483350 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE58556/GSE58556.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: wt PGCGROWTHCONDITIONS SRR1411272 MCO000002470 wt Genetic background True
characteristics MCO wild type strain : <Gtype> wt </Gtype> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE64848 Integrated analysis of molecular and systems level function of the Crp regulon [RNA-Seq] GSM1581598 RNAseq_wt_fructose_NH4Cl_O2_1 29771928 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE64848/GSE64848.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json strain: wt PGCGROWTHCONDITIONS SRR1751336SRR1751337SRR1751338 MCO000002470 wt Genetic background True
characteristics MCO wild type strain : <Gtype> wt </Gtype> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE64848 Integrated analysis of molecular and systems level function of the Crp regulon [RNA-Seq] GSM1581599 RNAseq_wt_fructose_NH4Cl_O2_2 29771928 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE64848/GSE64848.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json strain: wt PGCGROWTHCONDITIONS SRR1751339SRR1751340SRR1751341 MCO000002470 wt Genetic background True
characteristics MCO wild type strain : <Gtype> wt </Gtype> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE64848 Integrated analysis of molecular and systems level function of the Crp regulon [RNA-Seq] GSM1581600 RNAseq_wt_glucose_NH4Cl_O2_1 29771928 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE64848/GSE64848.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json strain: wt PGCGROWTHCONDITIONS SRR1751342SRR1751343 MCO000002470 wt Genetic background True
characteristics MCO wild type strain : <Gtype> wt </Gtype> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE64848 Integrated analysis of molecular and systems level function of the Crp regulon [RNA-Seq] GSM1581601 RNAseq_wt_glucose_NH4Cl_O2_2 29771928 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE64848/GSE64848.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json strain: wt PGCGROWTHCONDITIONS SRR1751344SRR1751345 MCO000002470 wt Genetic background True
characteristics MCO wild type strain : <Gtype> wt </Gtype> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE64848 Integrated analysis of molecular and systems level function of the Crp regulon [RNA-Seq] GSM1581602 RNAseq_wt_glucose_NH4Cl_O2_3 29771928 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE64848/GSE64848.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json strain: wt PGCGROWTHCONDITIONS SRR1751346 MCO000002470 wt Genetic background True
characteristics MCO wild type strain : <Gtype> wt </Gtype> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE64848 Integrated analysis of molecular and systems level function of the Crp regulon [RNA-Seq] GSM1581603 RNAseq_wt_glycerol_NH4Cl_O2_1 29771928 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE64848/GSE64848.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json strain: wt PGCGROWTHCONDITIONS SRR1751347SRR1751348SRR1751349 MCO000002470 wt Genetic background True
characteristics MCO wild type strain : <Gtype> wt </Gtype> GPL16085 GPL16085: Illumina MiSeq (Escherichia coli) GSE64848 Integrated analysis of molecular and systems level function of the Crp regulon [RNA-Seq] GSM1581604 RNAseq_wt_glycerol_NH4Cl_O2_2 29771928 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE64848/GSE64848.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json strain: wt PGCGROWTHCONDITIONS SRR1751350SRR1751351SRR1751352 MCO000002470 wt Genetic background True
characteristics MCO wild type genotype : <Gtype> wildtype </Gtype> GPL14548 GPL14548: Illumina HiSeq 2000 (Escherichia coli) GSE65244 Temporal gene expression in Escherichia coli GSM1590712 Wt – 60 min http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE65244/GSE65244.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json genotype: wildtype PGCGROWTHCONDITIONS SRR1771414 MCO000002470 wildtype Genetic background True
growth_protocol MCO minimal defined medium Glycerol stocks of E. coli K12 strain MG1655 were inoculated into LB media grown at 37 ℃ with constant agitation overnight . Cultures were diluted 1:100 into fresh <Med> minimal medium </Med> and then cultured at 37 ℃ to mid-exponential phase ( OD600 nm ~ 0.6 ) . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE67218 Transcriptional expression level of E. coli at 37 ℃ in LB media GSM1642593 RNA-seq 37C LB rep1 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE67218/GSE67218.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Glycerol stocks of E. coli K12 strain MG1655 were inoculated into LB media grown at 37 ℃ with constant agitation overnight. Cultures were diluted 1:100 into fresh minimal medium and then cultured at 37 ℃ to mid-exponential phase (OD600 nm ~ 0.6). PGCGROWTHCONDITIONS SRR1927169 MCO000011586 minimal medium Medium True
growth_protocol MCO minimal defined medium Glycerol stocks of E. coli K12 strain MG1655 were inoculated into LB media grown at 37 ℃ with constant agitation overnight . Cultures were diluted 1:100 into fresh <Med> minimal medium </Med> and then cultured at 37 ℃ to mid-exponential phase ( OD600 nm ~ 0.6 ) . GPL17439 GPL17439: Illumina MiSeq (Escherichia coli str. K-12 substr. MG1655) GSE67218 Transcriptional expression level of E. coli at 37 ℃ in LB media GSM1642594 RNA-seq 37C LB rep2 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE67218/GSE67218.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Glycerol stocks of E. coli K12 strain MG1655 were inoculated into LB media grown at 37 ℃ with constant agitation overnight. Cultures were diluted 1:100 into fresh minimal medium and then cultured at 37 ℃ to mid-exponential phase (OD600 nm ~ 0.6). PGCGROWTHCONDITIONS SRR1927170 MCO000011586 minimal medium Medium True
characteristics MCO exponential phase growth phase : <Phase> log phase </Phase> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE76167 Mechanisms of nickel toxicity in bacteria GSM1975603 Rodrigue_1-WT-phiNi-1 2737513027668277 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE76167/GSE76167.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json growth phase: log phase PGCGROWTHCONDITIONS SRR3033275SRR3033276SRR3033277SRR3033278SRR3033279 MCO000002864 log phase Growth phase True
characteristics MCO exponential phase growth phase : <Phase> log phase </Phase> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE76167 Mechanisms of nickel toxicity in bacteria GSM1975604 Rodrigue_6-WT-phiNi-2 2737513027668277 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE76167/GSE76167.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json growth phase: log phase PGCGROWTHCONDITIONS SRR3033280SRR3033281SRR3033282SRR3033283 MCO000002864 log phase Growth phase True
characteristics MCO exponential phase growth phase : <Phase> log phase </Phase> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE76167 Mechanisms of nickel toxicity in bacteria GSM1975605 Rodrigue_9-WT-phiNi-3 2737513027668277 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE76167/GSE76167.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json growth phase: log phase PGCGROWTHCONDITIONS SRR3033284SRR3033285SRR3033286SRR3033287 MCO000002864 log phase Growth phase True
characteristics MCO exponential phase growth phase : <Phase> log phase </Phase> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE76167 Mechanisms of nickel toxicity in bacteria GSM1975606 Rodrigue_2-WT-Ni-1 2737513027668277 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE76167/GSE76167.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json growth phase: log phase PGCGROWTHCONDITIONS SRR3033288SRR3033289SRR3033290SRR3033291SRR3033292 MCO000002864 log phase Growth phase True
characteristics MCO exponential phase growth phase : <Phase> log phase </Phase> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE76167 Mechanisms of nickel toxicity in bacteria GSM1975607 Rodrigue_5-WT-Ni-2 2737513027668277 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE76167/GSE76167.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json growth phase: log phase PGCGROWTHCONDITIONS SRR3033293SRR3033294SRR3033295SRR3033296 MCO000002864 log phase Growth phase True
characteristics MCO exponential phase growth phase : <Phase> log phase </Phase> GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE76167 Mechanisms of nickel toxicity in bacteria GSM1975608 Rodrigue_10-WT-Ni-3 2737513027668277 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE76167/GSE76167.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json growth phase: log phase PGCGROWTHCONDITIONS SRR3033297SRR3033298SRR3033299SRR3033300SRR3033301 MCO000002864 log phase Growth phase True
growth_protocol MCO agitation at 250 rpm Escherichia coli strains E.coli C ( DSMZ 4860 ) , E. coli Crooks ( DSMZ 1576 ) , E. coli DH5α ( DSMZ 6897 ) E. coli W ( DSMZ 1116 ) , E. coli W3110 ( DSMZ 5911 ) were obtained from DSMZ-German Collection of Microorganism and Cell Cultures ; E. coli BL21 ( DE3 ) was purchased as competent cells from Agilent ( Agilent Technologies Inc. , USA ) , E. coli K-12 MG1655 ( ATCC 700926 ) . All strains were cultured in <Med> M9 minimal medium </Med> ( 1 ) containing Na2HPO4 x 7H2O ( 6.8 g ) , KH2PO4 ( 3 g ) , NaCl ( 0.5 g ) , NH4Cl ( 1 g ) , MgSO4 ( 2 mmol ) , CaCl2 ( 0.1 mmol ) , trace elements , Wolf 's vitamin solution ( 2 ) and glucose ( 2 g L-1 ) . Anoxic <Med> M9 minimal media </Med> with <Supp> glucose </Supp> was obtained by flushing solution with oxygen free nitrogen ( 95 % ) . Overnight cultures from single colonies of each of seven E. coli strains were diluted to a starting optical density ( OD600 ) of 0.01 . Cultures were grown in 250 ml flasks or 300 ml oxygen-free sealed bottles containing 50 ml <Med> glucose-M9 minimal media </Med> in a shaking incubator at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> . GPL14548-GPL15010-GPL21530-GPL21531-GPL21532-GPL21533-GPL21534 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL21530: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. W3110). GPL21531: Illumina HiSeq 2000 (Escherichia coli W). GPL21532: Illumina HiSeq 2000 (Escherichia coli C). GPL21533: Illumina HiSeq 2000 (Escherichia coli BL21). GPL21534: Illumina HiSeq 2000 (Escherichia coli DH5[alpha]) GSE78756 Quantifying variation within the bacterial species E. coli GSM2075722 Crooks_aero 27667363 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE78756/GSE78756.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Escherichia coli strains E.coli C (DSMZ 4860), E. coli Crooks (DSMZ 1576), E. coli DH5α (DSMZ 6897) E. coli W (DSMZ 1116), E. coli W3110 (DSMZ 5911) were obtained from DSMZ-German Collection of Microorganism and Cell Cultures; E. coli BL21 (DE3) was purchased as competent cells from Agilent (Agilent Technologies Inc., USA), E. coli K-12 MG1655 (ATCC 700926). All strains were cultured in M9 minimal medium (1) containing Na2HPO4 x 7H2O (6.8 g), KH2PO4 (3 g), NaCl (0.5 g), NH4Cl (1 g), MgSO4 (2 mmol), CaCl2 (0.1 mmol), trace elements, Wolf’s vitamin solution (2) and glucose (2 g L-1). Anoxic M9 minimal media with glucose was obtained by flushing solution with oxygen free nitrogen (95%). Overnight cultures from single colonies of each of seven E. coli strains were diluted to a starting optical density (OD600) of 0.01. Cultures were grown in 250 ml flasks or 300 ml oxygen-free sealed bottles containing 50 ml glucose-M9 minimal media in a shaking incubator at 37°C and 250 rpm. PGCGROWTHCONDITIONS SRR3194453 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Escherichia coli strains E.coli C ( DSMZ 4860 ) , E. coli Crooks ( DSMZ 1576 ) , E. coli DH5α ( DSMZ 6897 ) E. coli W ( DSMZ 1116 ) , E. coli W3110 ( DSMZ 5911 ) were obtained from DSMZ-German Collection of Microorganism and Cell Cultures ; E. coli BL21 ( DE3 ) was purchased as competent cells from Agilent ( Agilent Technologies Inc. , USA ) , E. coli K-12 MG1655 ( ATCC 700926 ) . All strains were cultured in <Med> M9 minimal medium </Med> ( 1 ) containing Na2HPO4 x 7H2O ( 6.8 g ) , KH2PO4 ( 3 g ) , NaCl ( 0.5 g ) , NH4Cl ( 1 g ) , MgSO4 ( 2 mmol ) , CaCl2 ( 0.1 mmol ) , trace elements , Wolf 's vitamin solution ( 2 ) and glucose ( 2 g L-1 ) . Anoxic <Med> M9 minimal media </Med> with <Supp> glucose </Supp> was obtained by flushing solution with oxygen free nitrogen ( 95 % ) . Overnight cultures from single colonies of each of seven E. coli strains were diluted to a starting optical density ( OD600 ) of 0.01 . Cultures were grown in 250 ml flasks or 300 ml oxygen-free sealed bottles containing 50 ml <Med> glucose-M9 minimal media </Med> in a shaking incubator at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> . GPL14548-GPL15010-GPL21530-GPL21531-GPL21532-GPL21533-GPL21534 GPL14548: Illumina HiSeq 2000 (Escherichia coli). GPL15010: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. MG1655). GPL21530: Illumina HiSeq 2000 (Escherichia coli str. K-12 substr. W3110). GPL21531: Illumina HiSeq 2000 (Escherichia coli W). GPL21532: Illumina HiSeq 2000 (Escherichia coli C). GPL21533: Illumina HiSeq 2000 (Escherichia coli BL21). GPL21534: Illumina HiSeq 2000 (Escherichia coli DH5[alpha]) GSE78756 Quantifying variation within the bacterial species E. coli GSM2075723 Crooks_anaero 27667363 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE78756/GSE78756.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Escherichia coli strains E.coli C (DSMZ 4860), E. coli Crooks (DSMZ 1576), E. coli DH5α (DSMZ 6897) E. coli W (DSMZ 1116), E. coli W3110 (DSMZ 5911) were obtained from DSMZ-German Collection of Microorganism and Cell Cultures; E. coli BL21 (DE3) was purchased as competent cells from Agilent (Agilent Technologies Inc., USA), E. coli K-12 MG1655 (ATCC 700926). All strains were cultured in M9 minimal medium (1) containing Na2HPO4 x 7H2O (6.8 g), KH2PO4 (3 g), NaCl (0.5 g), NH4Cl (1 g), MgSO4 (2 mmol), CaCl2 (0.1 mmol), trace elements, Wolf’s vitamin solution (2) and glucose (2 g L-1). Anoxic M9 minimal media with glucose was obtained by flushing solution with oxygen free nitrogen (95%). Overnight cultures from single colonies of each of seven E. coli strains were diluted to a starting optical density (OD600) of 0.01. Cultures were grown in 250 ml flasks or 300 ml oxygen-free sealed bottles containing 50 ml glucose-M9 minimal media in a shaking incubator at 37°C and 250 rpm. PGCGROWTHCONDITIONS SRR3194455SRR3194456 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349915 Tube state 1 (IPTG-/aTc-/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427755 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349916 Tube state 2 (IPTG+/aTc-/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427756 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349917 Tube state 3 (IPTG-/aTc+/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427757 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349918 Tube state 4 (IPTG+/aTc+/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427758 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349919 Tube state 5 (IPTG-/aTc-/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427759 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349920 Tube state 6 (IPTG+/aTc-/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427760 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349921 Tube state 7 (IPTG-/aTc+/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427761 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349922 Tube state 8 (IPTG+/aTc+/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427762 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349923 Flask state 1 (IPTG-/aTc-/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427763 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349924 Flask state 2 (IPTG+/aTc-/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427764 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349925 Flask state 3 (IPTG-/aTc+/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427765 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349926 Flask state 4 (IPTG+/aTc+/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427766 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349927 Flask state 5 (IPTG-/aTc-/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427767 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349928 Flask state 6 (IPTG+/aTc-/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427768 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349929 Flask state 7 (IPTG-/aTc+/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427769 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . For culture tube assays ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) , cells were diluted 658-fold ( 4.56 µL into 3 mL ) into EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE88835 Genetic circuit 0x58 GSM2349930 Flask state 8 (IPTG+/aTc+/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE88835/GSE88835.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. For culture tube assays (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059), cells were diluted 658-fold (4.56 µL into 3 mL) into EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR4427770 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757255 0x58 replicate 2 state 1 (IPTG-/aTc-/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985582 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757256 0x58 replicate 2 state 2 (IPTG+/aTc-/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985583 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757257 0x58 replicate 2 state 3 (IPTG-/aTc+/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985584 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757258 0x58 replicate 2 state 4 (IPTG+/aTc+/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985585 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757259 0x58 replicate 2 state 5 (IPTG-/aTc-/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985586 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757260 0x58 replicate 2 state 6 (IPTG+/aTc-/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985587 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757261 0x58 replicate 2 state 7 (IPTG-/aTc+/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985588 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757262 0x58 replicate 2 state 8 (IPTG+/aTc+/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985589 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757263 0x58 replicate 3 state 1 (IPTG-/aTc-/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985590 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757264 0x58 replicate 3 state 2 (IPTG+/aTc-/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985591 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757265 0x58 replicate 3 state 3 (IPTG-/aTc+/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985592 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757266 0x58 replicate 3 state 4 (IPTG+/aTc+/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985593 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757267 0x58 replicate 3 state 5 (IPTG-/aTc-/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985594 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757268 0x58 replicate 3 state 6 (IPTG+/aTc-/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985595 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757269 0x58 replicate 3 state 7 (IPTG-/aTc+/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985596 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757270 0x58 replicate 3 state 8 (IPTG+/aTc+/Ara+) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985597 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757272 Control pAN1201 replicate 1 (IPTG-/aTc-/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985599 MCO000011588 250 rpm Agitation speed True
growth_protocol MCO agitation at 250 rpm Individual colonies were inoculated into MOPS EZ Rich Defined Medium ( Teknova , CA , M2105 ) with 0.2 % glycerol carbon source and 50 μg/mL kanamycin ( Gold Biotechnology , MO , K-120-5 ) and grown overnight for <Supp> 16 hours </Supp> at <Temp> 37 °C </Temp> and 1000 RPM in V-bottom 96-well plates ( Nunc , Roskilde , Denmark , 249952 ) in an ELMI Digital Thermos Microplates shaker incubator ( Elmi Ltd , Riga , Latvia ) . The following day , cultures were diluted 178-fold ( two serial dilutions of 15 µL into 185 µL ) into EZ Rich glycerol with kanamycin , and grown under the same ELMI shaker incubator conditions for three hours . Cells were diluted 658-fold ( 4.56 µL into 3 mL ) into culture tubes ( Falcon 14 mL round-bottom polypropylene tubes ; Corning , MA , 352059 ) containing EZ Rich glycerol with kanamycin and inducers . For Erlenmeyer flask assays ( Pyrex 250 mL ; Cole-Palmer , IL , 4980-250 ) , cells were diluted 658-fold ( 76 µL into 50 mL ) into EZ Rich glycerol with kanamycin and inducers . Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG , 10 ng/mL aTc , and 5 mM L-arabinose . Culture tubes were then grown in an Innova 44 shaker ( Eppendorf , CT ) at <Temp> 37 °C </Temp> and <Agit> 250 rpm </Agit> for five hours . GPL18133 GPL18133: Illumina HiSeq 2500 (Escherichia coli) GSE98890 Genetic circuit 0x58 replicates and modified GSM2757273 Control pAN1201 replicate 2 (IPTG-/aTc-/Ara-) 29122925 http://pakal.ccg.unam.mx/cmendezc/automatic-extraction-growth-conditions/tree/master/extraction-geo/download/srr_htregulondb/GSE98890/GSE98890.soft.gz 100 100 /home/egaytan/automatic-extraction-growth-conditions/mapping_MCO/input/mco_terms_v0.2.json Individual colonies were inoculated into MOPS EZ Rich Defined Medium (Teknova, CA, M2105) with 0.2% glycerol carbon source and 50 μg/mL kanamycin (Gold Biotechnology, MO, K-120-5) and grown overnight for 16 hours at 37 °C and 1000 RPM in V-bottom 96-well plates (Nunc, Roskilde, Denmark, 249952) in an ELMI Digital Thermos Microplates shaker incubator (Elmi Ltd, Riga, Latvia). The following day, cultures were diluted 178-fold (two serial dilutions of 15 µL into 185 µL) into EZ Rich glycerol with kanamycin, and grown under the same ELMI shaker incubator conditions for three hours. Cells were diluted 658-fold (4.56 µL into 3 mL) into culture tubes (Falcon 14 mL round-bottom polypropylene tubes; Corning, MA, 352059) containing EZ Rich glycerol with kanamycin and inducers. For Erlenmeyer flask assays (Pyrex 250 mL; Cole-Palmer, IL, 4980-250), cells were diluted 658-fold (76 µL into 50 mL) into EZ Rich glycerol with kanamycin and inducers. Eight inducer combinations were used that cover the presence or absence of 0.5 mM IPTG, 10 ng/mL aTc, and 5 mM L-arabinose. Culture tubes were then grown in an Innova 44 shaker (Eppendorf, CT) at 37 °C and 250 rpm for five hours. PGCGROWTHCONDITIONS SRR5985600 MCO000011588 250 rpm Agitation speed True