GSE9923-GSM247668-GPL199-GPL3154-PMID:18528414.tsv 1.07 KB
"RNA extracted from free living cells of E. coli K-12 BW25113 luxS mutant (when growth to OD600 0.5 at 37ºC in LB, 100 uM AI2 was added in and 3-h further incubation was applied)"	"characteristics_ch1.1"
"MAS 5.0 Expression Analysis Default Setting"	"data_processing.1"
"The overnight culture (0.25 ml) was used to inoculate 25 ml of fresh LB medium. After incubation at 37°C with shaking (250 rpm) to OD600 0.5, 100 uM AI-2 was added in. after further 3-h incubation, 2.0 ml of cell culture was removed and cells were pelleted and frozen in -80C. After breaking the cells with a bead beater, and the total RNA was isolated with Qiagen RNeasy mini Kit (Cat# 74104)"	"extract_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Channel 1"	"source_name_ch1.1"
"BW25113 luxS LB 37C free-living cells 100 uM AI2 3h"	"title.1"
"RNA extracted from free living cells of E. coli K-12 BW25113 luxS mutant (when growth to OD600 0.5 at 37ºC in LB, 100 uM AI2 was added in and 3-h further incubation was applied)"	"characteristics_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Channel 1"	"source_name_ch1.1"