GSE96955-GSM2546594-GPL3154-PMID:29875739.tsv 1.8 KB
"genotyp/variation: deltacrp JW5702-4 background with a K100R mutant of CRP (CRP R) inserted into paaH gene locus"	"characteristics_ch1.1"
"Data were analyzed with RMA (Robust Multiarray Analysis) using Affymetrix default analysis settings and global scaling as normalization method."	"data_processing.1"
"Total RNA extraction using Vantage™ RNA Purification Kit (Origene, Rockville, MD, USA) was performed according with the manufacter instructions.  Purity and concentration of isolated RNA were assessed in a NanoDrop One spectrophotometer (Thermo Scientific Incorporated, WI, USA). Quality was evaluated by microfluidic capillary electrophoresis on an Agilent 2100 Bioanalyzer (Agilent Technologies, Inc, USA)."	"extract_protocol_ch1.1"
"E. coli Wild type and its mutants were grown in M9 minimal medium with 10mM glucose or 30mM acetate as sole carbon source. Samples for RNA extraction were taken in middle exponential phase (OD600≈0.5) and in stationary phase (OD600≈1.5)"	"growth_protocol_ch1.1"
"Escherichia coli K-12"	"organism_ch1.1"
"E. coli CRP R strain grown in glucose batch minimal media, cells were harvested at stationary phase, biological rep2"	"source_name_ch1.1"
"E. coli CRP R strain_glucose_stationary phase_repl2"	"title.1"
"genotyp/variation: deltacrp JW5702-4 background with a K100R mutant of CRP (CRP R) inserted into paaH gene locus"	"characteristics_ch1.1"
"E. coli Wild type and its mutants were grown in M9 minimal medium with 10mM glucose or 30mM acetate as sole carbon source. Samples for RNA extraction were taken in middle exponential phase (OD600≈0.5) and in stationary phase (OD600≈1.5)"	"growth_protocol_ch1.1"
"Escherichia coli K-12"	"organism_ch1.1"
"E. coli CRP R strain grown in glucose batch minimal media, cells were harvested at stationary phase, biological rep2"	"source_name_ch1.1"