GSE94342-GSM2473483-GPL13360-PMID:28952540.tsv
2.08 KB
"protocol: M9" "characteristics_ch1.1"
"genotype: rpoC K370_A396dup mutant" "characteristics_ch1.2"
"strain: BW25113" "characteristics_ch1.3"
"protocol: M9" "characteristics_ch2.1"
"genotype: Wild-type strain" "characteristics_ch2.2"
"strain: BW25113" "characteristics_ch2.3"
"Data were normalized using the LOWESS algorithm and analyzed for statistical significance using the MeV package" "data_processing.1"
"Samples were grown to mid exponential phase (OD approx 0.5). Cultures were then quickly chilled to ≤ 4 ºC on dry ice/isopropanol bath, harvested by centrifugation (4470 × g) at 4 ºC and resuspended in RNALater to stop RNA degradation. The Zymo Quick-RNA miniprep kit was used to extract pure RNA for subsequent analysis" "extract_protocol_ch1.1"
"Samples were grown to mid exponential phase (OD approx 0.5). Cultures were then quickly chilled to ≤ 4 ºC on dry ice/isopropanol bath, harvested by centrifugation (4470 × g) at 4 ºC and resuspended in RNALater to stop RNA degradation. The Zymo Quick-RNA miniprep kit was used to extract pure RNA for subsequent analysis" "extract_protocol_ch2.1"
"Grown in glucose M9 media with or without supplementation of 0.6 M NaCl" "growth_protocol_ch1.1"
"Grown in glucose M9 media with or without supplementation of 0.6 M NaCl" "growth_protocol_ch2.1"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli" "organism_ch2.1"
"EJW3, BW25113 rpoC K370_A396dup" "source_name_ch1.1"
"BW25113" "source_name_ch2.1"
"EJW3 in M9 Replicate 1" "title.1"
"protocol: M9" "characteristics_ch1.1"
"genotype: rpoC K370_A396dup mutant" "characteristics_ch1.2"
"strain: BW25113" "characteristics_ch1.3"
"protocol: M9" "characteristics_ch2.1"
"genotype: Wild-type strain" "characteristics_ch2.2"
"strain: BW25113" "characteristics_ch2.3"
"Grown in glucose M9 media with or without supplementation of 0.6 M NaCl" "growth_protocol_ch1.1"
"Grown in glucose M9 media with or without supplementation of 0.6 M NaCl" "growth_protocol_ch2.1"
"Escherichia coli" "organism_ch1.1"
"Escherichia coli" "organism_ch2.1"
"EJW3, BW25113 rpoC K370_A396dup" "source_name_ch1.1"
"BW25113" "source_name_ch2.1"