GSE94117-GSM2469653-GPL22993-PMID:30388153.tsv 1.82 KB
"growth time (hours): 9"	"characteristics_ch1.1"
"growth phase: exponential"	"characteristics_ch1.2"
"carbon source: lactate"	"characteristics_ch1.3"
"mg+2 (mm): 0.8"	"characteristics_ch1.4"
"mg+2 (levels): baseMg"	"characteristics_ch1.5"
"na+1 (mm): 5"	"characteristics_ch1.6"
"na+1 (levels): baseNa"	"characteristics_ch1.7"
"batch: 16"	"characteristics_ch1.8"
"trim adaptors on raw reads and moved trimmed reads to trimmed_reads with flexbar"	"data_processing.1"
"bowtie2-build final_reference_seqs/REL606.fa indexes/REL606"	"data_processing.2"
"map trimmed reads to reference sequence in indexes file with bowtie2"	"data_processing.3"
"convert bowtie output .sam to .bam with samtools"	"data_processing.4"
"convert back to sorted.sam with samtools"	"data_processing.5"
"get raw counts for reads mapped with htseq-count"	"data_processing.6"
"Genome_build: reference sequence is REL606"	"data_processing.7"
"Supplementary_files_format_and_content: txt files including count numbers of RNA's and quality control were generated"	"data_processing.8"
"RNAsnap followed by purification through Zymo RNA clean and concentrator."	"extract_protocol_ch1.1"
"Illumina Ribozero rRNA removal followed by NEB Next RNA library Prep set."	"extract_protocol_ch1.2"
"Escherichia coli B str. REL606"	"organism_ch1.1"
"exponential culture"	"source_name_ch1.1"
"lactate_growth: MURI_085"	"title.1"
"RNA-Seq"	"library_strategy.1"
"growth time (hours): 9"	"characteristics_ch1.1"
"growth phase: exponential"	"characteristics_ch1.2"
"carbon source: lactate"	"characteristics_ch1.3"
"mg+2 (mm): 0.8"	"characteristics_ch1.4"
"mg+2 (levels): baseMg"	"characteristics_ch1.5"
"na+1 (mm): 5"	"characteristics_ch1.6"
"na+1 (levels): baseNa"	"characteristics_ch1.7"
"batch: 16"	"characteristics_ch1.8"
"Escherichia coli B str. REL606"	"organism_ch1.1"
"exponential culture"	"source_name_ch1.1"