GSE93506-GSM2453487-GPL16227-PMID:28061857.tsv
1.95 KB
"strain: MG1655" "characteristics_ch1.1"
"transcription factor: Nac" "characteristics_ch1.2"
"Basecalls performed using CASAVA version 1.8" "data_processing.1"
"Reads were aligned to GenBank ID U00096.2" "data_processing.2"
"Peak calling was done using SPAT" "data_processing.3"
"R scripts (Bioconductor GenomicRanges) and custom scripts were used to calculate RPKMs. Fold-change was calculated as ratio of RPKMs after TF induction to control experiment." "data_processing.4"
"Genome_build: U00096.2 (GenBank)" "data_processing.5"
"Supplementary_files_format_and_content: .xlsx files contain peak region locations and gene expression value for ChIP-Seq and RNA-Seq respectively" "data_processing.6"
"For chromatin immunoprecipitation, cells were fixed with formaldehyde and glycine and sheared via sonication. Sheared DNA from lysate was immunoprecipitated (IP) out using an anti-FLAG mAb (Sigma). DNA was washed with IPP150 buffer and purified via ProteinaseK digestion and Qiaquick PCR purification kit (Qiagen - Cat. No. 28106)." "extract_protocol_ch1.1"
"Total RNA extraction was done using TRIzol reagent and DNAse digested. RNA purfication was carried out using Rneasy spin columns" "extract_protocol_ch1.2"
"The appropriate protocols were performed using standard Illumina procedures" "extract_protocol_ch1.3"
"Cells were grown in LB media with 1mM IPTG at 37 °C with shaking for 2 hours" "growth_protocol_ch1.1"
"Escherichia coli K-12" "organism_ch1.1"
"Culture cells" "source_name_ch1.1"
"Nac_RNASeq" "title.1"
"For total RNA extraction, cells were pelleted in 4 °C." "treatment_protocol_ch1.1"
"RNA-Seq" "library_strategy.1"
"strain: MG1655" "characteristics_ch1.1"
"transcription factor: Nac" "characteristics_ch1.2"
"Cells were grown in LB media with 1mM IPTG at 37 °C with shaking for 2 hours" "growth_protocol_ch1.1"
"Escherichia coli K-12" "organism_ch1.1"
"Culture cells" "source_name_ch1.1"
"For total RNA extraction, cells were pelleted in 4 °C." "treatment_protocol_ch1.1"