"treatment: 0.5 µg/ml Carolacton"	"characteristics_ch1.1"
"genotype: TolC defective (TolC-)"	"characteristics_ch1.2"
"time point (minutes): 15"	"characteristics_ch1.3"
"Sequenced reads were trimmed for adaptor sequences using FastQC v0.11.2."	"data_processing.1"
"Raw read counts of transcripts were obtained by aligning samples 1-14 to the reference genome and counting mapped reads per feature using Rockhopper v2.0.3."	"data_processing.2"
"Genome_build: CP018801 (https://www.ncbi.nlm.nih.gov/nuccore/CP018801)"	"data_processing.3"
"Supplementary_files_format_and_content: The tab-delimited text files include raw read values for each time-point for both biol. replicates of control and Carolacton-treated samples as obtained from Rockhopper. A FASTA file on the series record contains the transcript sequences."	"data_processing.4"
"Total cellular RNAs were extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturers protocol. Residual DNA in the fractions was digested by addition of 27 Kunitz units Dnase I (Qiagen), and absence of DNA verified by Polymerase chain reaction (PCR). mRNA enrichment was conducted using the Ribo-Zero kit for Gram-negative bacteria (epicentre). RNA quality control of the RNA extracts, as well as the successful mRNA enrichment, was conducted by using an Agilent 2100 Bioanalyzer."	"extract_protocol_ch1.1"
"Libraries of RNA fractions were constructed with the ScriptSeq V2 RNA-Seq Library Prep Kit (Illumina) according to the manufacturers guidelines."	"extract_protocol_ch1.2"
"E. coli TolC cells were grown o/n in LB medium at 37°C and 200 rpm."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"planktonic cells"	"source_name_ch1.1"
"Eco_TolC_15min_Carolacton_B"	"title.1"
"An o/n culture of E. coli TolC was diluted to an OD600 of 0.1 and supplied with a final concentration of 0.5 µg/ml Carolacton in MeOH. Controls received the same volume MeOH. Three early time-points (t) were chosen to be analyzed by RNA-seq. Prior to addition of Carolacton (t0), t5, t15 and t30 min after addition of Carolacton. At each point, 5 ml of culture were mixed with an equal volume RNAprotect (Qiagen), incubated at room temperature (RT) for 5 min, the cells harvested by centrifugation, and the pellet frozen and stored at -80°C."	"treatment_protocol_ch1.1"
"RNA-Seq"	"library_strategy.1"
"treatment: 0.5 µg/ml Carolacton"	"characteristics_ch1.1"
"genotype: TolC defective (TolC-)"	"characteristics_ch1.2"
"time point (minutes): 15"	"characteristics_ch1.3"
"E. coli TolC cells were grown o/n in LB medium at 37°C and 200 rpm."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"planktonic cells"	"source_name_ch1.1"
"An o/n culture of E. coli TolC was diluted to an OD600 of 0.1 and supplied with a final concentration of 0.5 µg/ml Carolacton in MeOH. Controls received the same volume MeOH. Three early time-points (t) were chosen to be analyzed by RNA-seq. Prior to addition of Carolacton (t0), t5, t15 and t30 min after addition of Carolacton. At each point, 5 ml of culture were mixed with an equal volume RNAprotect (Qiagen), incubated at room temperature (RT) for 5 min, the cells harvested by centrifugation, and the pellet frozen and stored at -80°C."	"treatment_protocol_ch1.1"