GSE90056-GSM2396716-GPL15010-PMID:.tsv
2.63 KB
"plasmid: pBADsigma32wt" "characteristics_ch1.1"
"growth temperature: 30C" "characteristics_ch1.2"
"induction: 0.2% arabinose" "characteristics_ch1.3"
"treatment temperature: 30C" "characteristics_ch1.4"
"heat shock time: None" "characteristics_ch1.5"
"replicate: 2" "characteristics_ch1.6"
"Library strategy: Ribo-Seq" "data_processing.1"
"Basecalls performed using Illumina CASAVA." "data_processing.2"
"Reads were trimmed and adaptors removed using the fastx_toolkit." "data_processing.3"
"Reads were aligned to the MG1655 genome with Bowtie." "data_processing.4"
"The number of reads at each position in the genome was counted using in-house Python scripts." "data_processing.5"
"The number of reads mapping to each gene was counted and normalized using EdgeR." "data_processing.6"
"Genome_build: NC_000913.2" "data_processing.7"
"Supplementary_files_format_and_content: ecoli_heatshock_rawcounts.txt: Tab-delimited text file has raw read counts per kilobase million for each E. coli gene (EcoCyc gene names)." "data_processing.8"
"Supplementary_files_format_and_content: ecoli_heatshock_normalizedcounts.txt: Tab-delimited text file has EdgeR-normalized counts per kilobase million for each E. coli gene (EcoCyc gene names)." "data_processing.9"
"Cells were lysed in liquid nitrogen, then samples were taken for ribosome footprint extraction." "extract_protocol_ch1.1"
"Polysomes were digested with MNase, monosomes were purified by sucrose gradient ultracentrifugation, and the resulting mRNA fragments were extracted with phenol chloroform and gel-purified." "extract_protocol_ch1.2"
"RNA was dephosphorylated, ligated to an adaptor, reverse transcribed, circularized then amplified with Illumina adaptors and barcodes." "extract_protocol_ch1.3"
"E. coli were grown in defined rich media at 30C." "growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655" "organism_ch1.1"
"E. coli cultures" "source_name_ch1.1"
"ecoli_k12_pBADsigma32wt_30C_f_2" "title.1"
"Cells were induced with arabinose or heat shocked, then harvested and flash frozen." "treatment_protocol_ch1.1"
"OTHER" "library_strategy.1"
"plasmid: pBADsigma32wt" "characteristics_ch1.1"
"growth temperature: 30C" "characteristics_ch1.2"
"induction: 0.2% arabinose" "characteristics_ch1.3"
"treatment temperature: 30C" "characteristics_ch1.4"
"heat shock time: None" "characteristics_ch1.5"
"replicate: 2" "characteristics_ch1.6"
"E. coli were grown in defined rich media at 30C." "growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655" "organism_ch1.1"
"E. coli cultures" "source_name_ch1.1"
"Cells were induced with arabinose or heat shocked, then harvested and flash frozen." "treatment_protocol_ch1.1"