"plasmid: pBAD"	"characteristics_ch1.1"
"growth temperature: 30C"	"characteristics_ch1.2"
"induction: 0.2% arabinose"	"characteristics_ch1.3"
"treatment temperature: 30C"	"characteristics_ch1.4"
"heat shock time: None"	"characteristics_ch1.5"
"replicate: 2"	"characteristics_ch1.6"
"Basecalls performed using Illumina CASAVA."	"data_processing.1"
"Reads were trimmed and adaptors removed using the fastx_toolkit."	"data_processing.2"
"Reads were aligned to the MG1655 genome with Bowtie."	"data_processing.3"
"The number of reads at each position in the genome was counted using in-house Python scripts."	"data_processing.4"
"The number of reads mapping to each gene was counted and normalized using EdgeR."	"data_processing.5"
"Genome_build: NC_000913.2"	"data_processing.6"
"Supplementary_files_format_and_content: ecoli_heatshock_rawcounts.txt: Tab-delimited text file has raw read counts per kilobase million for each E. coli gene (EcoCyc gene names)."	"data_processing.7"
"Supplementary_files_format_and_content: ecoli_heatshock_normalizedcounts.txt: Tab-delimited text file has EdgeR-normalized counts per kilobase million for each E. coli gene (EcoCyc gene names)."	"data_processing.8"
"Cells were lysed in liquid nitrogen, then samples were taken for total RNA."	"extract_protocol_ch1.1"
"Total RNA was extracted with phenol chloroform and alkaline fragmented, then fragments (20-40 nt) were gel-purified."	"extract_protocol_ch1.2"
"RNA was dephosphorylated, ligated to an adaptor, reverse transcribed, circularized then amplified with Illumina adaptors and barcodes."	"extract_protocol_ch1.3"
"E. coli were grown in defined rich media at 30C."	"growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"E. coli cultures"	"source_name_ch1.1"
"ecoli_k12_pBAD_30C_m_2"	"title.1"
"Cells were induced with arabinose or heat shocked, then harvested and flash frozen."	"treatment_protocol_ch1.1"
"RNA-Seq"	"library_strategy.1"
"plasmid: pBAD"	"characteristics_ch1.1"
"growth temperature: 30C"	"characteristics_ch1.2"
"induction: 0.2% arabinose"	"characteristics_ch1.3"
"treatment temperature: 30C"	"characteristics_ch1.4"
"heat shock time: None"	"characteristics_ch1.5"
"replicate: 2"	"characteristics_ch1.6"
"E. coli were grown in defined rich media at 30C."	"growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655"	"organism_ch1.1"
"E. coli cultures"	"source_name_ch1.1"
"Cells were induced with arabinose or heat shocked, then harvested and flash frozen."	"treatment_protocol_ch1.1"