GSE88980-GSM2356690-GPL17439-PMID:28526842.tsv
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"strain: K-12 MG1655" "characteristics_ch1.1"
"genotype: ompR deletion mutant" "characteristics_ch1.2"
"Sequenced reads were mapped onto NC_000913 reference genome sequence using bowtie v1.0.0 with parameters -X 1000 -n 2 -3 3 -S" "data_processing.1"
"Fragments Per Kilobase of exon per Megabase of library size (FPKM) were calculated using cufflinks v.1.3.0" "data_processing.2"
"Genome_build: NC_000913" "data_processing.3"
"Supplementary_files_format_and_content: comma-delimited text files include FPKM values for each Sample." "data_processing.4"
"Total RNA was extracted using the RNeasy Plus Mini kit (Qiagen Inc., Valencia, CA, USA) and genomic DNA was removed by gDNA Eliminator spin column in the RNeasy Plus Mini Kit. RNA quality and concentration was determined by analysis with a NanoDrop 1000 (Thermo Scientific Inc., Wilmington, DE, USA)." "extract_protocol_ch1.1"
"RNA libraries were prepared for sequencing using standard Illumina protocols" "extract_protocol_ch1.2"
"E. coli K-12 MG1655 WT and ΔompR were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 0.3 M of NaCl at mid-log pahse for 30 min with agitation." "growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655" "organism_ch1.1"
"Total RNA isolated from E. coli" "source_name_ch1.1"
"ΔompR NaCl 2" "title.1"
"The cell culture was treated with the RNAprotect reagent (Qiagen)." "treatment_protocol_ch1.1"
"RNA-Seq" "library_strategy.1"
"strain: K-12 MG1655" "characteristics_ch1.1"
"genotype: ompR deletion mutant" "characteristics_ch1.2"
"E. coli K-12 MG1655 WT and ΔompR were grown to mid-log phase aerobically at 37°C in M9 minimal media supplemented with 0.2% glucose. Then cells were treated with 0.3 M of NaCl at mid-log pahse for 30 min with agitation." "growth_protocol_ch1.1"
"Escherichia coli str. K-12 substr. MG1655" "organism_ch1.1"
"Total RNA isolated from E. coli" "source_name_ch1.1"
"The cell culture was treated with the RNAprotect reagent (Qiagen)." "treatment_protocol_ch1.1"