GSE87856-GSM2341962-GPL14548-PMID:28115545.tsv
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"strain: DMS2545 (K-12 derivative)" "characteristics_ch1.1"
"rpos level: 0%" "characteristics_ch1.2"
"Base calls performed with RTA v1.18.5" "data_processing.1"
"the first ten base pairs of each read were trimmed using FASTXToolkit 0.0.13." "data_processing.2"
"Reads were mapped using BWA 0.7.5a" "data_processing.3"
"The number read pairs mapped to each gene was counted with HTSeq 0.6.1" "data_processing.4"
"Supplementary_files_format_and_content: A tab deliminited file with counts for each gene." "data_processing.5"
"RNA was purified from 200 µL of overnight culture by pelleting and resuspeding in 500 µL of Trizol at 65°C, followed by purification on a column (Direct-Zol, Zymo Research). Samples received two 30-minute DNase treatments using TURBO DNA-free (Ambion) following the manufacturers instructions. RNA samples were then purified on a column (RNA Clean & Concentrator, Zymo Research). Three samples were prepped from each culture and pooled to generate sufficient RNA." "extract_protocol_ch1.1"
"RNA libraries were prepared for sequencing using standard Illumina protocols" "extract_protocol_ch1.2"
"Cells were inoculated from frozen cultures into 5 mL of LB, and grown. 5 µL of this overnight culture was diluted into 5 mL of LB with the appropriate concentration of arabinose, and grown for 20h." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"E. coli culture" "source_name_ch1.1"
"0% rep1" "title.1"
"RNA-Seq" "library_strategy.1"
"strain: DMS2545 (K-12 derivative)" "characteristics_ch1.1"
"rpos level: 0%" "characteristics_ch1.2"
"Cells were inoculated from frozen cultures into 5 mL of LB, and grown. 5 µL of this overnight culture was diluted into 5 mL of LB with the appropriate concentration of arabinose, and grown for 20h." "growth_protocol_ch1.1"
"Escherichia coli" "organism_ch1.1"
"E. coli culture" "source_name_ch1.1"