GSE85807-GSM2284716-GPL13360-PMID:28642478.tsv 1.82 KB
"culture temperature: 42°C"	"characteristics_ch1.1"
"vector: pET-28a"	"characteristics_ch1.2"
"Raw data were normalized (quantile method), merged and filtered by Feature Extraction Software (Agilent Technologies Inc.). For the sibiling probes (multiple probes to one gene), we used the median indensity for representation."	"data_processing.1"
"Collected cells were lysed using Trizol (Invitrogen), and total RNA was isolated using phenol-chloroform and precipitated with ice cold isopropanol. Then the precipitations were further washed with 70% ethanol and dissolved in RNase-free water. Genomic DNA was removed using RNase-free DNase I. RNA samples were further purified using RNeasy Mini Kits (Qiagen). RNA concentration and quality were determined using A Nanodrop ND 1000 spectrophotometer (Thermo Fisher Scientific, Pittsburgh, PA, USA) and agarose gel electrophoresis. The isolated RNA was stored at −80°C before use."	"extract_protocol_ch1.1"
"E. coli Rosetta cells transformed with pET-28a or pET-28a-BnTR1 (OD600nm = 0.6) were induced with 0.1 mM IPTG at 37°C for 1 hour."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Emplty Vector, 42°C, replicate 2"	"source_name_ch1.1"
"EmptyVector_42_rep2"	"title.1"
"E. coli cells with or without BnTR1 were transferred and incubated at 37°C and 42°C for 1 hour, respectively"	"treatment_protocol_ch1.1"
"culture temperature: 42°C"	"characteristics_ch1.1"
"vector: pET-28a"	"characteristics_ch1.2"
"E. coli Rosetta cells transformed with pET-28a or pET-28a-BnTR1 (OD600nm = 0.6) were induced with 0.1 mM IPTG at 37°C for 1 hour."	"growth_protocol_ch1.1"
"Escherichia coli"	"organism_ch1.1"
"Emplty Vector, 42°C, replicate 2"	"source_name_ch1.1"
"E. coli cells with or without BnTR1 were transferred and incubated at 37°C and 42°C for 1 hour, respectively"	"treatment_protocol_ch1.1"